cwf2 (SPAC3A12.11c)


Gene Standard Namecwf2 Characterisation Statuspublished
Systematic IDSPAC3A12.11c Feature Typeprotein coding
Synonymscwc2, prp3 Name DescriptionComplexed With cdc Five
ProductRNA-binding protein Cwf2 Product Size388aa, 44.28 kDa
Genomic Location Chromosome I, 1440658-1439245 (1414nt); CDS:1440543-1439274 (1270nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
metal ion binding747
Annotation ExtensionEvidenceWith/FromReference
pre-mRNA binding3
Annotation ExtensionEvidenceWith/FromReference
protein binding831
Annotation ExtensionEvidenceWith/FromReference
U6 snRNA binding2
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mRNA cis splicing, via spliceosome116
Annotation ExtensionEvidenceWith/FromReference
positive regulation of cell cycle124
Annotation ExtensionEvidenceWith/FromReference
positive regulation of RNA splicing3
Annotation ExtensionEvidenceWith/FromReference
spliceosomal snRNP assembly11
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nucleus2740
Annotation ExtensionEvidenceWith/FromReference
Prp19 complex16
Annotation ExtensionEvidenceWith/FromReference
spliceosomal complex107
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopycwf2ΔNullPECO:0000005, PECO:0000137PMID:236978061338
Cell growth assaycwf2ΔNullPMID:10409726
Microscopycwf2ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopycwf2ΔNullPECO:0000005, PECO:0000137PMID:23697806686
FYPO:0000314inviable after spore germination with elongated germ tube163
penetrance FYPO_EXT:0000001Microscopycwf2ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
114406581440326
214402761440112
314400571439245

UTRs

Region Coordinates Reference
five_prime_UTR1440658..1440544PMID:21511999
three_prime_UTR1439273..1439245PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00076 Pfam IPR000504 RNA recognition motif domain 176 238 61
SM00360 SMART IPR000504 RNA recognition motif domain 175 244 68
SM00356 SMART IPR000571 Zinc finger, CCCH-type 111 137 8
PS50103 Prosite Profiles IPR000571 Zinc finger, CCCH-type 111 138 14
PS50102 Prosite Profiles IPR000504 RNA recognition motif domain 174 248 72
PTHR14089:SF2 HMMPANTHER 7 315 1
PTHR14089 HMMPANTHER 7 315 2
3.30.70.330 Gene3D IPR012677 Nucleotide-binding, alpha-beta plait 43 59 83
3.30.70.330 Gene3D IPR012677 Nucleotide-binding, alpha-beta plait 170 254 83
SSF54928 SuperFamily 164 255 78
SSF90229 SuperFamily 114 136 7

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000137zinc finger proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000137
PBO:0000302rrm RNA recognition motifTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000302
PBO:0000416zf-CCCH typeTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000416

Protein Properties

Ave. residue weight 114.13 Da
Charge -6.00
Isoelectric point 5.40
Molecular weight 44.28 kDa
Number of residues 388
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS334PMID:217125471670
present during mitotic M phase
present during mitotic M phaseexperimental evidenceS385PMID:21712547
present during mitotic M phaseexperimental evidenceS2PMID:21712547
present during cellular response to thiabendazoleIDAS385PMID:18257517
experimental evidenceS2PMID:24763107
MOD:00696phosphorylated residueNASPMID:182575171922
NASPMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4146during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3908during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3878during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3107during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
826.7during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
3345during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3745.06during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.8during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.69during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
tal1transaldolase (predicted) Affinity Capture-MSPMID:21386897
SPBC428.12cRNA-binding protein Affinity Capture-MSPMID:21386897
SPAC6G10.10chuman hmmtag2 homolog Affinity Capture-MSPMID:21386897
cwf18complexed with Cdc5 protein Cwf18 Affinity Capture-MSPMID:21386897
cwf26complexed with Cdc5 protein Cwf26 Affinity Capture-MSPMID:21386897
cct5chaperonin-containing T-complex epsilon subunit Cct5 Affinity Capture-MSPMID:21386897
SPAC24H6.10cphospho-2-dehydro-3-deoxyheptonate aldolase (predicted) Affinity Capture-MSPMID:21386897
red1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
syf2splicing factor Syf2 Affinity Capture-MSPMID:21386897
cwf2RNA-binding protein Cwf2 Affinity Capture-MSPMID:21386897
smd3Sm snRNP core protein Smd3 Affinity Capture-MSPMID:21386897
sap145U2 snRNP-associated protein Sap145 Affinity Capture-MSPMID:21386897
SPAC20H4.09ATP-dependent RNA helicase, spliceosomal (predicted) Affinity Capture-MSPMID:21386897
tif51translation elongation factor eIF5A (predicted) Affinity Capture-MSPMID:21386897
fps1geranyltranstransferase Fps1 Affinity Capture-MSPMID:21386897
dbp5cytoplasmic ATP-dependent RNA helicase Dbp5 (predicted) Affinity Capture-MSPMID:21386897
mtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
pdb1pyruvate dehydrogenase e1 component beta subunit Pdb1 Affinity Capture-MSPMID:21386897
cwf12complexed with Cdc5 protein Cwf12 Affinity Capture-MSPMID:21386897
SPAC25G10.01RNA-binding protein Affinity Capture-MSPMID:21386897
SPBC713.05WD repeat protein, human MAPK organizer 1 (MORG1) family (predicted) Affinity Capture-MSPMID:21386897
SPAC29A4.15cytoplasmic serine-tRNA ligase Srs1 (predicted) Affinity Capture-MSPMID:21386897
prp19ubiquitin-protein ligase E4 Affinity Capture-MSPMID:21386897
Two-hybridPMID:12088152
prp12U2 snRNP-associated protein Sap130 Affinity Capture-MSPMID:21386897
prp5WD repeat protein Prp5 Affinity Capture-MSPMID:21386897
sme1Sm snRNP core protein Sme1 Affinity Capture-MSPMID:21386897
cdc28ATP-dependent RNA helicase Cdc28 Affinity Capture-MSPMID:21386897
smg1Sm snRNP core protein Smg1 Affinity Capture-MSPMID:21386897
SPBC1703.07ATP citrate synthase subunit 1 (predicted) Affinity Capture-MSPMID:21386897
erg11sterol 14-demethylase Erg11 (predicted) Affinity Capture-MSPMID:21386897
saf2splicing associated factor Saf2 Affinity Capture-MSPMID:21386897
pil1eisosome BAR domain protein Pil1 Affinity Capture-MSPMID:21386897
cwf19complexed with Cdc5 protein Cwf19 Affinity Capture-MSPMID:21386897
cwf10U5 snRNP GTPase subunit Cwf10 Affinity Capture-MSPMID:21386897
Co-purificationPMID:24014766
Affinity Capture-WesternPMID:24442611
Affinity Capture-MS
sap49U2 snRNP-associated RNA-binding protein Sap49 Affinity Capture-MSPMID:21386897
gar1snoRNP pseudouridylase box H/ACA snoRNP complex protein Gar1 Affinity Capture-MSPMID:21386897
lea1U2 snRNP-associated protein Lea1 (predicted) Affinity Capture-MSPMID:21386897
Affinity Capture-MSPMID:24442611
Affinity Capture-Western
spf38U5 snRNP complex subunit Spf38 Affinity Capture-MSPMID:21386897
cwf4complexed with Cdc5 protein Cwf4 Affinity Capture-MSPMID:21386897
dre4splicing associated factor Dre4 Affinity Capture-MSPMID:21386897
Affinity Capture-Western
trp3anthranilate synthase component I (predicted) Affinity Capture-MSPMID:21386897
sap61U2 snRNP-associated protein sap61 Affinity Capture-MSPMID:21386897
prp16ATP-dependent RNA helicase Prp16 Affinity Capture-MSPMID:21386897
cwf3complexed with Cdc5 protein Cwf3 Affinity Capture-MSPMID:21386897
bpb1zinc finger splicing factor Bpb1 Affinity Capture-MSPMID:21386897
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
sap114U2 snRNP subunit Sap114 Affinity Capture-MSPMID:21386897
SPAC20H4.06cRNA-binding protein Affinity Capture-MSPMID:21386897
cwf25complexed with Cdc5 protein Cwf25 Affinity Capture-MSPMID:21386897
cwf5RNA-binding protein Cwf5 Affinity Capture-MSPMID:21386897
mug161CwfJ family protein, splicing factor (predicted) Affinity Capture-MSPMID:21386897
cwf7splicing factor Cwf7 Affinity Capture-MSPMID:21386897
int6eIF3e subunit Int6 Affinity Capture-MSPMID:21386897
cdc5splicing factor, Prp19 complex subunit Cdc5 Affinity Capture-MSPMID:21386897
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-MSPMID:10409726
Affinity Capture-Western
prp10U2 snRNP-associated protein Sap155 Affinity Capture-MSPMID:21386897
smb1Sm snRNP core protein Smb1 Affinity Capture-MSPMID:21386897
cwf15complexed with Cdc5 protein Cwf15 Affinity Capture-MSPMID:21386897
spi1Ran GTPase Spi1 Affinity Capture-MSPMID:21386897
rsd1RNA-binding protein Rsd1 (predicted) Affinity Capture-MSPMID:21386897
prp17splicing factor Prp17 Affinity Capture-MSPMID:21386897
sde2silencing defective protein Sde2 Affinity Capture-MSPMID:21386897
prp43ATP-dependent RNA helicase Prp43 Affinity Capture-MSPMID:21386897
prp45splicing factor Prp45 Affinity Capture-MSPMID:21386897
mug64BAR domain protein (predicted) Affinity Capture-MSPMID:21386897
cwf14G10 protein Affinity Capture-MSPMID:24874881
Affinity Capture-MSPMID:21386897
sap14U2 snRNP-associated protein SF3B14 Sap14 Affinity Capture-MSPMID:21386897
smf1Sm snRNP core protein Smf1 Affinity Capture-MSPMID:21386897
hxk1hexokinase 1 Affinity Capture-MSPMID:21386897
lat1dihydrolipoamide S-acetyltransferase E2, Lat1 (predicted) Affinity Capture-MSPMID:21386897
cct2chaperonin-containing T-complex beta subunit Cct2 Affinity Capture-MSPMID:21386897
ypt1GTPase Ypt1 Affinity Capture-MSPMID:21386897
prp22ATP-dependent RNA helicase Prp22 Affinity Capture-MSPMID:21386897
uap56ATP-dependent RNA helicase Uap56 Affinity Capture-MSPMID:21386897
qrs1cytoplasmic glutaminyl-tRNA ligase Qrs1 (predicted) Affinity Capture-MSPMID:21386897
luc7U1 snRNP-associated protein Luc7 Affinity Capture-MSPMID:21386897
bis1stress response protein Bis1 Affinity Capture-MSPMID:21386897
ini1RING finger-like protein Ini1 Affinity Capture-MSPMID:21386897
SPCC5E4.10chuman leukocyte receptor 1 ortholog Affinity Capture-MSPMID:21386897
cwf11complexed with Cdc5 protein Cwf11 Affinity Capture-MSPMID:21386897
SPAC1006.07translation initiation factor eIF4A (predicted) Affinity Capture-MSPMID:21386897
sap62U2 snRNP Sap62 Affinity Capture-MSPMID:21386897
pda1pyruvate dehydrogenase e1 component alpha subunit Pda1 (predicted) Affinity Capture-MSPMID:21386897
prp2U2AF large subunit (U2AF-59) Affinity Capture-MSPMID:21386897
rfp1SUMO-targeted ubiquitin-protein ligase subunit Rfp1 Affinity Capture-MSPMID:21386897
ade3phosphoribosylformylglycinamidine synthase Ade3 (predicted) Affinity Capture-MSPMID:21386897
smd1Sm snRNP core protein Smd1 Affinity Capture-MSPMID:21386897
eca39branched chain amino acid aminotransferase Eca39 Affinity Capture-MSPMID:21386897
cyp1cyclophilin family peptidyl-prolyl cis-trans isomerase Cyp1 Affinity Capture-MSPMID:21386897
cwf21complexed with Cdc5 protein Cwf21 Affinity Capture-MSPMID:21386897
SPAC22A12.16ATP-citrate synthase subunit 2 (predicted) Affinity Capture-MSPMID:21386897
SPAC1486.03cRNA-binding splicing factor (predicted) Affinity Capture-MSPMID:21386897
pir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
SPAC1F12.07phosphoserine aminotransferase (predicted) Affinity Capture-MSPMID:21386897
spp42U5 snRNP complex subunit Spp42 Affinity Capture-MSPMID:21386897
met16phosphoadenosine phosphosulfate reductase Affinity Capture-MSPMID:21386897
brr2U5 snRNP complex subunit Brr2 Affinity Capture-MSPMID:21386897
mpg2mannose-1-phosphate guanyltransferase (predicted) Affinity Capture-MSPMID:21386897
msl1U2 snRNP-associated protein Msl1 (predicted) Affinity Capture-MSPMID:21386897
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
trs8502TRAPP complex subunit Trs85b (predicted) Negative GeneticPMID:22681890
zrg17cation diffusion family zinc membrane transporter Zrg17 Negative GeneticPMID:22681890
mms1E3 ubiquitin ligase complex subunit Mms1 (predicted) Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC3A12.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC3A12.11c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC3A12.11c BioGRID Interaction Datasets
Expression Viewer SPAC3A12.11c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC3A12.11c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC3A12.11c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC3A12.11c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC3A12.11c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC3A12.11c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC3A12.11c Cell Cycle Data
GEO SPAC3A12.11c GEO profiles
PInt SPAC3A12.11c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC3A12.11c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC3A12.11c Fission yeast phenotypic data & analysis
SPD / RIKEN20/20C12Orfeome Localization Data
UniProtKB/SwissProtP87126Pre-mRNA-splicing factor cwf2
ModBaseP87126Database of comparative protein structure models
STRINGP87126Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593337RNA-binding protein Cwf2
RefSeq mRNANM_001018769972h- RNA-binding protein Cwf2 (cwf2), mRNA
European Nucleotide ArchiveAAF63625.1ENA Protein Mapping
European Nucleotide ArchiveCAC41387.1ENA Protein Mapping
UniParcUPI0000128732UniProt Archive

Literature for cwf2

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014