cam1 (SPAC3A12.14)


Gene Standard Namecam1 Characterisation Statuspublished
Systematic IDSPAC3A12.14 Feature Typeprotein coding
Synonyms Name Description
Productcalmodulin Cam1 Product Size150aa, 16.91 kDa
Genomic Location Chromosome I, 1444658-1446333 (1676nt); CDS:1444930-1445508 (579nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
calcium ion binding39
Annotation ExtensionEvidenceWith/FromReference
protein binding887
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
ascospore formation57
Annotation ExtensionEvidenceWith/FromReference
ascospore-type prospore-specific spindle pole body remodeling9
Annotation ExtensionEvidenceWith/FromReference
establishment of protein localization to meiotic spindle pole body1
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle assembly18
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
barrier septum25
Annotation ExtensionEvidenceWith/FromReference
cell division site317
Annotation ExtensionEvidenceWith/FromReference
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
mating projection tip27
Annotation ExtensionEvidenceWith/FromReference
meiotic spindle pole body21
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle pole body220
Annotation ExtensionEvidenceWith/FromReference
new growing cell tip5
Annotation ExtensionEvidenceWith/FromReference
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
old growing cell tip9
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
decreased cell population growth at high temperatureE4 (E31V, E67V, E104V)178
E1 (E67V, E104V, E140V)
E34 (E31V, E67V)
E14 (E67V, E104V)
E13 (E67V, E140V)
decreased sporulation frequencycam1-D116 (R116D)103
cam1-F116 (R116F)
cam1-S116 (R116S)
inviable vegetative cell populationE0 (E31V, E67V, E104V, E140V)1455
E3 (E31V, E67V, E140V)
cam1Δ
E4 (E31V, E67V, E104V)
normal growth on clotrimazolecam1+ (wild type)13
normal growth on micafungincam1+ (wild type)5
normal growth on terbinafinecam1+ (wild type)41
normal vegetative cell population growth ratecam1-F116 (R116F)80
sensitive to 4-amino-1-methyl-1H-imidazole-5-carboselenoamidecam1+/cam1- (heterozygous diploid)8
sensitive to tamoxifencam1+ (wild type)58
sensitive to tingenonecam1+/cam1- (heterozygous diploid)7
viable vegetative cell populationE134 (E67V)3850
E24 (E31V, E104V)
E124 (E104V)
E12 (E104, E140V)
cam1-22,117 (D22Y, R117F)
E23 (E31V, E140V)
E234 (E31V)
E13 (E67V, E140V)
E123 (E140V)
E2 (E31V, E104V, E140V)
E1 (E67V, E104V, E140V)
E4 (E31V, E67V, E104V)
E14 (E67V, E104V)

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic chromosome congressionE14 (E67V, E104V)6
abnormal mitotic spindleE14 (E67V, E104V)62
abnormal spindle pole body morphology during meiosis IIcam1-22,117 (D22Y, R117F)4
abnormal sporulationcam1-EF3 (E106V)178
cam1-EF2 (E106V)
abolished prospore membrane formationcam1-22,117 (D22Y, R117F)9
abolished protein localization to meiotic spindle pole body5
affects localization of spo15cam1-22,117 (D22Y, R117F)
affects localization of spo2cam1-22,117 (D22Y, R117F)
affects localization of spo13cam1-22,117 (D22Y, R117F)
abolished sporulationcam1-22,117 (D22Y, R117F)56
cam1-22 (D22Y)
decreased protein level during meiotic cell cycle5
affecting spo15cam1-22,117 (D22Y, R117F)
decreased protein level during vegetative growth153
affecting cam1cam1-F116 (R116F)
increased protein degradation during nitrogen starvation1
affecting cam1cam1-F116 (R116F)
inviable after spore germinationcam1Δ815
inviable cell at high temperatureE13 (E67V, E140V)20
E4 (E31V, E67V, E104V)
E1 (E67V, E104V, E140V)
E34 (E31V, E67V)
inviable sporecam1Δ476
inviable vegetative cellE4 (E31V, E67V, E104V)1358
E0 (E31V, E67V, E104V, E140V)
E3 (E31V, E67V, E140V)
mitotic chromosome fragmentation upon segregationE14 (E67V, E104V)5
mitotic metaphase/anaphase transition delayE14 (E67V, E104V)20
normal conjugationcam1-F116 (R116F)50
normal cytosolic calcium levelcam1-12
normal interphase cytoplasmic microtubulesE14 (E67V, E104V)8
normal meiosiscam1-F116 (R116F)150
normal protein level during meiotic cell cycle2
affecting cam1cam1-EF3 (E106V)
affecting cam1cam1-22 (D22Y)
affecting cam1cam1-EF4 (E142V)
affecting cam1cam1-EF1 (E33V)
affecting cam1cam1-EF2 (E106V)
normal protein localization to meiotic spindle pole body9
affecting pcp1cam1-22,117 (D22Y, R117F)
affecting sad1cam1-22,117 (D22Y, R117F)
affecting sid4cam1-22,117 (D22Y, R117F)
affecting alp4cam1-22,117 (D22Y, R117F)
affecting cut12cam1-22,117 (D22Y, R117F)
normal RNA level during vegetative growth133
affecting cam1cam1-F116 (R116F)
normal sporulationcam1-EF1 (E33V)53
cam1-EF4 (E142V)
unequal mitotic sister chromatid segregationE14 (E67V, E104V)64
viable cell at high temperatureE24 (E31V, E104V)6
E134 (E67V)
E2 (E31V, E104V, E140V)
E12 (E104, E140V)
E23 (E31V, E140V)
E124 (E104V)
E14 (E67V, E104V)
E234 (E31V)
E123 (E140V)
viable cell at low temperatureE14 (E67V, E104V)2
E23 (E31V, E140V)
E234 (E31V)
E12 (E104, E140V)
E34 (E31V, E67V)
E4 (E31V, E67V, E104V)
E134 (E67V)
E123 (E140V)
E1 (E67V, E104V, E140V)
E24 (E31V, E104V)
E124 (E104V)
E13 (E67V, E140V)
E2 (E31V, E104V, E140V)
viable vegetative cellE124 (E104V)3659
E134 (E67V)
E34 (E31V, E67V)
E2 (E31V, E104V, E140V)
E23 (E31V, E140V)
E14 (E67V, E104V)
E13 (E67V, E140V)
E123 (E140V)
E24 (E31V, E104V)
E234 (E31V)
E1 (E67V, E104V, E140V)
E12 (E104, E140V)
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in cam1 calmodulin Cam1
FYPO affected by mutation in cmk1 calcium/calmodulin-dependent protein kinase Cmk1
FYPO affected by mutation in prp2 U2AF large subunit (U2AF-59)
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1444658..1444932, 1445059..1446333
Intron1444933..1445058
mRNA1444658..1446333
5' UTR1444658..1444929PMID:21511999
CDS1444930..1444932, 1445059..1445508
3' UTR1445509..1446333PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13499 Pfam IPR002048 EF-hand domain 85 145 6
PF13499 Pfam IPR002048 EF-hand domain 14 74 6
SM00054 SMART IPR002048 EF-hand domain 86 114 9
SM00054 SMART IPR002048 EF-hand domain 49 77 9
SM00054 SMART IPR002048 EF-hand domain 13 41 9
SM00054 SMART IPR002048 EF-hand domain 122 150 9
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 58 70 12
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 22 34 12
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 131 143 12
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 95 107 12
PS50222 Prosite Profiles IPR002048 EF-hand domain 45 80 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 118 150 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 82 117 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 9 44 20
PTHR23050 HMMPANTHER 4 150 3
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 71 148 20
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 5 70 20
SSF47473 SuperFamily IPR011992 EF-hand domain pair 3 147 21

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000276EF handTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000276
PBO:0000979calmodulin familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000979

Protein Properties

Ave. residue weight 112.70 Da
Charge -20.50
Codon Adaptation Index 0.54
Isoelectric point 3.90
Molecular weight 16.91 kDa
Number of residues 150
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-threonineT7, T132 1085
Annotation ExtensionEvidenceResidueReference
IDA T132 PMID:25720772
IDA T7 PMID:25720772
phosphorylated residue 2511
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelpresent during GO:0072690Northern assay evidencePMID:3035538

Quantitative Gene Expression

View graphical display of gene expression data for cam1 (SPAC3A12.14)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
167122during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
157242during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
165055during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
153299during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
160857during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
25597.96during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
39964.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8.9during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by vertebrate vCaMfull PMID:7657644
functionally complements S. cerevisiae CMD1full PMID:7657644
is not functionally complemented by S. cerevisiae CMD1full PMID:7657644
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC3A12.14 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bysog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
affinity captured bymyo1myosin type I Affinity Capture-WesternPMID:11260263
affinity captured bynak1PAK-related kinase Nak1 Affinity Capture-MSPMID:23462181
affinity captured byrng2IQGAP Affinity Capture-WesternPMID:9635188
forms complex withmob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Reconstituted ComplexPMID:15004232
forms complex withpcp1pericentrin Pcp1 Reconstituted ComplexPMID:15004232
binds DNA-binding domain construct withmyo52myosin type V Two-hybridPMID:18003699
affinity capturesrng2IQGAP Affinity Capture-WesternPMID:9635188
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC3A12.14 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
rescued byspo15mitotic and mieotic spindle pole body protein Spo15 Phenotypic SuppressionPMID:20833892
External References
Database Identifier Description
NBRP SPAC3A12.14 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC3A12.14 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC3A12.14 BioGRID Interaction Datasets
Expression Viewer SPAC3A12.14 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC3A12.14 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC3A12.14 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC3A12.14 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC3A12.14 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC3A12.14 Transcriptome Viewer (Bähler Lab)
GEO SPAC3A12.14 GEO profiles
PInt SPAC3A12.14 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC3A12.14 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC3A12.14 Fission yeast phenotypic data & analysis
Cyclebase SPAC3A12.14.1 Cell Cycle Data
SPD / RIKEN06/06C03Orfeome Localization Data
UniProtKB/SwissProtP05933Calmodulin
ModBaseP05933Database of comparative protein structure models
STRINGP05933Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593340calmodulin Cam1
RefSeq mRNANM_001018772972h- calmodulin Cam1 (cam1), mRNA
European Nucleotide ArchiveAAA35291.1ENA Protein Mapping
European Nucleotide ArchiveCAB08742.1ENA Protein Mapping
UniParcUPI0000126E6AUniProt Archive

Literature for cam1

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016