ade4 (SPAC4D7.08c)

Gene Standard Nameade4 Characterisation Statuspublished
Systematic IDSPAC4D7.08c Feature Typeprotein coding
Synonymsaza1, min13 Name Description
Productamidophosphoribosyltransferase Ade4 Product Size533aa, 59.83 kDa
Genomic Location Chromosome I, 5366539-5363988 (2552nt); CDS:5365961-5364360 (1602nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004044amidophosphoribosyltransferase activityIEA EC:
GO:0046872metal ion bindingIEAUniProtKB-KW:KW-0479GO_REF:0000037747
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006189'de novo' IMP biosynthetic processISOSGD:S000004915GO_REF:00000248
GO:0046083adenine metabolic processIMPPMID:80821938
GO:0006541glutamine metabolic processIEAUniProtKB-KW:KW-0315GO_REF:000003719
GO:0009113purine nucleobase biosynthetic processIEAIPR005854GO_REF:000000212
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000747growth auxotrophic for adenineCell growth assayade4-51Not specifiedPMID:80821932
Cell growth assayade4-250Not specifiedPMID:8082193
Cell growth assayade4-24Not specifiedPMID:8082193
Cell growth assayade4-31Not specifiedPMID:8082193
Cell growth assayade4-315Not specifiedPMID:8082193
FYPO:0002060viable vegetative cell populationade4ΔNullPMID:205199593755

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002311decreased GMP bindingEnzyme assay dataaza-1Not specifiedPMID:43142331
FYPO:0002309decreased IMP bindingEnzyme assay dataaza-1Not specifiedPMID:43142331
FYPO:0002307increased 5-phosphoribose 1-diphosphate bindingEnzyme assay dataaza-1Not specifiedPMID:43142331
FYPO:0001242increased RNA level during cellular response to adenine starvationNorthern assay evidenceade4-31EndogenousPECO:0000126, PECO:0000021PMID:75656083
affecting rds1
FYPO:0002728inviable swollen elongated cell with enlarged nucleus upon entry into stationary phaseMicroscopyade4-Not specifiedPMID:27434316
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Start End Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00310 Pfam IPR000583 Class II glutamine amidotransferase domain 55 212 3
PF00156 Pfam IPR000836 Phosphoribosyltransferase domain 287 410 4
PS51278 Prosite Profiles IPR017932 Glutamine amidotransferase type 2 domain 2 238 5
PTHR11907:SF0 HMMPANTHER 1 503 1
PTHR11907 HMMPANTHER 1 503 1
G3DSA: Gene3D 2 239 19
G3DSA: Gene3D 286 461 11
SSF56235 SuperFamily 2 241 22
SSF53271 SuperFamily 267 491 11
Low complexity (SEG) seg 321 334
PIRSF000485 PIRSF IPR005854 Amidophosphoribosyl transferase 1 495 1
TIGR01134 tigrfam IPR005854 Amidophosphoribosyl transferase 2 481 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.26 Da
Charge -3.50
Isoelectric point 6.13
Molecular weight 59.83 kDa
Number of residues 533

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residuePMID:182575171192
Gene Expression

Qualitative Gene Expression

RNA levelpresentNorthern assay evidencePMID:8082193
during GO:0072690

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
10067.36during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
25306.4during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
15during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
3.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3394

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
cbs2protein kinase activator (predicted) Affinity Capture-MSPMID:19371376
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
ssp2serine/threonine protein kinase Ssp2 Affinity Capture-MSPMID:19371376
External References
Database Identifier Description
NBRP SPAC4D7.08c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC4D7.08c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC4D7.08c BioGRID Interaction Datasets
Expression Viewer SPAC4D7.08c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC4D7.08c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC4D7.08c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC4D7.08c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC4D7.08c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC4D7.08c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC4D7.08c Cell Cycle Data
GEO SPAC4D7.08c GEO profiles
PInt SPAC4D7.08c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2543626amidophosphoribosyltransferase Ade4
EntrezGene2543626amidophosphoribosyltransferase Ade4
IntEnz2.4.2.14Integrated relational Enzyme database
Rhea2.4.2.14Annotated reactions database
SPD / RIKEN25/25F11Orfeome Localization Data
ModBaseP41390Database of comparative protein structure models
StringP41390Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594961amidophosphoribosyltransferase Ade4
RefSeq mRNANM_001020392972h- amidophosphoribosyltransferase Ade4 (ade4), mRNA
European Nucleotide ArchiveCAA51034ENA Protein Mapping
European Nucleotide ArchiveCAB11280ENA Protein Mapping
UniParcUPI0000132A13UniProt Archive

Literature for ade4

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014