sld3 (SPAC24H6.06)


Gene Standard Namesld3 Characterisation Statuspublished
Systematic IDSPAC24H6.06 Feature Typeprotein coding
Synonymsmug175 Name DescriptionMeiotically Upregulated Gene
ProductDNA replication pre-initiation complex subunit Sld3 Product Size699aa, 79.81 kDa
Genomic Location Chromosome I, 478803-476474 (2330nt); CDS:478696-476549 (2148nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
chromatin binding28
Annotation ExtensionEvidenceWith/FromReference
protein binding854
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

DNA replication


Term NameCount
cellular protein localization553
Annotation ExtensionEvidenceWith/FromReference
meiotic cell cycle430
Annotation ExtensionEvidenceWith/FromReference
mitotic DNA replication initiation35
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nuclear replication fork62
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationsld3ΔNull1428
sensitive to bortezomibsld3ΔNull256

Cell Phenotype

Term NameAlleleExpressionCount
inviable after spore germination with normal, unseptated germ tube morphologysld3ΔNull237
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons478803..478655, 478606..476474
Introns478654..478607
mRNA478803..476474
5' UTR478803..478697PMID:21511999
CDS478696..478655, 478606..476549
3' UTR476548..476474PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08639 Pfam IPR013948 DNA replication regulator Sld3 211 639 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.18 Da
Charge 38.00
Isoelectric point 9.90
Molecular weight 79.81 kDa
Number of residues 699
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0051321experimental evidencePMID:16303567

Quantitative Gene Expression

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.4during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.43during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated432
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only620
conserved in fungi4604
conserved in eukaryotes4516
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturescdc45DNA replication pre-initiation complex subunit Cdc45 Affinity Capture-WesternPMID:12006645
affinity captured bycdc45DNA replication pre-initiation complex subunit Cdc45 Affinity Capture-WesternPMID:12006645
binds activation domain construct withmcm2MCM complex subunit Mcm2 Two-hybridPMID:21593208
affinity capturesmcm6MCM complex subunit Mcm6 Affinity Capture-WesternPMID:12006645
affinity capturesmcm7MCM complex subunit Mcm7 Affinity Capture-WesternPMID:12006645
binds activation domain construct withpsf2GINS complex subunit Psf2 Two-hybridPMID:21593208
binds activation domain construct withrad4BRCT domain protein Rad4 Two-hybridPMID:21945095
binds activation domain construct withrad4BRCT domain protein Rad4 Two-hybridPMID:21593208
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withalp31tubulin specific chaperone cofactor A, Alp31 Negative GeneticPMID:22681890
positive genetic interaction withask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
negative genetic interaction withatp11mitochondrial F1-ATPase chaperone Atp11 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withcdc10MBF transcription factor complex subunit Cdc10 Positive GeneticPMID:22681890
rescued by overexpression ofcdc45DNA replication pre-initiation complex subunit Cdc45 Dosage RescuePMID:12006645
overexpression rescuescdc45DNA replication pre-initiation complex subunit Cdc45 Dosage RescuePMID:12006645
positive genetic interaction withdad2DASH complex subunit Dad2 Positive GeneticPMID:22681890
positive genetic interaction withdam1DASH complex subunit Dam1 Positive GeneticPMID:22681890
rescued by overexpression ofdrc1replication preinitiation complex assembly protein Dosage RescuePMID:21593208
positive genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Positive GeneticPMID:22681890
positive genetic interaction withprp4serine/threonine protein kinase Prp4 Positive GeneticPMID:22681890
synthetic growth defect withpsf3GINS complex subunit Psf3 Synthetic Growth DefectPMID:16990792
negative genetic interaction withsif1Sad1 interacting factor 1 Negative GeneticPMID:22681890
positive genetic interaction withSPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
positive genetic interaction withuap2U2 snRNP-associated protein Uap2 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC24H6.06 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC24H6.06 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC24H6.06 BioGRID Interaction Datasets
Expression Viewer SPAC24H6.06 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC24H6.06 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC24H6.06 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC24H6.06 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC24H6.06 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC24H6.06 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC24H6.06 Cell Cycle Data
GEO SPAC24H6.06 GEO profiles
PInt SPAC24H6.06 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC24H6.06 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC24H6.06 Fission yeast phenotypic data & analysis
SPD / RIKEN37/37E10Orfeome Localization Data
UniProtKB/SwissProtQ09761DNA replication regulator sld3
ModBaseQ09761Database of comparative protein structure models
STRINGQ09761Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_592946DNA replication pre-initiation complex subunit Sld3
RefSeq mRNANM_001018347972h- DNA replication pre-initiation complex subunit Sld3 (sld3), mRNA
European Nucleotide ArchiveCAA90850.3ENA Protein Mapping
UniParcUPI00001798DEUniProt Archive

Literature for sld3

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015