alp41 (SPAC22F3.05c)


Gene Standard Namealp41 Characterisation Statuspublished
Systematic IDSPAC22F3.05c Feature Typeprotein coding
Synonyms Name Description
ProductADP-ribosylation factor Alp41 Product Size186aa, 21.24 kDa
Genomic Location Chromosome I, 697191-703040 (5850nt); CDS:697291-698068 (778nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
GTP binding113
Annotation ExtensionEvidenceWith/FromReference
GTPase activity62
Annotation ExtensionEvidenceWith/FromReference
protein binding848
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
establishment or maintenance of cytoskeleton polarity18
Annotation ExtensionEvidenceWith/FromReference
intracellular protein transport340
Annotation ExtensionEvidenceWith/FromReference
regulation of microtubule polymerization or depolymerization9
Annotation ExtensionEvidenceWith/FromReference
small GTPase mediated signal transduction139
Annotation ExtensionEvidenceWith/FromReference
tubulin complex assembly20
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoskeleton394
Annotation ExtensionEvidenceWith/FromReference
cytosol2316
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growth at high temperaturealp41-14 (E85K)Not specified151
alp41-5 (L36Q)Not specified
alp41-8 (R18W)Not specified
inviable vegetative cell populationalp41ΔNull1427
sensitive to thiabendazolealp41-14 (E85K)Not specified185
alp41-5 (L36Q)Not specified
alp41-8 (R18W)Not specified

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic spindle40
penetrance FYPO_EXT:0000002alp41-5 (L36Q)Not specified
branched vegetative cell57
penetrance FYPO_EXT:0000002alp41-5 (L36Q)Not specified
curved vegetative cell103
penetrance FYPO_EXT:0000002alp41-5 (L36Q)Not specified
inviable after spore germination, multiple cell divisions, abnormal morphology192
penetrance FYPO_EXT:0000001alp41ΔNull
microtubules absent from cell3
penetrance FYPO_EXT:0000002alp41-5 (L36Q)Not specified
short cytoplasmic microtubules13
penetrance FYPO_EXT:0000002alp41-5 (L36Q)Not specified
small vegetative cell67
penetrance FYPO_EXT:0000002alp41-5 (L36Q)Not specified
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1697191697355
2697401697455
3697496697568
4697621697766
5697805697885
6697928701453
7701508703040

UTRs

Region Coordinates Reference
five_prime_UTR697191..697290PMID:21511999
three_prime_UTR698069..701453,701508..703040PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00025 Pfam IPR006689 Small GTPase superfamily, ARF/SAR type 4 175 4
SM00177 SMART IPR024156 Small GTPase superfamily, ARF type 1 180 4
SM00175 SMART IPR003579 Small GTPase superfamily, Rab type 17 179 22
SM00178 SMART IPR006687 Small GTPase superfamily, SAR1-type 3 176 4
PS51417 Prosite Profiles IPR024156 Small GTPase superfamily, ARF type 10 176 3
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 3 176 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 13 180 294
PR00328 PRINTS IPR006689 Small GTPase superfamily, ARF/SAR type 118 139 4
PR00328 PRINTS IPR006689 Small GTPase superfamily, ARF/SAR type 73 98 4
PR00328 PRINTS IPR006689 Small GTPase superfamily, ARF/SAR type 46 70 4
PR00328 PRINTS IPR006689 Small GTPase superfamily, ARF/SAR type 18 41 4
TIGR00231 tigrfam IPR005225 Small GTP-binding protein domain 15 169 35

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.17 Da
Charge -2.50
Isoelectric point 5.11
Molecular weight 21.24 kDa
Number of residues 186
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2403during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.7during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.96during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
tbc1tubulin specific chaperone cofactor C, GTPase activating protein Tbc1 (predicted) Protein-peptidePMID:23576550
Affinity Capture-Western
alp1tubulin specific chaperone cofactor D, Alp1 Affinity Capture-WesternPMID:23576550
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
tbc1tubulin specific chaperone cofactor C, GTPase activating protein Tbc1 (predicted) Synthetic RescuePMID:23576550
alp1tubulin specific chaperone cofactor D, Alp1 Synthetic LethalityPMID:23576550
Phenotypic Suppression
Dosage RescuePMID:10683446
External References
Database Identifier Description
NBRP SPAC22F3.05c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC22F3.05c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC22F3.05c BioGRID Interaction Datasets
Expression Viewer SPAC22F3.05c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC22F3.05c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC22F3.05c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC22F3.05c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC22F3.05c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC22F3.05c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC22F3.05c Cell Cycle Data
GEO SPAC22F3.05c GEO profiles
PInt SPAC22F3.05c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC22F3.05c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC22F3.05c Fission yeast phenotypic data & analysis
SPD / RIKEN09/09F10Orfeome Localization Data
UniProtKB/SwissProtQ09767ADP-ribosylation factor-like protein alp41
ModBaseQ09767Database of comparative protein structure models
STRINGQ09767Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593036ADP-ribosylation factor Alp41
European Nucleotide ArchiveBAA83522.1ENA Protein Mapping
European Nucleotide ArchiveCAA91070.1ENA Protein Mapping
UniParcUPI0000125F18UniProt Archive

Literature for alp41

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015