cuf1 (SPAC31A2.11c)

Gene Standard Namecuf1 Characterisation Statuspublished
Systematic IDSPAC31A2.11c Feature Typeprotein coding
Synonyms Name Description
Productnutritional copper sensing transcription factor Cuf1 Product Size411aa, 45.47 kDa
Genomic Location Chromosome I, 409872-407714 (2159nt); CDS:409265-408030 (1236nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
GO Cellular Component
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
abolished cell population growth on glycerol carbon sourcecuf1ΔNull65
decreased cell population growth on glucose carbon source154
expressivity FYPO_EXT:0000003cuf1ΔNull
decreased mating efficiencycuf1ΔNull247
decreased vegetative cell population growthcuf1ΔNull400
normal growth on glycerol carbon sourcecuf1ΔNull8
sensitive to 4-nitroquinoline N-oxide159
expressivity FYPO_EXT:0000003cuf1ΔNull
sensitive to cadmium246
expressivity FYPO_EXT:0000002cuf1ΔNull
sensitive to hydrogen peroxidecuf1ΔNull137
sensitive to iron ion starvationcuf1ΔNull4
viable vegetative cell populationcuf1ΔNull3781

Cell Phenotype

Term NameAlleleExpressionCount
abnormal microtubule cytoskeleton morphology during mitotic interphase186
penetrance FYPO_EXT:0000002cuf1ΔNull
abolished primary amine oxidase activitycuf1ΔNull4
decreased RNA level during cellular response to copper ion1
affecting fip1cuf1ΔNull
affecting fio1cuf1ΔNull
decreased RNA level during cellular response to copper ion starvation2
affecting ctr6cuf1ΔNull
decreased superoxide dismutase activitycuf1ΔNull4
increased RNA level during vegetative growth171
affecting fip1cuf1ΔNull
affecting fio1cuf1ΔNull
long cytoplasmic microtubules19
penetrance FYPO_EXT:0000003, expressivity FYPO_EXT:0000003cuf1ΔNull
normal RNA level during vegetative growth91
affecting cao1cuf1ΔNull
affecting cao2cuf1ΔNull
RNA absent from cell during cellular response to copper ion starvation1
affecting ctr4cuf1ΔNull
RNA absent from cell during vegetative growth17
affecting ctr4cuf1ΔNull
viable elongated vegetative cell231
penetrance FYPO_EXT:0000003, expressivity FYPO_EXT:0000002cuf1ΔNull
viable vegetative cell with abnormal cell shape472
penetrance FYPO_EXT:0000001cuf1ΔNull
viable vegetative cell with normal cell morphology3092
penetrance FYPO_EXT:0000001cuf1ΔNull
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00649 Pfam IPR001083 Copper fist DNA-binding 1 39 2
SM00412 SMART IPR001083 Copper fist DNA-binding 1 40 2
SM01090 SMART 1 40 2
PS01119 Prosite Patterns IPR001083 Copper fist DNA-binding 1 39 2
PS50073 Prosite Profiles IPR001083 Copper fist DNA-binding 1 40 2
1co4A00 Gene3D IPR001083 Copper fist DNA-binding 1 42 2
0036077 SuperFamily IPR001083 Copper fist DNA-binding 1 41 2
PR00617 PRINTS IPR001083 Copper fist DNA-binding 14 26 2
PR00617 PRINTS IPR001083 Copper fist DNA-binding 1 13 2
PR00617 PRINTS IPR001083 Copper fist DNA-binding 27 39 2

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount

Protein Properties

Ave. residue weight 110.64 Da
Charge 8.00
Isoelectric point 7.34
Molecular weight 45.47 kDa
Number of residues 411
Gene Expression

Qualitative Gene Expression

RNA levelpresent during GO:0072690reporter gene assay evidencePMID:17384198
decreased during GO:0071280reporter gene assay evidencePMID:17384198

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3839during GO:0072690PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
989.7during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.1during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
0.62during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633
functionally complements S. cerevisiae MAC1
deletion mutant expression profiling PMID:1747786378
target of leptomycin B PMID:173841982
Miscellaneous Functional Group
nucleocytoplasmic shuttling protein PMID:1646746914
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi only619
conserved in fungi4604
conserved in eukaryotes4516

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
cuf1nutritional copper sensing transcription factor Cuf1 Two-hybridPMID:23695164
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
sre2membrane-tethered transcription factor Sre2 Positive GeneticPMID:21504829
mei4meiotic forkhead transcription factor Mei4 Phenotypic EnhancementPMID:24569997
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Positive GeneticPMID:21504829
dsc2Golgi Dsc E3 ligase complex subunit Dsc2 Positive GeneticPMID:21504829
dis2serine/threonine protein phosphatase PP1 subfamily, Dis2 Synthetic Growth DefectPMID:21965289
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Positive GeneticPMID:21504829
External References
Database Identifier Description
NBRP SPAC31A2.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC31A2.11c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC31A2.11c BioGRID Interaction Datasets
Expression Viewer SPAC31A2.11c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC31A2.11c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC31A2.11c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC31A2.11c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC31A2.11c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC31A2.11c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC31A2.11c Cell Cycle Data
GEO SPAC31A2.11c GEO profiles
PInt SPAC31A2.11c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC31A2.11c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC31A2.11c Fission yeast phenotypic data & analysis
SPD / RIKEN19/19G01Orfeome Localization Data
UniProtKB/SwissProtQ09728Metal-binding regulatory protein cuf1
ModBaseQ09728Database of comparative protein structure models
STRINGQ09728Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_592923nutritional copper sensing transcription factor Cuf1
RefSeq mRNANM_001018324972h- nutritional copper sensing transcription factor Cuf1 (cuf1), mRNA
European Nucleotide ArchiveAAD51063.1ENA Protein Mapping
European Nucleotide ArchiveCAA90469.1ENA Protein Mapping
European Nucleotide ArchiveCAB52304.1ENA Protein Mapping
UniParcUPI00001286E0UniProt Archive

Literature for cuf1

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015