ptc3 (SPAC2G11.07c)


Gene Standard Nameptc3 Characterisation Statuspublished
Systematic IDSPAC2G11.07c Feature Typeprotein coding
Synonyms Name DescriptionProtein Phosphatase 2C homolog
Productprotein phosphatase 2c homolog 3 Product Size414aa, 44.86 kDa
Genomic Location Chromosome I, 821920-819768 (2153nt); CDS:821704-820163 (1542nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
MAP kinase threonine phosphatase activity2
Annotation ExtensionEvidenceWith/FromReference
metal ion binding751
Annotation ExtensionEvidenceWith/FromReference
protein serine/threonine phosphatase activity16
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

signaling


GO Cellular Component
Term NameCount
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationptc3Δ3840

Cell Phenotype

Term NameGenotypesCount
decreased protein threonine phosphorylation during cellular response to heat2
affecting sty1ptc3+
normal protein threonine phosphorylation during cellular response to heat3
affecting sty1ptc3Δ
small vegetative cellptc3Δ16
viable vegetative cell with normal cell morphologyptc3Δ3100

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
sensitive to torin1ptc2Δ, ptc1Δ, ptc3Δ
viable vegetative cell populationptc1Δ, ptc2Δ, ptc3Δ

Cell Phenotype

Term NameGenotypes
abnormal mitotic cell cycle regulation upon nitrogen source shiftptc1Δ, ptc2Δ, ptc3Δ
decreased protein phosphorylation during cellular response to heat
affecting sty1ptc1Δ, ptc3Δ
elongated cell during nitrogen starvationptc2Δ, ptc1Δ, ptc3Δ
increased protein phosphorylation during cellular response to hydrogen peroxide
affecting sty1ptc1Δ, ptc2Δ, ptc3Δ
increased protein phosphorylation during vegetative growth
affecting ssp2ptc1Δ, ptc2Δ, ptc3Δ
Target Of
OntologyRelationshipGeneProduct
GO substrate of sst2 human AMSH/STAMBP protein homolog, ubiquitin specific-protease
GO substrate of ubp15 ubiquitin C-terminal hydrolase Ubp15
GO substrate of ubp4 ubiquitin C-terminal hydrolase Ubp4
GO substrate of ubp5 ubiquitin C-terminal hydrolase Ubp5
GO substrate of ubp9 ubiquitin C-terminal hydrolase Ubp9
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons821920..821377, 821118..821045, 821005..819768
Intron821376..821119, 821044..821006
mRNA821920..819768
5' UTR821920..821705PMID:21511999
CDS821704..821377, 821118..821045, 821005..820163
3' UTR820162..819768PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00481 Pfam IPR001932 PPM-type phosphatase domain 24 273 6
SM00332 SMART IPR001932 PPM-type phosphatase domain 13 286 7
PS51746 Prosite Profiles IPR001932 PPM-type phosphatase domain 23 288 7
PS01032 Prosite Patterns IPR000222 PPM-type phosphatase, divalent cation binding 57 65 4
PTHR13832:SF102 HMMPANTHER 1 308 2
PTHR13832 HMMPANTHER IPR015655 Protein phosphatase 2C family 1 308 5
3.60.40.10 Gene3D IPR001932 PPM-type phosphatase domain 11 296 7
SSF81606 SuperFamily IPR001932 PPM-type phosphatase domain 3 288 7

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.35 Da
Charge -21.50
Codon Adaptation Index 0.46
Isoelectric point 4.49
Molecular weight 44.86 kDa
Number of residues 414
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phaseS390
present during mitotic M phaseS389
present during mitotic M phaseS391
S389
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S390 PMID:21712547
present during mitotic M phase experimental evidence S389 PMID:21712547
present during mitotic M phase experimental evidence S391 PMID:21712547
experimental evidence S389 PMID:24763107
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for ptc3 (SPAC2G11.07c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
43406during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
43460during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
41820during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
43823during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
27346.25during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
45537during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
28701.24during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
13during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
4.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2506
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC2G11.07c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bypaa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC2G11.07c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
phenotype enhanced byptc1protein phosphatase 2C Ptc1 Phenotypic EnhancementPMID:10398679
positive genetic interaction withsut1alpha-glucoside transmembrane transporter Sut1 Positive GeneticPMID:22681890
positive genetic interaction withliz1pantothenate transmembrane transporter Liz1 Positive GeneticPMID:22681890
positive genetic interaction witherg5C-22 sterol desaturase Erg5 Positive GeneticPMID:22681890
positive genetic interaction witharp6actin-like protein Arp6 Positive GeneticPMID:18818364
positive genetic interaction withser2phosphoserine phosphatase Ser2 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withraf2Rik1-associated factor Raf2 Positive GeneticPMID:18818364
positive genetic interaction withhus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
positive genetic interaction withcxr1mRNA processing factor Positive GeneticPMID:22681890
positive genetic interaction withvph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Positive GeneticPMID:18818364
positive genetic interaction withpkp1mitochondrial pyruvate dehydrogenase (lipoamide) kinase Pkp1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPBC16H5.13WD repeat protein, human WDR7 ortholog Positive GeneticPMID:22681890
positive genetic interaction withatb2tubulin alpha 2 Positive GeneticPMID:22681890
positive genetic interaction withsck2serine/threonine protein kinase Sck2 Positive GeneticPMID:24463365
positive genetic interaction withswc5Swr1 complex subunit Swc5 Positive GeneticPMID:18818364
positive genetic interaction withski2Ski complex RNA helicase Ski2 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withSPBC1861.07elongin C (predicted) Positive GeneticPMID:18818364
positive genetic interaction withphp5CCAAT-binding factor complex subunit Php5 Positive GeneticPMID:22681890
negative genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
negative genetic interaction withcdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
negative genetic interaction withgcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
negative genetic interaction withpyp1tyrosine phosphatase Pyp1 Negative GeneticPMID:22681890
negative genetic interaction withamk2AMP-activated protein kinase beta subunit Amk2 Negative GeneticPMID:22681890
negative genetic interaction withSPBPB2B2.18Schizosaccharomyces specific protein Negative GeneticPMID:22681890
negative genetic interaction withmug113T5orf172 family protein Negative GeneticPMID:22681890
negative genetic interaction withmga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Negative GeneticPMID:18818364
negative genetic interaction withmpr1histidine-containing response regulator phosphotransferase Mpr1 Negative GeneticPMID:22681890
negative genetic interaction withymr1phosphatidylinositol-3-phosphatase, myotubularin family (predicted) Negative GeneticPMID:18818364
negative genetic interaction withddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:18818364
negative genetic interaction withypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:22681890
negative genetic interaction withatp11mitochondrial F1-ATPase chaperone Atp11 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withreb1RNA polymerase I transcription termination factor/ RNA polymerase II transcription factor Reb1 Negative GeneticPMID:22681890
negative genetic interaction withfar10SIP/FAR complex FHA domain subunit Far10/Csc1 Negative GeneticPMID:22681890
negative genetic interaction withalp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
negative genetic interaction withmid1medial ring protein Mid1 Negative GeneticPMID:22681890
negative genetic interaction withiqw1WD repeat protein, Iqw1 Negative GeneticPMID:22681890
negative genetic interaction withvps3CORVET complex subunit, GTPase regulator Vps3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withvps5retromer complex subunit Vps5 Negative GeneticPMID:22681890
negative genetic interaction withpar1protein phosphatase PP2A regulatory subunit B-56 Par1 Negative GeneticPMID:22681890
negative genetic interaction withrpl430260S ribosomal protein L37a (predicted) Negative GeneticPMID:22681890
negative genetic interaction withset1histone lysine methyltransferase Set1 Negative GeneticPMID:22681890
negative genetic interaction witharp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
negative genetic interaction withemc1ER membrane protein complex subunit Emc1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpmp1dual-specificity MAP kinase phosphatase Pmp1 Negative GeneticPMID:22681890
negative genetic interaction withsfp1transcription factor Sfp1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:18818364
negative genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
negative genetic interaction withSPCP1E11.10ankyrin repeat protein, unknown biological role Negative GeneticPMID:22681890
negative genetic interaction withgcn20AAA family ATPase Gcn20 (predicted) Negative GeneticPMID:22681890
synthetically rescued bywis1MAP kinase kinase Wis1 Synthetic RescuePMID:7859738
enhances phenotype ofptc1protein phosphatase 2C Ptc1 Phenotypic EnhancementPMID:17881729
overexpression rescuesppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Dosage RescuePMID:17881729
overexpression rescuespck2protein kinase C (PKC)-like Pck2 Dosage RescuePMID:17881729
synthetic growth defect withpyp1tyrosine phosphatase Pyp1 Synthetic Growth DefectPMID:9211944
synthetic growth defect withptc2protein phosphatase 2C Ptc2 Synthetic Growth DefectPMID:7859738
synthetic growth defect withptc1protein phosphatase 2C Ptc1 Synthetic Growth DefectPMID:7859738
External References
Database Identifier Description
NBRP SPAC2G11.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC2G11.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC2G11.07c BioGRID Interaction Datasets
Expression Viewer SPAC2G11.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC2G11.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC2G11.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC2G11.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC2G11.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC2G11.07c Transcriptome Viewer (Bähler Lab)
GEO SPAC2G11.07c GEO profiles
PInt SPAC2G11.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC2G11.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC2G11.07c Fission yeast phenotypic data & analysis
Cyclebase SPAC2G11.07c.1 Cell Cycle Data
IntEnz3.1.3.16Integrated relational Enzyme database
Rhea3.1.3.16Annotated reactions database
SPD / RIKEN25/25A02Orfeome Localization Data
UniProtKB/SwissProtQ09173Protein phosphatase 2C homolog 3
ModBaseQ09173Database of comparative protein structure models
STRINGQ09173Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593087protein phosphatase 2C
RefSeq mRNANM_001018485972h- protein phosphatase 2C (ptc3), mRNA

Literature for ptc3

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016