nat10 (SPAC20G8.09c)

Gene Standard Namenat10 Characterisation Statuspublished
Systematic IDSPAC20G8.09c Feature Typeprotein coding
Synonyms Name Description
Productribosome biogenesis ATPase Product Size1033aa, 116.46 kDa
Genomic Location Chromosome I, 1418825-1415452 (3374nt); CDS:1418750-1415542 (3209nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
ATPase activity232
Annotation ExtensionEvidenceWith/FromReference
N-acetyltransferase activity30
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

ribosome biogenesis

Term NameCount
cellular response to nitrogen starvation86
Annotation ExtensionEvidenceWith/FromReference
negative regulation of G0 to G1 transition25
Annotation ExtensionEvidenceWith/FromReference
ribosomal small subunit biogenesis54
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
90S preribosome17
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000082decreased cell population growth at high temperatureCell growth assaynat10-447PECO:0000004, PECO:0000099PMID:19366728148
FYPO:0000709decreased cell population growth during nitrogen starvationCell growth assaynat10-447PECO:0000004, PECO:0000099PMID:1936672835
FYPO:0002060viable vegetative cell populationMicroscopynat10ΔNullPECO:0000005, PECO:0000137PMID:236978063759

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000997pear-shaped cell during nitrogen starvationMicroscopynat10-447PECO:0000005, PECO:0000090PMID:193667286
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopynat10ΔNullPECO:0000005, PECO:0000137PMID:23697806
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13725 Pfam IPR027992 Possible tRNA binding domain 769 893 1
PF08351 Pfam IPR013562 Domain of unknown function DUF1726 108 202 1
PF05127 Pfam IPR007807 Helicase domain 280 483 1
PF13718 Pfam IPR000182 GNAT domain 525 754 1
PTHR10925 HMMPANTHER 2 1033 1
PTHR10925:SF5 HMMPANTHER 2 1033 1
Coil ncoils Rabaptin coiled-coil domain 945 966 968

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.74 Da
Charge 13.50
Isoelectric point 7.90
Molecular weight 116.46 kDa
Number of residues 1033

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS1017PMID:217125471670
present during mitotic M phase
level fluctuates during mitotic cell cycleexperimental evidenceS1013PMID:24763107
experimental evidenceS923PMID:24763107
present during mitotic M phaseexperimental evidenceS1013PMID:21712547
present during mitotic M phaseexperimental evidenceS923PMID:21712547
present during mitotic M phaseexperimental evidenceS943PMID:21712547
MOD:00047O-phospho-L-threonineexperimental evidenceT938PMID:21712547692
present during mitotic M phase
Gene Expression

Qualitative Gene Expression

RNA leveldecreased during GO:0034599expression microarray evidencePMID:12529438
decreased during GO:0071470expression microarray evidencePMID:12529438
decreased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
decreased in presence of cadmium sulfate expression microarray evidencePMID:12529438
decreased during GO:0034605expression microarray evidencePMID:12529438

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
23412during GO:0000080PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
22773during GO:0000084PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
24366during GO:0000085PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
21250during GO:0000087PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
22812during GO:0072690PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
7562.8during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
5475.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
0.82during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
mtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
pir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
far8SIP/FAR complex striatin subunit, Far8/Csc3 Affinity Capture-MSPMID:22119525
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
red1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
red5human ZC3H3 homolog Affinity Capture-MSPMID:24713849
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
prw1Clr6 histone deacetylase complex subunit Prw1 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC20G8.09c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC20G8.09c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC20G8.09c BioGRID Interaction Datasets
Expression Viewer SPAC20G8.09c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC20G8.09c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC20G8.09c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC20G8.09c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC20G8.09c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC20G8.09c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC20G8.09c Cell Cycle Data
GEO SPAC20G8.09c GEO profiles
PInt SPAC20G8.09c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC20G8.09c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC20G8.09c Fission yeast phenotypic data & analysis
SPD / RIKEN48/48A10Orfeome Localization Data
UniProtKB/SwissProtP87115UPF0202 protein C20G8.09c
ModBaseP87115Database of comparative protein structure models
STRINGP87115Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593326ribosome biogenesis ATPase (predicted)
RefSeq mRNANM_001018757972h- ribosome biogenesis ATPase (predicted) (SPAC20G8.09c), mRNA
European Nucleotide ArchiveCAB08603.1ENA Protein Mapping
UniParcUPI0000037D5FUniProt Archive

Literature for nat10

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014