meu13 (SPAC222.15)


Gene Standard Namemeu13 Characterisation Statuspublished
Systematic IDSPAC222.15 Feature Typeprotein coding
SynonymsSPAC821.01 Name DescriptionMeiotic Expression Upregulated
ProductTat binding protein 1(TBP-1)-interacting protein (TBPIP) homolog (predicted) Product Size216aa, 24.67 kDa
Genomic Location Chromosome I, 977709-978637 (929nt); CDS:977709-978637 (929nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding887
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
DNA recombination122
Annotation ExtensionEvidenceWith/FromReference
synapsis10
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
horsetail nucleus14
Annotation ExtensionEvidenceWith/FromReference
nuclear chromatin365
Annotation ExtensionEvidenceWith/FromReference
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased spore germination frequencymeu13Δ69
viable vegetative cell populationmeu13Δ3850

Cell Phenotype

Term NameGenotypesCount
abnormal linear element morphologymeu13Δ6
abnormal nuclear separation after meiosismeu13Δ8
abnormal sporulation resulting in formation of ascus with fewer than four sporesmeu13Δ78
decreased gene conversion during vegetative growthmeu13Δ23
decreased homologous chromosome pairingmeu13Δ4
decreased intergenic meiotic recombinationmeu13Δ30
decreased intragenic meiotic recombinationmeu13Δ37
decreased protein degradation37
affecting mcp7meu13Δ
delayed onset of meiosis Imeu13Δ4
normal horsetail movementmeu13Δ5
normal meiotic sister chromatid cohesionmeu13Δ7
normal meiotic telomere clusteringmeu13Δ9
unequal homologous chromosome segregationmeu13Δ14
viable vegetative cell with normal cell morphologymeu13Δ3102

FYPO Multi-allele Phenotypes

Cell Phenotype

Term NameGenotypes
normal onset of meiosis Imcp7Δ, meu13Δ
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in mcp7 meiosis specific coiled-coil protein Mcp7
FYPO affected by mutation in red5 human ZC3H3 homolog
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons977709..977757, 977804..977857, 977905..978006, 978049..978189, 978333..978637
Intron977758..977803, 977858..977904, 978007..978048, 978190..978332
mRNA977709..978637
CDS977709..977757, 977804..977857, 977905..978006, 978049..978189, 978333..978637
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF07106 Pfam IPR010776 Tat binding protein 1-interacting 16 184 1
PTHR15938 HMMPANTHER IPR010776 Tat binding protein 1-interacting 8 209 1
1.10.10.10 Gene3D IPR011991 Winged helix-turn-helix DNA-binding domain 8 104 46
Coil ncoils Predicted coiled-coil protein (DUF2205) 82 109 1055
Coil ncoils Predicted coiled-coil protein (DUF2205) 133 153 1055

View domain organization at Pfam

Term IDTerm NameReferenceCount
SO:0001080coiled_coil107

Protein Properties

Ave. residue weight 114.22 Da
Charge 1.00
Codon Adaptation Index 0.42
Isoelectric point 7.41
Molecular weight 24.67 kDa
Number of residues 216
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelincreased during GO:0007128fusion protein localization evidencePMID:11447128
absent during GO:0007127Western blot evidencePMID:15210864
absent during GO:0007135Western blot evidencePMID:15210864
increased during GO:0051328Western blot evidencePMID:15210864
RNA levelabsent during GO:0072690Northern assay evidencePMID:11447128
increased during GO:0051321experimental evidencePMID:16303567
increased during GO:0051321Northern assay evidencePMID:11447128

Quantitative Gene Expression

View graphical display of gene expression data for meu13 (SPAC222.15)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.16during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.026during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
meiosis-specific splicing PMID:1589373214
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3093
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC222.15 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesmcp7meiosis specific coiled-coil protein Mcp7 Affinity Capture-WesternPMID:15210864
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC222.15 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetically rescued bymcp7meiosis specific coiled-coil protein Mcp7 Synthetic RescuePMID:15210864
enhances phenotype ofrec12meiotic recombination endonuclease Rec12 Phenotypic EnhancementPMID:11447128
enhances phenotype ofmcp7meiosis specific coiled-coil protein Mcp7 Phenotypic EnhancementPMID:15210864
negative genetic interaction withpnn1pinin ortholog, involved in splicing Pnn1 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withmug154conserved fungal protein Negative GeneticPMID:18818364
negative genetic interaction withsdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Negative GeneticPMID:18818364
negative genetic interaction withcdm1DNA polymerase delta subunit Cdm1 Negative GeneticPMID:18818364
negative genetic interaction withsgf29SAGA complex subunit Sgf29 Negative GeneticPMID:18818364
negative genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withtma23ribosome biogenesis protein Tma23 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withrsc4RSC complex subunit Rsc4 Negative GeneticPMID:18818364
negative genetic interaction withtip1CLIP170 family protein Tip1 Negative GeneticPMID:18818364
phenotype enhanced byrad17RFC related checkpoint protein Rad17 Phenotypic EnhancementPMID:12032093
phenotype enhanced byrad4BRCT domain protein Rad4 Phenotypic EnhancementPMID:14718568
phenotype enhanced bymek1Cds1/Rad53/Chk2 family protein kinase Mek1 Phenotypic EnhancementPMID:12032093
phenotype enhanced bycds1replication checkpoint kinase Cds1 Phenotypic EnhancementPMID:12032093
phenotype enhanced byrad3ATR checkpoint kinase Rad3 Phenotypic EnhancementPMID:12032093
positive genetic interaction withSPCC126.01cconserved fungal protein Positive GeneticPMID:18818364
positive genetic interaction withfig4inositol polyphosphate phosphatase Fig4 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withstp1protein tyrosine phosphatase Stp1 Positive GeneticPMID:18818364
positive genetic interaction withamk2AMP-activated protein kinase beta subunit Amk2 Positive GeneticPMID:18818364
positive genetic interaction withpus1tRNA pseudouridine synthase Lsp1 Positive GeneticPMID:18818364
positive genetic interaction withatg1801WD repeat protein involved in autophagy Atg18a Positive GeneticPMID:18818364
rescued bymek1Cds1/Rad53/Chk2 family protein kinase Mek1 Phenotypic SuppressionPMID:12482912
rescued byrec12meiotic recombination endonuclease Rec12 Phenotypic SuppressionPMID:12482912
rescued byrad4BRCT domain protein Rad4 Phenotypic SuppressionPMID:14718568
External References
Database Identifier Description
NBRP SPAC222.15 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC222.15 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC222.15 BioGRID Interaction Datasets
Expression Viewer SPAC222.15 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC222.15 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC222.15 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC222.15 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC222.15 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC222.15 Transcriptome Viewer (Bähler Lab)
GEO SPAC222.15 GEO profiles
PInt SPAC222.15 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC222.15 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC222.15 Fission yeast phenotypic data & analysis
Cyclebase SPAC222.15.1 Cell Cycle Data
SPD / RIKEN13/13A01Orfeome Localization Data
UniProtKB/SwissProtQ9HGK2Homologous-pairing protein 2
ModBaseQ9HGK2Database of comparative protein structure models
STRINGQ9HGK2Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_001018191homologous-pairing protein 2
RefSeq mRNANM_001018551972h- homologous-pairing protein 2 (meu13), mRNA
European Nucleotide ArchiveBAB17055.1ENA Protein Mapping
European Nucleotide ArchiveCAD33796.1ENA Protein Mapping
UniParcUPI000012EE93UniProt Archive

Literature for meu13

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016