bip1 (SPAC22A12.15c)


Gene Standard Namebip1 Characterisation Statuspublished
Systematic IDSPAC22A12.15c Feature Typeprotein coding
Synonymsbip Name Description
ProductER heat shock protein BiP Product Size663aa, 73.23 kDa
Genomic Location Chromosome I, 1185497-1182693 (2805nt); CDS:1185303-1183312 (1992nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
ATPase activity236
Annotation ExtensionEvidenceWith/FromReference
protein binding859
Annotation ExtensionEvidenceWith/FromReference
unfolded protein binding41
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
'de novo' posttranslational protein folding2
Annotation ExtensionEvidenceWith/FromReference
cellular response to unfolded protein15
Annotation ExtensionEvidenceWith/FromReference
ER-associated ubiquitin-dependent protein catabolic process28
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum596
Annotation ExtensionEvidenceWith/FromReference
endoplasmic reticulum lumen13
Annotation ExtensionEvidenceWith/FromReference
Golgi apparatus357
Annotation ExtensionEvidenceWith/FromReference
nuclear membrane32
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationbip1ΔNull1438
bip1Δ::ura4+Null

Cell Phenotype

Term NameAlleleExpressionCount
inviable sporebip1ΔNull472
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in alg6 glucosyltransferase Alg6
FYPO affected by mutation in cnx1 calnexin Cnx1
FYPO affected by mutation in gpt1 UDP-glucose-glycoprotein glucosyltransferase Gpt1
FYPO affected by mutation in ire1 serine/threonine protein kinase, sensor for unfolded proteins in the ER Ire1
FYPO affected by mutation in srp54 signal recognition particle subunit Srp54
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1185497..1182693
mRNA1185497..1182693
5' UTR1185497..1185304PMID:21511999
CDS1185303..1183312
3' UTR1183311..1182693PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00012 Pfam IPR013126 Heat shock protein 70 family 37 641 8
TMhelix TMHMM 7 24 959
PS00329 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 227 240 6
PS00297 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 40 47 5
PS01036 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 364 378 7
PTHR19375 HMMPANTHER 35 660 8
3.30.30.30 Gene3D 86 146 6
3.30.420.40 Gene3D 37 85 24
3.30.420.40 Gene3D 154 231 24
1.20.1270.10 Gene3D IPR029048 Heat shock protein 70kD, C-terminal domain 546 648 6
2.60.34.10 Gene3D IPR029047 Heat shock protein 70kD, peptide-binding domain 419 545 7
3.30.420.40 Gene3D 232 260 24
3.30.420.40 Gene3D 346 387 24
3.90.640.10 Gene3D 261 341 16
SSF100934 SuperFamily IPR029048 Heat shock protein 70kD, C-terminal domain 565 646 7
SSF53067 SuperFamily 221 411 25
SSF53067 SuperFamily 35 218 25
SSF100920 SuperFamily IPR029047 Heat shock protein 70kD, peptide-binding domain 414 571 7
SignalP-noTM signalp 1 24 212
Coil ncoils Rabaptin coiled-coil domain 543 564 968
Coil ncoils Rabaptin coiled-coil domain 290 311 968
PR00301 PRINTS IPR013126 Heat shock protein 70 family 86 94 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 36 49 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 361 377 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 417 436 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 173 193 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 233 243 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 392 412 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 64 76 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 498 514 8

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0000418signal_peptide279
SO:0001806ER_retention_signal3
residue A660-L663PMID:1373379

Protein Properties

Ave. residue weight 110.45 Da
Charge -28.00
Isoelectric point 4.57
Molecular weight 73.23 kDa
Number of residues 663
Modifications

Protein Modifications

Term NameResidueCount
glycosylated residue 36
Annotation ExtensionEvidenceResidueReference
NAS PMID:8227200
IDA PMID:1373379
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0034605Northern assay evidencePMID:1373379

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
515066during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
505443during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
515101during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
539781during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
507789during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
123452.29during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
35320.11during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
41during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
4.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bycnx1calnexin Cnx1 Affinity Capture-WesternPMID:9860839
affinity captured bycnx1calnexin Cnx1 Affinity Capture-WesternPMID:17230581
affinity captured bynak1PAK-related kinase Nak1 Affinity Capture-MSPMID:23462181
affinity captured bysog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
External References
Database Identifier Description
NBRP SPAC22A12.15c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC22A12.15c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC22A12.15c BioGRID Interaction Datasets
Expression Viewer SPAC22A12.15c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC22A12.15c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC22A12.15c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC22A12.15c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC22A12.15c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC22A12.15c Transcriptome Viewer (Bähler Lab)
GEO SPAC22A12.15c GEO profiles
PInt SPAC22A12.15c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC22A12.15c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC22A12.15c Fission yeast phenotypic data & analysis
Cyclebase SPAC22A12.15c.1 Cell Cycle Data
SPD / RIKEN27/27C11Orfeome Localization Data
UniProtKB/SwissProtP3660478 kDa glucose-regulated protein homolog
ModBaseP36604Database of comparative protein structure models
STRINGP36604Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593245ER heat shock protein BiP
RefSeq mRNANM_001018642972h- ER heat shock protein BiP (bip1), mRNA
European Nucleotide ArchiveCAA45762.1ENA Protein Mapping
European Nucleotide ArchiveCAB16585.1ENA Protein Mapping
UniParcUPI000012BA5CUniProt Archive

Literature for bip1

Search: Europe PMC or PubMed

Release Version: PomBase:26_53 - 27 May 2015