nat10 (SPAC20G8.09c)


Gene Standard Namenat10 Characterisation Statuspublished
Systematic IDSPAC20G8.09c Feature Typeprotein coding
Synonyms Name Description
Productribosome biogenesis ATPase Product Size1033aa, 116.46 kDa
Genomic Location Chromosome I, 1418825-1415452 (3374nt); CDS:1418750-1415542 (3209nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding559
Annotation ExtensionEvidenceWith/FromReference
rRNA cytidine N-acetyltransferase activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

ribosome biogenesis


Term NameCount
negative regulation of G0 to G1 transition23
Annotation ExtensionEvidenceWith/FromReference
ribosomal small subunit biogenesis55
Annotation ExtensionEvidenceWith/FromReference
rRNA acetylation involved in maturation of SSU-rRNA1
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
90S preribosome20
Annotation ExtensionEvidenceWith/FromReference
nucleolus366
Annotation ExtensionEvidenceWith/FromReference
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viability depends on conditions

Population Phenotype

Term NameGenotypesCount
decreased cell population growth at high temperaturenat10-447 (G285D)173
inviable vegetative cell populationnat10Δ1453
loss of viability upon nitrogen starvationnat10-447 (G285D)53
slow vegetative cell population growthnat10-447 (G285D)357
viable vegetative cell populationnat10Δ3837

Cell Phenotype

Term NameGenotypesCount
abnormal ribosome biogenesisnat10-447 (G285D)1
abolished 18S rRNA acetylationnat10-447 (G285D)1
viable vegetative cell with normal cell morphologynat10Δ3100
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1418825..1418694, 1418654..1418509, 1418440..1415452
Intron1418693..1418655, 1418508..1418441
5' UTR1418825..1418751PMID:21511999
3' UTR1415541..1415452AU009291
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08351 Pfam IPR013562 tRNA(Met) cytidine acetyltransferase TmcA, N-terminal 108 202 1
PF05127 Pfam IPR007807 Helicase domain 280 483 1
PF13725 Pfam IPR027992 Possible tRNA binding domain 769 894 1
PF13718 Pfam IPR000182 GNAT domain 525 754 1
PTHR10925 HMMPANTHER IPR032672 TmcA/NAT10/Kre33 2 1033 1
PTHR10925:SF5 HMMPANTHER 2 1033 1
Coil ncoils Predicted coiled-coil protein (DUF2205) 945 965 1057

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.74 Da
Charge 13.50
Codon Adaptation Index 0.44
Isoelectric point 7.90
Molecular weight 116.46 kDa
Number of residues 1033
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phaseS923
present during mitotic M phaseS943
present during mitotic M phaseS1013
present during mitotic M phaseS1017
level fluctuates during mitotic cell cycleS1013
S923
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S923 PMID:21712547
present during mitotic M phase experimental evidence S943 PMID:21712547
present during mitotic M phase experimental evidence S1013 PMID:21712547
present during mitotic M phase experimental evidence S1017 PMID:21712547
level fluctuates during mitotic cell cycle experimental evidence S1013 PMID:24763107
experimental evidence S923 PMID:24763107
O-phospho-L-threonine 700
present during mitotic M phaseT938
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T938 PMID:21712547
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA leveldecreased during GO:0071470expression microarray evidencePMID:12529438
decreased in presence of cadmium sulfate expression microarray evidencePMID:12529438
decreased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
decreased during GO:0034599expression microarray evidencePMID:12529438
decreased during GO:0034605expression microarray evidencePMID:12529438

Quantitative Gene Expression

View graphical display of gene expression data for nat10 (SPAC20G8.09c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
23412during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
22773during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
24366during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
21250during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
7562.8during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
22812during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5475.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.82during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
acetylcytidine at positions 1297 & 1815 PMID:254024801
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4605
conserved in eukaryotes4515
conserved in metazoa3424
conserved in vertebrates3399
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC20G8.09c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bymtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
affinity captured byfar8SIP/FAR complex striatin subunit, Far8/Csc3 Affinity Capture-MSPMID:22119525
affinity captured byred5human ZC3H3 homolog Affinity Capture-MSPMID:24713849
affinity captured bypir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
affinity captured byred1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC20G8.09c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Positive GeneticPMID:22681890
negative genetic interaction withptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC20G8.09c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC20G8.09c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC20G8.09c BioGRID Interaction Datasets
Expression Viewer SPAC20G8.09c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC20G8.09c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC20G8.09c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC20G8.09c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC20G8.09c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC20G8.09c Transcriptome Viewer (Bähler Lab)
GEO SPAC20G8.09c GEO profiles
PInt SPAC20G8.09c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC20G8.09c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC20G8.09c Fission yeast phenotypic data & analysis
Cyclebase SPAC20G8.09c.1 Cell Cycle Data
SPD / RIKEN48/48A10Orfeome Localization Data
UniProtKB/SwissProtP87115RNA cytidine acetyltransferase
ModBaseP87115Database of comparative protein structure models
STRINGP87115Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593326ribosome biogenesis ATPase (predicted)
RefSeq mRNANM_001018757972h- ribosome biogenesis ATPase (predicted) (SPAC20G8.09c), mRNA

Literature for nat10

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016