cdc3 (SPAC4A8.15c)

Gene Standard Namecdc3 Characterisation Statuspublished
Systematic IDSPAC4A8.15c Feature Typeprotein coding
Synonyms Name Description
Productprofilin Product Size127aa, 13.41 kDa
Genomic Location Chromosome I, 2579514-2578956 (559nt); CDS:2579478-2578990 (489nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
actin monomer binding2
Annotation ExtensionEvidenceWith/FromReference
guanyl-nucleotide exchange factor activity36
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
actin cortical patch organization24
Annotation ExtensionEvidenceWith/FromReference
actin filament polymerization21
Annotation ExtensionEvidenceWith/FromReference
actomyosin contractile ring actin filament bundle assembly4
Annotation ExtensionEvidenceWith/FromReference
mitotic actomyosin contractile ring assembly28
Annotation ExtensionEvidenceWith/FromReference
plasma membrane fusion involved in cytogamy6
Annotation ExtensionEvidenceWith/FromReference
positive regulation of GTPase activity53
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actin cortical patch47
Annotation ExtensionEvidenceWith/FromReference
cell cortex of cell tip42
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
mating projection10
Annotation ExtensionEvidenceWith/FromReference
medial cortex32
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growth at high temperature151
expressivity FYPO_EXT:0000001cdc3-124 (E43K)Not specified
inviable vegetative cell populationcdc3-124 (E43K)Not specified1427
normal cell population growth at low temperaturecdc3-124 (E43K)Not specified10

Cell Phenotype

Term NameAlleleExpressionCount
abnormal actin cortical patch morphologycdc3-313Not specified2
abnormal actin filament organizationcdc3+ (wild type)Overexpression10
abolished actomyosin contractile ring assemblycdc3+ (wild type)Overexpression64
cdc3-124 (E43K)Endogenous
abolished protein localization to medial cortex during vegetative growth10
affecting cdc12cdc3-313Not specified
dispersed actin cortical patch localization during mitotic interphasecdc3-313Not specified3
elongated multinucleate vegetative cellcdc3-124 (E43K)34
filamentous actin absentcdc3ΔNull2
increased spontaneous diploidizationcdc3-313Not specified24
inviable after spore germination, without cell division, swollen dumbbell-shaped cell2
expressivity FYPO_EXT:0000003cdc3ΔNull
inviable elongated dumbbell-shaped cellcdc3+ (wild type)Overexpression6
cdc3-124 (E43K)
inviable elongated multinucleate vegetative cell with abnormal septum morphologycdc3-313Not specified2
inviable, elongated, multinucleate dumbbell-shaped cellcdc3-124 (E43K)Endogenous4
cdc3-6Not specified
mislocalized septumcdc3-124 (E43K)31
normal cytokinesis checkpointcdc3Not specified3
normal mitosiscdc3-124 (E43K)Endogenous10
normal protein localization to cell tip17
affecting ags1cdc3-6Not specified
Ensembl transcript structure with UTRs, exons and introns


Exon Start End
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00235 Pfam IPR005455 Profilin 2 122 1
SM00392 SMART IPR005455 Profilin 1 127 1
PS00414 Prosite Patterns IPR027310 Profilin conserved site 1 8 1
PTHR11604 HMMPANTHER IPR005455 Profilin 1 127 1
PTHR11604:SF0 HMMPANTHER 1 127 1
3.30.450.30 Gene3D 1 127 2
SSF55770 SuperFamily IPR005455 Profilin 2 127 1
PR01640 PRINTS IPR005455 Profilin 1 12 1
PR01640 PRINTS IPR005455 Profilin 100 113 1
PR01640 PRINTS IPR005455 Profilin 49 63 1
PR01640 PRINTS IPR005455 Profilin 68 77 1
PR01640 PRINTS IPR005455 Profilin 113 126 1
PR01640 PRINTS IPR005455 Profilin 25 38 1
PR00392 PRINTS IPR005455 Profilin 37 57 1
PR00392 PRINTS IPR005455 Profilin 109 126 1
PR00392 PRINTS IPR005455 Profilin 61 75 1
PR00392 PRINTS IPR005455 Profilin 18 27 1
PR00392 PRINTS IPR005455 Profilin 3 12 1
PR00392 PRINTS IPR005455 Profilin 96 109 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 105.60 Da
Charge -0.50
Isoelectric point 5.82
Molecular weight 13.41 kDa
Number of residues 127
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
239543during GO:0000084PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
247873during GO:0000087PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
48285.67during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
232138during GO:0072690PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
60595.21during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
15during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
cdc12formin Cdc12 Reconstituted ComplexPMID:18305104
Reconstituted ComplexPMID:9105045
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Synthetic Growth DefectPMID:23297348
acp1F-actin capping protein alpha subunit Synthetic LethalityPMID:16866873
acp2F-actin capping protein beta subunit Acp2 Synthetic LethalityPMID:16866873
Phenotypic Enhancement
Synthetic LethalityPMID:15743909
rng8contractile ring myosin V regulator Rng8 Synthetic Growth DefectPMID:24798735
arp3Arp2/3 protein complex, actin-like protein subunit Arp3 Phenotypic SuppressionPMID:8978671
arp2ARP2/3 actin-organizing complex subunit Arp2 Synthetic RescuePMID:10588653
ssp1Ca2+/calmodulin-dependent (CaMMK)-like protein kinase Ssp1 Phenotypic SuppressionPMID:10233158
cdc16two-component GAP Cdc16 Phenotypic EnhancementPMID:20805322
adf1actin depolymerizing factor, cofilin Synthetic LethalityPMID:16467379
Phenotypic Enhancement
sid2NDR kinase Sid2 Phenotypic SuppressionPMID:20805322
imp2contractile ring protein Imp2 Synthetic Growth DefectPMID:19139265
sec8exocyst complex subunit Sec8 Phenotypic EnhancementPMID:21148300
cdc12formin Cdc12 Synthetic LethalityPMID:9105045
arc1ARP2/3 actin-organizing complex subunit Sop2 Synthetic RescuePMID:8978670
Synthetic Growth Defect
ain1alpha-actinin Synthetic LethalityPMID:11294907
pom1DYRK family protein kinase Pom1 Synthetic LethalityPMID:9573052
asp1inositol hexakisphosphate kinase/inositol pyrophosphate synthase (predicted) Synthetic RescuePMID:10388810
blt1ubiquitin domain-like protein Blt1 Synthetic Growth DefectPMID:24790095
act1actin Act1 Synthetic Growth DefectPMID:10371213
Synthetic LethalityPMID:23589458
Synthetic Growth Defect
Phenotypic SuppressionPMID:8207058
wee1M phase inhibitor protein kinase Wee1 Phenotypic SuppressionPMID:20805322
External References
Database Identifier Description
NBRP SPAC4A8.15c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC4A8.15c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC4A8.15c BioGRID Interaction Datasets
Expression Viewer SPAC4A8.15c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC4A8.15c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC4A8.15c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC4A8.15c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC4A8.15c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC4A8.15c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC4A8.15c Cell Cycle Data
GEO SPAC4A8.15c GEO profiles
PInt SPAC4A8.15c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC4A8.15c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC4A8.15c Fission yeast phenotypic data & analysis
SPD / RIKEN41/41C06Orfeome Localization Data
ModBaseP39825Database of comparative protein structure models
STRINGP39825Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593827profilin
RefSeq mRNANM_001019256972h- profilin (cdc3), mRNA
ePDB3D9YThe European PDB
ePDB3DAVThe European PDB
European Nucleotide Archive-ENA Protein Mapping
European Nucleotide ArchiveCAB38578.1ENA Protein Mapping
UniParcUPI000013231DUniProt Archive

Literature for cdc3

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015