SPAC2C4.12c


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPAC2C4.12c Feature Typeprotein coding
Synonyms Name Description
ProducttRNA 2'-phosphotransferase Tpt1 (predicted) Product Size365aa, 41.03 kDa
Genomic Location Chromosome I, 4281428-4279661 (1768nt); CDS:4280970-4279873 (1098nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000215tRNA 2'-phosphotransferase activityISSUniProtKB:Q12272GO_REF:00000241
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006388tRNA splicing, via endonucleolytic cleavage and ligationISSUniProtKB:Q12272GO_REF:00000246
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005634nucleusISSUniProtKB:Q12272GO_REF:00000242740
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopySPAC2C4.12cΔNullPMID:204732891338
MicroscopySPAC2C4.12cΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001511inviable vegetative cell, abnormal cell shape, normal cell size199
expressivity FYPO_EXT:0000003
penetrance FYPO_EXT:0000001
MicroscopySPAC2C4.12cΔNullPECO:0000137, PECO:0000005PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
142814284279661

UTRs

Region Coordinates Reference
five_prime_UTR4281428..4280971PMID:21511999
three_prime_UTR4279872..4279661PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF09811 Pfam IPR019191 Essential protein Yae1, N-terminal 280 317 2
PF01885 Pfam IPR002745 Phosphotransferase KptA/Tpt1 36 213 1
PTHR32107 HMMPANTHER 1 259 1
PTHR32107:SF0 HMMPANTHER 1 259 1
SSF56399 SuperFamily 40 213 1
Coil ncoils Rabaptin coiled-coil domain 324 345 968

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.41 Da
Charge 7.00
Isoelectric point 8.02
Molecular weight 41.03 kDa
Number of residues 365
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineIDAS237PMID:182575171670
present during cellular response to thiabendazole
MOD:00696phosphorylated residueNASPMID:182575171922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
6354during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
5202during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
6422during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
6464during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
6527during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
4963.28during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
3107.85during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.2during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.24during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999432
possible gene merge, unusual domain combination1
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3425
conserved in vertebrates3400
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


External References
Database Identifier Description
NBRP SPAC2C4.12c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC2C4.12c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC2C4.12c BioGRID Interaction Datasets
Expression Viewer SPAC2C4.12c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC2C4.12c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC2C4.12c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC2C4.12c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC2C4.12c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC2C4.12c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC2C4.12c Cell Cycle Data
GEO SPAC2C4.12c GEO profiles
PInt SPAC2C4.12c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC2C4.12c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC2C4.12c Fission yeast phenotypic data & analysis
SPD / RIKEN43/43G05Orfeome Localization Data
UniProtKB/SwissProtO14045Putative tRNA 2'-phosphotransferase
ModBaseO14045Database of comparative protein structure models
STRINGO14045Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594515tRNA 2'-phosphotransferase Tpt1 (predicted)
RefSeq mRNANM_001019944972h- tRNA 2'-phosphotransferase Tpt1 (predicted) (SPAC2C4.12c), mRNA
European Nucleotide ArchiveCAB16372.2ENA Protein Mapping
UniParcUPI000228F4C8UniProt Archive

Literature for SPAC2C4.12c

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014