ept1 (SPAC22A12.10)


Gene Standard Nameept1 Characterisation Statusbiological role inferred
Systematic IDSPAC22A12.10 Feature Typeprotein coding
Synonymscpt1 Name Description
Productdiacylglycerol cholinephosphotransferase/ diacylglycerol ethanolaminesphotransferase Ept1 (predicted) Product Size386aa, 44.08 kDa
Genomic Location Chromosome I, 1172209-1176801 (4593nt); CDS:1174571-1175781 (1211nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
diacylglycerol cholinephosphotransferase activity1
Annotation ExtensionEvidenceWith/FromReference
ethanolaminephosphotransferase activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
phosphatidylcholine biosynthetic process9
Annotation ExtensionEvidenceWith/FromReference
phosphatidylethanolamine biosynthetic process9
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum596
Annotation ExtensionEvidenceWith/FromReference
endoplasmic reticulum membrane201
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane962
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationept1Δ3844

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologyept1Δ3100
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1172209..1174707, 1174758..1176801
Intron1174708..1174757
mRNA1172209..1176801
5' UTR1172209..1174570PMID:21511999
CDS1174571..1174707, 1174758..1175781
3' UTR1175782..1176801PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01066 Pfam IPR000462 CDP-alcohol phosphatidyltransferase 45 121 4
TMhelix TMHMM 313 335 953
TMhelix TMHMM 345 367 953
TMhelix TMHMM 252 274 953
TMhelix TMHMM 284 306 953
TMhelix TMHMM 135 157 953
TMhelix TMHMM 169 191 953
TMhelix TMHMM 217 239 953
TMhelix TMHMM 46 68 953
PS00379 Prosite Patterns IPR000462 CDP-alcohol phosphatidyltransferase 99 121 4
PTHR10414 HMMPANTHER IPR014472 Choline/ethanolamine phosphotransferase 3 386 1
PTHR10414:SF37 HMMPANTHER 3 386 1
PIRSF015665 PIRSF IPR014472 Choline/ethanolamine phosphotransferase 1 386 1

View domain organization at Pfam

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 114.21 Da
Charge 3.50
Codon Adaptation Index 0.47
Isoelectric point 7.26
Molecular weight 44.08 kDa
Number of residues 386
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for ept1 (SPAC22A12.10)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
79737during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
81976during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
79849during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
83180during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
10326.09during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
80518during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
6634.58during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.4during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC22A12.10 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withman1LEM domain protein Man1, Sad1 interacting factor Two-hybridPMID:26771498
External References
Database Identifier Description
NBRP SPAC22A12.10 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC22A12.10 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC22A12.10 BioGRID Interaction Datasets
Expression Viewer SPAC22A12.10 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC22A12.10 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC22A12.10 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC22A12.10 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC22A12.10 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC22A12.10 Transcriptome Viewer (Bähler Lab)
GEO SPAC22A12.10 GEO profiles
PInt SPAC22A12.10 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC22A12.10 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC22A12.10 Fission yeast phenotypic data & analysis
Cyclebase SPAC22A12.10.1 Cell Cycle Data
IntEnz2.7.8.2Integrated relational Enzyme database
Rhea2.7.8.2Annotated reactions database
IntEnz2.7.8.1Integrated relational Enzyme database
Rhea2.7.8.1Annotated reactions database
SPD / RIKEN22/22B04Orfeome Localization Data
UniProtKB/SwissProtO13901Uncharacterized CDP-alcohol phosphatidyltransferase class-I family protein C22A12.10
ModBaseO13901Database of comparative protein structure models
STRINGO13901Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593240CDP-alcohol phosphatidyltransferase class-I family protein
RefSeq mRNANM_001018637972h- CDP-alcohol phosphatidyltransferase class-I family protein (SPAC22A12.10), mRNA
European Nucleotide ArchiveCAB16580.1ENA Protein Mapping
UniParcUPI000006982FUniProt Archive

Literature for ept1

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016