ost1 (SPAC27F1.07)


Gene Standard Nameost1 Characterisation Statusbiological_role_inferred
Systematic IDSPAC27F1.07 Feature Typeprotein coding
Synonyms Name Description
Productdolichyl-diphospho-oligosaccharide-protein glycosyltransferase Ost1 (predicted) Product Size450aa, 51.68 kDa
Genomic Location Chromosome I, 4328945-4330716 (1772nt); CDS:4329104-4330456 (1353nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004579dolichyl-diphosphooligosaccharide-protein glycotransferase activityIEAIPR007676GO_REF:00000028
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006487protein N-linked glycosylationISOSGD:S000003539GO_REF:000002432
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005783endoplasmic reticulumIDAPMID:16823372594
GO:0016021integral component of membraneIEAUniProtKB-KW:KW-0812GO_REF:00000371018
GO:0008250oligosaccharyltransferase complexISOSGD:S000003539GO_REF:00000248
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopyost1ΔNullPMID:204732891338
Microscopyost1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001042inviable after spore germination, single or double cell division97
penetrance FYPO_EXT:0000003Microscopyost1ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0000313inviable after spore germination, without cell division, with abnormal germ tube morphology146
penetrance FYPO_EXT:0000001Microscopyost1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
143289454330716

UTRs

Region Coordinates Reference
five_prime_UTR4328945..4329103PMID:21511999
three_prime_UTR4330457..4330716PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF04597 Pfam IPR007676 Ribophorin I 21 448 1
TMhelix TMHMM 426 448 959
PTHR21049 HMMPANTHER IPR007676 Ribophorin I 1 446 1
PTHR21049:SF0 HMMPANTHER 1 446 1
SignalP-noTM signalp 1 18 212

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0000418signal_peptide279
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 114.85 Da
Charge -10.50
Isoelectric point 4.86
Molecular weight 51.68 kDa
Number of residues 450
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
30577during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
37557during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
31972during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
37692during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
33011during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
16572.33during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
14959.79during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
11during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancercancer_census142
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
paa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
External References
Database Identifier Description
NBRP SPAC27F1.07 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC27F1.07 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC27F1.07 BioGRID Interaction Datasets
Expression Viewer SPAC27F1.07 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC27F1.07 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC27F1.07 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC27F1.07 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC27F1.07 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC27F1.07 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC27F1.07 Cell Cycle Data
GEO SPAC27F1.07 GEO profiles
PInt SPAC27F1.07 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC27F1.07 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC27F1.07 Fission yeast phenotypic data & analysis
SPD / RIKEN21/21A01Orfeome Localization Data
IntEnz2.4.1.119Integrated relational Enzyme database
Rhea2.4.1.119Annotated reactions database
UniProtKB/SwissProtQ10176Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
ModBaseQ10176Database of comparative protein structure models
STRINGQ10176Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594536dolichyl-diphospho-oligosaccharide-protein glycosyltransferase Ost1 (predicted)
RefSeq mRNANM_001019965972h- dolichyl-diphospho-oligosaccharide-protein glycosyltransferase Ost1 (predicted) (ost1), mRNA
European Nucleotide ArchiveCAA93296.1ENA Protein Mapping
UniParcUPI0000130ED3UniProt Archive

Literature for ost1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014