ade4 (SPAC4D7.08c)

Gene Standard Nameade4 Characterisation Statuspublished
Systematic IDSPAC4D7.08c Feature Typeprotein coding
Synonymsaza1, min13 Name Description
Productamidophosphoribosyltransferase Ade4 Product Size533aa, 59.83 kDa
Genomic Location Chromosome I, 5366539-5363988 (2552nt); CDS:5365961-5364360 (1602nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
amidophosphoribosyltransferase activity1
Annotation ExtensionEvidenceWith/FromReference
metal ion binding743
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
'de novo' IMP biosynthetic process8
Annotation ExtensionEvidenceWith/FromReference
adenine metabolic process7
Annotation ExtensionEvidenceWith/FromReference
purine nucleobase biosynthetic process12
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Single-allele Phenotypes

Population Phenotype

Term NameGenotypesCount
growth auxotrophic for adenineade4-243
viable vegetative cell populationade4Δ3815

Cell Phenotype

Term NameGenotypesCount
decreased GMP bindingaza-11
decreased IMP bindingaza-11
increased 5-phosphoribose 1-diphosphate bindingaza-11
increased acid phosphatase activityade4-3110
increased RNA level during cellular response to adenine starvation3
affecting rds1ade4-31
inviable swollen elongated cell with enlarged nucleus upon entry into stationary phaseade4-6
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons5366539..5366340, 5365964..5363988
5' UTR5366539..5366340, 5365964..5365962PMID:21511999
3' UTR5364359..5363988AU011122
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13522 Pfam 69 212 2
PF00156 Pfam IPR000836 Phosphoribosyltransferase domain 299 411 4
PS51278 Prosite Profiles IPR017932 Glutamine amidotransferase type 2 domain 2 238 5
PTHR11907 HMMPANTHER 1 500 1
PTHR11907:SF8 HMMPANTHER 1 500 1 Gene3D IPR029057 Phosphoribosyltransferase-like 286 461 11 Gene3D IPR029055 Nucleophile aminohydrolases, N-terminal 2 239 19
SSF53271 SuperFamily IPR029057 Phosphoribosyltransferase-like 267 491 11
SSF56235 SuperFamily IPR029055 Nucleophile aminohydrolases, N-terminal 2 241 22
MF_01931 hamap IPR005854 Amidophosphoribosyltransferase 1 482 1
PIRSF000485 PIRSF IPR005854 Amidophosphoribosyltransferase 1 496 1
TIGR01134 tigrfam IPR005854 Amidophosphoribosyltransferase 2 481 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.26 Da
Charge -3.50
Codon Adpatation Index 0.55
Isoelectric point 6.13
Molecular weight 59.83 kDa
Number of residues 533

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS506 1672
present during mitotic M phaseS520
present during cellular response to thiabendazoleS506
Annotation ExtensionEvidenceResidueReference
experimental evidence S506 PMID:24763107
present during cellular response to thiabendazole IDA S506 PMID:18257517
present during mitotic M phase experimental evidence S520 PMID:21712547
O-phospho-L-threonine 699
present during mitotic M phaseT524
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T524 PMID:21712547
phosphorylated residue 1930
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Gene Expression

Qualitative Gene Expression

RNA levelpresent during GO:0072690Northern assay evidencePMID:8082193

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
42654during GO:0000080PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
39068during GO:0000084PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
37949during GO:0000085PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
36598during GO:0000087PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
39075during GO:0072690PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
25306.4during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
10067.36during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
15during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
3.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
predominantly single copy (one to one)3090
conserved in fungi4604
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3421
conserved in vertebrates3396

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
affinity captured bycbs2AMP-activated protein kinase gamma subunit cbs2 Affinity Capture-MSPMID:19371376
affinity captured byssp2AMP-activated protein serine/threonine kinase alpha subunit Ssp2 Affinity Capture-MSPMID:19371376
External References
Database Identifier Description
NBRP SPAC4D7.08c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC4D7.08c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC4D7.08c BioGRID Interaction Datasets
Expression Viewer SPAC4D7.08c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC4D7.08c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC4D7.08c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC4D7.08c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC4D7.08c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC4D7.08c Transcriptome Viewer (Bähler Lab)
GEO SPAC4D7.08c GEO profiles
PInt SPAC4D7.08c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC4D7.08c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC4D7.08c Fission yeast phenotypic data & analysis
Cyclebase SPAC4D7.08c.1 Cell Cycle Data
SPD / RIKEN25/25F11Orfeome Localization Data
IntEnz2.4.2.14Integrated relational Enzyme database
Rhea2.4.2.14Annotated reactions database
ModBaseP41390Database of comparative protein structure models
STRINGP41390Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594961amidophosphoribosyltransferase Ade4
European Nucleotide ArchiveCU329670ENA EMBL mapping
European Nucleotide ArchiveX72293ENA EMBL mapping
MEROPSC44.001MEROPS - the Peptidase Database
European Nucleotide ArchiveCAA51034ENA Protein Mapping
European Nucleotide ArchiveCAA51034.1ENA Protein Mapping
European Nucleotide ArchiveCAB11280ENA Protein Mapping
European Nucleotide ArchiveCAB11280.1ENA Protein Mapping
MetaCycPWY-61215-aminoimidazole ribonucleotide biosynthesis I
MetaCycPWY-61225-aminoimidazole ribonucleotide biosynthesis II
MetaCycPWY-6277Superpathway of 5-aminoimidazole ribonucleotide biosynthesis
MetaCycPWY-72824-amino-2-methyl-5-phosphomethylpyrimidine biosynthesis (yeast)
KEGG00230+ metabolism
KEGG00250+, aspartate and glutamate metabolism
UniParcUPI0000132A13UniProt Archive
UniPathwayUPA00074Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2

Literature for ade4

Search: Europe PMC or PubMed

Release Version: PomBase:28_55 - 09 Sep 2015