uch1 (SPAC27F1.03c)


Gene Standard Nameuch1 Characterisation Statuspublished
Systematic IDSPAC27F1.03c Feature Typeprotein coding
Synonyms Name Description
Productubiquitin C-terminal hydrolase Uch1 Product Size222aa, 24.91 kDa
Genomic Location Chromosome I, 4321579-4320716 (864nt); CDS:4321475-4320807 (669nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
thiol-dependent ubiquitin-specific protease activity22
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
protein deubiquitination21
Annotation ExtensionEvidenceWith/FromReference
ubiquitin-dependent protein catabolic process189
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4211
Annotation ExtensionEvidenceWith/FromReference
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
normal growth on cisplatinuch1Δ39
viable vegetative cell populationuch1Δ3840

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologyuch1Δ3100
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4321579..4320716
mRNA4321579..4320716
5' UTR4321579..4321476PMID:21511999
CDS4321475..4320807
3' UTR4320806..4320716PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PS00140 Prosite Patterns IPR001578 Peptidase C12, ubiquitin carboxyl-terminal hydrolase 77 93 1
PTHR10589:SF26 HMMPANTHER 2 219 1
PTHR10589 HMMPANTHER IPR001578 Peptidase C12, ubiquitin carboxyl-terminal hydrolase 2 219 2
3.40.532.10 Gene3D IPR001578 Peptidase C12, ubiquitin carboxyl-terminal hydrolase 2 220 2
SSF54001 SuperFamily 1 220 30
PR00707 PRINTS IPR001578 Peptidase C12, ubiquitin carboxyl-terminal hydrolase 77 94 2
PR00707 PRINTS IPR001578 Peptidase C12, ubiquitin carboxyl-terminal hydrolase 153 164 2
PR00707 PRINTS IPR001578 Peptidase C12, ubiquitin carboxyl-terminal hydrolase 4 21 2
PR00707 PRINTS IPR001578 Peptidase C12, ubiquitin carboxyl-terminal hydrolase 173 183 2
PR00707 PRINTS IPR001578 Peptidase C12, ubiquitin carboxyl-terminal hydrolase 39 51 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.22 Da
Charge -13.00
Codon Adaptation Index 0.39
Isoelectric point 4.47
Molecular weight 24.91 kDa
Number of residues 222
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for uch1 (SPAC27F1.03c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
54022during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
60094during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
47941during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
55320during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
11536.93during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
52101during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5420.48during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.7during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2506
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC27F1.03c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
forms complex withned8ubiquitin-like protein modifier Ned8 Reconstituted ComplexPMID:23496905
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC27F1.03c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withset1histone lysine methyltransferase Set1 Positive GeneticPMID:22681890
positive genetic interaction withufd2ubiquitin-protein ligase E4 Ufd2 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withtas3RITS complex subunit 3 Positive GeneticPMID:22681890
positive genetic interaction withatb2tubulin alpha 2 Positive GeneticPMID:22681890
positive genetic interaction withSPAP8A3.07cphospho-2-dehydro-3-deoxyheptonate aldolase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withraf2Rik1-associated factor Raf2 Positive GeneticPMID:22681890
positive genetic interaction withpam17TIM23 translocase complex-associated motor subunit Pam17 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withpsh3ER chaperone SHR3 homologue Psh3 Positive GeneticPMID:22681890
positive genetic interaction withgma12alpha-1,2-galactosyltransferase Gma12 Positive GeneticPMID:22681890
positive genetic interaction withelp5elongator complex subunit Elp5 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withcph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Positive GeneticPMID:22681890
positive genetic interaction withpom1DYRK family protein kinase Pom1 Positive GeneticPMID:18818364
positive genetic interaction withmsh1mitochondrial MutS protein Msh1 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withpex6peroxin-6 (predicted) Positive GeneticPMID:22681890
phenotype enhanced bynep2NEDD8 protease Nep2 Phenotypic EnhancementPMID:23496905
phenotype enhanced bynep1NEDD8 protease Nep1 Phenotypic EnhancementPMID:23496905
phenotype enhanced byuch2ubiquitin C-terminal hydrolase Uch2 Phenotypic EnhancementPMID:23496905
phenotype enhanced bycsn5COP9/signalosome complex subunit Csn5 Phenotypic EnhancementPMID:23496905
enhances phenotype ofuch2ubiquitin C-terminal hydrolase Uch2 Phenotypic EnhancementPMID:23496905
enhances phenotype ofnep1NEDD8 protease Nep1 Phenotypic EnhancementPMID:23496905
enhances phenotype ofnep2NEDD8 protease Nep2 Phenotypic EnhancementPMID:23496905
enhances phenotype ofcsn5COP9/signalosome complex subunit Csn5 Phenotypic EnhancementPMID:23496905
negative genetic interaction withsap145U2 snRNP-associated protein Sap145 Negative GeneticPMID:22681890
negative genetic interaction withclg1cyclin Clg1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmug129Schizosaccharomyces specific protein Mug129 Negative GeneticPMID:22681890
negative genetic interaction withfra2transcriptional repressor protein BolA domain (predicted) Negative GeneticPMID:22681890
negative genetic interaction withryh1GTPase Ryh1 Negative GeneticPMID:22681890
negative genetic interaction withemc1ER membrane protein complex subunit Emc1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC19B12.11czinc finger protein, human ZNF593 ortholog Negative GeneticPMID:18818364
negative genetic interaction withzrg17cation diffusion family zinc transmembrane transporter Zrg17 Negative GeneticPMID:22681890
negative genetic interaction withyip5Rab GTPase binding Yip5 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withlys4homocitrate synthase Negative GeneticPMID:22681890
negative genetic interaction withfhl1forkhead transcription factor Fhl1 Negative GeneticPMID:22681890
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC27F1.03c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC27F1.03c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC27F1.03c BioGRID Interaction Datasets
Expression Viewer SPAC27F1.03c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC27F1.03c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC27F1.03c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC27F1.03c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC27F1.03c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC27F1.03c Transcriptome Viewer (Bähler Lab)
GEO SPAC27F1.03c GEO profiles
PInt SPAC27F1.03c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC27F1.03c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC27F1.03c Fission yeast phenotypic data & analysis
Cyclebase SPAC27F1.03c.1 Cell Cycle Data
SPD / RIKEN42/42H03Orfeome Localization Data
UniProtKB/SwissProtQ10171Probable ubiquitin carboxyl-terminal hydrolase 1
ModBaseQ10171Database of comparative protein structure models
STRINGQ10171Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594531ubiquitin C-terminal hydrolase Uch1
RefSeq mRNANM_001019960972h- ubiquitin C-terminal hydrolase Uch1 (uch1), mRNA

Literature for uch1

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016