rqh1 (SPAC2G11.12)

Gene Standard Namerqh1 ChromosomeI
Systematic IDSPAC2G11.12 Gene Start831918
Synonymshus2, rad12, rec9 Gene End836094
ProductRecQ type DNA helicase Rqh1 Gene Length4177
Feature Typeprotein coding CDS Start832027
Name DescriptionHydroxy Urea Sensitive CDS End836013
Characterisation Statuspublished CDS Length3987

Ensembl Gene Location
  1. SPAC2G11.10c
  2. SPNCRNA.689
  3. prh1
  4. SPNCRNA.690
  5. rqh1
  6. atg22
  7. SPNCRNA.691
  8. taf111
  9. tgs1
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000007abnormal S-phase DNA damage checkpointrqh1delta (deletion)deletionPMID:19037101
FYPO:0000029abnormal chromosome segregationno_name (unrecorded)unrecordedPMID:9184215
FYPO:0000088sensitive to hydroxyureano_name (unrecorded)unrecordedPMID:10835372
FYPO:0000102sensitive to cisplatinCell growth assayrqh1delta (deletion)deletionPMID:22064477
FYPO:0000274increased duration of mitotic M phaseno_name (unrecorded)unrecordedPMID:16303848
FYPO:0002060viable vegetative cell populationMicroscopyrqh1delta (deletion)deletionPMID:20473289
no_name (unrecorded)unrecordedPMID:9184215
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0003677DNA bindingIEAUniProtKB:KW-0238GO_REF:0000037
GO:0005515protein binding
N_termIPItop3PMID:15702347
GO:0005524ATP bindingICGO:0043140GO_REF:0000001
GO:0043140ATP-dependent 3'-5' DNA helicase activityIDAPMID:12478586
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0000723telomere maintenanceIMPPMID:10373582
IGItaz1, ssb1PMID:17429064
GO:0000724double-strand break repair via homologous recombination
same_pathwayIGIrad22PMID:8709952
acts_upstream_ofIGIrad51PMID:12409469
same_pathwayIGIrad21PMID:8709952
GO:0000725recombinational repair
same_pathwayIGIrad57, fbh1PMID:16135799
GO:0006268DNA unwinding involved in replicationISOSGD:S000004802GO_REF:0000024
GO:0006974response to DNA damage stimulusIMPPMID:9372918
GO:0007131reciprocal meiotic recombinationIMPPMID:2806887
GO:0031573intra-S DNA damage checkpointIDAPMID:19037101
GO:0034065replication fork processing at rDNA locusIGIreb1PMID:16303848
GO:0043007maintenance of rDNAIMPPMID:16303848
IMPPMID:14528010
GO:0045950negative regulation of mitotic recombinationIMPPMID:9184215
GO:0071140resolution of mitotic recombination intermediatesIMPPMID:15889146
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0031422RecQ helicase-Topo III complexIDAPMID:12724426
GO:0043596nuclear replication forkICGO:0031422GO_REF:0000001
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1831918836094

References

Region Start End Reference
three_prime_UTR836014836094PMID:21511999
three_prime_UTR836014836094PMID:21511999
five_prime_UTR831918832026PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF09382 IPR018982 Pfam RQC domain 924 1033 1
PF00271 IPR001650 Pfam Helicase, C-terminal 762 838 69
PF00570 IPR002121 Pfam HRDC domain 1120 1185 2
PF00270 IPR011545 Pfam DNA/RNA helicase, DEAD/DEAH box type, N-terminal 522 695 43
SM00487 IPR014001 SMART Helicase, superfamily 1/2, ATP-binding domain 516 723 69
SM00490 IPR001650 SMART Helicase, C-terminal 756 838 67
SM00956 IPR018982 SMART RQC domain 924 1035 1
SM00341 IPR002121 SMART HRDC domain 1115 1195 2
PS00690 IPR002464 Prosite Patterns DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site 646 655 10
PS51194 IPR001650 Prosite Profiles Helicase, C-terminal 728 876 69
PS51192 IPR014001 Prosite Profiles Helicase, superfamily 1/2, ATP-binding domain 528 707 69
PS50967 IPR002121 Prosite Profiles HRDC domain 1115 1195 2
G3DSA:1.10.150.80 IPR015906 Gene3D 1118 1194 1
G3DSA:3.40.50.300 Gene3D 500 711 279
G3DSA:3.40.50.300 Gene3D 712 850 279
G3DSA:1.10.10.10 IPR011991 Gene3D 851 1034 43
SSF47819 IPR010997 SuperFamily 1120 1196 4
SSF52540 SuperFamily 812 854 294
SSF52540 SuperFamily 713 967 294
SSF52540 SuperFamily 495 670 294
PTHR13710 hmmpanther 15 1198 4
PTHR13710:SF13 hmmpanther 15 1198 1
Coil ncoils Rabaptin coiled-coil domain 866 887 974
TIGR00614 IPR004589 tigrfam DNA helicase, ATP-dependent, RecQ type 510 969 1

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceAll Genes
PBO:0000174DEAD/DEAH box helicaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000174
PBO:0000619RecQ familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000619
PBO:0011078Req family helicaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0011078

Protein Properties

Ave. residue weight 112.69 Da
Charge 11.00
Isoelectric point 7.17
Molecular weight 149.65 kDa
Number of residues 1328
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127		population_widePMID:23101633
experimental evidencesingle-celled organism vegetative growth phasePECO:0000005,
PECO:0000014		population_widePMID:23101633
RNA levelexperimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide1.5PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide0.47PMID:23101633
Disease Association
DescriptionQualifierReference
cancer
Bloom's syndrome
Species Distribution
DescriptionQualifierReference
predominantly single copy (one to one)
conserved in fungi
conserved in eukaryotes
conserved in bacteria
conserved in metazoa
conserved in vertebrates
conserved in archaea
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
srs2ATP-dependent DNA helicase, UvrD subfamily Synthetic Growth DefectPMID:14993467
Synthetic Growth DefectPMID:11452021
Synthetic Growth DefectPMID:12409469
Synthetic Growth DefectPMID:16710300
Synthetic Growth DefectPMID:17893680
rdl1RAD51D-like protein 1 Synthetic RescuePMID:16710300
rad13DNA repair nuclease Rad13 Phenotypic EnhancementPMID:9372918
Phenotypic EnhancementPMID:7623848
Phenotypic EnhancementPMID:9092661
slx1structure-specific endonuclease catalytic subunit Synthetic LethalityPMID:14528010
Synthetic LethalityPMID:16710300
pol1DNA polymerase alpha catalytic subunit Synthetic LethalityPMID:9891047
trt1telomerase reverse transcriptase 1 protein Trt1 Synthetic Growth DefectPMID:22313747
Phenotypic Enhancement
orc1origin recognition complex subunit Orc1 Phenotypic EnhancementPMID:18667534
rad22DNA recombination protein Rad22 (previously Rad52) Synthetic Growth DefectPMID:11452021
Phenotypic EnhancementPMID:20705238
sws1SWIM domain containing-Srs2 interacting protein 1 Synthetic RescuePMID:16710300
rad2FEN-1 endonuclease Rad2 Synthetic LethalityPMID:9891047
Phenotypic EnhancementPMID:7623848
pku80Ku domain protein Pku80 Phenotypic EnhancementPMID:19682091
cdc23MCM-associated protein Mcm10 Synthetic LethalityPMID:9891047
cdc13G2/M B-type cyclin Cdc13 Synthetic RescuePMID:12023299
slx4structure-specific endonuclease subunit Slx4 Synthetic LethalityPMID:14528010
Synthetic LethalityPMID:16710300
sfr1Swi five-dependent recombination repair protein Sfr1 Phenotypic SuppressionPMID:18769921
mus81Holliday junction resolvase subunit Mus81 Synthetic LethalityPMID:12084712
Synthetic LethalityPMID:16710300
mus7DNA repair protein Mus7/Mms22 Synthetic Growth DefectPMID:17307401
Phenotypic EnhancementPMID:17660542
cdc20DNA polymerase epsilon catalytic subunit Pol2 Synthetic LethalityPMID:9891047
Phenotypic EnhancementPMID:18667534
mcl1DNA polymerase alpha accessory factor Mcl1 Synthetic LethalityPMID:15643072
Synthetic LethalityPMID:12455694
cdc27DNA polymerase delta subunit Cdc27 Synthetic LethalityPMID:9891047
rhp18Rad18 homolog Rhp18 Synthetic Growth DefectPMID:11523791
cut8tethering factor for nuclear proteasome Cut8 Synthetic LethalityPMID:17178839
chk1Chk1 protein kinase Phenotypic EnhancementPMID:9372918
Phenotypic EnhancementPMID:15226425
Phenotypic SuppressionPMID:21098121
hta1histone H2A alpha Synthetic Growth DefectPMID:15226425
Synthetic Growth DefectPMID:20661445
top1DNA topoisomerase I Synthetic RescuePMID:12084712
rad54DNA-dependent ATPase Rad54/Rhp54 Synthetic LethalityPMID:15802523
ctp1CtIP-related endonuclease Synthetic LethalityPMID:21931565
slx8SUMO-targeted ubiquitin-protein ligase E3 Slx8 (predicted) Synthetic LethalityPMID:17762865
nse4Smc5-6 complex non-SMC subunit Nse4 Synthetic LethalityPMID:15485909
nse6Smc5-6 complex non-SMC subunit Nse6 Synthetic LethalityPMID:16478984
mcm7MCM complex subunit Mcm7 Synthetic Growth DefectPMID:11606526
rad55RecA family ATPase Rad55/Rhp55 Synthetic RescuePMID:14993467
Synthetic RescuePMID:16135800
Synthetic RescuePMID:15802523
top3DNA topoisomerase III Synthetic RescuePMID:23516381
Synthetic RescuePMID:12235386
Synthetic RescuePMID:10497270
Synthetic Growth DefectPMID:15702347
Synthetic RescuePMID:12724426
Synthetic RescuePMID:10572171
Synthetic Growth Defect
hta2histone H2A beta Synthetic Growth DefectPMID:15226425
Synthetic Growth DefectPMID:20661445
reb1RNA polymerase I transcription termination factor Reb1 Synthetic Growth DefectPMID:16303848
mre11Mre11 nuclease Synthetic LethalityPMID:21931565
mcm4MCM complex subunit Mcm4/Cdc21 Synthetic LethalityPMID:11606526
rad9checkpoint clamp complex protein Rad9 Phenotypic SuppressionPMID:9566891
brc1BRCT domain protein Brc1 Synthetic Growth DefectPMID:15972456
Synthetic Growth DefectPMID:20661445
crb2DNA repair protein Rad9 homolog, Rhp9 Synthetic Growth DefectPMID:12589755
Synthetic RescuePMID:12023299
uve1endonuclease Uve1 Phenotypic EnhancementPMID:9372918
Phenotypic EnhancementPMID:9092661
rad3ATR checkpoint kinase Rad3 Synthetic LethalityPMID:17178839
Synthetic Growth DefectPMID:17893680
cdc1DNA polymerase delta small subunit Cdc1 Synthetic LethalityPMID:9891047
rad60DNA repair protein Rad60 Synthetic LethalityPMID:16354704
Dosage LethalityPMID:16880212
Synthetic Lethality
rad51RecA family recombinase Rad51/Rhp51 Synthetic RescuePMID:14993467
Synthetic RescuePMID:16354704
Synthetic RescuePMID:16135800
Synthetic Growth DefectPMID:20705238
Synthetic LethalityPMID:15802523
Phenotypic SuppressionPMID:23297345
Synthetic Rescue
Synthetic RescuePMID:16135799
swi1replication fork protection complex subunit Swi1 Synthetic Growth DefectPMID:18045993
Synthetic Growth DefectPMID:20176980
Synthetic Growth DefectPMID:16710300
mad2spindle checkpoint protein Mad2 Synthetic Growth DefectPMID:16303848
cdc17ATP-dependent DNA ligase Cdc17 Synthetic LethalityPMID:9891047
swi2Swi5 complex subunit Swi2 Synthetic Growth DefectPMID:18045993
fbh1DNA helicase I Synthetic LethalityPMID:16135800
Synthetic LethalityPMID:16135799
hsk1Dbf4(Dfp1)-dependent protein kinase Hsk1 Synthetic LethalityPMID:11027263
rad57RecA family ATPase Rad57/Rhp57 Synthetic RescuePMID:14993467
Synthetic RescuePMID:16354704
Synthetic RescuePMID:23297345
Synthetic RescuePMID:16135799
ctf18RFC-like complex subunit Ctf18 Phenotypic EnhancementPMID:18045993
hrq1RecQ type DNA helicase Hrq1 (predicted) Synthetic Growth DefectPMID:22064477
Phenotypic Enhancement
cdc6DNA polymerase delta catalytic subunit Cdc6 Synthetic LethalityPMID:9891047
exo1exonuclease I Exo1 Phenotypic EnhancementPMID:21931565
Synthetic RescuePMID:23297345
swi5Swi5 protein Synthetic RescuePMID:15802523
swi3replication fork protection complex subunit Swi3 Synthetic LethalityPMID:18045993
Synthetic LethalityPMID:16710300
Synthetic Growth DefectPMID:20176980
pot1telomere end-binding protein Pot1 Synthetic LethalityPMID:23297345
Phenotypic Enhancement
Phenotypic EnhancementPMID:18722173
Synthetic LethalityPMID:21098121
Phenotypic Suppression
Synthetic Growth Defect
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
pli1SUMO E3 ligase Pli1 Biochemical ActivityPMID:18031226
nse2Smc5-6 complex non-SMC subunit 2 Biochemical ActivityPMID:18031226
top3DNA topoisomerase III Affinity Capture-WesternPMID:15702347
Affinity Capture-WesternPMID:12724426
ssb1DNA replication factor A subunit Ssb1 Affinity Capture-WesternPMID:17429064
top1DNA topoisomerase I Affinity Capture-WesternPMID:15702347
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External References
Database Identifier Description
NBRP SPAC2G11.12 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC2G11.12 Retrieval of eukaryotic orthologs
BioGrid SPAC2G11.12 BioGRID Interaction Datasets
Bähler Lab SPAC2G11.12 Cell Cycle Expression Profile
Bähler Lab SPAC2G11.12 Meiosis/Sporulation Expression Profies
Bähler Lab SPAC2G11.12 Pheromone response/mating expression profiles
Bähler Lab SPAC2G11.12 Environmental stress expression profiles
Bähler Lab SPAC2G11.12 Bähler Lab Transcriptome Viewer
Cyclebase SPAC2G11.12 Cell Cycle Data
PInt SPAC2G11.12 Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPAC2G11.12 Entrez Gene
SPD / RIKEN39/39D09Orfeome Localization Data
WikiGene2541620RecQ type DNA helicase Rqh1
Uniprot_genenameRQH1
EntrezGene2541620RecQ type DNA helicase Rqh1
UniProtKB/SwissProtQ09811ATP-dependent DNA helicase hus2/rqh1
ModBaseQ09811Database of comparative protein structure models
Pfam Protein DomainsQ09811Pfam Domain Arrangement
RefSeq PeptideNP_593092RecQ type DNA helicase Rqh1
RefSeq mRNANM_001018490972h- RecQ type DNA helicase Rqh1 (rqh1), mRNA
European Nucleotide ArchiveCAA70577ENA Protein Mapping
European Nucleotide ArchiveCAA91177ENA Protein Mapping
UniParcUPI000012CE81UniProt Archive

Literature for rqh1
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013