rqh1 (SPAC2G11.12)

Gene Standard Namerqh1 Characterisation Statuspublished
Systematic IDSPAC2G11.12 Feature Typeprotein coding
Synonymshus2, rad12, rec9 Name DescriptionHydroxy Urea Sensitive
ProductRecQ type DNA helicase Rqh1 Product Size1328aa, 149.65 kDa
Genomic Location Chromosome I, 831918-836094 (4177nt); CDS:832027-836013 (3987nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
ATP-dependent 3'-5' DNA helicase activity11
Annotation ExtensionEvidenceWith/FromReference
DNA binding383
Annotation ExtensionEvidenceWith/FromReference
protein binding831
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cellular response to DNA damage stimulus211
Annotation ExtensionEvidenceWith/FromReference
DNA duplex unwinding31
Annotation ExtensionEvidenceWith/FromReference
DNA duplex unwinding involved in mitotic DNA replication1
Annotation ExtensionEvidenceWith/FromReference
double-strand break repair via homologous recombination32
Annotation ExtensionEvidenceWith/FromReference
intra-S DNA damage checkpoint16
Annotation ExtensionEvidenceWith/FromReference
maintenance of rDNA7
Annotation ExtensionEvidenceWith/FromReference
negative regulation of mitotic recombination2
Annotation ExtensionEvidenceWith/FromReference
postreplication repair18
Annotation ExtensionEvidenceWith/FromReference
recombinational repair37
Annotation ExtensionEvidenceWith/FromReference
regulation of mitotic recombination involved in replication fork processing1
Annotation ExtensionEvidenceWith/FromReference
replication fork processing at rDNA locus3
Annotation ExtensionEvidenceWith/FromReference
resolution of mitotic recombination intermediates1
Annotation ExtensionEvidenceWith/FromReference
telomere maintenance48
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nuclear chromosome432
Annotation ExtensionEvidenceWith/FromReference
nuclear replication fork64
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
RecQ helicase-Topo III complex3
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assayrqh1ΔNullPECO:0000005, PECO:0000137, PECO:0000102PMID:20661445147
FYPO:0000085sensitive to camptothecinCell growth assayrqh1ΔNullPECO:0000005, PECO:0000137, PECO:0000102PMID:20661445211
FYPO:0000102sensitive to cisplatinCell growth assayrqh1ΔNullPMID:2206447718
FYPO:0000088sensitive to hydroxyurea514
expressivity FYPO_EXT:0000001Cell growth assayhus2-22 (W789->stop)Not specifiedPMID:1427071
Cell growth assaynot recorded (unrecorded)PMID:10835372
Cell growth assayrqh1ΔNullPECO:0000005, PECO:0000137, PECO:0000102PMID:20661445
expressivity FYPO_EXT:0000001Cell Growth Assayrqh1ΔNullPECO:0000137, PECO:0000102PMID:23697806
FYPO:0000267sensitive to ionizing radiationCell growth assayrad12-502 (T543I)Not specifiedPMID:1272442646
Cell growth assayrqh1ΔNullPMID:12724426
FYPO:0000089sensitive to methyl methanesulfonateCell growth assayrec9-104Not specifiedPECO:0000137PMID:2806887219
FYPO:0000268sensitive to UV during vegetative growthCell growth assayhus2-22 (W789->stop)Not specifiedPMID:12724426123
Cell growth assayrad12-502 (T543I)Not specifiedPMID:12724426
expressivity FYPO_EXT:0000002Cell growth assayhus2-22 (W789->stop)Not specifiedPMID:1427071
Cell growth assayK/A (K547A)Not specifiedPMID:12724426
Cell growth assayrqh1ΔNullPMID:12724426
Cell growth assayK/R (K547R)Not specifiedPMID:12724426
Cell growth assayrec9-104Not specifiedPECO:0000137PMID:2806887
FYPO:0002060viable vegetative cell populationMicroscopyrqh1ΔNullPECO:0000005, PECO:0000137PMID:236978063759
Cell growth assayrqh1ΔNullPMID:12724426
Microscopynot recorded (unrecorded)PMID:9184215

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000029abnormal chromosome segregationnot recorded (unrecorded)PMID:9184215198
FYPO:0000059abnormal mitotic cell cycleMicroscopyrqh1ΔNullPECO:0000005, PECO:0000137PMID:23697806686
FYPO:0000670abnormal mitotic chromosome separationMicroscopyrqh1ΔNullPMID:2066144517
FYPO:0000007abnormal S-phase DNA damage checkpointrqh1ΔNullPMID:1903710111
FYPO:0002101abolished ATP-dependent 3'-5' DNA helicase activityEnzyme assay dataK/R (K547R)Not specifiedPMID:127244261
Enzyme assay dataK/A (K547A)Not specifiedPMID:12724426
Enzyme assay datarad12-502 (T543I)Not specifiedPMID:12724426
FYPO:0001799cell cycle arrest in mitotic G2 phase during response to DNA damageCell growth assayrqh1ΔNullPMID:127244262
FYPO:0002054cut during cellular response to hydroxyurea41
penetrance FYPO_EXT:0000002Microscopyhus2-22 (W789->stop)Not specifiedPECO:0000005, PECO:0000137PMID:1427071
penetrance FYPO_EXT:0000002Microscopyrqh1ΔNullPMID:23697806
FYPO:0003912decreased double-strand break repair via homologous recombinationOtherrqh1ΔNullPMID:251658233
FYPO:0003179decreased intragenic meiotic recombination21
affecting ade6Cell growth assayrec9-104Not specifiedPMID:2806887
FYPO:0001122elongated vegetative cell685
penetrance FYPO_EXT:0000003Microscopyrqh1ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0003584increased double-strand break repair via nonhomologous end joiningOtherrqh1ΔNullPMID:103735823
FYPO:0000274increased duration of mitotic M phasenot recorded (unrecorded)PMID:163038484
FYPO:0003588increased gross chromosomal rearrangement during replication fork processingGel electrophoresis evidencerqh1ΔNullPMID:230939421
FYPO:0002604increased histone H2A phosphorylation at rDNAChromatin immunoprecipitation experimentrqh1ΔNullPMID:206614452
FYPO:0002102normal DNA damage checkpoint during cellular response to UVCell growth assayrqh1ΔNullPMID:127244264
FYPO:0000833normal protein level40
affecting rqh1Western blot assayrad12-502 (T543I)Not specifiedPMID:12724426
FYPO:0002239shortened telomeresGel electrophoresis evidencerqh1ΔNullPMID:1037358233
FYPO:0001491viable vegetative cellCell growth assayrqh1ΔNullPMID:127244263607
FYPO:0002104viable vegetative cell with normal cell shape20
penetrance FYPO_EXT:0000001Microscopyrqh1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00570 Pfam IPR002121 HRDC domain 1120 1185 2
PF00270 Pfam IPR011545 DNA/RNA helicase, DEAD/DEAH box type, N-terminal 522 695 43
PF00271 Pfam IPR001650 Helicase, C-terminal 762 838 68
PF09382 Pfam IPR018982 RQC domain 924 1033 1
SM00490 SMART IPR001650 Helicase, C-terminal 756 838 68
SM00956 SMART IPR018982 RQC domain 924 1035 1
SM00487 SMART IPR014001 Helicase, superfamily 1/2, ATP-binding domain 516 723 69
SM00341 SMART IPR002121 HRDC domain 1115 1195 2
PS51192 Prosite Profiles IPR014001 Helicase, superfamily 1/2, ATP-binding domain 528 707 69
PS51194 Prosite Profiles IPR001650 Helicase, C-terminal 728 876 69
PS50967 Prosite Profiles IPR002121 HRDC domain 1115 1195 2
PS00690 Prosite Patterns IPR002464 DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site 646 655 10
PTHR13710:SF13 HMMPANTHER 68 1197 1
PTHR13710 HMMPANTHER 68 1197 4 Gene3D 712 850 288 Gene3D IPR002121 HRDC domain 1118 1194 1 Gene3D 500 711 288 Gene3D IPR011991 Winged helix-turn-helix DNA-binding domain 851 1034 46
SSF47819 SuperFamily IPR010997 HRDC-like 1120 1196 4
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 713 967 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 812 854 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 495 670 294
Coil ncoils Rabaptin coiled-coil domain 866 887 968
TIGR00614 tigrfam IPR004589 DNA helicase, ATP-dependent, RecQ type 510 969 1

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000174DEAD/DEAH box helicaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000174
PBO:0000619RecQ familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000619
PBO:0011078Req family helicaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0011078

Protein Properties

Ave. residue weight 112.69 Da
Charge 11.00
Isoelectric point 7.17
Molecular weight 149.65 kDa
Number of residues 1328
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.5during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
0.47during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Disease Association
Bloom's syndrome1
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3425
conserved in vertebrates3400
conserved in archaea237

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
nse2Smc5-6 complex non-SMC subunit 2, SUMO ligase Biochemical ActivityPMID:18031226
ssb1DNA replication factor A subunit Ssb1 Affinity Capture-WesternPMID:17429064
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Affinity Capture-WesternPMID:24861625
top3DNA topoisomerase III Affinity Capture-WesternPMID:15702347
Affinity Capture-WesternPMID:12724426
pli1SUMO E3 ligase Pli1 Biochemical ActivityPMID:18031226
top1DNA topoisomerase I Affinity Capture-WesternPMID:15702347
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
swi2Swi5 complex subunit Swi2 Synthetic Growth DefectPMID:18045993
slx1structure-specific endonuclease catalytic subunit Synthetic LethalityPMID:16710300
Synthetic LethalityPMID:14528010
mcm4MCM complex subunit Mcm4/Cdc21 Synthetic LethalityPMID:11606526
cdc1DNA polymerase delta small subunit Cdc1 Synthetic LethalityPMID:9891047
rhp18Rad18 homolog ubiquitin protein ligase E3, Rhp18 Synthetic Growth DefectPMID:11523791
hta1histone H2A alpha Synthetic Growth DefectPMID:20661445
Synthetic Growth DefectPMID:15226425
exo1exonuclease I Exo1 Phenotypic EnhancementPMID:21931565
Synthetic RescuePMID:23297345
pot1telomere end-binding protein Pot1 Phenotypic SuppressionPMID:21098121
Synthetic Lethality
Synthetic Growth Defect
Synthetic LethalityPMID:23297345
Phenotypic Enhancement
Phenotypic EnhancementPMID:18722173
swi5Swi5 protein Phenotypic SuppressionPMID:23828040
Synthetic RescuePMID:15802523
hta2histone H2A beta Synthetic Growth DefectPMID:20661445
Synthetic Growth DefectPMID:15226425
cdc6DNA polymerase delta catalytic subunit Cdc6 Synthetic LethalityPMID:9891047
mad2mitotic spindle checkpoint protein Mad2 Synthetic Growth DefectPMID:16303848
Phenotypic SuppressionPMID:24469396
brc1BRCT domain protein Brc1 Synthetic Growth DefectPMID:20661445
Synthetic Growth DefectPMID:15972456
top3DNA topoisomerase III Synthetic RescuePMID:12235386
Synthetic RescuePMID:10497270
Synthetic RescuePMID:23516381
Synthetic Growth DefectPMID:15702347
Synthetic RescuePMID:12724426
Synthetic Growth DefectPMID:10572171
Synthetic Rescue
rdl1RAD51D-like protein 1 Synthetic RescuePMID:16710300
fbh1DNA helicase I Synthetic LethalityPMID:16135800
Synthetic LethalityPMID:16135799
cdc17ATP-dependent DNA replication ligase Cdc17 Synthetic LethalityPMID:9891047
tor1phosphatidylinositol kinase Tor1 Synthetic Growth DefectPMID:23703609
rad9checkpoint clamp complex protein Rad9 Phenotypic SuppressionPMID:9566891
pol1DNA polymerase alpha catalytic subunit Synthetic LethalityPMID:9891047
ufd1Cdc48-Ufd1-Npl4 complex subunit Ufd1 involved in ubiquitin-dependent protein catabolism (predicted) Synthetic Growth DefectPMID:24265825
wee1M phase inhibitor protein kinase Wee1 Synthetic Growth DefectPMID:1427071
pku80Ku domain protein Pku80 Phenotypic EnhancementPMID:19682091
mcm7MCM complex subunit Mcm7 Synthetic Growth DefectPMID:11606526
top1DNA topoisomerase I Synthetic RescuePMID:12084712
cdc20DNA polymerase epsilon catalytic subunit Pol2 Phenotypic EnhancementPMID:18667534
Synthetic LethalityPMID:9891047
ctf18RFC-like complex subunit Ctf18 Phenotypic EnhancementPMID:18045993
slx8SUMO-targeted ubiquitin-protein ligase E3 Slx8 Synthetic LethalityPMID:17762865
rad60DNA repair protein Rad60 Dosage LethalityPMID:16880212
Synthetic Lethality
Synthetic LethalityPMID:16354704
cdc13G2/M B-type cyclin Cdc13 Synthetic RescuePMID:12023299
rad55RecA family ATPase Rad55/Rhp55 Synthetic RescuePMID:16135800
Synthetic RescuePMID:15802523
Synthetic RescuePMID:14993467
crb2DNA repair protein Rad9 homolog, Rhp9 Synthetic Growth DefectPMID:12589755
Synthetic RescuePMID:12023299
Synthetic Growth DefectPMID:10373582
hrq1RecQ type DNA helicase Hrq1 (predicted) Synthetic Growth DefectPMID:22064477
Phenotypic Enhancement
orc1origin recognition complex subunit Orc1 Phenotypic EnhancementPMID:18667534
rad3ATR checkpoint kinase Rad3 Synthetic LethalityPMID:17178839
Synthetic Growth DefectPMID:17893680
hsk1Dbf4(Dfp1)-dependent protein kinase Hsk1 Synthetic LethalityPMID:11027263
cdc27DNA polymerase delta subunit Cdc27 Synthetic LethalityPMID:9891047
rad57RecA family ATPase Rad57/Rhp57 Synthetic RescuePMID:16135799
Phenotypic SuppressionPMID:23828040
Synthetic Growth Defect
Synthetic RescuePMID:14993467
Synthetic RescuePMID:16354704
Synthetic RescuePMID:23297345
mus81Holliday junction resolvase subunit Mus81 Synthetic LethalityPMID:16710300
Synthetic LethalityPMID:12084712
bub1mitotic spindle checkpoint kinase Bub1 Synthetic Growth DefectPMID:24469396
Phenotypic Suppression
rrp1ATP-dependent DNA helicase/ ubiquitin-protein ligase E3 (predicted) Synthetic Growth DefectPMID:23828040
mcl1DNA polymerase alpha accessory factor Mcl1 Synthetic LethalityPMID:12455694
Synthetic LethalityPMID:15643072
rad52DNA recombination protein Rad52 (previously Rad22) Synthetic Growth DefectPMID:11452021
Phenotypic EnhancementPMID:20705238
Synthetic LethalityPMID:10373582
cut8tethering factor for nuclear proteasome Cut8 Synthetic LethalityPMID:17178839
slx4structure-specific endonuclease subunit Slx4 Synthetic LethalityPMID:16710300
Synthetic LethalityPMID:14528010
chk1Chk1 protein kinase Phenotypic EnhancementPMID:9372918
Phenotypic SuppressionPMID:23584455
Phenotypic SuppressionPMID:21098121
Synthetic RescuePMID:24469396
Phenotypic Suppression
Phenotypic EnhancementPMID:15226425
srs2ATP-dependent DNA helicase, UvrD subfamily Synthetic Growth DefectPMID:17893680
Synthetic Growth DefectPMID:11452021
Synthetic Growth DefectPMID:16710300
Synthetic Growth DefectPMID:14993467
Synthetic Growth DefectPMID:12409469
rad2FEN-1 endonuclease Rad2 Phenotypic EnhancementPMID:7623848
Synthetic LethalityPMID:9891047
eme1Holliday junction resolvase subunit Eme1 Synthetic Growth DefectPMID:23584455
trt1telomerase reverse transcriptase 1 protein Trt1 Phenotypic EnhancementPMID:22313747
Synthetic Growth Defect
nse6Smc5-6 complex non-SMC subunit Nse6 Synthetic LethalityPMID:16478984
reb1RNA polymerase I transcription termination factor/ RNA polymerase II transcription factor Reb1 Synthetic Growth DefectPMID:16303848
swi1replication fork protection complex subunit Swi1 Synthetic Growth DefectPMID:16710300
Synthetic Growth DefectPMID:18045993
Synthetic Growth DefectPMID:20176980
rrp2ATP-dependent DNA helicase, ubiquitin-protein ligase E3 (predicted) Synthetic Growth DefectPMID:23828040
sfr1Swi five-dependent recombination repair protein Sfr1 Phenotypic SuppressionPMID:18769921
rad54DNA-dependent ATPase Rad54/Rhp54 Synthetic LethalityPMID:15802523
rad51RecA family recombinase Rad51/Rhp51 Synthetic RescuePMID:16135799
Synthetic RescuePMID:14993467
Synthetic RescuePMID:16135800
Synthetic RescuePMID:16354704
Synthetic LethalityPMID:15802523
Synthetic RescuePMID:23297345
Phenotypic Suppression
Synthetic Growth DefectPMID:20705238
Synthetic Growth DefectPMID:23828040
cdc23MCM-associated protein Mcm10 Synthetic LethalityPMID:9891047
uve1endonuclease Uve1 Phenotypic EnhancementPMID:9372918
Phenotypic EnhancementPMID:9092661
mus7DNA repair protein Mus7/Mms22 Phenotypic EnhancementPMID:17660542
Synthetic Growth DefectPMID:17307401
swi3replication fork protection complex subunit Swi3 Synthetic LethalityPMID:16710300
Synthetic LethalityPMID:18045993
Synthetic Growth DefectPMID:20176980
nse4Smc5-6 complex non-SMC subunit Nse4 Synthetic LethalityPMID:15485909
rad13DNA repair nuclease Rad13 Phenotypic EnhancementPMID:9372918
Phenotypic EnhancementPMID:9092661
Phenotypic EnhancementPMID:7623848
rad8ubiquitin-protein ligase E3 Rad8 Synthetic Growth DefectPMID:24875629
sws1SWIM domain containing-Srs2 interacting protein 1 Synthetic RescuePMID:16710300
External References
Database Identifier Description
NBRP SPAC2G11.12 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC2G11.12 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC2G11.12 BioGRID Interaction Datasets
Expression Viewer SPAC2G11.12 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC2G11.12 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC2G11.12 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC2G11.12 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC2G11.12 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC2G11.12 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC2G11.12 Cell Cycle Data
GEO SPAC2G11.12 GEO profiles
PInt SPAC2G11.12 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC2G11.12 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC2G11.12 Fission yeast phenotypic data & analysis
SPD / RIKEN39/39D09Orfeome Localization Data
UniProtKB/SwissProtQ09811ATP-dependent DNA helicase hus2/rqh1
ModBaseQ09811Database of comparative protein structure models
STRINGQ09811Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593092RecQ type DNA helicase Rqh1
RefSeq mRNANM_001018490972h- RecQ type DNA helicase Rqh1 (rqh1), mRNA
European Nucleotide ArchiveCAA70577.1ENA Protein Mapping
European Nucleotide ArchiveCAA91177.1ENA Protein Mapping
UniParcUPI000012CE81UniProt Archive

Literature for rqh1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014