nde2 (SPAC3A11.07)


Gene Standard Namende2 Characterisation Statusbiological_role_inferred
Systematic IDSPAC3A11.07 Feature Typeprotein coding
Synonyms Name Description
Productmitochondrial NADH dehydrogenase (ubiquinone) Nde2 (predicted) Product Size551aa, 61.67 kDa
Genomic Location Chromosome I, 3456694-3459197 (2504nt); CDS:3457273-3458928 (1656nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
NADH dehydrogenase (ubiquinone) activity2
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mitochondrial ATP synthesis coupled electron transport24
Annotation ExtensionEvidenceWith/FromReference
NADH oxidation7
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
mitochondrial inner membrane163
Annotation ExtensionEvidenceWith/FromReference
mitochondrion761
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationnde2+ (wild type)Overexpression1428
viable vegetative cell populationnde2ΔNull3792

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologynde2ΔNull3092
Target Of
OntologyRelationshipGeneProductReference
GO regulated by sre1 sterol regulatory element binding protein, transcription factor Sre1 PMID:16537923
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3456694..3459197
mRNA3456694..3459197
5' UTR3456694..3457272PMID:21511999
CDS3457273..3458928
3' UTR3458929..3459197PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00070 Pfam IPR001327 Pyridine nucleotide-disulphide oxidoreductase, NAD-binding domain 256 343 6
PF07992 Pfam IPR023753 Pyridine nucleotide-disulphide oxidoreductase, FAD/NAD(P)-binding domain 94 411 9
PTHR22915 HMMPANTHER 37 549 2
PTHR22915:SF28 HMMPANTHER 37 549 2
3.50.50.60 Gene3D 89 221 29
3.50.50.60 Gene3D 256 432 29
SSF51905 SuperFamily 251 430 26
SSF51905 SuperFamily 92 307 26
PR00368 PRINTS IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase 388 410 7
PR00368 PRINTS IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase 195 213 7
PR00368 PRINTS IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase 256 274 7

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.93 Da
Charge 18.00
Isoelectric point 9.28
Molecular weight 61.67 kDa
Number of residues 551
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
250520during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
258734during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
246060during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
251239during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
46048.16during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
262620during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
47384.69during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
25during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byhhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withago1argonaute Positive GeneticPMID:22681890
negative genetic interaction withamk2AMP-activated protein kinase beta subunit Amk2 Negative GeneticPMID:22681890
positive genetic interaction withbzz1diacylglycerol binding protein Bzz1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
negative genetic interaction withgcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
positive genetic interaction withhrp3ATP-dependent DNA helicase Hrp3 Positive GeneticPMID:22681890
positive genetic interaction withiwr1RNA polymerase II nuclear import protein Iwr1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withknd1Cullin-associated NEDD8-dissociated protein Knd1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withngg1SAGA complex subunit Ngg1 Negative GeneticPMID:22681890
positive genetic interaction withnot3CCR4-Not complex subunit Not3/5 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withpar1protein phosphatase regulatory subunit Par1 Negative GeneticPMID:22681890
positive genetic interaction withppk16serine/threonine protein kinase Ppk16 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withrcd1RNA-binding protein, CCR4-NOT complex subunit Rcd1 Positive GeneticPMID:22681890
positive genetic interaction withrsc4RSC complex subunit Rsc4 Positive GeneticPMID:22681890
negative genetic interaction withrxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
negative genetic interaction withsdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Negative GeneticPMID:22681890
positive genetic interaction withSPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPBPB10D8.02carylsulfatase (predicted) Positive GeneticPMID:22681890
negative genetic interaction withsts5RNB-like protein Negative GeneticPMID:22681890
negative genetic interaction withtea5pseudokinase Tea5 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC3A11.07 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC3A11.07 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC3A11.07 BioGRID Interaction Datasets
Expression Viewer SPAC3A11.07 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC3A11.07 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC3A11.07 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC3A11.07 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC3A11.07 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC3A11.07 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC3A11.07 Cell Cycle Data
GEO SPAC3A11.07 GEO profiles
PInt SPAC3A11.07 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC3A11.07 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC3A11.07 Fission yeast phenotypic data & analysis
SPD / RIKEN34/34G01Orfeome Localization Data
UniProtKB/SwissProtO14121Probable NADH-ubiquinone oxidoreductase C3A11.07, mitochondrial
ModBaseO14121Database of comparative protein structure models
STRINGO14121Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594196NADH dehydrogenase (predicted)
RefSeq mRNANM_001019620972h- NADH dehydrogenase (predicted) (SPAC3A11.07), mRNA
European Nucleotide ArchiveCAB16382.1ENA Protein Mapping
UniParcUPI000006A719UniProt Archive

Literature for nde2

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015