SPAC4F10.16c


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPAC4F10.16c Feature Typeprotein coding
Synonyms Name Description
ProductP-type ATPase (predicted) Product Size1367aa, 154.28 kDa
Genomic Location Chromosome I, 4869888-4863739 (6150nt); CDS:4868110-4863961 (4150nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037558
GO:0000287magnesium ion bindingIEAIPR006539GO_REF:000000250
GO:0004012phospholipid-translocating ATPase activityISOSGD:S000000968GO_REF:00000246
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006897endocytosisISOSGD:S000000968GO_REF:000002476
GO:0006886intracellular protein transportISOSGD:S000000968GO_REF:0000024338
GO:0045332phospholipid translocationISOSGD:S000000968GO_REF:00000245
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005783endoplasmic reticulumIDAPMID:16823372594
GO:0016021integral component of membraneIEAUniProtKB-KW:KW-0812GO_REF:00000371018
GO:0005886plasma membraneISOSGD:S000000968GO_REF:0000024247
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopySPAC4F10.16cΔNullPECO:0000005, PECO:0000137PMID:236978063759
MicroscopySPAC4F10.16cΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001MicroscopySPAC4F10.16cΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
148698884864584
248645374863739

UTRs

Region Coordinates Reference
five_prime_UTR4869888..4868111PMID:21511999
three_prime_UTR4863960..4863739PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF12710 Pfam 597 1044 8
PF00122 Pfam IPR008250 P-type ATPase, A domain 298 560 14
TMhelix TMHMM 1241 1260 959
TMhelix TMHMM 1127 1145 959
TMhelix TMHMM 1280 1302 959
TMhelix TMHMM 485 507 959
TMhelix TMHMM 1093 1112 959
TMhelix TMHMM 532 554 959
TMhelix TMHMM 1058 1080 959
TMhelix TMHMM 1212 1234 959
TMhelix TMHMM 1175 1197 959
PS00154 Prosite Patterns IPR018303 P-type ATPase, phosphorylation site 600 606 14
PTHR24092:SF14 HMMPANTHER IPR026871 Phospholipid-transporting P-type ATPase, fungal 18 1320 2
PTHR24092 HMMPANTHER IPR006539 Cation-transporting P-type ATPase, subfamily IV 18 1320 5
2.70.150.10 Gene3D IPR008250 P-type ATPase, A domain 433 469 12
2.70.150.10 Gene3D IPR008250 P-type ATPase, A domain 155 223 12
3.40.1110.10 Gene3D IPR023299 P-type ATPase, cytoplasmic domain N 690 878 13
3.40.1110.10 Gene3D IPR023299 P-type ATPase, cytoplasmic domain N 590 635 13
3.40.50.1000 Gene3D IPR023214 HAD-like domain 1004 1089 33
3.40.50.1000 Gene3D IPR023214 HAD-like domain 879 935 33
2.70.150.10 Gene3D IPR008250 P-type ATPase, A domain 318 381 12
SSF56784 SuperFamily IPR023214 HAD-like domain 976 1081 41
0049471 SuperFamily 428 468 14
0049471 SuperFamily 316 379 14
SSF56784 SuperFamily IPR023214 HAD-like domain 864 927 41
0049473 SuperFamily 1082 1310 14
0049473 SuperFamily 126 215 14
SSF81660 SuperFamily IPR023299 P-type ATPase, cytoplasmic domain N 681 847 11
0049473 SuperFamily 470 592 14
SSF81660 SuperFamily IPR023299 P-type ATPase, cytoplasmic domain N 605 631 11
PR00119 PRINTS IPR001757 Cation-transporting P-type ATPase 598 612 14
PR00119 PRINTS IPR001757 Cation-transporting P-type ATPase 1033 1052 14
TIGR01652 tigrfam IPR006539 Cation-transporting P-type ATPase, subfamily IV 128 1317 5
TIGR01494 tigrfam IPR001757 Cation-transporting P-type ATPase 1003 1114 14

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 112.86 Da
Charge 4.00
Isoelectric point 6.71
Molecular weight 154.28 kDa
Number of residues 1367
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
190during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
192during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
167during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.1during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.82during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
tom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
raf2Rik1-associated factor Raf2 Positive GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
elp2elongator complex subunit Elp2 (predicted) Positive GeneticPMID:22681890
top1DNA topoisomerase I Positive GeneticPMID:22681890
SPBC660.05WW domain containing conserved fungal protein Positive GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
crp79poly(A) binding protein Crp79 Affinity Capture-RNAPMID:20531409
External References
Database Identifier Description
NBRP SPAC4F10.16c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC4F10.16c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC4F10.16c BioGRID Interaction Datasets
Expression Viewer SPAC4F10.16c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC4F10.16c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC4F10.16c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC4F10.16c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC4F10.16c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC4F10.16c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC4F10.16c Cell Cycle Data
GEO SPAC4F10.16c GEO profiles
PInt SPAC4F10.16c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC4F10.16c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC4F10.16c Fission yeast phenotypic data & analysis
IntEnz3.6.3.1Integrated relational Enzyme database
Rhea3.6.3.1Annotated reactions database
SPD / RIKEN30/30F11Orfeome Localization Data
UniProtKB/SwissProtO36028Putative phospholipid-transporting ATPase C4F10.16c
ModBaseO36028Database of comparative protein structure models
STRINGO36028Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594759P-type ATPase (predicted)
RefSeq PeptideNP_594760conserved fungal protein
RefSeq mRNANM_001020186972h- P-type ATPase (predicted) (SPAC4F10.16c), mRNA
RefSeq mRNANM_001020187972h- conserved fungal protein (SPAC4F10.17), mRNA
European Nucleotide ArchiveCAB11719.1ENA Protein Mapping
UniParcUPI000006AA59UniProt Archive

Literature for SPAC4F10.16c

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014