pam1 (SPAC22F8.06)


Gene Standard Namepam1 Characterisation Statuspublished
Systematic IDSPAC22F8.06 Feature Typeprotein coding
Synonyms Name Description
Product20S proteasome complex subunit beta 6 Product Size225aa, 25.08 kDa
Genomic Location Chromosome I, 4795276-4796348 (1073nt); CDS:4795479-4796156 (678nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
endopeptidase activity65
Annotation ExtensionEvidenceWith/FromReference
threonine-type endopeptidase activity15
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
positive regulation of mitotic metaphase/anaphase transition53
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
proteasome core complex, beta-subunit complex7
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationpam1Δ1450

Cell Phenotype

Term NameGenotypesCount
inviable after spore germination with normal, unseptated germ tube morphologypam1Δ237
inviable sporepam1Δ475
Target Of
OntologyRelationshipGeneProduct
GO substrate of ubp15 ubiquitin C-terminal hydrolase Ubp15
GO substrate of ubp4 ubiquitin C-terminal hydrolase Ubp4
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4795276..4796348
5' UTR4795276..4795478PMID:21511999
3' UTR4796157..4796348PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00227 Pfam IPR001353 Proteasome, subunit alpha/beta 10 197 14
PS51476 Prosite Profiles IPR023333 Proteasome B-type subunit 13 210 7
PS00854 Prosite Patterns IPR016050 Proteasome beta-type subunit, conserved site 17 64 8
PTHR11599 HMMPANTHER 3 225 14
PTHR11599:SF59 HMMPANTHER 3 225 1
3.60.20.10 Gene3D IPR029055 Nucleophile aminohydrolases, N-terminal 6 225 19
SSF56235 SuperFamily IPR029055 Nucleophile aminohydrolases, N-terminal 8 221 22

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.48 Da
Charge 3.00
Codon Adaptation Index 0.45
Isoelectric point 7.59
Molecular weight 25.08 kDa
Number of residues 225
Modifications

Protein Modifications

Term NameResidueCount
ubiquitinylated lysineK70, K223 512
Annotation ExtensionEvidenceResidueReference
IDA PMID:26412298
mass spectrometry evidence K70 PMID:26412298
mass spectrometry evidence K223 PMID:26412298
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for pam1 (SPAC22F8.06)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
141716during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
167710during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
159821during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
157506during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
164405during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
24122.26during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
17772.41during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.8during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.82during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3090
conserved in fungi4604
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3422
conserved in vertebrates3397
conserved in archaea238
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load gene that interact physically with SPAC22F8.06 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bypre620S proteasome complex subunit alpha 4 Pre6 Affinity Capture-MSPMID:20133687
affinity captured byrpn1119S proteasome regulatory subunit Rpn11 Affinity Capture-MSPMID:20838651
affinity captured byuch2ubiquitin C-terminal hydrolase Uch2 Affinity Capture-MSPMID:20838651
External References
Database Identifier Description
NBRP SPAC22F8.06 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC22F8.06 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC22F8.06 BioGRID Interaction Datasets
Expression Viewer SPAC22F8.06 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC22F8.06 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC22F8.06 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC22F8.06 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC22F8.06 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC22F8.06 Transcriptome Viewer (Bähler Lab)
GEO SPAC22F8.06 GEO profiles
PInt SPAC22F8.06 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC22F8.06 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC22F8.06 Fission yeast phenotypic data & analysis
Cyclebase SPAC22F8.06.1 Cell Cycle Data
SPD / RIKEN42/42H07Orfeome Localization Data
IntEnz3.4.25.1Integrated relational Enzyme database
Rhea3.4.25.1Annotated reactions database
UniProtKB/SwissProtQ9UQY2Probable proteasome subunit beta type-6
ModBaseQ9UQY2Database of comparative protein structure models
STRINGQ9UQY2Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_59472920S proteasome component beta 6
RefSeq mRNANM_001020157972h- 20S proteasome component beta 6 (pam1), mRNA
European Nucleotide ArchiveAB012135ENA EMBL mapping
European Nucleotide ArchiveCU329670ENA EMBL mapping
MEROPST01.A12MEROPS - the Peptidase Database
European Nucleotide ArchiveBAA88692ENA Protein Mapping
European Nucleotide ArchiveCAB52716ENA Protein Mapping
UniParcUPI00001325A0UniProt Archive

Literature for pam1

Search: Europe PMC or PubMed

Release Version: PomBase:30_57 - 27 Jan 2016