nat10 (SPAC20G8.09c)


Gene Standard Namenat10 Characterisation Statuspublished
Systematic IDSPAC20G8.09c Feature Typeprotein coding
Synonyms Name Description
Productribosome biogenesis ATPase Product Size1033aa, 116.46 kDa
Genomic Location Chromosome I, 1418825-1415452 (3374nt); CDS:1418750-1415542 (3209nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037558
GO:0016887ATPase activityISMPFAM:PF05127GO_REF:0000024232
GO:0008080N-acetyltransferase activityIEAIPR000182GO_REF:000000230
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006995cellular response to nitrogen starvationIMPPMID:1936672885
GO:0070317negative regulation of G0 to G1 transitionIMPPMID:1936672825
GO:0042274ribosomal small subunit biogenesisISOSGD:S000005076GO_REF:000002454
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:003068690S preribosomeISOSGD:S000005076GO_REF:000002416
GO:0005730nucleolusIDAPMID:16823372357
ISOSGD:S000005076GO_REF:0000024
GO:0005634nucleusIDAPMID:168233722737
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000082decreased cell population growth at high temperatureCell growth assaynat10-447PECO:0000004, PECO:0000099PMID:19366728144
FYPO:0000709decreased cell population growth during nitrogen starvationCell growth assaynat10-447PECO:0000004, PECO:0000099PMID:1936672835
FYPO:0002060viable vegetative cell populationMicroscopynat10ΔNullPECO:0000005, PECO:0000137PMID:236978063751
Microscopynat10ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000997pear-shaped cell during nitrogen starvationMicroscopynat10-447PECO:0000005, PECO:0000090PMID:193667286
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopynat10ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
114188251418694
214186541418509
314184401415452
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13725 Pfam IPR027992 Possible tRNA binding domain 769 893 1
PF08351 Pfam IPR013562 Domain of unknown function DUF1726 108 202 1
PF05127 Pfam IPR007807 Helicase domain 280 483 1
PF13718 Pfam IPR000182 GNAT domain 525 754 1
PTHR10925 HMMPANTHER 2 1033 1
PTHR10925:SF5 HMMPANTHER 2 1033 1
Coil ncoils Rabaptin coiled-coil domain 945 966 968

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.74 Da
Charge 13.50
Isoelectric point 7.90
Molecular weight 116.46 kDa
Number of residues 1033
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS943PMID:217125471670
present during mitotic M phase
present during mitotic M phaseS923PMID:21712547
level fluctuates during mitotic cell cycleS1013PMID:24763107
present during mitotic M phaseS1017PMID:21712547
S923PMID:24763107
present during mitotic M phaseS1013PMID:21712547
MOD:00047O-phospho-L-threonineT938PMID:21712547690
present during mitotic M phase
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA leveldecreased in presence of cadmium sulfate expression microarray evidencePMID:12529438
decreased during GO:0034605expression microarray evidencePMID:12529438
decreased during GO:0034599expression microarray evidencePMID:12529438
decreased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
decreased during GO:0071470expression microarray evidencePMID:12529438

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
23412during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
22773during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
24366during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
21250during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
22812during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
7562.8during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
5475.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.82during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
prw1Clr6 histone deacetylase complex subunit Prw1 Positive GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
far8SIP/FAR complex striatin subunit, Far8/Csc3 Affinity Capture-MSPMID:22119525
External References
Database Identifier Description
NBRP SPAC20G8.09c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC20G8.09c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC20G8.09c BioGRID Interaction Datasets
Expression Viewer SPAC20G8.09c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC20G8.09c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC20G8.09c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC20G8.09c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC20G8.09c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC20G8.09c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC20G8.09c Cell Cycle Data
GEO SPAC20G8.09c GEO profiles
PInt SPAC20G8.09c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC20G8.09c Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN48/48A10Orfeome Localization Data
UniProtKB/SwissProtP87115UPF0202 protein C20G8.09c
ModBaseP87115Database of comparative protein structure models
STRINGP87115Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593326ribosome biogenesis ATPase (predicted)
RefSeq mRNANM_001018757972h- ribosome biogenesis ATPase (predicted) (SPAC20G8.09c), mRNA
European Nucleotide ArchiveCAB08603.1ENA Protein Mapping
UniParcUPI0000037D5FUniProt Archive

Literature for nat10

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014