ptc3 (SPAC2G11.07c)


Gene Standard Nameptc3 Characterisation Statuspublished
Systematic IDSPAC2G11.07c Feature Typeprotein coding
Synonyms Name DescriptionProtein Phosphatase 2C homolog
Productprotein phosphatase 2c homolog 3 Product Size414aa, 44.86 kDa
Genomic Location Chromosome I, 821920-819768 (2153nt); CDS:821704-820163 (1542nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
metal ion binding743
Annotation ExtensionEvidenceWith/FromReference
protein serine/threonine phosphatase activity15
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

signaling


Term NameCount
inactivation of MAPK activity involved in osmosensory signaling pathway3
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
viable vegetative cell populationptc3ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
small vegetative cellptc3ΔNull7
viable vegetative cell with normal cell morphologyptc3ΔNull3094
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons821920..821377, 821118..821045, 821005..819768
Introns821376..821119, 821044..821006L34882
mRNA821920..819768
5' UTR821920..821705PMID:21511999
CDS821704..821377, 821118..821045, 821005..820163
3' UTR820162..819768PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00481 Pfam IPR001932 Protein phosphatase 2C (PP2C)-like domain 24 273 6
SM00332 SMART IPR001932 Protein phosphatase 2C (PP2C)-like domain 13 286 7
PS01032 Prosite Patterns IPR000222 Protein phosphatase 2C, manganese/magnesium aspartate binding site 57 65 4
PTHR13832 HMMPANTHER IPR015655 Protein phosphatase 2C 1 327 5
3.60.40.10 Gene3D IPR001932 Protein phosphatase 2C (PP2C)-like domain 11 296 7
SSF81606 SuperFamily IPR001932 Protein phosphatase 2C (PP2C)-like domain 3 288 7

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.35 Da
Charge -21.50
Isoelectric point 4.49
Molecular weight 44.86 kDa
Number of residues 414
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS389
present during mitotic M phaseS390
present during mitotic M phaseS391
S389
Annotation ExtensionEvidenceResidueReference
experimental evidence S389 PMID:24763107
present during mitotic M phase experimental evidence S389 PMID:21712547
present during mitotic M phase experimental evidence S390 PMID:21712547
present during mitotic M phase experimental evidence S391 PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
43406during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
43460during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
41820during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
43823during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
45537during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
27346.25during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
28701.24during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
13during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
4.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bypaa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withalp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
negative genetic interaction withamk2AMP-activated protein kinase beta subunit Amk2 Negative GeneticPMID:22681890
negative genetic interaction witharp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
positive genetic interaction witharp6actin-like protein Arp6 Positive GeneticPMID:18818364
negative genetic interaction withase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:18818364
negative genetic interaction withase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:22681890
positive genetic interaction withatb2tubulin alpha 2 Positive GeneticPMID:22681890
negative genetic interaction withatp11mitochondrial F1-ATPase chaperone Atp11 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withcdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
positive genetic interaction withcxr1mRNA processing factor Positive GeneticPMID:22681890
negative genetic interaction withddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:18818364
negative genetic interaction withemc1ER membrane protein complex subunit Emc1 (predicted) Negative GeneticPMID:22681890
positive genetic interaction witherg5C-22 sterol desaturase Erg5 Positive GeneticPMID:22681890
negative genetic interaction withfar10SIP/FAR complex FHA domain subunit Far10/Csc1 Negative GeneticPMID:22681890
negative genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
negative genetic interaction withgcn20AAA family ATPase Gcn20 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withgcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
positive genetic interaction withhus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
negative genetic interaction withiqw1WD repeat protein, Iqw1 Negative GeneticPMID:22681890
positive genetic interaction withliz1pantothenate transmembrane transporter Liz1 Positive GeneticPMID:22681890
negative genetic interaction withmga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Negative GeneticPMID:18818364
negative genetic interaction withmga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmid1medial ring protein Mid1 Negative GeneticPMID:22681890
negative genetic interaction withmpr1histidine-containing response regulator phosphotransferase Mpr1 Negative GeneticPMID:22681890
negative genetic interaction withmug113T5orf172 family protein Negative GeneticPMID:22681890
negative genetic interaction withpar1protein phosphatase regulatory subunit Par1 Negative GeneticPMID:22681890
overexpression rescuespck2protein kinase C (PKC)-like Pck2 Dosage RescuePMID:17881729
positive genetic interaction withphp5CCAAT-binding factor complex subunit Php5 Positive GeneticPMID:22681890
positive genetic interaction withpkp1mitochondrial pyruvate dehydrogenase (lipoamide) kinase Pkp1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withpmp1dual-specificity MAP kinase phosphatase Pmp1 Negative GeneticPMID:22681890
overexpression rescuesppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Dosage RescuePMID:17881729
negative genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
synthetic growth defect withptc1protein phosphatase 2C Ptc1 Synthetic Growth DefectPMID:7859738
enhances phenotype ofptc1protein phosphatase 2C Ptc1 Phenotypic EnhancementPMID:17881729
synthetic growth defect withptc2protein phosphatase 2C Ptc2 Synthetic Growth DefectPMID:7859738
synthetic growth defect withpyp1tyrosine phosphatase Pyp1 Synthetic Growth DefectPMID:9211944
negative genetic interaction withpyp1tyrosine phosphatase Pyp1 Negative GeneticPMID:22681890
positive genetic interaction withraf2Rik1-associated factor Raf2 Positive GeneticPMID:18818364
negative genetic interaction withreb1RNA polymerase I transcription termination factor/ RNA polymerase II transcription factor Reb1 Negative GeneticPMID:22681890
negative genetic interaction withrpl430260S ribosomal protein L37a (predicted) Negative GeneticPMID:22681890
positive genetic interaction withsck2serine/threonine protein kinase Sck2 Positive GeneticPMID:24463365
positive genetic interaction withser2phosphoserine phosphatase Ser2 (predicted) Positive GeneticPMID:18818364
negative genetic interaction withset1histone lysine methyltransferase Set1 Negative GeneticPMID:22681890
negative genetic interaction withsfp1transcription factor Sfp1 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withski2Ski complex RNA helicase Ski2 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withSPBC16H5.13WD repeat protein, human WDR7 ortholog Positive GeneticPMID:22681890
positive genetic interaction withSPBC1861.07elongin C (predicted) Positive GeneticPMID:18818364
negative genetic interaction withSPBPB2B2.18Schizosaccharomyces specific protein Negative GeneticPMID:22681890
negative genetic interaction withSPCP1E11.10ankyrin repeat protein, unknown biological role Negative GeneticPMID:22681890
positive genetic interaction withsut1alpha-glucoside transmembrane transporter Sut1 Positive GeneticPMID:22681890
positive genetic interaction withswc5Swr1 complex subunit Swc5 Positive GeneticPMID:18818364
positive genetic interaction withvph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Positive GeneticPMID:18818364
negative genetic interaction withvps3CORVET complex subunit, GTPase regulator Vps3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withvps5retromer complex subunit Vps5 Negative GeneticPMID:22681890
synthetically rescued bywis1MAP kinase kinase Wis1 Synthetic RescuePMID:7859738
negative genetic interaction withymr1phosphatidylinositol-3-phosphatase, myotubularin family (predicted) Negative GeneticPMID:18818364
negative genetic interaction withypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC2G11.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC2G11.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC2G11.07c BioGRID Interaction Datasets
Expression Viewer SPAC2G11.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC2G11.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC2G11.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC2G11.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC2G11.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC2G11.07c Transcriptome Viewer (Bähler Lab)
GEO SPAC2G11.07c GEO profiles
PInt SPAC2G11.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC2G11.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC2G11.07c Fission yeast phenotypic data & analysis
Cyclebase SPAC2G11.07c.1 Cell Cycle Data
IntEnz3.1.3.16Integrated relational Enzyme database
Rhea3.1.3.16Annotated reactions database
SPD / RIKEN25/25A02Orfeome Localization Data
UniProtKB/SwissProtQ09173Protein phosphatase 2C homolog 3
ModBaseQ09173Database of comparative protein structure models
STRINGQ09173Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593087protein phosphatase 2C
RefSeq mRNANM_001018485972h- protein phosphatase 2C (ptc3), mRNA
European Nucleotide ArchiveAAA67321.1ENA Protein Mapping
European Nucleotide ArchiveCAA91172.1ENA Protein Mapping
UniParcUPI0000130FE1UniProt Archive

Literature for ptc3

Search: Europe PMC or PubMed

Release Version: PomBase:26_52 - 11 May 2015