atg22 (SPAC2G11.13)


Gene Standard Nameatg22 Characterisation Statuspublished
Systematic IDSPAC2G11.13 Feature Typeprotein coding
Synonyms Name Description
Productvacuolar amino acid efflux transporter Atg22 Product Size529aa, 58.64 kDa
Genomic Location Chromosome I, 836206-838256 (2051nt); CDS:836383-837972 (1590nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
L-amino acid efflux transmembrane transporter activity2
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
amino acid transmembrane export from vacuole2
Annotation ExtensionEvidenceWith/FromReference
cellular response to nitrogen starvation86
Annotation ExtensionEvidenceWith/FromReference
L-alpha-amino acid transmembrane transport9
Annotation ExtensionEvidenceWith/FromReference
NOT autophagy
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
fungal-type vacuole membrane91
Annotation ExtensionEvidenceWith/FromReference
integral component of fungal-type vacuolar membrane6
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000085sensitive to camptothecincompetitive growth assay evidenceatg22ΔNullPMID:20537132211
FYPO:0002060viable vegetative cell populationMicroscopyatg22ΔNullPMID:204732893759
Microscopyatg22ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopyatg22ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1836206838256

UTRs

Region Coordinates Reference
five_prime_UTR836206..836382PMID:21511999
three_prime_UTR837973..838256PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF11700 Pfam IPR024671 Autophagy-related protein 22-like 13 519 1
TMhelix TMHMM 85 107 959
TMhelix TMHMM 365 387 959
TMhelix TMHMM 494 516 959
TMhelix TMHMM 268 285 959
TMhelix TMHMM 240 262 959
TMhelix TMHMM 465 487 959
TMhelix TMHMM 139 161 959
TMhelix TMHMM 431 450 959
TMhelix TMHMM 21 43 959
TMhelix TMHMM 116 135 959
TMhelix TMHMM 397 419 959
1.20.1250.20 Gene3D 332 516 72
SSF103473 SuperFamily IPR016196 Major facilitator superfamily domain, general substrate transporter 239 521 76

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 110.85 Da
Charge 2.00
Isoelectric point 6.74
Molecular weight 58.64 kDa
Number of residues 529
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS529PMID:247631071670
experimental evidenceS3PMID:24763107
present during mitotic M phaseexperimental evidenceS529PMID:21712547
present during mitotic M phaseexperimental evidenceS527PMID:21712547
present during mitotic M phaseexperimental evidenceS3PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1760.07during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
10755.69during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.8during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only623
conserved in fungi4600
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
cdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
ddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
slx4structure-specific endonuclease subunit Slx4 Negative GeneticPMID:22681890
pob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
par1protein phosphatase regulatory subunit Par1 Positive GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
cid12poly(A) polymerase Cid12 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC2G11.13 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC2G11.13 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC2G11.13 BioGRID Interaction Datasets
Expression Viewer SPAC2G11.13 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC2G11.13 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC2G11.13 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC2G11.13 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC2G11.13 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC2G11.13 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC2G11.13 Cell Cycle Data
GEO SPAC2G11.13 GEO profiles
PInt SPAC2G11.13 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC2G11.13 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC2G11.13 Fission yeast phenotypic data & analysis
SPD / RIKEN25/25F02Orfeome Localization Data
UniProtKB/SwissProtQ09812Autophagy-related protein 22
ModBaseQ09812Database of comparative protein structure models
STRINGQ09812Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593093vacuolar amino acid efflux transporter Atg22
RefSeq mRNANM_001018491972h- vacuolar amino acid efflux transporter Atg22 (atg22), mRNA
European Nucleotide ArchiveCAA91178.1ENA Protein Mapping
UniParcUPI000013A00BUniProt Archive

Literature for atg22

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014