alp41 (SPAC22F3.05c)


Gene Standard Namealp41 Characterisation Statuspublished
Systematic IDSPAC22F3.05c Feature Typeprotein coding
Synonyms Name Description
ProductGTP-binding protein involved in beta-tubulin folding Alp41 Product Size186aa, 21.24 kDa
Genomic Location Chromosome I, 697191-703040 (5850nt); CDS:697291-698068 (778nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
GTP binding113
Annotation ExtensionEvidenceWith/FromReference
GTPase activity62
Annotation ExtensionEvidenceWith/FromReference
protein binding872
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
tubulin complex assembly12
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
nucleus2691
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
decreased cell population growth at high temperaturealp41-14 (E85K)173
alp41-5 (L36Q)
alp41-8 (R18W)
inviable vegetative cell populationalp41Δ1452
sensitive to thiabendazolealp41-14 (E85K)327
alp41-8 (R18W)
alp41-5 (L36Q)

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic spindlealp41-5 (L36Q)62
curved vegetative cellalp41-5 (L36Q)120
inviable after spore germination, multiple cell divisions, abnormal morphologyalp41Δ205
inviable after spore germination, multiple cell divisions, branched, curved, elongated cellalp41Δ1
inviable branched, elongated vegetative cellalp41-5 (L36Q)23
microtubules absent from cellalp41-5 (L36Q)7
short cytoplasmic microtubulesalp41-5 (L36Q)20
small vegetative cellalp41-5 (L36Q)86

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased cell population growth at high temperaturetbc1-11 (F174S), alp41-8 (R18W)
normal vegetative cell population growthalp41-14 (E85K), tbc1-11 (F174S)
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons697191..697355, 697401..697455, 697496..697568, 697621..697766, 697805..697885, 697928..701453, 701508..703040
Intron697356..697400, 697456..697495, 697569..697620, 697767..697804, 697886..697927, 701454..701507
mRNA697191..703040
5' UTR697191..697290PMID:21511999
CDS697291..697355, 697401..697455, 697496..697568, 697621..697766, 697805..697885, 697928..698068
3' UTR698069..701453, 701508..703040PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00025 Pfam IPR006689 Small GTPase superfamily, ARF/SAR type 4 175 4
SM00177 SMART IPR024156 Small GTPase superfamily, ARF type 1 180 4
SM00175 SMART IPR003579 Small GTPase superfamily, Rab type 17 179 22
SM00178 SMART 3 176 4
PS51417 Prosite Profiles IPR024156 Small GTPase superfamily, ARF type 10 176 3
PTHR11711 HMMPANTHER 1 178 4
PTHR11711:SF26 HMMPANTHER 1 178 1
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 3 176 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 13 180 294
PR00328 PRINTS IPR006689 Small GTPase superfamily, ARF/SAR type 118 139 4
PR00328 PRINTS IPR006689 Small GTPase superfamily, ARF/SAR type 73 98 4
PR00328 PRINTS IPR006689 Small GTPase superfamily, ARF/SAR type 18 41 4
PR00328 PRINTS IPR006689 Small GTPase superfamily, ARF/SAR type 46 70 4
TIGR00231 TIGRFAM IPR005225 Small GTP-binding protein domain 15 169 35

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.17 Da
Charge -2.50
Codon Adaptation Index 0.34
Isoelectric point 5.11
Molecular weight 21.24 kDa
Number of residues 186
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for alp41 (SPAC22F3.05c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2403during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.7during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.96during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC22F3.05c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturestbc1tubulin specific chaperone cofactor C, GTPase activating protein Tbc1 (predicted) Affinity Capture-WesternPMID:23576550
affinity capturesalp1tubulin specific chaperone cofactor D, Alp1 Affinity Capture-WesternPMID:23576550
(peptide) binds to proteintbc1tubulin specific chaperone cofactor C, GTPase activating protein Tbc1 (predicted) Protein-peptidePMID:23576550
binds to peptidetbc1tubulin specific chaperone cofactor C, GTPase activating protein Tbc1 (predicted) Protein-peptidePMID:23576550
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC22F3.05c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
rescued by overexpression ofalp1tubulin specific chaperone cofactor D, Alp1 Dosage RescuePMID:10683446
rescuesalp1tubulin specific chaperone cofactor D, Alp1 Phenotypic SuppressionPMID:23576550
synthetically rescuestbc1tubulin specific chaperone cofactor C, GTPase activating protein Tbc1 (predicted) Synthetic RescuePMID:23576550
synthetic lethal withalp1tubulin specific chaperone cofactor D, Alp1 Synthetic LethalityPMID:23576550
External References
Database Identifier Description
NBRP SPAC22F3.05c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC22F3.05c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC22F3.05c BioGRID Interaction Datasets
Expression Viewer SPAC22F3.05c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC22F3.05c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC22F3.05c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC22F3.05c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC22F3.05c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC22F3.05c Transcriptome Viewer (Bähler Lab)
GEO SPAC22F3.05c GEO profiles
PInt SPAC22F3.05c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC22F3.05c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC22F3.05c Fission yeast phenotypic data & analysis
Cyclebase SPAC22F3.05c.1 Cell Cycle Data
SPD / RIKEN09/09F10Orfeome Localization Data
UniProtKB/SwissProtQ09767ADP-ribosylation factor-like protein alp41
ModBaseQ09767Database of comparative protein structure models
STRINGQ09767Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593036ADP-ribosylation factor Alp41
European Nucleotide ArchiveBAA83522.1ENA Protein Mapping
European Nucleotide ArchiveCAA91070.1ENA Protein Mapping
UniParcUPI0000125F18UniProt Archive

Literature for alp41

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016