tpt1 (SPAC2C4.12c)


Gene Standard Nametpt1 Characterisation Statusbiological_role_inferred
Systematic IDSPAC2C4.12c Feature Typeprotein coding
Synonyms Name Description
ProducttRNA 2'-phosphotransferase Tpt1 (predicted) Product Size365aa, 41.03 kDa
Genomic Location Chromosome I, 4281428-4279661 (1768nt); CDS:4280970-4279873 (1098nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
tRNA 2'-phosphotransferase activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationtpt1ΔNull1428

Cell Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell, abnormal cell shape, normal cell sizetpt1ΔNull199
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4281428..4279661
mRNA4281428..4279661
5' UTR4281428..4280971PMID:21511999
CDS4280970..4279873
3' UTR4279872..4279661PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01885 Pfam IPR002745 Phosphotransferase KptA/Tpt1 36 213 1
PF09811 Pfam IPR019191 Essential protein Yae1, N-terminal 280 317 2
PTHR12684:SF2 HMMPANTHER 1 224 1
PTHR12684 HMMPANTHER 1 224 1
SSF56399 SuperFamily 40 213 1
Coil ncoils Rabaptin coiled-coil domain 324 345 968

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.41 Da
Charge 7.00
Isoelectric point 8.02
Molecular weight 41.03 kDa
Number of residues 365
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during cellular response to thiabendazoleS237
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S237 PMID:18257517
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
6354during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5202during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
6422during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
6464during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
4963.28during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
6527during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3107.85during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.2during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.24during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999432
possible gene merge, unusual domain combination1
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in bacteria1000
conserved in metazoa3430
conserved in vertebrates3405
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


External References
Database Identifier Description
NBRP SPAC2C4.12c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC2C4.12c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC2C4.12c BioGRID Interaction Datasets
Expression Viewer SPAC2C4.12c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC2C4.12c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC2C4.12c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC2C4.12c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC2C4.12c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC2C4.12c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC2C4.12c Cell Cycle Data
GEO SPAC2C4.12c GEO profiles
PInt SPAC2C4.12c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC2C4.12c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC2C4.12c Fission yeast phenotypic data & analysis
SPD / RIKEN43/43G05Orfeome Localization Data
UniProtKB/SwissProtO14045Putative tRNA 2'-phosphotransferase
ModBaseO14045Database of comparative protein structure models
STRINGO14045Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594515tRNA 2'-phosphotransferase Tpt1 (predicted)
RefSeq mRNANM_001019944972h- tRNA 2'-phosphotransferase Tpt1 (predicted) (SPAC2C4.12c), mRNA
European Nucleotide ArchiveCAB16372.2ENA Protein Mapping
UniParcUPI000228F4C8UniProt Archive

Literature for tpt1

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015