tif51 (SPAC26H5.10c)


Gene Standard Nametif51 Characterisation Statusbiological_role_inferred
Systematic IDSPAC26H5.10c Feature Typeprotein coding
Synonyms Name Description
Producttranslation elongation factor eIF5A (predicted) Product Size157aa, 17.15 kDa
Genomic Location Chromosome I, 4142329-4141281 (1049nt); CDS:4142213-4141740 (474nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0003723RNA bindingISOSGD:S000000760GO_REF:0000024627
GO:0043022ribosome bindingISOSGD:S000000760GO_REF:00000248
GO:0003746translation elongation factor activityISOSGD:S000000760GO_REF:000002415
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0002182cytoplasmic translational elongationNASGO_REF:0000051193
GO:0002184cytoplasmic translational terminationNASGO_REF:00000518
GO:0008612peptidyl-lysine modification to hypusineIEAIPR001884GO_REF:00000024
GO:0045901positive regulation of translational elongationISOSGD:S000000760GO_REF:00000242
GO:0045905positive regulation of translational terminationISOSGD:S000000760GO_REF:00000242
GO:0006452translational frameshiftingISOSGD:S000000760GO_REF:00000242
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722317
GO:0022626cytosolic ribosomeISOSGD:S000000760GO_REF:0000024198
GO:0005730nucleolusIDAPMID:16823372345
GO:0005634nucleusIDAPMID:168233722730
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopytif51ΔNullPMID:204732893755
Microscopytif51ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopytif51ΔNullPECO:0000005, PECO:0000137PMID:236978063078
penetrance FYPO_EXT:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
141423294141281
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01287 Pfam IPR020189 Translation elongation factor IF5A 85 152 2
PF00467 Pfam IPR005824 KOW 29 60 9
PS00302 Prosite Patterns IPR019769 Translation elongation factor, IF5A, hypusine site 50 57 2
PTHR11673 HMMPANTHER IPR001884 Translation elongation factor IF5A 2 157 2
G3DSA:2.40.50.140 Gene3D IPR012340 90 155 47
G3DSA:2.30.30.30 Gene3D IPR014722 14 89 12
SSF50249 SuperFamily IPR012340 86 155 57
SSF50104 SuperFamily IPR008991 15 93 16
PIRSF003025 PIRSF IPR001884 Translation elongation factor IF5A 1 156 2
TIGR00037 tigrfam IPR001884 Translation elongation factor IF5A 18 149 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 109.25 Da
Charge -10.00
Isoelectric point 4.51
Molecular weight 17.15 kDa
Number of residues 157
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residuePMID:182575171192
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
295637.05during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
61011.12during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
7.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
84during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
cwf2RNA-binding protein Cwf2 Affinity Capture-MSPMID:21386897
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
cid14poly(A) polymerase Cid14 Affinity Capture-MSPMID:21436456
fic1C2 domain protein Fic1 Affinity Capture-MSPMID:19139265
External References
Database Identifier Description
NBRP SPAC26H5.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC26H5.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC26H5.10c BioGRID Interaction Datasets
Expression Viewer SPAC26H5.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC26H5.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC26H5.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC26H5.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC26H5.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC26H5.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC26H5.10c Cell Cycle Data
GEO SPAC26H5.10c GEO profiles
PInt SPAC26H5.10c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2542694translation elongation factor eIF5A (predicted)
EntrezGene2542694translation elongation factor eIF5A (predicted)
SPD / RIKEN04/04D03Orfeome Localization Data
UniProtKB/SwissProtP56289Eukaryotic translation initiation factor 5A-1
ModBaseP56289Database of comparative protein structure models
StringP56289Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594457translation elongation factor eIF5A (predicted)
RefSeq mRNANM_001019886972h- translation elongation factor eIF5A (predicted) (tif51), mRNA
European Nucleotide ArchiveBAA24001ENA Protein Mapping
European Nucleotide ArchiveCAB16195ENA Protein Mapping
UniParcUPI000012D37FUniProt Archive

Literature for tif51

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014