SPAC227.05


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPAC227.05 Feature Typeprotein coding
Synonyms Name Description
Productprefoldin subunit 4 (predicted) Product Size123aa, 14.19 kDa
Genomic Location Chromosome I, 505902-506355 (454nt); CDS:505984-506355 (372nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0051082unfolded protein bindingIEAIPR002777GO_REF:000000242
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006457protein foldingIEAIPR002777GO_REF:0000002111
GO:0007021tubulin complex assemblyISSUniProtKB:P53900GO_REF:000000120
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722316
GO:0005634nucleusIDAPMID:168233722740
GO:0016272prefoldin complexISSUniProtKB:P53900GO_REF:00000016
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopySPAC227.05ΔNullPMID:204732893730
MicroscopySPAC227.05ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001MicroscopySPAC227.05ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1505902506355

UTRs

Region Coordinates Reference
five_prime_UTR505902..505983PMID:18641648
five_prime_UTR505902..505983PMID:20118936
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01920 Pfam IPR002777 Prefoldin beta-like 11 119 4
PTHR21100 HMMPANTHER IPR016661 Prefoldin, subunit 4 1 123 1
3.30.1460.10 Gene3D 5 48 1
SSF46579 SuperFamily IPR009053 Prefoldin 12 113 6
Coil ncoils Rabaptin coiled-coil domain 77 105 968
Coil ncoils Rabaptin coiled-coil domain 31 55 968
PIRSF016477 PIRSF IPR016661 Prefoldin, subunit 4 1 123 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 115.36 Da
Charge -16.00
Isoelectric point 4.02
Molecular weight 14.19 kDa
Number of residues 123
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
14295during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
15682during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
4928.48during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
14159during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
1563.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
7.9during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
1.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
duo1DASH complex subunit Duo1 Negative GeneticPMID:22681890
cdt2WD repeat protein Cdt2 Positive GeneticPMID:22681890
ase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
lub1WD repeat protein Lub1 Positive GeneticPMID:18818364
mph1dual specificity protein kinase Mph1 Positive GeneticPMID:22681890
bdc1bromodomain containing protein 1, Bdc1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
ago1argonaute Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
pof9F-box protein Pof9 Positive GeneticPMID:22681890
SPBC725.01aspartate aminotransferase (predicted) Negative GeneticPMID:22681890
pds5cohesin-associated protein Pds5 Negative GeneticPMID:18818364
pab2poly(A) binding protein Pab2 Negative GeneticPMID:22681890
atp15F0-ATPase epsilon subunit (predicted) Positive GeneticPMID:22681890
mga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Negative GeneticPMID:18818364
rpa12DNA-directed RNA polymerase complex I subunit Rpa12 Positive GeneticPMID:22681890
rps40140S ribosomal protein S4 (predicted) Positive GeneticPMID:22681890
rps170140S ribosomal protein S17 (predicted) Positive GeneticPMID:22681890
SPAC139.01cnuclease, XP-G family (predicted) Negative GeneticPMID:18818364
swd1Set1C complex subunit Swd1 Positive GeneticPMID:22681890
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Positive GeneticPMID:22681890
cho1phosphatidyl-N-methylethanolamine N-methyltransferase (predicted) Negative GeneticPMID:18818364
spc2signal peptidase subunit Spc2 (predicted) Negative GeneticPMID:22681890
SPCC16C4.20cHMG box protein (predicted) Negative GeneticPMID:22681890
res1MBF transcription factor complex subunit Res1 Positive GeneticPMID:22681890
SPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:18818364
Positive GeneticPMID:22681890
sfp1transcription factor Sfp1 (predicted) Positive GeneticPMID:22681890
mac1membrane anchored protein Mac1 Positive GeneticPMID:22681890
alp14TOG ortholog Alp14 Negative GeneticPMID:18818364
SPBC336.13cmitochondrial inner membrane peptidase complex catalytic subunit 2 (predicted) Positive GeneticPMID:22681890
gmh5alpha-1,2-galactosyltransferase (predicted) Positive GeneticPMID:18818364
rpl70260S ribosomal protein L7 Positive GeneticPMID:22681890
ers1RNA-silencing factor Ers1 Negative GeneticPMID:18818364
dcd1deoxycytidylate deaminase (predicted) Positive GeneticPMID:22681890
hrq1RecQ type DNA helicase Hrq1 (predicted) Positive GeneticPMID:22681890
SPBC4B4.04translation initiation factor eIF2A (predicted) Positive GeneticPMID:18818364
cay1cactin, spliceosome complex subunit (predicted) Positive GeneticPMID:18818364
alp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
Phenotypic EnhancementPMID:19547744
nrm1MBF complex corepressor Nrm1 Positive GeneticPMID:22681890
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Positive GeneticPMID:22681890
dad2DASH complex subunit Dad2 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
crn1actin binding protein, coronin Crn1 Negative GeneticPMID:18818364
SPCC1450.12PXA domain protein Positive GeneticPMID:22681890
SPAC3H1.05CAAX prenyl protease (predicted) Synthetic Growth DefectPMID:18931302
mug154conserved fungal protein Positive GeneticPMID:22681890
rpl250260S ribosomal protein L25 (predicted) Positive GeneticPMID:22681890
sck2serine/threonine protein kinase Sck2 Positive GeneticPMID:24463365
SPAC1039.02phosphoprotein phosphatase (predicted) Negative GeneticPMID:22681890
rsv2transcription factor Rsv2 Negative GeneticPMID:22681890
ryh1GTPase Ryh1 Positive GeneticPMID:18818364
tom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Positive GeneticPMID:22681890
rpl160160S ribosomal protein L13/L16 (predicted) Positive GeneticPMID:18818364
Positive GeneticPMID:22681890
dad3DASH complex subunit Dad3 Negative GeneticPMID:22681890
sts5RNB-like protein Positive GeneticPMID:22681890
wtf16wtf element Wtf16 Negative GeneticPMID:22681890
msd1mitotic-spindle disanchored Msd1 Negative GeneticPMID:22681890
erg28Erg28 protein (predicted) Positive GeneticPMID:22681890
SPAC1805.14sequence orphan Positive GeneticPMID:22681890
ssp2serine/threonine protein kinase Ssp2 Positive GeneticPMID:22681890
alp31tubulin specific chaperone cofactor A, Alp31 Negative GeneticPMID:18818364
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:18818364
SPBC30B4.02cR3H and G-patch domain, implicated in splicing (predicted) Negative GeneticPMID:22681890
hmt2sulfide-quinone oxidoreductase Positive GeneticPMID:22681890
air1zinc knuckle TRAMP complex subunit Air1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
ssa1heat shock protein Ssa1 (predicted) Negative GeneticPMID:22681890
emc1ER membrane protein complex subunit Emc1 (predicted) Positive GeneticPMID:22681890
rga3Rho-type GTPase activating protein Rga3 Negative GeneticPMID:22681890
sst4sorting receptor for ubiquitinated membrane proteins, ESCRT 0 complex subunit Sst4 Positive GeneticPMID:22681890
SPBP35G2.14RNA-binding protein Positive GeneticPMID:22681890
pdh1DUF1751 family protein Negative GeneticPMID:22681890
SPCC162.11curidine kinase/uracil phosphoribosyltransferase (predicted) Positive GeneticPMID:18818364
Positive GeneticPMID:22681890
SPBC32F12.12cGolgi membrane protein involved in vesicle-mediated transport (predicted) Positive GeneticPMID:22681890
ivn1CDC50 domain protein, implicated in signal transduction (predicted) Negative GeneticPMID:22681890
dcr1dicer Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
SPAC1486.03cRNA-binding splicing factor (predicted) Positive GeneticPMID:22681890
sdc1Dpy-30 domain protein Sdc1 Positive GeneticPMID:18818364
elp2elongator complex subunit Elp2 (predicted) Positive GeneticPMID:22681890
sap114U2 snRNP subunit Sap114 Negative GeneticPMID:22681890
rsc1RSC complex subunit Rsc1 Negative GeneticPMID:18818364
tea1cell end marker Tea1 Positive GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
SPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
SPAP8A3.07cphospho-2-dehydro-3-deoxyheptonate aldolase (predicted) Positive GeneticPMID:22681890
hhp2serine/threonine protein kinase Hhp2 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
dam1DASH complex subunit Dam1 Negative GeneticPMID:22681890
spc19DASH complex subunit Spc19 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC227.05 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC227.05 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC227.05 BioGRID Interaction Datasets
Expression Viewer SPAC227.05 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC227.05 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC227.05 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC227.05 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC227.05 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC227.05 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC227.05 Cell Cycle Data
GEO SPAC227.05 GEO profiles
PInt SPAC227.05 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC227.05 Peptides identified in tandem mass spectrometry proteomics experiments
WikiGene2542066prefoldin subunit 4 (predicted)
EntrezGene2542066prefoldin subunit 4 (predicted)
SPD / RIKEN02/02F01Orfeome Localization Data
UniProtKB/SwissProtQ9UTD4Probable prefoldin subunit 4
ModBaseQ9UTD4Database of comparative protein structure models
STRINGQ9UTD4Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_592959prefoldin subunit 4 (predicted)
RefSeq mRNANM_001018359972h- prefoldin subunit 4 (predicted) (SPAC227.05), mRNA
European Nucleotide ArchiveCAB61454ENA Protein Mapping
European Nucleotide ArchiveCAB61454.1ENA Protein Mapping
UniParcUPI0000131729UniProt Archive

Literature for SPAC227.05

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014