cam1 (SPAC3A12.14)


Gene Standard Namecam1 Characterisation Statuspublished
Systematic IDSPAC3A12.14 Feature Typeprotein coding
Synonyms Name Description
Productcalmodulin Cam1 Product Size150aa, 16.91 kDa
Genomic Location Chromosome I, 1444658-1446333 (1676nt); CDS:1444930-1445508 (579nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005509calcium ion bindingIDAPMID:765764448
GO:0005515protein bindingIPIrng2PMID:9635188831
IPImyo1PMID:11260263
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030437ascospore formationIMPPMID:269007175
GO:0031322ascospore-type prospore-specific spindle pole body remodelingIMPPMID:208338927
GO:0071990maintenance of protein location in spindle pole body1
localizes spo15IMPPMID:20833892
GO:0090307spindle assembly involved in mitosisIMPPMID:926446717
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000935barrier septumIDAPMID:926446715
GO:0032153cell division siteIDAPMID:9264467301
GO:0005829cytosolIDAPMID:168233722315
GO:0044732mitotic spindle pole bodyIDAPMID:9264467216
during ascospore formationIDAPMID:20833892
GO:0035841new growing cell tipIDAPMID:92644674
GO:0005634nucleusIDAPMID:168233722740
GO:0035840old growing cell tipIDAPMID:92644676
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000082decreased cell population growth at high temperatureCell growth assayE14 (E67V, E104V)EndogenousPMID:9264467148
Cell growth assayE1 (E67V, E104V, E140V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE34 (E31V, E67V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE13 (E67V, E140V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE4 (E31V, E67V, E104V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
FYPO:0002061inviable vegetative cell populationCell growth assayE3 (E31V, E67V, E140V)OverexpressionPECO:0000137, PECO:0000102, PECO:0000006PMID:76576441338
Microscopycam1ΔNullPMID:20473289
Cell growth assayE0 (E31V, E67V, E104V, E140V)OverexpressionPECO:0000137, PECO:0000102, PECO:0000004PMID:7657644
Cell growth assayE3 (E31V, E67V, E140V)OverexpressionPECO:0000137, PECO:0000102, PECO:0000004PMID:7657644
Cell growth assayE4 (E31V, E67V, E104V)OverexpressionPECO:0000137, PECO:0000102, PECO:0000005PMID:7657644
Cell growth assayE3 (E31V, E67V, E140V)OverexpressionPECO:0000137, PECO:0000102, PECO:0000005PMID:7657644
Cell growth assayE0 (E31V, E67V, E104V, E140V)OverexpressionPECO:0000137, PECO:0000102, PECO:0000005PMID:7657644
Cell growth assayE0 (E31V, E67V, E104V, E140V)OverexpressionPECO:0000137, PECO:0000102, PECO:0000006PMID:7657644
Microscopycam1ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0001420normal vegetative cell population growth rateCell growth assaycam1-F116 (R116F)Not specifiedPMID:269007151
FYPO:0002060viable vegetative cell populationCell growth assayE14 (E67V, E104V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000006PMID:76576443759
Cell growth assayE14 (E67V, E104V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000005PMID:7657644
Cell growth assayE12 (E104, E140V)OverexpressionPECO:0000137, PECO:0000102, PECO:0000006PMID:7657644
Cell growth assayE14 (E67V, E104V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000006PMID:7657644
Cell growth assayE12 (E104, E140V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000005PMID:7657644
Cell growth assayE124 (E104V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000005PMID:7657644
Cell growth assayE134 (E67V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000005PMID:7657644
Cell growth assayE14 (E67V, E104V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000005PMID:7657644
Cell growth assayE24 (E31V, E104V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000006PMID:7657644
Cell growth assayE1 (E67V, E104V, E140V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000005PMID:7657644
Cell growth assayE123 (E140V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000006PMID:7657644
Cell growth assayE4 (E31V, E67V, E104V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000006PMID:7657644
Cell growth assayE234 (E31V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000006PMID:7657644
Cell growth assayE234 (E31V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000005PMID:7657644
Cell growth assayE124 (E104V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000004PMID:7657644
Cell growth assayE13 (E67V, E140V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000005PMID:7657644
Cell growth assayE2 (E31V, E104V, E140V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000004PMID:7657644
Cell growth assayE1 (E67V, E104V, E140V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000005PMID:7657644
Cell growth assayE23 (E31V, E140V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000006PMID:7657644
Cell growth assayE13 (E67V, E140V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000006PMID:7657644
Cell growth assayE14 (E67V, E104V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000004PMID:7657644
Cell growth assayE23 (E31V, E140V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000004PMID:7657644
Cell growth assayE123 (E140V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000004PMID:7657644
Cell growth assayE2 (E31V, E104V, E140V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000005PMID:7657644
Cell growth assayE234 (E31V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000004PMID:7657644
Cell growth assayE24 (E31V, E104V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000005PMID:7657644
Cell growth assayE23 (E31V, E140V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000005PMID:7657644
Cell growth assayE12 (E104, E140V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000004PMID:7657644
Cell growth assayE124 (E104V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000006PMID:7657644
Cell growth assayE134 (E67V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000004PMID:7657644
Cell growth assayE123 (E140V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000005PMID:7657644
Cell growth assayE24 (E31V, E104V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000004PMID:7657644
Cell growth assayE2 (E31V, E104V, E140V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000006PMID:7657644
Cell growth assayE134 (E67V)OverexpressionPECO:0000102, PECO:0000137, PECO:0000006PMID:7657644

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000030abnormal mitotic chromosome congressionMicroscopyE14 (E67V, E104V)EndogenousPECO:0000004PMID:92644671
FYPO:0000338abnormal mitotic spindleMicroscopyE14 (E67V, E104V)EndogenousPECO:0000004PMID:926446735
FYPO:0001946abolished mitotic chromosome separationMicroscopyE14 (E67V, E104V)EndogenousPECO:0000004PMID:926446712
FYPO:0001324decreased protein level during vegetative growth60
affecting cam1Western blot assaycam1-F116 (R116F)Not specifiedPMID:2690071
FYPO:0000584decreased sporulationMicroscopycam1-S116 (R116S)Not specifiedPMID:269007161
Microscopycam1-F116 (R116F)OverexpressionPMID:2690071
Microscopycam1-F116 (R116F)Not specifiedPMID:2690071
Microscopycam1-D116 (R116D)Not specifiedPMID:2690071
FYPO:0002277increased protein degradation during nitrogen starvation1
affecting cam1Western blot assaycam1-F116 (R116F)Not specifiedPMID:2690071
FYPO:0000316inviable after spore germinationMicroscopycam1ΔNullPECO:0000137PMID:3035538777
FYPO:0001801inviable cell at high temperatureCell growth assayE4 (E31V, E67V, E104V)OverexpressionPECO:0000137, PECO:0000102PMID:765764423
Cell growth assayE13 (E67V, E140V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE34 (E31V, E67V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE1 (E67V, E104V, E140V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
FYPO:0002151inviable spore465
penetrance FYPO_EXT:0000001Microscopycam1ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0001489inviable vegetative cellCell growth assayE4 (E31V, E67V, E104V)OverexpressionPECO:0000137, PECO:0000102, PECO:0000005PMID:76576441254
Cell growth assayE0 (E31V, E67V, E104V, E140V)OverexpressionPECO:0000137, PECO:0000102, PECO:0000006PMID:7657644
Cell growth assayE3 (E31V, E67V, E140V)OverexpressionPECO:0000137, PECO:0000102, PECO:0000006PMID:7657644
Cell growth assayE0 (E31V, E67V, E104V, E140V)OverexpressionPECO:0000137, PECO:0000102, PECO:0000004PMID:7657644
Cell growth assayE3 (E31V, E67V, E140V)OverexpressionPECO:0000137, PECO:0000102, PECO:0000004PMID:7657644
Cell growth assayE3 (E31V, E67V, E140V)OverexpressionPECO:0000137, PECO:0000102, PECO:0000005PMID:7657644
Cell growth assayE0 (E31V, E67V, E104V, E140V)OverexpressionPECO:0000137, PECO:0000102, PECO:0000005PMID:7657644
FYPO:0000283mitotic chromosome fragmentation upon segregationMicroscopyE14 (E67V, E104V)EndogenousPECO:0000004PMID:92644674
FYPO:0000324mitotic metaphase/anaphase transition delayMicroscopyE14 (E67V, E104V)EndogenousPECO:0000004PMID:92644678
FYPO:0001317normal RNA level during vegetative growth80
affecting cam1Transcript expression level evidencecam1-F116 (R116F)Not specifiedPMID:2690071
FYPO:0000760normal conjugationMicroscopycam1-F116 (R116F)Not specifiedPMID:269007136
FYPO:0001461normal immediate intracellular calcium spike following extracellular calcium stimulusSubstance quantificationcam1-1Not specifiedPMID:218116073
FYPO:0001400normal interphase cytoplasmic microtubulesMicroscopyE14 (E67V, E104V)EndogenousPECO:0000004PMID:92644677
FYPO:0000478normal meiosisMicroscopycam1-F116 (R116F)Not specifiedPMID:2690071139
FYPO:0000282viable cell at high temperatureCell growth assayE234 (E31V)OverexpressionPECO:0000137, PECO:0000102PMID:76576448
Cell growth assayE14 (E67V, E104V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE2 (E31V, E104V, E140V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE123 (E140V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE12 (E104, E140V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE134 (E67V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE24 (E31V, E104V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE124 (E104V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE23 (E31V, E140V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
FYPO:0002171viable cell at low temperatureCell growth assayE124 (E104V)OverexpressionPECO:0000137, PECO:0000102PMID:76576442
Cell growth assayE234 (E31V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE13 (E67V, E140V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE34 (E31V, E67V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE24 (E31V, E104V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE23 (E31V, E140V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE12 (E104, E140V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE14 (E67V, E104V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE123 (E140V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE2 (E31V, E104V, E140V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE1 (E67V, E104V, E140V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE134 (E67V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE4 (E31V, E67V, E104V)OverexpressionPECO:0000137, PECO:0000102PMID:7657644
FYPO:0001491viable vegetative cellCell growth assayE14 (E67V, E104V)OverexpressionPECO:0000005, PECO:0000137, PECO:0000102PMID:76576443607
Cell growth assayE2 (E31V, E104V, E140V)OverexpressionPECO:0000005, PECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE124 (E104V)OverexpressionPECO:0000005, PECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE1 (E67V, E104V, E140V)OverexpressionPECO:0000005, PECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE23 (E31V, E140V)OverexpressionPECO:0000005, PECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE12 (E104, E140V)OverexpressionPECO:0000005, PECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE234 (E31V)OverexpressionPECO:0000005, PECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE13 (E67V, E140V)OverexpressionPECO:0000005, PECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE134 (E67V)OverexpressionPECO:0000005, PECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE34 (E31V, E67V)OverexpressionPECO:0000005, PECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE24 (E31V, E104V)OverexpressionPECO:0000005, PECO:0000137, PECO:0000102PMID:7657644
Cell growth assayE123 (E140V)OverexpressionPECO:0000005, PECO:0000137, PECO:0000102PMID:7657644
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in cam1 calmodulin Cam1 PMID:2690071
FYPO affected by mutation in cmk1 calcium/calmodulin-dependent protein kinase Cmk1 PMID:10617667
FYPO affected by mutation in prp2 U2AF large subunit (U2AF-59) PMID:9917066
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
114446581444932
214450591446333

UTRs

Region Coordinates Reference
five_prime_UTR1444658..1444929PMID:21511999
three_prime_UTR1445509..1446333PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13499 Pfam IPR011992 EF-hand domain pair 87 145 7
PF13499 Pfam IPR011992 EF-hand domain pair 15 74 7
SM00054 SMART IPR002048 EF-hand domain 86 114 9
SM00054 SMART IPR002048 EF-hand domain 13 41 9
SM00054 SMART IPR002048 EF-hand domain 122 150 9
SM00054 SMART IPR002048 EF-hand domain 49 77 9
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 95 107 12
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 58 70 12
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 22 34 12
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 131 143 12
PS50222 Prosite Profiles IPR002048 EF-hand domain 82 117 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 45 80 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 9 44 20
PS50222 Prosite Profiles IPR002048 EF-hand domain 118 150 20
PTHR23050 HMMPANTHER 4 150 1
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 71 148 20
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 5 70 20
SSF47473 SuperFamily 3 147 21

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000276EF handTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000276
PBO:0000979calmodulin familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000979

Protein Properties

Ave. residue weight 112.70 Da
Charge -20.50
Isoelectric point 3.90
Molecular weight 16.91 kDa
Number of residues 150
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residueNASPMID:195477441922
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelpresent during GO:0072690Northern assay evidencePMID:3035538

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
167122during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
157242during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
165055during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
153299during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
25597.96during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
160857during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
39964.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8.9during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by vertebrate vCaMfull PMID:7657644
functionally complements S. cerevisiae CMD1full PMID:7657644
is not functionally complemented by S. cerevisiae CMD1full PMID:7657644
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
spo15sporulation protein Spo15 Phenotypic SuppressionPMID:20833892
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
pcp1pericentrin Pcp1 Reconstituted ComplexPMID:11864908
Reconstituted ComplexPMID:15004232
rng2IQGAP Affinity Capture-WesternPMID:9635188
mob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Reconstituted ComplexPMID:15004232
myo1myosin type I Affinity Capture-WesternPMID:11260263
sog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
nak1PAK-related kinase Nak1 Affinity Capture-MSPMID:23462181
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
myo52myosin type V Two-hybridPMID:18003699
External References
Database Identifier Description
NBRP SPAC3A12.14 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC3A12.14 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC3A12.14 BioGRID Interaction Datasets
Expression Viewer SPAC3A12.14 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC3A12.14 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC3A12.14 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC3A12.14 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC3A12.14 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC3A12.14 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC3A12.14 Cell Cycle Data
GEO SPAC3A12.14 GEO profiles
PInt SPAC3A12.14 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC3A12.14 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC3A12.14 Fission yeast phenotypic data & analysis
SPD / RIKEN06/06C03Orfeome Localization Data
UniProtKB/SwissProtP05933Calmodulin
ModBaseP05933Database of comparative protein structure models
STRINGP05933Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593340calmodulin Cam1
RefSeq mRNANM_001018772972h- calmodulin Cam1 (cam1), mRNA
European Nucleotide ArchiveAAA35291.1ENA Protein Mapping
European Nucleotide ArchiveCAB08742.1ENA Protein Mapping
UniParcUPI0000126E6AUniProt Archive

Literature for cam1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014