alp41 (SPAC22F3.05c)


Gene Standard Namealp41 Characterisation Statuspublished
Systematic IDSPAC22F3.05c Feature Typeprotein coding
Synonyms Name Description
ProductADP-ribosylation factor Alp41 Product Size186aa, 21.24 kDa
Genomic Location Chromosome I, 697191-703040 (5850nt); CDS:697291-698068 (778nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005525GTP bindingIEAUniProtKB-KW:KW-0342GO_REF:0000037113
GO:0003924GTPase activityISMIPR001806GO_REF:000002461
GO:0005515protein bindingIPItbc1PMID:23576550865
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006184GTP catabolic processISMIPR001806GO_REF:000002448
GO:0030952establishment or maintenance of cytoskeleton polarityIMPPMID:1068344619
GO:0006886intracellular protein transportNASGO_REF:0000051326
GO:0031110regulation of microtubule polymerization or depolymerizationIMPPMID:106834468
GO:0007264small GTPase mediated signal transductionIEAIPR024156GO_REF:000000263
GO:0007021tubulin complex assemblyISOSGD:S000004746GO_REF:000002420
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005856cytoskeletonIEAUniProtKB-KW:KW-0206GO_REF:0000037393
GO:0005829cytosolIDAPMID:168233722319
GO:0005634nucleusIDAPMID:168233722737
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000082decreased cell population growth at high temperatureCell growth assayalp41-14 (E85K)Not specifiedPECO:0000137PMID:23576550144
Cell growth assayalp41-5 (L36Q)Not specifiedPECO:0000137PMID:23576550
Cell growth assayalp41-8 (R18W)Not specifiedPECO:0000137PMID:23576550
FYPO:0002061inviable vegetative cell populationMicroscopyalp41ΔNullPMID:204732891337
Microscopyalp41ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0000091sensitive to thiabendazoleCell growth assayalp41-14 (E85K)Not specifiedPECO:0000137PMID:23576550175
Cell growth assayalp41-8 (R18W)Not specifiedPECO:0000137PMID:23576550
Cell growth assayalp41-5 (L36Q)Not specifiedPECO:0000137PMID:23576550

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000338abnormal mitotic spindle24
penetrance FYPO_EXT:0000002Microscopyalp41-5 (L36Q)Not specifiedPECO:0000229, PECO:0000004PMID:23576550
FYPO:0000015branched vegetative cell54
penetrance FYPO_EXT:0000002Microscopyalp41-5 (L36Q)Not specifiedPECO:0000229, PECO:0000004PMID:23576550
FYPO:0000016curved vegetative cell101
penetrance FYPO_EXT:0000002Microscopyalp41-5 (L36Q)Not specifiedPECO:0000229, PECO:0000004PMID:23576550
FYPO:0002148inviable after spore germination, multiple cell divisions, abnormal morphology187
penetrance FYPO_EXT:0000001Microscopyalp41ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0002004microtubules absent from cell3
penetrance FYPO_EXT:0000002Microscopyalp41-5 (L36Q)Not specifiedPECO:0000229, PECO:0000004PMID:23576550
FYPO:0002760short cytoplasmic microtubules6
penetrance FYPO_EXT:0000002Microscopyalp41-5 (L36Q)Not specifiedPECO:0000229, PECO:0000004PMID:23576550
FYPO:0000645small vegetative cell56
penetrance FYPO_EXT:0000002Microscopyalp41-5 (L36Q)Not specifiedPECO:0000229, PECO:0000004PMID:23576550
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1697191697355
2697401697455
3697496697568
4697621697766
5697805697885
6697928701453
7701508703040
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00025 Pfam IPR006689 Small GTPase superfamily, ARF/SAR type 4 175 4
SM00178 SMART IPR006687 Small GTPase superfamily, SAR1-type 3 176 4
SM00177 SMART IPR024156 Small GTPase superfamily, ARF type 1 180 4
SM00175 SMART IPR003579 Small GTPase superfamily, Rab type 17 179 22
PS51417 Prosite Profiles IPR024156 Small GTPase superfamily, ARF type 10 176 3
PTHR11711 HMMPANTHER 1 178 7
PTHR11711:SF26 HMMPANTHER 1 178 1
3.40.50.300 Gene3D 3 176 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 13 180 294
PR00328 PRINTS IPR006689 Small GTPase superfamily, ARF/SAR type 118 139 4
PR00328 PRINTS IPR006689 Small GTPase superfamily, ARF/SAR type 46 70 4
PR00328 PRINTS IPR006689 Small GTPase superfamily, ARF/SAR type 73 98 4
PR00328 PRINTS IPR006689 Small GTPase superfamily, ARF/SAR type 18 41 4
TIGR00231 tigrfam IPR005225 Small GTP-binding protein domain 15 169 35

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.17 Da
Charge -2.50
Isoelectric point 5.11
Molecular weight 21.24 kDa
Number of residues 186
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
NDduring GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
2403during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.7during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.96during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
tbc1tubulin specific chaperone cofactor C, GTPase activating protein Tbc1 (predicted) Synthetic RescuePMID:23576550
alp1tubulin specific chaperone cofactor D, Alp1 Synthetic LethalityPMID:23576550
Phenotypic Suppression
Dosage RescuePMID:10683446
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
tbc1tubulin specific chaperone cofactor C, GTPase activating protein Tbc1 (predicted) Affinity Capture-WesternPMID:23576550
Protein-peptide
alp1tubulin specific chaperone cofactor D, Alp1 Affinity Capture-WesternPMID:23576550
External References
Database Identifier Description
NBRP SPAC22F3.05c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC22F3.05c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC22F3.05c BioGRID Interaction Datasets
Expression Viewer SPAC22F3.05c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC22F3.05c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC22F3.05c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC22F3.05c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC22F3.05c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC22F3.05c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC22F3.05c Cell Cycle Data
GEO SPAC22F3.05c GEO profiles
PInt SPAC22F3.05c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC22F3.05c Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN09/09F10Orfeome Localization Data
UniProtKB/SwissProtQ09767ADP-ribosylation factor-like protein alp41
ModBaseQ09767Database of comparative protein structure models
STRINGQ09767Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593036ADP-ribosylation factor Alp41
European Nucleotide ArchiveBAA83522.1ENA Protein Mapping
European Nucleotide ArchiveCAA91070.1ENA Protein Mapping
UniParcUPI0000125F18UniProt Archive

Literature for alp41

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014