wsp1 (SPAC4F10.15c)

Gene Standard Namewsp1 ChromosomeI
Systematic IDSPAC4F10.15c Gene Start4863516
Synonyms Gene End4861253
ProductWASp homolog Gene Length2264
Feature Typeprotein coding CDS Start4863448
Name Description CDS End4861530
Characterisation Statuspublished CDS Length1919

Ensembl Gene Location
  1. spn1
  2. fta1
  3. mpd2
  4. SPNCRNA.1030
  5. btf3
  6. wsp1
  7. SPNCRNA.1031
  8. SPAC4F10.16c
  9. SPAC4F10.17
  10. SPNCRNA.254
  11. SPNCRNA.1032
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000422decreased endocytosisMicroscopywsp1delta (deletion)deletionPMID:21620704
FYPO:0000427abnormal G1 to G0 transitionCell growth assaywsp1-318 (W44R)amino_acid_mutationPECO:0000090, PECO:0000005PMID:19366728
FYPO:0000728abnormal actin cortical patch assemblyMicroscopywsp1-318 (W44R)amino_acid_mutationPECO:0000014PMID:19366728
FYPO:0000746abolished actin cortical patch internalizationMicroscopywsp1delta (deletion)deletionPMID:21620704
FYPO:0001000normal cell cycle arrest in mitotic G1 phase during nitrogen starvationFlow cytometry datawsp1-318 (W44R)amino_acid_mutationPECO:0000090, PECO:0000005PMID:19366728
FYPO:0001004viable upon G0 to G1 transitionMicroscopyend4-507 (G73D)amino_acid_mutationPECO:0000095, PECO:0000025PMID:19366728
FYPO:0002060viable vegetative cell populationMicroscopywsp1delta (deletion)deletionPMID:20473289
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0003779actin bindingIEAUniProtKB:KW-0009GO_REF:0000037
GO:0005515protein bindingIPIhob1PMID:15615784
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0000147actin cortical patch assemblyIGImyo1PMID:11076964
different_pathwayIGImyo1PMID:16087707
GO:0000915cytokinesis, actomyosin contractile ring assemblyIMPPMID:12214236
GO:0000916actomyosin contractile ring contractionIMPPMID:12214236
GO:0006897endocytosisIMPPMID:21620704
GO:0006995cellular response to nitrogen starvationIMPPMID:19366728
GO:0009272fungal-type cell wall biogenesisIMPPMID:11076964
GO:0030041actin filament polymerizationIMPPMID:12214236
GO:0044397actin cortical patch internalizationIMPPMID:21620704
GO:0045010actin nucleationIDAPMID:16087707
different_pathwayIGImyo1PMID:16087707
GO:0070314G1 to G0 transition
happens_during cellular response to nitrogen starvationIMPPMID:19366728
GO:0071963establishment or maintenance of cell polarity regulating cell shapeIGInak1PMID:15615784
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0005826actomyosin contractile ringTASPMID:15615784
GO:0030479actin cortical patchIDAPMID:21620704
ISSUniProtKB:Q12446GO_REF:0000001
GO:0051286cell tipTASPMID:15615784
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
148635164863233
248631474861807
348617564861624
448615644861253

References

Region Start End Reference
three_prime_UTR48615294861253PMID:21511999
five_prime_UTR48635164863449PMID:21511999
three_prime_UTR48615294861253PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF00568 IPR000697 Pfam EVH1 21 124 1
SM00461 IPR000697 SMART EVH1 20 127 1
PS50229 IPR000697 Prosite Profiles EVH1 19 130 1
PS51082 IPR003124 Prosite Profiles WH2 domain 499 518 3
G3DSA:2.30.29.30 IPR011993 Gene3D 3 126 35
SSF50729 SuperFamily 19 125 36
PTHR23202 hmmpanther 8 517 2
PTHR23202:SF2 hmmpanther 8 517 1

View domain organization at Pfam

Protein Modifications (PSI-MOD)

Term IDTerm NameEvidenceResidueReference
MOD:00696phosphorylated residuePMID:18257517
PMID:19547744

Protein Properties

Ave. residue weight 103.84 Da
Charge 11.50
Isoelectric point 9.65
Molecular weight 59.61 kDa
Number of residues 574
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
promoter contains homolD box
protein levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide3702.19PMID:23101633
experimental evidencePECO:0000014,
PECO:0000005		population_wide3739.33PMID:23101633
RNA levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide1.6PMID:23101633
experimental evidencePECO:0000014,
PECO:0000005		population_wide6.7PMID:23101633
Disease Association
DescriptionQualifierReference
cancercancer_census
Wiskott-Aldrich syndrome
Species Distribution
DescriptionQualifierReference
predominantly single copy (one to one)
conserved in fungi
conserved in eukaryotes
conserved in metazoa
conserved in vertebrates
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
ubp9ubiquitin C-terminal hydrolase Ubp9 Synthetic Growth DefectPMID:20838651
Synthetic Lethality
bzz1diacylglycerol binding protein Bzz1 (predicted) Synthetic RescuePMID:21885283
nak1PAK-related kinase Nak1 Dosage LethalityPMID:15615784
dip1WISH/DIP/SPIN90 ortholog Dip1 Synthetic Growth DefectPMID:21620704
Phenotypic Enhancement
Synthetic Lethality
myo1myosin type I Synthetic LethalityPMID:11076964
Synthetic LethalityPMID:21885283
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
SPAC10F6.08cHMG box protein Affinity Capture-RNAPMID:19805578
hob1BAR adaptor protein Hob1 Affinity Capture-WesternPMID:15615784
myo1myosin type I Two-hybridPMID:12939254
Reconstituted Complex
Affinity Capture-RNAPMID:19805578
vrp1verprolin Co-localizationPMID:16087707
Two-hybridPMID:12939254
Reconstituted Complex
nak1PAK-related kinase Nak1 Co-localizationPMID:15615784
Affinity Capture-Western
arp2ARP2/3 actin-organizing complex subunit Arp2 Reconstituted ComplexPMID:23148219
arp3actin-like protein Arp3 Reconstituted ComplexPMID:23148219
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External References
Database Identifier Description
NBRP SPAC4F10.15c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC4F10.15c Retrieval of eukaryotic orthologs
BioGrid SPAC4F10.15c BioGRID Interaction Datasets
Bähler Lab SPAC4F10.15c Cell Cycle Expression Profile
Bähler Lab SPAC4F10.15c Meiosis/Sporulation Expression Profies
Bähler Lab SPAC4F10.15c Pheromone response/mating expression profiles
Bähler Lab SPAC4F10.15c Environmental stress expression profiles
Bähler Lab SPAC4F10.15c Bähler Lab Transcriptome Viewer
Cyclebase SPAC4F10.15c Cell Cycle Data
PInt SPAC4F10.15c Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPAC4F10.15c Entrez Gene
WikiGene2543589actin assembly factor
Uniprot_genenameWSP1
EntrezGene2543589actin assembly factor
UniProtKB/SwissProtO36027Wiskott-Aldrich syndrome protein homolog 1
ModBaseO36027Database of comparative protein structure models
Pfam Protein DomainsO36027Pfam Domain Arrangement
RefSeq PeptideNP_594758actin assembly factor
RefSeq mRNANM_001020185972h- actin assembly factor (wsp1), mRNA
European Nucleotide ArchiveAAB92587ENA Protein Mapping
European Nucleotide ArchiveCAB11718ENA Protein Mapping
SPD / RIKEN26/26G03Orfeome Localization Data
UniParcUPI000006C8BEUniProt Archive

Literature for wsp1
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013