nde2 (SPAC3A11.07)


Gene Standard Namende2 Characterisation Statusbiological_role_inferred
Systematic IDSPAC3A11.07 Feature Typeprotein coding
Synonyms Name Description
Productmitochondrial NADH dehydrogenase (ubiquinone) Nde2 (predicted) Product Size551aa, 61.67 kDa
Genomic Location Chromosome I, 3456694-3459197 (2504nt); CDS:3457273-3458928 (1656nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0008137NADH dehydrogenase (ubiquinone) activityISOSGD:S000004589GO_REF:00000242
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006116NADH oxidationISSUniProtKB:Q07500GO_REF:00000247
GO:0042775mitochondrial ATP synthesis coupled electron transportISOSGD:S000004589GO_REF:000002424
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005743mitochondrial inner membraneISOSGD:S000004589GO_REF:0000024158
GO:0005739mitochondrionIDAPMID:16823372758
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopynde2ΔNullPMID:204732893759
Microscopynde2ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopynde2ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
GO regulated by sre1 sterol regulatory element binding protein, transcription factor Sre1 PMID:16537923
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
134566943459197

UTRs

Region Coordinates Reference
five_prime_UTR3456694..3457272PMID:21511999
three_prime_UTR3458929..3459197PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00070 Pfam IPR001327 Pyridine nucleotide-disulphide oxidoreductase, NAD-binding domain 256 343 6
PF07992 Pfam IPR023753 Pyridine nucleotide-disulphide oxidoreductase, FAD/NAD(P)-binding domain 94 411 9
PTHR22915 HMMPANTHER 32 551 2
PTHR22915:SF4 HMMPANTHER 32 551 2
3.50.50.60 Gene3D 256 432 29
3.50.50.60 Gene3D 89 221 29
SSF51905 SuperFamily 251 430 26
SSF51905 SuperFamily 92 307 26
PR00368 PRINTS IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase 195 213 7
PR00368 PRINTS IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase 256 274 7
PR00368 PRINTS IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase 388 410 7

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.93 Da
Charge 18.00
Isoelectric point 9.28
Molecular weight 61.67 kDa
Number of residues 551
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
250520during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
258734during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
246060during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
251239during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
262620during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
46048.16during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
47384.69during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
25during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4600
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
bzz1diacylglycerol binding protein Bzz1 (predicted) Positive GeneticPMID:22681890
not3CCR4-Not complex subunit Not3/5 (predicted) Positive GeneticPMID:22681890
ppk16serine/threonine protein kinase Ppk16 (predicted) Positive GeneticPMID:22681890
par1protein phosphatase regulatory subunit Par1 Negative GeneticPMID:22681890
SPBPB10D8.02carylsulfatase (predicted) Positive GeneticPMID:22681890
rcd1RNA-binding protein, CCR4-NOT complex subunit Rcd1 Positive GeneticPMID:22681890
tea5pseudokinase Tea5 Negative GeneticPMID:22681890
rsc4RSC complex subunit Rsc4 Positive GeneticPMID:22681890
iwr1RNA polymerase II nuclear import protein Iwr1 (predicted) Positive GeneticPMID:22681890
ago1argonaute Positive GeneticPMID:22681890
knd1Cullin-associated NEDD8-dissociated protein Knd1 (predicted) Positive GeneticPMID:22681890
sts5RNB-like protein Negative GeneticPMID:22681890
rxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
hrp3ATP-dependent DNA helicase Hrp3 Positive GeneticPMID:22681890
SPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
sdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Negative GeneticPMID:22681890
ngg1SAGA complex subunit Ngg1 Negative GeneticPMID:22681890
amk2AMP-activated protein kinase beta subunit Amk2 Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPAC3A11.07 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC3A11.07 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC3A11.07 BioGRID Interaction Datasets
Expression Viewer SPAC3A11.07 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC3A11.07 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC3A11.07 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC3A11.07 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC3A11.07 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC3A11.07 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC3A11.07 Cell Cycle Data
GEO SPAC3A11.07 GEO profiles
PInt SPAC3A11.07 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC3A11.07 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC3A11.07 Fission yeast phenotypic data & analysis
SPD / RIKEN34/34G01Orfeome Localization Data
UniProtKB/SwissProtO14121Probable NADH-ubiquinone oxidoreductase C3A11.07, mitochondrial
ModBaseO14121Database of comparative protein structure models
STRINGO14121Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594196NADH dehydrogenase (predicted)
RefSeq mRNANM_001019620972h- NADH dehydrogenase (predicted) (SPAC3A11.07), mRNA
European Nucleotide ArchiveCAB16382.1ENA Protein Mapping
UniParcUPI000006A719UniProt Archive

Literature for nde2

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014