pcr1 (SPAC21E11.03c)


Gene Standard Namepcr1 Characterisation Statuspublished
Systematic IDSPAC21E11.03c Feature Typeprotein coding
Synonymsmts2 Name DescriptionM Twenty Six binding protein
Producttranscription factor Pcr1 Product Size171aa, 19.35 kDa
Genomic Location Chromosome I, 4253622-4252142 (1481nt); CDS:4252918-4252403 (516nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
double-stranded DNA binding141
Annotation ExtensionEvidenceWith/FromReference
protein binding872
Annotation ExtensionEvidenceWith/FromReference
recombination hotspot binding2
Annotation ExtensionEvidenceWith/FromReference
RNA binding582
Annotation ExtensionEvidenceWith/FromReference
RNA polymerase II core promoter proximal region sequence-specific DNA binding91
Annotation ExtensionEvidenceWith/FromReference
sequence-specific DNA binding130
Annotation ExtensionEvidenceWith/FromReference
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding90
Annotation ExtensionEvidenceWith/FromReference
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding32
GO Biological Process
Term NameCount
cellular response to nitrogen starvation21
Annotation ExtensionEvidenceWith/FromReference
positive regulation of mitotic G1 cell cycle arrest in response to nitrogen starvation2
Annotation ExtensionEvidenceWith/FromReference
positive regulation of transcription from RNA polymerase II promoter81
Annotation ExtensionEvidenceWith/FromReference
positive regulation of transcription from RNA polymerase II promoter in response to maltose2
Annotation ExtensionEvidenceWith/FromReference
protein localization to nucleus34
Annotation ExtensionEvidenceWith/FromReference
reciprocal meiotic recombination41
Annotation ExtensionEvidenceWith/FromReference
regulation of cAMP-mediated signaling8
Annotation ExtensionEvidenceWith/FromReference
regulation of cAMP-mediated signaling by regulation of transcription from RNA polymerase II promoter2
Annotation ExtensionEvidenceWith/FromReference
regulation of chromatin assembly14
Annotation ExtensionEvidenceWith/FromReference
regulation of chromatin disassembly2
Annotation ExtensionEvidenceWith/FromReference
regulation of histone acetylation7
Annotation ExtensionEvidenceWith/FromReference
regulation of transcription from RNA polymerase II promoter296
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
atf1-pcr1 complex2
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle271
Annotation ExtensionEvidenceWith/FromReference
nuclear chromatin359
Annotation ExtensionEvidenceWith/FromReference
nucleus2691
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased cell population growth at low temperaturepcr1Δ87
decreased cell population growth on glucose carbon sourcepcr1+ (wild type)320
decreased cell population growth on maltose carbon sourcepcr1Δ3
decreased mating efficiencypcr1T (N-term half of basic region deleted)272
pcr1Δ
decreased sporulation frequencypcr1-/pcr1- (homozygous diploid)103
resistance to caffeinepcr1Δ26
resistance to hydrogen peroxidepcr1Δ75
sensitive to cadmiumpcr1Δ249
sensitive to methylglyoxalpcr1Δ4
viable vegetative cell populationpcr1Δ3844

Cell Phenotype

Term NameGenotypesCount
abnormal microtubule cytoskeleton organization during vegetative growthpcr1+ (wild type)294
abnormal mitotic cell cycle phasepcr1Δ159
abnormal mitotic sister chromatid segregationpcr1+ (wild type)276
abolished RNA polymerase II core promoter proximal region sequence-specific DNA binding9
affecting atf1 and SO:0001843pcr1Δ
decreased cell cycle arrest in mitotic G1 phase in response to nitrogen starvationpcr1Δ14
decreased DNA binding16
affecting SO:0001843pcr1T (N-term half of basic region deleted)
decreased protein level during vegetative growth142
affecting ctt1pcr1Δ
decreased protein localization to M26 site2
affects localization of med7pcr1Δ
decreased protein localization to nucleus during cellular response to salt stress1
affects localization of sty1pcr1Δ
decreased protein localization to nucleus, with protein mislocalized to cytoplasm9
affects localization of atf1pcr1Δ
decreased RNA level during cellular response to hydrogen peroxide22
affecting tps1pcr1Δ
affecting ntp1pcr1Δ
decreased RNA level during cellular response to salt stress14
affecting tps1pcr1Δ
affecting gpd1pcr1Δ
affecting gpx1pcr1Δ
affecting ntp1pcr1Δ
affecting cta3pcr1Δ
decreased RNA level during nitrogen starvation43
affecting ste11pcr1Δ
decreased transcription during vegetative growth48
affecting agl1pcr1Δ
affecting fbp1pcr1Δ
normal cAMP levelpcr1Δ3
normal chromatin silencing at silent mating-type cassettepcr1Δ22
normal protein tyrosine phosphorylation during cellular response to osmotic stress4
affecting sty1pcr1Δ
viable elongated vegetative cellpcr1+ (wild type)320
pcr1Δ
viable vegetative cell with normal cell morphologypcr1Δ3100

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased mating efficiencypka1Δ, pcr1Δ

Cell Phenotype

Term NameGenotypes
decreased chromatin silencing at silent mating-type cassettesgf73Δ, pcr1Δ
ago1Δ, pcr1Δ
decreased RNA level during cellular response to salt stress
affecting gpd1pcr1Δ, tup11Δ, tup12Δ
affecting gpx1pcr1Δ, tup11Δ, tup12Δ
increased RNA level during cellular response to salt stress
affecting cta3pcr1Δ, tup11Δ, tup12Δ
RNA absent from cell during nitrogen starvation
affecting ste11pka1Δ, pcr1Δ
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in atf1 transcription factor, Atf-CREB family Atf1
FYPO affected by mutation in ctu2 cytosolic thiouridylase subunit Ctu2
FYPO affected by mutation in elp3 elongator complex subunit Elp3 (predicted)
FYPO affected by mutation in pka1 cAMP-dependent protein kinase catalytic subunit Pka1
FYPO affected by mutation in upf1 ATP-dependent RNA helicase Upf1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4253622..4252142
mRNA4253622..4252142
5' UTR4253622..4252919PMID:21511999
CDS4252918..4252403
3' UTR4252402..4252142
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00170 Pfam IPR004827 Basic-leucine zipper domain 12 70 5
SM00338 SMART IPR004827 Basic-leucine zipper domain 8 72 5
PS50217 Prosite Profiles IPR004827 Basic-leucine zipper domain 10 73 6
PS00036 Prosite Patterns IPR004827 Basic-leucine zipper domain 15 30 5
PTHR19304:SF27 HMMPANTHER 4 135 2
PTHR19304 HMMPANTHER 4 135 5
1.20.5.170 Gene3D Precursor fusion glycoprotein F0, Paramyxoviridae 5 74 7
SSF57959 SuperFamily 12 72 6
Coil ncoils Predicted coiled-coil protein (DUF2205) 35 69 1057

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000545bZIP (basic leucine zipper) transcription factor familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000545
PBO:0002433Atf-CREB subfamilyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0002433

Protein Properties

Ave. residue weight 113.15 Da
Charge 11.50
Codon Adaptation Index 0.46
Isoelectric point 10.53
Molecular weight 19.35 kDa
Number of residues 171
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelincreased during GO:0070301Western blot evidencePMID:23874237
present during GO:0072690Western blot evidencePMID:23874237
RNA levelincreased during GO:0070301Northern assay evidencePMID:23874237
increased in presence of cadmium sulfate expression microarray evidencePMID:12529438
increased during GO:0071470expression microarray evidencePMID:12529438
increased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
increased during GO:0034605expression microarray evidencePMID:12529438
increased during GO:0034599expression microarray evidencePMID:12529438

Quantitative Gene Expression

View graphical display of gene expression data for pcr1 (SPAC21E11.03c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2609.25during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
5039during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1798.96during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.5during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.69during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Subunit Composition
DescriptionQualifierReferenceCount
homomeric(2) PMID:795884953
heteromeric(2) PMID:795884943
Misc
DescriptionQualifierReferenceCount
deletion mutant expression profiling PMID:1825272177
Pathway
DescriptionQualifierReferenceCount
links stress-activated MAPK (Sty1) pathway to cAMP-dependent protein kinase (Pka1) pathway PMID:154481373
Taxonomic Conservation
DescriptionQualifierReferenceCount
orthologs cannot be distinguished124
conserved in fungi4606
conserved in eukaryotes4516
conserved in eukaryotes only2505
Orthologs

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC21E11.03c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
co-fractionates withatf1transcription factor, Atf-CREB family Atf1 Co-fractionationPMID:7958849
affinity captured byswi6HP1 family chromodomain protein Swi6 Affinity Capture-WesternPMID:15292231
affinity captured byspo5meiotic RNA-binding protein 1 Affinity Capture-RNAPMID:25023750
affinity captured byred1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
affinity captured byatf1transcription factor, Atf-CREB family Atf1 Affinity Capture-RNAPMID:22144913
affinity captured bymtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
binds to proteinspo5meiotic RNA-binding protein 1 Protein-RNAPMID:25023750
binds activation domain construct withatf1transcription factor, Atf-CREB family Atf1 Two-hybridPMID:26771498
binds activation domain construct withSPAC3F10.12ctranscription factor (predicted) Two-hybridPMID:26771498
interacts withatf1transcription factor, Atf-CREB family Atf1 PCAPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC21E11.03c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
rescued byste11transcription factor Ste11 Phenotypic SuppressionPMID:8552099
rescuespka1cAMP-dependent protein kinase catalytic subunit Pka1 Phenotypic SuppressionPMID:8552099
rescuestup12transcriptional corepressor Tup12 Phenotypic SuppressionPMID:11238405
rescuesspo5meiotic RNA-binding protein 1 Phenotypic SuppressionPMID:25023750
rescuestup11transcriptional corepressor Tup11 Phenotypic SuppressionPMID:11238405
positive genetic interaction withsre2membrane-tethered transcription factor Sre2 Positive GeneticPMID:21504829
phenotype enhanced bydcr1dicer Phenotypic EnhancementPMID:15218150
phenotype enhanced bydeb1transcription factor Deb1/Rdp1 Phenotypic EnhancementPMID:15218150
phenotype enhanced byago1argonaute Phenotypic EnhancementPMID:15218150
synthetically rescuessla1La protein homolog Synthetic RescuePMID:22160596
enhances phenotype ofatf1transcription factor, Atf-CREB family Atf1 Phenotypic EnhancementPMID:18375616
enhances phenotype ofers1RNA-silencing factor Ers1 Phenotypic EnhancementPMID:22733737
synthetic growth defect withchp1chromodomain protein Chp1 Synthetic Growth DefectPMID:15475954
synthetic growth defect withtas3RITS complex subunit 3 Synthetic Growth DefectPMID:15475954
synthetic growth defect withdcr1dicer Synthetic Growth DefectPMID:15475954
overexpression rescuesatf1transcription factor, Atf-CREB family Atf1 Dosage RescuePMID:10102365
External References
Database Identifier Description
NBRP SPAC21E11.03c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC21E11.03c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC21E11.03c BioGRID Interaction Datasets
Expression Viewer SPAC21E11.03c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC21E11.03c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC21E11.03c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC21E11.03c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC21E11.03c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC21E11.03c Transcriptome Viewer (Bähler Lab)
GEO SPAC21E11.03c GEO profiles
PInt SPAC21E11.03c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC21E11.03c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC21E11.03c Fission yeast phenotypic data & analysis
Cyclebase SPAC21E11.03c.1 Cell Cycle Data
SPD / RIKEN05/05A11Orfeome Localization Data
UniProtKB/SwissProtQ09926Transcription factor pcr1
ModBaseQ09926Database of comparative protein structure models
STRINGQ09926Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594500transcription factor Pcr1
RefSeq mRNANM_001019929972h- transcription factor Pcr1 (pcr1), mRNA
European Nucleotide ArchiveAAB46991.1ENA Protein Mapping
European Nucleotide ArchiveBAA09818.1ENA Protein Mapping
European Nucleotide ArchiveCAA91968.1ENA Protein Mapping
UniParcUPI000013144DUniProt Archive

Literature for pcr1

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016