ost1 (SPAC27F1.07)


Gene Standard Nameost1 Characterisation Statusbiological_role_inferred
Systematic IDSPAC27F1.07 Feature Typeprotein coding
Synonyms Name Description
Productdolichyl-diphospho-oligosaccharide-protein glycosyltransferase Ost1 (predicted) Product Size450aa, 51.68 kDa
Genomic Location Chromosome I, 4328945-4330716 (1772nt); CDS:4329104-4330456 (1353nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
protein N-linked glycosylation32
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum595
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane1018
Annotation ExtensionEvidenceWith/FromReference
oligosaccharyltransferase complex8
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationost1ΔNull1427

Cell Phenotype

Term NameAlleleExpressionCount
inviable after spore germination, single or double cell division101
penetrance FYPO_EXT:0000003ost1ΔNull
inviable after spore germination, without cell division, with abnormal germ tube morphology150
penetrance FYPO_EXT:0000001ost1ΔNull
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
143289454330716

UTRs

Region Coordinates Reference
five_prime_UTR4328945..4329103PMID:21511999
three_prime_UTR4330457..4330716PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF04597 Pfam IPR007676 Ribophorin I 21 448 1
TMhelix TMHMM 426 448 959
PTHR21049:SF0 HMMPANTHER 1 446 1
PTHR21049 HMMPANTHER IPR007676 Ribophorin I 1 446 1
SignalP-noTM signalp 1 18 212

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0000418signal_peptide279
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 114.85 Da
Charge -10.50
Isoelectric point 4.86
Molecular weight 51.68 kDa
Number of residues 450
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
30577during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
37557during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
31972during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
37692during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
33011during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
16572.33during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
14959.79during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
11during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancercancer_census142
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
paa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
External References
Database Identifier Description
NBRP SPAC27F1.07 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC27F1.07 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC27F1.07 BioGRID Interaction Datasets
Expression Viewer SPAC27F1.07 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC27F1.07 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC27F1.07 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC27F1.07 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC27F1.07 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC27F1.07 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC27F1.07 Cell Cycle Data
GEO SPAC27F1.07 GEO profiles
PInt SPAC27F1.07 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC27F1.07 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC27F1.07 Fission yeast phenotypic data & analysis
SPD / RIKEN21/21A01Orfeome Localization Data
IntEnz2.4.1.119Integrated relational Enzyme database
Rhea2.4.1.119Annotated reactions database
UniProtKB/SwissProtQ10176Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
ModBaseQ10176Database of comparative protein structure models
STRINGQ10176Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594536dolichyl-diphospho-oligosaccharide-protein glycosyltransferase Ost1 (predicted)
RefSeq mRNANM_001019965972h- dolichyl-diphospho-oligosaccharide-protein glycosyltransferase Ost1 (predicted) (ost1), mRNA
European Nucleotide ArchiveCAA93296.1ENA Protein Mapping
KEGG_Enzyme00510+2.4.99.18N-Glycan biosynthesis
KEGG_Enzyme00513+2.4.99.18Various types of N-glycan biosynthesis
UniParcUPI0000130ED3UniProt Archive
UniPathwayUPA00378Protein modification; protein glycosylation

Literature for ost1

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015