rng2 (SPAC4F8.13c)

Gene Standard Namerng2 ChromosomeI
Systematic IDSPAC4F8.13c Gene Start2645380
Synonyms Gene End2650427
ProductIQGAP Gene Length5048
Feature Typeprotein coding CDS Start2645517
Name Description CDS End2649986
Characterisation Statuspublished CDS Length4470

Ensembl Gene Location
  1. itr1
  2. hcs1
  3. SPNCRNA.843
  4. rng2
  5. spp42
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000033abnormal contractile ring localizationMicroscopyrng2-M1 (L1092P,H1329L,K1366E)amino_acid_mutationPMID:21376595
FYPO:0000231abnormal contractile ring myosin filament organizationno_name (unrecorded)unrecordedPMID:19713940
FYPO:0000339mislocalized septumMicroscopyrng2-D5 (unknown)unknownPMID:21376595
Microscopyrng2-M1 (L1092P,H1329L,K1366E)amino_acid_mutationPMID:21376595
FYPO:0000729delayed actomyosin contractile ring assemblyMicroscopyrng2-M1 (L1092P,H1329L,K1366E)amino_acid_mutationPMID:21376595
FYPO:0000731abnormal protein localization to medial cortex during vegetative growthMicroscopyrng2-M1 (L1092P,H1329L,K1366E)amino_acid_mutationPMID:21376595
has_input rlc1
has_input cdc15Microscopyrng2-M1 (L1092P,H1329L,K1366E)amino_acid_mutationPMID:21376595
has_input cdc4Microscopyrng2-M1 (L1092P,H1329L,K1366E)amino_acid_mutationPMID:21376595
FYPO:0000733long mitotic spindleMicroscopyrng2-M1 (L1092P,H1329L,K1366E)amino_acid_mutationPMID:21376595
FYPO:0001008contractile ring absentrng2delta (deletion)deletionPMID:19713940
FYPO:0001489inviable vegetative cellMicroscopyrng2delta (deletion)deletionPMID:20473289
no_name (unrecorded)unrecordedPMID:9635188
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0003779actin bindingIDAPMID:9635188
GO:0005099Ras GTPase activator activityISMIPR000593GO_REF:0000001
GO:0005516calmodulin bindingIPIUniProtKB:P05933PMID:9635188
IDAPMID:9635188
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0000915cytokinesis, actomyosin contractile ring assemblyIMPPMID:21376595
IMPPMID:8834798
GO:0032187actomyosin contractile ring localizationIMPPMID:21376595
GO:0033205cell cycle cytokinesis
requiredIMPPMID:9635188
GO:0051017actin filament bundle assemblyIDAPMID:19713940
GO:0051056regulation of small GTPase mediated signal transductionIEAIPR001936GO_REF:0000002
GO:0071519actin filament bundle assembly involved in cytokinetic actomyosin contractile ring assemblyIDAPMID:19713940
GO:0071520actin filament bundle convergence involved in cytokinetic actomyosin contractile ring assemblyIDAPMID:19713940
GO:0071574protein localization to medial cortex
has_input cdc4IMPPMID:21376595
has_input cdc15IMPPMID:21376595
has_input rlc1IMPPMID:21376595
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0005635nuclear envelopeIEASL-0178GO_REF:0000039
GO:0005737cytoplasmIDAPMID:16823372
GO:0005816spindle pole bodyIDAPMID:9635188
IDAPMID:9635188
GO:0005826actomyosin contractile ringIDAPMID:9635188
GO:0030428cell septumIDAPMID:10759889
GO:0071341medial cortical node
exists_during mitosis, localization_dependent_on cdc4IDAPMID:21376595
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
126453802650427

References

Region Start End Reference
three_prime_UTR26499872650427PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF00612 IPR000048 Pfam IQ motif, EF-hand binding site 659 675 3
PF00612 IPR000048 Pfam IQ motif, EF-hand binding site 362 380 3
PF00307 IPR001715 Pfam Calponin homology domain 45 145 6
PF03836 IPR000593 Pfam RasGAP protein, C-terminal 1274 1414 2
PF00616 IPR001936 Pfam Ras GTPase-activating protein 875 1076 2
SM00015 IPR000048 SMART IQ motif, EF-hand binding site 447 469 5
SM00015 IPR000048 SMART IQ motif, EF-hand binding site 564 586 5
SM00015 IPR000048 SMART IQ motif, EF-hand binding site 417 439 5
SM00015 IPR000048 SMART IQ motif, EF-hand binding site 654 676 5
SM00015 IPR000048 SMART IQ motif, EF-hand binding site 358 380 5
SM00015 IPR000048 SMART IQ motif, EF-hand binding site 594 616 5
SM00015 IPR000048 SMART IQ motif, EF-hand binding site 388 410 5
SM00015 IPR000048 SMART IQ motif, EF-hand binding site 534 556 5
SM00323 IPR001936 SMART Ras GTPase-activating protein 840 1187 2
SM00033 IPR001715 SMART Calponin homology domain 43 143 5
PS50018 IPR001936 Prosite Profiles Ras GTPase-activating protein 867 1077 2
PS50096 IPR000048 Prosite Profiles IQ motif, EF-hand binding site 535 564 5
PS50096 IPR000048 Prosite Profiles IQ motif, EF-hand binding site 359 388 5
PS50096 IPR000048 Prosite Profiles IQ motif, EF-hand binding site 658 684 5
PS50096 IPR000048 Prosite Profiles IQ motif, EF-hand binding site 418 446 5
PS50096 IPR000048 Prosite Profiles IQ motif, EF-hand binding site 389 418 5
PS50096 IPR000048 Prosite Profiles IQ motif, EF-hand binding site 565 594 5
PS50021 IPR001715 Prosite Profiles Calponin homology domain 41 147 5
G3DSA:1.10.506.10 IPR001936 Gene3D 873 1103 2
G3DSA:1.10.418.10 IPR001715 Gene3D 34 171 6
SSF48350 IPR008936 SuperFamily 816 946 12
SSF48350 IPR008936 SuperFamily 973 1176 12
SSF143885 SuperFamily 1392 1489 2
SSF47576 IPR001715 SuperFamily 33 188 6
Coil ncoils Rabaptin coiled-coil domain 739 760 974
PTHR14149:SF4 hmmpanther 1 1489 1
PTHR14149 hmmpanther 1 1489 3

View domain organization at Pfam

Protein Modifications (PSI-MOD)

Term IDTerm NameEvidenceResidueReference
MOD:00696phosphorylated residuePMID:19547744

Manually Curated Family or Sub-families

Term IDTerm NameReferenceAll Genes
PBO:0001026IQ domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001026
PBO:0001435calponin homology (CH) domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001435

Protein Properties

Ave. residue weight 115.30 Da
Charge 49.00
Isoelectric point 9.66
Molecular weight 171.68 kDa
Number of residues 1489
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127		population_widePMID:23101633
experimental evidencevegetative growth of a single-celled organismPECO:0000014,
PECO:0000005		population_wide545.89PMID:23101633
experimental evidencevegetative growth of a single-celled organismPECO:0000201,
PECO:0000005		single_cell2700PMID:16224022
RNA levelexperimental evidencevegetative growth of a single-celled organismPECO:0000014,
PECO:0000005		population_wide2.5PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide1.2PMID:23101633
Species Distribution
DescriptionQualifierReference
conserved in fungi
conserved in eukaryotes
conserved in metazoa
conserved in vertebrates
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Synthetic Growth DefectPMID:15265986
ain1alpha-actinin Synthetic Growth DefectPMID:11294907
mob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Phenotypic SuppressionPMID:10769201
acp1F-actin capping protein alpha subunit Synthetic Growth DefectPMID:16866873
cdc4myosin II light chain Synthetic LethalityPMID:9635188
pxl1paxillin-like protein Pxl1 Synthetic LethalityPMID:18256290
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic LethalityPMID:20603077
Synthetic LethalityPMID:19139265
imp2contractile ring protein Imp2 Synthetic LethalityPMID:19139265
acp2F-actin capping protein beta subunit (predicted) Synthetic Growth DefectPMID:15743909
Synthetic Growth DefectPMID:16866873
Phenotypic Enhancement
myo2myosin II heavy chain Synthetic LethalityPMID:9635188
fim1fimbrin Synthetic Growth DefectPMID:11694585
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
pdx1pyruvate dehydrogenase protein x component, Pdx1 (predicted) Affinity Capture-MSPMID:20603077
cdc22ribonucleoside reductase large subunit Cdc22 Affinity Capture-MSPMID:20603077
mid1medial ring protein Mid1 Affinity Capture-WesternPMID:21422229
Affinity Capture-WesternPMID:22427686
Affinity Capture-WesternPMID:21376595
Reconstituted ComplexPMID:21376600
tom70mitochondrial TOM complex subunit Tom70 (predicted) Affinity Capture-MSPMID:20603077
ala1mitochondrial and cytoplasmic alanine-tRNA ligase Ala1 (predicted) Affinity Capture-MSPMID:20603077
rrs1ribosome biogenesis protein Rrs1 (predicted) Affinity Capture-MSPMID:20603077
myo51myosin type V Affinity Capture-RNAPMID:19805578
dfr1dihydrofolate reductase Dfr1 Affinity Capture-MSPMID:20603077
arf1ADP-ribosylation factor, Arf family Arf1 Affinity Capture-MSPMID:20603077
nrs1cytoplasmic asparagine-tRNA ligase Nrs1 (predicted) Affinity Capture-MSPMID:20603077
myo1myosin type I Affinity Capture-RNAPMID:19805578
rad2414-3-3 protein Rad24 Affinity Capture-MSPMID:20603077
rps260240S ribosomal protein S26 (predicted) Affinity Capture-MSPMID:20603077
cam1calmodulin Cam1 Affinity Capture-WesternPMID:9635188
sar1ADP-ribosylation factor Sar1 Affinity Capture-MSPMID:20603077
nog1GTP binding protein Nog1 (predicted) Affinity Capture-MSPMID:20603077
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Affinity Capture-MSPMID:20603077
Affinity Capture-Western
SPAC6F6.03cribosome export GTPase (predicted) Affinity Capture-MSPMID:20603077
SPAC18G6.05ctranslation elongation regulator Gcn1 (predicted) Affinity Capture-MSPMID:20603077
myo52myosin type V Affinity Capture-RNAPMID:19805578
myo2myosin II heavy chain Affinity Capture-WesternPMID:21422229
Affinity Capture-RNAPMID:19805578
SPAC1751.03translation initiation factor eIF3m Affinity Capture-MSPMID:20603077
SPBC691.04mitochondrial ATP-dependent RNA helicase Mss116 (predicted) Affinity Capture-MSPMID:20603077
hhf1histone H4 h4.1 Affinity Capture-MSPMID:20603077
cdc4myosin II light chain Two-hybridPMID:21422229
Affinity Capture-MSPMID:20603077
SPBC1711.05nucleocytoplasmic transport chaperone Srp40 (predicted) Affinity Capture-MSPMID:20603077
elf1AAA family ATPase Elf1 Affinity Capture-MSPMID:20603077
myp2myosin II heavy chain Myo3 Affinity Capture-RNAPMID:19805578
grn1GTPase Grn1 Affinity Capture-MSPMID:20603077
fim1fimbrin Reconstituted ComplexPMID:19713940
rpt119S proteasome regulatory subunit Rpt1 (predicted) Affinity Capture-MSPMID:20603077
grs1mitochondrial and cytoplasmic glycine-tRNA ligase Grs1 (predicted) Affinity Capture-MSPMID:20603077
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External References
Database Identifier Description
NBRP SPAC4F8.13c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC4F8.13c Retrieval of eukaryotic orthologs
BioGrid SPAC4F8.13c BioGRID Interaction Datasets
Bähler Lab SPAC4F8.13c Cell Cycle Expression Profile
Bähler Lab SPAC4F8.13c Meiosis/Sporulation Expression Profies
Bähler Lab SPAC4F8.13c Pheromone response/mating expression profiles
Bähler Lab SPAC4F8.13c Environmental stress expression profiles
Bähler Lab SPAC4F8.13c Bähler Lab Transcriptome Viewer
Cyclebase SPAC4F8.13c Cell Cycle Data
PInt SPAC4F8.13c Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPAC4F8.13c Entrez Gene
SPD / RIKEN30/30H04Orfeome Localization Data
WikiGene2543129IQ motif containing GTPase activating protein
Uniprot_genenameRNG2
EntrezGene2543129IQ motif containing GTPase activating protein
UniProtKB/SwissProtO14188Ras GTPase-activating-like protein rng2
ModBaseO14188Database of comparative protein structure models
Pfam Protein DomainsO14188Pfam Domain Arrangement
RefSeq PeptideNP_593860IQ motif containing GTPase activating protein
RefSeq mRNANM_001019289972h- IQ motif containing GTPase activating protein (rng2), mRNA
European Nucleotide ArchiveBAA87083ENA Protein Mapping
European Nucleotide ArchiveCAB11059ENA Protein Mapping
SPD / RIKEN30/30H04Orfeome Localization Data

Literature for rng2
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Search: UK PMC or PubMed

Release Version: PomBase:18.34 - 04 Apr 2013