pgt1 (SPAC29B12.10c)


Gene Standard Namepgt1 Characterisation Statuspublished
Systematic IDSPAC29B12.10c Feature Typeprotein coding
Synonymsopt1 Name Description
Productglutathione transmembrane transporter Pgt1 Product Size851aa, 96.30 kDa
Genomic Location Chromosome I, 5434908-5431575 (3334nt); CDS:5434413-5431858 (2556nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
cellular response to cadmium ion11
Annotation ExtensionEvidenceWith/FromReference
glutathione import across plasma membrane1
Annotation ExtensionEvidenceWith/FromReference
glutathione import into cell1
Annotation ExtensionEvidenceWith/FromReference
glutathione transmembrane transport4
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum595
Annotation ExtensionEvidenceWith/FromReference
endoplasmic reticulum membrane205
Annotation ExtensionEvidenceWith/FromReference
integral component of plasma membrane49
Annotation ExtensionEvidenceWith/FromReference
plasma membrane249
Annotation ExtensionEvidenceWith/FromReference
plasma membrane of cell tip12
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationnot recorded (disruption)Not specified1427
normal growth on dipeptide nitrogen sourcepgt1ΔNull3
normal growth on glutathionepgt1+ (wild type)Overexpression1
normal growth on tetrapeptide nitrogen sourcepgt1ΔNull2
sensitive to bortezomib256
expressivity FYPO_EXT:0000003pgt1ΔNull
viable vegetative cell populationnot recorded (disruption)Not specified3781
pgt1ΔNull

Cell Phenotype

Term NameAlleleExpressionCount
decreased cellular glutathione level during vegetative growthnot recorded (disruption)Not specified3
viable vegetative cell with normal cell morphology3092
penetrance FYPO_EXT:0000001pgt1ΔNull
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
154349085431575

UTRs

Region Coordinates Reference
five_prime_UTR5434908..5434414PMID:21511999
three_prime_UTR5431857..5431575PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03169 Pfam IPR004813 Oligopeptide transporter, OPT superfamily 152 811 3
TMhelix TMHMM 712 731 959
TMhelix TMHMM 180 202 959
TMhelix TMHMM 154 176 959
TMhelix TMHMM 596 618 959
TMhelix TMHMM 480 502 959
TMhelix TMHMM 343 365 959
TMhelix TMHMM 263 285 959
TMhelix TMHMM 534 551 959
TMhelix TMHMM 755 777 959
TMhelix TMHMM 790 812 959
TMhelix TMHMM 638 660 959
TMhelix TMHMM 405 427 959
TMhelix TMHMM 561 583 959
PTHR22601 HMMPANTHER 8 851 3
PTHR22601:SF5 HMMPANTHER 8 851 2
Coil ncoils Rabaptin coiled-coil domain 105 133 971
TIGR00728 tigrfam IPR004813 Oligopeptide transporter, OPT superfamily 135 813 3
TIGR00727 tigrfam IPR004648 Tetrapeptide transporter, OPT1/isp4 130 814 3

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.16 Da
Charge 30.00
Isoelectric point 9.65
Molecular weight 96.30 kDa
Number of residues 851
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS12
present during mitotic M phaseS8
S22
present during mitotic M phaseS6
present during mitotic M phaseS22
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S6 PMID:21712547
present during mitotic M phase experimental evidence S8 PMID:21712547
present during mitotic M phase experimental evidence S12 PMID:21712547
experimental evidence S22 PMID:24763107
present during mitotic M phase experimental evidence S22 PMID:21712547
O-phospho-L-threonine 693
present during mitotic M phaseT2
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T2 PMID:21712547
phosphorylated residue 1922
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein leveldecreased during GO:0036346reporter gene assay evidencePMID:18662319
RNA levelincreased in presence of cadmium sulfate expression microarray evidencePMID:12529438

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
716during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
675during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
639during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
812during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3967.21during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
679during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8.1during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by S. cerevisiae HGT1partial PMID:18662319
Catalytic Activity Attributes
DescriptionQualifierReferenceCount
K(m)=63 microM PMID:186623191
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
vps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
ase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:22681890
prp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
php5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
pab2poly(A) binding protein Pab2 Negative GeneticPMID:22681890
SPAC2F3.11exopolyphosphatase (predicted) Negative GeneticPMID:22681890
snf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
xap5xap-5-like protein Negative GeneticPMID:24957674
tor1phosphatidylinositol kinase Tor1 Negative GeneticPMID:22681890
cid12poly(A) polymerase Cid12 Positive GeneticPMID:22681890
swd1Set1C complex subunit Swd1 Negative GeneticPMID:22681890
mpr1histidine-containing response regulator phosphotransferase Mpr1 Negative GeneticPMID:22681890
ask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
alp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
msa1RNA-binding protein Msa1 Negative GeneticPMID:22681890
gsa1glutathione synthetase large and small subunit Gsa1 Synthetic LethalityPMID:19180638
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
atp11mitochondrial F1-ATPase chaperone Atp11 (predicted) Positive GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Positive GeneticPMID:22681890
SPBC1861.07elongin C (predicted) Negative GeneticPMID:22681890
SPCC70.06nuclear export factor (predicted) Negative GeneticPMID:22681890
met14adenylyl-sulfate kinase (predicted) Negative GeneticPMID:22681890
sif1Sad1 interacting factor 1 Negative GeneticPMID:22681890
res1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
tps1alpha,alpha-trehalose-phosphate synthase Negative GeneticPMID:22681890
elp2elongator complex subunit Elp2 (predicted) Positive GeneticPMID:22681890
dot2ESCRT II complex subunit Dot2 Negative GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
knd1Cullin-associated NEDD8-dissociated protein Knd1 (predicted) Negative GeneticPMID:22681890
sdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC29B12.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC29B12.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC29B12.10c BioGRID Interaction Datasets
Expression Viewer SPAC29B12.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC29B12.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC29B12.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC29B12.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC29B12.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC29B12.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC29B12.10c Cell Cycle Data
GEO SPAC29B12.10c GEO profiles
PInt SPAC29B12.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC29B12.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC29B12.10c Fission yeast phenotypic data & analysis
SPD / RIKEN46/46H11Orfeome Localization Data
UniProtKB/SwissProtO14031Glutathione transporter 1
ModBaseO14031Database of comparative protein structure models
STRINGO14031Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594987glutathione transporter Pgt1
RefSeq mRNANM_001020418972h- glutathione transporter Pgt1 (pgt1), mRNA
European Nucleotide ArchiveCAB16254.1ENA Protein Mapping
UniParcUPI000006C320UniProt Archive

Literature for pgt1

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015