atg22 (SPAC2G11.13)


Gene Standard Nameatg22 Characterisation Statuspublished
Systematic IDSPAC2G11.13 Feature Typeprotein coding
Synonyms Name Description
Productvacuolar amino acid efflux transporter Atg22 Product Size529aa, 58.64 kDa
Genomic Location Chromosome I, 836206-838256 (2051nt); CDS:836383-837972 (1590nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0034639L-amino acid efflux transmembrane transporter activityISOSGD:S000000543GO_REF:00000242
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:1902475L-alpha-amino acid transmembrane transportISOSGD:S000000543GO_REF:00000249
GO:0032974amino acid transmembrane export from vacuoleISOSGD:S000000543GO_REF:00000242
NOT GO:0006914autophagyIMPPMID:19778961
GO:0006995cellular response to nitrogen starvationIMPPMID:1977896193
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000329fungal-type vacuole membraneIDAPMID:1682337291
GO:0071627integral component of fungal-type vacuolar membraneISOSGD:S000000543GO_REF:00000246
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000085sensitive to camptothecincompetitive growth assay evidenceatg22ΔNullPMID:20537132200
FYPO:0002060viable vegetative cell populationMicroscopyatg22ΔNullPECO:0000005, PECO:0000137PMID:236978063755
Microscopyatg22ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopyatg22ΔNullPECO:0000005, PECO:0000137PMID:236978063078
penetrance FYPO_EXT:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1836206838256

UTRs

Region Start End Reference
five_prime_UTR836206836382PMID:21511999
three_prime_UTR837973838256PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF11700 Pfam IPR024671 Autophagy-related protein 22-like 13 519 1
TMhelix TMHMM 397 419 959
TMhelix TMHMM 21 43 959
TMhelix TMHMM 116 135 959
TMhelix TMHMM 431 450 959
TMhelix TMHMM 240 262 959
TMhelix TMHMM 268 285 959
TMhelix TMHMM 465 487 959
TMhelix TMHMM 139 161 959
TMhelix TMHMM 85 107 959
TMhelix TMHMM 494 516 959
TMhelix TMHMM 365 387 959
SSF103473 SuperFamily IPR016196 239 521 76
Low complexity (SEG) seg 436 444
Low complexity (SEG) seg 82 93
Low complexity (SEG) seg 120 138

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 110.85 Da
Charge 2.00
Isoelectric point 6.74
Molecular weight 58.64 kDa
Number of residues 529
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1760.07during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
10755.69during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.8during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi only603
conserved in fungi4564
conserved in eukaryotes4482
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
cdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
ddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
slx4structure-specific endonuclease subunit Slx4 Negative GeneticPMID:22681890
pob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
par1protein phosphatase regulatory subunit Par1 Positive GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
cid12poly(A) polymerase Cid12 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC2G11.13 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC2G11.13 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC2G11.13 BioGRID Interaction Datasets
Expression Viewer SPAC2G11.13 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC2G11.13 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC2G11.13 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC2G11.13 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC2G11.13 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC2G11.13 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC2G11.13 Cell Cycle Data
GEO SPAC2G11.13 GEO profiles
PInt SPAC2G11.13 Protein-Protein Interaction Predictor (Bähler Lab)
SPD / RIKEN25/25F02Orfeome Localization Data
WikiGene2541826vacuolar amino acid efflux transporter Atg22 (predicted)
EntrezGene2541826vacuolar amino acid efflux transporter Atg22 (predicted)
UniProtKB/SwissProtQ09812Autophagy-related protein 22
ModBaseQ09812Database of comparative protein structure models
StringQ09812Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593093vacuolar amino acid efflux transporter Atg22 (predicted)
RefSeq mRNANM_001018491972h- vacuolar amino acid efflux transporter Atg22 (predicted) (atg22), mRNA
European Nucleotide ArchiveCAA91178ENA Protein Mapping
UniParcUPI000013A00BUniProt Archive

Literature for atg22

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014