pam1 (SPAC22F8.06)


Gene Standard Namepam1 Characterisation Statuspublished
Systematic IDSPAC22F8.06 Feature Typeprotein coding
Synonyms Name Description
Product20S proteasome complex subunit beta 6 Product Size225aa, 25.08 kDa
Genomic Location Chromosome I, 4795276-4796348 (1073nt); CDS:4795479-4796156 (678nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004175endopeptidase activity68
contributes_toISSUniProtKB:P23724GO_REF:0000001
GO:0004298threonine-type endopeptidase activityIEAUniProtKB-KW:KW-0888GO_REF:000003715
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0043161proteasome-mediated ubiquitin-dependent protein catabolic processISSUniProtKB:P23724GO_REF:0000001106
GO:0007346regulation of mitotic cell cycleNASGO_REF:0000051270
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722317
GO:0005634nucleusIDAPMID:168233722730
GO:0019774proteasome core complex, beta-subunit complexISSUniProtKB:P23724GO_REF:00000017
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopypam1ΔNullPECO:0000005, PECO:0000137PMID:236978061309
Microscopypam1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000311inviable after spore germination with normal, unseptated germ tube morphologyMicroscopypam1ΔNullPECO:0000005, PECO:0000137PMID:23697806237
FYPO:0002151inviable sporeMicroscopypam1ΔNullPECO:0000005, PECO:0000137PMID:23697806447
penetrance FYPO_EXT:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
147952764796348

UTRs

Region Start End Reference
three_prime_UTR47961574796348PMID:21511999
five_prime_UTR47952764795478PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00227 Pfam IPR001353 Proteasome, subunit alpha/beta 10 197 14
PS51476 Prosite Profiles IPR023333 Proteasome B-type subunit 13 210 7
PS00854 Prosite Patterns IPR016050 Proteasome beta-type subunit, conserved site 17 64 8
PTHR11599:SF8 HMMPANTHER 1 225 1
PTHR11599 HMMPANTHER 1 225 14
G3DSA:3.60.20.10 Gene3D 6 225 19
SSF56235 SuperFamily 8 221 22

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.48 Da
Charge 3.00
Isoelectric point 7.59
Molecular weight 25.08 kDa
Number of residues 225
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
17772.41during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
24122.26during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
4.8during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.82during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3394
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
uch2ubiquitin C-terminal hydrolase Uch2 Affinity Capture-MSPMID:20838651
rpn1119S proteasome regulatory subunit Rpn11 Affinity Capture-MSPMID:20838651
External References
Database Identifier Description
NBRP SPAC22F8.06 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC22F8.06 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC22F8.06 BioGRID Interaction Datasets
Expression Viewer SPAC22F8.06 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC22F8.06 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC22F8.06 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC22F8.06 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC22F8.06 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC22F8.06 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC22F8.06 Cell Cycle Data
GEO SPAC22F8.06 GEO profiles
PInt SPAC22F8.06 Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene254206220S proteasome component beta 6
EntrezGene254206220S proteasome component beta 6
IntEnz3.4.25.1Integrated relational Enzyme database
Rhea3.4.25.1Annotated reactions database
SPD / RIKEN42/42H07Orfeome Localization Data
UniProtKB/SwissProtQ9UQY2Probable proteasome subunit beta type-1
ModBaseQ9UQY2Database of comparative protein structure models
StringQ9UQY2Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_59472920S proteasome component beta 6
RefSeq mRNANM_001020157972h- 20S proteasome component beta 6 (pam1), mRNA
MEROPST01.986
European Nucleotide ArchiveBAA88692ENA Protein Mapping
European Nucleotide ArchiveCAB52716ENA Protein Mapping
UniParcUPI00001325A0UniProt Archive

Literature for pam1

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014