ptc3 (SPAC2G11.07c)


Gene Standard Nameptc3 Characterisation Statuspublished
Systematic IDSPAC2G11.07c Feature Typeprotein coding
Synonyms Name DescriptionProtein Phosphatase 2C homolog
Productprotein phosphatase 2c homolog 3 Product Size414aa, 44.86 kDa
Genomic Location Chromosome I, 821920-819768 (2153nt); CDS:821704-820163 (1542nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0046872metal ion bindingIEAUniProtKB-KW:KW-0479GO_REF:0000037747
GO:0004721phosphoprotein phosphatase activityIEAUniProtKB-KW:KW-0904GO_REF:000003738
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000173inactivation of MAPK activity involved in osmosensory signaling pathwayIMPPMID:78597383
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722315
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopyptc3ΔNullPMID:204732893759
Microscopyptc3ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000023small cellMicroscopyptc3ΔNullPMID:785973872
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopyptc3ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1821920821377
2821118821045
3821005819768

UTRs

Region Coordinates Reference
five_prime_UTR821920..821705PMID:21511999
three_prime_UTR820162..819768PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00481 Pfam IPR001932 Protein phosphatase 2C (PP2C)-like domain 24 273 6
SM00332 SMART IPR001932 Protein phosphatase 2C (PP2C)-like domain 13 286 7
PS01032 Prosite Patterns IPR000222 Protein phosphatase 2C, manganese/magnesium aspartate binding site 57 65 4
PTHR13832:SF103 HMMPANTHER 1 323 2
PTHR13832 HMMPANTHER IPR015655 Protein phosphatase 2C 1 323 5
3.60.40.10 Gene3D IPR001932 Protein phosphatase 2C (PP2C)-like domain 11 296 7
SSF81606 SuperFamily IPR001932 Protein phosphatase 2C (PP2C)-like domain 3 288 7

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.35 Da
Charge -21.50
Isoelectric point 4.49
Molecular weight 44.86 kDa
Number of residues 414
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS390PMID:217125471670
present during mitotic M phase
experimental evidenceS389PMID:24763107
present during mitotic M phaseexperimental evidenceS389PMID:21712547
present during mitotic M phaseexperimental evidenceS391PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
43406during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
43460during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
41820during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
43823during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
27346.25during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
45537during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
28701.24during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
13during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
4.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
wis1MAP kinase kinase Wis1 Synthetic RescuePMID:7859738
cdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
ase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
pck2protein kinase C (PKC)-like Pck2 Dosage RescuePMID:17881729
set1histone lysine methyltransferase Set1 Negative GeneticPMID:22681890
pmp1dual-specificity MAP kinase phosphatase Pmp1 Negative GeneticPMID:22681890
reb1RNA polymerase I transcription termination factor/ RNA polymerase II transcription factor Reb1 Negative GeneticPMID:22681890
rpl430260S ribosomal protein L37a (predicted) Negative GeneticPMID:22681890
vps3CORVET complex subunit, GTPase regulator Vps3 (predicted) Negative GeneticPMID:22681890
pyp1tyrosine phosphatase Pyp1 Synthetic Growth DefectPMID:9211944
Negative GeneticPMID:22681890
mga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
SPCC550.03cSki complex RNA helicase Ski2 (predicted) Positive GeneticPMID:18818364
mid1medial ring protein Mid1 Negative GeneticPMID:22681890
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:22681890
SPBC16H5.13WD repeat protein, human WDR7 ortholog Positive GeneticPMID:22681890
ddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:18818364
raf2Rik1-associated factor Raf2 Positive GeneticPMID:18818364
mpr1histidine-containing response regulator phosphotransferase Mpr1 Negative GeneticPMID:22681890
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Positive GeneticPMID:18818364
hus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
far10SIP/FAR complex FHA domain subunit Far10/Csc1 Negative GeneticPMID:22681890
erg5C-22 sterol desaturase Erg5 Positive GeneticPMID:22681890
alp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
SPBC29A3.09cAAA family ATPase Gcn20 (predicted) Negative GeneticPMID:22681890
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
amk2AMP-activated protein kinase beta subunit Amk2 Negative GeneticPMID:22681890
par1protein phosphatase regulatory subunit Par1 Negative GeneticPMID:22681890
ptc1protein phosphatase 2C Ptc1 Synthetic Growth DefectPMID:7859738
Phenotypic EnhancementPMID:17881729
atp11F1-ATPase chaperone Atp11 (predicted) Negative GeneticPMID:18818364
swc5Swr1 complex subunit Swc5 Positive GeneticPMID:18818364
iqw1WD repeat protein, Iqw1 Negative GeneticPMID:22681890
SPBPB2B2.18Schizosaccharomyces specific protein Negative GeneticPMID:22681890
arp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
ymr1phosphatidylinositol-3-phosphatase, myotubularin family (predicted) Negative GeneticPMID:18818364
php5CCAAT-binding factor complex subunit Php5 Positive GeneticPMID:22681890
pkp1mitochondrial pyruvate dehydrogenase (lipoamide) kinase Pkp1 (predicted) Positive GeneticPMID:22681890
ptc2protein phosphatase 2C Ptc2 Synthetic Growth DefectPMID:7859738
sut1alpha-glucoside transporter Sut1 Positive GeneticPMID:22681890
sck2serine/threonine protein kinase Sck2 Positive GeneticPMID:24463365
SPBC1861.07elongin C (predicted) Positive GeneticPMID:18818364
atb2tubulin alpha 2 Positive GeneticPMID:22681890
sfp1transcription factor Sfp1 (predicted) Negative GeneticPMID:22681890
ser2phosphoserine phosphatase Ser2 (predicted) Positive GeneticPMID:18818364
cxr1mRNA processing factor Positive GeneticPMID:22681890
emc1ER membrane protein complex subunit Emc1 (predicted) Negative GeneticPMID:22681890
ppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Dosage RescuePMID:17881729
SPCP1E11.10ankyrin repeat protein, unknown biological role Negative GeneticPMID:22681890
arp6actin-like protein Arp6 Positive GeneticPMID:18818364
prw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
vps5retromer complex subunit Vps5 Negative GeneticPMID:22681890
mug113T5orf172 family protein Negative GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
liz1pantothenate transporter Liz1 Positive GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
paa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
External References
Database Identifier Description
NBRP SPAC2G11.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC2G11.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC2G11.07c BioGRID Interaction Datasets
Expression Viewer SPAC2G11.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC2G11.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC2G11.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC2G11.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC2G11.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC2G11.07c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC2G11.07c Cell Cycle Data
GEO SPAC2G11.07c GEO profiles
PInt SPAC2G11.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC2G11.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC2G11.07c Fission yeast phenotypic data & analysis
IntEnz3.1.3.16Integrated relational Enzyme database
Rhea3.1.3.16Annotated reactions database
SPD / RIKEN25/25A02Orfeome Localization Data
UniProtKB/SwissProtQ09173Protein phosphatase 2C homolog 3
ModBaseQ09173Database of comparative protein structure models
STRINGQ09173Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593087protein phosphatase 2C
RefSeq mRNANM_001018485972h- protein phosphatase 2C (ptc3), mRNA
European Nucleotide ArchiveAAA67321.1ENA Protein Mapping
European Nucleotide ArchiveCAA91172.1ENA Protein Mapping
UniParcUPI0000130FE1UniProt Archive

Literature for ptc3

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014