cam1 (SPAC3A12.14)


Gene Standard Namecam1 Characterisation Statuspublished
Systematic IDSPAC3A12.14 Feature Typeprotein coding
Synonyms Name Description
Productcalmodulin Cam1 Product Size150aa, 16.91 kDa
Genomic Location Chromosome I, 1444658-1446333 (1676nt); CDS:1444930-1445508 (579nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
calcium ion binding44
Annotation ExtensionEvidenceWith/FromReference
protein binding848
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
ascospore formation76
Annotation ExtensionEvidenceWith/FromReference
ascospore-type prospore-specific spindle pole body remodeling7
Annotation ExtensionEvidenceWith/FromReference
maintenance of protein location in spindle pole body1
Annotation ExtensionEvidenceWith/FromReference
spindle assembly involved in mitosis18
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
barrier septum15
Annotation ExtensionEvidenceWith/FromReference
cell division site299
Annotation ExtensionEvidenceWith/FromReference
cytosol2316
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle pole body216
Annotation ExtensionEvidenceWith/FromReference
new growing cell tip4
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
old growing cell tip6
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growth at high temperatureE1 (E67V, E104V, E140V)Overexpression151
E13 (E67V, E140V)Overexpression
E14 (E67V, E104V)Endogenous
E34 (E31V, E67V)Overexpression
E4 (E31V, E67V, E104V)Overexpression
inviable vegetative cell populationcam1ΔNull1427
E0 (E31V, E67V, E104V, E140V)Overexpression
E3 (E31V, E67V, E140V)Overexpression
E4 (E31V, E67V, E104V)Overexpression
normal vegetative cell population growth ratecam1-F116 (R116F)Not specified57
viable vegetative cell populationE1 (E67V, E104V, E140V)Overexpression3781
E12 (E104, E140V)Overexpression
E123 (E140V)Overexpression
E124 (E104V)Overexpression
E13 (E67V, E140V)Overexpression
E134 (E67V)Overexpression
E14 (E67V, E104V)Overexpression
E2 (E31V, E104V, E140V)Overexpression
E23 (E31V, E140V)Overexpression
E234 (E31V)Overexpression
E24 (E31V, E104V)Overexpression
E4 (E31V, E67V, E104V)Overexpression

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic chromosome congressionE14 (E67V, E104V)Endogenous1
abnormal mitotic spindleE14 (E67V, E104V)Endogenous40
abolished mitotic sister chromatid separationE14 (E67V, E104V)Endogenous21
decreased protein level during vegetative growth80
affecting cam1cam1-F116 (R116F)Not specified
decreased sporulationcam1-D116 (R116D)Not specified65
cam1-F116 (R116F)Overexpression
cam1-F116 (R116F)Not specified
cam1-S116 (R116S)Not specified
increased protein degradation during nitrogen starvation1
affecting cam1cam1-F116 (R116F)Not specified
inviable after spore germinationcam1ΔNull793
inviable cell at high temperatureE1 (E67V, E104V, E140V)Overexpression22
E13 (E67V, E140V)Overexpression
E34 (E31V, E67V)Overexpression
E4 (E31V, E67V, E104V)Overexpression
inviable spore467
penetrance FYPO_EXT:0000001cam1ΔNull
inviable vegetative cellE0 (E31V, E67V, E104V, E140V)Overexpression1268
E3 (E31V, E67V, E140V)Overexpression
E4 (E31V, E67V, E104V)Overexpression
mitotic chromosome fragmentation upon segregationE14 (E67V, E104V)Endogenous5
mitotic metaphase/anaphase transition delayE14 (E67V, E104V)Endogenous9
normal conjugationcam1-F116 (R116F)Not specified40
normal immediate intracellular calcium spike following extracellular calcium stimuluscam1-1Not specified3
normal interphase cytoplasmic microtubulesE14 (E67V, E104V)Endogenous7
normal meiosiscam1-F116 (R116F)Not specified139
normal RNA level during vegetative growth91
affecting cam1cam1-F116 (R116F)Not specified
viable cell at high temperatureE12 (E104, E140V)Overexpression8
E123 (E140V)Overexpression
E124 (E104V)Overexpression
E134 (E67V)Overexpression
E14 (E67V, E104V)Overexpression
E2 (E31V, E104V, E140V)Overexpression
E23 (E31V, E140V)Overexpression
E234 (E31V)Overexpression
E24 (E31V, E104V)Overexpression
viable cell at low temperatureE1 (E67V, E104V, E140V)Overexpression2
E12 (E104, E140V)Overexpression
E123 (E140V)Overexpression
E124 (E104V)Overexpression
E13 (E67V, E140V)Overexpression
E134 (E67V)Overexpression
E14 (E67V, E104V)Overexpression
E2 (E31V, E104V, E140V)Overexpression
E23 (E31V, E140V)Overexpression
E234 (E31V)Overexpression
E24 (E31V, E104V)Overexpression
E34 (E31V, E67V)Overexpression
E4 (E31V, E67V, E104V)Overexpression
viable vegetative cellE1 (E67V, E104V, E140V)Overexpression3613
E12 (E104, E140V)Overexpression
E123 (E140V)Overexpression
E124 (E104V)Overexpression
E13 (E67V, E140V)Overexpression
E134 (E67V)Overexpression
E14 (E67V, E104V)Overexpression
E2 (E31V, E104V, E140V)Overexpression
E23 (E31V, E140V)Overexpression
E234 (E31V)Overexpression
E24 (E31V, E104V)Overexpression
E34 (E31V, E67V)Overexpression
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in cam1 calmodulin Cam1 PMID:2690071
FYPO affected by mutation in cmk1 calcium/calmodulin-dependent protein kinase Cmk1 PMID:10617667
FYPO affected by mutation in prp2 U2AF large subunit (U2AF-59) PMID:9917066
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
114446581444932
214450591446333

UTRs

Region Coordinates Reference
five_prime_UTR1444658..1444929PMID:21511999
three_prime_UTR1445509..1446333PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13499 Pfam IPR011992 EF-hand domain pair 15 74 7
PF13499 Pfam IPR011992 EF-hand domain pair 87 145 7
SM00054 SMART IPR002048 EF-hand domain 86 114 9
SM00054 SMART IPR002048 EF-hand domain 13 41 9
SM00054 SMART IPR002048 EF-hand domain 122 150 9
SM00054 SMART IPR002048 EF-hand domain 49 77 9
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 131 143 12
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 22 34 12
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 95 107 12
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 58 70 12
PS50222 Prosite Profiles IPR002048 EF-hand domain 45 80 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 82 117 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 9 44 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 118 150 20
PTHR23050 HMMPANTHER 4 150 3
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 71 148 20
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 5 70 20
SSF47473 SuperFamily 3 147 21

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000276EF handTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000276
PBO:0000979calmodulin familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000979

Protein Properties

Ave. residue weight 112.70 Da
Charge -20.50
Isoelectric point 3.90
Molecular weight 16.91 kDa
Number of residues 150
Modifications

Protein Modifications

Term NameResidueCount
phosphorylated residue 1922
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelpresent during GO:0072690Northern assay evidencePMID:3035538

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
167122during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
157242during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
165055during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
153299during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
25597.96during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
160857during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
39964.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8.9during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by vertebrate vCaMfull PMID:7657644
functionally complements S. cerevisiae CMD1full PMID:7657644
is not functionally complemented by S. cerevisiae CMD1full PMID:7657644
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
pcp1pericentrin Pcp1 Reconstituted ComplexPMID:11864908
Reconstituted ComplexPMID:15004232
rng2IQGAP Affinity Capture-WesternPMID:9635188
mob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Reconstituted ComplexPMID:15004232
myo1myosin type I Affinity Capture-WesternPMID:11260263
sog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
nak1PAK-related kinase Nak1 Affinity Capture-MSPMID:23462181
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
myo52myosin type V Two-hybridPMID:18003699
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
spo15sporulation protein Spo15 Phenotypic SuppressionPMID:20833892
External References
Database Identifier Description
NBRP SPAC3A12.14 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC3A12.14 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC3A12.14 BioGRID Interaction Datasets
Expression Viewer SPAC3A12.14 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC3A12.14 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC3A12.14 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC3A12.14 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC3A12.14 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC3A12.14 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC3A12.14 Cell Cycle Data
GEO SPAC3A12.14 GEO profiles
PInt SPAC3A12.14 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC3A12.14 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC3A12.14 Fission yeast phenotypic data & analysis
SPD / RIKEN06/06C03Orfeome Localization Data
UniProtKB/SwissProtP05933Calmodulin
ModBaseP05933Database of comparative protein structure models
STRINGP05933Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593340calmodulin Cam1
RefSeq mRNANM_001018772972h- calmodulin Cam1 (cam1), mRNA
European Nucleotide ArchiveAAA35291.1ENA Protein Mapping
European Nucleotide ArchiveCAB08742.1ENA Protein Mapping
UniParcUPI0000126E6AUniProt Archive

Literature for cam1

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015