ost1 (SPAC27F1.07)


Gene Standard Nameost1 Characterisation Statusbiological role inferred
Systematic IDSPAC27F1.07 Feature Typeprotein coding
Synonyms Name Description
Productdolichyl-diphospho-oligosaccharide-protein glycosyltransferase Ost1 (predicted) Product Size450aa, 51.68 kDa
Genomic Location Chromosome I, 4328945-4330716 (1772nt); CDS:4329104-4330456 (1353nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
protein N-linked glycosylation33
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum596
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane962
Annotation ExtensionEvidenceWith/FromReference
oligosaccharyltransferase complex8
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationost1Δ1451

Cell Phenotype

Term NameGenotypesCount
inviable after spore germination, single or double cell divisionost1Δ107
inviable after spore germination, without cell division, with abnormal germ tube morphologyost1Δ153
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4328945..4330716
mRNA4328945..4330716
5' UTR4328945..4329103PMID:21511999
CDS4329104..4330456
3' UTR4330457..4330716PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF04597 Pfam IPR007676 Ribophorin I 22 447 1
TMhelix TMHMM 426 448 953
PTHR21049:SF0 HMMPANTHER 1 446 1
PTHR21049 HMMPANTHER IPR007676 Ribophorin I 1 446 1
SignalP-noTM signalp 1 18 207

View domain organization at Pfam

Term IDTerm NameReferenceCount
SO:0000418signal_peptide279
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 114.85 Da
Charge -10.50
Codon Adaptation Index 0.49
Isoelectric point 4.86
Molecular weight 51.68 kDa
Number of residues 450
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for ost1 (SPAC27F1.07)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
30577during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
37557during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
31972during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
37692during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
33011during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
16572.33during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
14959.79during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
11during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancercancer_census148
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2506
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC27F1.07 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bypaa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
External References
Database Identifier Description
NBRP SPAC27F1.07 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC27F1.07 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC27F1.07 BioGRID Interaction Datasets
Expression Viewer SPAC27F1.07 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC27F1.07 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC27F1.07 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC27F1.07 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC27F1.07 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC27F1.07 Transcriptome Viewer (Bähler Lab)
GEO SPAC27F1.07 GEO profiles
PInt SPAC27F1.07 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC27F1.07 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC27F1.07 Fission yeast phenotypic data & analysis
Cyclebase SPAC27F1.07.1 Cell Cycle Data
IntEnz2.4.1.119Integrated relational Enzyme database
Rhea2.4.1.119Annotated reactions database
SPD / RIKEN21/21A01Orfeome Localization Data
UniProtKB/SwissProtQ10176Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
ModBaseQ10176Database of comparative protein structure models
STRINGQ10176Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594536dolichyl-diphospho-oligosaccharide-protein glycosyltransferase Ost1 (predicted)
RefSeq mRNANM_001019965972h- dolichyl-diphospho-oligosaccharide-protein glycosyltransferase Ost1 (predicted) (ost1), mRNA
KEGG00510+2.4.99.18N-Glycan biosynthesis
KEGG00513+2.4.99.18Various types of N-glycan biosynthesis
UniPathwayUPA00378with

Literature for ost1

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016