rok1 (SPAC22F3.08c)


Gene Standard Namerok1 Characterisation Statusbiological_role_inferred
Systematic IDSPAC22F3.08c Feature Typeprotein coding
Synonyms Name Description
ProductATP-dependent RNA helicase Rok1 (predicted) Product Size481aa, 54.03 kDa
Genomic Location Chromosome I, 689345-691122 (1778nt); CDS:689483-690928 (1446nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
ATP-dependent RNA helicase activity40
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

ribosome biogenesis


Term NameCount
rRNA processing197
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
nucleolus359
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
slow vegetative cell population growthrok1ΔNull326
viable vegetative cell populationrok1ΔNull3795

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologyrok1ΔNull3093
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons689345..691122
mRNA689345..691122
5' UTR689345..689482PMID:21511999
CDS689483..690928
3' UTR690929..691122PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00270 Pfam IPR011545 DEAD/DEAH box helicase domain 70 238 43
PF00271 Pfam IPR001650 Helicase, C-terminal 311 383 68
SM00490 SMART IPR001650 Helicase, C-terminal 299 383 68
SM00487 SMART IPR014001 Helicase superfamily 1/2, ATP-binding domain 65 264 69
PS51194 Prosite Profiles IPR001650 Helicase, C-terminal 260 422 69
PS51192 Prosite Profiles IPR014001 Helicase superfamily 1/2, ATP-binding domain 77 249 69
PS00039 Prosite Patterns IPR000629 ATP-dependent RNA helicase DEAD-box, conserved site 194 202 19
PTHR24031 HMMPANTHER 23 462 25
PTHR24031:SF33 HMMPANTHER 23 462 1
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 255 415 288
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 48 254 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 78 395 294
Coil ncoils Rabaptin coiled-coil domain 146 167 968

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000174DEAD/DEAH box helicaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000174

Protein Properties

Ave. residue weight 112.32 Da
Charge 27.00
Isoelectric point 10.11
Molecular weight 54.03 kDa
Number of residues 481
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1596during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1820during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1813during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1703during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1857during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3128.05during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
282.51during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.3during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.33during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancercancer_census142
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withabc2glutathione S-conjugate-exporting ATPase Abc2 Negative GeneticPMID:22681890
negative genetic interaction withace2transcription factor Ace2 Negative GeneticPMID:22681890
negative genetic interaction withacp2F-actin capping protein beta subunit Acp2 Negative GeneticPMID:22681890
negative genetic interaction withago1argonaute Negative GeneticPMID:22681890
negative genetic interaction withams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
negative genetic interaction witharp6actin-like protein Arp6 Negative GeneticPMID:18818364
positive genetic interaction withasc1cofactor for cytoplasmic methionyl-and glutamyl-tRNA synthetases Asc1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:18818364
negative genetic interaction withatp11mitochondrial F1-ATPase chaperone Atp11 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withatp11mitochondrial F1-ATPase chaperone Atp11 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withatp12mitochondrial F1-ATPase chaperone Atp12 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withatp14F1-ATPase subunit H (predicted) Negative GeneticPMID:22681890
negative genetic interaction withbdc1bromodomain containing protein 1, Bdc1 Negative GeneticPMID:18818364
negative genetic interaction withbdc1bromodomain containing protein 1, Bdc1 Negative GeneticPMID:22681890
negative genetic interaction withbdf2BET family double bromodomain protein Bdf2 Negative GeneticPMID:18818364
negative genetic interaction withbob1prefoldin subunit 5 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withbob1prefoldin subunit 5 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withbun62WD repeat protein, human WDR20 family, Bun62 Negative GeneticPMID:22681890
positive genetic interaction withcay1cactin, spliceosome complex subunit (predicted) Positive GeneticPMID:18818364
negative genetic interaction withcbp1CENP-B homolog Negative GeneticPMID:18818364
positive genetic interaction withcch1calcium channel Cch1 Positive GeneticPMID:22681890
negative genetic interaction withcdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
negative genetic interaction withcho1phosphatidyl-N-methylethanolamine N-methyltransferase (predicted) Negative GeneticPMID:18818364
negative genetic interaction withclr1SHREC complex subunit Clr1 Negative GeneticPMID:22681890
negative genetic interaction withclr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:18818364
negative genetic interaction withclr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:22681890
negative genetic interaction withcmk2MAPK-activated protein kinase Cmk2 Negative GeneticPMID:22681890
negative genetic interaction withcoq10mitochondrial ubiquinone binding protein Coq10 Negative GeneticPMID:22681890
negative genetic interaction withdcr1dicer Negative GeneticPMID:22681890
negative genetic interaction withddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:18818364
positive genetic interaction withdot2ESCRT II complex subunit Dot2 Positive GeneticPMID:18818364
negative genetic interaction withdph1UBA domain protein Dph1 Negative GeneticPMID:18818364
negative genetic interaction withdph2diphthamide biosynthesis protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withdph5diphthine synthase Dph5 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withdsc5UBX domain containing protein required for Sre1 cleavage Negative GeneticPMID:22681890
negative genetic interaction withdsk1SR protein-specific kinase Dsk1 Negative GeneticPMID:18818364
negative genetic interaction withdsk1SR protein-specific kinase Dsk1 Negative GeneticPMID:22681890
negative genetic interaction withefc25Ras1 guanyl-nucleotide exchange factor Efc25 Negative GeneticPMID:22681890
positive genetic interaction withelp3elongator complex subunit Elp3 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withelp4elongator complex subunit Elp4 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withelp6elongator complex subunit Elp6 (predicted) Positive GeneticPMID:18818364
negative genetic interaction witheme1Holliday junction resolvase subunit Eme1 Negative GeneticPMID:18818364
positive genetic interaction witheme1Holliday junction resolvase subunit Eme1 Positive GeneticPMID:22681890
negative genetic interaction withers1RNA-silencing factor Ers1 Negative GeneticPMID:22681890
negative genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:18818364
negative genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
positive genetic interaction withfkbp39FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) Positive GeneticPMID:18818364
positive genetic interaction withfkbp39FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withgcd10tRNA (m1A) methyltransferase Gcd10 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withgim1prefoldin subunit 6, Gim1 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withgrx5monothiol glutaredoxin Grx5 Negative GeneticPMID:22681890
negative genetic interaction withgyp1GTPase activating protein Gyp1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhhf1histone H4 h4.1 Negative GeneticPMID:22681890
negative genetic interaction withhip1hira protein, histone chaperone Hip1 Negative GeneticPMID:18818364
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:18818364
negative genetic interaction withhrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:22681890
negative genetic interaction withidp1isocitrate dehydrogenase Idp1 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withiwr1RNA polymerase II nuclear import protein Iwr1 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withiwr1RNA polymerase II nuclear import protein Iwr1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withlys2homoaconitate hydratase Lys2 Negative GeneticPMID:22681890
positive genetic interaction withmga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Positive GeneticPMID:18818364
positive genetic interaction withmoe1translation initiation factor eIF3d Moe1 Positive GeneticPMID:22681890
negative genetic interaction withmph1dual specificity protein kinase Mph1 Negative GeneticPMID:22681890
positive genetic interaction withmrpl1mitochondrial ribosomal protein subunit L1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withmug154conserved fungal protein Negative GeneticPMID:22681890
negative genetic interaction withmug178mitochondrial ribosomal protein subunit L51-b (predicted) Negative GeneticPMID:22681890
negative genetic interaction withnem1Nem1-Spo7 phosphatase complex catalytic subunit Nem1 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withnot2CCR4-Not complex subunit Not2 (predicted) Positive GeneticPMID:18818364
negative genetic interaction withnrm1MBF complex corepressor Nrm1 Negative GeneticPMID:22681890
negative genetic interaction withomh6alpha-1,2-mannosyltransferase Omh6 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpds5mitotic cohesin-associated protein Pds5 Negative GeneticPMID:18818364
negative genetic interaction withpek1MAP kinase kinase Pek1 Negative GeneticPMID:22681890
positive genetic interaction withpex11peroxisomal biogenesis factor 11 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withpht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:18818364
negative genetic interaction withpmk1MAP kinase Pmk1 Negative GeneticPMID:18818364
negative genetic interaction withpnk1DNA kinase/phosphatase Pnk1 Negative GeneticPMID:18818364
negative genetic interaction withppk21serine/threonine protein kinase Ppk21 Negative GeneticPMID:22681890
negative genetic interaction withppk26protein kinase like PAN complex subunit Ppk26 Negative GeneticPMID:22681890
positive genetic interaction withppr2mitochondrial PPR repeat protein Ppr2 Positive GeneticPMID:22681890
negative genetic interaction withppr5mitochondrial PPR repeat protein Ppr5 Negative GeneticPMID:22681890
negative genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
positive genetic interaction withpsh3ER chaperone SHR3 homologue Psh3 Positive GeneticPMID:22681890
negative genetic interaction withptp4phosphatidate cytidylyltransferase Ptp4 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withpuf6Puf family RNA-binding protein Puf6 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withrad2514-3-3 protein Rad25 Negative GeneticPMID:18818364
negative genetic interaction withrad8ubiquitin-protein ligase E3 Rad8 Negative GeneticPMID:18818364
negative genetic interaction withraf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Negative GeneticPMID:22681890
negative genetic interaction withraf2Rik1-associated factor Raf2 Negative GeneticPMID:18818364
negative genetic interaction withraf2Rik1-associated factor Raf2 Negative GeneticPMID:22681890
negative genetic interaction withrdp1RNA-directed RNA polymerase Rdp1 Negative GeneticPMID:18818364
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
negative genetic interaction withrpa12DNA-directed RNA polymerase complex I subunit Rpa12 Negative GeneticPMID:18818364
positive genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withrpl190260S ribosomal protein L19 Positive GeneticPMID:22681890
positive genetic interaction withrpl210260S ribosomal protein L21 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withrpl230160S ribosomal protein L23 Positive GeneticPMID:22681890
positive genetic interaction withrpl370260S ribosomal protein L37 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withrpl380160S ribosomal protein L38 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withrpl70260S ribosomal protein L7 Positive GeneticPMID:22681890
positive genetic interaction withrpl90160S ribosomal protein L9 Positive GeneticPMID:22681890
positive genetic interaction withrps00140S ribosomal protein S0A (p40) Positive GeneticPMID:22681890
positive genetic interaction withrps120140S ribosomal protein S12 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withrps160240S ribosomal protein S16 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withrps170240S ribosomal protein S17 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withrps2340S ribosomal protein S23 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withrps280240S ribosomal protein S28, Rps2802 Positive GeneticPMID:22681890
positive genetic interaction withrps40140S ribosomal protein S4 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withrsc1RSC complex subunit Rsc1 Negative GeneticPMID:18818364
negative genetic interaction withrsv2transcription factor Rsv2 Negative GeneticPMID:18818364
negative genetic interaction withscs7sphingosine hydroxylase Scs7 Negative GeneticPMID:18818364
negative genetic interaction withsec28coatomer epsilon subunit (predicted) Negative GeneticPMID:18818364
negative genetic interaction withset1histone lysine methyltransferase Set1 Negative GeneticPMID:18818364
negative genetic interaction withset3histone lysine methyltransferase Set3 Negative GeneticPMID:18818364
negative genetic interaction withset3histone lysine methyltransferase Set3 Negative GeneticPMID:22681890
negative genetic interaction withsgf73SAGA complex subunit Sgf73 Negative GeneticPMID:25076038
positive genetic interaction withsnr13-hydroxyisobutyryl-CoA hydrolase snr1 Positive GeneticPMID:22681890
positive genetic interaction withSPAC14C4.11polyphosphate synthetase (predicted) Positive GeneticPMID:22681890
negative genetic interaction withSPAC22H10.09Schizosaccharomyces specific protein Negative GeneticPMID:22681890
negative genetic interaction withSPAC26A3.11amidohydrolase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC824.09cGTPase activating protein (predicted) Negative GeneticPMID:22681890
positive genetic interaction withSPAPB1E7.11cSchizosaccharomyces specific protein Positive GeneticPMID:22681890
negative genetic interaction withSPBC16A3.10membrane bound O-acyltransferase, MBOAT (predicted) Negative GeneticPMID:22681890
positive genetic interaction withSPBC17A3.06phosphoprotein phosphatase (predicted) Positive GeneticPMID:22681890
negative genetic interaction withSPBC24C6.04delta-1-pyrroline-5-carboxylate dehydrogenase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC365.16conserved protein Negative GeneticPMID:22681890
negative genetic interaction withSPBC725.01aspartate aminotransferase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC8E4.05cfumarate lyase superfamily Negative GeneticPMID:22681890
negative genetic interaction withSPCC1393.08transcription factor, zf-GATA type (predicted) Negative GeneticPMID:22681890
positive genetic interaction withSPCC550.03cSki complex RNA helicase Ski2 (predicted) Positive GeneticPMID:18818364
negative genetic interaction withspf1Set1C PHD Finger protein Spf1 Negative GeneticPMID:18818364
negative genetic interaction withsrb11cyclin CycC, Srb mediator subunit Srb11 Negative GeneticPMID:18818364
negative genetic interaction withsrs2ATP-dependent DNA helicase, UvrD subfamily Negative GeneticPMID:18818364
negative genetic interaction withsum2G2/M transition checkpoint protein Sum2 Negative GeneticPMID:22681890
negative genetic interaction withswd3WD repeat protein Swd3 Negative GeneticPMID:18818364
negative genetic interaction withswi2Swi5 complex subunit Swi2 Negative GeneticPMID:18818364
negative genetic interaction withtas3RITS complex subunit 3 Negative GeneticPMID:22681890
positive genetic interaction withtom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withtrt1telomerase reverse transcriptase 1 protein Trt1 Negative GeneticPMID:22681890
negative genetic interaction withubc6ubiquitin conjugating enzyme E2 Ubc6 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withutp16U3 snoRNP-associated protein Utp16 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withvht1vitamin H transmembrane transporter Vht1 Negative GeneticPMID:22681890
positive genetic interaction withvps38phophatidylinositol 3-kinase complex subunit Vps38 Positive GeneticPMID:22681890
negative genetic interaction withvps71Swr1 complex subunit Vps71 Negative GeneticPMID:18818364
negative genetic interaction withyaf9YEATS family histone acetyltransferase subunit Yaf9 Negative GeneticPMID:18818364
positive genetic interaction withyar1ribosome biogenesis protein Yar1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withzds1zds family protein phosphatase type A regulator Zds1 (predicted) Negative GeneticPMID:18818364
External References
Database Identifier Description
NBRP SPAC22F3.08c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC22F3.08c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC22F3.08c BioGRID Interaction Datasets
Expression Viewer SPAC22F3.08c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC22F3.08c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC22F3.08c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC22F3.08c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC22F3.08c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC22F3.08c Transcriptome Viewer (Bähler Lab)
GEO SPAC22F3.08c GEO profiles
PInt SPAC22F3.08c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC22F3.08c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC22F3.08c Fission yeast phenotypic data & analysis
Cyclebase SPAC22F3.08c.1 Cell Cycle Data
SPD / RIKEN23/23C07Orfeome Localization Data
UniProtKB/SwissProtQ09775ATP-dependent RNA helicase rok1
ModBaseQ09775Database of comparative protein structure models
STRINGQ09775Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593033ATP-dependent RNA helicase Rok1 (predicted)
RefSeq mRNANM_001018432972h- ATP-dependent RNA helicase Rok1 (predicted) (rok1), mRNA
European Nucleotide ArchiveCAA91073.1ENA Protein Mapping
UniParcUPI0000139FCAUniProt Archive

Literature for rok1

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015