cuf1 (SPAC31A2.11c)


Gene Standard Namecuf1 Characterisation Statuspublished
Systematic IDSPAC31A2.11c Feature Typeprotein coding
Synonyms Name Description
Productnutritional copper sensing transcription factor Cuf1 Product Size411aa, 45.47 kDa
Genomic Location Chromosome I, 409872-407714 (2159nt); CDS:409265-408030 (1236nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
GO Cellular Component
Term NameCount
cytoplasm4205
Annotation ExtensionEvidenceWith/FromReference
nucleus2740
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001934abolished cell population growth on glycerol carbon sourceCell growth assaycuf1ΔNullPECO:0000072PMID:1866526861
FYPO:0001355decreased vegetative cell population growthCell growth assaycuf1ΔNullPECO:0000126PMID:18665268381
FYPO:0001098sensitive to 4-nitroquinoline N-oxide159
expressivity FYPO_EXT:0000003Cell growth assaycuf1ΔNullPECO:0000137, PECO:0000005PMID:19264558
FYPO:0000096sensitive to cadmium246
expressivity FYPO_EXT:0000002Cell growth assaycuf1ΔNullPECO:0000102, PECO:0000005, PECO:0000137PMID:18684775
FYPO:0000087sensitive to hydrogen peroxideCell growth assaycuf1ΔNullPECO:0000137PMID:18665268131
FYPO:0002060viable vegetative cell populationMicroscopycuf1ΔNullPECO:0000005, PECO:0000137PMID:236978063759
Microscopycuf1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0003625abnormal microtubule cytoskeleton morphology during mitotic interphase186
penetrance FYPO_EXT:0000002experimental phenotypic evidencecuf1ΔNullPECO:0000005, PECO:0000103, PECO:0000137DOI:10.1016/j.devcel.2014.09.005
FYPO:0000708decreased mating efficiencyhigh throughput nucleotide sequencing assay evidencecuf1ΔNullPECO:0000015PMID:23950735240
FYPO:0003280decreased RNA level during cellular response to copper ion starvation2
affecting ctr6Transcript expression level evidencecuf1ΔNullPMID:12244050
FYPO:0000233long cytoplasmic microtubules19
expressivity FYPO_EXT:0000003
penetrance FYPO_EXT:0000003
experimental phenotypic evidencecuf1ΔNullPECO:0000137, PECO:0000005, PECO:0000103DOI:10.1016/j.devcel.2014.09.005
FYPO:0001492viable elongated vegetative cell221
expressivity FYPO_EXT:0000002
penetrance FYPO_EXT:0000003
experimental phenotypic evidencecuf1ΔNullPECO:0000137, PECO:0000005, PECO:0000103DOI:10.1016/j.devcel.2014.09.005
FYPO:0002197viable vegetative cell with abnormal cell shape467
penetrance FYPO_EXT:0000001experimental phenotypic evidencecuf1ΔNullPECO:0000005, PECO:0000103, PECO:0000137DOI:10.1016/j.devcel.2014.09.005
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopycuf1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1409872407714

UTRs

Region Coordinates Reference
five_prime_UTR409872..409266PMID:21511999
three_prime_UTR408029..407714PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00649 Pfam IPR001083 Copper fist DNA-binding 1 39 2
SM00412 SMART IPR001083 Copper fist DNA-binding 1 40 2
SM01090 SMART 1 40 2
PS01119 Prosite Patterns IPR001083 Copper fist DNA-binding 1 39 2
PS50073 Prosite Profiles IPR001083 Copper fist DNA-binding 1 40 2
1co4A00 Gene3D IPR001083 Copper fist DNA-binding 1 42 2
0036077 SuperFamily IPR001083 Copper fist DNA-binding 1 41 2
PR00617 PRINTS IPR001083 Copper fist DNA-binding 1 13 2
PR00617 PRINTS IPR001083 Copper fist DNA-binding 27 39 2
PR00617 PRINTS IPR001083 Copper fist DNA-binding 14 26 2

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001096polypeptide_copper_ion_contact_sitePMID:164674691
SO:0001528nuclear_localization_signalPMID:1646746920
SO:0001531nuclear_export_signalPMID:173841987

Protein Properties

Ave. residue weight 110.64 Da
Charge 8.00
Isoelectric point 7.34
Molecular weight 45.47 kDa
Number of residues 411
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA leveldecreased during GO:0071280reporter gene assay evidencePMID:17384198
present during GO:0072690reporter gene assay evidencePMID:17384198

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
989.7during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
3839during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.1during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.62during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complements S. cerevisiae MAC1
Misc
DescriptionQualifierReferenceCount
deletion mutant expression profiling PMID:1747786378
target of leptomycin B PMID:173841982
Miscellaneous Functional Group
DescriptionQualifierReferenceCount
nucleocytoplasmic shuttling protein PMID:1646746914
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only623
conserved in fungi4600
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
cuf1nutritional copper sensing transcription factor Cuf1 Two-hybridPMID:23695164
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
sre2membrane-tethered transcription factor Sre2 Positive GeneticPMID:21504829
mei4meiotic forkhead transcription factor Mei4 Phenotypic EnhancementPMID:24569997
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Positive GeneticPMID:21504829
dsc2Golgi Dsc E3 ligase complex subunit Dsc2 Positive GeneticPMID:21504829
dis2serine/threonine protein phosphatase PP1 subfamily, Dis2 Synthetic Growth DefectPMID:21965289
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Positive GeneticPMID:21504829
External References
Database Identifier Description
NBRP SPAC31A2.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC31A2.11c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC31A2.11c BioGRID Interaction Datasets
Expression Viewer SPAC31A2.11c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC31A2.11c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC31A2.11c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC31A2.11c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC31A2.11c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC31A2.11c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC31A2.11c Cell Cycle Data
GEO SPAC31A2.11c GEO profiles
PInt SPAC31A2.11c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC31A2.11c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC31A2.11c Fission yeast phenotypic data & analysis
SPD / RIKEN19/19G01Orfeome Localization Data
UniProtKB/SwissProtQ09728Metal-binding regulatory protein cuf1
ModBaseQ09728Database of comparative protein structure models
STRINGQ09728Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_592923nutritional copper sensing transcription factor Cuf1
RefSeq mRNANM_001018324972h- nutritional copper sensing transcription factor Cuf1 (cuf1), mRNA
European Nucleotide ArchiveAAD51063.1ENA Protein Mapping
European Nucleotide ArchiveCAA90469.1ENA Protein Mapping
European Nucleotide ArchiveCAB52304.1ENA Protein Mapping
UniParcUPI00001286E0UniProt Archive

Literature for cuf1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014