kap114 (SPAC22H10.03c)


Gene Standard Namekap114 Characterisation Statuspublished
Systematic IDSPAC22H10.03c Feature Typeprotein coding
Synonyms Name Description
Productkaryopherin Kap14 Product Size993aa, 111.86 kDa
Genomic Location Chromosome I, 2376572-2373052 (3521nt); CDS:2376385-2373144 (3242nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
GTP binding113
Annotation ExtensionEvidenceWith/FromReference
Ran GTPase binding15
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
nucleocytoplasmic transport112
Annotation ExtensionEvidenceWith/FromReference
protein targeting170
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
nuclear envelope133
Annotation ExtensionEvidenceWith/FromReference
nuclear pore52
Annotation ExtensionEvidenceWith/FromReference
nucleus2695
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
viable vegetative cell populationkap114ΔNull3795

Cell Phenotype

Term NameAlleleExpressionCount
normal protein import into nucleuskap114ΔNull15
viable vegetative cell with normal cell morphologykap114ΔNull3093
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2376572..2376280, 2376223..2374002, 2373961..2373792, 2373744..2373626, 2373583..2373413, 2373376..2373303, 2373263..2373052
mRNA2376572..2373052
5' UTR2376572..2376386PMID:21511999
CDS2376385..2376280, 2376223..2374002, 2373961..2373792, 2373744..2373626, 2373583..2373413, 2373376..2373303, 2373263..2373144
3' UTR2373143..2373052AU010623
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03810 Pfam IPR001494 Importin-beta, N-terminal domain 24 100 8
SM00913 SMART IPR001494 Importin-beta, N-terminal domain 24 100 8
PS50166 Prosite Profiles IPR001494 Importin-beta, N-terminal domain 24 100 8
PTHR10997:SF9 HMMPANTHER 1 990 1
PTHR10997 HMMPANTHER 1 990 4
1.25.10.10 Gene3D IPR011989 Armadillo-like helical 945 989 86
1.25.10.10 Gene3D IPR011989 Armadillo-like helical 880 891 86
1.25.10.10 Gene3D IPR011989 Armadillo-like helical 5 848 86
SSF48371 SuperFamily IPR016024 Armadillo-type fold 5 989 130

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000561importin familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000561

Protein Properties

Ave. residue weight 112.65 Da
Charge -66.00
Isoelectric point 4.19
Molecular weight 111.86 kDa
Number of residues 993
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
6581during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
6490during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
7054during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
6438during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
6832during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
6390.62during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
2356.72during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.9during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.56during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999432
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withalp14TOG/XMAP14 family protein Alp14 Negative GeneticPMID:18818364
positive genetic interaction withamo1nuclear rim protein Amo1 Positive GeneticPMID:18818364
positive genetic interaction withatp14F1-ATPase subunit H (predicted) Positive GeneticPMID:22681890
positive genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withcho1phosphatidyl-N-methylethanolamine N-methyltransferase (predicted) Positive GeneticPMID:18818364
negative genetic interaction withcph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
positive genetic interaction withcxr1mRNA processing factor Positive GeneticPMID:22681890
negative genetic interaction withdad3DASH complex subunit Dad3 Negative GeneticPMID:22681890
negative genetic interaction withdad4DASH complex subunit Dad4 Negative GeneticPMID:22681890
negative genetic interaction withduo1DASH complex subunit Duo1 Negative GeneticPMID:22681890
negative genetic interaction withfkbp39FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) Negative GeneticPMID:18818364
negative genetic interaction withgit5heterotrimeric G protein beta subunit Git5 Negative GeneticPMID:22681890
negative genetic interaction withgsk3serine/threonine protein kinase Gsk3 Negative GeneticPMID:22681890
negative genetic interaction withhcr1translation initiation factor eIF3j (p35) Negative GeneticPMID:22681890
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:18818364
positive genetic interaction withhrp3ATP-dependent DNA helicase Hrp3 Positive GeneticPMID:22681890
positive genetic interaction withlub1WD repeat protein Lub1 Positive GeneticPMID:18818364
negative genetic interaction withmed6mediator complex subunit Med6 Negative GeneticPMID:18818364
negative genetic interaction withmed7mediator complex subunit Med7 Negative GeneticPMID:22681890
negative genetic interaction withmms19Dos2 silencing complex subunit Mms19 Negative GeneticPMID:18818364
positive genetic interaction withmph1dual specificity protein kinase Mph1 Positive GeneticPMID:22681890
negative genetic interaction withmpr1histidine-containing response regulator phosphotransferase Mpr1 Negative GeneticPMID:22681890
negative genetic interaction withmug154conserved fungal protein Negative GeneticPMID:22681890
negative genetic interaction withpht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:18818364
negative genetic interaction withpob3FACT complex subunit Pob3 Negative GeneticPMID:18818364
negative genetic interaction withprp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
negative genetic interaction withpsh3ER chaperone SHR3 homologue Psh3 Negative GeneticPMID:22681890
negative genetic interaction withptn1phosphatidylinositol-3,4,5-trisphosphate3-phosphatase Ptn1 Negative GeneticPMID:22681890
positive genetic interaction withrho3Rho family GTPase Rho3 Positive GeneticPMID:22681890
negative genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withryh1GTPase Ryh1 Negative GeneticPMID:18818364
negative genetic interaction withsap114U2 snRNP subunit Sap114 Negative GeneticPMID:22681890
positive genetic interaction withsck2serine/threonine protein kinase Sck2 Positive GeneticPMID:24463365
negative genetic interaction withsde2silencing defective protein Sde2 Negative GeneticPMID:22681890
negative genetic interaction withsds23PP2A-type phosphatase inhibitor Sds23/Moc1 Negative GeneticPMID:22681890
negative genetic interaction withser2phosphoserine phosphatase Ser2 (predicted) Negative GeneticPMID:18818364
positive genetic interaction withsnr13-hydroxyisobutyryl-CoA hydrolase snr1 Positive GeneticPMID:22681890
negative genetic interaction withSPAC10F6.13caspartate aminotransferase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC13G6.09SSU-rRNA maturation protein Tsr4 homolog 2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC23H4.16cSchizosaccharomyces specific protein Negative GeneticPMID:22681890
negative genetic interaction withSPAC31A2.12arrestin/PY protein 1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAP8A3.07cphospho-2-dehydro-3-deoxyheptonate aldolase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:22681890
negative genetic interaction withSPBC1861.07elongin C (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC36B7.08cnucleosome assembly protein (predicted) Negative GeneticPMID:18818364
negative genetic interaction withSPBP35G2.14RNA-binding protein Negative GeneticPMID:22681890
negative genetic interaction withspc19DASH complex subunit Spc19 Negative GeneticPMID:22681890
positive genetic interaction withSPCC63.03DNAJ domain protein, DNAJC11 family Positive GeneticPMID:22681890
positive genetic interaction withsrb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Positive GeneticPMID:22681890
positive genetic interaction withsrb11cyclin CycC, Srb mediator subunit Srb11 Positive GeneticPMID:22681890
positive genetic interaction withsts5RNB-like protein Positive GeneticPMID:22681890
negative genetic interaction withswi3replication fork protection complex subunit Swi3 Negative GeneticPMID:18818364
negative genetic interaction withtim13TIM22 inner membrane protein import complex subunit Tim13 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withvps901guanyl-nucleotide exchange factor Vps901 (predicted) Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC22H10.03c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC22H10.03c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC22H10.03c BioGRID Interaction Datasets
Expression Viewer SPAC22H10.03c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC22H10.03c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC22H10.03c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC22H10.03c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC22H10.03c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC22H10.03c Transcriptome Viewer (Bähler Lab)
GEO SPAC22H10.03c GEO profiles
PInt SPAC22H10.03c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC22H10.03c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC22H10.03c Fission yeast phenotypic data & analysis
Cyclebase SPAC22H10.03c.1 Cell Cycle Data
SPD / RIKEN48/48B05Orfeome Localization Data
UniProtKB/SwissProtQ10297Importin subunit beta-5
ModBaseQ10297Database of comparative protein structure models
STRINGQ10297Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593739karyopherin Kap14
RefSeq mRNANM_001019170972h- karyopherin Kap14 (kap114), mRNA
European Nucleotide ArchiveCAA93604.2ENA Protein Mapping
UniParcUPI000059CE02UniProt Archive

Literature for kap114

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015