bip1 (SPAC22A12.15c)


Gene Standard Namebip1 Characterisation Statuspublished
Systematic IDSPAC22A12.15c Feature Typeprotein coding
Synonymsbip Name Description
ProductER heat shock protein BiP Product Size663aa, 73.23 kDa
Genomic Location Chromosome I, 1185497-1182693 (2805nt); CDS:1185303-1183312 (1992nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037558
GO:0016887ATPase activityISOSGD:S000003571GO_REF:0000024232
GO:0005515protein bindingIPIcnx1PMID:9860839831
GO:0051082unfolded protein bindingISOSGD:S000003571GO_REF:000002442
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0051084'de novo' posttranslational protein foldingIDAPMID:98608392
GO:0030433ER-associated ubiquitin-dependent protein catabolic processISOSGD:S000003571GO_REF:000002427
GO:0034620cellular response to unfolded proteinISOSGD:S000003571GO_REF:000002415
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005794Golgi apparatusIDAPMID:16823372355
GO:0005783endoplasmic reticulumIDAPMID:16823372594
GO:0005788endoplasmic reticulum lumenIDAPMID:13733797
GO:0031965nuclear membraneIDAPMID:137337936
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopybip1ΔNullPECO:0000005, PECO:0000137PMID:236978061338
Microscopybip1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000049inviable cellCell growth assaybip1Δ::ura4+NullPMID:13733791449
FYPO:0002151inviable spore465
penetrance FYPO_EXT:0000001Microscopybip1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in alg6 glucosyltransferase Alg6 PMID:9813085
FYPO affected by mutation in cnx1 calnexin Cnx1 PMID:22682253
FYPO affected by mutation in gpt1 UDP-glucose-glycoprotein glucosyltransferase Gpt1 PMID:22682253
FYPO affected by mutation in ire1 serine/threonine protein kinase, sensor for unfolded proteins in the ER Ire1 PMID:22682253
FYPO affected by mutation in srp54 signal recognition particle subunit Srp54 PMID:7969124
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
111854971182693

UTRs

Region Coordinates Reference
five_prime_UTR1185497..1185304PMID:21511999
three_prime_UTR1183311..1182693PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00012 Pfam IPR013126 Heat shock protein 70 family 37 641 8
TMhelix TMHMM 7 24 959
PS01036 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 364 378 7
PS00329 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 227 240 6
PS00297 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 40 47 5
PTHR19375 HMMPANTHER 26 662 8
3.30.30.30 Gene3D 86 146 6
3.30.420.40 Gene3D 232 260 24
3.30.420.40 Gene3D 346 387 24
1.20.1270.10 Gene3D 546 648 6
3.30.420.40 Gene3D 37 85 24
3.30.420.40 Gene3D 154 231 24
3.90.640.10 Gene3D 261 341 16
2.60.34.10 Gene3D 419 545 7
SSF53067 SuperFamily 35 218 25
SSF100920 SuperFamily Heat shock protein 70kD, peptide-binding domain 414 571 7
SSF100934 SuperFamily Heat shock protein 70kD, C-terminal domain 565 646 7
SSF53067 SuperFamily 221 411 25
SignalP-noTM signalp 1 24 212
Coil ncoils Rabaptin coiled-coil domain 290 311 968
Coil ncoils Rabaptin coiled-coil domain 543 564 968
PR00301 PRINTS IPR013126 Heat shock protein 70 family 361 377 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 417 436 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 498 514 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 36 49 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 64 76 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 173 193 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 392 412 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 86 94 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 233 243 8

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0000418signal_peptide279
SO:0001806ER_retention_signalPMID:13733793

Protein Properties

Ave. residue weight 110.45 Da
Charge -28.00
Isoelectric point 4.57
Molecular weight 73.23 kDa
Number of residues 663
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00693glycosylated residueIDAPMID:137337926
NASPMID:8227200
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0034605Northern assay evidencePMID:1373379

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
515066during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
505443during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
515101during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
539781during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
507789during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
123452.29during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
35320.11during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
41during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
4.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
sog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
nak1PAK-related kinase Nak1 Affinity Capture-MSPMID:23462181
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
cnx1calnexin Cnx1 Affinity Capture-WesternPMID:9860839
Affinity Capture-WesternPMID:17230581
External References
Database Identifier Description
NBRP SPAC22A12.15c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC22A12.15c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC22A12.15c BioGRID Interaction Datasets
Expression Viewer SPAC22A12.15c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC22A12.15c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC22A12.15c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC22A12.15c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC22A12.15c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC22A12.15c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC22A12.15c Cell Cycle Data
GEO SPAC22A12.15c GEO profiles
PInt SPAC22A12.15c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC22A12.15c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC22A12.15c Fission yeast phenotypic data & analysis
SPD / RIKEN27/27C11Orfeome Localization Data
UniProtKB/SwissProtP3660478 kDa glucose-regulated protein homolog
ModBaseP36604Database of comparative protein structure models
STRINGP36604Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593245ER heat shock protein BiP
RefSeq mRNANM_001018642972h- ER heat shock protein BiP (bip1), mRNA
European Nucleotide ArchiveCAA45762.1ENA Protein Mapping
European Nucleotide ArchiveCAB16585.1ENA Protein Mapping
UniParcUPI000012BA5CUniProt Archive

Literature for bip1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014