bip1 (SPAC22A12.15c)


Gene Standard Namebip1 Characterisation Statuspublished
Systematic IDSPAC22A12.15c Feature Typeprotein coding
Synonymsbip Name Description
ProductER heat shock protein BiP Product Size663aa, 73.23 kDa
Genomic Location Chromosome I, 1185497-1182693 (2805nt); CDS:1185303-1183312 (1992nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding559
Annotation ExtensionEvidenceWith/FromReference
ATPase activity240
Annotation ExtensionEvidenceWith/FromReference
unfolded protein binding43
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cellular response to unfolded protein14
Annotation ExtensionEvidenceWith/FromReference
ER-associated ubiquitin-dependent protein catabolic process28
Annotation ExtensionEvidenceWith/FromReference
protein folding102
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum596
Annotation ExtensionEvidenceWith/FromReference
endoplasmic reticulum lumen13
Annotation ExtensionEvidenceWith/FromReference
Golgi apparatus357
Annotation ExtensionEvidenceWith/FromReference
nuclear membrane33
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationbip1Δ::ura4+1453
bip1Δ

Cell Phenotype

Term NameGenotypesCount
inviable sporebip1Δ476
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in alg6 glucosyltransferase Alg6
FYPO affected by mutation in cnx1 calnexin Cnx1
FYPO affected by mutation in gpt1 UDP-glucose-glycoprotein glucosyltransferase Gpt1
FYPO affected by mutation in ire1 serine/threonine protein kinase, sensor for unfolded proteins in the ER Ire1
FYPO affected by mutation in srp54 signal recognition particle subunit Srp54
FYPO localization affected by mutation in srp54 signal recognition particle subunit Srp54
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1185497..1182693
5' UTR1185497..1185304PMID:21511999
3' UTR1183311..1182693PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00012 Pfam IPR013126 Heat shock protein 70 family 37 641 8
TMhelix TMHMM 7 24 953
PS00297 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 40 47 5
PS00329 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 227 240 6
PS01036 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 364 378 7
PTHR19375 HMMPANTHER 33 658 8
3.90.640.10 Gene3D Cell shape determining protein MreB/Mrl 261 341 16
3.30.30.30 Gene3D Heat shock protein 70 family 86 146 6
3.30.420.40 Gene3D Carbohydrate kinase, FGGY 154 231 24
3.30.420.40 Gene3D Carbohydrate kinase, FGGY 37 85 24
1.20.1270.10 Gene3D IPR029048 Heat shock protein 70kD, C-terminal domain 546 648 6
2.60.34.10 Gene3D IPR029047 Heat shock protein 70kD, peptide-binding domain 419 545 7
3.30.420.40 Gene3D Carbohydrate kinase, FGGY 232 260 24
3.30.420.40 Gene3D Carbohydrate kinase, FGGY 346 387 24
SSF100920 SuperFamily IPR029047 Heat shock protein 70kD, peptide-binding domain 414 571 7
SSF100934 SuperFamily IPR029048 Heat shock protein 70kD, C-terminal domain 565 646 7
SSF53067 SuperFamily 35 218 25
SSF53067 SuperFamily 221 411 25
SignalP-noTM signalp 1 24 207
Coil ncoils Predicted coiled-coil protein (DUF2205) 290 310 1057
Coil ncoils Predicted coiled-coil protein (DUF2205) 543 563 1057
PR00301 PRINTS IPR013126 Heat shock protein 70 family 361 377 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 36 49 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 173 193 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 392 412 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 417 436 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 64 76 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 86 94 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 233 243 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 498 514 8

View domain organization at Pfam

Term IDTerm NameReferenceCount
SO:0000418signal_peptide279
SO:0001806ER_retention_signal3
residue A660-L663PMID:1373379

Protein Properties

Ave. residue weight 110.45 Da
Charge -28.00
Codon Adaptation Index 0.71
Isoelectric point 4.57
Molecular weight 73.23 kDa
Number of residues 663
Modifications

Protein Modifications

Term NameResidueCount
glycosylated residue 126
Annotation ExtensionEvidenceResidueReference
IDA PMID:1373379
NAS PMID:8227200
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0034605Northern assay evidencePMID:1373379

Quantitative Gene Expression

View graphical display of gene expression data for bip1 (SPAC22A12.15c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
515066during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
505443during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
515101during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
539781during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
507789during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
123452.29during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
35320.11during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
41during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
4.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4605
conserved in eukaryotes4515
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC22A12.15c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturespho4thiamine-repressible acid phosphatase Pho4 Affinity Capture-WesternPMID:9860839
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
affinity captured bycnx1calnexin Cnx1 Affinity Capture-WesternPMID:9860839
affinity captured bynak1PAK-related kinase Nak1 Affinity Capture-MSPMID:23462181
affinity captured bysog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
binds DNA-binding domain construct withget3GET complex ATPase subunit Get3 (predicted) Two-hybridPMID:26771498
External References
Database Identifier Description
NBRP SPAC22A12.15c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC22A12.15c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC22A12.15c BioGRID Interaction Datasets
Expression Viewer SPAC22A12.15c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC22A12.15c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC22A12.15c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC22A12.15c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC22A12.15c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC22A12.15c Transcriptome Viewer (Bähler Lab)
GEO SPAC22A12.15c GEO profiles
PInt SPAC22A12.15c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC22A12.15c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC22A12.15c Fission yeast phenotypic data & analysis
Cyclebase SPAC22A12.15c.1 Cell Cycle Data
SPD / RIKEN27/27C11Orfeome Localization Data
UniProtKB/SwissProtP3660478 kDa glucose-regulated protein homolog
ModBaseP36604Database of comparative protein structure models
STRINGP36604Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593245ER heat shock protein BiP
RefSeq mRNANM_001018642972h- ER heat shock protein BiP (bip1), mRNA

Literature for bip1

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016