rok1 (SPAC22F3.08c)


Gene Standard Namerok1 Characterisation Statusbiological_role_inferred
Systematic IDSPAC22F3.08c Feature Typeprotein coding
Synonyms Name Description
ProductATP-dependent RNA helicase Rok1 (predicted) Product Size481aa, 54.03 kDa
Genomic Location Chromosome I, 689345-691122 (1778nt); CDS:689483-690928 (1446nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037559
GO:0004004ATP-dependent RNA helicase activityISOSGD:S000003139GO_REF:000002440
GO:0003723RNA bindingIEAUniProtKB-KW:KW-0694GO_REF:0000037628
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006364rRNA processingISOSGD:S000003139GO_REF:0000024181
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722316
GO:0005730nucleolusISOSGD:S000003139GO_REF:0000024355
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopyrok1ΔNullPMID:204732893760
Microscopyrok1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3086
penetrance FYPO_EXT:0000001Microscopyrok1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1689345691122

UTRs

Region Coordinates Reference
five_prime_UTR689345..689482PMID:21511999
three_prime_UTR690929..691122PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00270 Pfam IPR011545 DNA/RNA helicase, DEAD/DEAH box type, N-terminal 70 238 43
PF00271 Pfam IPR001650 Helicase, C-terminal 311 383 68
SM00490 SMART IPR001650 Helicase, C-terminal 299 383 67
SM00487 SMART IPR014001 Helicase, superfamily 1/2, ATP-binding domain 65 264 70
PS00039 Prosite Patterns IPR000629 RNA helicase, ATP-dependent, DEAD-box, conserved site 194 202 19
PS51192 Prosite Profiles IPR014001 Helicase, superfamily 1/2, ATP-binding domain 77 249 69
PS51194 Prosite Profiles IPR001650 Helicase, C-terminal 260 422 69
PTHR24031:SF33 HMMPANTHER 23 462 1
PTHR24031 HMMPANTHER 23 462 25
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 48 254 279
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 255 415 279
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 78 395 294
Coil ncoils Rabaptin coiled-coil domain 146 167 968

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000174DEAD/DEAH box helicaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000174

Protein Properties

Ave. residue weight 112.32 Da
Charge 27.00
Isoelectric point 10.11
Molecular weight 54.03 kDa
Number of residues 481
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
282.51during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
3128.05during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
1857during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
1820during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
1596during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
1703during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
1813during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.33during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
1.3during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancercancer_census141
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
pnk1DNA kinase/phosphatase Pnk1 Negative GeneticPMID:18818364
yaf9YEATS family histone acetyltransferase subunit Yaf9 Negative GeneticPMID:18818364
SPAC824.09cGTPase activating protein (predicted) Negative GeneticPMID:22681890
hip1hira protein, histone chaperone Hip1 Negative GeneticPMID:18818364
mph1dual specificity protein kinase Mph1 Negative GeneticPMID:22681890
bdc1bromodomain containing protein 1, Bdc1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
mga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Positive GeneticPMID:18818364
ers1RNA-silencing factor Ers1 Negative GeneticPMID:22681890
SPCC550.03cSki complex RNA helicase Ski2 (predicted) Positive GeneticPMID:18818364
SPBC24C6.04delta-1-pyrroline-5-carboxylate dehydrogenase (predicted) Negative GeneticPMID:22681890
elp3elongator complex subunit Elp3 (predicted) Positive GeneticPMID:22681890
atp14F1-ATPase subunit H (predicted) Negative GeneticPMID:22681890
ddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:18818364
dph2diphthamide biosynthesis protein (predicted) Negative GeneticPMID:22681890
gyp1GTPase activating protein Gyp1 (predicted) Negative GeneticPMID:22681890
rpl370260S ribosomal protein L37 (predicted) Positive GeneticPMID:22681890
ppk21serine/threonine protein kinase Ppk21 Negative GeneticPMID:22681890
SPCC1393.08transcription factor, zf-GATA type (predicted) Negative GeneticPMID:22681890
SPBC8E4.05cfumarate lyase superfamily Negative GeneticPMID:22681890
SPAC14C4.11polyphosphate synthetase (predicted) Positive GeneticPMID:22681890
tas3RITS complex subunit 3 Negative GeneticPMID:22681890
dph5diphthine synthase Dph5 (predicted) Negative GeneticPMID:22681890
SPBP8B7.10cU3 snoRNP-associated protein Utp16 (predicted) Negative GeneticPMID:22681890
vht1vitamin H transporter Vht1 Negative GeneticPMID:22681890
SPBP16F5.05cribosome biogenesis protein Nop8 (predicted) Positive GeneticPMID:22681890
hip4histone promoter control protein Hip4 Negative GeneticPMID:18818364
hhf1histone H4 h4.1 Negative GeneticPMID:22681890
bob1prefoldin subunit 5 (predicted) Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
idp1isocitrate dehydrogenase Idp1 (predicted) Negative GeneticPMID:22681890
ubc6ubiquitin conjugating enzyme Ubc6 (predicted) Negative GeneticPMID:18818364
fkbp39FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) Positive GeneticPMID:18818364
Positive GeneticPMID:22681890
eme1Holliday junction resolvase subunit Eme1 Negative GeneticPMID:18818364
Positive GeneticPMID:22681890
prw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
rps00140S ribosomal protein S0A (p40) Positive GeneticPMID:22681890
rsc1RSC complex subunit Rsc1 Negative GeneticPMID:18818364
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
pex11peroxisomal biogenesis factor 11 (predicted) Positive GeneticPMID:22681890
rpl380160S ribosomal protein L38 (predicted) Positive GeneticPMID:22681890
gcd10tRNA (m1A) methyltransferase Gcd10 (predicted) Positive GeneticPMID:22681890
sum2G2/M transition checkpoint protein Sum2 Negative GeneticPMID:22681890
ams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
abc2glutathione S-conjugate-exporting ATPase Abc2 Negative GeneticPMID:22681890
grx5monothiol glutaredoxin Grx5 Negative GeneticPMID:22681890
ace2transcription factor Ace2 Negative GeneticPMID:22681890
SPAC1610.02cmitochondrial ribosomal protein subunit L1 (predicted) Positive GeneticPMID:22681890
ago1argonaute Negative GeneticPMID:22681890
elp4elongator complex subunit Elp4 (predicted) Positive GeneticPMID:18818364
efc25Ras1 guanyl-nucleotide exchange factor Efc25 Negative GeneticPMID:22681890
SPAC30C2.04cofactor for methionyl-and glutamyl-tRNA synthetases (predicted) Positive GeneticPMID:22681890
zds1zds family protein phosphatase type A regulator Zds1 (predicted) Negative GeneticPMID:18818364
raf2Rik1-associated factor Raf2 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
SPBC17A3.06phosphoprotein phosphatase (predicted) Positive GeneticPMID:22681890
moe1translation initiation factor eIF3d Moe1 Positive GeneticPMID:22681890
dph1UBA domain protein Dph1 Negative GeneticPMID:18818364
SPAC26A3.11amidohydrolase (predicted) Negative GeneticPMID:22681890
pds5cohesin-associated protein Pds5 Negative GeneticPMID:18818364
cch1calcium channel Cch1 Positive GeneticPMID:22681890
elp6elongator complex subunit Elp6 (predicted) Positive GeneticPMID:18818364
SPBC16A3.10membrane bound O-acyltransferase, MBOAT (predicted) Negative GeneticPMID:22681890
vps38phophatidylinositol 3-kinase complex subunit Vps38 Positive GeneticPMID:22681890
dot2ESCRT II complex subunit Dot2 Positive GeneticPMID:18818364
clr1cryptic loci regulator Clr1 Negative GeneticPMID:22681890
atp11F1-ATPase chaperone Atp11 (predicted) Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
bdf2BET family double bromodomain protein Bdf2 Negative GeneticPMID:18818364
SPAPB1E7.11csequence orphan Positive GeneticPMID:22681890
pht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:18818364
trt1telomerase reverse transcriptase 1 protein Trt1 Negative GeneticPMID:22681890
srb11cyclin CycC, Srb mediator subunit Srb11 Negative GeneticPMID:18818364
rps120140S ribosomal protein S12 (predicted) Positive GeneticPMID:22681890
arp6actin-like protein Arp6 Negative GeneticPMID:18818364
lys2homoaconitate hydratase Lys2 Negative GeneticPMID:22681890
rdp1RNA-directed RNA polymerase Rdp1 Negative GeneticPMID:18818364
swi2Swi5 complex subunit Swi2 Negative GeneticPMID:18818364
ase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:18818364
rps280240S ribosomal protein S28, Rps2802 Positive GeneticPMID:22681890
set1histone lysine methyltransferase Set1 Negative GeneticPMID:18818364
iwr1RNA polymerase II nuclear import protein Iwr1 (predicted) Positive GeneticPMID:18818364
Positive GeneticPMID:22681890
clr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
cdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
rpa12DNA-directed RNA polymerase complex I subunit Rpa12 Negative GeneticPMID:18818364
pek1MAP kinase kinase Pek1 Negative GeneticPMID:22681890
cho1phosphatidyl-N-methylethanolamine N-methyltransferase (predicted) Negative GeneticPMID:18818364
acp2F-actin capping protein beta subunit Acp2 Negative GeneticPMID:22681890
rad2514-3-3 protein Rad25 Negative GeneticPMID:18818364
rpl70260S ribosomal protein L7 Positive GeneticPMID:22681890
nrm1MBF complex corepressor Nrm1 Negative GeneticPMID:22681890
not2CCR4-Not complex subunit Not2 (predicted) Positive GeneticPMID:18818364
ppr5mitochondrial PPR repeat protein Ppr5 Negative GeneticPMID:22681890
tom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
rpl160160S ribosomal protein L13/L16 (predicted) Positive GeneticPMID:18818364
Positive GeneticPMID:22681890
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:22681890
rps160240S ribosomal protein S16 (predicted) Positive GeneticPMID:22681890
rps170240S ribosomal protein S17 (predicted) Positive GeneticPMID:22681890
omh6alpha-1,2-mannosyltransferase Omh6 (predicted) Negative GeneticPMID:22681890
sec28coatomer epsilon subunit (predicted) Negative GeneticPMID:18818364
SPBC365.16conserved protein Negative GeneticPMID:22681890
spf1Set1C PHD Finger protein Spf1 Negative GeneticPMID:18818364
dsc5UBX domain containing protein required for Sre1 cleavage Negative GeneticPMID:22681890
rpl90160S ribosomal protein L9 Positive GeneticPMID:22681890
ppk26protein kinase like PAN complex subunit Ppk26 Negative GeneticPMID:22681890
snr13-hydroxyisobutyryl-CoA hydrolase snr1 Positive GeneticPMID:22681890
SPAC3A11.13prefoldin subunit 6 (predicted) Negative GeneticPMID:18818364
vps71Swr1 complex subunit Vps71 Negative GeneticPMID:18818364
coq10mitochondrial ubiquinone binding protein Coq10 Negative GeneticPMID:22681890
rad8ubiquitin-protein ligase E3 Rad8 Negative GeneticPMID:18818364
cbp1CENP-B homolog Negative GeneticPMID:18818364
scs7sphingosine hydroxylase Scs7 Negative GeneticPMID:18818364
rps2340S ribosomal protein S23 (predicted) Positive GeneticPMID:22681890
SPAC22H10.09sequence orphan Negative GeneticPMID:22681890
set3histone lysine methyltransferase Set3 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
swd3WD repeat protein Swd3 Negative GeneticPMID:18818364
cmk2MAPK-activated protein kinase Cmk2 Negative GeneticPMID:22681890
SPBC725.01aspartate aminotransferase (predicted) Negative GeneticPMID:22681890
nem1Nem1-Spo7 phosphatase complex catalytic subunit Nem1 (predicted) Negative GeneticPMID:22681890
pmk1MAP kinase Pmk1 Negative GeneticPMID:18818364
mug178mitochondrial ribosomal protein subunit L51-b (predicted) Negative GeneticPMID:22681890
mug154conserved fungal protein Negative GeneticPMID:22681890
dsk1SR protein-specific kinase Dsk1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
bun62WD repeat protein, human WDR20 family, Bun62 Negative GeneticPMID:22681890
rsv2transcription factor Rsv2 Negative GeneticPMID:18818364
rpl190260S ribosomal protein L19 Positive GeneticPMID:22681890
ppr2mitochondrial PPR repeat protein Ppr2 Positive GeneticPMID:22681890
atp12F1-ATPase chaperone Atp12 (predicted) Negative GeneticPMID:22681890
cay1cactin, spliceosome complex subunit (predicted) Positive GeneticPMID:18818364
rpl210260S ribosomal protein L21 (predicted) Positive GeneticPMID:22681890
psh3ER chaperone SHR3 homologue Psh3 Positive GeneticPMID:22681890
ptp4phosphatidate cytidylyltransferase Ptp4 (predicted) Negative GeneticPMID:22681890
res1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
raf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Negative GeneticPMID:22681890
srs2ATP-dependent DNA helicase, UvrD subfamily Negative GeneticPMID:18818364
puf6Puf family RNA-binding protein Puf6 (predicted) Positive GeneticPMID:22681890
dcr1dicer Negative GeneticPMID:22681890
rpl230160S ribosomal protein L23 Positive GeneticPMID:22681890
rps40140S ribosomal protein S4 (predicted) Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC22F3.08c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC22F3.08c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC22F3.08c BioGRID Interaction Datasets
Expression Viewer SPAC22F3.08c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC22F3.08c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC22F3.08c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC22F3.08c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC22F3.08c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC22F3.08c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC22F3.08c Cell Cycle Data
GEO SPAC22F3.08c GEO profiles
PInt SPAC22F3.08c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2541786ATP-dependent RNA helicase Rok1 (predicted)
EntrezGene2541786ATP-dependent RNA helicase Rok1 (predicted)
SPD / RIKEN23/23C07Orfeome Localization Data
UniProtKB/SwissProtQ09775ATP-dependent RNA helicase rok1
ModBaseQ09775Database of comparative protein structure models
STRINGQ09775Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593033ATP-dependent RNA helicase Rok1 (predicted)
RefSeq mRNANM_001018432972h- ATP-dependent RNA helicase Rok1 (predicted) (rok1), mRNA
European Nucleotide ArchiveCAA91073ENA Protein Mapping
European Nucleotide ArchiveCAA91073.1ENA Protein Mapping
UniParcUPI0000139FCAUniProt Archive

Literature for rok1

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014