atp2 (SPAC222.12c)


Gene Standard Nameatp2 Characterisation Statuspublished
Systematic IDSPAC222.12c Feature Typeprotein coding
Synonyms Name Description
ProductF1-ATPase beta subunit Atp2 Product Size525aa, 56.88 kDa
Genomic Location Chromosome I, 970412-968507 (1906nt); CDS:970360-968783 (1578nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ADP binding1
Annotation ExtensionEvidenceWith/FromReference
ATP binding551
Annotation ExtensionEvidenceWith/FromReference
ATPase activity238
Annotation ExtensionEvidenceWith/FromReference
proton-transporting ATP synthase activity, rotational mechanism17
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mitochondrial ATP synthesis coupled proton transport19
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
mitochondrial proton-transporting ATP synthase, catalytic core2
Annotation ExtensionEvidenceWith/FromReference
mitochondrion749
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
abolished cell population growth on glycerol carbon sourceB59168
abolished cell population growth on glycerol/ethanol carbon sourceatp2Δ17
decreased cell population growth on glucose carbon sourceR4-3 (Q170Y)393
atp2Δ
decreased cell population growth on glycerol carbon sourceR4-3 (Q170Y)80
decreased mating efficiencyatp2Δ276
sensitive to arsenicatp2Δ118
viable vegetative cell populationatp2Δ3862

Cell Phenotype

Term NameGenotypesCount
decreased ATPase activity9
affecting atp2R4-3 (Q170Y)
decreased cytochrome-c oxidase activityB5915
decreased hydrogen ion transporting ATP synthase activity, rotational mechanismB5912
decreased mitochondrial translationB5914
decreased ubiquinol-cytochrome-c reductase activityB5912
increased ADP binding1
affecting atp2R4-3 (Q170Y)
viable vegetative cell with normal cell morphologyatp2Δ3103
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in atp2 F1-ATPase beta subunit Atp2
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons970412..968507
mRNA970412..968507
5' UTR970412..970361PMID:21511999
CDS970360..968783
3' UTR968782..968507PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02874 Pfam IPR004100 ATPase, F1 complex alpha/beta subunit, N-terminal domain 57 126 4
PF00006 Pfam IPR000194 ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain 183 401 4
SM00382 SMART IPR003593 AAA+ ATPase domain 195 378 70
PS00152 Prosite Patterns IPR020003 ATPase, alpha/beta subunit, nucleotide-binding domain, active site 392 401 4
PTHR15184:SF44 HMMPANTHER 31 522 1
PTHR15184 HMMPANTHER 31 522 4
2ck3F03 Gene3D IPR024034 ATPase, F1 complex beta subunit/V1 complex, C-terminal 405 522 2
2.40.10.170 Gene3D Protein of unknown function DUF4438 47 128 1
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 129 403 288
SSF47917 SuperFamily IPR000793 ATPase, F1/V1/A1 complex, alpha/beta subunit, C-terminal 404 520 3
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 130 407 294
SSF50615 SuperFamily IPR004100 ATPase, F1 complex alpha/beta subunit, N-terminal domain 50 128 3
PIRSF039072 PIRSF 10 525 1
MF_01347 HAMAP IPR005722 ATPase, F1 complex, beta subunit 52 519 1
TIGR01039 TIGRFAM IPR005722 ATPase, F1 complex, beta subunit 54 519 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.34 Da
Charge -3.50
Codon Adaptation Index 0.58
Isoelectric point 5.78
Molecular weight 56.88 kDa
Number of residues 525
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS384, S386 2293
Annotation ExtensionEvidenceResidueReference
IDA S386 PMID:25720772
IDA S384 PMID:25720772
O-phosphorylated residueY391,S401 2461
Annotation ExtensionEvidenceResidueReference
IDA Y391,S401 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for atp2 (SPAC222.12c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
179631during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
186441during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
167659during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
168488during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
91271.13during GO:0072690PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
172599during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
97914.09during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
41during GO:0072690PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
5.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by S. cerevisiae ATP2 PMID:6228552
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3087
conserved in fungi4608
conserved in eukaryotes4516
conserved in bacteria1005
conserved in metazoa3498
conserved in vertebrates3473
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC222.12c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
affinity captured byslm9hira protein Slm9 Affinity Capture-MSPMID:20976105
affinity captured byclp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
affinity captured byfar8SIP/FAR complex striatin subunit, Far8/Csc3 Affinity Capture-MSPMID:22119525
affinity captured byimp1importin alpha Affinity Capture-MSPMID:25963819
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC222.12c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withapc10anaphase-promoting complex substrate recognition subunit Apc10 Negative GeneticPMID:22681890
negative genetic interaction withinp53inositol polyphosphate phosphatase Inp53 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withebs1EST1 family nonsense-mediated mRNA decay (NMD) pathway protein Ebs1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsrp1SR family protein, human SRFS2 ortholog Srp1 Negative GeneticPMID:22681890
negative genetic interaction withbzz1diacylglycerol binding protein Bzz1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withngg1SAGA complex subunit Ngg1/Ada3 Negative GeneticPMID:22681890
negative genetic interaction withask1DASH complex subunit Ask1 Negative GeneticPMID:22681890
negative genetic interaction withspc34DASH complex subunit Spc34 Negative GeneticPMID:22681890
negative genetic interaction withbdc1bromodomain containing protein 1, Bdc1 Negative GeneticPMID:22681890
negative genetic interaction withrdl2mitochondrial thiosulfate sulfurtransferase Rdl2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withdad2DASH complex subunit Dad2 Negative GeneticPMID:22681890
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
negative genetic interaction witherg5C-22 sterol desaturase Erg5 Negative GeneticPMID:22681890
negative genetic interaction withcaf4CCR4-Not complex subunit Caf4/Mdv1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsrb11cyclin CycC, Srb mediator subunit Srb11 Negative GeneticPMID:22681890
negative genetic interaction withssu72phosphoric ester hydrolase Ssu72 (predicted) Negative GeneticPMID:22681890
negative genetic interaction witharp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
negative genetic interaction witharp8Ino80 complex actin-like protein Arp8 Negative GeneticPMID:22681890
negative genetic interaction withcdm1DNA polymerase delta subunit Cdm1 Negative GeneticPMID:22681890
negative genetic interaction withcdr2serine/threonine protein kinase Cdr2 Negative GeneticPMID:22681890
positive genetic interaction withSPBP4H10.16cG-patch RNA-binding protein, involved in splicing (predicted) Positive GeneticPMID:22681890
positive genetic interaction withabo2ATPase with bromodomain protein (predicted) Positive GeneticPMID:22681890
positive genetic interaction withcdt2WD repeat protein Cdt2 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC222.12c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC222.12c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC222.12c BioGRID Interaction Datasets
Expression Viewer SPAC222.12c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC222.12c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC222.12c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC222.12c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC222.12c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC222.12c Transcriptome Viewer (Bähler Lab)
GEO SPAC222.12c GEO profiles
PInt SPAC222.12c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC222.12c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC222.12c Fission yeast phenotypic data & analysis
Cyclebase SPAC222.12c.1 Cell Cycle Data
IntEnz3.6.3.14Integrated relational Enzyme database
Rhea3.6.3.14Annotated reactions database
SPD / RIKEN25/25D07Orfeome Localization Data
UniProtKB/SwissProtP22068ATP synthase subunit beta, mitochondrial
ModBaseP22068Database of comparative protein structure models
STRINGP22068Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593151F1-ATPase beta subunit Atp2
RefSeq mRNANM_001018548972h- F1-ATPase beta subunit Atp2 (atp2), mRNA
European Nucleotide ArchiveCAB60704.1ENA Protein Mapping
MetaCycPWY-7219Adenosine ribonucleotides de novo biosynthesis
KEGG00190+3.6.3.14Oxidative phosphorylation
UniParcUPI0000126482UniProt Archive

Literature for atp2

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017