kap114 (SPAC22H10.03c)


Gene Standard Namekap114 Characterisation Statuspublished
Systematic IDSPAC22H10.03c Feature Typeprotein coding
Synonyms Name Description
Productkaryopherin Kap14 Product Size993aa, 111.86 kDa
Genomic Location Chromosome I, 2376572-2373052 (3521nt); CDS:2376385-2373144 (3242nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005525GTP bindingNASGO_REF:0000001113
GO:0008536Ran GTPase bindingIEAIPR001494GO_REF:000000215
GO:0008565protein transporter activityTASPMID:1511643248
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006913nucleocytoplasmic transportNASGO_REF:0000051112
GO:0006605protein targetingNASGO_REF:0000051164
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722319
GO:0005635nuclear envelopeIDAPMID:16823372137
GO:0005643nuclear poreNASGO_REF:000005154
GO:0005634nucleusIDAPMID:168233722737
IDAPMID:15116432
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopykap114ΔNullPMID:204732893751
Microscopykap114ΔNullPMID:15116432
Microscopykap114ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopykap114ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
123765722376280
223762232374002
323739612373792
423737442373626
523735832373413
623733762373303
723732632373052
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03810 Pfam IPR001494 Importin-beta, N-terminal domain 24 100 8
SM00913 SMART IPR001494 Importin-beta, N-terminal domain 24 100 8
PS50166 Prosite Profiles IPR001494 Importin-beta, N-terminal domain 24 100 8
PTHR10997:SF9 HMMPANTHER 1 990 1
PTHR10997 HMMPANTHER 1 990 4
1.25.10.10 Gene3D IPR011989 Armadillo-like helical 5 848 86
1.25.10.10 Gene3D IPR011989 Armadillo-like helical 945 989 86
1.25.10.10 Gene3D IPR011989 Armadillo-like helical 880 891 86
SSF48371 SuperFamily IPR016024 Armadillo-type fold 5 989 130

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000561importin familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000561

Protein Properties

Ave. residue weight 112.65 Da
Charge -66.00
Isoelectric point 4.19
Molecular weight 111.86 kDa
Number of residues 993
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
6581during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
6490during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
7054during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
6438during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
6832during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
6390.62during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
2356.72during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.9during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.56during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999432
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
pht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:18818364
srb11cyclin CycC, Srb mediator subunit Srb11 Positive GeneticPMID:22681890
duo1DASH complex subunit Duo1 Negative GeneticPMID:22681890
lub1WD repeat protein Lub1 Positive GeneticPMID:18818364
prp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
mph1dual specificity protein kinase Mph1 Positive GeneticPMID:22681890
SPAC23H4.16cSchizosaccharomyces specific protein Negative GeneticPMID:22681890
git5heterotrimeric G protein beta subunit Git5 Negative GeneticPMID:22681890
hip4histone promoter control protein Hip4 Negative GeneticPMID:18818364
sds23PP2A-type phosphatase inhibitor Sds23/Moc1 Negative GeneticPMID:22681890
cho1phosphatidyl-N-methylethanolamine N-methyltransferase (predicted) Positive GeneticPMID:18818364
SPCC63.03DNAJ domain protein, DNAJC11 family Positive GeneticPMID:22681890
mms19Dos2 silencing complex subunit Mms19 Negative GeneticPMID:18818364
SPAC3A12.13ctranslation initiation factor eIF3j (p35) Negative GeneticPMID:22681890
atp14F1-ATPase subunit H (predicted) Positive GeneticPMID:22681890
tim13TIM22 inner membrane protein import complex subunit Tim13 (predicted) Negative GeneticPMID:18818364
SPAC13G6.09SSU-rRNA maturation protein Tsr4 homolog 2 (predicted) Negative GeneticPMID:22681890
mpr1histidine-containing response regulator phosphotransferase Mpr1 Negative GeneticPMID:22681890
SPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:22681890
dad4DASH complex subunit Dad4 Negative GeneticPMID:22681890
spc19DASH complex subunit Spc19 Negative GeneticPMID:22681890
dad3DASH complex subunit Dad3 Negative GeneticPMID:22681890
mug154conserved fungal protein Negative GeneticPMID:22681890
swi3replication fork protection complex subunit Swi3 Negative GeneticPMID:18818364
sck2serine/threonine protein kinase Sck2 Positive GeneticPMID:24463365
pmc5mediator complex subunit Pmc5 Negative GeneticPMID:18818364
rho3Rho family GTPase Rho3 Positive GeneticPMID:22681890
med7mediator complex subunit Med7 Negative GeneticPMID:22681890
pob3FACT complex subunit Pob3 Negative GeneticPMID:18818364
amo1nuclear rim protein Amo1 Positive GeneticPMID:18818364
ptn1phosphatidylinositol-3,4,5-trisphosphate3-phosphatase Ptn1 Negative GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Positive GeneticPMID:22681890
rpl160160S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:22681890
cph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
sts5RNB-like protein Positive GeneticPMID:22681890
SPBC1861.07elongin C (predicted) Negative GeneticPMID:22681890
hrp3ATP-dependent DNA helicase Hrp3 Positive GeneticPMID:22681890
ser2phosphoserine phosphatase Ser2 (predicted) Negative GeneticPMID:18818364
psh3ER chaperone SHR3 homologue Psh3 Negative GeneticPMID:22681890
cxr1mRNA processing factor Positive GeneticPMID:22681890
vps901guanyl-nucleotide exchange factor Vps901 (predicted) Negative GeneticPMID:22681890
SPBP35G2.14RNA-binding protein Negative GeneticPMID:22681890
fkbp39FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) Negative GeneticPMID:18818364
SPAC31A2.12arrestin/PY protein 1 (predicted) Negative GeneticPMID:22681890
SPBC36B7.08cnucleosome assembly protein (predicted) Negative GeneticPMID:18818364
alp14TOG ortholog Alp14 Negative GeneticPMID:18818364
snr13-hydroxyisobutyryl-CoA hydrolase snr1 Positive GeneticPMID:22681890
sap114U2 snRNP subunit Sap114 Negative GeneticPMID:22681890
sde2silencing defective protein Sde2 Negative GeneticPMID:22681890
ryh1GTPase Ryh1 Negative GeneticPMID:18818364
SPAP8A3.07cphospho-2-dehydro-3-deoxyheptonate aldolase (predicted) Negative GeneticPMID:22681890
gsk3serine/threonine protein kinase Gsk3 Negative GeneticPMID:22681890
SPAC10F6.13caspartate aminotransferase (predicted) Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC22H10.03c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC22H10.03c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC22H10.03c BioGRID Interaction Datasets
Expression Viewer SPAC22H10.03c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC22H10.03c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC22H10.03c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC22H10.03c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC22H10.03c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC22H10.03c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC22H10.03c Cell Cycle Data
GEO SPAC22H10.03c GEO profiles
PInt SPAC22H10.03c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC22H10.03c Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN48/48B05Orfeome Localization Data
UniProtKB/SwissProtQ10297Importin subunit beta-5
ModBaseQ10297Database of comparative protein structure models
STRINGQ10297Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593739karyopherin Kap14
RefSeq mRNANM_001019170972h- karyopherin Kap14 (kap114), mRNA
European Nucleotide ArchiveCAA93604.2ENA Protein Mapping
UniParcUPI000059CE02UniProt Archive

Literature for kap114

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014