cwf2 (SPAC3A12.11c)

Gene Standard Namecwf2 ChromosomeI
Systematic IDSPAC3A12.11c Gene Start1440658
Synonymscwc2, prp3 Gene End1439245
ProductRNA-binding protein Cwf2 Gene Length1414
Feature Typeprotein coding CDS Start1440543
Name Descriptioncomplexed with Cdc5 CDS End1439274
Characterisation Statuspublished CDS Length1270

Ensembl Gene Location
  1. SPAC3A12.06c
  2. SPAC3A12.19
  3. rpb11
  4. SPAC3A12.08
  5. SPAC3A12.09c
  6. SPNCRNA.741
  7. rpl2001
  8. cwf2
  9. atp11
  10. SPAC3A12.13c
  11. cam1
  12. vps53
Fission Yeast Phenotype Ontology

Ontology Graph

Contact Curators
Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0002061inviable vegetative cell populationMicroscopycwf2delta (deletion)deletionPMID:20473289
GO Molecular Function

Ontology Graph

Contact Curators
Term IDTerm NameEvidenceWith/FromReference
GO:0005515protein bindingIPIprp19PMID:12088152
IPIcdc5PMID:12088152
GO:0008270zinc ion bindingIEAIPR000571GO_REF:0000002
GO:0017070U6 snRNA bindingISSUniProtKB:Q12046GO_REF:0000024
GO:0036002pre-mRNA bindingISSUniProtKB:Q12046GO_REF:0000024
GO Biological Process

Ontology Graph

Contact Curators
Term IDTerm NameEvidenceWith/FromReference
GO:0000387spliceosomal snRNP assemblyISSUniProtKB:Q12046GO_REF:0000024
GO:0033120positive regulation of RNA splicingISSUniProtKB:Q12046GO_REF:0000024
GO:0045292mRNA cis splicing, via spliceosomeISSUniProtKB:Q12046GO_REF:0000024
requiredIMPGO:0005681PMID:12088152
GO:0045787positive regulation of cell cycleISSUniProtKB:Q12046GO_REF:0000024
GO Cellular Component

Ontology Graph

Contact Curators
Term IDTerm NameEvidenceWith/FromReference
GO:0000974Prp19 complexISSUniProtKB:Q12046GO_REF:0000024
GO:0005634nucleusIDAPMID:16823372
GO:0005681spliceosomal complexIDAPMID:12088152
Transcript
Contact Curators
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
114406581440326
214402761440112
314400571439245

References

Region Start End Reference
five_prime_UTR14406581440544PMID:21511999
three_prime_UTR14392731439245PMID:21511999
three_prime_UTR14392731439245PMID:21511999
Protein Features
Contact Curators

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF00076 IPR000504 Pfam RNA recognition motif domain 176 238 61
SM00356 IPR000571 SMART Zinc finger, CCCH-type 111 137 8
SM00360 IPR000504 SMART RNA recognition motif domain 175 244 69
PS50103 IPR000571 Prosite Profiles Zinc finger, CCCH-type 111 138 14
PS50102 IPR000504 Prosite Profiles RNA recognition motif domain 174 248 72
G3DSA:3.30.70.330 IPR012677 Gene3D 43 59 82
G3DSA:3.30.70.330 IPR012677 Gene3D 170 254 82
SSF54928 SuperFamily 164 255 78
SSF90229 SuperFamily 114 136 7
PTHR14089 hmmpanther 38 370 2
PTHR14089:SF2 hmmpanther 38 370 1

View domain organization at Pfam

Protein Modifications (PSI-MOD)

Term IDTerm NameEvidenceResidueReference
MOD:00696phosphorylated residuePMID:18257517
PMID:19547744

Manually Curated Family or Sub-families

Term IDTerm NameReferenceAll Genes
PBO:0000137zinc finger proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000137
PBO:0000302rrm RNA recognition motifTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000302
PBO:0000416zf-CCCH typeTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000416

Protein Properties

Ave. residue weight 114.13 Da
Charge -6.00
Isoelectric point 5.40
Molecular weight 44.28 kDa
Number of residues 388
Sequence
Contact Curators
Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide3745.06PMID:23101633
experimental evidencePECO:0000014,
PECO:0000005		population_wide826.7PMID:23101633
RNA levelexperimental evidencePECO:0000014,
PECO:0000005		population_wide2.8PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide0.69PMID:23101633
Species Distribution
DescriptionQualifierReference
predominantly single copy (one to one)
conserved in fungi
conserved in eukaryotes
conserved in metazoa
conserved in vertebrates
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

Contact Curators
Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
zrg17cation diffusion family zinc membrane transporter Zrg17 Negative GeneticPMID:22681890
SPBC1734.07cTRAPP complex subunit Trs85 (predicted) Negative GeneticPMID:22681890
mms1E3 ubiquitin ligase complex subunit Mms1 (predicted) Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
prp19ubiquitin-protein ligase E4 Two-hybridPMID:12088152
Affinity Capture-MSPMID:21386897
cwf21complexed with Cdc5 protein Cwf21 Affinity Capture-MSPMID:21386897
sap62U2 snRNP Sap62 Affinity Capture-MSPMID:21386897
syf2splicing factor Syf2 Affinity Capture-MSPMID:21386897
cwf3complexed with Cdc5 protein Cwf3 Affinity Capture-MSPMID:21386897
pil1eisosome BAR domain protein Pil1 Affinity Capture-MSPMID:21386897
qrs1cytoplasmic glutaminyl-tRNA ligase Qrs1 (predicted) Affinity Capture-MSPMID:21386897
dre4splicing associated factor Dre4 Affinity Capture-WesternPMID:21386897
Affinity Capture-MS
cwf25complexed with Cdc5 protein Cwf25 Affinity Capture-MSPMID:21386897
trp3anthranilate synthase component I (predicted) Affinity Capture-MSPMID:21386897
cwf10U5 snRNP GTPase subunit Cwf10 Affinity Capture-MSPMID:21386897
SPBC713.05WD repeat protein, human MAPK organizer 1 (MORG1) family (predicted) Affinity Capture-MSPMID:21386897
mug64BAR domain protein (predicted) Affinity Capture-MSPMID:21386897
SPCC5E4.10chuman leukocyte receptor 1 ortholog Affinity Capture-MSPMID:21386897
spf38U5 snRNP complex subunit Spf38 Affinity Capture-MSPMID:21386897
cwf26complexed with Cdc5 protein Cwf26 Affinity Capture-MSPMID:21386897
pdb1pyruvate dehydrogenase e1 component beta subunit Pdb1 Affinity Capture-MSPMID:21386897
tal1transaldolase (predicted) Affinity Capture-MSPMID:21386897
SPAC1006.07translation initiation factor eIF4A (predicted) Affinity Capture-MSPMID:21386897
cct2chaperonin-containing T-complex beta subunit Cct2 Affinity Capture-MSPMID:21386897
prp16ATP-dependent RNA helicase Prp16 Affinity Capture-MSPMID:21386897
tif51translation elongation factor eIF5A (predicted) Affinity Capture-MSPMID:21386897
SPAC24H6.10cphospho-2-dehydro-3-deoxyheptonate aldolase (predicted) Affinity Capture-MSPMID:21386897
cdc5cell division control protein, splicing factor Cdc5 Affinity Capture-MSPMID:15755920
Affinity Capture-MSPMID:10409726
Affinity Capture-Western
Affinity Capture-MSPMID:21386897
lat1dihydrolipoamide S-acetyltransferase E2, Lat1 (predicted) Affinity Capture-MSPMID:21386897
prp2U2AF large subunit (U2AF-59) Affinity Capture-MSPMID:21386897
SPBC1703.07ATP citrate synthase subunit 1 (predicted) Affinity Capture-MSPMID:21386897
eca39branched chain amino acid aminotransferase Eca39 Affinity Capture-MSPMID:21386897
cwf7splicing factor Cwf7 Affinity Capture-MSPMID:21386897
prp22ATP-dependent RNA helicase Prp22 Affinity Capture-MSPMID:21386897
sde2silencing defective protein Sde2 Affinity Capture-MSPMID:21386897
SPAC6G10.10chuman hmmtag2 homolog Affinity Capture-MSPMID:21386897
smg1snRNP core protein Smg1 Affinity Capture-MSPMID:21386897
lea1U2 snRNP-associated protein Lea1 (predicted) Affinity Capture-MSPMID:21386897
SPAC20H4.09ATP-dependent RNA helicase, spliceosomal (predicted) Affinity Capture-MSPMID:21386897
sap114U2 snRNP subunit Sap114 Affinity Capture-MSPMID:21386897
sme1Sm snRNP core protein Sme1 Affinity Capture-MSPMID:21386897
cct5chaperonin-containing T-complex epsilon subunit Cct5 Affinity Capture-MSPMID:21386897
hxk1hexokinase 1 Affinity Capture-MSPMID:21386897
cwf14G10 protein Affinity Capture-MSPMID:21386897
cwf15complexed with Cdc5 protein Cwf15 Affinity Capture-MSPMID:21386897
prp12U2 snRNP-associated protein Sap130 Affinity Capture-MSPMID:21386897
prp5WD repeat protein Prp5 Affinity Capture-MSPMID:21386897
ade3phosphoribosylformylglycinamidine synthase Ade3 (predicted) Affinity Capture-MSPMID:21386897
ini1RING finger-like protein Ini1 Affinity Capture-MSPMID:21386897
met16phosphoadenosine phosphosulfate reductase Affinity Capture-MSPMID:21386897
mpg2mannose-1-phosphate guanyltransferase (predicted) Affinity Capture-MSPMID:21386897
cwf5RNA-binding protein Cwf5 Affinity Capture-MSPMID:21386897
SPAC20H4.06cRNA-binding protein Affinity Capture-MSPMID:21386897
msl1U2 snRNP-associated protein Msl1 (predicted) Affinity Capture-MSPMID:21386897
cdc28ATP-dependent RNA helicase Cdc28 Affinity Capture-MSPMID:21386897
smd1Sm snRNP core protein Smd1 Affinity Capture-MSPMID:21386897
sap49U2 snRNP-associated RNA-binding protein Sap49 Affinity Capture-MSPMID:21386897
bis1stress response protein Bis1 Affinity Capture-MSPMID:21386897
spi1Ran GTPase Spi1 Affinity Capture-MSPMID:21386897
gar1snoRNP pseudouridylase box H/ACA snoRNP complex protein Gar1 Affinity Capture-MSPMID:21386897
smd3Sm snRNP core protein Smd3 Affinity Capture-MSPMID:21386897
erg11sterol 14-demethylase (predicted) Affinity Capture-MSPMID:21386897
int6eIF3e subunit Int6 Affinity Capture-MSPMID:21386897
sap14U2 snRNP-associated protein SF3B14 Sap14 Affinity Capture-MSPMID:21386897
cwf11complexed with Cdc5 protein Cwf11 Affinity Capture-MSPMID:21386897
SPBC428.12cRNA-binding protein Affinity Capture-MSPMID:21386897
cwf19complexed with Cdc5 protein Cwf19 Affinity Capture-MSPMID:21386897
mug161CwfJ family protein, splicing factor (predicted) Affinity Capture-MSPMID:21386897
SPAC25G10.01RNA-binding protein Affinity Capture-MSPMID:21386897
rsd1RNA-binding protein Rsd1 (predicted) Affinity Capture-MSPMID:21386897
sap145U2 snRNP-associated protein Sap145 Affinity Capture-MSPMID:21386897
prp17splicing factor Prp17 Affinity Capture-MSPMID:21386897
SPAC29A4.15cytoplasmic serine-tRNA ligase Srs1 (predicted) Affinity Capture-MSPMID:21386897
cwf12complexed with Cdc5 protein Cwf12 Affinity Capture-MSPMID:21386897
bpb1zinc finger splicing factor Bpb1 Affinity Capture-MSPMID:21386897
ypt1GTPase Ypt1 Affinity Capture-MSPMID:21386897
cyp1cyclophilin family peptidyl-prolyl cis-trans isomerase Cyp1 Affinity Capture-MSPMID:21386897
SPAC22A12.16ATP-citrate synthase subunit 2 (predicted) Affinity Capture-MSPMID:21386897
sap61U2 snRNP-associated protein sap61 Affinity Capture-MSPMID:21386897
cwf4complexed with Cdc5 protein Cwf4 Affinity Capture-MSPMID:21386897
saf2splicing associated factor Saf2 Affinity Capture-MSPMID:21386897
uap56ATP-dependent RNA helicase Uap56 Affinity Capture-MSPMID:21386897
pda1pyruvate dehydrogenase e1 component alpha subunit Pda1 (predicted) Affinity Capture-MSPMID:21386897
smb1Sm snRNP core protein Smb1 Affinity Capture-MSPMID:21386897
SPBC12C2.06cytoplasmic ATP-dependent RNA helicase Dbp5 (predicted) Affinity Capture-MSPMID:21386897
fps1geranyltranstransferase Fps1 Affinity Capture-MSPMID:21386897
brr2U5 snRNP complex subunit Brr2 Affinity Capture-MSPMID:21386897
spp42U5 snRNP complex subunit Spp42 Affinity Capture-MSPMID:21386897
prp43ATP-dependent RNA helicase Prp43 Affinity Capture-MSPMID:21386897
SPAC1486.03cRNA-binding splicing factor (predicted) Affinity Capture-MSPMID:21386897
prp10U2 snRNP-associated protein Sap155 Affinity Capture-MSPMID:21386897
cwf18complexed with Cdc5 protein Cwf18 Affinity Capture-MSPMID:21386897
SPAC1F12.07phosphoserine aminotransferase (predicted) Affinity Capture-MSPMID:21386897
smf1Sm snRNP core protein Smf1 Affinity Capture-MSPMID:21386897
rfp1SUMO-targeted ubiquitin-protein ligase subunit Rfp1 Affinity Capture-MSPMID:21386897
prp45splicing factor Prp45 Affinity Capture-MSPMID:21386897
luc7U1 snRNP-associated protein Luc7 Affinity Capture-MSPMID:21386897
Contact Curators
External References
Database Identifier Description
NBRP SPAC3A12.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC3A12.11c Retrieval of eukaryotic orthologs
BioGrid SPAC3A12.11c BioGRID Interaction Datasets
Bähler Lab SPAC3A12.11c Cell Cycle Expression Profile
Bähler Lab SPAC3A12.11c Meiosis/Sporulation Expression Profies
Bähler Lab SPAC3A12.11c Pheromone response/mating expression profiles
Bähler Lab SPAC3A12.11c Environmental stress expression profiles
Bähler Lab SPAC3A12.11c Bähler Lab Transcriptome Viewer
Cyclebase SPAC3A12.11c Cell Cycle Data
PInt SPAC3A12.11c Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPAC3A12.11c Entrez Gene
WikiGene2542531RNA-binding protein Cwf2
Uniprot_genenameCWF2
EntrezGene2542531RNA-binding protein Cwf2
UniProtKB/SwissProtP87126Pre-mRNA-splicing factor cwf2
ModBaseP87126Database of comparative protein structure models
Pfam Protein DomainsP87126Pfam Domain Arrangement
RefSeq PeptideNP_593337RNA-binding protein Cwf2
RefSeq mRNANM_001018769972h- RNA-binding protein Cwf2 (cwf2), mRNA
European Nucleotide ArchiveAAF63625ENA Protein Mapping
European Nucleotide ArchiveCAC41387ENA Protein Mapping
SPD / RIKEN20/20C12Orfeome Localization Data
UniParcUPI0000128732UniProt Archive

Literature for cwf2
Contact Curators

Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013