erj5 (SPAC2E1P5.03)


Gene Standard Nameerj5 Characterisation Statusbiological_role_inferred
Systematic IDSPAC2E1P5.03 Feature Typeprotein coding
Synonyms Name Description
ProductDNAJ domain protein Erj5 (predicted) Product Size303aa, 34.79 kDa
Genomic Location Chromosome I, 3326830-3329028 (2199nt); CDS:3326898-3327809 (912nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
Hsp70 protein binding26
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

protein folding


Term NameCount
protein folding104
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum596
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane1018
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
increased cell population growth rateerj5ΔNull246
viable vegetative cell populationerj5ΔNull3792

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologyerj5ΔNull3092
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3326830..3329028
mRNA3326830..3329028
5' UTR3326830..3326897PMID:21511999
CDS3326898..3327809
3' UTR3327810..3329028PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00226 Pfam IPR001623 DnaJ domain 40 99 24
SM00271 SMART IPR001623 DnaJ domain 39 94 23
TMhelix TMHMM 5 27 959
TMhelix TMHMM 122 144 959
PS50076 Prosite Profiles IPR001623 DnaJ domain 40 102 24
PTHR24078:SF122 HMMPANTHER 1 187 1
PTHR24078 HMMPANTHER 1 187 7
1.10.287.110 Gene3D IPR001623 DnaJ domain 36 117 26
SSF46565 SuperFamily IPR001623 DnaJ domain 37 123 25
SignalP-noTM signalp 1 16 212
PR00625 PRINTS IPR001623 DnaJ domain 94 113 21
PR00625 PRINTS IPR001623 DnaJ domain 60 75 21
PR00625 PRINTS IPR001623 DnaJ domain 42 60 21

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.81 Da
Charge 25.50
Isoelectric point 10.59
Molecular weight 34.79 kDa
Number of residues 303
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS273
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S273 PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1659during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1352during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1852during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2552.09during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1809.88during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.2during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.52during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byhhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
affinity captured byhhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
negative genetic interaction withhrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:22681890
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
negative genetic interaction withsfp1transcription factor Sfp1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsgf73SAGA complex subunit Sgf73 Negative GeneticPMID:25076038
positive genetic interaction withSPBC13E7.03cRNA hairpin binding protein (predicted) Positive GeneticPMID:22681890
negative genetic interaction withSPCC584.11cSvf1 family protein Svf1 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC2E1P5.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC2E1P5.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC2E1P5.03 BioGRID Interaction Datasets
Expression Viewer SPAC2E1P5.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC2E1P5.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC2E1P5.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC2E1P5.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC2E1P5.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC2E1P5.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC2E1P5.03 Cell Cycle Data
GEO SPAC2E1P5.03 GEO profiles
PInt SPAC2E1P5.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC2E1P5.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC2E1P5.03 Fission yeast phenotypic data & analysis
SPD / RIKEN12/12F04Orfeome Localization Data
UniProtKB/SwissProtQ9P7C1Uncharacterized J domain-containing protein C2E1P5.03
ModBaseQ9P7C1Database of comparative protein structure models
STRINGQ9P7C1Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594141DNAJ domain protein Erj5 (predicted)
RefSeq PeptideNP_594142geranylgeranyltransferase I beta subunit Cwg2
RefSeq mRNANM_001019565972h- DNAJ domain protein Erj5 (predicted) (SPAC2E1P5.03), mRNA
RefSeq mRNANM_001019566972h- geranylgeranyltransferase I beta subunit Cwg2 (cwg2), mRNA
European Nucleotide ArchiveCAB86346.1ENA Protein Mapping
UniParcUPI0000069DFBUniProt Archive

Literature for erj5

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015