nat10 (SPAC20G8.09c)


Gene Standard Namenat10 Characterisation Statuspublished
Systematic IDSPAC20G8.09c Feature Typeprotein coding
Synonyms Name Description
Productribosome biogenesis ATPase Product Size1033aa, 116.46 kDa
Genomic Location Chromosome I, 1418825-1415452 (3374nt); CDS:1418750-1415542 (3209nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
N-acetyltransferase activity30
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

ribosome biogenesis


Term NameCount
cellular response to nitrogen starvation82
Annotation ExtensionEvidenceWith/FromReference
negative regulation of G0 to G1 transition25
Annotation ExtensionEvidenceWith/FromReference
ribosomal small subunit biogenesis55
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
90S preribosome20
Annotation ExtensionEvidenceWith/FromReference
nucleolus359
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growth at high temperaturenat10-447151
decreased cell population growth during nitrogen starvationnat10-44735
viable vegetative cell populationnat10ΔNull3781

Cell Phenotype

Term NameAlleleExpressionCount
pear-shaped cell during nitrogen starvationnat10-4476
viable vegetative cell with normal cell morphology3092
penetrance FYPO_EXT:0000001nat10ΔNull
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
114188251418694
214186541418509
314184401415452

UTRs

Region Coordinates Reference
five_prime_UTR1418825..1418751PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13718 Pfam IPR000182 GNAT domain 525 754 1
PF08351 Pfam IPR013562 Domain of unknown function DUF1726 108 202 1
PF05127 Pfam IPR007807 Helicase domain 280 483 1
PF13725 Pfam IPR027992 Possible tRNA binding domain 769 893 1
PTHR10925 HMMPANTHER 1 1033 1
PTHR10925:SF5 HMMPANTHER 1 1033 1
Coil ncoils Rabaptin coiled-coil domain 945 966 971

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.74 Da
Charge 13.50
Isoelectric point 7.90
Molecular weight 116.46 kDa
Number of residues 1033
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS1013
present during mitotic M phaseS1017
S923
present during mitotic M phaseS943
present during mitotic M phaseS923
level fluctuates during mitotic cell cycleS1013
Annotation ExtensionEvidenceResidueReference
experimental evidence S923 PMID:24763107
present during mitotic M phase experimental evidence S923 PMID:21712547
present during mitotic M phase experimental evidence S943 PMID:21712547
present during mitotic M phase experimental evidence S1013 PMID:21712547
level fluctuates during mitotic cell cycle experimental evidence S1013 PMID:24763107
present during mitotic M phase experimental evidence S1017 PMID:21712547
O-phospho-L-threonine 693
present during mitotic M phaseT938
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T938 PMID:21712547
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA leveldecreased during GO:0034599expression microarray evidencePMID:12529438
decreased during GO:0034605expression microarray evidencePMID:12529438
decreased in presence of cadmium sulfate expression microarray evidencePMID:12529438
decreased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
decreased during GO:0071470expression microarray evidencePMID:12529438

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
23412during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
22773during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
24366during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
21250during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
7562.8during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
22812during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5475.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.82during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
mtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
pir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
far8SIP/FAR complex striatin subunit, Far8/Csc3 Affinity Capture-MSPMID:22119525
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
red1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
red5human ZC3H3 homolog Affinity Capture-MSPMID:24713849
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
prw1Clr6 histone deacetylase complex subunit Prw1 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC20G8.09c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC20G8.09c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC20G8.09c BioGRID Interaction Datasets
Expression Viewer SPAC20G8.09c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC20G8.09c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC20G8.09c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC20G8.09c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC20G8.09c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC20G8.09c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC20G8.09c Cell Cycle Data
GEO SPAC20G8.09c GEO profiles
PInt SPAC20G8.09c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC20G8.09c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC20G8.09c Fission yeast phenotypic data & analysis
SPD / RIKEN48/48A10Orfeome Localization Data
UniProtKB/SwissProtP87115UPF0202 protein C20G8.09c
ModBaseP87115Database of comparative protein structure models
STRINGP87115Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593326ribosome biogenesis ATPase (predicted)
RefSeq mRNANM_001018757972h- ribosome biogenesis ATPase (predicted) (SPAC20G8.09c), mRNA
European Nucleotide ArchiveCAB08603.1ENA Protein Mapping
UniParcUPI0000037D5FUniProt Archive

Literature for nat10

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015