cwf2 (SPAC3A12.11c)


Gene Standard Namecwf2 Characterisation Statuspublished
Systematic IDSPAC3A12.11c Feature Typeprotein coding
Synonymscwc2, prp3 Name DescriptionComplexed With cdc Five
ProductRNA-binding protein Cwf2 Product Size388aa, 44.28 kDa
Genomic Location Chromosome I, 1440658-1439245 (1414nt); CDS:1440543-1439274 (1270nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
metal ion binding746
Annotation ExtensionEvidenceWith/FromReference
pre-mRNA binding3
Annotation ExtensionEvidenceWith/FromReference
protein binding851
Annotation ExtensionEvidenceWith/FromReference
U6 snRNA binding2
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mRNA cis splicing, via spliceosome118
Annotation ExtensionEvidenceWith/FromReference
positive regulation of RNA splicing4
Annotation ExtensionEvidenceWith/FromReference
spliceosomal snRNP assembly14
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nucleus2697
Annotation ExtensionEvidenceWith/FromReference
Prp19 complex16
Annotation ExtensionEvidenceWith/FromReference
spliceosomal complex108
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationcwf2ΔNull1428

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic cell cyclecwf2ΔNull808
inviable after spore germination with elongated germ tubecwf2ΔNull163
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
114406581440326
214402761440112
314400571439245

UTRs

Region Coordinates Reference
five_prime_UTR1440658..1440544PMID:21511999
three_prime_UTR1439273..1439245PMID:21511999
exon1440543..1440326,1440276..1440112,1440057..1439274
intron1440325..1440277
mRNA1440658..1439245
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00076 Pfam IPR000504 RNA recognition motif domain 176 238 61
SM00356 SMART IPR000571 Zinc finger, CCCH-type 111 137 8
SM00360 SMART IPR000504 RNA recognition motif domain 175 244 68
PS50102 Prosite Profiles IPR000504 RNA recognition motif domain 174 248 72
PS50103 Prosite Profiles IPR000571 Zinc finger, CCCH-type 111 138 14
PTHR14089 HMMPANTHER 41 324 4
PTHR14089:SF2 HMMPANTHER 41 324 1
3.30.70.330 Gene3D IPR012677 Nucleotide-binding alpha-beta plait domain 43 59 83
3.30.70.330 Gene3D IPR012677 Nucleotide-binding alpha-beta plait domain 170 254 83
SSF90229 SuperFamily 114 136 7
SSF54928 SuperFamily 164 255 78

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000137zinc finger proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000137
PBO:0000302rrm RNA recognition motifTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000302
PBO:0000416zf-CCCH typeTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000416

Protein Properties

Ave. residue weight 114.13 Da
Charge -6.00
Isoelectric point 5.40
Molecular weight 44.28 kDa
Number of residues 388
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS2 1670
present during cellular response to thiabendazole, mitotic M phaseS385
present during mitotic M phaseS334
present during mitotic M phaseS2
Annotation ExtensionEvidenceResidueReference
experimental evidence S2 PMID:24763107
present during mitotic M phase experimental evidence S2 PMID:21712547
present during mitotic M phase experimental evidence S334 PMID:21712547
present during mitotic M phase experimental evidence S385 PMID:21712547
present during cellular response to thiabendazole IDA S385 PMID:18257517
phosphorylated residue 1923
Annotation ExtensionEvidenceResidueReference
NAS PMID:18257517
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4146during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3908during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3878during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3107during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
826.7during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
3345during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3745.06during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.8during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.69during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesade3phosphoribosylformylglycinamidine synthase Ade3 (predicted) Affinity Capture-MSPMID:21386897
affinity capturesbis1stress response protein Bis1 Affinity Capture-MSPMID:21386897
affinity capturesbpb1zinc finger splicing factor Bpb1 Affinity Capture-MSPMID:21386897
affinity capturesbrr2U5 snRNP complex subunit Brr2 Affinity Capture-MSPMID:21386897
affinity capturescct2chaperonin-containing T-complex beta subunit Cct2 Affinity Capture-MSPMID:21386897
affinity capturescct5chaperonin-containing T-complex epsilon subunit Cct5 Affinity Capture-MSPMID:21386897
affinity capturescdc28ATP-dependent RNA helicase Cdc28 Affinity Capture-MSPMID:21386897
affinity capturescdc5splicing factor, Prp19 complex subunit Cdc5 Affinity Capture-MSPMID:21386897
co-purifies withcdc5splicing factor, Prp19 complex subunit Cdc5 Co-purificationPMID:24014766
affinity captured bycdc5splicing factor, Prp19 complex subunit Cdc5 Affinity Capture-MSPMID:15755920
affinity capturescdc5splicing factor, Prp19 complex subunit Cdc5 Affinity Capture-WesternPMID:10409726
affinity captured bycdc5splicing factor, Prp19 complex subunit Cdc5 Affinity Capture-MSPMID:10409726
affinity captured bycwf10U5 snRNP GTPase subunit Cwf10 Affinity Capture-WesternPMID:24442611
affinity captured bycwf10U5 snRNP GTPase subunit Cwf10 Affinity Capture-MSPMID:24442611
co-purifies withcwf10U5 snRNP GTPase subunit Cwf10 Co-purificationPMID:24014766
affinity capturescwf10U5 snRNP GTPase subunit Cwf10 Affinity Capture-MSPMID:21386897
affinity capturescwf11complexed with Cdc5 protein Cwf11 Affinity Capture-MSPMID:21386897
affinity capturescwf12complexed with Cdc5 protein Cwf12 Affinity Capture-MSPMID:21386897
affinity captured bycwf14G10 protein Affinity Capture-MSPMID:24874881
affinity capturescwf14G10 protein Affinity Capture-MSPMID:21386897
affinity capturescwf15complexed with Cdc5 protein Cwf15 Affinity Capture-MSPMID:21386897
affinity capturescwf18complexed with Cdc5 protein Cwf18 Affinity Capture-MSPMID:21386897
affinity capturescwf19complexed with Cdc5 protein Cwf19 Affinity Capture-MSPMID:21386897
affinity capturescwf2RNA-binding protein Cwf2 Affinity Capture-MSPMID:21386897
affinity capturescwf21complexed with Cdc5 protein Cwf21 Affinity Capture-MSPMID:21386897
affinity capturescwf25complexed with Cdc5 protein Cwf25 Affinity Capture-MSPMID:21386897
affinity capturescwf26complexed with Cdc5 protein Cwf26 Affinity Capture-MSPMID:21386897
affinity capturescwf3complexed with Cdc5 protein Cwf3 Affinity Capture-MSPMID:21386897
affinity capturescwf4complexed with Cdc5 protein Cwf4 Affinity Capture-MSPMID:21386897
affinity capturescwf5RNA-binding protein Cwf5 Affinity Capture-MSPMID:21386897
affinity capturescwf7splicing factor Cwf7 Affinity Capture-MSPMID:21386897
affinity capturescyp1cyclophilin family peptidyl-prolyl cis-trans isomerase Cyp1 Affinity Capture-MSPMID:21386897
affinity capturesdbp5cytoplasmic ATP-dependent RNA helicase Dbp5 (predicted) Affinity Capture-MSPMID:21386897
affinity captured bydre4splicing associated factor Dre4 Affinity Capture-MSPMID:21386897
affinity captured bydre4splicing associated factor Dre4 Affinity Capture-WesternPMID:21386897
affinity captureseca39branched chain amino acid aminotransferase Eca39 Affinity Capture-MSPMID:21386897
affinity captureserg11sterol 14-demethylase Erg11 (predicted) Affinity Capture-MSPMID:21386897
affinity capturesfps1geranyltranstransferase Fps1 Affinity Capture-MSPMID:21386897
affinity capturesgar1snoRNP pseudouridylase box H/ACA snoRNP complex protein Gar1 Affinity Capture-MSPMID:21386897
affinity captured byhhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
affinity captureshxk1hexokinase 1 Affinity Capture-MSPMID:21386897
affinity capturesini1RING finger-like protein Ini1 Affinity Capture-MSPMID:21386897
affinity capturesint6eIF3e subunit Int6 Affinity Capture-MSPMID:21386897
affinity captureslat1dihydrolipoamide S-acetyltransferase E2, Lat1 (predicted) Affinity Capture-MSPMID:21386897
affinity captured bylea1U2 snRNP-associated protein Lea1 (predicted) Affinity Capture-WesternPMID:24442611
affinity captured bylea1U2 snRNP-associated protein Lea1 (predicted) Affinity Capture-MSPMID:24442611
affinity captureslea1U2 snRNP-associated protein Lea1 (predicted) Affinity Capture-MSPMID:21386897
affinity capturesluc7U1 snRNP-associated protein Luc7 Affinity Capture-MSPMID:21386897
affinity capturesmet16phosphoadenosine phosphosulfate reductase Affinity Capture-MSPMID:21386897
affinity capturesmpg2mannose-1-phosphate guanyltransferase (predicted) Affinity Capture-MSPMID:21386897
affinity capturesmsl1U2 snRNP-associated protein Msl1 (predicted) Affinity Capture-MSPMID:21386897
affinity captured bymtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
affinity capturesmug161CwfJ family protein, splicing factor (predicted) Affinity Capture-MSPMID:21386897
affinity capturesmug64BAR domain protein (predicted) Affinity Capture-MSPMID:21386897
affinity capturespda1pyruvate dehydrogenase e1 component alpha subunit Pda1 (predicted) Affinity Capture-MSPMID:21386897
affinity capturespdb1pyruvate dehydrogenase e1 component beta subunit Pdb1 Affinity Capture-MSPMID:21386897
affinity capturespil1eisosome BAR domain protein Pil1 Affinity Capture-MSPMID:21386897
affinity captured bypir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
affinity capturesprp10U2 snRNP-associated protein Sap155 Affinity Capture-MSPMID:21386897
affinity capturesprp12U2 snRNP-associated protein Sap130 Affinity Capture-MSPMID:21386897
affinity capturesprp16ATP-dependent RNA helicase Prp16 Affinity Capture-MSPMID:21386897
affinity capturesprp17splicing factor Prp17 Affinity Capture-MSPMID:21386897
affinity captured byprp17splicing factor Prp17 Affinity Capture-MSPMID:21386897
affinity capturesprp19ubiquitin-protein ligase E4 Prp19 Affinity Capture-MSPMID:21386897
affinity captured byprp19ubiquitin-protein ligase E4 Prp19 Affinity Capture-MSPMID:21386897
binds activation domain construct withprp19ubiquitin-protein ligase E4 Prp19 Two-hybridPMID:12088152
affinity capturesprp2U2AF large subunit (U2AF-59) Affinity Capture-MSPMID:21386897
affinity capturesprp22ATP-dependent RNA helicase Prp22 Affinity Capture-MSPMID:21386897
affinity capturesprp43ATP-dependent RNA helicase Prp43 Affinity Capture-MSPMID:21386897
affinity capturesprp45splicing factor Prp45 Affinity Capture-MSPMID:21386897
affinity capturesprp5WD repeat protein Prp5 Affinity Capture-MSPMID:21386897
affinity capturesqrs1cytoplasmic glutaminyl-tRNA ligase Qrs1 (predicted) Affinity Capture-MSPMID:21386897
affinity captured byred1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
affinity capturesrfp1SUMO-targeted ubiquitin-protein ligase subunit Rfp1 Affinity Capture-MSPMID:21386897
affinity capturesrsd1RNA-binding protein Rsd1 (predicted) Affinity Capture-MSPMID:21386897
affinity captured bysaf2splicing associated factor Saf2 Affinity Capture-MSPMID:21386897
affinity capturessap114U2 snRNP subunit Sap114 Affinity Capture-MSPMID:21386897
affinity capturessap14U2 snRNP-associated protein SF3B14 Sap14 Affinity Capture-MSPMID:21386897
affinity capturessap145U2 snRNP-associated protein Sap145 Affinity Capture-MSPMID:21386897
affinity capturessap49U2 snRNP-associated RNA-binding protein Sap49 Affinity Capture-MSPMID:21386897
affinity capturessap61U2 snRNP-associated protein sap61 Affinity Capture-MSPMID:21386897
affinity capturessap62U2 snRNP Sap62 Affinity Capture-MSPMID:21386897
affinity capturessde2silencing defective protein Sde2 Affinity Capture-MSPMID:21386897
affinity captured bysde2silencing defective protein Sde2 Affinity Capture-MSPMID:25274039
affinity capturessmb1Sm snRNP core protein Smb1 Affinity Capture-MSPMID:21386897
affinity capturessmd1Sm snRNP core protein Smd1 Affinity Capture-MSPMID:21386897
affinity capturessmd3Sm snRNP core protein Smd3 Affinity Capture-MSPMID:21386897
affinity capturessme1Sm snRNP core protein Sme1 Affinity Capture-MSPMID:21386897
affinity capturessmf1Sm snRNP core protein Smf1 Affinity Capture-MSPMID:21386897
affinity capturessmg1Sm snRNP core protein Smg1 Affinity Capture-MSPMID:21386897
affinity capturesSPAC1006.07translation initiation factor eIF4A (predicted) Affinity Capture-MSPMID:21386897
affinity capturesSPAC1486.03cRNA-binding splicing factor (predicted) Affinity Capture-MSPMID:21386897
affinity capturesSPAC1F12.07phosphoserine aminotransferase (predicted) Affinity Capture-MSPMID:21386897
affinity capturesSPAC20H4.06cRNA-binding protein Affinity Capture-MSPMID:21386897
affinity capturesSPAC20H4.09ATP-dependent RNA helicase, spliceosomal (predicted) Affinity Capture-MSPMID:21386897
affinity capturesSPAC22A12.16ATP-citrate synthase subunit 2 (predicted) Affinity Capture-MSPMID:21386897
affinity capturesSPAC24H6.10cphospho-2-dehydro-3-deoxyheptonate aldolase (predicted) Affinity Capture-MSPMID:21386897
affinity capturesSPAC25G10.01RNA-binding protein Affinity Capture-MSPMID:21386897
affinity capturesSPAC6G10.10chuman hmmtag2 homolog Affinity Capture-MSPMID:21386897
affinity capturesSPBC1703.07ATP citrate synthase subunit 1 (predicted) Affinity Capture-MSPMID:21386897
affinity capturesSPBC428.12cRNA-binding protein Affinity Capture-MSPMID:21386897
affinity capturesSPBC713.05WD repeat protein, human MAPK organizer 1 (MORG1) family (predicted) Affinity Capture-MSPMID:21386897
affinity capturesSPCC5E4.10chuman leukocyte receptor 1 ortholog Affinity Capture-MSPMID:21386897
affinity capturesspf38U5 snRNP complex subunit Spf38 Affinity Capture-MSPMID:21386897
affinity capturesspi1Ran GTPase Spi1 Affinity Capture-MSPMID:21386897
affinity capturesspp42U5 snRNP complex subunit Spp42 Affinity Capture-MSPMID:21386897
affinity capturessrs1cytoplasmic serine-tRNA ligase Srs1 (predicted) Affinity Capture-MSPMID:21386897
affinity capturessyf2splicing factor Syf2 Affinity Capture-MSPMID:21386897
affinity capturestal1transaldolase (predicted) Affinity Capture-MSPMID:21386897
affinity capturestif51translation elongation factor eIF5A (predicted) Affinity Capture-MSPMID:21386897
affinity capturestrp3anthranilate synthase component I (predicted) Affinity Capture-MSPMID:21386897
affinity capturesuap56ATP-dependent RNA helicase Uap56 Affinity Capture-MSPMID:21386897
affinity capturesypt1GTPase Ypt1 Affinity Capture-MSPMID:21386897
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withmms1E3 ubiquitin ligase complex subunit Mms1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withtrs8502TRAPP complex subunit Trs85b (predicted) Negative GeneticPMID:22681890
negative genetic interaction withzrg17cation diffusion family zinc transmembrane transporter Zrg17 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC3A12.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC3A12.11c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC3A12.11c BioGRID Interaction Datasets
Expression Viewer SPAC3A12.11c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC3A12.11c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC3A12.11c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC3A12.11c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC3A12.11c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC3A12.11c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC3A12.11c Cell Cycle Data
GEO SPAC3A12.11c GEO profiles
PInt SPAC3A12.11c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC3A12.11c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC3A12.11c Fission yeast phenotypic data & analysis
SPD / RIKEN20/20C12Orfeome Localization Data
UniProtKB/SwissProtP87126Pre-mRNA-splicing factor cwf2
ModBaseP87126Database of comparative protein structure models
STRINGP87126Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593337RNA-binding protein Cwf2
RefSeq mRNANM_001018769972h- RNA-binding protein Cwf2 (cwf2), mRNA
European Nucleotide ArchiveAAF63625.1ENA Protein Mapping
European Nucleotide ArchiveCAC41387.1ENA Protein Mapping
UniParcUPI0000128732UniProt Archive

Literature for cwf2

Search: Europe PMC or PubMed

Release Version: PomBase:25_49 - 02 Feb 2015