sld3 (SPAC24H6.06)

Gene Standard Namesld3 Characterisation Statuspublished
Systematic IDSPAC24H6.06 Feature Typeprotein coding
Synonymsmug175 Name DescriptionMeiotically Upregulated Gene
ProductDNA replication pre-initiation complex subunit Sld3 Product Size699aa, 79.81 kDa
Genomic Location Chromosome I, 478803-476474 (2330nt); CDS:478696-476549 (2148nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
chromatin binding33
Annotation ExtensionEvidenceWith/FromReference
protein binding831
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cellular protein localization550
Annotation ExtensionEvidenceWith/FromReference
meiotic cell cycle437
Annotation ExtensionEvidenceWith/FromReference
mitotic DNA replication initiation34
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
DNA replication preinitiation complex21
Annotation ExtensionEvidenceWith/FromReference
nuclear replication fork64
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopysld3ΔNullPMID:204732891338
Microscopysld3ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0001701sensitive to bortezomib256
expressivity FYPO_EXT:0000002Cell growth assaysld3ΔNullPECO:0000005, PECO:0000137PMID:21760946

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000311inviable after spore germination with normal, unseptated germ tube morphology237
penetrance FYPO_EXT:0000001Microscopysld3ΔNullPECO:0000005, PECO:0000137PMID:23697806
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08639 Pfam IPR013948 DNA replication regulator Sld3 211 639 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.18 Da
Charge 38.00
Isoelectric point 9.90
Molecular weight 79.81 kDa
Number of residues 699
Gene Expression

Qualitative Gene Expression

RNA levelincreased during GO:0051321experimental evidencePMID:16303567

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.4during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
0.43during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
gene structure updated432
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi only623
conserved in fungi4600
conserved in eukaryotes4514

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
cdc45DNA replication pre-initiation complex subunit Cdc45 Affinity Capture-WesternPMID:12006645
rad4BRCT domain protein Rad4 Two-hybridPMID:21945095
mcm6MCM complex subunit Mcm6 Affinity Capture-WesternPMID:12006645
mcm7MCM complex subunit Mcm7 Affinity Capture-WesternPMID:12006645
mcm2MCM complex subunit Mcm2 Two-hybridPMID:21593208
psf2GINS complex subunit Psf2 Two-hybridPMID:21593208
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
cdc45DNA replication pre-initiation complex subunit Cdc45 Dosage RescuePMID:12006645
cdc10MBF transcription factor complex subunit Cdc10 Positive GeneticPMID:22681890
prp4serine/threonine protein kinase Prp4 Positive GeneticPMID:22681890
atp11F1-ATPase chaperone Atp11 (predicted) Negative GeneticPMID:22681890
uap2U2 snRNP-associated protein Uap2 Positive GeneticPMID:22681890
psf3GINS complex subunit Psf3 Synthetic Growth DefectPMID:16990792
drc1replication preinitiation complex assembly protein Dosage RescuePMID:21593208
ask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Positive GeneticPMID:22681890
SPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
alp31tubulin specific chaperone cofactor A, Alp31 Negative GeneticPMID:22681890
dad2DASH complex subunit Dad2 Positive GeneticPMID:22681890
sif1Sad1 interacting factor 1 Negative GeneticPMID:22681890
dam1DASH complex subunit Dam1 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC24H6.06 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC24H6.06 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC24H6.06 BioGRID Interaction Datasets
Expression Viewer SPAC24H6.06 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC24H6.06 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC24H6.06 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC24H6.06 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC24H6.06 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC24H6.06 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC24H6.06 Cell Cycle Data
GEO SPAC24H6.06 GEO profiles
PInt SPAC24H6.06 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC24H6.06 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC24H6.06 Fission yeast phenotypic data & analysis
SPD / RIKEN37/37E10Orfeome Localization Data
UniProtKB/SwissProtQ09761DNA replication regulator sld3
ModBaseQ09761Database of comparative protein structure models
STRINGQ09761Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_592946DNA replication pre-initiation complex subunit Sld3
RefSeq mRNANM_001018347972h- DNA replication pre-initiation complex subunit Sld3 (sld3), mRNA
European Nucleotide ArchiveCAA90850.3ENA Protein Mapping
UniParcUPI00001798DEUniProt Archive

Literature for sld3

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014