rrn10 (SPAC22E12.08)


Gene Standard Namerrn10 Characterisation Statuspublished
Systematic IDSPAC22E12.08 Feature Typeprotein coding
Synonyms Name Description
ProductRNA polymerase I upstream activation factor complex subunit Rrn10 Product Size97aa, 11.01 kDa
Genomic Location Chromosome I, 5033373-5034586 (1214nt); CDS:5033478-5033823 (346nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
rDNA binding6
Annotation ExtensionEvidenceWith/FromReference
transcription factor activity, RNA polymerase I upstream control element sequence-specific binding3
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
positive regulation of transcription from RNA polymerase I promoter4
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
nucleolus366
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
RNA polymerase I upstream activating factor complex4
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationrrn10Δ1450

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic cell cyclerrn10Δ849
inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cellsrrn10Δ88
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons5033373..5033603, 5033656..5034586
Intron5033604..5033655
5' UTR5033373..5033477PMID:21511999
3' UTR5033824..5034586PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF05234 Pfam IPR022793 RNA polymerase I-specific transcription initiation factor Rrn10 2 97 1
PTHR28054:SF1 HMMPANTHER 1 97 1
PTHR28054 HMMPANTHER 1 97 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.54 Da
Charge -12.00
Codon Adaptation Index 0.41
Isoelectric point 3.90
Molecular weight 11.01 kDa
Number of residues 97
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for rrn10 (SPAC22E12.08)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.7during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.51during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3090
conserved in fungi only619
conserved in fungi4604
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Load gene that interact genetically with SPAC22E12.08 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withgim3prefoldin subunit 4, Gim3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withste20Rictor homolog, Ste20 Negative GeneticPMID:22681890
negative genetic interaction withies2Ino80 complex subunit Ies2 Negative GeneticPMID:22681890
negative genetic interaction withpsy2protein phosphatase PP4 complex subunit Psy2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmid1medial ring protein Mid1 Negative GeneticPMID:22681890
negative genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
negative genetic interaction withxap5xap-5-like protein Negative GeneticPMID:24957674
negative genetic interaction withcho1phosphatidyl-N-dimethylethanolamine N-methyltransferase Negative GeneticPMID:22681890
negative genetic interaction withufd2ubiquitin-protein ligase E4 Ufd2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withgim1prefoldin subunit 6, Gim1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
negative genetic interaction withspc34DASH complex subunit Spc34 Negative GeneticPMID:22681890
negative genetic interaction withreb1RNA polymerase I transcription termination factor/ RNA polymerase II transcription factor Reb1 Negative GeneticPMID:22681890
negative genetic interaction withvps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
positive genetic interaction withuap2U2 snRNP-associated protein Uap2 Positive GeneticPMID:22681890
positive genetic interaction withatg6autophagy associated beclin family protein Atg6 Positive GeneticPMID:22681890
positive genetic interaction withcdt2WD repeat protein Cdt2 Positive GeneticPMID:22681890
positive genetic interaction withpof9F-box protein Pof9 Positive GeneticPMID:22681890
positive genetic interaction withpst2Clr6 histone deacetylase complex subunit Pst2 Positive GeneticPMID:22681890
positive genetic interaction withsts5RNB-like protein Positive GeneticPMID:22681890
positive genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Positive GeneticPMID:21504829
positive genetic interaction withypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Positive GeneticPMID:22681890
positive genetic interaction withssp2AMP-activated protein serine/threonine kinase alpha subunit Ssp2 Positive GeneticPMID:22681890
positive genetic interaction withsrb11cyclin CycC, Srb mediator subunit Srb11 Positive GeneticPMID:22681890
positive genetic interaction withatp11mitochondrial F1-ATPase chaperone Atp11 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withplb1phospholipase B homolog Plb1 Positive GeneticPMID:22681890
positive genetic interaction withckb2CK2 family regulatory subunit Ckb2 (predicted) Positive GeneticPMID:22681890
positive genetic interaction witharp42SWI/SNF and RSC complex subunit Arp42 Positive GeneticPMID:22681890
positive genetic interaction withhrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Positive GeneticPMID:22681890
rescued bypst2Clr6 histone deacetylase complex subunit Pst2 Phenotypic SuppressionPMID:19547744
rescued bymga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Phenotypic SuppressionPMID:19547744
External References
Database Identifier Description
NBRP SPAC22E12.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC22E12.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC22E12.08 BioGRID Interaction Datasets
Expression Viewer SPAC22E12.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC22E12.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC22E12.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC22E12.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC22E12.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC22E12.08 Transcriptome Viewer (Bähler Lab)
GEO SPAC22E12.08 GEO profiles
PInt SPAC22E12.08 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC22E12.08 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC22E12.08 Fission yeast phenotypic data & analysis
Cyclebase SPAC22E12.08.1 Cell Cycle Data
SPD / RIKEN02/02B04Orfeome Localization Data
UniProtKB/SwissProtQ10360RNA polymerase I-specific transcription initiation factor rrn10
ModBaseQ10360Database of comparative protein structure models
STRINGQ10360Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594834RNA polymerase I upstream activation factor complex subunit Rrn10
RefSeq mRNANM_001020263972h- RNA polymerase I upstream activation factor complex subunit Rrn10 (rrn10), mRNA
European Nucleotide ArchiveCU329670ENA EMBL mapping
European Nucleotide ArchiveCAA93895ENA Protein Mapping
UniParcUPI000013A905UniProt Archive

Literature for rrn10

Search: Europe PMC or PubMed

Release Version: PomBase:30_57 - 27 Jan 2016