SPAC25B8.16


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPAC25B8.16 Feature Typeprotein coding
Synonyms Name Description
ProductRNase P and RNase MRP subunit (predicted) Product Size698aa, 79.93 kDa
Genomic Location Chromosome I, 4187325-4189859 (2535nt); CDS:4187547-4189643 (2097nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000171ribonuclease MRP activity7
contributes_toISOSGD:S000005165GO_REF:0000024
GO:0004526ribonuclease P activity10
contributes_toISOSGD:S000005165GO_REF:0000024
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006379mRNA cleavageISOSGD:S000005165GO_REF:000002420
GO:0006364rRNA processingISOSGD:S000005165GO_REF:0000024182
GO:0001682tRNA 5'-leader removalIEAIPR009723GO_REF:00000023
GO:0008033tRNA processingISOSGD:S000005165GO_REF:000002497
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005655nucleolar ribonuclease P complexISOSGD:S000005165GO_REF:000002410
GO:0005730nucleolusIDAPMID:16823372345
GO:0005634nucleusIDAPMID:107598892730
GO:0000172ribonuclease MRP complexISOSGD:S000005165GO_REF:000002410
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopySPAC25B8.16ΔNullPECO:0000005, PECO:0000137PMID:236978061309
MicroscopySPAC25B8.16ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopySPAC25B8.16ΔNullPECO:0000005, PECO:0000137PMID:23697806564
FYPO:0000314inviable after spore germination with elongated germ tubeMicroscopySPAC25B8.16ΔNullPECO:0000005, PECO:0000137PMID:23697806162
penetrance FYPO_EXT:0000001
FYPO:0002262inviable after spore germination, multiple cell divisions, elongated cellMicroscopySPAC25B8.16ΔNullPECO:0000005, PECO:0000137PMID:2369780687
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
141873254189859

UTRs

Region Start End Reference
three_prime_UTR41896444189859PMID:20118936
five_prime_UTR41873254187546PMID:20118936
three_prime_UTR41896444189859PMID:18641648
five_prime_UTR41873254187546PMID:18641648
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08170 Pfam IPR012590 POPLD 472 568 1
PF06978 Pfam IPR009723 Ribonuclease P/MRP, subunit POP1 43 234 1
PTHR22731 HMMPANTHER 7 645 1
SSF103025 SuperFamily 180 366 3
SSF103025 SuperFamily 456 522 3
Low complexity (SEG) seg 585 597
Low complexity (SEG) seg 115 129

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.51 Da
Charge 41.00
Isoelectric point 10.03
Molecular weight 79.93 kDa
Number of residues 698
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
992.91during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.45during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
1.9during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
External References
Database Identifier Description
NBRP SPAC25B8.16 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC25B8.16 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC25B8.16 BioGRID Interaction Datasets
Expression Viewer SPAC25B8.16 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC25B8.16 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC25B8.16 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC25B8.16 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC25B8.16 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC25B8.16 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC25B8.16 Cell Cycle Data
GEO SPAC25B8.16 GEO profiles
PInt SPAC25B8.16 Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2541548RNase P and RNase MRP subunit (predicted)
EntrezGene2541548RNase P and RNase MRP subunit (predicted)
SPD / RIKEN27/27H04Orfeome Localization Data
UniProtKB/SwissProtQ9UTA4Ribonucleases P/MRP protein subunit pop1
ModBaseQ9UTA4Database of comparative protein structure models
StringQ9UTA4Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594476RNase P and RNase MRP subunit (predicted)
RefSeq mRNANM_001019905972h- RNase P and RNase MRP subunit (predicted) (SPAC25B8.16), mRNA
European Nucleotide ArchiveBAA87135ENA Protein Mapping
European Nucleotide ArchiveCAB61782ENA Protein Mapping
UniParcUPI000006BCD8UniProt Archive

Literature for SPAC25B8.16

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014