abo1 (SPAC31G5.19)


Gene Standard Nameabo1 Characterisation Statuspublished
Systematic IDSPAC31G5.19 Feature Typeprotein coding
Synonyms Name Description
ProductATPase with bromodomain protein Product Size1190aa, 135.35 kDa
Genomic Location Chromosome I, 3023051-3028158 (5108nt); CDS:3024418-3027990 (3573nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding559
Annotation ExtensionEvidenceWith/FromReference
ATPase activity240
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
chromatin remodeling156
Annotation ExtensionEvidenceWith/FromReference
nucleosome positioning7
Annotation ExtensionEvidenceWith/FromReference
regulation of transcription from RNA polymerase II promoter297
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased spore germination frequencyabo1Δ61
normal growth on amphotericin Babo1Δ17
sensitive to cobaltabo1Δ57
sensitive to doxorubicinabo1Δ95
sensitive to methyl methanesulfonateabo1Δ255
sensitive to micafunginabo1Δ119
sensitive to thiabendazoleabo1Δ264
slow vegetative cell population growthabo1Δ357
viable vegetative cell populationabo1Δ3837

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic cell cycleabo1Δ858
abnormal nucleosome positioningabo1Δ8
abnormal sporulation resulting in formation of ascus with fewer than four sporesabo1Δ76
decreased chromatin silencing at centromere inner repeatabo1Δ10
decreased chromatin silencing at centromere outer repeatabo1Δ45
decreased chromatin silencing at silent mating-type cassetteabo1Δ25
decreased level of histone H2A in cellabo1Δ1
decreased level of histone H3 at dh repeatabo1Δ2
decreased level of histone H3 in cellabo1Δ3
decreased transcript length4
affecting SPBC19C7.11abo1Δ
increased antisense RNA level9
affecting hrp1abo1Δ
affecting prp1abo1Δ
increased centromeric outer repeat transcript levelabo1Δ24
increased level of stress responsive gene mRNA during vegetative growthabo1Δ12
increased LTR-derived RNA levelabo1Δ4
increased minichromosome loss during vegetative growthabo1Δ66
increased subtelomere-derived RNA level5
affecting tlh1abo1Δ
normal conjugationabo1Δ49
normal histone H3-K9 dimethylation at centromereabo1Δ5
viable elongated vegetative cellabo1Δ317
viable vegetative cell with abnormal cell shapeabo1Δ699

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
abolished cell population growth at high temperatureabo1Δ, hht2Δ, hhf2Δ
decreased cell population growth on glucose carbon sourcenhp6Δ, abo1Δ
inviable cell populationabo1Δ, abo2Δ
abo1Δ, spt16-18
sensitive to doxorubicintop2-191, abo1Δ
sensitive to methyl methanesulfonateabo1Δ, hht2Δ, hhf2Δ
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3023051..3023169, 3023232..3028158
Intron3023170..3023231
5' UTR3023051..3023169, 3023232..3024417PMID:21511999
3' UTR3027991..3028158PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00004 Pfam IPR003959 ATPase, AAA-type, core 606 722 32
PF00004 Pfam IPR003959 ATPase, AAA-type, core 303 438 32
SM00382 SMART IPR003593 AAA+ ATPase domain 299 440 70
SM00382 SMART IPR003593 AAA+ ATPase domain 601 705 70
PS00674 Prosite Patterns IPR003960 ATPase, AAA-type, conserved site 408 426 19
PTHR23069:SF0 HMMPANTHER 128 827 2
PTHR23069:SF0 HMMPANTHER 849 1190 2
PTHR23069:SF0 HMMPANTHER 1 74 2
PTHR23069 HMMPANTHER 128 827 2
PTHR23069 HMMPANTHER 849 1190 2
PTHR23069 HMMPANTHER 1 74 2
1.10.8.60 Gene3D DNA helicase, Holliday junction RuvB type 447 529 35
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 264 446 288
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 590 745 288
1.20.920.10 Gene3D IPR001487 Bromodomain 864 911 10
SSF47370 SuperFamily IPR001487 Bromodomain 865 913 10
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 264 529 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 591 751 294

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000496bromodomainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000496
PBO:0000539AAA family ATPaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000539

Protein Properties

Ave. residue weight 113.74 Da
Charge -28.50
Codon Adaptation Index 0.41
Isoelectric point 4.99
Molecular weight 135.35 kDa
Number of residues 1190
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phaseS214
present during cellular response to thiabendazole, mitotic M phaseS1047
present during mitotic M phaseS972
present during cellular response to thiabendazoleS139
present during mitotic M phaseS212
S1047
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S214 PMID:21712547
present during cellular response to thiabendazole IDA S1047 PMID:18257517
present during mitotic M phase experimental evidence S1047 PMID:21712547
present during mitotic M phase experimental evidence S972 PMID:21712547
present during cellular response to thiabendazole IDA S139 PMID:18257517
present during mitotic M phase experimental evidence S212 PMID:21712547
experimental evidence S1047 PMID:24763107
phosphorylated residue 1930
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for abo1 (SPAC31G5.19)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1598.58during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
801.32during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.94during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancer148
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4605
conserved in eukaryotes4515
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC31G5.19 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byprf1RNA polymerase II associated Paf1 complex (predicted) Affinity Capture-MSPMID:24385927
affinity captured bytpr1RNA polymerase II associated Paf1 complex subunit Tpr1 Affinity Capture-MSPMID:24385927
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
binds activation domain construct withfta1kinetochore protein, CENP-L ortholog Fta1 Two-hybridPMID:26771498
binds activation domain construct withabo1ATPase with bromodomain protein Two-hybridPMID:26771498
binds activation domain construct withpci2TREX complex subunit Pci2 (predicted) Two-hybridPMID:26771498
binds DNA-binding domain construct withSPAC1142.04Noc complex subunit Noc2 family (predicted) Two-hybridPMID:26771498
affinity capturesspt16FACT complex subunit Spt16 Affinity Capture-WesternPMID:26582768
affinity capturespob3FACT complex subunit Pob3 Affinity Capture-WesternPMID:26582768
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC31G5.19 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
phenotype enhanced byhhf2histone H4 h4.2 Phenotypic EnhancementPMID:26582768
phenotype enhanced byhht2histone H3 h3.2 Phenotypic EnhancementPMID:26582768
synthetic lethal withabo2ATPase with bromodomain protein (predicted) Synthetic LethalityPMID:26582768
synthetic lethal withspt16FACT complex subunit Spt16 Synthetic LethalityPMID:26582768
synthetic growth defect withnhp6High-mobility group non-histone chromatin protein (predicted) Synthetic Growth DefectPMID:26582768
synthetic growth defect withtop2DNA topoisomerase II Synthetic Growth DefectPMID:25669599
External References
Database Identifier Description
NBRP SPAC31G5.19 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC31G5.19 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC31G5.19 BioGRID Interaction Datasets
Expression Viewer SPAC31G5.19 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC31G5.19 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC31G5.19 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC31G5.19 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC31G5.19 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC31G5.19 Transcriptome Viewer (Bähler Lab)
GEO SPAC31G5.19 GEO profiles
PInt SPAC31G5.19 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC31G5.19 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC31G5.19 Fission yeast phenotypic data & analysis
Cyclebase SPAC31G5.19.1 Cell Cycle Data
SPD / RIKEN48/48D10Orfeome Localization Data
UniProtKB/SwissProtO14114Uncharacterized AAA domain-containing protein C31G5.19
ModBaseO14114Database of comparative protein structure models
STRINGO14114Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594020ATPase with bromodomain protein (predicted)
RefSeq mRNANM_001019446972h- ATPase with bromodomain protein (predicted) (SPAC31G5.19), mRNA

Literature for abo1

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016