cuf1 (SPAC31A2.11c)


Gene Standard Namecuf1 Characterisation Statuspublished
Systematic IDSPAC31A2.11c Feature Typeprotein coding
Synonyms Name Description
Productnutritional copper sensing transcription factor Cuf1 Product Size411aa, 45.47 kDa
Genomic Location Chromosome I, 409872-407714 (2159nt); CDS:409265-408030 (1236nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000978RNA polymerase II core promoter proximal region sequence-specific DNA bindingIDAPMID:1059391396
at CuREIDAPMID:12578838
GO:0005507copper ion bindingIMPPMID:1646746915
GO:0001202copper ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activityIDAPMID:125788381
regulates ctr4IMPPMID:12578838
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006879cellular iron ion homeostasisIMPPMID:1059391324
GO:0071280cellular response to copper ionIMPPMID:125788381
GO:0000122negative regulation of transcription from RNA polymerase II promoterIDAPMID:1059391374
GO:0045944positive regulation of transcription from RNA polymerase II promoterIDAPMID:1059391385
during cellular response to copper ion starvation, regulates ctr6IMPPMID:12244050
during cellular response to copper ion starvation, regulates ctr5IMPPMID:11274192
during cellular response to copper ion starvation, regulates ctr4IMPPMID:11274192
GO:0006366transcription from RNA polymerase II promoterIMPPMID:12578838130
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmIDAPMID:164674694198
GO:0005634nucleusIDAPMID:168233722730
during cellular response to copper ion starvationIDAPMID:16467469
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001934abolished cell population growth on glycerol carbon sourceCell growth assaycuf1ΔNullPECO:0000072PMID:1866526857
FYPO:0001355decreased vegetative cell population growthCell growth assaycuf1ΔNullPECO:0000126PMID:18665268201
FYPO:0001098sensitive to 4-nitroquinoline N-oxideCell growth assaycuf1ΔNullPECO:0000137, PECO:0000005PMID:19264558159
expressivity FYPO_EXT:0000003
FYPO:0000096sensitive to cadmiumcell growth assaycuf1ΔNullPECO:0000102, PECO:0000005, PECO:0000137PMID:18684775244
expressivity FYPO_EXT:0000002
FYPO:0000087sensitive to hydrogen peroxideCell growth assaycuf1ΔNullPECO:0000137PMID:18665268112
FYPO:0002060viable vegetative cell populationMicroscopycuf1ΔNullPMID:204732893755
Microscopycuf1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000708decreased mating efficiencyhigh throughput nucleotide sequencing assay evidencecuf1ΔNullPECO:0000015PMID:23950735222
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopycuf1ΔNullPECO:0000005, PECO:0000137PMID:236978063078
penetrance FYPO_EXT:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1409872407714

UTRs

Region Start End Reference
three_prime_UTR408029407714PMID:21511999
five_prime_UTR409872409266PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00649 Pfam IPR001083 Copper fist DNA-binding 1 39 2
SM00412 SMART IPR001083 Copper fist DNA-binding 1 40 2
SM01090 SMART 1 40 2
PS50073 Prosite Profiles IPR001083 Copper fist DNA-binding 1 40 2
PS01119 Prosite Patterns IPR001083 Copper fist DNA-binding 1 39 2
G3DSA:3.90.430.10 Gene3D IPR001083 1 42 2
SSF57879 SuperFamily IPR001083 1 41 2
Low complexity (SEG) seg 321 334
Low complexity (SEG) seg 63 95
PR00617 PRINTS IPR001083 Copper fist DNA-binding 14 26 2
PR00617 PRINTS IPR001083 Copper fist DNA-binding 1 13 2
PR00617 PRINTS IPR001083 Copper fist DNA-binding 27 39 2

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001096polypeptide_copper_ion_contact_sitePMID:164674691
SO:0001528nuclear_localization_signalPMID:1646746921
SO:0001531nuclear_export_signalPMID:173841987

Protein Properties

Ave. residue weight 110.64 Da
Charge 8.00
Isoelectric point 7.34
Molecular weight 45.47 kDa
Number of residues 411
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelpresentreporter gene assay evidencePMID:17384198
during GO:0072690
decreasedreporter gene assay evidencePMID:17384198
during GO:0071280

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
989.7during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2.1during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.62during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReferenceCount
functionally complements S. cerevisiae MAC11
Misc
DescriptionQualifierReferenceCount
deletion mutant expression profiling PMID:1747786379
target of leptomycin B PMID:173841982
Miscellaneous Functional Group
DescriptionQualifierReferenceCount
nucleocytoplasmic shuttling protein PMID:1646746914
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi only603
conserved in fungi4564
conserved in eukaryotes4482
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
sre2membrane-tethered transcription factor Sre2 Positive GeneticPMID:21504829
dsc2Golgi Dsc E3 ligase complex subunit Dsc2 Positive GeneticPMID:21504829
dis2serine/threonine protein phosphatase PP1 Synthetic Growth DefectPMID:21965289
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Positive GeneticPMID:21504829
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Positive GeneticPMID:21504829
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
External References
Database Identifier Description
NBRP SPAC31A2.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC31A2.11c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC31A2.11c BioGRID Interaction Datasets
Expression Viewer SPAC31A2.11c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC31A2.11c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC31A2.11c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC31A2.11c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC31A2.11c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC31A2.11c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC31A2.11c Cell Cycle Data
GEO SPAC31A2.11c GEO profiles
PInt SPAC31A2.11c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2543198Cu metalloregulatory transcription factor Cuf1
EntrezGene2543198Cu metalloregulatory transcription factor Cuf1
SPD / RIKEN19/19G01Orfeome Localization Data
UniProtKB/SwissProtQ09728Metal-binding regulatory protein cuf1
ModBaseQ09728Database of comparative protein structure models
StringQ09728Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_592923Cu metalloregulatory transcription factor Cuf1
RefSeq mRNANM_001018324972h- Cu metalloregulatory transcription factor Cuf1 (cuf1), mRNA
European Nucleotide ArchiveAAD51063ENA Protein Mapping
European Nucleotide ArchiveCAA90469ENA Protein Mapping
European Nucleotide ArchiveCAB52304ENA Protein Mapping
UniParcUPI00001286E0UniProt Archive

Literature for cuf1

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014