SPAC2F3.16


Gene Standard NameUnassigned Characterisation Statusbiological role inferred
Systematic IDSPAC2F3.16 Feature Typeprotein coding
Synonyms Name Description
Productubiquitin-protein ligase E3 (predicted) Product Size425aa, 49.90 kDa
Genomic Location Chromosome I, 3957085-3959427 (2343nt); CDS:3957143-3958606 (1464nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ubiquitin protein ligase activity65
Annotation ExtensionEvidenceWith/FromReference
zinc ion binding238
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
protein ubiquitination118
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationSPAC2F3.16Δ3837

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologySPAC2F3.16Δ3100
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3957085..3957442, 3957512..3957844, 3957900..3958004, 3958067..3959427
Intron3957443..3957511, 3957845..3957899, 3958005..3958066
5' UTR3957085..3957142PMID:21511999
3' UTR3958607..3959427PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF14599 Pfam 318 375 1
PF05495 Pfam IPR008913 Zinc finger, CHY-type 142 215 4
SM00184 SMART IPR001841 Zinc finger, RING-type 271 312 30
PS50089 Prosite Profiles IPR001841 Zinc finger, RING-type 271 312 44
PS51270 Prosite Profiles IPR017921 Zinc finger, CTCHY-type 204 270 1
PS51266 Prosite Profiles IPR008913 Zinc finger, CHY-type 135 202 4
PTHR21319 HMMPANTHER 77 375 2
3.30.40.10 Gene3D IPR013083 Zinc finger, RING/FYVE/PHD-type 267 312 86
2.20.28.10 Gene3D IPR004039 Rubredoxin-type fold 342 382 3
SSF57850 SuperFamily 269 320 59
0053829 SuperFamily 205 264 1
0053828 SuperFamily 135 203 4

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000137zinc finger proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000137
PBO:0000153zf-C2H2 typeTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000153
PBO:0000173zf-C3HC4 type (RING finger)Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000173
PBO:0000176ubiquitin-protein ligase E3Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000176
PBO:0002306zf-CHYTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0002306
PBO:0002307LIM domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0002307

Protein Properties

Ave. residue weight 117.42 Da
Charge -4.50
Codon Adaptation Index 0.47
Isoelectric point 6.10
Molecular weight 49.90 kDa
Number of residues 425
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for SPAC2F3.16

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3811during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3989during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4250during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
567.39during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.9during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog601
predominantly single copy (one to one)3092
conserved in fungi4605
conserved in eukaryotes4515
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC2F3.16 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC2F3.16 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withliz1pantothenate transmembrane transporter Liz1 Negative GeneticPMID:22681890
negative genetic interaction withcho1phosphatidyl-N-dimethylethanolamine N-methyltransferase Negative GeneticPMID:22681890
negative genetic interaction witherp2COPII-coated vesicle component Erp2/3/4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpmc2mediator complex subunit Med1 Negative GeneticPMID:22681890
negative genetic interaction withatf21transcription factor, Atf-CREB family Atf21 Negative GeneticPMID:22681890
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:18818364
negative genetic interaction withetf1electron transfer flavoprotein alpha subunit EtfA (predicted) Negative GeneticPMID:22681890
negative genetic interaction withatd1aldehyde dehydrogenase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withvht1vitamin H transmembrane transporter Vht1 Negative GeneticPMID:22681890
positive genetic interaction withdef1RNAPII degradation factor Def1 (predicted) Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC2F3.16 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC2F3.16 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC2F3.16 BioGRID Interaction Datasets
Expression Viewer SPAC2F3.16 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC2F3.16 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC2F3.16 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC2F3.16 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC2F3.16 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC2F3.16 Transcriptome Viewer (Bähler Lab)
GEO SPAC2F3.16 GEO profiles
PInt SPAC2F3.16 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC2F3.16 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC2F3.16 Fission yeast phenotypic data & analysis
Cyclebase SPAC2F3.16.1 Cell Cycle Data
SPD / RIKEN44/44G06Orfeome Localization Data
UniProtKB/SwissProtO14099Uncharacterized RING finger protein C2F3.16
ModBaseO14099Database of comparative protein structure models
STRINGO14099Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594394ubiquitin-protein ligase E3 (predicted)
RefSeq mRNANM_001019817972h- ubiquitin-protein ligase E3 (predicted) (SPAC2F3.16), mRNA

Literature for SPAC2F3.16

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016