pgt1 (SPAC29B12.10c)


Gene Standard Namepgt1 Characterisation Statuspublished
Systematic IDSPAC29B12.10c Feature Typeprotein coding
Synonymsopt1 Name Description
Productglutathione transmembrane transporter Pgt1 Product Size851aa, 96.30 kDa
Genomic Location Chromosome I, 5434908-5431575 (3334nt); CDS:5434413-5431858 (2556nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
glutathione import across plasma membrane1
Annotation ExtensionEvidenceWith/FromReference
glutathione import into cell1
Annotation ExtensionEvidenceWith/FromReference
glutathione transmembrane transport4
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum596
Annotation ExtensionEvidenceWith/FromReference
endoplasmic reticulum membrane201
Annotation ExtensionEvidenceWith/FromReference
integral component of plasma membrane49
Annotation ExtensionEvidenceWith/FromReference
plasma membrane253
Annotation ExtensionEvidenceWith/FromReference
plasma membrane of cell tip18
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell population (disruption)1452
normal growth on dipeptide nitrogen sourcepgt1Δ3
normal growth on glutathionepgt1+1
normal growth on tetrapeptide nitrogen sourcepgt1Δ2
sensitive to bortezomibpgt1Δ255
viable vegetative cell population (disruption)3844
pgt1Δ

Cell Phenotype

Term NameGenotypesCount
decreased cellular glutathione level during vegetative growth (disruption)3
viable vegetative cell with normal cell morphologypgt1Δ3100
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons5434908..5431575
mRNA5434908..5431575
5' UTR5434908..5434414PMID:21511999
CDS5434413..5431858
3' UTR5431857..5431575PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03169 Pfam IPR004813 Oligopeptide transporter, OPT superfamily 152 811 3
TMhelix TMHMM 480 502 953
TMhelix TMHMM 343 365 953
TMhelix TMHMM 154 176 953
TMhelix TMHMM 712 731 953
TMhelix TMHMM 561 583 953
TMhelix TMHMM 263 285 953
TMhelix TMHMM 790 812 953
TMhelix TMHMM 638 660 953
TMhelix TMHMM 180 202 953
TMhelix TMHMM 534 551 953
TMhelix TMHMM 755 777 953
TMhelix TMHMM 405 427 953
TMhelix TMHMM 596 618 953
PTHR22601:SF16 HMMPANTHER 5 848 2
PTHR22601 HMMPANTHER 5 848 3
Coil ncoils Predicted coiled-coil protein (DUF2205) 105 132 1057
TIGR00728 TIGRFAM IPR004813 Oligopeptide transporter, OPT superfamily 135 813 3
TIGR00727 TIGRFAM IPR004648 Tetrapeptide transporter, OPT1/isp4 130 814 3

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.16 Da
Charge 30.00
Codon Adaptation Index 0.42
Isoelectric point 9.65
Molecular weight 96.30 kDa
Number of residues 851
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1677
present during mitotic M phaseS8
present during mitotic M phaseS6
present during mitotic M phaseS12
present during mitotic M phaseS22
S22
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S8 PMID:21712547
present during mitotic M phase experimental evidence S6 PMID:21712547
present during mitotic M phase experimental evidence S12 PMID:21712547
present during mitotic M phase experimental evidence S22 PMID:21712547
experimental evidence S22 PMID:24763107
O-phospho-L-threonine 701
present during mitotic M phaseT2
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T2 PMID:21712547
phosphorylated residue 1932
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein leveldecreased during GO:0036346reporter gene assay evidencePMID:18662319
RNA levelincreased in presence of cadmium sulfate expression microarray evidencePMID:12529438

Quantitative Gene Expression

View graphical display of gene expression data for pgt1 (SPAC29B12.10c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
716during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
675during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
639during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
812during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
679during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3967.21during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8.1during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by S. cerevisiae HGT1partial PMID:18662319
Catalytic Activity Attributes
DescriptionQualifierReferenceCount
K(m)=63 microM PMID:186623191
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4606
conserved in eukaryotes4516
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC29B12.10c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withgsa1glutathione synthetase large and small subunit Gsa1 Synthetic LethalityPMID:19180638
negative genetic interaction withtps1alpha,alpha-trehalose-phosphate synthase [UDP-forming] Negative GeneticPMID:22681890
negative genetic interaction withSPCC70.06nuclear export factor (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmpr1histidine-containing response regulator phosphotransferase Mpr1 Negative GeneticPMID:22681890
negative genetic interaction withvps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
negative genetic interaction withalp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
negative genetic interaction withphp5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
negative genetic interaction withptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
negative genetic interaction withSPBC1861.07elongin C (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsif1Sad1 interacting factor 1 Negative GeneticPMID:22681890
negative genetic interaction withmsa1RNA-binding protein Msa1 Negative GeneticPMID:22681890
negative genetic interaction withase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:22681890
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
negative genetic interaction withprp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
negative genetic interaction withpab2poly(A) binding protein Pab2 Negative GeneticPMID:22681890
negative genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
negative genetic interaction withSPAC2F3.11exopolyphosphatase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsnf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
negative genetic interaction withdot2ESCRT II complex subunit Dot2 Negative GeneticPMID:22681890
negative genetic interaction withtor1serine/threonine protein kinase Tor1 Negative GeneticPMID:22681890
negative genetic interaction withknd1Cullin-associated NEDD8-dissociated protein Knd1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withxap5xap-5-like protein Negative GeneticPMID:24957674
negative genetic interaction withmet14adenylyl-sulfate kinase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withswd1Set1C complex subunit Swd1 Negative GeneticPMID:22681890
positive genetic interaction withcid12poly(A) polymerase Cid12 Positive GeneticPMID:22681890
positive genetic interaction withsdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Positive GeneticPMID:22681890
positive genetic interaction withask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
positive genetic interaction withelp2elongator complex subunit Elp2 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withatp11mitochondrial F1-ATPase chaperone Atp11 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withhrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC29B12.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC29B12.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC29B12.10c BioGRID Interaction Datasets
Expression Viewer SPAC29B12.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC29B12.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC29B12.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC29B12.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC29B12.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC29B12.10c Transcriptome Viewer (Bähler Lab)
GEO SPAC29B12.10c GEO profiles
PInt SPAC29B12.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC29B12.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC29B12.10c Fission yeast phenotypic data & analysis
Cyclebase SPAC29B12.10c.1 Cell Cycle Data
SPD / RIKEN46/46H11Orfeome Localization Data
UniProtKB/SwissProtO14031Glutathione transporter 1
ModBaseO14031Database of comparative protein structure models
STRINGO14031Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594987glutathione transporter Pgt1
RefSeq mRNANM_001020418972h- glutathione transporter Pgt1 (pgt1), mRNA
European Nucleotide ArchiveCAB16254.1ENA Protein Mapping
UniParcUPI000006C320UniProt Archive

Literature for pgt1

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016