pgt1 (SPAC29B12.10c)


Gene Standard Namepgt1 Characterisation Statuspublished
Systematic IDSPAC29B12.10c Feature Typeprotein coding
Synonymsopt1 Name Description
Productglutathione transporter Pgt1 Product Size851aa, 96.30 kDa
Genomic Location Chromosome I, 5434908-5431575 (3334nt); CDS:5434413-5431858 (2556nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0034634glutathione transmembrane transporter activityEXPPMID:191806382
transports glutathioneIDAPMID:18662319
transports glutathione disulfideIDAPMID:18662319
inhibited by CCCPIDAPMID:18662319
inhibited by sodium azideIDAPMID:18662319
transports L-gamma-glutamyl-L-cysteineIMPPMID:18662319
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0071276cellular response to cadmium ionIEPPMID:1252943813
GO:0098709glutathione import across plasma membraneEXPPMID:191806381
GO:0036347glutathione import into cellIDAPMID:186623191
GO:0034775glutathione transmembrane transportIMPPMID:186623194
IDAPMID:18662319
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005783endoplasmic reticulumIDAPMID:16823372594
GO:0005789endoplasmic reticulum membraneIDAPMID:19180638205
GO:0005887integral component of plasma membraneIDAPMID:1866231950
GO:0005886plasma membraneIDAPMID:19180638244
GO:0031520plasma membrane of cell tipIDAPMID:1866231911
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationCell growth assaynot recorded (disruption)Not specifiedPECO:0000103, PECO:0000180PMID:191806381337
Cell growth assaynot recorded (disruption)Not specifiedPECO:0000118, PECO:0000126, PECO:0000180PMID:19180638
FYPO:0001578normal growth on dipeptide nitrogen sourceCell growth assaypgt1ΔNullPMID:222269463
FYPO:0001582normal growth on glutathioneCell growth assaypgt1+ (wild type)OverexpressionPECO:0000102PMID:186623191
FYPO:0001579normal growth on tetrapeptide nitrogen sourceCell growth assaypgt1ΔNullPMID:222269462
FYPO:0001701sensitive to bortezomib256
expressivity FYPO_EXT:0000003Cell growth assaypgt1ΔNullPECO:0000005, PECO:0000137PMID:21760946
FYPO:0002060viable vegetative cell populationMicroscopypgt1ΔNullPECO:0000005, PECO:0000137PMID:236978063751
Cell growth assaynot recorded (disruption)Not specifiedPECO:0000103, PECO:0000126PMID:19180638
Microscopypgt1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001570decreased cellular glutathione levelSubstance quantification evidencenot recorded (disruption)Not specifiedPECO:0000126, PECO:0000103PMID:191806382
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopypgt1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
154349085431575
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03169 Pfam IPR004813 Oligopeptide transporter, OPT superfamily 152 811 3
TMhelix TMHMM 561 583 959
TMhelix TMHMM 534 551 959
TMhelix TMHMM 263 285 959
TMhelix TMHMM 154 176 959
TMhelix TMHMM 638 660 959
TMhelix TMHMM 790 812 959
TMhelix TMHMM 596 618 959
TMhelix TMHMM 712 731 959
TMhelix TMHMM 180 202 959
TMhelix TMHMM 343 365 959
TMhelix TMHMM 480 502 959
TMhelix TMHMM 755 777 959
TMhelix TMHMM 405 427 959
PTHR22601 HMMPANTHER 85 851 3
Coil ncoils Rabaptin coiled-coil domain 105 133 968
TIGR00727 tigrfam IPR004648 Tetrapeptide transporter, OPT1/isp4 130 814 3
TIGR00728 tigrfam IPR004813 Oligopeptide transporter, OPT superfamily 135 813 3

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.16 Da
Charge 30.00
Isoelectric point 9.65
Molecular weight 96.30 kDa
Number of residues 851
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS22PMID:247631071670
present during mitotic M phaseexperimental evidenceS12PMID:21712547
present during mitotic M phaseexperimental evidenceS22PMID:21712547
present during mitotic M phaseexperimental evidenceS8PMID:21712547
present during mitotic M phaseexperimental evidenceS6PMID:21712547
MOD:00047O-phospho-L-threonineexperimental evidenceT2PMID:21712547690
present during mitotic M phase
MOD:00696phosphorylated residueNASPMID:195477441922
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein leveldecreased during GO:0036346reporter gene assay evidencePMID:18662319
RNA levelincreased in presence of cadmium sulfate expression microarray evidencePMID:12529438

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
716during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
675during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
639during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
812during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3967.21during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
679during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8.1during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by S. cerevisiae HGT1partial PMID:18662319
Catalytic Activity Attributes
DescriptionQualifierReferenceCount
K(m)=63 microM PMID:186623191
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4599
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
vps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
ase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:22681890
prp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
php5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
pab2poly(A) binding protein Pab2 Negative GeneticPMID:22681890
SPAC2F3.11exopolyphosphatase (predicted) Negative GeneticPMID:22681890
snf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
xap5xap-5-like protein Negative GeneticPMID:24957674
tor1phosphatidylinositol kinase Tor1 Negative GeneticPMID:22681890
cid12poly(A) polymerase Cid12 Positive GeneticPMID:22681890
swd1Set1C complex subunit Swd1 Negative GeneticPMID:22681890
mpr1histidine-containing response regulator phosphotransferase Mpr1 Negative GeneticPMID:22681890
ask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
alp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
msa1RNA-binding protein Msa1 Negative GeneticPMID:22681890
gsa1glutathione synthetase large subunit Gsa1 Synthetic LethalityPMID:19180638
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
atp11F1-ATPase chaperone Atp11 (predicted) Positive GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Positive GeneticPMID:22681890
SPBC1861.07elongin C (predicted) Negative GeneticPMID:22681890
SPCC70.06nuclear export factor (predicted) Negative GeneticPMID:22681890
met14adenylyl-sulfate kinase (predicted) Negative GeneticPMID:22681890
sif1Sad1 interacting factor 1 Negative GeneticPMID:22681890
res1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
tps1alpha,alpha-trehalose-phosphate synthase Negative GeneticPMID:22681890
elp2elongator complex subunit Elp2 (predicted) Positive GeneticPMID:22681890
dot2ESCRT II complex subunit Dot2 Negative GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
knd1Cullin-associated NEDD8-dissociated protein Knd1 (predicted) Negative GeneticPMID:22681890
sdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC29B12.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC29B12.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC29B12.10c BioGRID Interaction Datasets
Expression Viewer SPAC29B12.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC29B12.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC29B12.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC29B12.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC29B12.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC29B12.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC29B12.10c Cell Cycle Data
GEO SPAC29B12.10c GEO profiles
PInt SPAC29B12.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC29B12.10c Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN46/46H11Orfeome Localization Data
UniProtKB/SwissProtO14031Glutathione transporter 1
ModBaseO14031Database of comparative protein structure models
STRINGO14031Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594987glutathione transporter Pgt1
RefSeq mRNANM_001020418972h- glutathione transporter Pgt1 (pgt1), mRNA
European Nucleotide ArchiveCAB16254.1ENA Protein Mapping
UniParcUPI000006C320UniProt Archive

Literature for pgt1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014