cdc3 (SPAC4A8.15c)


Gene Standard Namecdc3 Characterisation Statuspublished
Systematic IDSPAC4A8.15c Feature Typeprotein coding
Synonyms Name Description
Productprofilin Product Size127aa, 13.41 kDa
Genomic Location Chromosome I, 2579514-2578956 (559nt); CDS:2579478-2578990 (489nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0003785actin monomer bindingIDAPMID:112949142
GO:0005085guanyl-nucleotide exchange factor activityIDAPMID:1129491436
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0044396actin cortical patch organizationIMPPMID:88347985
GO:0071519actin filament bundle assembly involved in cytokinetic actomyosin contractile ring assemblyNASGO_REF:00000014
GO:0030041actin filament polymerizationIMPPMID:112315725
IDAPMID:24127216
GO:0000915actomyosin contractile ring assemblyIGIact1PMID:820705826
IMPPMID:8834798
IMPPMID:8207058
GO:0032220plasma membrane fusion involved in cytogamyIMPPMID:94906316
GO:0043547positive regulation of GTPase activityIDAPMID:1129491452
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030479actin cortical patchTASPMID:897867047
GO:0051285cell cortex of cell tipIDAPMID:820705841
GO:0005829cytosolIDAPMID:168233722316
GO:0005937mating projectionIDAPMID:94906318
GO:0031097medial cortexIDAPMID:820705833
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopycdc3ΔNullPECO:0000005, PECO:0000137PMID:236978061331
Microscopycdc3ΔNullPMID:20473289
Cell growth assaycdc3-124 (E43K)Not specifiedPECO:0000102, PECO:0000004PMID:8978671
FYPO:0002141normal cell population growth at low temperatureCell growth assaycdc3-124 (E43K)Not specifiedPECO:0000102PMID:89786718

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000791abnormal actin cortical patch morphologyMicroscopycdc3-313Not specifiedPECO:0000004PMID:88347982
FYPO:0000727abnormal actin filament organizationMicroscopycdc3+ (wild type)OverexpressionPMID:820705810
FYPO:0001009abolished actomyosin contractile ring assemblyMicroscopycdc3+ (wild type)OverexpressionPMID:82070583
Microscopycdc3ΔNullPMID:8207058
Microscopycdc3-124 (E43K)EndogenousPECO:0000004PMID:8207058
FYPO:0002555abolished protein localization to medial cortex during vegetative growth8
affecting cdc12Microscopycdc3-313Not specifiedPMID:9105045
FYPO:0002297dispersed actin cortical patch localization during mitotic interphaseMicroscopycdc3-313Not specifiedPECO:0000004PMID:88347983
FYPO:0000133elongated multinucleate vegetative cellMicroscopycdc3-124 (E43K)PECO:0000004PMID:820705826
FYPO:0001011filamentous actin absentMicroscopycdc3ΔNullPMID:82070582
FYPO:0000252increased spontaneous diploidizationOthercdc3-313Not specifiedPMID:883479821
FYPO:0002987inviable after spore germination, without cell division, swollen dumbbell-shaped cell3
expressivity FYPO_EXT:0000003Microscopycdc3ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0000049inviable cellCell growth assaycdc3ΔNullPMID:82070581385
FYPO:0000779inviable elongated dumbbell-shaped cellMicroscopycdc3ΔNullPMID:82070586
Microscopycdc3-124 (E43K)PECO:0000004PMID:8207058
Microscopycdc3+ (wild type)OverexpressionPMID:8207058
FYPO:0002025inviable elongated multinucleate vegetative cell with abnormal septum morphologyMicroscopycdc3-313Not specifiedPECO:0000004PMID:88347982
FYPO:0001006inviable, elongated, multinucleate dumbbell-shaped cellMicroscopycdc3-6Not specifiedPECO:0000004PMID:64907494
Microscopycdc3-124 (E43K)EndogenousPECO:0000004PMID:958201
Microscopycdc3-6EndogenousPECO:0000004PMID:958201
FYPO:0000339mislocalized septumMicroscopycdc3-124 (E43K)PECO:0000004PMID:820705829
FYPO:0001007normal mitosisMicroscopycdc3-124 (E43K)EndogenousPMID:82070588
FYPO:0001587normal protein localization to cell tip14
affecting ags1Microscopycdc3-6Not specifiedPECO:0000004PMID:22891259
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
125795142579466
225794162579084
325790272578956
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00235 Pfam IPR005455 Profilin 2 122 1
SM00392 SMART IPR005455 Profilin 1 127 1
PS00414 Prosite Patterns IPR027310 Profilin conserved site 1 8 1
PTHR11604:SF0 HMMPANTHER 1 127 1
PTHR11604 HMMPANTHER IPR005455 Profilin 1 127 1
3.30.450.30 Gene3D 1 127 2
SSF55770 SuperFamily IPR005455 Profilin 2 127 1
PR01640 PRINTS IPR005455 Profilin 25 38 1
PR01640 PRINTS IPR005455 Profilin 49 63 1
PR01640 PRINTS IPR005455 Profilin 113 126 1
PR01640 PRINTS IPR005455 Profilin 100 113 1
PR01640 PRINTS IPR005455 Profilin 68 77 1
PR01640 PRINTS IPR005455 Profilin 1 12 1
PR00392 PRINTS IPR005455 Profilin 18 27 1
PR00392 PRINTS IPR005455 Profilin 37 57 1
PR00392 PRINTS IPR005455 Profilin 61 75 1
PR00392 PRINTS IPR005455 Profilin 3 12 1
PR00392 PRINTS IPR005455 Profilin 109 126 1
PR00392 PRINTS IPR005455 Profilin 96 109 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 105.60 Da
Charge -0.50
Isoelectric point 5.82
Molecular weight 13.41 kDa
Number of residues 127
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
239543during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
247873during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
232138during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
48285.67during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
60595.21during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
15during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Synthetic Growth DefectPMID:23297348
acp1F-actin capping protein alpha subunit Synthetic LethalityPMID:16866873
acp2F-actin capping protein beta subunit Acp2 Synthetic LethalityPMID:16866873
Phenotypic Enhancement
Synthetic LethalityPMID:15743909
rng8contractile ring myosin V regulator Rng8 Synthetic Growth DefectPMID:24798735
arp3Arp2/3 protein complex, actin-like protein subunit Arp3 Phenotypic SuppressionPMID:8978671
arp2ARP2/3 actin-organizing complex subunit Arp2 Synthetic RescuePMID:10588653
ssp1Ca2+/calmodulin-dependent (CaMMK)-like protein kinase Ssp1 Phenotypic SuppressionPMID:10233158
cdc16two-component GAP Cdc16 Phenotypic EnhancementPMID:20805322
adf1actin depolymerizing factor, cofilin Synthetic LethalityPMID:16467379
Phenotypic Enhancement
sid2NDR kinase Sid2 Phenotypic SuppressionPMID:20805322
imp2contractile ring protein Imp2 Synthetic Growth DefectPMID:19139265
sec8exocyst complex subunit Sec8 Phenotypic EnhancementPMID:21148300
cdc12formin Cdc12 Synthetic LethalityPMID:9105045
arc1ARP2/3 actin-organizing complex subunit Sop2 Synthetic Growth DefectPMID:8978670
Synthetic Rescue
ain1alpha-actinin Synthetic LethalityPMID:11294907
pom1DYRK family protein kinase Pom1 Synthetic LethalityPMID:9573052
asp1inositol hexakisphosphate kinase/inositol pyrophosphate synthase (predicted) Synthetic RescuePMID:10388810
blt1Blt1 Synthetic Growth DefectPMID:24790095
act1actin Act1 Synthetic Growth DefectPMID:10371213
Synthetic Growth DefectPMID:23589458
Synthetic Lethality
Phenotypic SuppressionPMID:8207058
wee1M phase inhibitor protein kinase Wee1 Phenotypic SuppressionPMID:20805322
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
cdc12formin Cdc12 Reconstituted ComplexPMID:18305104
Reconstituted ComplexPMID:9105045
External References
Database Identifier Description
NBRP SPAC4A8.15c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC4A8.15c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC4A8.15c BioGRID Interaction Datasets
Expression Viewer SPAC4A8.15c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC4A8.15c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC4A8.15c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC4A8.15c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC4A8.15c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC4A8.15c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC4A8.15c Cell Cycle Data
GEO SPAC4A8.15c GEO profiles
PInt SPAC4A8.15c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC4A8.15c Peptides identified in tandem mass spectrometry proteomics experiments
WikiGene2543526profilin
EntrezGene2543526profilin
SPD / RIKEN41/41C06Orfeome Localization Data
UniProtKB/SwissProtP39825Profilin
ModBaseP39825Database of comparative protein structure models
STRINGP39825Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593827profilin
RefSeq mRNANM_001019256972h- profilin (cdc3), mRNA
ePDB3D9YThe European PDB
PDB3D9YPDB
PDBsum3D9YPDBsum
ePDB3DAVThe European PDB
PDB3DAVPDB
PDBsum3DAVPDBsum
European Nucleotide Archive-ENA Protein Mapping
European Nucleotide ArchiveCAB38578ENA Protein Mapping
European Nucleotide ArchiveCAB38578.1ENA Protein Mapping
UniParcUPI000013231DUniProt Archive

Literature for cdc3

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014