tpt1 (SPAC2C4.12c)


Gene Standard Nametpt1 Characterisation Statusbiological role inferred
Systematic IDSPAC2C4.12c Feature Typeprotein coding
Synonyms Name Description
ProducttRNA 2'-phosphotransferase Tpt1 (predicted) Product Size365aa, 41.03 kDa
Genomic Location Chromosome I, 4281428-4279661 (1768nt); CDS:4280970-4279873 (1098nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
tRNA 2'-phosphotransferase activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationtpt1Δ1455

Cell Phenotype

Term NameGenotypesCount
inviable vegetative cell, abnormal cell shape, normal cell sizetpt1Δ199
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4281428..4279661
mRNA4281428..4279661
5' UTR4281428..4280971PMID:21511999
CDS4280970..4279873
3' UTR4279872..4279661PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF09811 Pfam IPR019191 Essential protein Yae1, N-terminal 280 317 2
PF01885 Pfam IPR002745 Phosphotransferase KptA/Tpt1 39 210 1
PTHR12684 HMMPANTHER 31 248 1
PTHR12684:SF2 HMMPANTHER 31 248 1
SSF56399 SuperFamily 40 213 1
Coil ncoils Predicted coiled-coil protein (DUF2205) 324 344 1055

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.41 Da
Charge 7.00
Codon Adaptation Index 0.36
Isoelectric point 8.02
Molecular weight 41.03 kDa
Number of residues 365
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during cellular response to thiabendazoleS237
S179, S237
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S237 PMID:18257517
IDA S179 PMID:25720772
IDA S237 PMID:25720772
O-phospho-L-threonineT181 1085
Annotation ExtensionEvidenceResidueReference
IDA T181 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for tpt1 (SPAC2C4.12c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
6354during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5202during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
6422during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
6464during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
6527during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4963.28during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
3107.85during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.2during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.24during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999435
possible gene merge, unusual domain combination1
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3093
conserved in fungi4607
conserved in eukaryotes4517
conserved in bacteria1005
conserved in metazoa3427
conserved in vertebrates3402
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC2C4.12c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withlto1ribosome biogenesis protein Lto1 (predicted) Two-hybridPMID:26771498
External References
Database Identifier Description
NBRP SPAC2C4.12c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC2C4.12c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC2C4.12c BioGRID Interaction Datasets
Expression Viewer SPAC2C4.12c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC2C4.12c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC2C4.12c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC2C4.12c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC2C4.12c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC2C4.12c Transcriptome Viewer (Bähler Lab)
GEO SPAC2C4.12c GEO profiles
PInt SPAC2C4.12c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC2C4.12c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC2C4.12c Fission yeast phenotypic data & analysis
Cyclebase SPAC2C4.12c.1 Cell Cycle Data
SPD / RIKEN43/43G05Orfeome Localization Data
UniProtKB/SwissProtO14045Putative tRNA 2'-phosphotransferase
ModBaseO14045Database of comparative protein structure models
STRINGO14045Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594515tRNA 2'-phosphotransferase Tpt1 (predicted)
RefSeq mRNANM_001019944972h- tRNA 2'-phosphotransferase Tpt1 (predicted) (SPAC2C4.12c), mRNA
European Nucleotide ArchiveCAB16372.2ENA Protein Mapping
UniParcUPI000228F4C8UniProt Archive

Literature for tpt1

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016