pam1 (SPAC22F8.06)


Gene Standard Namepam1 Characterisation Statuspublished
Systematic IDSPAC22F8.06 Feature Typeprotein coding
Synonyms Name Description
Product20S proteasome complex subunit beta 6 Product Size225aa, 25.08 kDa
Genomic Location Chromosome I, 4795276-4796348 (1073nt); CDS:4795479-4796156 (678nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004175endopeptidase activity66
contributes_toISSUniProtKB:P23724GO_REF:0000001
GO:0004298threonine-type endopeptidase activityIEAUniProtKB-KW:KW-0888GO_REF:000003715
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0043161proteasome-mediated ubiquitin-dependent protein catabolic processISSUniProtKB:P23724GO_REF:0000001112
GO:0007346regulation of mitotic cell cycleNASGO_REF:0000051280
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722319
GO:0005634nucleusIDAPMID:168233722737
GO:0019774proteasome core complex, beta-subunit complexISSUniProtKB:P23724GO_REF:00000017
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopypam1ΔNullPECO:0000005, PECO:0000137PMID:236978061337
Microscopypam1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000311inviable after spore germination with normal, unseptated germ tube morphologyMicroscopypam1ΔNullPECO:0000005, PECO:0000137PMID:23697806237
FYPO:0002151inviable spore462
penetrance FYPO_EXT:0000001Microscopypam1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
147952764796348
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00227 Pfam IPR001353 Proteasome, subunit alpha/beta 10 197 14
PS00854 Prosite Patterns IPR016050 Proteasome beta-type subunit, conserved site 17 64 8
PS51476 Prosite Profiles IPR023333 Proteasome B-type subunit 13 210 7
PTHR11599 HMMPANTHER 1 225 14
PTHR11599:SF8 HMMPANTHER 1 225 1
3.60.20.10 Gene3D 6 225 19
SSF56235 SuperFamily Nucleophile aminohydrolases, N-terminal 8 221 22

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.48 Da
Charge 3.00
Isoelectric point 7.59
Molecular weight 25.08 kDa
Number of residues 225
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
141716during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
167710during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
159821during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
157506during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
164405during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
24122.26during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
17772.41during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.8during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.82during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in bacteria1002
conserved in metazoa3428
conserved in vertebrates3402
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
uch2ubiquitin C-terminal hydrolase Uch2 Affinity Capture-MSPMID:20838651
rpn1119S proteasome regulatory subunit Rpn11 Affinity Capture-MSPMID:20838651
External References
Database Identifier Description
NBRP SPAC22F8.06 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC22F8.06 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC22F8.06 BioGRID Interaction Datasets
Expression Viewer SPAC22F8.06 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC22F8.06 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC22F8.06 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC22F8.06 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC22F8.06 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC22F8.06 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC22F8.06 Cell Cycle Data
GEO SPAC22F8.06 GEO profiles
PInt SPAC22F8.06 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC22F8.06 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN42/42H07Orfeome Localization Data
IntEnz3.4.25.1Integrated relational Enzyme database
Rhea3.4.25.1Annotated reactions database
UniProtKB/SwissProtQ9UQY2Probable proteasome subunit beta type-6
ModBaseQ9UQY2Database of comparative protein structure models
STRINGQ9UQY2Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_59472920S proteasome component beta 6
RefSeq mRNANM_001020157972h- 20S proteasome component beta 6 (pam1), mRNA
European Nucleotide ArchiveBAA88692.1ENA Protein Mapping
European Nucleotide ArchiveCAB52716.1ENA Protein Mapping
UniParcUPI00001325A0UniProt Archive

Literature for pam1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014