Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPAC2E1P5.03 Feature Typeprotein coding
Synonyms Name Description
ProductDNAJ domain protein Erj5 (predicted) Product Size303aa, 34.79 kDa
Genomic Location Chromosome I, 3326830-3329028 (2199nt); CDS:3326898-3327809 (912nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
Hsp70 protein binding26
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

protein folding

Term NameCount
protein folding106
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum594
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane1018
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopySPAC2E1P5.03ΔNullPECO:0000005, PECO:0000137PMID:236978063759

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001MicroscopySPAC2E1P5.03ΔNullPECO:0000005, PECO:0000137PMID:23697806
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00226 Pfam IPR001623 DnaJ domain 40 99 24
SM00271 SMART IPR001623 DnaJ domain 39 94 23
TMhelix TMHMM 122 144 959
TMhelix TMHMM 5 27 959
PS50076 Prosite Profiles IPR001623 DnaJ domain 40 102 24
PTHR24078 HMMPANTHER 14 136 6 Gene3D IPR001623 DnaJ domain 36 117 26
SSF46565 SuperFamily IPR001623 DnaJ domain 37 123 25
SignalP-noTM signalp 1 16 212
PR00625 PRINTS IPR001623 DnaJ domain 60 75 21
PR00625 PRINTS IPR001623 DnaJ domain 42 60 21
PR00625 PRINTS IPR001623 DnaJ domain 94 113 21

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.81 Da
Charge 25.50
Isoelectric point 10.59
Molecular weight 34.79 kDa
Number of residues 303

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS273PMID:217125471670
present during mitotic M phase
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1659during GO:0000080PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
1352during GO:0000085PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
2552.09during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
1852during GO:0072690PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
1809.88during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.2during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
0.52during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
SPCC584.11cSvf1 family protein Svf1 Negative GeneticPMID:22681890
res1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
ddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:22681890
sgf73SAGA complex subunit Sgf73 Negative GeneticPMID:25076038
SPBC13E7.03cRNA hairpin binding protein (predicted) Positive GeneticPMID:22681890
sfp1transcription factor Sfp1 (predicted) Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC2E1P5.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC2E1P5.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC2E1P5.03 BioGRID Interaction Datasets
Expression Viewer SPAC2E1P5.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC2E1P5.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC2E1P5.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC2E1P5.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC2E1P5.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC2E1P5.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC2E1P5.03 Cell Cycle Data
GEO SPAC2E1P5.03 GEO profiles
PInt SPAC2E1P5.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC2E1P5.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC2E1P5.03 Fission yeast phenotypic data & analysis
SPD / RIKEN12/12F04Orfeome Localization Data
UniProtKB/SwissProtQ9P7C1Uncharacterized J domain-containing protein C2E1P5.03
ModBaseQ9P7C1Database of comparative protein structure models
STRINGQ9P7C1Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594141DNAJ domain protein Erj5 (predicted)
RefSeq PeptideNP_594142geranylgeranyltransferase I beta subunit Cwg2
RefSeq mRNANM_001019565972h- DNAJ domain protein Erj5 (predicted) (SPAC2E1P5.03), mRNA
RefSeq mRNANM_001019566972h- geranylgeranyltransferase I beta subunit Cwg2 (cwg2), mRNA
European Nucleotide ArchiveCAB86346.1ENA Protein Mapping
UniParcUPI0000069DFBUniProt Archive

Literature for SPAC2E1P5.03

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014