SPAC2F3.16


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPAC2F3.16 Feature Typeprotein coding
Synonyms Name Description
Productubiquitin-protein ligase E3 (predicted) Product Size425aa, 49.90 kDa
Genomic Location Chromosome I, 3957085-3959427 (2343nt); CDS:3957143-3958606 (1464nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ubiquitin protein ligase activity67
Annotation ExtensionEvidenceWith/FromReference
zinc ion binding248
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
protein ubiquitination117
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
viable vegetative cell populationSPAC2F3.16ΔNull3783

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologySPAC2F3.16ΔNull3092
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
139570853957442
239575123957844
339579003958004
439580673959427

UTRs

Region Coordinates Reference
five_prime_UTR3957085..3957142PMID:21511999
three_prime_UTR3958607..3959427PMID:21511999
mRNA3957085..3959427
exon3957143..3957442,3957512..3957844,3957900..3958004,3958067..3958606
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF14599 Pfam 317 375 1
PF05495 Pfam IPR008913 Zinc finger, CHY-type 142 216 4
PF13639 Pfam IPR001841 Zinc finger, RING-type 270 312 15
SM00184 SMART IPR001841 Zinc finger, RING-type 271 312 30
PS51266 Prosite Profiles IPR008913 Zinc finger, CHY-type 135 202 4
PS51270 Prosite Profiles IPR017921 Zinc finger, CTCHY-type 204 270 1
PS50089 Prosite Profiles IPR001841 Zinc finger, RING-type 271 312 44
PTHR21319 HMMPANTHER 4 415 2
PTHR21319:SF2 HMMPANTHER 4 415 1
3.30.40.10 Gene3D IPR013083 Zinc finger, RING/FYVE/PHD-type 267 312 86
2.20.28.10 Gene3D IPR004039 Rubredoxin-type fold 342 382 3
0053828 SuperFamily 135 203 4
0053829 SuperFamily 205 264 1
SSF57850 SuperFamily 269 320 59

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000137zinc finger proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000137
PBO:0000153zf-C2H2 typeTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000153
PBO:0000173zf-C3HC4 type (RING finger)Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000173
PBO:0000176ubiquitin-protein ligase E3Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000176
PBO:0002306zf-CHYTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0002306
PBO:0002307LIM domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0002307

Protein Properties

Ave. residue weight 117.42 Da
Charge -4.50
Isoelectric point 6.10
Molecular weight 49.90 kDa
Number of residues 425
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3811during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3989during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
4250during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
567.39during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.9during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog597
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withatd1aldehyde dehydrogenase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withatf21transcription factor, Atf-CREB family Atf21 Negative GeneticPMID:22681890
negative genetic interaction withcho1phosphatidyl-N-methylethanolamine N-methyltransferase (predicted) Negative GeneticPMID:22681890
positive genetic interaction withdef1RNAPII degradation factor Def1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction witherp2COPII-coated vesicle component Erp2/3/4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withetf1electron transfer flavoprotein alpha subunit EtfA (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:18818364
negative genetic interaction withliz1pantothenate transmembrane transporter Liz1 Negative GeneticPMID:22681890
negative genetic interaction withpmc2mediator complex subunit Pmc2/Med1 Negative GeneticPMID:22681890
negative genetic interaction withvht1vitamin H transmembrane transporter Vht1 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC2F3.16 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC2F3.16 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC2F3.16 BioGRID Interaction Datasets
Expression Viewer SPAC2F3.16 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC2F3.16 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC2F3.16 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC2F3.16 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC2F3.16 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC2F3.16 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC2F3.16 Cell Cycle Data
GEO SPAC2F3.16 GEO profiles
PInt SPAC2F3.16 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC2F3.16 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC2F3.16 Fission yeast phenotypic data & analysis
SPD / RIKEN44/44G06Orfeome Localization Data
UniProtKB/SwissProtO14099Uncharacterized RING finger protein C2F3.16
ModBaseO14099Database of comparative protein structure models
STRINGO14099Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594394ubiquitin-protein ligase E3 (predicted)
RefSeq mRNANM_001019817972h- ubiquitin-protein ligase E3 (predicted) (SPAC2F3.16), mRNA
European Nucleotide ArchiveCAB16270.1ENA Protein Mapping
UniParcUPI000006A5DAUniProt Archive

Literature for SPAC2F3.16

Search: Europe PMC or PubMed

Release Version: PomBase:25_49 - 02 Feb 2015