nup124 (SPAC30D11.04c)


Gene Standard Namenup124 Characterisation Statuspublished
Systematic IDSPAC30D11.04c Feature Typeprotein coding
Synonyms Name Description
Productnucleoporin Nup124 Product Size1159aa, 123.97 kDa
Genomic Location Chromosome I, 1112170-1115868 (3699nt); CDS:1112214-1115736 (3523nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005515protein bindingIPIcut15PMID:17615301861
IPIimp1PMID:17615301
IPIkap95PMID:17615301
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0051170nuclear importIMPPMID:1761530115
IMPPMID:15659641
GO:0000054ribosomal subunit export from nucleusISOSGD:S000005624GO_REF:000002429
GO:0032197transposition, RNA-mediatedIMPPMID:104097641
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005635nuclear envelopeIDAPMID:16823372137
GO:0005643nuclear pore54
during mitotic cell cycleIDAPMID:20970342
during meiotic cell cycleIDAPMID:20970342
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001701sensitive to bortezomib256
expressivity FYPO_EXT:0000002Cell growth assaynup124ΔNullPECO:0000005, PECO:0000137PMID:21760946
FYPO:0000085sensitive to camptothecincompetitive growth assay evidencenup124ΔNullPMID:20537132211
expressivity FYPO_EXT:0000003Cell growth assaynup124ΔNullPECO:0000137, PECO:0000005PMID:19264558
FYPO:0002060viable vegetative cell populationMicroscopynup124ΔNullPMID:204732893730
Microscopynup124ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopynot recorded (unrecorded)PMID:10409764

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001317normal RNA level during vegetative growth63
affecting mei4Transcript expression level evidencenup124ΔNullPMID:23658229
affecting rec8Transcript expression level evidencenup124ΔNullPMID:23658229
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopynup124ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
111121701112464
211125081115868

UTRs

Region Coordinates Reference
five_prime_UTR1112170..1112213PMID:21511999
three_prime_UTR1115737..1115868PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF10599 Pfam IPR018892 Retro-transposon transporting, conserved site 1073 1158 1
PF08604 Pfam IPR013913 Nucleoporin, Nup153-like 112 562 1
PTHR23193:SF22 HMMPANTHER 267 1147 1
PTHR23193 HMMPANTHER 267 1147 1

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000574FG repeatTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000574

Protein Properties

Ave. residue weight 106.97 Da
Charge 38.50
Isoelectric point 10.26
Molecular weight 123.97 kDa
Number of residues 1159
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS563PMID:247631071669
S380PMID:24763107
level fluctuates during mitotic cell cycleS549PMID:24763107
S896PMID:24763107
level fluctuates during mitotic cell cycleS244PMID:24763107
S215PMID:24763107
S209PMID:24763107
S207PMID:24763107
present during cellular response to thiabendazoleIDAS465PMID:18257517
present during cellular response to thiabendazoleIDAS211PMID:18257517
S219PMID:24763107
present during mitotic M phaseS467PMID:21712547
present during mitotic M phaseS146PMID:21712547
present during mitotic M phaseS549PMID:21712547
present during mitotic M phaseS235PMID:21712547
present during mitotic M phaseS465PMID:21712547
S465PMID:24763107
present during mitotic M phaseS380PMID:21712547
present during cellular response to thiabendazoleIDAS386PMID:18257517
present during cellular response to thiabendazoleIDAS209PMID:18257517
present during cellular response to thiabendazoleIDAS380PMID:18257517
present during mitotic M phaseS145PMID:21712547
S992PMID:24763107
present during mitotic M phaseS209PMID:21712547
present during mitotic M phaseS215PMID:21712547
present during mitotic M phaseS124PMID:21712547
present during mitotic M phaseS207PMID:21712547
present during mitotic M phaseS413PMID:21712547
present during mitotic M phaseS563PMID:21712547
present during mitotic M phaseS211PMID:21712547
present during mitotic M phaseS697PMID:21712547
present during mitotic M phaseS244PMID:21712547
present during mitotic M phaseS241PMID:21712547
MOD:00047O-phospho-L-threonineT429PMID:24763107689
T162PMID:24763107
present during mitotic M phaseT126PMID:21712547
T72PMID:24763107
present during mitotic M phaseT429PMID:21712547
present during mitotic M phaseT162PMID:21712547
present during mitotic M phaseT76PMID:21712547
present during mitotic M phaseT406PMID:21712547
present during mitotic M phaseT72PMID:21712547
MOD:00696phosphorylated residueNASPMID:182575171921
NASPMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
5760during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
5405during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
5738during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
6078during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
5947.63during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
5455during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
1390.42during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
6.9during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
2.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
swd2Set1C complex subunit Swd2.1 Negative GeneticPMID:22681890
pmc1mediator complex subunit Pmc1 Negative GeneticPMID:22681890
hip1hira protein, histone chaperone Hip1 Positive GeneticPMID:22681890
cut15karyopherin Cut15 Negative GeneticPMID:22681890
rik1silencing protein Rik1 Negative GeneticPMID:22681890
set1histone lysine methyltransferase Set1 Negative GeneticPMID:22681890
bdc1bromodomain containing protein 1, Bdc1 Negative GeneticPMID:22681890
ago1argonaute Positive GeneticPMID:22681890
vps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
swd3WD repeat protein Swd3 Negative GeneticPMID:22681890
clr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:22681890
sty1MAP kinase Sty1 Negative GeneticPMID:22681890
nup132nucleoporin Nup132 Negative GeneticPMID:22681890
rpa12DNA-directed RNA polymerase complex I subunit Rpa12 Negative GeneticPMID:22681890
mid1medial ring protein Mid1 Negative GeneticPMID:22681890
srp1SR family protein, human SRFS2 ortholog Srp1 Negative GeneticPMID:22681890
swd1Set1C complex subunit Swd1 Negative GeneticPMID:22681890
rxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
atg1autophagy and CVT pathway serine/threonine protein kinase Atg1 Positive GeneticPMID:22681890
not3CCR4-Not complex subunit Not3/5 (predicted) Negative GeneticPMID:22681890
clp1Cdc14-related protein phosphatase Clp1/Flp1 Negative GeneticPMID:22681890
sap14U2 snRNP-associated protein SF3B14 Sap14 Negative GeneticPMID:22681890
SPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:22681890
raf2Rik1-associated factor Raf2 Negative GeneticPMID:22681890
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:22681890
pck1protein kinase C (PKC)-like Pck1 Negative GeneticPMID:22681890
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
dsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
far10SIP/FAR complex FHA domain subunit Far10/Csc1 Negative GeneticPMID:22681890
SPBC29A3.09cAAA family ATPase Gcn20 (predicted) Negative GeneticPMID:22681890
nup61nucleoporin Nup61 Negative GeneticPMID:22681890
Phenotypic EnhancementPMID:19547744
SPAC1486.03cRNA-binding splicing factor (predicted) Positive GeneticPMID:22681890
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
mug154conserved fungal protein Negative GeneticPMID:22681890
par1protein phosphatase regulatory subunit Par1 Negative GeneticPMID:22681890
xks1xylulose kinase Xks1 (predicted) Positive GeneticPMID:22681890
dmc1RecA family ATPase Dmc1 Positive GeneticPMID:22681890
tom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
arp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
php5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
cwf15complexed with Cdc5 protein Cwf15 Negative GeneticPMID:22681890
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Positive GeneticPMID:22681890
SPBC1861.07elongin C (predicted) Negative GeneticPMID:22681890
nrm1MBF complex corepressor Nrm1 Negative GeneticPMID:22681890
hip4histone promoter control protein Hip4 Positive GeneticPMID:22681890
mcs2TFIIH complex cyclin Mcs2 Negative GeneticPMID:22681890
ngg1SAGA complex subunit Ngg1 Negative GeneticPMID:22681890
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Positive GeneticPMID:22681890
sif1Sad1 interacting factor 1 Negative GeneticPMID:22681890
spf1Set1C PHD Finger protein Spf1 Negative GeneticPMID:22681890
amk2AMP-activated protein kinase beta subunit Amk2 Negative GeneticPMID:22681890
sgf29SAGA complex subunit Sgf29 Negative GeneticPMID:22681890
ark1aurora-B kinase Ark1 Negative GeneticPMID:22681890
ssp2serine/threonine protein kinase Ssp2 Negative GeneticPMID:22681890
air1zinc knuckle TRAMP complex subunit Air1 Positive GeneticPMID:22681890
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
cut3condensin complex subunit Cut3 Negative GeneticPMID:22681890
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
prw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
cid12poly(A) polymerase Cid12 Positive GeneticPMID:22681890
vps5retromer complex subunit Vps5 Negative GeneticPMID:22681890
elp2elongator complex subunit Elp2 (predicted) Positive GeneticPMID:22681890
SPAC3A11.13prefoldin subunit 6 (predicted) Negative GeneticPMID:22681890
ams2cell cycle regulated GATA-type transcription factor Ams2 Positive GeneticPMID:22681890
pht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:22681890
swc3Swr1 complex subunit Swc3 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC30D11.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC30D11.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC30D11.04c BioGRID Interaction Datasets
Expression Viewer SPAC30D11.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC30D11.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC30D11.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC30D11.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC30D11.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC30D11.04c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC30D11.04c Cell Cycle Data
GEO SPAC30D11.04c GEO profiles
PInt SPAC30D11.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC30D11.04c Peptides identified in tandem mass spectrometry proteomics experiments
WikiGene2542254nucleoporin Nup124
EntrezGene2542254nucleoporin Nup124
SPD / RIKEN48/48D05Orfeome Localization Data
UniProtKB/SwissProtQ09904Nucleoporin nup124
ModBaseQ09904Database of comparative protein structure models
STRINGQ09904Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593213nucleoporin Nup124
RefSeq mRNANM_001018609972h- nucleoporin Nup124 (nup124), mRNA
European Nucleotide ArchiveAAF90179ENA Protein Mapping
European Nucleotide ArchiveAAF90179.1ENA Protein Mapping
European Nucleotide ArchiveCAA91890ENA Protein Mapping
European Nucleotide ArchiveCAA91890.1ENA Protein Mapping
UniParcUPI000012FC0FUniProt Archive

Literature for nup124

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014