ade4 (SPAC4D7.08c)


Gene Standard Nameade4 Characterisation Statuspublished
Systematic IDSPAC4D7.08c Feature Typeprotein coding
Synonymsaza1, min13 Name Description
Productamidophosphoribosyltransferase Ade4 Product Size533aa, 59.83 kDa
Genomic Location Chromosome I, 5366539-5363988 (2552nt); CDS:5365961-5364360 (1602nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004044amidophosphoribosyltransferase activityIEA EC:2.4.2.14GO_REF:00000031
GO:0046872metal ion bindingIEAUniProtKB-KW:KW-0479GO_REF:0000037748
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006189'de novo' IMP biosynthetic processISOSGD:S000004915GO_REF:00000248
GO:0046083adenine metabolic processIMPPMID:80821938
GO:0006541glutamine metabolic processIEAUniProtKB-KW:KW-0315GO_REF:000003719
GO:0009113purine nucleobase biosynthetic processIEAIPR005854GO_REF:000000213
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmISSUniProtKB:P04046GO_REF:00000014204
IDAPMID:16823372
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000747growth auxotrophic for adenineCell growth assayade4-315Not specifiedPMID:80821932
Cell growth assayade4-51Not specifiedPMID:8082193
Cell growth assayade4-250Not specifiedPMID:8082193
Cell growth assayade4-24Not specifiedPMID:8082193
Cell growth assayade4-31Not specifiedPMID:8082193
FYPO:0002060viable vegetative cell populationMicroscopyade4ΔNullPMID:205199593751

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002311decreased GMP bindingEnzyme assay dataaza-1Not specifiedPMID:43142331
FYPO:0002309decreased IMP bindingEnzyme assay dataaza-1Not specifiedPMID:43142331
FYPO:0002307increased 5-phosphoribose 1-diphosphate bindingEnzyme assay dataaza-1Not specifiedPMID:43142331
FYPO:0001242increased RNA level during cellular response to adenine starvation3
affecting rds1Northern assay evidenceade4-31EndogenousPECO:0000126, PECO:0000021PMID:7565608
FYPO:0002243increased acid phosphatase activitySubstance quantification evidenceade4-31Not specifiedPECO:0000137PMID:139451010
FYPO:0002728inviable swollen elongated cell with enlarged nucleus upon entry into stationary phaseMicroscopyade4-Not specifiedPMID:27434316
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
153665395366340
253659645363988
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00156 Pfam IPR000836 Phosphoribosyltransferase domain 287 410 4
PF00310 Pfam IPR000583 Class II glutamine amidotransferase domain 55 212 3
PS51278 Prosite Profiles IPR017932 Glutamine amidotransferase type 2 domain 2 238 5
PTHR11907 HMMPANTHER 1 503 1
PTHR11907:SF0 HMMPANTHER 1 503 1
3.60.20.10 Gene3D 2 239 19
3.40.50.2020 Gene3D 286 461 11
SSF53271 SuperFamily Phosphoribosyltransferase-like 267 491 11
SSF56235 SuperFamily Nucleophile aminohydrolases, N-terminal 2 241 22
PIRSF000485 PIRSF IPR005854 Amidophosphoribosyl transferase 1 495 1
TIGR01134 tigrfam IPR005854 Amidophosphoribosyl transferase 2 481 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.26 Da
Charge -3.50
Isoelectric point 6.13
Molecular weight 59.83 kDa
Number of residues 533
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS520PMID:217125471670
present during mitotic M phase
present during cellular response to thiabendazoleIDAS506PMID:18257517
experimental evidenceS506PMID:24763107
MOD:00047O-phospho-L-threonineexperimental evidenceT524PMID:21712547690
present during mitotic M phase
MOD:00696phosphorylated residueNASPMID:195477441922
NASPMID:18257517
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelpresent during GO:0072690Northern assay evidencePMID:8082193

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
42654during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
39068during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
37949during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
36598during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
39075during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
25306.4during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
10067.36during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
15during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
3.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in bacteria1002
conserved in metazoa3428
conserved in vertebrates3402
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
cbs2protein kinase activator (predicted) Affinity Capture-MSPMID:19371376
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
ssp2serine/threonine protein kinase Ssp2 Affinity Capture-MSPMID:19371376
External References
Database Identifier Description
NBRP SPAC4D7.08c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC4D7.08c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC4D7.08c BioGRID Interaction Datasets
Expression Viewer SPAC4D7.08c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC4D7.08c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC4D7.08c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC4D7.08c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC4D7.08c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC4D7.08c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC4D7.08c Cell Cycle Data
GEO SPAC4D7.08c GEO profiles
PInt SPAC4D7.08c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC4D7.08c Peptides identified in tandem mass spectrometry proteomics experiments
IntEnz2.4.2.14Integrated relational Enzyme database
Rhea2.4.2.14Annotated reactions database
SPD / RIKEN25/25F11Orfeome Localization Data
UniProtKB/SwissProtP41390Amidophosphoribosyltransferase
ModBaseP41390Database of comparative protein structure models
STRINGP41390Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594961amidophosphoribosyltransferase Ade4
RefSeq mRNANM_001020392972h- amidophosphoribosyltransferase Ade4 (ade4), mRNA
European Nucleotide ArchiveCAA51034.1ENA Protein Mapping
European Nucleotide ArchiveCAB11280.1ENA Protein Mapping
MetaCycPWY-61215-aminoimidazole ribonucleotide biosynthesis I
MetaCycPWY-61225-aminoimidazole ribonucleotide biosynthesis II
MetaCycPWY-6277Superpathway of 5-aminoimidazole ribonucleotide biosynthesis
MetaCycPWY-72824-amino-2-methyl-5-phosphomethylpyrimidine biosynthesis (yeast)
UniParcUPI0000132A13UniProt Archive
UniPathwayUPA00074Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2

Literature for ade4

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014