cdc3 (SPAC4A8.15c)


Gene Standard Namecdc3 Characterisation Statuspublished
Systematic IDSPAC4A8.15c Feature Typeprotein coding
Synonyms Name Description
Productprofilin Product Size127aa, 13.41 kDa
Genomic Location Chromosome I, 2579514-2578956 (559nt); CDS:2579478-2578990 (489nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
actin monomer binding2
Annotation ExtensionEvidenceWith/FromReference
guanyl-nucleotide exchange factor activity36
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
actin cortical patch organization24
Annotation ExtensionEvidenceWith/FromReference
actin filament polymerization19
Annotation ExtensionEvidenceWith/FromReference
mitotic actomyosin contractile ring assembly27
Annotation ExtensionEvidenceWith/FromReference
mitotic contractile ring actin filament bundle assembly3
Annotation ExtensionEvidenceWith/FromReference
plasma membrane fusion involved in cytogamy6
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actin cortical patch47
Annotation ExtensionEvidenceWith/FromReference
cell cortex of cell tip48
Annotation ExtensionEvidenceWith/FromReference
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
mating projection28
Annotation ExtensionEvidenceWith/FromReference
medial cortex33
Annotation ExtensionEvidenceWith/FromReference
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
decreased cell population growth at high temperaturecdc3-124 (E43K)178
inviable vegetative cell populationcdc3-2111455
cdc3-5
cdc3Δ
cdc3-181
cdc3-124 (E43K)
normal cell population growth at low temperaturecdc3-124 (E43K)29
viable vegetative cell populationcdc3-2113850
cdc3-5
cdc3-181
cdc3-124 (E43K)

Cell Phenotype

Term NameGenotypesCount
abnormal actin cortical patch morphologycdc3-3132
abnormal actin filament organization during vegetative growthcdc3+10
abnormal actomyosin contractile ring assemblycdc3-516
abnormal septum morphology during vegetative growthcdc3-124 (E43K)49
abolished actomyosin contractile ring assemblycdc3Δ5
cdc3-124 (E43K)
cdc3+
abolished cytokinesiscdc3-124 (E43K)94
abolished protein localization to medial cortex during vegetative growth22
affects localization of cdc12cdc3-313
affects localization of sid2cdc3-124 (E43K)
actomyosin contractile ring absentcdc3-124 (E43K)9
decreased protein kinase activity56
affecting activity of sid2cdc3-124 (E43K)
dispersed actin cortical patch localization during mitotic interphasecdc3-3133
elongated multinucleate vegetative cellcdc3-124 (E43K)46
filamentous actin absentcdc3Δ2
inviable after spore germination, without cell division, swollen binucleate cell with central constrictioncdc3Δ3
inviable elongated multinucleate aseptate vegetative cellcdc3-124 (E43K)11
inviable elongated multinucleate vegetative cell with abnormal septum morphologycdc3-3132
inviable elongated multinucleate vegetative cell with central constrictioncdc3-124 (E43K)4
inviable elongated vegetative cell with central constrictioncdc3-124 (E43K)5
cdc3Δ
cdc3+
mislocalized septum during vegetative growthcdc3-124 (E43K)45
normal cytokinesis checkpointcdc3-124 (E43K)5
normal mitosiscdc3-124 (E43K)5
normal protein level during vegetative growth91
affecting sid2cdc3-124 (E43K)
normal protein localization to mitotic spindle pole body20
affecting sid2cdc3-124 (E43K)

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased cell population growth on glucose carbon sourcecdc3-124 (E43K), 431-481 deleted (431-481)
cdc3-124 (E43K), clp1Δ
inviable vegetative cell populationsop2-1 (G207D), cdc3-124 (E43K)
cdc3-124 (E43K), sop2-1 (G207D), arc1+
viable vegetative cell populationsop2-1 (G207D), cdc3-124 (E43K)

Cell Phenotype

Term NameGenotypes
abolished protein phosphorylation during vegetative growth
affecting nak1cdc3-124 (E43K), cdc16-116, nak1-5A (S479A, S491A, S501A, S533A, S561A)
increased protein localization to cell tip during mitosis
affects localization of act1sop2-1 (G207D), cdc3-124 (E43K)
inviable elongated multinucleate vegetative cell with central constrictioncdc3-124 (E43K), wat1-1 (S278F)
normal protein localization to cell tip
affecting ags1cdc3-124 (E43K), wat1-1 (S278F)
normal protein phosphorylation during vegetative growth
affecting nak1cdc3-124 (E43K), cdc16-116, nak1-5A (S479A, S491A, S501A, S533A, S561A)
normal septationcdc3-124 (E43K), cdc16-116, nak1-5A (S479A, S491A, S501A, S533A, S561A)
Target Of
OntologyRelationshipGeneProduct
GO substrate of ubp15 ubiquitin C-terminal hydrolase Ubp15
GO substrate of ubp4 ubiquitin C-terminal hydrolase Ubp4
GO substrate of ubp5 ubiquitin C-terminal hydrolase Ubp5
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2579514..2579466, 2579416..2579084, 2579027..2578956
Intron2579465..2579417, 2579083..2579028
mRNA2579514..2578956
5' UTR2579514..2579479AU008462
CDS2579478..2579466, 2579416..2579084, 2579027..2578990
3' UTR2578989..2578956AU008190
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00235 Pfam IPR005455 Profilin 1 127 1
SM00392 SMART IPR005455 Profilin 1 127 1
PS00414 Prosite Patterns IPR027310 Profilin conserved site 1 8 1
PTHR11604 HMMPANTHER IPR005455 Profilin 1 127 1
3.30.450.30 Gene3D Profilin-2 1 127 2
SSF55770 SuperFamily IPR005455 Profilin 2 127 1
PR01640 PRINTS IPR005455 Profilin 113 126 1
PR01640 PRINTS IPR005455 Profilin 49 63 1
PR01640 PRINTS IPR005455 Profilin 25 38 1
PR01640 PRINTS IPR005455 Profilin 100 113 1
PR01640 PRINTS IPR005455 Profilin 68 77 1
PR01640 PRINTS IPR005455 Profilin 1 12 1
PR00392 PRINTS IPR005455 Profilin 96 109 1
PR00392 PRINTS IPR005455 Profilin 18 27 1
PR00392 PRINTS IPR005455 Profilin 37 57 1
PR00392 PRINTS IPR005455 Profilin 3 12 1
PR00392 PRINTS IPR005455 Profilin 109 126 1
PR00392 PRINTS IPR005455 Profilin 61 75 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 105.60 Da
Charge -0.50
Codon Adaptation Index 0.62
Isoelectric point 5.82
Molecular weight 13.41 kDa
Number of residues 127
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for cdc3 (SPAC4A8.15c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
239543during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
247873during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
48285.67during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
232138during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
60595.21during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
15during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3093
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC4A8.15c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
forms complex withcdc12formin Cdc12 Reconstituted ComplexPMID:18305104
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC4A8.15c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withrad2414-3-3 protein Rad24 Synthetic Growth DefectPMID:16085489
synthetic growth defect withmid1medial ring protein Mid1 Synthetic Growth DefectPMID:18378776
synthetic growth defect withclp1Cdc14-related protein phosphatase Clp1/Flp1 Synthetic Growth DefectPMID:18378776
synthetic growth defect witharc1ARP2/3 actin-organizing complex subunit Sop2 Synthetic Growth DefectPMID:8978670
synthetic growth defect withimp2contractile ring protein Imp2 Synthetic Growth DefectPMID:19139265
synthetic growth defect withact1actin Act1 Synthetic Growth DefectPMID:23589458
synthetic growth defect withrng8contractile ring myosin V regulator Rng8 Synthetic Growth DefectPMID:24798735
synthetic growth defect withblt1ubiquitin domain-like protein Blt1 Synthetic Growth DefectPMID:24790095
synthetically rescued byarp3Arp2/3 protein complex, actin-like protein subunit Arp3 Synthetic RescuePMID:8978671
synthetically rescued byarc1ARP2/3 actin-organizing complex subunit Sop2 Synthetic RescuePMID:8978670
synthetically rescued byarp2ARP2/3 actin-organizing complex subunit Arp2 Synthetic RescuePMID:10588653
synthetically rescued byasp1inositol hexakisphosphate kinase/inositol pyrophosphate synthase (predicted) Synthetic RescuePMID:10388810
rescued bywee1M phase inhibitor protein kinase Wee1 Phenotypic SuppressionPMID:20805322
rescued byact1actin Act1 Phenotypic SuppressionPMID:8207058
rescued byssp1Ca2+/calmodulin-dependent (CaMMK)-like protein kinase Ssp1 Phenotypic SuppressionPMID:10233158
rescued bysid2NDR kinase Sid2 Phenotypic SuppressionPMID:20805322
enhances phenotype ofadf1actin depolymerizing factor, cofilin Phenotypic EnhancementPMID:16467379
enhances phenotype ofacp2F-actin capping protein beta subunit Acp2 Phenotypic EnhancementPMID:16866873
phenotype enhanced bycdc16two-component GAP Cdc16 Phenotypic EnhancementPMID:20805322
phenotype enhanced bysec8exocyst complex subunit Sec8 Phenotypic EnhancementPMID:21148300
synthetic lethal withacp1F-actin capping protein alpha subunit Synthetic LethalityPMID:16866873
synthetic lethal withain1alpha-actinin Synthetic LethalityPMID:11294907
synthetic lethal withacp2F-actin capping protein beta subunit Acp2 Synthetic LethalityPMID:15743909
synthetic lethal withadf1actin depolymerizing factor, cofilin Synthetic LethalityPMID:16467379
synthetic lethal withcdc12formin Cdc12 Synthetic LethalityPMID:9105045
synthetic lethal withact1actin Act1 Synthetic LethalityPMID:23589458
synthetic lethal withpom1DYRK family protein kinase Pom1 Synthetic LethalityPMID:9573052
External References
Database Identifier Description
NBRP SPAC4A8.15c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC4A8.15c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC4A8.15c BioGRID Interaction Datasets
Expression Viewer SPAC4A8.15c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC4A8.15c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC4A8.15c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC4A8.15c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC4A8.15c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC4A8.15c Transcriptome Viewer (Bähler Lab)
GEO SPAC4A8.15c GEO profiles
PInt SPAC4A8.15c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC4A8.15c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC4A8.15c Fission yeast phenotypic data & analysis
Cyclebase SPAC4A8.15c.1 Cell Cycle Data
SPD / RIKEN41/41C06Orfeome Localization Data
UniProtKB/SwissProtP39825Profilin
ModBaseP39825Database of comparative protein structure models
STRINGP39825Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593827profilin
RefSeq mRNANM_001019256972h- profilin (cdc3), mRNA
ePDB3D9YThe European PDB
PDB3D9YPDB
PDBsum3D9YPDBsum
ePDB3DAVThe European PDB
PDB3DAVPDB
PDBsum3DAVPDBsum
European Nucleotide Archive-ENA Protein Mapping
European Nucleotide ArchiveCAB38578.1ENA Protein Mapping
UniParcUPI000013231DUniProt Archive

Literature for cdc3

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016