atg22 (SPAC2G11.13)

Gene Standard Nameatg22 Characterisation Statuspublished
Systematic IDSPAC2G11.13 Feature Typeprotein coding
Synonyms Name Description
Productvacuolar amino acid transmembrane transporter Atg22 Product Size529aa, 58.64 kDa
Genomic Location Chromosome I, 836206-838256 (2051nt); CDS:836383-837972 (1590nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
L-amino acid efflux transmembrane transporter activity1
Annotation ExtensionEvidenceWith/FromReference
L-amino acid transmembrane transporter activity9
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
amino acid transmembrane export from vacuole2
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
L-alpha-amino acid transmembrane transport11
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
fungal-type vacuole membrane91
Annotation ExtensionEvidenceWith/FromReference
integral component of fungal-type vacuolar membrane6
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Single-allele Phenotypes

Population Phenotype

Term NameGenotypesCount
sensitive to camptothecinatg22Δ225
viable vegetative cell populationatg22Δ3815

Cell Phenotype

Term NameGenotypesCount
decreased cellular arginine levelatg22Δ5
decreased cellular aspartate levelatg22Δ1
decreased cellular glutamate levelatg22Δ1
decreased cellular histidine levelatg22Δ5
decreased cellular isoleucine levelatg22Δ4
decreased cellular lysine levelatg22Δ5
decreased cellular ornithine levelatg22Δ1
decreased cellular threonine levelatg22Δ3
decreased cellular valine levelatg22Δ2
decreased sporulationatg22Δ89
decreased vacuolar arginine levelatg22Δ3
decreased vacuolar aspartate levelatg22Δ1
decreased vacuolar glutamate levelatg22Δ1
decreased vacuolar histidine levelatg22Δ3
decreased vacuolar lysine levelatg22Δ3
decreased vacuolar ornithine levelatg22Δ1
normal cellular alanine levelatg22Δ2
normal cellular glutamine levelatg22Δ1
normal cellular glycine levelatg22Δ4
normal cellular leucine levelatg22Δ4
normal cellular methionine levelatg22Δ2
normal cellular phenylalanine levelatg22Δ2
normal cellular serine levelatg22Δ2
normal cellular tryptophan levelatg22Δ2
normal cellular tyrosine levelatg22Δ2
normal vacuolar alanine levelatg22Δ2
normal vacuolar asparagine levelatg22Δ2
normal vacuolar glutamine levelatg22Δ1
normal vacuolar glycine levelatg22Δ2
normal vacuolar isoleucine levelatg22Δ2
normal vacuolar leucine levelatg22Δ2
normal vacuolar methionine levelatg22Δ2
normal vacuolar phenylalanine levelatg22Δ2
normal vacuolar serine levelatg22Δ2
normal vacuolar threonine levelatg22Δ2
normal vacuolar tryptophan levelatg22Δ2
normal vacuolar tyrosine levelatg22Δ1
normal vacuolar valine levelatg22Δ2
viable vegetative cell with normal cell morphologyatg22Δ3095
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR836206..836382PMID:21511999
3' UTR837973..838256PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF11700 Pfam IPR024671 Autophagy-related protein 22-like 13 518 1
TMhelix TMHMM 431 450 959
TMhelix TMHMM 240 262 959
TMhelix TMHMM 365 387 959
TMhelix TMHMM 494 516 959
TMhelix TMHMM 85 107 959
TMhelix TMHMM 116 135 959
TMhelix TMHMM 465 487 959
TMhelix TMHMM 397 419 959
TMhelix TMHMM 139 161 959
TMhelix TMHMM 21 43 959
TMhelix TMHMM 268 285 959
PTHR11360 HMMPANTHER 154 526 1
PTHR11360:SF97 HMMPANTHER 154 526 1
1.20.1250.20 Gene3D Glycerate/sugar phosphate transporter, conserved site 332 516 72
SSF103473 SuperFamily IPR020846 Major facilitator superfamily domain 239 521 76

View domain organization at Pfam

Term IDTerm NameReferenceCount

Protein Properties

Ave. residue weight 110.85 Da
Charge 2.00
Codon Adpatation Index 0.40
Isoelectric point 6.74
Molecular weight 58.64 kDa
Number of residues 529

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS527
S3, S529
present during mitotic M phaseS3
present during mitotic M phaseS529
Annotation ExtensionEvidenceResidueReference
experimental evidence S3 PMID:24763107
present during mitotic M phase experimental evidence S3 PMID:21712547
present during mitotic M phase experimental evidence S527 PMID:21712547
experimental evidence S529 PMID:24763107
present during mitotic M phase experimental evidence S529 PMID:21712547
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1760.07during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
10755.69during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.8during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
0.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
predominantly single copy (one to one)3090
conserved in fungi only620
conserved in fungi4604
conserved in eukaryotes4514

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withcdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
positive genetic interaction withcid12poly(A) polymerase Cid12 Positive GeneticPMID:22681890
negative genetic interaction withddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
negative genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
positive genetic interaction withpar1protein phosphatase regulatory subunit Par1 Positive GeneticPMID:22681890
negative genetic interaction withpob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
negative genetic interaction withslx4structure-specific endonuclease subunit Slx4 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC2G11.13 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC2G11.13 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC2G11.13 BioGRID Interaction Datasets
Expression Viewer SPAC2G11.13 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC2G11.13 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC2G11.13 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC2G11.13 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC2G11.13 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC2G11.13 Transcriptome Viewer (Bähler Lab)
GEO SPAC2G11.13 GEO profiles
PInt SPAC2G11.13 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC2G11.13 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC2G11.13 Fission yeast phenotypic data & analysis
Cyclebase SPAC2G11.13.1 Cell Cycle Data
SPD / RIKEN25/25F02Orfeome Localization Data
UniProtKB/SwissProtQ09812Autophagy-related protein 22
ModBaseQ09812Database of comparative protein structure models
STRINGQ09812Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593093vacuolar amino acid efflux transporter Atg22
RefSeq mRNANM_001018491972h- vacuolar amino acid efflux transporter Atg22 (atg22), mRNA
European Nucleotide ArchiveCU329670ENA EMBL mapping
European Nucleotide ArchiveCAA91178ENA Protein Mapping
European Nucleotide ArchiveCAA91178.1ENA Protein Mapping
UniParcUPI000013A00BUniProt Archive

Literature for atg22

Search: Europe PMC or PubMed

Release Version: PomBase:28_55 - 09 Sep 2015