tif51 (SPAC26H5.10c)


Gene Standard Nametif51 Characterisation Statusbiological_role_inferred
Systematic IDSPAC26H5.10c Feature Typeprotein coding
Synonyms Name Description
Producttranslation elongation factor eIF5A (predicted) Product Size157aa, 17.15 kDa
Genomic Location Chromosome I, 4142329-4141281 (1049nt); CDS:4142213-4141740 (474nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0003723RNA bindingISOSGD:S000000760GO_REF:0000024628
GO:0043022ribosome bindingISOSGD:S000000760GO_REF:00000249
GO:0003746translation elongation factor activityISOSGD:S000000760GO_REF:000002415
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0002182cytoplasmic translational elongationNASGO_REF:0000051193
GO:0002184cytoplasmic translational terminationNASGO_REF:00000518
GO:0008612peptidyl-lysine modification to peptidyl-hypusineIEAIPR001884GO_REF:00000024
GO:0045901positive regulation of translational elongationISOSGD:S000000760GO_REF:00000242
GO:0045905positive regulation of translational terminationISOSGD:S000000760GO_REF:00000242
GO:0006452translational frameshiftingISOSGD:S000000760GO_REF:00000242
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722316
GO:0022626cytosolic ribosomeISOSGD:S000000760GO_REF:0000024199
GO:0005730nucleolusIDAPMID:16823372355
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopytif51ΔNullPMID:204732893730
Microscopytif51ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopytif51ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
141423294141281
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01287 Pfam IPR020189 Translation elongation factor, IF5A C-terminal 85 152 2
PF00467 Pfam IPR005824 KOW 29 60 9
PS00302 Prosite Patterns IPR019769 Translation elongation factor, IF5A, hypusine site 50 57 2
PTHR11673 HMMPANTHER IPR001884 Translation elongation factor IF5A 3 157 2
2.30.30.30 Gene3D IPR014722 Ribosomal protein L2 domain 2 14 89 13
2.40.50.140 Gene3D IPR012340 Nucleic acid-binding, OB-fold 90 155 48
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 86 155 57
SSF50104 SuperFamily IPR008991 Translation protein SH3-like domain 15 93 16
PIRSF003025 PIRSF IPR001884 Translation elongation factor IF5A 1 156 2
TIGR00037 tigrfam IPR001884 Translation elongation factor IF5A 18 149 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 109.25 Da
Charge -10.00
Isoelectric point 4.51
Molecular weight 17.15 kDa
Number of residues 157
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineIDAS77PMID:182575171669
present during cellular response to thiabendazole
present during cellular response to thiabendazoleIDAS75PMID:18257517
MOD:00696phosphorylated residueNASPMID:182575171921
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
411842during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
387380during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
444415during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
396929during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
295637.05during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
380493during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
61011.12during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
84during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
7.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
cwf2RNA-binding protein Cwf2 Affinity Capture-MSPMID:21386897
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
cid14poly(A) polymerase Cid14 Affinity Capture-MSPMID:21436456
fic1C2 domain protein Fic1 Affinity Capture-MSPMID:19139265
External References
Database Identifier Description
NBRP SPAC26H5.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC26H5.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC26H5.10c BioGRID Interaction Datasets
Expression Viewer SPAC26H5.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC26H5.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC26H5.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC26H5.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC26H5.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC26H5.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC26H5.10c Cell Cycle Data
GEO SPAC26H5.10c GEO profiles
PInt SPAC26H5.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC26H5.10c Peptides identified in tandem mass spectrometry proteomics experiments
WikiGene2542694translation elongation factor eIF5A (predicted)
EntrezGene2542694translation elongation factor eIF5A (predicted)
SPD / RIKEN04/04D03Orfeome Localization Data
UniProtKB/SwissProtP56289Eukaryotic translation initiation factor 5A-1
ModBaseP56289Database of comparative protein structure models
STRINGP56289Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594457translation elongation factor eIF5A (predicted)
RefSeq mRNANM_001019886972h- translation elongation factor eIF5A (predicted) (tif51), mRNA
European Nucleotide ArchiveAB009604ENA EMBL mapping
European Nucleotide ArchiveBAA24001ENA Protein Mapping
European Nucleotide ArchiveCAB16195ENA Protein Mapping
European Nucleotide ArchiveCAB16195.1ENA Protein Mapping
UniParcUPI000012D37FUniProt Archive

Literature for tif51

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014