SPAC2E1P5.03


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPAC2E1P5.03 Feature Typeprotein coding
Synonyms Name Description
ProductDNAJ domain protein Erj5 (predicted) Product Size303aa, 34.79 kDa
Genomic Location Chromosome I, 3326830-3329028 (2199nt); CDS:3326898-3327809 (912nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030544Hsp70 protein bindingISMPFAM:PF00226GO_REF:000000126
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006457protein foldingNASGO_REF:0000051111
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005783endoplasmic reticulumIDAPMID:16823372594
GO:0016021integral component of membraneIEAUniProtKB-KW:KW-0812GO_REF:00000371018
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopySPAC2E1P5.03ΔNullPMID:204732893751
MicroscopySPAC2E1P5.03ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001MicroscopySPAC2E1P5.03ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
133268303329028
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00226 Pfam IPR001623 DnaJ domain 40 99 24
SM00271 SMART IPR001623 DnaJ domain 39 94 23
TMhelix TMHMM 122 144 959
TMhelix TMHMM 5 27 959
PS50076 Prosite Profiles IPR001623 DnaJ domain 40 102 24
PTHR24078 HMMPANTHER 14 136 6
1.10.287.110 Gene3D IPR001623 DnaJ domain 36 117 26
SSF46565 SuperFamily IPR001623 DnaJ domain 37 123 25
SignalP-noTM signalp 1 16 212
PR00625 PRINTS IPR001623 DnaJ domain 60 75 21
PR00625 PRINTS IPR001623 DnaJ domain 42 60 21
PR00625 PRINTS IPR001623 DnaJ domain 94 113 21

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.81 Da
Charge 25.50
Isoelectric point 10.59
Molecular weight 34.79 kDa
Number of residues 303
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS273PMID:217125471670
present during mitotic M phase
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1659during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1352during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2552.09during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1852during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1809.88during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.2during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.52during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
SPCC584.11cSvf1 family protein Svf1 Negative GeneticPMID:22681890
res1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
ddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:22681890
SPBC13E7.03cRNA hairpin binding protein (predicted) Positive GeneticPMID:22681890
sfp1transcription factor Sfp1 (predicted) Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPAC2E1P5.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC2E1P5.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC2E1P5.03 BioGRID Interaction Datasets
Expression Viewer SPAC2E1P5.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC2E1P5.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC2E1P5.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC2E1P5.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC2E1P5.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC2E1P5.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC2E1P5.03 Cell Cycle Data
GEO SPAC2E1P5.03 GEO profiles
PInt SPAC2E1P5.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC2E1P5.03 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN12/12F04Orfeome Localization Data
UniProtKB/SwissProtQ9P7C1Uncharacterized J domain-containing protein C2E1P5.03
ModBaseQ9P7C1Database of comparative protein structure models
STRINGQ9P7C1Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594141DNAJ domain protein Erj5 (predicted)
RefSeq PeptideNP_594142geranylgeranyltransferase I beta subunit Cwg2
RefSeq mRNANM_001019565972h- DNAJ domain protein Erj5 (predicted) (SPAC2E1P5.03), mRNA
RefSeq mRNANM_001019566972h- geranylgeranyltransferase I beta subunit Cwg2 (cwg2), mRNA
European Nucleotide ArchiveCAB86346.1ENA Protein Mapping
UniParcUPI0000069DFBUniProt Archive

Literature for SPAC2E1P5.03

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014