Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPAC2C4.12c Feature Typeprotein coding
Synonyms Name Description
ProducttRNA 2'-phosphotransferase Tpt1 (predicted) Product Size365aa, 41.03 kDa
Genomic Location Chromosome I, 4281428-4279661 (1768nt); CDS:4280970-4279873 (1098nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000215tRNA 2'-phosphotransferase activityISSUniProtKB:Q12272GO_REF:00000011
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006388tRNA splicing, via endonucleolytic cleavage and ligationISSUniProtKB:Q12272GO_REF:00000016
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopySPAC2C4.12cΔNullPMID:204732891309
FYPO:0002060viable vegetative cell populationMicroscopySPAC2C4.12cΔNullPECO:0000005, PECO:0000137PMID:236978063755

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001511inviable vegetative cell, abnormal cell shape, normal cell sizeMicroscopySPAC2C4.12cΔNullPECO:0000137, PECO:0000005PMID:23697806198
expressivity FYPO_EXT:0000003
penetrance FYPO_EXT:0000001
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Start End Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF09811 Pfam IPR019191 Essential protein Yae1, N-terminal 280 317 2
PF01885 Pfam IPR002745 Phosphotransferase KptA/Tpt1 36 213 1
PTHR32107 HMMPANTHER 1 259 1
PTHR32107:SF0 HMMPANTHER 1 259 1
SSF56399 SuperFamily 40 213 1
Coil ncoils Rabaptin coiled-coil domain 324 345 975
Low complexity (SEG) seg 17 31

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.41 Da
Charge 7.00
Isoelectric point 8.02
Molecular weight 41.03 kDa
Number of residues 365

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residuePMID:182575171192
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
4963.28during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
3107.85during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1.2during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
0.24during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
gene structure updated PMID:21511999426
possible gene merge, unusual domain combination1
Species Distribution
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3394

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
External References
Database Identifier Description
NBRP SPAC2C4.12c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC2C4.12c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC2C4.12c BioGRID Interaction Datasets
Expression Viewer SPAC2C4.12c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC2C4.12c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC2C4.12c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC2C4.12c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC2C4.12c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC2C4.12c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC2C4.12c Cell Cycle Data
GEO SPAC2C4.12c GEO profiles
PInt SPAC2C4.12c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2541572tRNA 2'-phosphotransferase Tpt1 (predicted)
EntrezGene2541572tRNA 2'-phosphotransferase Tpt1 (predicted)
SPD / RIKEN43/43G05Orfeome Localization Data
UniProtKB/SwissProtO14045Putative tRNA 2'-phosphotransferase
ModBaseO14045Database of comparative protein structure models
StringO14045Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594515tRNA 2'-phosphotransferase Tpt1 (predicted)
RefSeq mRNANM_001019944972h- tRNA 2'-phosphotransferase Tpt1 (predicted) (SPAC2C4.12c), mRNA
European Nucleotide ArchiveCAB16372ENA Protein Mapping
UniParcUPI000228F4C8UniProt Archive

Literature for SPAC2C4.12c

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014