alp41 (SPAC22F3.05c)


Gene Standard Namealp41 Characterisation Statuspublished
Systematic IDSPAC22F3.05c Feature Typeprotein coding
Synonyms Name Description
ProductADP-ribosylation factor Alp41 Product Size186aa, 21.24 kDa
Genomic Location Chromosome I, 697191-703040 (5850nt); CDS:697291-698068 (778nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005525GTP bindingIEAUniProtKB-KW:KW-0342GO_REF:0000037113
GO:0003924GTPase activityISMIPR001806GO_REF:000002461
GO:0005515protein bindingIPItbc1PMID:23576550887
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006184GTP catabolic processISMIPR001806GO_REF:000002448
GO:0030952establishment or maintenance of cytoskeleton polarityIMPPMID:1068344619
GO:0006886intracellular protein transportNASGO_REF:0000051332
GO:0031110regulation of microtubule polymerization or depolymerizationIMPPMID:106834465
GO:0007264small GTPase mediated signal transductionIEAIPR024156GO_REF:000000258
GO:0007021tubulin complex assemblyISOSGD:S000004746GO_REF:000002420
GO:0072668tubulin complex biogenesisISOSGD:S000004746GO_REF:000002421
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005856cytoskeletonIEAUniProtKB-KW:KW-0206GO_REF:0000037390
GO:0005829cytosolIDAPMID:168233722317
GO:0005634nucleusIDAPMID:168233722730
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopyalp41ΔNullPECO:0000005, PECO:0000137PMID:236978061309
Microscopyalp41ΔNullPMID:20473289
FYPO:0000082sensitive to heatCell growth assayalp41-14 (E85K)Not specifiedPECO:0000137PMID:2357655082
Cell growth assayalp41-5 (L36Q)Not specifiedPECO:0000137PMID:23576550
Cell growth assayalp41-8 (R18W)Not specifiedPECO:0000137PMID:23576550
FYPO:0000091sensitive to thiabendazoleCell growth assayalp41-8 (R18W)Not specifiedPECO:0000137PMID:2357655092
Cell growth assayalp41-5 (L36Q)Not specifiedPECO:0000137PMID:23576550
Cell growth assayalp41-14 (E85K)Not specifiedPECO:0000137PMID:23576550

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000338abnormal mitotic spindleMicroscopyalp41-5 (L36Q)Not specifiedPECO:0000229, PECO:0000004PMID:2357655017
penetrance FYPO_EXT:0000002
FYPO:0000015branched vegetative cellMicroscopyalp41-5 (L36Q)Not specifiedPECO:0000229, PECO:0000004PMID:2357655020
penetrance FYPO_EXT:0000002
FYPO:0000016curved vegetative cellMicroscopyalp41-5 (L36Q)Not specifiedPECO:0000229, PECO:0000004PMID:2357655057
penetrance FYPO_EXT:0000002
FYPO:0002148inviable after spore germination, multiple cell divisions, abnormal morphologyMicroscopyalp41ΔNullPECO:0000005, PECO:0000137PMID:23697806177
penetrance FYPO_EXT:0000001
FYPO:0002004microtubules absent from cellMicroscopyalp41-5 (L36Q)Not specifiedPECO:0000229, PECO:0000004PMID:235765503
penetrance FYPO_EXT:0000002
FYPO:0002760short cytoplasmic microtubulesMicroscopyalp41-5 (L36Q)Not specifiedPECO:0000229, PECO:0000004PMID:235765504
penetrance FYPO_EXT:0000002
FYPO:0000645small vegetative cellMicroscopyalp41-5 (L36Q)Not specifiedPECO:0000229, PECO:0000004PMID:2357655051
penetrance FYPO_EXT:0000002
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1697191697355
2697401697455
3697496697568
4697621697766
5697805697885
6697928701453
7701508703040

UTRs

Region Start End Reference
three_prime_UTR701508703040PMID:21511999
three_prime_UTR698069701453PMID:21511999
five_prime_UTR697191697290PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00025 Pfam IPR006689 Small GTPase superfamily, ARF/SAR type 4 175 4
SM00177 SMART IPR024156 Small GTPase superfamily, ARF type 1 180 3
PS51417 Prosite Profiles IPR024156 Small GTPase superfamily, ARF type 10 176 3
PTHR11711 HMMPANTHER 1 178 7
PTHR11711:SF26 HMMPANTHER 1 178 1
G3DSA:3.40.50.300 Gene3D 3 176 279
SSF52540 SuperFamily IPR027417 13 180 294
PR00328 PRINTS IPR006689 Small GTPase superfamily, ARF/SAR type 18 41 4
PR00328 PRINTS IPR006689 Small GTPase superfamily, ARF/SAR type 118 139 4
PR00328 PRINTS IPR006689 Small GTPase superfamily, ARF/SAR type 46 70 4
PR00328 PRINTS IPR006689 Small GTPase superfamily, ARF/SAR type 73 98 4
TIGR00231 tigrfam IPR005225 Small GTP-binding protein domain 15 169 35

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.17 Da
Charge -2.50
Isoelectric point 5.11
Molecular weight 21.24 kDa
Number of residues 186
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
NDduring GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.96during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
1.7during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
tbc1tubulin specific chaperone cofactor C Tbc1 (predicted) Synthetic RescuePMID:23576550
alp1tubulin specific chaperone cofactor D, Alp1 Phenotypic SuppressionPMID:23576550
Synthetic Lethality
Dosage RescuePMID:10683446
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
tbc1tubulin specific chaperone cofactor C Tbc1 (predicted) Affinity Capture-WesternPMID:23576550
Protein-peptide
alp1tubulin specific chaperone cofactor D, Alp1 Protein-peptidePMID:23576550
Affinity Capture-Western
External References
Database Identifier Description
NBRP SPAC22F3.05c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC22F3.05c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC22F3.05c BioGRID Interaction Datasets
Expression Viewer SPAC22F3.05c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC22F3.05c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC22F3.05c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC22F3.05c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC22F3.05c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC22F3.05c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC22F3.05c Cell Cycle Data
GEO SPAC22F3.05c GEO profiles
PInt SPAC22F3.05c Protein-Protein Interaction Predictor (Bähler Lab)
SPD / RIKEN09/09F10Orfeome Localization Data
WikiGene2541769ADP-ribosylation factor Alp41
EntrezGene2541769ADP-ribosylation factor Alp41
UniProtKB/SwissProtQ09767ADP-ribosylation factor-like protein alp41
ModBaseQ09767Database of comparative protein structure models
StringQ09767Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593036ADP-ribosylation factor Alp41
RefSeq mRNANM_001018435972h- ADP-ribosylation factor Alp41 (alp41), mRNA
European Nucleotide ArchiveBAA83522ENA Protein Mapping
European Nucleotide ArchiveCAA91070ENA Protein Mapping
UniParcUPI0000125F18UniProt Archive

Literature for alp41

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014