rqh1 (SPAC2G11.12)


Gene Standard Namerqh1 Characterisation Statuspublished
Systematic IDSPAC2G11.12 Feature Typeprotein coding
Synonymshus2, rad12, rec9 Name DescriptionHydroxy Urea Sensitive
ProductRecQ type DNA helicase Rqh1 Product Size1328aa, 149.65 kDa
Genomic Location Chromosome I, 831918-836094 (4177nt); CDS:832027-836013 (3987nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding551
Annotation ExtensionEvidenceWith/FromReference
ATP-dependent 3'-5' DNA helicase activity12
Annotation ExtensionEvidenceWith/FromReference
DNA binding351
Annotation ExtensionEvidenceWith/FromReference
protein binding900
Annotation ExtensionEvidenceWith/FromReference
binds top3
GO Biological Process
Term NameCount
cellular response to DNA damage stimulus214
Annotation ExtensionEvidenceWith/FromReference
DNA duplex unwinding7
Annotation ExtensionEvidenceWith/FromReference
double-strand break repair via homologous recombination38
Annotation ExtensionEvidenceWith/FromReference
intra-S DNA damage checkpoint18
Annotation ExtensionEvidenceWith/FromReference
maintenance of rDNA7
Annotation ExtensionEvidenceWith/FromReference
mitotic recombination-dependent replication fork processing7
Annotation ExtensionEvidenceWith/FromReference
postreplication repair18
Annotation ExtensionEvidenceWith/FromReference
recombinational repair42
Annotation ExtensionEvidenceWith/FromReference
regulation of mitotic recombination involved in replication fork processing1
Annotation ExtensionEvidenceWith/FromReference
replication fork processing at rDNA locus3
Annotation ExtensionEvidenceWith/FromReference
resolution of mitotic recombination intermediates1
Annotation ExtensionEvidenceWith/FromReference
UV-damage excision repair17
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nuclear chromosome493
Annotation ExtensionEvidenceWith/FromReference
nuclear replication fork63
Annotation ExtensionEvidenceWith/FromReference
nucleolus366
Annotation ExtensionEvidenceWith/FromReference
RecQ helicase-Topo III complex3
Annotation ExtensionEvidenceWith/FromReference
site of double-strand break54
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased cell population growth on glucose carbon sourcerqh1Δ393
decreased spore germination frequencyrqh1Δ70
decreased vegetative cell population growthrqh1Δ745
normal growth during cellular response to gamma radiationrad12-502 (T543I)18
normal growth on camptothecinrqh1Δ44
normal growth on JM216rad12-502 (T543I)10
normal growth on tetraplatinrad12-502 (T543I)14
normal growth on triplatin tetranitraterad12-502 (T543I)11
sensitive to camptothecinrqh1Δ259
sensitive to cisplatinrad12-502 (T543I)111
rqh1Δ
sensitive to hydroxyurearad12-502 (T543I)595
hus2-22 (W789->stop)
rqh1Δ
sensitive to ionizing radiation during vegetative growthrad12-502 (T543I)76
rqh1Δ
sensitive to JM335rad12-502 (T543I)14
sensitive to methyl methanesulfonaterqh1Δ333
rec9-104
sensitive to oxaliplatinrad12-502 (T543I)16
sensitive to thiabendazolerqh1-hd (K547A)334
sensitive to UV during vegetative growthrqh1-K547R (K547R)161
rec9-104
rad12-502 (T543I)
rqh1-K547A (K547A)
hus2-22 (W789->stop)
rqh1Δ
slow vegetative cell population growthrad12-hd (helicase domain::ura4)369
rqh1Δ
K547I (K547I)
viable vegetative cell populationrqh1Δ3862

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic cell cyclerqh1Δ903
abnormal mitotic sister chromatid separationrqh1Δ53
abolished 5' deoxyribonuclease (pyrimidine dimer) activityrad12-502 (T543I)1
abolished ATP-dependent 3'-5' DNA helicase activityrad12-502 (T543I)1
rqh1-K547R (K547R)
rqh1-K547A (K547A)
abolished response to S-phase DNA damage checkpoint signalingrqh1Δ7
cell cycle arrest in mitotic G2 phase during response to DNA damagerqh1Δ2
cutrqh1Δ129
cut during cellular response to hydroxyureahus2-22 (W789->stop)44
rqh1Δ
cut during cellular response to UVrqh1Δ2
decreased double-strand break repair via homologous recombinationrqh1Δ13
decreased intergenic meiotic recombinationrqh1Δ34
decreased intragenic meiotic recombinationrqh1Δ37
affecting ade6rec9-104
decreased UV-damage excision repairrad12-502 (T543I)5
elongated vegetative cellrqh1Δ810
increased cellular reactive oxygen species level during vegetative growthrqh1Δ33
increased chromosomal translocationrqh1Δ2
increased double-strand break repair via nonhomologous end joiningrqh1Δ7
increased duration of mitotic M phase23
increased gross chromosomal rearrangement during replication fork processingrqh1Δ1
increased histone H2A phosphorylation at rDNA during vegetative growthrqh1Δ3
increased minichromosome loss during vegetative growthrqh1Δ81
increased mitotic recombinationrqh1Δ14
increased number of Rad52 focirqh1Δ55
increased number of Ssb1 focirqh1Δ7
inviable elongated vegetative cellrqh1Δ440
inviable mononucleate monoseptate vegetative cellrqh1Δ18
normal cell cycle regulation during cellular response to hydroxyurearqh1Δ20
normal frequency of meiotic crossover associated with gene conversionrqh1Δ2
normal mitotic cell cycle regulation during cellular response to UVrqh1Δ13
normal mitotic DNA damage checkpoint during cellular response to UVrqh1Δ9
normal protein kinase activity during mitotic S phase during cellular response to DNA damage3
affecting cds1rqh1Δ
normal protein level during vegetative growth103
affecting rqh1rad12-502 (T543I)
normal protein localization to telomere during vegetative growth14
affecting ssb1rqh1Δ
normal spatial extent of double-strand break processingrqh1Δ1
shortened telomeresrqh1Δ44
viable vegetative cellrqh1Δ3680
viable vegetative cell with normal cell shaperqh1Δ31

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
abolished cell population growth at high temperaturerqh1-hd (K547A), pot1Δ
decreased cell population growth on glucose carbon sourcerqh1Δ, trt1Δ
rqh1Δ, rad11-D223Y (D223Y)
rqh1-hd (K547A), rad11-D223Y (D223Y)
decreased spore germination frequencyrqh1Δ, fml1Δ, dmc1-12
rqh1Δ, dmc1-12
rqh1Δ, dmc1-12, rlp1-7, fml1Δ
sfr1-2, rqh1Δ
rlp1-7, rqh1Δ, fml1Δ
rlp1Δ, rqh1Δ, dmc1-12
rad55Δ, rqh1Δ, fml1Δ
rlp1-7, rqh1Δ
rad55Δ, rqh1Δ
rqh1Δ, fml1Δ
decreased vegetative cell population growthpab1-1 (nucleotides 673-684 duplicated; inserts amino acids TEVI after I228), rqh1Δ
rqh1Δ, cdc24ts (C114R)
dna2ts (L1097S), rqh1Δ
pot1Δ, rqh1-hd (K547A)
inviable cell populationrqh1Δ, nse3-R254E (R254E)
inviable vegetative cell populationrqh1-K457I (K457I), mcl1-1 (ntG372A)
rqh1Δ, mcl1-1 (ntG372A)
rqh1Δ, cut8Δ
top3Δ, rad3-ts, rqh1Δ
rad3-ts, rqh1Δ
rqh1Δ, rad52Δ
rad3Δ, rqh1Δ
mcl1+, rqh1Δ
pot1Δ, rqh1Δ
sensitive to bleomycinrqh1Δ, dna2ts (L1097S)
cdc24ts (C114R), rqh1Δ
sensitive to camptothecinrqh1Δ, dna2ts (L1097S)
cdc24ts (C114R), rqh1Δ
sensitive to hydroxyureapab1-1 (nucleotides 673-684 duplicated; inserts amino acids TEVI after I228), rqh1Δ
sensitive to ionizing radiation during vegetative growthrqh1Δ, cdc13-245 (E255R)
hta2-S128A (S128A), rqh1Δ, hta1-S129A (S129A)
rqh1Δ, rad51Δ
cdc13-245 (E255R), rqh1Δ, rad51Δ
rqh1Δ, crb2Δ
crb2-T215A (T215A), rqh1Δ
sensitive to methyl methanesulfonatepcf1Δ, rqh1Δ
spd1Δ, rqh1Δ, wdr70Δ
rqh1Δ, sfr1Δ
sensitive to thiabendazolerqh1-hd (K547A), trt1Δ
rqh1Δ, trt1Δ
rqh1-hd (K547A), pot1Δ
sensitive to UV during vegetative growthrqh1Δ, sfr1Δ
rad2-44, rad12-502 (T543I)
rqh1Δ, crb2Δ
rad13-A, rad12-502 (T543I)
rad22-67, rad12-502 (T543I)
slow vegetative cell population growthrqh1Δ, rad3-ts, rad51Δ
rad54Δ, rad3-ts, rqh1Δ
rad55Δ, rad3-ts, rqh1Δ
hta2-S128A (S128A), rqh1Δ, hta1-S129A (S129A)
rqh1Δ, cnd2-1
viable cell populationrqh1Δ, chk1Δ, cds1Δ
viable vegetative cell populationrad3-ts, cut8+, rqh1Δ
rad3-ts, rqh1Δ
top3Δ, rad12-hd (helicase domain::ura4)
rqh1Δ, cut8Δ
K547I (K547I), top3Δ
cut1+, rad3-ts, rqh1Δ

Cell Phenotype

Term NameGenotypes
abnormal double-strand break processingspd1Δ, rqh1Δ, wdr70Δ
rqh1Δ, spd1Δ
abnormal frequency of meiotic crossover associated with gene conversionrqh1Δ, dmc1-12
rlp1Δ, rqh1Δ, dmc1-12
abnormal mitotic recombinationrqh1-hd (K547A), pot1Δ
circularized chromosomerqh1-hd (K547A), pot1Δ, rad51Δ
rqh1-hd (K547A), rad51Δ, trt1Δ
cutrad3-ts, rqh1Δ
rqh1-hd (K547A), pot1Δ
decreased histone H2B-K119 ubiquitination at double-strand break site during vegetative growthrqh1Δ, exo1Δ, spd1Δ
decreased intergenic meiotic recombinationrqh1Δ, dmc1-12, rlp1-7, fml1Δ
rlp1Δ, rqh1Δ, dmc1-12
sfr1-2, rqh1Δ
rad55Δ, rqh1Δ, fml1Δ
rqh1Δ, fml1Δ
rlp1-7, rqh1Δ
rad55Δ, rqh1Δ
rqh1Δ, dmc1-12
rqh1Δ, fml1Δ, dmc1-12
sfr1-2, rqh1Δ, fml1Δ
rlp1-7, rqh1Δ, fml1Δ
decreased intragenic meiotic recombinationsfr1-2, rqh1Δ
rad55Δ, rqh1Δ
sfr1-2, rqh1Δ, fml1Δ
rqh1Δ, dmc1-12
rqh1Δ, dmc1-12, rlp1-7, fml1Δ
rlp1-7, rqh1Δ, fml1Δ
rqh1Δ, fml1Δ, dmc1-12
rad55Δ, rqh1Δ, fml1Δ
rlp1Δ, rqh1Δ, dmc1-12
rqh1Δ, fml1Δ
rlp1-7, rqh1Δ
decreased number of Rad51 foci during cellular response to ionizing radiationrqh1Δ, rad51Δ
decreased protein localization to double-strand break site
affects localization of wdr70rqh1Δ, exo1Δ, spd1Δ
decreased punctate nuclear protein localization during cellular response to ionizing radiation
affects localization of top3rqh1Δ, cdc13-245 (E255R)
decreased replication fork arrest at mating-type locusrtf2Δ, rqh1Δ
decreased site-specific DNA replication termination at RTS1 barrierrtf2Δ, rqh1Δ
decreased spatial extent of double-strand break processingrqh1Δ, cdc24ts (C114R)
elongated telomeresrqh1Δ, rad11-D223Y (D223Y), taz1Δ
rqh1Δ, taz1Δ
rqh1-K547A (K547A), rad11-D223Y (D223Y), taz1Δ
rqh1-K547R (K547R), rad11-D223Y (D223Y), taz1Δ
elongated vegetative cellrqh1-hd (K547A), pot1Δ
entangled telomeresrqh1-hd (K547A), pot1Δ
rqh1-hd (K547A), trt1Δ
increased frequency of meiotic crossover associated with gene conversionrqh1Δ, dmc1-12, rlp1-7, fml1Δ
rlp1-7, rqh1Δ, fml1Δ
rlp1-7, rqh1Δ
sfr1-2, rqh1Δ, fml1Δ
rqh1Δ, fml1Δ, dmc1-12
sfr1-2, rqh1Δ
rad55Δ, rqh1Δ
rqh1Δ, fml1Δ
rad55Δ, rqh1Δ, fml1Δ
increased number of Rad52 focirqh1Δ, pku80Δ
rqh1Δ, brc1Δ
increased number of Rad52 foci during vegetative growthrad3-ts, rqh1Δ
increased number of Ssb1 focirqh1Δ, pku80Δ
rqh1-hd (K547A), trt1Δ
rqh1-hd (K547A), pot1Δ
inviable after spore germination with elongated germ tube and fragmented nucleusrqh1Δ, cut8Δ
loss of gross chromosomal rearrangement during replication fork processingpcf1Δ, rqh1Δ
mitotic catastrophe with cut, small cellhus2-22 (W789->stop), wee1-50
normal DNA recombination frequency during cellular response to ionizing radiationrqh1Δ, cdc13-245 (E255R)
crb2-T215A (T215A), rqh1Δ
normal protein localization to telomere during vegetative growth
affecting ssb1rqh1Δ, rad11-D223Y (D223Y)
progressively shortening telomeresrqh1Δ, trt1Δ
shortened telomeresrqh1-hd (K547A), trt1Δ
telomeric regions absent from linear chromosomesrqh1Δ, trt1Δ, taz1Δ
Target Of
OntologyRelationshipGeneProduct
FYPO localization affected by mutation in cdc13 G2/M B-type cyclin Cdc13
FYPO localization affected by mutation in ssb1 DNA replication factor A subunit Ssb1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons831918..836094
mRNA831918..836094
5' UTR831918..832026PMID:21511999
CDS832027..836013
3' UTR836014..836094PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00270 Pfam IPR011545 DEAD/DEAH box helicase domain 522 695 42
PF09382 Pfam IPR018982 RQC domain 921 1039 1
PF00271 Pfam IPR001650 Helicase, C-terminal 731 837 67
PF16124 Pfam IPR032284 ATP-dependent DNA helicase RecQ, zinc-binding domain 849 914 1
PF00570 Pfam IPR002121 HRDC domain 1120 1185 2
SM00487 SMART IPR014001 Helicase superfamily 1/2, ATP-binding domain 516 723 69
SM00341 SMART IPR002121 HRDC domain 1115 1195 2
SM00490 SMART IPR001650 Helicase, C-terminal 756 838 68
SM00956 SMART IPR018982 RQC domain 924 1035 1
PS00690 Prosite Patterns IPR002464 DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site 646 655 10
PS51192 Prosite Profiles IPR014001 Helicase superfamily 1/2, ATP-binding domain 528 707 67
PS50967 Prosite Profiles IPR002121 HRDC domain 1115 1195 2
PS51194 Prosite Profiles IPR001650 Helicase, C-terminal 728 876 67
PTHR13710 HMMPANTHER 480 1319 4
PTHR13710:SF70 HMMPANTHER 480 1319 1
PTHR13710:SF70 HMMPANTHER 7 205 1
PTHR13710 HMMPANTHER 7 205 4
1.10.10.10 Gene3D IPR011991 Winged helix-turn-helix DNA-binding domain 851 1034 46
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 500 711 288
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 712 850 288
1.10.150.80 Gene3D IPR002121 HRDC domain 1118 1194 1
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 812 854 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 495 670 294
SSF47819 SuperFamily IPR010997 HRDC-like 1120 1196 4
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 713 967 294
Coil ncoils Predicted coiled-coil protein (DUF2205) 866 886 1049
TIGR00614 TIGRFAM IPR004589 DNA helicase, ATP-dependent, RecQ type 510 969 1

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000174DEAD/DEAH box helicaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000174
PBO:0000619RecQ familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000619
PBO:0011078Req family helicaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0011078

Protein Properties

Ave. residue weight 112.69 Da
Charge 11.00
Codon Adaptation Index 0.40
Isoelectric point 7.17
Molecular weight 149.65 kDa
Number of residues 1328
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS1138 2293
Annotation ExtensionEvidenceResidueReference
IDA S1138 PMID:25720772
mannosylated residue 89
Annotation ExtensionEvidenceResidueReference
mass spectrometry evidence PMID:26644575
O-phosphorylated residueS104,S106 2461
Annotation ExtensionEvidenceResidueReference
IDA S104,S106 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for rqh1 (SPAC2G11.12)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.5during GO:0072690PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.47during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by E. coli RusApartial PMID:10835372
functionally complemented by human GEN1partial PMID:20040574
Disease Association
DescriptionQualifierReferenceCount
cancer148
Bloom's syndrome1
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3087
conserved in fungi4608
conserved in eukaryotes4516
conserved in bacteria1005
conserved in metazoa3498
conserved in vertebrates3473
conserved in archaea243
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC2G11.12 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bypfh15' to 3' DNA helicase, involved in DNA recombination and repair Pif1 Affinity Capture-MSPMID:27611590
affinity captured bypcf1CAF assembly factor (CAF-1) complex large subunit Pcf1 Affinity Capture-WesternPMID:25313826
affinity captured bytop3DNA topoisomerase III Affinity Capture-WesternPMID:12724426
affinity captured bycdc2cyclin-dependent protein kinase Cdk1/Cdc2 Affinity Capture-WesternPMID:24861625
binds activation domain construct withrqh1RecQ type DNA helicase Rqh1 Two-hybridPMID:26771498
binds activation domain construct withatg11autophagy associated protein Atg11 Two-hybridPMID:26771498
binds activation domain construct withSPCP20C8.01cmug2/mug135/meu2 family Two-hybridPMID:26771498
binds activation domain construct withcdc23MCM-associated protein Mcm10 Two-hybridPMID:26771498
binds activation domain construct withSPBC337.02cmug2/mug135/meu2 family Two-hybridPMID:26771498
binds activation domain construct withspo7sporulation protein Spo7 Two-hybridPMID:26771498
binds activation domain construct withcbh1kinetochore protein, CENP-B homolog Cbh1 Two-hybridPMID:26771498
binds activation domain construct withssb1DNA replication factor A subunit Ssb1 Two-hybridPMID:26771498
binds activation domain construct withSPCC569.01cmug2/mug135/meu2 family Two-hybridPMID:26771498
binds activation domain construct withusp104U1 snRNP-associated protein Usp104 Two-hybridPMID:26771498
modified bypli1SUMO E3 ligase Pli1 Biochemical ActivityPMID:18031226
modified bynse2Smc5-6 complex non-SMC subunit SUMO ligase subunit Nse2 Biochemical ActivityPMID:18031226
affinity capturestop3DNA topoisomerase III Affinity Capture-WesternPMID:12724426
affinity capturesssb1DNA replication factor A subunit Ssb1 Affinity Capture-WesternPMID:17429064
affinity capturestop1DNA topoisomerase I Affinity Capture-WesternPMID:15702347
binds DNA-binding domain construct withrmi1RecQ mediated genome instability protein Rmi1 (predicted) Two-hybridPMID:26771498
binds DNA-binding domain construct withrcl1rRNA processing protein Rcl1 (predicted) Two-hybridPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC2G11.12 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
enhances phenotype ofsfr1Swi five-dependent recombination mediator Sfr1 Phenotypic EnhancementPMID:25414342
enhances phenotype offml1ATP-dependent 3' to 5' DNA helicase, FANCM ortholog Fml1 Phenotypic EnhancementPMID:25414342
enhances phenotype ofhrq1RecQ type DNA helicase Hrq1 (predicted) Phenotypic EnhancementPMID:22064477
enhances phenotype ofdmc1RecA family ATPase Dmc1 Phenotypic EnhancementPMID:25414342
enhances phenotype ofexo1exonuclease I Exo1 Phenotypic EnhancementPMID:21931565
enhances phenotype ofcdc20DNA polymerase epsilon catalytic subunit Pol2 Phenotypic EnhancementPMID:18667534
enhances phenotype ofpku80Ku domain protein Pku80 Phenotypic EnhancementPMID:19682091
enhances phenotype ofrad2FEN-1 endonuclease Rad2 Phenotypic EnhancementPMID:7623848
enhances phenotype ofpot1telomere end-binding protein Pot1 Phenotypic EnhancementPMID:18722173
enhances phenotype oforc1origin recognition complex subunit Orc1 Phenotypic EnhancementPMID:18667534
enhances phenotype ofuve1endonuclease Uve1 Phenotypic EnhancementPMID:9092661
enhances phenotype ofctf18RFC-like complex subunit Ctf18 Phenotypic EnhancementPMID:18045993
enhances phenotype ofrad52DNA recombination protein, Rad51 mediator Rad52 (previously Rad22) Phenotypic EnhancementPMID:20705238
enhances phenotype ofmus7DNA repair protein Mus7/Mms22 Phenotypic EnhancementPMID:17660542
synthetic growth defect withcrb2DNA repair protein Rad9 homolog Crb2 Synthetic Growth DefectPMID:10373582
synthetic growth defect withcnd2condensin complex non-SMC subunit Cnd2 Synthetic Growth DefectPMID:12000964
synthetic growth defect withwdr70DDB1-CUL4-associated factor (DCAF), Wdr70 Synthetic Growth DefectPMID:27098497
synthetic growth defect withpab1protein phosphatase PP2A regulatory subunit B-55 Pab1 Synthetic Growth DefectPMID:27398807
synthetic growth defect withssb1DNA replication factor A subunit Ssb1 Synthetic Growth DefectPMID:26041456
synthetic growth defect withmms1E3 ubiquitin ligase complex subunit Mms1 (predicted) Synthetic Growth DefectPMID:19819763
synthetic growth defect withbrc1BRCT domain protein Brc1 Synthetic Growth DefectPMID:15972456
synthetic growth defect withswi2Swi5 complex subunit Swi2 Synthetic Growth DefectPMID:18045993
synthetic growth defect withtrt1telomerase reverse transcriptase 1 protein Trt1 Synthetic Growth DefectPMID:22313747
synthetic growth defect withrad3ATR checkpoint kinase Rad3 Synthetic Growth DefectPMID:17893680
synthetic growth defect withswi1replication fork protection complex subunit Swi1 Synthetic Growth DefectPMID:20176980
synthetic growth defect withrrp2ATP-dependent DNA helicase, ubiquitin-protein ligase E3 (predicted) Synthetic Growth DefectPMID:23828040
synthetic growth defect withrad57RecA family ATPase Rad57/Rhp57 Synthetic Growth DefectPMID:23828040
synthetic growth defect withufd1Hrd1 ubiquitin ligase complex subunit Ufd1 (predicted) Synthetic Growth DefectPMID:24265825
synthetic growth defect withsrs2ATP-dependent DNA helicase, UvrD subfamily Synthetic Growth DefectPMID:14993467
synthetic growth defect witheme1Holliday junction resolvase subunit Eme1 Synthetic Growth DefectPMID:23584455
synthetic growth defect withswi3replication fork protection complex subunit Swi3 Synthetic Growth DefectPMID:20176980
synthetic growth defect withtop3DNA topoisomerase III Synthetic Growth DefectPMID:15702347
synthetic growth defect withpot1telomere end-binding protein Pot1 Synthetic Growth DefectPMID:21098121
synthetic growth defect withhta2histone H2A beta Synthetic Growth DefectPMID:15226425
synthetic growth defect withhrq1RecQ type DNA helicase Hrq1 (predicted) Synthetic Growth DefectPMID:22064477
synthetic growth defect withpcf1CAF assembly factor (CAF-1) complex large subunit Pcf1 Synthetic Growth DefectPMID:25313826
synthetic growth defect withhta1histone H2A alpha Synthetic Growth DefectPMID:20661445
synthetic growth defect withtor1serine/threonine protein kinase Tor1 Synthetic Growth DefectPMID:23703609
synthetic growth defect withrad52DNA recombination protein, Rad51 mediator Rad52 (previously Rad22) Synthetic Growth DefectPMID:11452021
synthetic growth defect withrad8ubiquitin-protein ligase E3 involved in DNA repair Rad8 Synthetic Growth DefectPMID:24875629
synthetic growth defect withrhp18Rad18 homolog ubiquitin protein ligase E3, Rhp18 Synthetic Growth DefectPMID:11523791
synthetic growth defect withbub1mitotic spindle checkpoint kinase Bub1 Synthetic Growth DefectPMID:24469396
synthetic growth defect withrad51RecA family recombinase Rad51/Rhp51 Synthetic Growth DefectPMID:23828040
synthetic growth defect withrrp1ATP-dependent DNA helicase/ ubiquitin-protein ligase E3 (predicted) Synthetic Growth DefectPMID:23828040
synthetic growth defect withmcm7MCM complex subunit Mcm7 Synthetic Growth DefectPMID:11606526
synthetic growth defect withmad2mitotic spindle checkpoint protein Mad2 Synthetic Growth DefectPMID:16303848
synthetic growth defect withreb1RNA polymerase I transcription termination factor/ RNA polymerase II transcription factor Reb1 Synthetic Growth DefectPMID:16303848
synthetic growth defect withmus7DNA repair protein Mus7/Mms22 Synthetic Growth DefectPMID:17307401
synthetically rescued bydmc1RecA family ATPase Dmc1 Synthetic RescuePMID:19037101
synthetically rescued byswi2Swi5 complex subunit Swi2 Synthetic RescuePMID:19037101
synthetically rescued byswi5Swi5 protein Synthetic RescuePMID:19037101
synthetically rescued bypcf2CAF assembly factor (CAF-1) complex subunit B, Pcf2 Synthetic RescuePMID:25313826
synthetically rescued byrad57RecA family ATPase Rad57/Rhp57 Synthetic RescuePMID:23297345
synthetically rescued byrdl1RAD51D-like protein 1 Synthetic RescuePMID:16710300
synthetically rescued byrad51RecA family recombinase Rad51/Rhp51 Synthetic RescuePMID:16135800
synthetically rescued byrad55RecA family ATPase Rad55/Rhp55 Synthetic RescuePMID:15802523
synthetically rescued bypcf1CAF assembly factor (CAF-1) complex large subunit Pcf1 Synthetic RescuePMID:25313826
synthetically rescued bychk1Chk1 protein kinase Synthetic RescuePMID:26093291
synthetically rescued bymik1mitotic inhibitor kinase Mik1 Synthetic RescuePMID:26093291
synthetically rescued bycdc2cyclin-dependent protein kinase Cdk1/Cdc2 Synthetic RescuePMID:26093291
synthetically rescued bysws1SWIM domain containing-Srs2 interacting protein 1 Synthetic RescuePMID:16710300
synthetically rescued bywee1M phase inhibitor protein kinase Wee1 Synthetic RescuePMID:26093291
synthetically rescued bytop1DNA topoisomerase I Synthetic RescuePMID:12084712
synthetically rescued byexo1exonuclease I Exo1 Synthetic RescuePMID:23297345
synthetic lethal withpol1DNA polymerase alpha catalytic subunit Synthetic LethalityPMID:9693370
synthetic lethal withrad52DNA recombination protein, Rad51 mediator Rad52 (previously Rad22) Synthetic LethalityPMID:10373582
synthetic lethal withswi1replication fork protection complex subunit Swi1 Synthetic LethalityPMID:19037101
synthetic lethal withwee1M phase inhibitor protein kinase Wee1 Synthetic LethalityPMID:1427071
synthetic lethal withslx1structure-specific endonuclease catalytic subunit Slx1 Synthetic LethalityPMID:14528010
synthetic lethal withrad16DNA repair endonuclease XPF Synthetic LethalityPMID:25293972
synthetic lethal withfbh1DNA helicase I, ubiquitin ligase F-box adaptor Fbh1 Synthetic LethalityPMID:16135799
synthetic lethal withcdc6DNA polymerase delta catalytic subunit Cdc6 Synthetic LethalityPMID:9891047
synthetic lethal withnse6Smc5-6 complex non-SMC subunit Nse6 Synthetic LethalityPMID:16478984
synthetic lethal withswi3replication fork protection complex subunit Swi3 Synthetic LethalityPMID:16710300
synthetic lethal withcut8tethering factor for nuclear proteasome Cut8 Synthetic LethalityPMID:17178839
synthetic lethal withpot1telomere end-binding protein Pot1 Synthetic LethalityPMID:21098121
synthetic lethal withrad2FEN-1 endonuclease Rad2 Synthetic LethalityPMID:9891047
synthetic lethal withslx4structure-specific endonuclease subunit Slx4 Synthetic LethalityPMID:16710300
synthetic lethal withpcf1CAF assembly factor (CAF-1) complex large subunit Pcf1 Synthetic LethalityPMID:25313826
synthetic lethal withmcm4MCM complex subunit Mcm4/Cdc21 Synthetic LethalityPMID:11606526
synthetic lethal withmcl1DNA polymerase alpha accessory factor Mcl1 Synthetic LethalityPMID:15643072
synthetic lethal withcdc20DNA polymerase epsilon catalytic subunit Pol2 Synthetic LethalityPMID:9891047
synthetic lethal withmus81Holliday junction resolvase subunit Mus81 Synthetic LethalityPMID:12084712
synthetic lethal withrad54DNA-dependent ATPase Rad54/Rhp54 Synthetic LethalityPMID:15802523
synthetic lethal withnse3Smc5-6 complex non-SMC MAGE family subunit Nse3 Synthetic LethalityPMID:26446992
synthetic lethal withrad60DNA repair protein, SUMO-related Rad60 Synthetic LethalityPMID:16354704
synthetic lethal withnse4Smc5-6 complex non-SMC delta-kleisin subunit Nse4 Synthetic LethalityPMID:15485909
synthetic lethal withrad3ATR checkpoint kinase Rad3 Synthetic LethalityPMID:17178839
synthetic lethal withslx8SUMO-targeted ubiquitin-protein ligase E3 Slx8 Synthetic LethalityPMID:17762865
synthetic lethal withcdc23MCM-associated protein Mcm10 Synthetic LethalityPMID:9891047
synthetic lethal withcdc17ATP-dependent DNA replication ligase Cdc17 Synthetic LethalityPMID:9891047
synthetic lethal withrad51RecA family recombinase Rad51/Rhp51 Synthetic LethalityPMID:15802523
synthetic lethal withcdc1DNA polymerase delta small subunit Cdc1 Synthetic LethalityPMID:9891047
synthetic lethal withhsk1Dbf4(Dfp1)-dependent protein kinase Hsk1 Synthetic LethalityPMID:11027263
synthetic lethal withcdc27DNA polymerase delta subunit Cdc27 Synthetic LethalityPMID:9891047
rescued byrad57RecA family ATPase Rad57/Rhp57 Phenotypic SuppressionPMID:23828040
rescued byrad9checkpoint clamp complex protein Rad9 Phenotypic SuppressionPMID:9566891
rescued bypcf1CAF assembly factor (CAF-1) complex large subunit Pcf1 Phenotypic SuppressionPMID:25313826
rescued bychk1Chk1 protein kinase Phenotypic SuppressionPMID:23584455
rescued bymad2mitotic spindle checkpoint protein Mad2 Phenotypic SuppressionPMID:24469396
rescued byswi5Swi5 protein Phenotypic SuppressionPMID:23828040
rescued bysfr1Swi five-dependent recombination mediator Sfr1 Phenotypic SuppressionPMID:18769921
rescued byrad51RecA family recombinase Rad51/Rhp51 Phenotypic SuppressionPMID:23297345
rescued bybub1mitotic spindle checkpoint kinase Bub1 Phenotypic SuppressionPMID:24469396
synthetically rescuescdc13G2/M B-type cyclin Cdc13 Synthetic RescuePMID:12023299
synthetically rescuestop3DNA topoisomerase III Synthetic RescuePMID:23516381
synthetically rescuescrb2DNA repair protein Rad9 homolog Crb2 Synthetic RescuePMID:12023299
synthetically rescuespot1telomere end-binding protein Pot1 Synthetic RescuePMID:26465752
phenotype enhanced byrad13DNA repair nuclease Rad13 Phenotypic EnhancementPMID:9372918
phenotype enhanced bychk1Chk1 protein kinase Phenotypic EnhancementPMID:15226425
phenotype enhanced byuve1endonuclease Uve1 Phenotypic EnhancementPMID:9372918
phenotype enhanced bytrt1telomerase reverse transcriptase 1 protein Trt1 Phenotypic EnhancementPMID:22313747
rescuespcf1CAF assembly factor (CAF-1) complex large subunit Pcf1 Phenotypic SuppressionPMID:25313826
rescuespot1telomere end-binding protein Pot1 Phenotypic SuppressionPMID:21098121
rescuespcf2CAF assembly factor (CAF-1) complex subunit B, Pcf2 Phenotypic SuppressionPMID:25313826
inviable in presence of overexpressedrad60DNA repair protein, SUMO-related Rad60 Dosage LethalityPMID:16880212
External References
Database Identifier Description
NBRP SPAC2G11.12 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC2G11.12 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC2G11.12 BioGRID Interaction Datasets
Expression Viewer SPAC2G11.12 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC2G11.12 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC2G11.12 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC2G11.12 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC2G11.12 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC2G11.12 Transcriptome Viewer (Bähler Lab)
GEO SPAC2G11.12 GEO profiles
PInt SPAC2G11.12 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC2G11.12 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC2G11.12 Fission yeast phenotypic data & analysis
Cyclebase SPAC2G11.12.1 Cell Cycle Data
SPD / RIKEN39/39D09Orfeome Localization Data
UniProtKB/SwissProtQ09811ATP-dependent DNA helicase hus2/rqh1
ModBaseQ09811Database of comparative protein structure models
STRINGQ09811Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593092RecQ type DNA helicase Rqh1
RefSeq mRNANM_001018490972h- RecQ type DNA helicase Rqh1 (rqh1), mRNA
European Nucleotide ArchiveCAA70577.1ENA Protein Mapping
European Nucleotide ArchiveCAA91177.1ENA Protein Mapping
UniParcUPI000012CE81UniProt Archive

Literature for rqh1

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Release Version: PomBase:30_62 - 30 Jan 2017