SPAC2F3.16


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPAC2F3.16 Feature Typeprotein coding
Synonyms Name Description
Productubiquitin-protein ligase E3 (predicted) Product Size425aa, 49.90 kDa
Genomic Location Chromosome I, 3957085-3959427 (2343nt); CDS:3957143-3958606 (1464nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004842ubiquitin-protein ligase activityISMPFAM:PF00097GO_REF:000000183
GO:0008270zinc ion bindingIEAIPR001841GO_REF:0000002248
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0016567protein ubiquitinationISMPFAM:PF00097GO_REF:0000001115
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopySPAC2F3.16ΔNullPECO:0000005, PECO:0000137PMID:236978063755
MicroscopySPAC2F3.16ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopySPAC2F3.16ΔNullPECO:0000005, PECO:0000137PMID:236978063078
penetrance FYPO_EXT:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
139570853957442
239575123957844
339579003958004
439580673959427

UTRs

Region Start End Reference
three_prime_UTR39586073959427PMID:21511999
five_prime_UTR39570853957142PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF14599 Pfam 317 375 1
PF05495 Pfam IPR008913 Zinc finger, CHY-type 142 216 4
PF13639 Pfam IPR001841 Zinc finger, RING-type 270 312 14
SM00184 SMART IPR001841 Zinc finger, RING-type 271 312 51
PS51270 Prosite Profiles IPR017921 Zinc finger, CTCHY-type 204 270 1
PS50089 Prosite Profiles IPR001841 Zinc finger, RING-type 271 312 44
PS51266 Prosite Profiles IPR008913 Zinc finger, CHY-type 135 202 4
PTHR21319:SF2 HMMPANTHER 4 374 1
PTHR21319 HMMPANTHER 4 374 2
G3DSA:3.30.40.10 Gene3D IPR013083 267 312 84
G3DSA:2.20.28.10 Gene3D IPR004039 342 382 3
SSF161219 SuperFamily 135 203 4
SSF57850 SuperFamily 269 320 59
SSF161245 SuperFamily 205 264 1
Low complexity (SEG) seg 9 20
Low complexity (SEG) seg 54 64

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000137zinc finger proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000137
PBO:0000153zf-C2H2 typeTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000153
PBO:0000173zf-C3HC4 type (RING finger)Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000173
PBO:0000176ubiquitin-protein ligase E3Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000176
PBO:0002306zf-CHYTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0002306
PBO:0002307LIM domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0002307

Protein Properties

Ave. residue weight 117.42 Da
Charge -4.50
Isoelectric point 6.10
Molecular weight 49.90 kDa
Number of residues 425
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
567.39during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
2.9during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog579
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
def1RNAPII degradation factor Def1 (predicted) Positive GeneticPMID:22681890
pmc2mediator complex subunit Pmc2/Med1 Negative GeneticPMID:22681890
liz1pantothenate transporter Liz1 Negative GeneticPMID:22681890
hip4histone promoter control protein Hip4 Negative GeneticPMID:18818364
etf1electron transfer flavoprotein alpha subunit EtfA (predicted) Negative GeneticPMID:22681890
atf21transcription factor, Atf-CREB family Atf21 Negative GeneticPMID:22681890
vht1vitamin H transporter Vht1 Negative GeneticPMID:22681890
erp2COPII-coated vesicle component Erp2/3/4 (predicted) Negative GeneticPMID:22681890
atd1aldehyde dehydrogenase (predicted) Negative GeneticPMID:22681890
cho1phosphatidyl-N-methylethanolamine N-methyltransferase (predicted) Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
External References
Database Identifier Description
NBRP SPAC2F3.16 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC2F3.16 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC2F3.16 BioGRID Interaction Datasets
Expression Viewer SPAC2F3.16 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC2F3.16 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC2F3.16 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC2F3.16 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC2F3.16 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC2F3.16 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC2F3.16 Cell Cycle Data
GEO SPAC2F3.16 GEO profiles
PInt SPAC2F3.16 Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2541608ubiquitin-protein ligase E3 (predicted)
EntrezGene2541608ubiquitin-protein ligase E3 (predicted)
SPD / RIKEN44/44G06Orfeome Localization Data
UniProtKB/SwissProtO14099Uncharacterized RING finger protein C2F3.16
ModBaseO14099Database of comparative protein structure models
StringO14099Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594394ubiquitin-protein ligase E3 (predicted)
RefSeq mRNANM_001019817972h- ubiquitin-protein ligase E3 (predicted) (SPAC2F3.16), mRNA
European Nucleotide ArchiveCAB16270ENA Protein Mapping
UniParcUPI000006A5DAUniProt Archive

Literature for SPAC2F3.16

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014