ade4 (SPAC4D7.08c)

Gene Standard Nameade4 Characterisation Statuspublished
Systematic IDSPAC4D7.08c Feature Typeprotein coding
Synonymsaza1, min13 Name Description
Productamidophosphoribosyltransferase Ade4 Product Size533aa, 59.83 kDa
Genomic Location Chromosome I, 5366539-5363988 (2552nt); CDS:5365961-5364360 (1602nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
amidophosphoribosyltransferase activity1
Annotation ExtensionEvidenceWith/FromReference
metal ion binding750
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
'de novo' IMP biosynthetic process8
Annotation ExtensionEvidenceWith/FromReference
adenine metabolic process7
Annotation ExtensionEvidenceWith/FromReference
purine nucleobase biosynthetic process12
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
growth auxotrophic for adenineade4-2503
viable vegetative cell populationade4Δ3850

Cell Phenotype

Term NameGenotypesCount
decreased GMP bindingaza-11
decreased IMP bindingaza-11
increased 5-phosphoribose 1-diphosphate bindingaza-11
increased acid phosphatase activityade4-3110
increased cellular pigment accumulation31
colour PATO:0000323min13-32
colour PATO:0000323ade4-31
increased RNA level during cellular response to adenine starvation3
affecting rds1ade4-31
inviable swollen elongated cell with enlarged nucleus upon entry into stationary phaseade4-6
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons5366539..5366340, 5365964..5363988
5' UTR5366539..5366340, 5365964..5365962PMID:21511999
3' UTR5364359..5363988AU011122
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00156 Pfam IPR000836 Phosphoribosyltransferase domain 299 411 4
PF13522 Pfam 69 212 2
PS51278 Prosite Profiles IPR017932 Glutamine amidotransferase type 2 domain 2 238 5
PTHR11907:SF0 HMMPANTHER 1 494 1
PTHR11907 HMMPANTHER 1 494 1 Gene3D IPR029057 Phosphoribosyltransferase-like 286 461 11 Gene3D IPR029055 Nucleophile aminohydrolases, N-terminal 2 239 19
SSF56235 SuperFamily IPR029055 Nucleophile aminohydrolases, N-terminal 2 241 22
SSF53271 SuperFamily IPR029057 Phosphoribosyltransferase-like 267 491 11
PIRSF000485 PIRSF IPR005854 Amidophosphoribosyltransferase 1 496 1
MF_01931 HAMAP IPR005854 Amidophosphoribosyltransferase 1 482 1
TIGR01134 TIGRFAM IPR005854 Amidophosphoribosyltransferase 2 481 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.26 Da
Charge -3.50
Codon Adaptation Index 0.55
Isoelectric point 6.13
Molecular weight 59.83 kDa
Number of residues 533

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during cellular response to thiabendazoleS506
present during mitotic M phaseS520
S506, S526
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S506 PMID:18257517
present during mitotic M phase experimental evidence S520 PMID:21712547
IDA S526 PMID:25720772
experimental evidence S506 PMID:24763107
IDA S506 PMID:25720772
O-phospho-L-threonine 1085
present during mitotic M phaseT524
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T524 PMID:21712547
IDA T524 PMID:25720772
phosphorylated residue 2511
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
O-phosphorylated residueS520,S526 2457
Annotation ExtensionEvidenceResidueReference
IDA S520,S526 PMID:25720772
Gene Expression

Qualitative Gene Expression

RNA levelpresent during GO:0072690Northern assay evidencePMID:8082193

Quantitative Gene Expression

View graphical display of gene expression data for ade4 (SPAC4D7.08c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
42654during GO:0000080PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
39068during GO:0000084PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
37949during GO:0000085PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
36598during GO:0000087PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
25306.4during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
39075during GO:0072690PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
10067.36during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
15during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
3.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
predominantly single copy (one to one)3093
conserved in fungi4607
conserved in eukaryotes4517
conserved in bacteria1005
conserved in metazoa3427
conserved in vertebrates3402

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC4D7.08c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bycbs2AMP-activated protein kinase gamma subunit cbs2 Affinity Capture-MSPMID:19371376
affinity captured byssp2AMP-activated protein serine/threonine kinase alpha subunit Ssp2 Affinity Capture-MSPMID:19371376
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
binds activation domain construct withcaf4CCR4-Not complex subunit Caf4/Mdv1 (predicted) Two-hybridPMID:26771498
binds activation domain construct withatg11autophagy protein Atg11 Two-hybridPMID:26771498
binds activation domain construct withade4amidophosphoribosyltransferase Ade4 Two-hybridPMID:26771498
External References
Database Identifier Description
NBRP SPAC4D7.08c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC4D7.08c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC4D7.08c BioGRID Interaction Datasets
Expression Viewer SPAC4D7.08c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC4D7.08c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC4D7.08c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC4D7.08c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC4D7.08c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC4D7.08c Transcriptome Viewer (Bähler Lab)
GEO SPAC4D7.08c GEO profiles
PInt SPAC4D7.08c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC4D7.08c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC4D7.08c Fission yeast phenotypic data & analysis
Cyclebase SPAC4D7.08c.1 Cell Cycle Data
IntEnz2.4.2.14Integrated relational Enzyme database
Rhea2.4.2.14Annotated reactions database
SPD / RIKEN25/25F11Orfeome Localization Data
ModBaseP41390Database of comparative protein structure models
STRINGP41390Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594961amidophosphoribosyltransferase Ade4
European Nucleotide ArchiveCAA51034.1ENA Protein Mapping
European Nucleotide ArchiveCAB11280.1ENA Protein Mapping
MetaCycPWY-61215-aminoimidazole ribonucleotide biosynthesis I
MetaCycPWY-61225-aminoimidazole ribonucleotide biosynthesis II
MetaCycPWY-6277Superpathway of 5-aminoimidazole ribonucleotide biosynthesis
MetaCycPWY-72824-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis (yeast)
KEGG00230+ metabolism
KEGG00250+, aspartate and glutamate metabolism
UniParcUPI0000132A13UniProt Archive

Literature for ade4

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016