gim3 (SPAC227.05)


Gene Standard Namegim3 Characterisation Statusbiological_role_inferred
Systematic IDSPAC227.05 Feature Typeprotein coding
Synonyms Name Description
Productprefoldin subunit 4, Gim3 (predicted) Product Size123aa, 14.19 kDa
Genomic Location Chromosome I, 505902-506355 (454nt); CDS:505984-506355 (372nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
unfolded protein binding41
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
protein folding103
Annotation ExtensionEvidenceWith/FromReference
tubulin complex assembly20
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
nucleus2695
Annotation ExtensionEvidenceWith/FromReference
prefoldin complex6
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
viable vegetative cell populationgim3ΔNull3795

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologygim3ΔNull3093
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons505902..506355
mRNA505902..506355
5' UTR505902..505983PMID:18641648
5' UTR505902..505983PMID:20118936
CDS505984..506355
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01920 Pfam IPR002777 Prefoldin beta-like 11 119 4
PTHR21100 HMMPANTHER IPR016661 Prefoldin, subunit 4 1 123 1
PTHR21100:SF9 HMMPANTHER 1 123 1
3.30.1460.10 Gene3D 5 48 1
SSF46579 SuperFamily IPR009053 Prefoldin 12 113 6
Coil ncoils Rabaptin coiled-coil domain 77 105 968
Coil ncoils Rabaptin coiled-coil domain 31 55 968
PIRSF016477 PIRSF IPR016661 Prefoldin, subunit 4 1 123 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 115.36 Da
Charge -16.00
Isoelectric point 4.02
Molecular weight 14.19 kDa
Number of residues 123
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
14295during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
15682during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
4928.48during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
14159during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1563.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7.9during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withago1argonaute Negative GeneticPMID:18818364
negative genetic interaction withago1argonaute Negative GeneticPMID:22681890
negative genetic interaction withair1zinc knuckle TRAMP complex subunit Air1 Negative GeneticPMID:18818364
negative genetic interaction withair1zinc knuckle TRAMP complex subunit Air1 Negative GeneticPMID:22681890
negative genetic interaction withalp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
phenotype enhanced byalp13MRG family Clr6 histone deacetylase complex subunit Alp13 Phenotypic EnhancementPMID:19547744
negative genetic interaction withalp14TOG/XMAP14 family protein Alp14 Negative GeneticPMID:18818364
negative genetic interaction withalp31tubulin specific chaperone cofactor A, Alp31 Negative GeneticPMID:18818364
negative genetic interaction withase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:18818364
negative genetic interaction withase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:22681890
positive genetic interaction withatp15F0-ATPase epsilon subunit (predicted) Positive GeneticPMID:22681890
negative genetic interaction withbdc1bromodomain containing protein 1, Bdc1 Negative GeneticPMID:18818364
negative genetic interaction withbdc1bromodomain containing protein 1, Bdc1 Negative GeneticPMID:22681890
positive genetic interaction withcay1cactin, spliceosome complex subunit (predicted) Positive GeneticPMID:18818364
positive genetic interaction withcdt2WD repeat protein Cdt2 Positive GeneticPMID:22681890
negative genetic interaction withcho1phosphatidyl-N-methylethanolamine N-methyltransferase (predicted) Negative GeneticPMID:18818364
negative genetic interaction withcrn1actin binding protein, coronin Crn1 Negative GeneticPMID:18818364
negative genetic interaction withdad2DASH complex subunit Dad2 Negative GeneticPMID:18818364
negative genetic interaction withdad2DASH complex subunit Dad2 Negative GeneticPMID:22681890
negative genetic interaction withdad3DASH complex subunit Dad3 Negative GeneticPMID:22681890
negative genetic interaction withdam1DASH complex subunit Dam1 Negative GeneticPMID:22681890
positive genetic interaction withdcd1deoxycytidylate deaminase (predicted) Positive GeneticPMID:22681890
negative genetic interaction withdcr1dicer Negative GeneticPMID:18818364
negative genetic interaction withdcr1dicer Negative GeneticPMID:22681890
negative genetic interaction withduo1DASH complex subunit Duo1 Negative GeneticPMID:22681890
positive genetic interaction witheif21translation initiation factor eIF2A (predicted) Positive GeneticPMID:18818364
positive genetic interaction withelp2elongator complex subunit Elp2 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withemc1ER membrane protein complex subunit Emc1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction witherg28Erg28 protein (predicted) Positive GeneticPMID:22681890
negative genetic interaction withers1RNA-silencing factor Ers1 Negative GeneticPMID:18818364
negative genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:18818364
negative genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
positive genetic interaction withgcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Positive GeneticPMID:22681890
positive genetic interaction withgmh5alpha-1,2-galactosyltransferase (predicted) Positive GeneticPMID:18818364
negative genetic interaction withhap2HMG box protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhhp2serine/threonine protein kinase Hhp2 Negative GeneticPMID:18818364
negative genetic interaction withhhp2serine/threonine protein kinase Hhp2 Negative GeneticPMID:22681890
positive genetic interaction withhmt2sulfide-quinone oxidoreductase Positive GeneticPMID:22681890
positive genetic interaction withhrq1RecQ type DNA helicase Hrq1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withhrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:18818364
negative genetic interaction withivn1CDC50 domain protein, implicated in signal transduction (predicted) Negative GeneticPMID:22681890
positive genetic interaction withlub1WD repeat protein Lub1 Positive GeneticPMID:18818364
positive genetic interaction withmac1membrane anchored protein Mac1 Positive GeneticPMID:22681890
negative genetic interaction withmga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Negative GeneticPMID:18818364
positive genetic interaction withmph1dual specificity protein kinase Mph1 Positive GeneticPMID:22681890
negative genetic interaction withmsd1mitotic-spindle disanchored Msd1 Negative GeneticPMID:22681890
positive genetic interaction withmug154conserved fungal protein Positive GeneticPMID:22681890
positive genetic interaction withnrm1MBF complex corepressor Nrm1 Positive GeneticPMID:22681890
negative genetic interaction withpab2poly(A) binding protein Pab2 Negative GeneticPMID:22681890
negative genetic interaction withpdh1DUF1751 family protein Negative GeneticPMID:22681890
negative genetic interaction withpds5mitotic cohesin-associated protein Pds5 Negative GeneticPMID:18818364
positive genetic interaction withpof9F-box protein Pof9 Positive GeneticPMID:22681890
positive genetic interaction withres1MBF transcription factor complex subunit Res1 Positive GeneticPMID:22681890
negative genetic interaction withrga3Rho-type GTPase activating protein Rga3 Negative GeneticPMID:22681890
positive genetic interaction withrpa12DNA-directed RNA polymerase complex I subunit Rpa12 Positive GeneticPMID:22681890
positive genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withrpl250260S ribosomal protein L25 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withrpl70260S ribosomal protein L7 Positive GeneticPMID:22681890
positive genetic interaction withrps170140S ribosomal protein S17 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withrps40140S ribosomal protein S4 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withrsc1RSC complex subunit Rsc1 Negative GeneticPMID:18818364
negative genetic interaction withrsv2transcription factor Rsv2 Negative GeneticPMID:22681890
positive genetic interaction withryh1GTPase Ryh1 Positive GeneticPMID:18818364
negative genetic interaction withsap114U2 snRNP subunit Sap114 Negative GeneticPMID:22681890
positive genetic interaction withsck2serine/threonine protein kinase Sck2 Positive GeneticPMID:24463365
positive genetic interaction withsdc1Dpy-30 domain protein Sdc1 Positive GeneticPMID:18818364
positive genetic interaction withsfp1transcription factor Sfp1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withSPAC1039.02phosphoprotein phosphatase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC139.01cnuclease, XP-G family (predicted) Negative GeneticPMID:18818364
positive genetic interaction withSPAC1486.03cRNA-binding splicing factor (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPAC1805.14Schizosaccharomyces specific protein Positive GeneticPMID:22681890
positive genetic interaction withSPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
synthetic growth defect withSPAC3H1.05CAAX prenyl protease (predicted) Synthetic Growth DefectPMID:18931302
positive genetic interaction withSPAP8A3.07cphospho-2-dehydro-3-deoxyheptonate aldolase (predicted) Positive GeneticPMID:22681890
negative genetic interaction withSPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:18818364
positive genetic interaction withSPBC16H5.13WD repeat protein, human WDR7 ortholog Positive GeneticPMID:22681890
positive genetic interaction withSPBC32F12.12cGolgi membrane protein involved in vesicle-mediated transport (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPBC336.13cmitochondrial inner membrane peptidase complex catalytic subunit 2 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withSPBC725.01aspartate aminotransferase (predicted) Negative GeneticPMID:22681890
positive genetic interaction withSPBP35G2.14RNA-binding protein Positive GeneticPMID:22681890
negative genetic interaction withspc19DASH complex subunit Spc19 Negative GeneticPMID:22681890
negative genetic interaction withspc2signal peptidase subunit Spc2 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withSPCC1450.12PXA domain protein Positive GeneticPMID:22681890
positive genetic interaction withSPCC162.11curidine kinase/uracil phosphoribosyltransferase (predicted) Positive GeneticPMID:18818364
positive genetic interaction withSPCC162.11curidine kinase/uracil phosphoribosyltransferase (predicted) Positive GeneticPMID:22681890
negative genetic interaction withsqs1R3H and G-patch domain Sqs1(predicted) Negative GeneticPMID:22681890
positive genetic interaction withsrb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Positive GeneticPMID:22681890
negative genetic interaction withssa1heat shock protein Ssa1 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withssp2AMP-activated protein serine/threonine kinase alpha subunit Ssp2 Positive GeneticPMID:22681890
positive genetic interaction withsst4sorting receptor for ubiquitinated membrane proteins, ESCRT 0 complex subunit Sst4 Positive GeneticPMID:22681890
positive genetic interaction withsts5RNB-like protein Positive GeneticPMID:22681890
positive genetic interaction withswd1Set1C complex subunit Swd1 Positive GeneticPMID:22681890
positive genetic interaction withtea1cell end marker Tea1 Positive GeneticPMID:22681890
positive genetic interaction withtom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withwtf16wtf element Wtf16 Negative GeneticPMID:22681890
positive genetic interaction withypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC227.05 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC227.05 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC227.05 BioGRID Interaction Datasets
Expression Viewer SPAC227.05 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC227.05 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC227.05 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC227.05 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC227.05 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC227.05 Transcriptome Viewer (Bähler Lab)
GEO SPAC227.05 GEO profiles
PInt SPAC227.05 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC227.05 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC227.05 Fission yeast phenotypic data & analysis
Cyclebase SPAC227.05.1 Cell Cycle Data
SPD / RIKEN02/02F01Orfeome Localization Data
UniProtKB/SwissProtQ9UTD4Probable prefoldin subunit 4
ModBaseQ9UTD4Database of comparative protein structure models
STRINGQ9UTD4Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_592959prefoldin subunit 4 (predicted)
RefSeq mRNANM_001018359972h- prefoldin subunit 4 (predicted) (SPAC227.05), mRNA
European Nucleotide ArchiveCAB61454.1ENA Protein Mapping
UniParcUPI0000131729UniProt Archive

Literature for gim3

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015