SPAC29B12.14c


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPAC29B12.14c Feature Typeprotein coding
Synonyms Name Description
Productpurine transporter (predicted) Product Size590aa, 65.23 kDa
Genomic Location Chromosome I, 5442552-5439647 (2906nt); CDS:5441621-5439849 (1773nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
uracil:cation symporter activity2
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cation transmembrane transport128
Annotation ExtensionEvidenceWith/FromReference
nucleobase-containing compound transport80
Annotation ExtensionEvidenceWith/FromReference
nucleotide transport18
Annotation ExtensionEvidenceWith/FromReference
pyrimidine-containing compound transmembrane transport10
Annotation ExtensionEvidenceWith/FromReference
uracil transport2
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2315
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane1018
Annotation ExtensionEvidenceWith/FromReference
nucleus2740
Annotation ExtensionEvidenceWith/FromReference
plasma membrane247
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000088sensitive to hydroxyureaCell growth assaySPAC29B12.14cΔNullPECO:0000137, PECO:0000005PMID:23173672514
FYPO:0000089sensitive to methyl methanesulfonateCell growth assaySPAC29B12.14cΔNullPECO:0000137, PECO:0000005PMID:23173672219
FYPO:0000091sensitive to thiabendazoleCell growth assaySPAC29B12.14cΔNullPECO:0000137, PECO:0000005PMID:23173672176
FYPO:0002060viable vegetative cell populationMicroscopySPAC29B12.14cΔNullPECO:0000005, PECO:0000137PMID:236978063759
MicroscopySPAC29B12.14cΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001MicroscopySPAC29B12.14cΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
154425525439647

UTRs

Region Coordinates Reference
five_prime_UTR5442552..5441622PMID:21511999
three_prime_UTR5439848..5439647PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02133 Pfam IPR001248 Permease, cytosine/purines, uracil, thiamine, allantoin 55 506 3
TMhelix TMHMM 466 488 959
TMhelix TMHMM 135 157 959
TMhelix TMHMM 294 316 959
TMhelix TMHMM 223 245 959
TMhelix TMHMM 257 279 959
TMhelix TMHMM 417 439 959
TMhelix TMHMM 351 373 959
TMhelix TMHMM 194 213 959
TMhelix TMHMM 93 115 959
TMhelix TMHMM 388 410 959
TMhelix TMHMM 501 523 959
PTHR30618 HMMPANTHER 1 582 3
PTHR30618:SF0 HMMPANTHER 1 582 3
TIGR00800 tigrfam IPR012681 Nucleobase cation symporter-1, NCS1 50 491 2

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 110.56 Da
Charge 8.00
Isoelectric point 8.09
Molecular weight 65.23 kDa
Number of residues 590
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS567PMID:217125471670
present during mitotic M phase
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0034599expression microarray evidencePMID:12529438

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1606.16during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.3during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.84during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999432
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4600
conserved in eukaryotes4514
conserved in bacteria1000
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
xap5xap-5-like protein Positive GeneticPMID:24957674
External References
Database Identifier Description
NBRP SPAC29B12.14c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC29B12.14c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC29B12.14c BioGRID Interaction Datasets
Expression Viewer SPAC29B12.14c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC29B12.14c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC29B12.14c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC29B12.14c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC29B12.14c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC29B12.14c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC29B12.14c Cell Cycle Data
GEO SPAC29B12.14c GEO profiles
PInt SPAC29B12.14c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC29B12.14c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC29B12.14c Fission yeast phenotypic data & analysis
SPD / RIKEN45/45G04Orfeome Localization Data
UniProtKB/SwissProtO14035Uncharacterized permease C29B12.14c
ModBaseO14035Database of comparative protein structure models
STRINGO14035Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594991purine transporter (predicted)
RefSeq mRNANM_001020422972h- purine transporter (predicted) (SPAC29B12.14c), mRNA
European Nucleotide ArchiveCAB16258.2ENA Protein Mapping
UniParcUPI000228F45AUniProt Archive

Literature for SPAC29B12.14c

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014