pam1 (SPAC22F8.06)


Gene Standard Namepam1 Characterisation Statuspublished
Systematic IDSPAC22F8.06 Feature Typeprotein coding
Synonyms Name Description
Product20S proteasome complex subunit beta 6 Product Size225aa, 25.08 kDa
Genomic Location Chromosome I, 4795276-4796348 (1073nt); CDS:4795479-4796156 (678nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004175endopeptidase activity66
contributes_toISSUniProtKB:P23724GO_REF:0000001
GO:0004298threonine-type endopeptidase activityIEAUniProtKB-KW:KW-0888GO_REF:000003715
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0043161proteasome-mediated ubiquitin-dependent protein catabolic processISSUniProtKB:P23724GO_REF:0000001110
GO:0007346regulation of mitotic cell cycleNASGO_REF:0000051281
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722316
GO:0005634nucleusIDAPMID:168233722740
GO:0019774proteasome core complex, beta-subunit complexISSUniProtKB:P23724GO_REF:00000017
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopypam1ΔNullPECO:0000005, PECO:0000137PMID:236978061331
Microscopypam1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000311inviable after spore germination with normal, unseptated germ tube morphologyMicroscopypam1ΔNullPECO:0000005, PECO:0000137PMID:23697806237
FYPO:0002151inviable spore459
penetrance FYPO_EXT:0000001Microscopypam1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
147952764796348

UTRs

Region Coordinates Reference
five_prime_UTR4795276..4795478PMID:21511999
three_prime_UTR4796157..4796348PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00227 Pfam IPR001353 Proteasome, subunit alpha/beta 10 197 14
PS00854 Prosite Patterns IPR016050 Proteasome beta-type subunit, conserved site 17 64 8
PS51476 Prosite Profiles IPR023333 Proteasome B-type subunit 13 210 7
PTHR11599 HMMPANTHER 1 225 14
PTHR11599:SF52 HMMPANTHER 1 225 1
3.60.20.10 Gene3D IPR029055 Nucleophile aminohydrolases, N-terminal 6 225 19
SSF56235 SuperFamily IPR029055 Nucleophile aminohydrolases, N-terminal 8 221 22

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.48 Da
Charge 3.00
Isoelectric point 7.59
Molecular weight 25.08 kDa
Number of residues 225
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
141716during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
167710during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
159821during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
157506during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
24122.26during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
164405during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
17772.41during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
4.8during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
0.82during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3393
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
uch2ubiquitin C-terminal hydrolase Uch2 Affinity Capture-MSPMID:20838651
rpn1119S proteasome regulatory subunit Rpn11 Affinity Capture-MSPMID:20838651
External References
Database Identifier Description
NBRP SPAC22F8.06 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC22F8.06 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC22F8.06 BioGRID Interaction Datasets
Expression Viewer SPAC22F8.06 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC22F8.06 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC22F8.06 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC22F8.06 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC22F8.06 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC22F8.06 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC22F8.06 Cell Cycle Data
GEO SPAC22F8.06 GEO profiles
PInt SPAC22F8.06 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC22F8.06 Peptides identified in tandem mass spectrometry proteomics experiments
WikiGene254206220S proteasome component beta 6
EntrezGene254206220S proteasome component beta 6
IntEnz3.4.25.1Integrated relational Enzyme database
Rhea3.4.25.1Annotated reactions database
SPD / RIKEN42/42H07Orfeome Localization Data
UniProtKB/SwissProtQ9UQY2Probable proteasome subunit beta type-6
ModBaseQ9UQY2Database of comparative protein structure models
STRINGQ9UQY2Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_59472920S proteasome component beta 6
RefSeq mRNANM_001020157972h- 20S proteasome component beta 6 (pam1), mRNA
MEROPST01.986
European Nucleotide ArchiveBAA88692ENA Protein Mapping
European Nucleotide ArchiveBAA88692.1ENA Protein Mapping
European Nucleotide ArchiveCAB52716ENA Protein Mapping
European Nucleotide ArchiveCAB52716.1ENA Protein Mapping
ReactomeREACT_188524
ReactomeREACT_188566
ReactomeREACT_188568
ReactomeREACT_188587
ReactomeREACT_208775
ReactomeREACT_215140
ReactomeREACT_215320
ReactomeREACT_218991
ReactomeREACT_81558
UniParcUPI00001325A0UniProt Archive

Literature for pam1

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014