pcr1 (SPAC21E11.03c)


Gene Standard Namepcr1 Characterisation Statuspublished
Systematic IDSPAC21E11.03c Feature Typeprotein coding
Synonymsmts2 Name DescriptionM Twenty Six binding protein
Producttranscription factor Pcr1 Product Size171aa, 19.35 kDa
Genomic Location Chromosome I, 4253622-4252142 (1481nt); CDS:4252918-4252403 (516nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
double-stranded DNA binding26
Annotation ExtensionEvidenceWith/FromReference
protein binding851
Annotation ExtensionEvidenceWith/FromReference
recombination hotspot binding2
Annotation ExtensionEvidenceWith/FromReference
RNA binding646
Annotation ExtensionEvidenceWith/FromReference
RNA polymerase II core promoter proximal region sequence-specific DNA binding90
Annotation ExtensionEvidenceWith/FromReference
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity91
Annotation ExtensionEvidenceWith/FromReference
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription29
Annotation ExtensionEvidenceWith/FromReference
sequence-specific DNA binding137
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cellular response to nitrogen starvation80
Annotation ExtensionEvidenceWith/FromReference
negative regulation of G1/S transition of mitotic cell cycle by positive regulation of transcription from RNA polymerase II promoter in response to nitrogen starvation2
Annotation ExtensionEvidenceWith/FromReference
positive regulation of transcription from RNA polymerase II promoter87
Annotation ExtensionEvidenceWith/FromReference
positive regulation of transcription, DNA-templated95
Annotation ExtensionEvidenceWith/FromReference
protein localization to nucleus28
Annotation ExtensionEvidenceWith/FromReference
reciprocal meiotic recombination42
Annotation ExtensionEvidenceWith/FromReference
regulation of cAMP-mediated signaling8
Annotation ExtensionEvidenceWith/FromReference
regulation of cAMP-mediated signaling by regulation of transcription from RNA polymerase II promoter2
Annotation ExtensionEvidenceWith/FromReference
regulation of chromatin assembly14
Annotation ExtensionEvidenceWith/FromReference
regulation of chromatin disassembly2
Annotation ExtensionEvidenceWith/FromReference
regulation of histone acetylation7
Annotation ExtensionEvidenceWith/FromReference
regulation of transcription from RNA polymerase II promoter289
Annotation ExtensionEvidenceWith/FromReference
transcription from RNA polymerase II promoter334
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
atf1-pcr1 complex2
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle258
Annotation ExtensionEvidenceWith/FromReference
nuclear chromatin307
Annotation ExtensionEvidenceWith/FromReference
nucleus2697
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growth on maltose carbon sourcepcr1ΔNull3
decreased mating efficiencypcr1ΔNull247
pcr1T (N-term half of basic region deleted)Not specified
resistance to caffeinepcr1ΔNull26
resistance to hydrogen peroxidepcr1ΔNull72
sensitive to cadmiumpcr1ΔNull246
sensitive to coldpcr1ΔNull45
sensitive to methylglyoxalpcr1ΔNull4
viable vegetative cell populationpcr1ΔNull3783

Cell Phenotype

Term NameAlleleExpressionCount
abnormal microtubule cytoskeleton organization during vegetative growthunnamed (overexpression)280
abnormal mitotic cell cycle arrest in response to nitrogen starvationpcr1ΔNull26
abnormal mitotic cell cycle phasepcr1ΔNull117
abolished RNA polymerase II core promoter proximal region sequence-specific DNA binding5
affecting atf1 and SO:0001843pcr1ΔNull
decreased DNA binding12
affecting SO:0001843pcr1T (N-term half of basic region deleted)Not specified
decreased protein localization to M26 site2
affecting med7pcr1ΔNull
decreased protein localization to nucleus during cellular response to salt stress1
affecting sty1pcr1ΔNull
decreased protein localization to nucleus, with protein mislocalized to cytoplasm3
affecting atf1pcr1ΔNull
decreased RNA level during cellular response to hydrogen peroxide20
affecting tps1pcr1ΔNull
affecting ntp1pcr1ΔNull
decreased RNA level during cellular response to salt stress12
affecting tps1pcr1ΔNull
affecting ntp1pcr1ΔNull
decreased RNA level during nitrogen starvation34
affecting ste11pcr1ΔNull
decreased sporulationpcr1-/pcr1- (homozygous diploid)Null65
decreased transcription during vegetative growth24
affecting agl1pcr1ΔNull
affecting fbp1pcr1ΔNull
normal cAMP levelpcr1ΔNull3
normal protein tyrosine phosphorylation during cellular response to osmotic stress4
affecting sty1pcr1ΔNull
viable elongated vegetative cellpcr1ΔNull231
viable vegetative cell with normal cell morphologypcr1ΔNull3092
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in atf1 transcription factor, Atf-CREB family Atf1 PMID:9135083
FYPO affected by mutation in ctu2 cytosolic thiouridylase subunit Ctu2 PMID:23874237
FYPO affected by mutation in elp3 elongator complex subunit Elp3 (predicted) PMID:23874237
FYPO affected by mutation in pka1 cAMP-dependent protein kinase catalytic subunit Pka1 PMID:8552099
FYPO affected by mutation in upf1 ATP-dependent RNA helicase Upf1 PMID:16914721
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
142536224252142

UTRs

Region Coordinates Reference
five_prime_UTR4253622..4252919PMID:21511999
intron4253113..4252919,4252141..4251918
exon4252918..4252403
mRNA4253622..4252142
three_prime_UTR4252402..4252142
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00170 Pfam IPR004827 Basic-leucine zipper domain 12 69 5
SM00338 SMART IPR004827 Basic-leucine zipper domain 8 72 5
PS50217 Prosite Profiles IPR004827 Basic-leucine zipper domain 10 73 6
PS00036 Prosite Patterns IPR004827 Basic-leucine zipper domain 15 30 5
PTHR19304 HMMPANTHER 5 171 2
1.20.5.170 Gene3D 5 74 7
SSF57959 SuperFamily 12 72 6
Coil ncoils Rabaptin coiled-coil domain 35 70 968

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000545bZIP (basic leucine zipper) transcription factor familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000545
PBO:0002433Atf-CREB subfamilyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0002433

Protein Properties

Ave. residue weight 113.15 Da
Charge 11.50
Isoelectric point 10.53
Molecular weight 19.35 kDa
Number of residues 171
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelpresent during GO:0072690Western blot evidencePMID:23874237
increased during GO:0070301Western blot evidencePMID:23874237
RNA levelincreased during GO:0034605expression microarray evidencePMID:12529438
increased during GO:0071470expression microarray evidencePMID:12529438
increased during GO:0034599expression microarray evidencePMID:12529438
increased in presence of cadmium sulfate expression microarray evidencePMID:12529438
increased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
increased during GO:0070301Northern assay evidencePMID:23874237

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5039during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2609.25during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1798.96during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.5during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.69during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Subunit Composition
DescriptionQualifierReferenceCount
homomeric(2) PMID:795884948
heteromeric(2) PMID:795884941
Misc
DescriptionQualifierReferenceCount
deletion mutant expression profiling PMID:1825272178
Pathway
DescriptionQualifierReferenceCount
links stress-activated MAPK (Sty1) pathway to cAMP-dependent protein kinase (Pka1) pathway PMID:154481373
Species Distribution
DescriptionQualifierReferenceCount
orthologs cannot be distinguished130
conserved in fungi4604
conserved in eukaryotes4516
conserved in eukaryotes only2503
Orthologs

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byatf1transcription factor, Atf-CREB family Atf1 Affinity Capture-WesternPMID:18375616
affinity captured byatf1transcription factor, Atf-CREB family Atf1 Affinity Capture-RNAPMID:22144913
co-fractionates withatf1transcription factor, Atf-CREB family Atf1 Co-fractionationPMID:7958849
affinity captured byatf1transcription factor, Atf-CREB family Atf1 Affinity Capture-WesternPMID:9135083
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
affinity captured bymtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
affinity captured byred1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
affinity captured byspo5meiotic RNA-binding protein 1 Affinity Capture-RNAPMID:25023750
binds to proteinspo5meiotic RNA-binding protein 1 Protein-RNAPMID:25023750
affinity captured byswi6HP1 family chromodomain protein Swi6 Affinity Capture-WesternPMID:15292231
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
phenotype enhanced byago1argonaute Phenotypic EnhancementPMID:15218150
enhances phenotype ofatf1transcription factor, Atf-CREB family Atf1 Phenotypic EnhancementPMID:18375616
overexpression rescuesatf1transcription factor, Atf-CREB family Atf1 Dosage RescuePMID:10102365
synthetic growth defect withchp1chromodomain protein Chp1 Synthetic Growth DefectPMID:15475954
synthetic growth defect withdcr1dicer Synthetic Growth DefectPMID:15475954
phenotype enhanced bydcr1dicer Phenotypic EnhancementPMID:15218150
phenotype enhanced bydeb1transcription factor Deb1/Rdp1 Phenotypic EnhancementPMID:15218150
enhances phenotype ofers1RNA-silencing factor Ers1 Phenotypic EnhancementPMID:22733737
rescuespka1cAMP-dependent protein kinase catalytic subunit Pka1 Phenotypic SuppressionPMID:8552099
synthetically rescuessla1La protein homolog Synthetic RescuePMID:22160596
rescuesspo5meiotic RNA-binding protein 1 Phenotypic SuppressionPMID:25023750
positive genetic interaction withsre2membrane-tethered transcription factor Sre2 Positive GeneticPMID:21504829
rescued byste11transcription factor Ste11 Phenotypic SuppressionPMID:8552099
synthetic growth defect withtas3RITS complex subunit 3 Synthetic Growth DefectPMID:15475954
rescuestup11transcriptional corepressor Tup11 Phenotypic SuppressionPMID:11238405
rescuestup12transcriptional corepressor Tup12 Phenotypic SuppressionPMID:11238405
External References
Database Identifier Description
NBRP SPAC21E11.03c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC21E11.03c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC21E11.03c BioGRID Interaction Datasets
Expression Viewer SPAC21E11.03c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC21E11.03c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC21E11.03c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC21E11.03c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC21E11.03c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC21E11.03c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC21E11.03c Cell Cycle Data
GEO SPAC21E11.03c GEO profiles
PInt SPAC21E11.03c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC21E11.03c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC21E11.03c Fission yeast phenotypic data & analysis
SPD / RIKEN05/05A11Orfeome Localization Data
UniProtKB/SwissProtQ09926Transcription factor pcr1
ModBaseQ09926Database of comparative protein structure models
STRINGQ09926Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594500transcription factor Pcr1
RefSeq mRNANM_001019929972h- transcription factor Pcr1 (pcr1), mRNA
European Nucleotide ArchiveAAB46991.1ENA Protein Mapping
European Nucleotide ArchiveBAA09818.1ENA Protein Mapping
European Nucleotide ArchiveCAA91968.1ENA Protein Mapping
UniParcUPI000013144DUniProt Archive

Literature for pcr1

Search: Europe PMC or PubMed

Release Version: PomBase:25_49 - 02 Feb 2015