tif51 (SPAC26H5.10c)


Gene Standard Nametif51 Characterisation Statusbiological role inferred
Systematic IDSPAC26H5.10c Feature Typeprotein coding
Synonyms Name Description
Producttranslation elongation factor eIF5A (predicted) Product Size157aa, 17.15 kDa
Genomic Location Chromosome I, 4142329-4141281 (1049nt); CDS:4142213-4141740 (474nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ribosome binding11
Annotation ExtensionEvidenceWith/FromReference
RNA binding588
Annotation ExtensionEvidenceWith/FromReference
translation elongation factor activity16
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic translational elongation194
Annotation ExtensionEvidenceWith/FromReference
translational frameshifting2
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
cytosolic ribosome199
Annotation ExtensionEvidenceWith/FromReference
nucleolus365
Annotation ExtensionEvidenceWith/FromReference
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
increased cell population growth ratetif51Δ256
viable vegetative cell populationtif51Δ3850

Cell Phenotype

Term NameGenotypesCount
increased protein acetylation24
affecting tif51tif51+
viable vegetative cell with normal cell morphologytif51Δ3102
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in hst2 Sirtuin family histone deacetylase Hst2
FYPO affected by mutation in tif51 translation elongation factor eIF5A (predicted)
GO substrate of ubp4 ubiquitin C-terminal hydrolase Ubp4
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4142329..4141281
mRNA4142329..4141281
5' UTR4142329..4142214AU007282
CDS4142213..4141740
3' UTR4141739..4141281AU009021
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01287 Pfam IPR020189 Translation elongation factor, IF5A C-terminal 85 152 2
SM01376 SMART IPR020189 Translation elongation factor, IF5A C-terminal 85 152 2
PS00302 Prosite Patterns IPR019769 Translation elongation factor, IF5A, hypusine site 50 57 2
PTHR11673 HMMPANTHER IPR001884 Translation elongation factor IF5A 2 157 2
2.40.50.140 Gene3D RecQ-mediated genome instability protein 2 90 155 48
2.30.30.30 Gene3D IPR014722 Ribosomal protein L2 domain 2 14 89 13
SSF50104 SuperFamily IPR008991 Translation protein SH3-like domain 15 93 16
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 86 155 57
PIRSF003025 PIRSF IPR001884 Translation elongation factor IF5A 1 156 2
TIGR00037 TIGRFAM IPR001884 Translation elongation factor IF5A 18 149 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 109.25 Da
Charge -10.00
Codon Adaptation Index 0.77
Isoelectric point 4.51
Molecular weight 17.15 kDa
Number of residues 157
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during cellular response to thiabendazoleS75
present during cellular response to thiabendazoleS77
S75, S77
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S75 PMID:18257517
present during cellular response to thiabendazole IDA S77 PMID:18257517
IDA S77 PMID:25720772
IDA S75 PMID:25720772
acetylated residue 14
Annotation ExtensionEvidenceResidueReference
IDA PMID:18292091
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for tif51 (SPAC26H5.10c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
411842during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
387380during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
444415during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
396929during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
380493during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
295637.05during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
61011.12during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
84during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
7.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in archaea242
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC26H5.10c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withSPBC1271.04ceIF-5A-deoxyhypusine synthase (predicted) Two-hybridPMID:26771498
affinity captured bycid14poly(A) polymerase Cid14 Affinity Capture-MSPMID:21436456
affinity captured bycwf2RNA-binding protein Cwf2 Affinity Capture-MSPMID:21386897
affinity captured byfic1C2 domain protein Fic1 Affinity Capture-MSPMID:19139265
External References
Database Identifier Description
NBRP SPAC26H5.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC26H5.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC26H5.10c BioGRID Interaction Datasets
Expression Viewer SPAC26H5.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC26H5.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC26H5.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC26H5.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC26H5.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC26H5.10c Transcriptome Viewer (Bähler Lab)
GEO SPAC26H5.10c GEO profiles
PInt SPAC26H5.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC26H5.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC26H5.10c Fission yeast phenotypic data & analysis
Cyclebase SPAC26H5.10c.1 Cell Cycle Data
SPD / RIKEN04/04D03Orfeome Localization Data
UniProtKB/SwissProtP56289Eukaryotic translation initiation factor 5A-1
ModBaseP56289Database of comparative protein structure models
STRINGP56289Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594457translation elongation factor eIF5A (predicted)
RefSeq mRNANM_001019886972h- translation elongation factor eIF5A (predicted) (tif51), mRNA
European Nucleotide ArchiveAB009604ENA EMBL mapping
European Nucleotide ArchiveCAB16195.1ENA Protein Mapping
UniParcUPI000012D37FUniProt Archive

Literature for tif51

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016