pgt1 (SPAC29B12.10c)

Gene Standard Namepgt1 Characterisation Statuspublished
Systematic IDSPAC29B12.10c Feature Typeprotein coding
Synonymsopt1 Name Description
Productglutathione transporter Pgt1 Product Size851aa, 96.30 kDa
Genomic Location Chromosome I, 5434908-5431575 (3334nt); CDS:5434413-5431858 (2556nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
cellular response to cadmium ion12
Annotation ExtensionEvidenceWith/FromReference
glutathione import across plasma membrane1
Annotation ExtensionEvidenceWith/FromReference
glutathione import into cell1
Annotation ExtensionEvidenceWith/FromReference
glutathione transmembrane transport4
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum594
Annotation ExtensionEvidenceWith/FromReference
endoplasmic reticulum membrane205
Annotation ExtensionEvidenceWith/FromReference
integral component of plasma membrane50
Annotation ExtensionEvidenceWith/FromReference
plasma membrane247
Annotation ExtensionEvidenceWith/FromReference
plasma membrane of cell tip12
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationCell growth assaynot recorded (disruption)Not specifiedPECO:0000118, PECO:0000126, PECO:0000180PMID:191806381338
Cell growth assaynot recorded (disruption)Not specifiedPECO:0000103, PECO:0000180PMID:19180638
FYPO:0001578normal growth on dipeptide nitrogen sourceCell growth assaypgt1ΔNullPMID:222269463
FYPO:0001582normal growth on glutathioneCell growth assaypgt1+ (wild type)OverexpressionPECO:0000102PMID:186623191
FYPO:0001579normal growth on tetrapeptide nitrogen sourceCell growth assaypgt1ΔNullPMID:222269462
FYPO:0001701sensitive to bortezomib256
expressivity FYPO_EXT:0000003Cell growth assaypgt1ΔNullPECO:0000005, PECO:0000137PMID:21760946
FYPO:0002060viable vegetative cell populationCell growth assaynot recorded (disruption)Not specifiedPECO:0000103, PECO:0000126PMID:191806383759
Microscopypgt1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001570decreased cellular glutathione levelSubstance quantification evidencenot recorded (disruption)Not specifiedPECO:0000126, PECO:0000103PMID:191806382
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopypgt1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03169 Pfam IPR004813 Oligopeptide transporter, OPT superfamily 152 811 3
TMhelix TMHMM 405 427 959
TMhelix TMHMM 755 777 959
TMhelix TMHMM 343 365 959
TMhelix TMHMM 480 502 959
TMhelix TMHMM 180 202 959
TMhelix TMHMM 712 731 959
TMhelix TMHMM 596 618 959
TMhelix TMHMM 790 812 959
TMhelix TMHMM 638 660 959
TMhelix TMHMM 263 285 959
TMhelix TMHMM 154 176 959
TMhelix TMHMM 534 551 959
TMhelix TMHMM 561 583 959
PTHR22601 HMMPANTHER 85 851 3
Coil ncoils Rabaptin coiled-coil domain 105 133 968
TIGR00727 tigrfam IPR004648 Tetrapeptide transporter, OPT1/isp4 130 814 3
TIGR00728 tigrfam IPR004813 Oligopeptide transporter, OPT superfamily 135 813 3

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.16 Da
Charge 30.00
Isoelectric point 9.65
Molecular weight 96.30 kDa
Number of residues 851

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS6PMID:217125471670
present during mitotic M phase
present during mitotic M phaseexperimental evidenceS12PMID:21712547
present during mitotic M phaseexperimental evidenceS8PMID:21712547
present during mitotic M phaseexperimental evidenceS22PMID:21712547
experimental evidenceS22PMID:24763107
MOD:00047O-phospho-L-threonineexperimental evidenceT2PMID:21712547692
present during mitotic M phase
MOD:00696phosphorylated residueNASPMID:195477441922
Gene Expression

Qualitative Gene Expression

protein leveldecreased during GO:0036346reporter gene assay evidencePMID:18662319
RNA levelincreased in presence of cadmium sulfate expression microarray evidencePMID:12529438

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
716during GO:0000080PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
675during GO:0000084PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
639during GO:0000085PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
812during GO:0000087PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
3967.21during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
679during GO:0072690PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8.1during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
functionally complemented by S. cerevisiae HGT1partial PMID:18662319
Catalytic Activity Attributes
K(m)=63 microM PMID:186623191
Species Distribution
conserved in fungi4600
conserved in eukaryotes4514

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
vps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
ase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:22681890
prp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
php5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
pab2poly(A) binding protein Pab2 Negative GeneticPMID:22681890
SPAC2F3.11exopolyphosphatase (predicted) Negative GeneticPMID:22681890
snf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
xap5xap-5-like protein Negative GeneticPMID:24957674
tor1phosphatidylinositol kinase Tor1 Negative GeneticPMID:22681890
cid12poly(A) polymerase Cid12 Positive GeneticPMID:22681890
swd1Set1C complex subunit Swd1 Negative GeneticPMID:22681890
mpr1histidine-containing response regulator phosphotransferase Mpr1 Negative GeneticPMID:22681890
ask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
alp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
msa1RNA-binding protein Msa1 Negative GeneticPMID:22681890
gsa1glutathione synthetase large subunit Gsa1 Synthetic LethalityPMID:19180638
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
atp11F1-ATPase chaperone Atp11 (predicted) Positive GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Positive GeneticPMID:22681890
SPBC1861.07elongin C (predicted) Negative GeneticPMID:22681890
SPCC70.06nuclear export factor (predicted) Negative GeneticPMID:22681890
met14adenylyl-sulfate kinase (predicted) Negative GeneticPMID:22681890
sif1Sad1 interacting factor 1 Negative GeneticPMID:22681890
res1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
tps1alpha,alpha-trehalose-phosphate synthase Negative GeneticPMID:22681890
elp2elongator complex subunit Elp2 (predicted) Positive GeneticPMID:22681890
dot2ESCRT II complex subunit Dot2 Negative GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
knd1Cullin-associated NEDD8-dissociated protein Knd1 (predicted) Negative GeneticPMID:22681890
sdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC29B12.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC29B12.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC29B12.10c BioGRID Interaction Datasets
Expression Viewer SPAC29B12.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC29B12.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC29B12.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC29B12.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC29B12.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC29B12.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC29B12.10c Cell Cycle Data
GEO SPAC29B12.10c GEO profiles
PInt SPAC29B12.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC29B12.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC29B12.10c Fission yeast phenotypic data & analysis
SPD / RIKEN46/46H11Orfeome Localization Data
UniProtKB/SwissProtO14031Glutathione transporter 1
ModBaseO14031Database of comparative protein structure models
STRINGO14031Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594987glutathione transporter Pgt1
RefSeq mRNANM_001020418972h- glutathione transporter Pgt1 (pgt1), mRNA
European Nucleotide ArchiveCAB16254.1ENA Protein Mapping
UniParcUPI000006C320UniProt Archive

Literature for pgt1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014