nup124 (SPAC30D11.04c)


Gene Standard Namenup124 Characterisation Statuspublished
Systematic IDSPAC30D11.04c Feature Typeprotein coding
Synonyms Name DescriptionNUclear Pore factor
Productnucleoporin Nup124 Product Size1159aa, 123.97 kDa
Genomic Location Chromosome I, 1112170-1115868 (3699nt); CDS:1112214-1115736 (3523nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding831
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
nuclear import18
Annotation ExtensionEvidenceWith/FromReference
ribosomal subunit export from nucleus29
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nuclear envelope137
Annotation ExtensionEvidenceWith/FromReference
nuclear pore53
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001701sensitive to bortezomib256
expressivity FYPO_EXT:0000002Cell growth assaynup124ΔNullPECO:0000005, PECO:0000137PMID:21760946
FYPO:0000085sensitive to camptothecin211
expressivity FYPO_EXT:0000003Cell growth assaynup124ΔNullPECO:0000137, PECO:0000005PMID:19264558
competitive growth assay evidencenup124ΔNullPMID:20537132
FYPO:0002060viable vegetative cell populationMicroscopynup124ΔNullPMID:204732893759
Microscopynup124ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001317normal RNA level during vegetative growth80
affecting mei4Transcript expression level evidencenup124ΔNullPMID:23658229
affecting rec8Transcript expression level evidencenup124ΔNullPMID:23658229
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopynup124ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
111121701112464
211125081115868

UTRs

Region Coordinates Reference
five_prime_UTR1112170..1112213PMID:21511999
three_prime_UTR1115737..1115868PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF10599 Pfam IPR018892 Retro-transposon transporting, conserved site 1073 1158 1
PF08604 Pfam IPR013913 Nucleoporin, Nup153-like 112 562 1
PTHR23193 HMMPANTHER IPR026054 Nuclear pore complex protein 169 1157 2
PTHR23193:SF13 HMMPANTHER 169 1157 1

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000574FG repeatTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000574

Protein Properties

Ave. residue weight 106.97 Da
Charge 38.50
Isoelectric point 10.26
Molecular weight 123.97 kDa
Number of residues 1159
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS380PMID:247631071670
level fluctuates during mitotic cell cycleexperimental evidenceS549PMID:24763107
present during mitotic M phaseexperimental evidenceS211PMID:21712547
present during mitotic M phaseexperimental evidenceS215PMID:21712547
present during mitotic M phaseexperimental evidenceS413PMID:21712547
present during mitotic M phaseexperimental evidenceS697PMID:21712547
present during mitotic M phaseexperimental evidenceS145PMID:21712547
present during mitotic M phaseexperimental evidenceS563PMID:21712547
experimental evidenceS896PMID:24763107
experimental evidenceS992PMID:24763107
present during mitotic M phaseexperimental evidenceS549PMID:21712547
present during mitotic M phaseexperimental evidenceS380PMID:21712547
present during mitotic M phaseexperimental evidenceS146PMID:21712547
present during mitotic M phaseexperimental evidenceS465PMID:21712547
present during mitotic M phaseexperimental evidenceS235PMID:21712547
present during mitotic M phaseexperimental evidenceS241PMID:21712547
present during mitotic M phaseexperimental evidenceS244PMID:21712547
present during mitotic M phaseexperimental evidenceS124PMID:21712547
present during mitotic M phaseexperimental evidenceS207PMID:21712547
present during mitotic M phaseexperimental evidenceS209PMID:21712547
experimental evidenceS209PMID:24763107
experimental evidenceS215PMID:24763107
experimental evidenceS563PMID:24763107
experimental evidenceS207PMID:24763107
experimental evidenceS219PMID:24763107
level fluctuates during mitotic cell cycleexperimental evidenceS244PMID:24763107
experimental evidenceS465PMID:24763107
present during cellular response to thiabendazoleIDAS211PMID:18257517
present during cellular response to thiabendazoleIDAS380PMID:18257517
present during cellular response to thiabendazoleIDAS209PMID:18257517
present during cellular response to thiabendazoleIDAS386PMID:18257517
present during mitotic M phaseexperimental evidenceS467PMID:21712547
present during cellular response to thiabendazoleIDAS465PMID:18257517
MOD:00047O-phospho-L-threonineexperimental evidenceT76PMID:21712547692
present during mitotic M phase
present during mitotic M phaseexperimental evidenceT406PMID:21712547
present during mitotic M phaseexperimental evidenceT72PMID:21712547
experimental evidenceT72PMID:24763107
present during mitotic M phaseexperimental evidenceT126PMID:21712547
present during mitotic M phaseexperimental evidenceT162PMID:21712547
present during mitotic M phaseexperimental evidenceT429PMID:21712547
experimental evidenceT162PMID:24763107
experimental evidenceT429PMID:24763107
MOD:00696phosphorylated residueNASPMID:182575171922
NASPMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5760during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
5405during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
5738during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
6078during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
5947.63during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
5455during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1390.42during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
6.9during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
2.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
swd2Set1C complex subunit Swd2.1 Negative GeneticPMID:22681890
pmc1mediator complex subunit Pmc1 Negative GeneticPMID:22681890
hip1hira protein, histone chaperone Hip1 Positive GeneticPMID:22681890
cut15karyopherin Cut15 Negative GeneticPMID:22681890
rik1silencing protein Rik1 Negative GeneticPMID:22681890
set1histone lysine methyltransferase Set1 Negative GeneticPMID:22681890
bdc1bromodomain containing protein 1, Bdc1 Negative GeneticPMID:22681890
ago1argonaute Positive GeneticPMID:22681890
vps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
swd3WD repeat protein Swd3 Negative GeneticPMID:22681890
clr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:22681890
sty1MAP kinase Sty1 Negative GeneticPMID:22681890
nup132nucleoporin Nup132 Negative GeneticPMID:22681890
rpa12DNA-directed RNA polymerase complex I subunit Rpa12 Negative GeneticPMID:22681890
xap5xap-5-like protein Negative GeneticPMID:24957674
mid1medial ring protein Mid1 Negative GeneticPMID:22681890
srp1SR family protein, human SRFS2 ortholog Srp1 Negative GeneticPMID:22681890
swd1Set1C complex subunit Swd1 Negative GeneticPMID:22681890
rxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
atg1autophagy and CVT pathway serine/threonine protein kinase Atg1 Positive GeneticPMID:22681890
not3CCR4-Not complex subunit Not3/5 (predicted) Negative GeneticPMID:22681890
clp1Cdc14-related protein phosphatase Clp1/Flp1 Negative GeneticPMID:22681890
sap14U2 snRNP-associated protein SF3B14 Sap14 Negative GeneticPMID:22681890
SPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:22681890
raf2Rik1-associated factor Raf2 Negative GeneticPMID:22681890
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:22681890
pck1protein kinase C (PKC)-like Pck1 Negative GeneticPMID:22681890
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
dsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
far10SIP/FAR complex FHA domain subunit Far10/Csc1 Negative GeneticPMID:22681890
SPBC29A3.09cAAA family ATPase Gcn20 (predicted) Negative GeneticPMID:22681890
nup61nucleoporin Nup61 Negative GeneticPMID:22681890
Phenotypic EnhancementPMID:19547744
SPAC1486.03cRNA-binding splicing factor (predicted) Positive GeneticPMID:22681890
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
mug154conserved fungal protein Negative GeneticPMID:22681890
par1protein phosphatase regulatory subunit Par1 Negative GeneticPMID:22681890
xks1xylulose kinase Xks1 (predicted) Positive GeneticPMID:22681890
dmc1RecA family ATPase Dmc1 Positive GeneticPMID:22681890
tom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
arp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
php5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
cwf15complexed with Cdc5 protein Cwf15 Negative GeneticPMID:22681890
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Positive GeneticPMID:22681890
SPBC1861.07elongin C (predicted) Negative GeneticPMID:22681890
nrm1MBF complex corepressor Nrm1 Negative GeneticPMID:22681890
hip4histone promoter control protein Hip4 Positive GeneticPMID:22681890
mcs2TFIIH complex cyclin Mcs2 Negative GeneticPMID:22681890
ngg1SAGA complex subunit Ngg1 Negative GeneticPMID:22681890
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Positive GeneticPMID:22681890
sif1Sad1 interacting factor 1 Negative GeneticPMID:22681890
spf1Set1C PHD Finger protein Spf1 Negative GeneticPMID:22681890
amk2AMP-activated protein kinase beta subunit Amk2 Negative GeneticPMID:22681890
sgf29SAGA complex subunit Sgf29 Negative GeneticPMID:22681890
ark1aurora-B kinase Ark1 Negative GeneticPMID:22681890
ssp2serine/threonine protein kinase Ssp2 Negative GeneticPMID:22681890
air1zinc knuckle TRAMP complex subunit Air1 Positive GeneticPMID:22681890
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
cut3condensin complex subunit Cut3 Negative GeneticPMID:22681890
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
prw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
cid12poly(A) polymerase Cid12 Positive GeneticPMID:22681890
vps5retromer complex subunit Vps5 Negative GeneticPMID:22681890
elp2elongator complex subunit Elp2 (predicted) Positive GeneticPMID:22681890
SPAC3A11.13prefoldin subunit 6 (predicted) Negative GeneticPMID:22681890
ams2cell cycle regulated GATA-type transcription factor Ams2 Positive GeneticPMID:22681890
pht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:22681890
swc3Swr1 complex subunit Swc3 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC30D11.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC30D11.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC30D11.04c BioGRID Interaction Datasets
Expression Viewer SPAC30D11.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC30D11.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC30D11.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC30D11.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC30D11.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC30D11.04c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC30D11.04c Cell Cycle Data
GEO SPAC30D11.04c GEO profiles
PInt SPAC30D11.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC30D11.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC30D11.04c Fission yeast phenotypic data & analysis
SPD / RIKEN48/48D05Orfeome Localization Data
UniProtKB/SwissProtQ09904Nucleoporin nup124
ModBaseQ09904Database of comparative protein structure models
STRINGQ09904Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593213nucleoporin Nup124
RefSeq mRNANM_001018609972h- nucleoporin Nup124 (nup124), mRNA
European Nucleotide ArchiveAAF90179.1ENA Protein Mapping
European Nucleotide ArchiveCAA91890.1ENA Protein Mapping
UniParcUPI000012FC0FUniProt Archive

Literature for nup124

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014