sld3 (SPAC24H6.06)


Gene Standard Namesld3 Characterisation Statuspublished
Systematic IDSPAC24H6.06 Feature Typeprotein coding
Synonymsmug175 Name DescriptionMeiotically Upregulated Gene
ProductDNA replication pre-initiation complex subunit Sld3 Product Size699aa, 79.81 kDa
Genomic Location Chromosome I, 478803-476474 (2330nt); CDS:478696-476549 (2148nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005515protein bindingIPIcdc45PMID:12006645865
IPImcm6PMID:12006645
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:1902975DNA replication initiation involved in mitotic DNA replicationIGIcdc45PMID:1200664529
GO:0034613cellular protein localization541
localizes cdc45IMPPMID:12006645
GO:0007126meiotic nuclear divisionIEAUniProtKB-KW:KW-0469GO_REF:0000037330
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0031261DNA replication preinitiation complexIDAPMID:1134416621
GO:0043596nuclear replication forkICGO:0005656GO_REF:000000164
GO:0005634nucleusIDAPMID:168233722737
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopysld3ΔNullPECO:0000005, PECO:0000137PMID:236978061337
Microscopysld3ΔNullPMID:20473289
FYPO:0001701sensitive to bortezomib256
expressivity FYPO_EXT:0000002Cell growth assaysld3ΔNullPECO:0000005, PECO:0000137PMID:21760946

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000311inviable after spore germination with normal, unseptated germ tube morphology237
penetrance FYPO_EXT:0000001Microscopysld3ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1478803478655
2478606476474
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08639 Pfam IPR013948 DNA replication regulator Sld3 211 639 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.18 Da
Charge 38.00
Isoelectric point 9.90
Molecular weight 79.81 kDa
Number of residues 699
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0051321experimental evidencePMID:16303567

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
NDduring GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.4during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.43during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated432
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only620
conserved in fungi4599
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
cdc45DNA replication pre-initiation complex subunit Cdc45 Dosage RescuePMID:12006645
cdc10MBF transcription factor complex subunit Cdc10 Positive GeneticPMID:22681890
prp4serine/threonine protein kinase Prp4 Positive GeneticPMID:22681890
atp11F1-ATPase chaperone Atp11 (predicted) Negative GeneticPMID:22681890
uap2U2 snRNP-associated protein Uap2 Positive GeneticPMID:22681890
psf3GINS complex subunit Psf3 Synthetic Growth DefectPMID:16990792
drc1DNA replication checkpoint protein Drc1 Dosage RescuePMID:21593208
ask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Positive GeneticPMID:22681890
SPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
alp31tubulin specific chaperone cofactor A, Alp31 Negative GeneticPMID:22681890
dad2DASH complex subunit Dad2 Positive GeneticPMID:22681890
sif1Sad1 interacting factor 1 Negative GeneticPMID:22681890
dam1DASH complex subunit Dam1 Positive GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
cdc45DNA replication pre-initiation complex subunit Cdc45 Affinity Capture-WesternPMID:12006645
rad4BRCT domain protein Rad4 Two-hybridPMID:21945095
Two-hybridPMID:21593208
mcm6MCM complex subunit Mcm6 Affinity Capture-WesternPMID:12006645
mcm7MCM complex subunit Mcm7 Affinity Capture-WesternPMID:12006645
mcm2MCM complex subunit Mcm2 Two-hybridPMID:21593208
psf2GINS complex subunit Psf2 Two-hybridPMID:21593208
External References
Database Identifier Description
NBRP SPAC24H6.06 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC24H6.06 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC24H6.06 BioGRID Interaction Datasets
Expression Viewer SPAC24H6.06 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC24H6.06 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC24H6.06 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC24H6.06 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC24H6.06 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC24H6.06 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC24H6.06 Cell Cycle Data
GEO SPAC24H6.06 GEO profiles
PInt SPAC24H6.06 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC24H6.06 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN37/37E10Orfeome Localization Data
UniProtKB/SwissProtQ09761DNA replication regulator sld3
ModBaseQ09761Database of comparative protein structure models
STRINGQ09761Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_592946DNA replication pre-initiation complex subunit Sld3
RefSeq mRNANM_001018347972h- DNA replication pre-initiation complex subunit Sld3 (sld3), mRNA
European Nucleotide ArchiveCAA90850.3ENA Protein Mapping
UniParcUPI00001798DEUniProt Archive

Literature for sld3

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014