rqh1 (SPAC2G11.12)


Gene Standard Namerqh1 Characterisation Statuspublished
Systematic IDSPAC2G11.12 Feature Typeprotein coding
Synonymshus2, rad12, rec9 Name DescriptionHydroxy Urea Sensitive
ProductRecQ type DNA helicase Rqh1 Product Size1328aa, 149.65 kDa
Genomic Location Chromosome I, 831918-836094 (4177nt); CDS:832027-836013 (3987nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037558
GO:0043140ATP-dependent 3'-5' DNA helicase activityIDAPMID:1272442610
IDAPMID:12478586
GO:0003677DNA bindingIEAUniProtKB-KW:KW-0238GO_REF:0000037385
GO:0005515protein binding865
N_termIPItop3PMID:15702347
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032508DNA duplex unwindingIDAPMID:1272442632
GO:1902971DNA duplex unwinding involved in mitotic DNA replicationISOSGD:S000004802GO_REF:00000241
GO:0006974cellular response to DNA damage stimulusIMPPMID:9372918213
GO:0000724double-strand break repair via homologous recombination31
acts_upstream_ofIGIrad51PMID:12409469
same_pathwayIGIrad52PMID:8709952
same_pathwayIGIrad21PMID:8709952
GO:0031573intra-S DNA damage checkpointIDAPMID:1903710116
GO:0043007maintenance of rDNAIMPPMID:145280106
IMPPMID:16303848
GO:0045950negative regulation of mitotic recombinationIMPPMID:91842152
GO:0006301postreplication repairIMPPMID:1272442617
GO:0000725recombinational repair36
same_pathwayIGIrad57, fbh1PMID:16135799
GO:1903221regulation of mitotic recombination involved in replication fork processingIMPPMID:230939421
GO:0034065replication fork processing at rDNA locusIGIreb1PMID:163038483
GO:0071140resolution of mitotic recombination intermediatesIMPPMID:158891461
GO:0000723telomere maintenanceIGItaz1, ssb1PMID:1742906444
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0031422RecQ helicase-Topo III complexIDAPMID:127244263
GO:0000228nuclear chromosomeIDAPMID:12724426431
GO:0043596nuclear replication forkICGO:0031422GO_REF:000000164
GO:0005730nucleolusIDAPMID:12724426357
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assayrqh1ΔNullPECO:0000005, PECO:0000137, PECO:0000102PMID:20661445119
FYPO:0000268sensitive to UV during vegetative growthCell growth assayrec9-104Not specifiedPECO:0000137PMID:2806887120
Cell growth assayhus2-22 (W789->stop)Not specifiedPMID:12724426
Cell growth assayK/R (K547R)Not specifiedPMID:12724426
Cell growth assayrad12-502 (T543I)Not specifiedPMID:12724426
Cell growth assayK/A (K547A)Not specifiedPMID:12724426
Cell growth assayrqh1ΔNullPMID:12724426
FYPO:0000085sensitive to camptothecinCell growth assayrqh1ΔNullPECO:0000005, PECO:0000137, PECO:0000102PMID:20661445211
FYPO:0000102sensitive to cisplatinCell growth assayrqh1ΔNullPMID:2206447718
FYPO:0000088sensitive to hydroxyurea513
expressivity FYPO_EXT:0000001Cell Growth Assayrqh1ΔNullPECO:0000137, PECO:0000102PMID:23697806
Cell growth assaynot recorded (unrecorded)PMID:10835372
Cell growth assayrqh1ΔNullPECO:0000005, PECO:0000137, PECO:0000102PMID:20661445
FYPO:0000267sensitive to ionizing radiationCell growth assayrad12-502 (T543I)Not specifiedPMID:1272442644
Cell growth assayrqh1ΔNullPMID:12724426
FYPO:0000089sensitive to methyl methanesulfonateCell growth assayrec9-104Not specifiedPECO:0000137PMID:2806887214
FYPO:0002060viable vegetative cell populationMicroscopynot recorded (unrecorded)PMID:91842153751
Microscopyrqh1ΔNullPMID:20473289
Cell growth assayrqh1ΔNullPMID:12724426
Microscopyrqh1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000007abnormal S-phase DNA damage checkpointrqh1ΔNullPMID:190371019
FYPO:0000029abnormal chromosome segregationnot recorded (unrecorded)PMID:9184215178
FYPO:0000059abnormal mitotic cell cycleMicroscopyrqh1ΔNullPECO:0000005, PECO:0000137PMID:23697806640
FYPO:0000670abnormal mitotic chromosome separationMicroscopyrqh1ΔNullPMID:2066144533
FYPO:0002101abolished ATP-dependent 3'-5' DNA helicase activityEnzyme assay dataK/A (K547A)Not specifiedPMID:127244261
Enzyme assay dataK/R (K547R)Not specifiedPMID:12724426
Enzyme assay datarad12-502 (T543I)Not specifiedPMID:12724426
FYPO:0001799cell cycle arrest in mitotic G2 phase during response to DNA damageCell growth assayrqh1ΔNullPMID:127244262
FYPO:0002054cut during cellular response to hydroxyurea38
penetrance FYPO_EXT:0000002Microscopyrqh1ΔNullPMID:23697806
FYPO:0003179decreased intragenic meiotic recombination19
affecting ade6Cell growth assayrec9-104Not specifiedPMID:2806887
FYPO:0001122elongated vegetative cell663
penetrance FYPO_EXT:0000003Microscopyrqh1ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0003584increased double-strand break repair via nonhomologous end joiningOtherrqh1ΔNullPMID:103735823
FYPO:0000274increased duration of mitotic M phasenot recorded (unrecorded)PMID:163038484
FYPO:0003588increased gross chromosomal rearrangement during replication fork processingGel electrophoresis evidencerqh1ΔNullPMID:230939421
FYPO:0002604increased histone H2A phosphorylation at rDNAChromatin immunoprecipitation experimentrqh1ΔNullPMID:206614452
FYPO:0002102normal DNA damage checkpoint during cellular response to UVCell growth assayrqh1ΔNullPMID:127244264
FYPO:0000833normal protein level38
affecting rqh1Western blot assayrad12-502 (T543I)Not specifiedPMID:12724426
FYPO:0002239shortened telomeresGel electrophoresis evidencerqh1ΔNullPMID:1037358229
FYPO:0001491viable vegetative cellCell growth assayrqh1ΔNullPMID:127244263599
FYPO:0002104viable vegetative cell with normal cell shape19
penetrance FYPO_EXT:0000001Microscopyrqh1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in rqh1 RecQ type DNA helicase Rqh1 PMID:12724426
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1831918836094
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00570 Pfam IPR002121 HRDC domain 1120 1185 2
PF00270 Pfam IPR011545 DNA/RNA helicase, DEAD/DEAH box type, N-terminal 522 695 43
PF00271 Pfam IPR001650 Helicase, C-terminal 762 838 68
PF09382 Pfam IPR018982 RQC domain 924 1033 1
SM00490 SMART IPR001650 Helicase, C-terminal 756 838 68
SM00956 SMART IPR018982 RQC domain 924 1035 1
SM00487 SMART IPR014001 Helicase, superfamily 1/2, ATP-binding domain 516 723 69
SM00341 SMART IPR002121 HRDC domain 1115 1195 2
PS51192 Prosite Profiles IPR014001 Helicase, superfamily 1/2, ATP-binding domain 528 707 69
PS51194 Prosite Profiles IPR001650 Helicase, C-terminal 728 876 69
PS50967 Prosite Profiles IPR002121 HRDC domain 1115 1195 2
PS00690 Prosite Patterns IPR002464 DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site 646 655 10
PTHR13710:SF13 HMMPANTHER 68 1197 1
PTHR13710 HMMPANTHER 68 1197 4
3.40.50.300 Gene3D 712 850 288
1.10.150.80 Gene3D IPR002121 HRDC domain 1118 1194 1
3.40.50.300 Gene3D 500 711 288
1.10.10.10 Gene3D IPR011991 Winged helix-turn-helix DNA-binding domain 851 1034 46
SSF47819 SuperFamily IPR010997 HRDC-like 1120 1196 4
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 713 967 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 812 854 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 495 670 294
Coil ncoils Rabaptin coiled-coil domain 866 887 968
TIGR00614 tigrfam IPR004589 DNA helicase, ATP-dependent, RecQ type 510 969 1

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000174DEAD/DEAH box helicaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000174
PBO:0000619RecQ familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000619
PBO:0011078Req family helicaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0011078

Protein Properties

Ave. residue weight 112.69 Da
Charge 11.00
Isoelectric point 7.17
Molecular weight 149.65 kDa
Number of residues 1328
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
NDduring GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.5during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.47during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancer142
Bloom's syndrome1
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in bacteria1002
conserved in metazoa3428
conserved in vertebrates3402
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
swi2Swi5 complex subunit Swi2 Synthetic Growth DefectPMID:18045993
slx1structure-specific endonuclease catalytic subunit Synthetic LethalityPMID:16710300
Synthetic LethalityPMID:14528010
cdc1DNA polymerase delta small subunit Cdc1 Synthetic LethalityPMID:9891047
rhp18Rad18 homolog Rhp18 Synthetic Growth DefectPMID:11523791
hta1histone H2A alpha Synthetic Growth DefectPMID:20661445
Synthetic Growth DefectPMID:15226425
exo1exonuclease I Exo1 Phenotypic EnhancementPMID:21931565
Synthetic RescuePMID:23297345
pot1telomere end-binding protein Pot1 Synthetic LethalityPMID:21098121
Phenotypic Suppression
Synthetic Growth Defect
Phenotypic EnhancementPMID:18722173
Phenotypic EnhancementPMID:23297345
Synthetic Lethality
swi5Swi5 protein Phenotypic SuppressionPMID:23828040
Synthetic RescuePMID:15802523
cdc6DNA polymerase delta catalytic subunit Cdc6 Synthetic LethalityPMID:9891047
pku80Ku domain protein Pku80 Phenotypic EnhancementPMID:19682091
brc1BRCT domain protein Brc1 Synthetic Growth DefectPMID:20661445
Synthetic Growth DefectPMID:15972456
top3DNA topoisomerase III Synthetic RescuePMID:12235386
Synthetic RescuePMID:10497270
Synthetic RescuePMID:23516381
Synthetic Growth DefectPMID:15702347
Synthetic RescuePMID:12724426
Synthetic Growth DefectPMID:10572171
Synthetic Rescue
rdl1RAD51D-like protein 1 Synthetic RescuePMID:16710300
fbh1DNA helicase I Synthetic LethalityPMID:16135800
Synthetic LethalityPMID:16135799
cdc17ATP-dependent DNA replication ligase Cdc17 Synthetic LethalityPMID:9891047
tor1phosphatidylinositol kinase Tor1 Synthetic Growth DefectPMID:23703609
rad9checkpoint clamp complex protein Rad9 Phenotypic SuppressionPMID:9566891
pol1DNA polymerase alpha catalytic subunit Synthetic LethalityPMID:9891047
ufd1Cdc48-Ufd1-Npl4 complex subunit Ufd1 involved in ubiquitin-dependent protein catabolism (predicted) Synthetic Growth DefectPMID:24265825
sws1SWIM domain containing-Srs2 interacting protein 1 Synthetic RescuePMID:16710300
top1DNA topoisomerase I Synthetic RescuePMID:12084712
cdc20DNA polymerase epsilon catalytic subunit Pol2 Phenotypic EnhancementPMID:18667534
Synthetic LethalityPMID:9891047
cdc23MCM-associated protein Mcm10 Synthetic LethalityPMID:9891047
slx8SUMO-targeted ubiquitin-protein ligase E3 Slx8 Synthetic LethalityPMID:17762865
rad60DNA repair protein Rad60 Synthetic LethalityPMID:16880212
Dosage Lethality
Synthetic LethalityPMID:16354704
hta2histone H2A beta Synthetic Growth DefectPMID:20661445
Synthetic Growth DefectPMID:15226425
rad55RecA family ATPase Rad55/Rhp55 Synthetic RescuePMID:16135800
Synthetic RescuePMID:15802523
Synthetic RescuePMID:14993467
crb2DNA repair protein Rad9 homolog, Rhp9 Synthetic Growth DefectPMID:12589755
Synthetic RescuePMID:12023299
Synthetic Growth DefectPMID:10373582
hrq1RecQ type DNA helicase Hrq1 (predicted) Phenotypic EnhancementPMID:22064477
Synthetic Growth Defect
orc1origin recognition complex subunit Orc1 Phenotypic EnhancementPMID:18667534
rad3ATR checkpoint kinase Rad3 Synthetic LethalityPMID:17178839
Synthetic Growth DefectPMID:17893680
hsk1Dbf4(Dfp1)-dependent protein kinase Hsk1 Synthetic LethalityPMID:11027263
cdc27DNA polymerase delta subunit Cdc27 Synthetic LethalityPMID:9891047
swi1replication fork protection complex subunit Swi1 Synthetic Growth DefectPMID:16710300
Synthetic Growth DefectPMID:18045993
Synthetic Growth DefectPMID:20176980
mus81Holliday junction resolvase subunit Mus81 Synthetic LethalityPMID:16710300
Synthetic LethalityPMID:12084712
rrp1ATP-dependent DNA helicase (predicted) Synthetic Growth DefectPMID:23828040
swi3replication fork protection complex subunit Swi3 Synthetic LethalityPMID:16710300
Synthetic LethalityPMID:18045993
Synthetic Growth DefectPMID:20176980
mcl1DNA polymerase alpha accessory factor Mcl1 Synthetic LethalityPMID:12455694
Synthetic LethalityPMID:15643072
rad52DNA recombination protein Rad52 (previously Rad22) Synthetic Growth DefectPMID:11452021
Phenotypic EnhancementPMID:20705238
Synthetic LethalityPMID:10373582
cut8tethering factor for nuclear proteasome Cut8 Synthetic LethalityPMID:17178839
slx4structure-specific endonuclease subunit Slx4 Synthetic LethalityPMID:16710300
Synthetic LethalityPMID:14528010
ctf18RFC-like complex subunit Ctf18 Phenotypic EnhancementPMID:18045993
chk1Chk1 protein kinase Phenotypic EnhancementPMID:9372918
Phenotypic SuppressionPMID:23584455
Phenotypic SuppressionPMID:21098121
Phenotypic EnhancementPMID:15226425
srs2ATP-dependent DNA helicase, UvrD subfamily Synthetic Growth DefectPMID:17893680
Synthetic Growth DefectPMID:11452021
Synthetic Growth DefectPMID:16710300
Synthetic Growth DefectPMID:14993467
Synthetic Growth DefectPMID:12409469
rad2FEN-1 endonuclease Rad2 Phenotypic EnhancementPMID:7623848
Synthetic LethalityPMID:9891047
mad2spindle checkpoint protein Mad2 Synthetic Growth DefectPMID:16303848
eme1Holliday junction resolvase subunit Eme1 Synthetic Growth DefectPMID:23584455
trt1telomerase reverse transcriptase 1 protein Trt1 Synthetic Growth DefectPMID:22313747
Phenotypic Enhancement
mcm7MCM complex subunit Mcm7 Synthetic Growth DefectPMID:11606526
nse6Smc5-6 complex non-SMC subunit Nse6 Synthetic LethalityPMID:16478984
reb1RNA polymerase I transcription termination factor Reb1 Synthetic Growth DefectPMID:16303848
rad57RecA family ATPase Rad57/Rhp57 Synthetic RescuePMID:16135799
Synthetic Growth DefectPMID:23828040
Phenotypic Suppression
Synthetic RescuePMID:14993467
Synthetic RescuePMID:16354704
Synthetic RescuePMID:23297345
rrp2ATP-dependent DNA helicase (predicted) Synthetic Growth DefectPMID:23828040
sfr1Swi five-dependent recombination repair protein Sfr1 Phenotypic SuppressionPMID:18769921
rad51RecA family recombinase Rad51/Rhp51 Synthetic RescuePMID:16135799
Synthetic RescuePMID:14993467
Synthetic RescuePMID:16135800
Synthetic RescuePMID:16354704
Synthetic LethalityPMID:15802523
Phenotypic SuppressionPMID:23297345
Synthetic Rescue
Synthetic Growth DefectPMID:20705238
Synthetic Growth DefectPMID:23828040
rad54DNA-dependent ATPase Rad54/Rhp54 Synthetic LethalityPMID:15802523
uve1endonuclease Uve1 Phenotypic EnhancementPMID:9372918
Phenotypic EnhancementPMID:9092661
mus7DNA repair protein Mus7/Mms22 Phenotypic EnhancementPMID:17660542
Synthetic Growth DefectPMID:17307401
cdc13G2/M B-type cyclin Cdc13 Synthetic RescuePMID:12023299
nse4Smc5-6 complex non-SMC subunit Nse4 Synthetic LethalityPMID:15485909
rad13DNA repair nuclease Rad13 Phenotypic EnhancementPMID:9372918
Phenotypic EnhancementPMID:9092661
Phenotypic EnhancementPMID:7623848
mcm4MCM complex subunit Mcm4/Cdc21 Synthetic LethalityPMID:11606526
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
pli1SUMO E3 ligase Pli1 Biochemical ActivityPMID:18031226
ssb1DNA replication factor A subunit Ssb1 Affinity Capture-WesternPMID:17429064
top3DNA topoisomerase III Affinity Capture-WesternPMID:15702347
Affinity Capture-WesternPMID:12724426
nse2Smc5-6 complex non-SMC subunit 2, SUMO ligase Biochemical ActivityPMID:18031226
top1DNA topoisomerase I Affinity Capture-WesternPMID:15702347
External References
Database Identifier Description
NBRP SPAC2G11.12 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC2G11.12 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC2G11.12 BioGRID Interaction Datasets
Expression Viewer SPAC2G11.12 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC2G11.12 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC2G11.12 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC2G11.12 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC2G11.12 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC2G11.12 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC2G11.12 Cell Cycle Data
GEO SPAC2G11.12 GEO profiles
PInt SPAC2G11.12 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC2G11.12 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN39/39D09Orfeome Localization Data
UniProtKB/SwissProtQ09811ATP-dependent DNA helicase hus2/rqh1
ModBaseQ09811Database of comparative protein structure models
STRINGQ09811Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593092RecQ type DNA helicase Rqh1
RefSeq mRNANM_001018490972h- RecQ type DNA helicase Rqh1 (rqh1), mRNA
European Nucleotide ArchiveCAA70577.1ENA Protein Mapping
European Nucleotide ArchiveCAA91177.1ENA Protein Mapping
UniParcUPI000012CE81UniProt Archive

Literature for rqh1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014