pcr1 (SPAC21E11.03c)


Gene Standard Namepcr1 Characterisation Statuspublished
Systematic IDSPAC21E11.03c Feature Typeprotein coding
Synonymsmts2 Name Description
Producttranscription factor Pcr1 Product Size171aa, 19.35 kDa
Genomic Location Chromosome I, 4253622-4252142 (1481nt); CDS:4252918-4252403 (516nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0003677DNA bindingIDAPMID:7958849385
GO:0003723RNA bindingIDAPMID:7518718648
GO:0000978RNA polymerase II core promoter proximal region sequence-specific DNA bindingIDAPMID:1544813794
binds CREIDAPMID:9135083
IDAPMID:8557039
at M26_binding_siteIDAPMID:15448137
GO:0000982RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activityIMPPMID:855209991
GO:0001077RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription28
during cellular response to cadmium ion, regulates ntp1IMPPMID:15164362
during cellular response to glucose starvation, regulates fbp1, in presence of maltoseIMPPMID:24224056
during cellular response to glucose starvation, regulates gpx1, in presence of maltoseIMPPMID:24224056
during cellular response to glucose starvation, regulates agl1, in presence of maltoseIMPPMID:24224056
GO:0046982protein heterodimerization activityIPIatf1PMID:913508327
GO:0010844recombination hotspot bindingIDAPMID:79588492
GO:0043565sequence-specific DNA bindingIDAPMID:7958849141
binds CREIDAPMID:8552099
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006995cellular response to nitrogen starvationIMPPMID:1544813785
GO:1990376negative regulation of G1/S transition of mitotic cell cycle by positive regulation of transcription from RNA polymerase II promoter in response to nitrogen starvationIMPPMID:91350832
GO:0045944positive regulation of transcription from RNA polymerase II promoterIMPPMID:2422405681
GO:0045893positive regulation of transcription, DNA-templated91
during meiotic cell cycleIDAPMID:8552099
GO:0007131reciprocal meiotic recombinationIMPPMID:939110138
GO:0043949regulation of cAMP-mediated signalingIMPPMID:154481378
GO:1900391regulation of cAMP-mediated signaling by regulation of transcription from RNA polymerase II promoter2
regulates cgs2IMPPMID:15448137
GO:0010847regulation of chromatin assemblyIMPPMID:1498873213
GO:0010848regulation of chromatin disassemblyIMPPMID:154481372
GO:0035065regulation of histone acetylationIMPPMID:149887328
GO:0031060regulation of histone methylationIMPPMID:1521815027
GO:0006357regulation of transcription from RNA polymerase II promoterIMPPMID:8552099262
GO:0006366transcription from RNA polymerase II promoterIMPPMID:24224056139
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:1990243atf1-pcr1 complexIGIatf1PMID:242240562
GO:0072686mitotic spindleIDAPMID:16823372254
GO:0000790nuclear chromatinNASGO_REF:0000001278
GO:0005634nucleusIDAPMID:168233722737
GO:0005667transcription factor complex61
localization requires atf1IDAPMID:9135083
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002924decreased cell population growth on maltose carbon sourceCell growth assaypcr1ΔNullPECO:0000236PMID:242240563
FYPO:0000073resistance to caffeine20
expressivity FYPO_EXT:0000001Cell growth assaypcr1ΔNullPECO:0000137PMID:19672306
FYPO:0001103resistance to hydrogen peroxide63
expressivity FYPO_EXT:0000001Cell growth assaypcr1ΔNullPECO:0000137PMID:19672306
FYPO:0000096sensitive to cadmium246
expressivity FYPO_EXT:0000003Cell growth assaypcr1ΔNullPECO:0000102, PECO:0000005, PECO:0000137PMID:18684775
FYPO:0000080sensitive to coldCell growth assaypcr1ΔNullPECO:0000102, PECO:0000137PMID:855209942
FYPO:0002060viable vegetative cell populationCell growth assaypcr1ΔNullPECO:0000005, PECO:0000137, PECO:0000102PMID:85520993751
Microscopypcr1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Cell growth assaypcr1ΔNullPECO:0000006, PECO:0000126, PECO:0000102PMID:8552099
Microscopypcr1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000710abnormal cell cycle arrest in response to nitrogen starvation19
penetrance FYPO_EXT:0000001Flow cytometry datapcr1ΔNullPMID:9135083
FYPO:0000054abnormal microtubule cytoskeleton organizationMicroscopynot recorded (overexpression)PMID:1579792551
FYPO:0002003abolished RNA polymerase II core promoter proximal region sequence-specific DNA binding5
affecting SO:0001843 and atf1Gel electrophoresis evidencepcr1ΔNullPMID:9135083
FYPO:0000658decreased DNA binding9
affecting SO:0001843Gel electrophoresis evidencepcr1T (N-term half of basic region deleted)Not specifiedPMID:8552099
FYPO:0001116decreased RNA level during cellular response to hydrogen peroxide18
affecting tps1Transcript expression level evidencepcr1ΔNullPMID:12855726
affecting ntp1Transcript expression level evidencepcr1ΔNullPMID:12855726
FYPO:0002304decreased RNA level during cellular response to salt stress8
affecting ntp1Transcript expression level evidencepcr1ΔNullPECO:0000166PMID:12855726
affecting tps1Transcript expression level evidencepcr1ΔNullPECO:0000166PMID:12855726
FYPO:0001152decreased RNA level during nitrogen starvation26
affecting ste11Transcript expression level evidencepcr1ΔNullPMID:8552099
FYPO:0000708decreased mating efficiencyCell growth assaypcr1ΔNullPMID:8552099238
Cell growth assaypcr1T (N-term half of basic region deleted)Not specifiedPMID:8552099
FYPO:0002925decreased protein localization to M26 site2
affecting med7Chromatin immunoprecipitation experimentpcr1ΔNullPECO:0000236PMID:24224056
FYPO:0000584decreased sporulationCell growth assaypcr1-/pcr1- (homozygous diploid)NullPMID:855209952
FYPO:0000781decreased transcription during vegetative growth20
affecting fbp1Quantitative PCRpcr1ΔNullPECO:0000236PMID:24224056
affecting agl1Quantitative PCRpcr1ΔNullPECO:0000236PMID:24224056
affecting agl1Quantitative PCRpcr1ΔNullPECO:0000236PMID:24224056
FYPO:0001666normal cAMP levelSubstance quantification evidencepcr1ΔNullPMID:85520993
FYPO:0001492viable elongated vegetative cellMicroscopypcr1ΔNullPMID:8552099197
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopypcr1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in atf1 transcription factor, Atf-CREB family Atf1 PMID:9135083
FYPO affected by mutation in ctu2 cytosolic thiouridylase subunit Ctu2 PMID:23874237
FYPO affected by mutation in elp3 elongator complex subunit Elp3 (predicted) PMID:23874237
FYPO affected by mutation in pka1 cAMP-dependent protein kinase catalytic subunit Pka1 PMID:8552099
GO localised by atf1 transcription factor, Atf-CREB family Atf1 PMID:9135083
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
142536224252142
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00170 Pfam IPR004827 Basic-leucine zipper domain 12 69 5
SM00338 SMART IPR004827 Basic-leucine zipper domain 8 72 5
PS50217 Prosite Profiles IPR004827 Basic-leucine zipper domain 10 73 6
PS00036 Prosite Patterns IPR004827 Basic-leucine zipper domain 15 30 5
PTHR19304:SF5 HMMPANTHER 5 171 2
PTHR19304 HMMPANTHER 5 171 4
1.20.5.170 Gene3D 5 74 7
SSF57959 SuperFamily 12 72 6
Coil ncoils Rabaptin coiled-coil domain 35 70 968

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000545bZIP (basic leucine zipper) transcription factor familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000545
PBO:0002433Atf-CREB subfamilyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0002433

Protein Properties

Ave. residue weight 113.15 Da
Charge 11.50
Isoelectric point 10.53
Molecular weight 19.35 kDa
Number of residues 171
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelincreased during GO:0070301Western blot evidencePMID:23874237
present during GO:0072690Western blot evidencePMID:23874237
RNA levelincreased during GO:0034605expression microarray evidencePMID:12529438
increased during GO:0071470expression microarray evidencePMID:12529438
increased during GO:0034599expression microarray evidencePMID:12529438
increased in presence of cadmium sulfate expression microarray evidencePMID:12529438
increased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
increased during GO:0070301Northern assay evidencePMID:23874237

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2609.25during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
5039during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1798.96during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.5during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.69during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Subunit Composition
DescriptionQualifierReferenceCount
homomeric(2) PMID:795884944
heteromeric(2) PMID:795884942
Misc
DescriptionQualifierReferenceCount
deletion mutant expression profiling PMID:1825272178
Pathway
DescriptionQualifierReferenceCount
links stress-activated MAPK (Sty1) pathway to cAMP-dependent protein kinase (Pka1) pathway PMID:154481373
Species Distribution
DescriptionQualifierReferenceCount
orthologs cannot be distinguished131
conserved in fungi4599
conserved in eukaryotes4514
conserved in eukaryotes only2498
Orthologs

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
sre2membrane-tethered transcription factor Sre2 Positive GeneticPMID:21504829
atf1transcription factor, Atf-CREB family Atf1 Phenotypic EnhancementPMID:18375616
Dosage RescuePMID:10102365
pka1cAMP-dependent protein kinase catalytic subunit Pka1 Phenotypic SuppressionPMID:8552099
tup11transcriptional corepressor Tup11 Phenotypic SuppressionPMID:11238405
sla1La protein homolog Synthetic RescuePMID:22160596
ago1argonaute Phenotypic EnhancementPMID:15218150
deb1transcription factor Deb1/Rdp1 Phenotypic EnhancementPMID:15218150
tas3RITS complex subunit 3 Synthetic Growth DefectPMID:15475954
ers1RNA-silencing factor Ers1 Phenotypic EnhancementPMID:22733737
dcr1dicer Synthetic Growth DefectPMID:15475954
Phenotypic EnhancementPMID:15218150
chp1chromodomain protein Chp1 Synthetic Growth DefectPMID:15475954
tup12transcriptional corepressor Tup12 Phenotypic SuppressionPMID:11238405
ste11transcription factor Ste11 Phenotypic SuppressionPMID:8552099
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
swi6HP1 family chromodomain protein Swi6 Affinity Capture-WesternPMID:15292231
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
atf1transcription factor, Atf-CREB family Atf1 Affinity Capture-WesternPMID:18375616
Affinity Capture-RNAPMID:22144913
Affinity Capture-WesternPMID:9135083
External References
Database Identifier Description
NBRP SPAC21E11.03c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC21E11.03c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC21E11.03c BioGRID Interaction Datasets
Expression Viewer SPAC21E11.03c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC21E11.03c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC21E11.03c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC21E11.03c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC21E11.03c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC21E11.03c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC21E11.03c Cell Cycle Data
GEO SPAC21E11.03c GEO profiles
PInt SPAC21E11.03c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC21E11.03c Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN05/05A11Orfeome Localization Data
UniProtKB/SwissProtQ09926Transcription factor pcr1
ModBaseQ09926Database of comparative protein structure models
STRINGQ09926Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594500transcription factor Pcr1
RefSeq mRNANM_001019929972h- transcription factor Pcr1 (pcr1), mRNA
European Nucleotide ArchiveAAB46991.1ENA Protein Mapping
European Nucleotide ArchiveBAA09818.1ENA Protein Mapping
European Nucleotide ArchiveCAA91968.1ENA Protein Mapping
UniParcUPI000013144DUniProt Archive

Literature for pcr1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014