cuf1 (SPAC31A2.11c)


Gene Standard Namecuf1 Characterisation Statuspublished
Systematic IDSPAC31A2.11c Feature Typeprotein coding
Synonyms Name Description
Productnutritional copper sensing transcription factor Cuf1 Product Size411aa, 45.47 kDa
Genomic Location Chromosome I, 409872-407714 (2159nt); CDS:409265-408030 (1236nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
GO Cellular Component
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
abolished cell population growth on glycerol carbon sourcecuf1Δ67
decreased cell population growth on glucose carbon sourcecuf1+ (wild type)319
decreased mating efficiencycuf1Δ272
decreased vegetative cell population growthcuf1Δ657
sensitive to 4-nitroquinoline N-oxidecuf1Δ159
sensitive to 5-fluorouracilcuf1Δ273
sensitive to cadmiumcuf1Δ249
sensitive to copperNESmut2 (L349A, L352A, I355A, L358A)11
M6 (C328A, C330A, C334A, C336A, C339A, H342A)
NESmut1 (L349A, L352A)
sensitive to hydrogen peroxidecuf1Δ154
viable vegetative cell populationcuf1Δ3840

Cell Phenotype

Term NameGenotypesCount
abnormal microtubule cytoskeleton morphology during mitotic interphasecuf1Δ186
abnormal septationcuf1+ (wild type)292
abolished primary amine oxidase activitycuf1Δ4
abolished protein binding126
affecting cuf1 and crm1NESmut2 (L349A, L352A, I355A, L358A)
decreased RNA level during cellular response to copper ion starvation2
affecting ctr6cuf1Δ
increased RNA level during cellular response to copper ion1
affecting ctr4NESmut1 (L349A, L352A)
affecting ctr4NESmut2 (L349A, L352A, I355A, L358A)
affecting ctr4M6 (C328A, C330A, C334A, C336A, C339A, H342A)
long cytoplasmic microtubulescuf1Δ23
normal RNA level during vegetative growth122
affecting cao1cuf1Δ
affecting cao2cuf1Δ
protein mislocalized to nucleus during cellular response to copper ion2
affects localization of cuf1NESmut1 (L349A, L352A)
affects localization of cuf1NESmut2 (L349A, L352A, I355A, L358A)
affects localization of cuf1M6 (C328A, C330A, C334A, C336A, C339A, H342A)
RNA absent from cell during cellular response to copper ion starvation1
affecting ctr5cuf1Δ
affecting ctr4cuf1Δ
viable elongated vegetative cellcuf1Δ318
cuf1+ (wild type)
viable vegetative cell with abnormal cell shapecuf1Δ700
viable vegetative cell with normal cell morphologycuf1Δ3100
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in cuf1 nutritional copper sensing transcription factor Cuf1
FYPO localization affected by mutation in crm1 importin family nuclear export receptor Crm1
FYPO localization affected by mutation in cuf1 nutritional copper sensing transcription factor Cuf1
GO transported by crm1 importin family nuclear export receptor Crm1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons409872..407714
mRNA409872..407714
5' UTR409872..409266PMID:21511999
CDS409265..408030
3' UTR408029..407714PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00649 Pfam IPR001083 Copper fist DNA-binding domain 2 39 2
SM00412 SMART IPR001083 Copper fist DNA-binding domain 1 40 2
SM01090 SMART Copper fist DNA-binding domain 1 40 2
PS50073 Prosite Profiles IPR001083 Copper fist DNA-binding domain 1 40 2
PS01119 Prosite Patterns IPR001083 Copper fist DNA-binding domain 1 39 2
PTHR28088 HMMPANTHER 2 388 2
PTHR28088:SF2 HMMPANTHER 2 388 2
1co4A00 Gene3D IPR001083 Copper fist DNA-binding domain 1 42 2
0036077 SuperFamily IPR001083 Copper fist DNA-binding domain 1 41 2
PR00617 PRINTS IPR001083 Copper fist DNA-binding domain 27 39 2
PR00617 PRINTS IPR001083 Copper fist DNA-binding domain 1 13 2
PR00617 PRINTS IPR001083 Copper fist DNA-binding domain 14 26 2

View domain organization at Pfam

Term IDTerm NameReferenceCount
SO:0001096polypeptide_copper_ion_contact_site1
residue C328-H342PMID:16467469
SO:0001528nuclear_localization_signal21
residue C11-C53PMID:16467469
SO:0001531nuclear_export_signal7
residue Y349-L358PMID:17384198

Protein Properties

Ave. residue weight 110.64 Da
Charge 8.00
Codon Adaptation Index 0.44
Isoelectric point 7.34
Molecular weight 45.47 kDa
Number of residues 411
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA leveldecreased during GO:0071280reporter gene assay evidencePMID:17384198
present during GO:0072690reporter gene assay evidencePMID:17384198

Quantitative Gene Expression

View graphical display of gene expression data for cuf1 (SPAC31A2.11c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3839during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
989.7during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.1during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.62during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complements S. cerevisiae MAC1
Misc
DescriptionQualifierReferenceCount
deletion mutant expression profiling PMID:1747786377
target of leptomycin B PMID:173841982
Miscellaneous Functional Group
DescriptionQualifierReferenceCount
nucleocytoplasmic shuttling protein PMID:1646746914
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only618
conserved in fungi4606
conserved in eukaryotes4516
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC31A2.11c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withcrm1importin family nuclear export receptor Crm1 Two-hybridPMID:17384198
binds DNA-binding domain construct withsfc7TFIIIC subunit Sfc7 (predicted) Two-hybridPMID:26771498
binds activation domain construct withcuf1nutritional copper sensing transcription factor Cuf1 Two-hybridPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC31A2.11c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Positive GeneticPMID:21504829
positive genetic interaction withsre2membrane-tethered transcription factor Sre2 Positive GeneticPMID:21504829
positive genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Positive GeneticPMID:21504829
positive genetic interaction withdsc2Golgi Dsc E3 ligase complex subunit Dsc2 Positive GeneticPMID:21504829
phenotype enhanced bymei4meiotic forkhead transcription factor Mei4 Phenotypic EnhancementPMID:24569997
synthetic growth defect withdis2serine/threonine protein phosphatase PP1, Dis2 Synthetic Growth DefectPMID:21965289
External References
Database Identifier Description
NBRP SPAC31A2.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC31A2.11c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC31A2.11c BioGRID Interaction Datasets
Expression Viewer SPAC31A2.11c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC31A2.11c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC31A2.11c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC31A2.11c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC31A2.11c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC31A2.11c Transcriptome Viewer (Bähler Lab)
GEO SPAC31A2.11c GEO profiles
PInt SPAC31A2.11c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC31A2.11c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC31A2.11c Fission yeast phenotypic data & analysis
Cyclebase SPAC31A2.11c.1 Cell Cycle Data
SPD / RIKEN19/19G01Orfeome Localization Data
UniProtKB/SwissProtQ09728Metal-binding regulatory protein cuf1
ModBaseQ09728Database of comparative protein structure models
STRINGQ09728Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_592923nutritional copper sensing transcription factor Cuf1
RefSeq mRNANM_001018324972h- nutritional copper sensing transcription factor Cuf1 (cuf1), mRNA

Literature for cuf1

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016