rng2 (SPAC4F8.13c)


Gene Standard Namerng2 Characterisation Statuspublished
Systematic IDSPAC4F8.13c Feature Typeprotein coding
Synonyms Name Description
ProductIQGAP Product Size1489aa, 171.68 kDa
Genomic Location Chromosome I, 2645380-2650427 (5048nt); CDS:2645517-2649986 (4470nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005099Ras GTPase activator activityIEAIPR000593GO_REF:000000226
GO:0003779actin bindingIDAPMID:963518838
GO:0005516calmodulin bindingIDAPMID:963518810
IPIcam1PMID:9635188
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0051017actin filament bundle assemblyIDAPMID:1971394012
GO:0071519actin filament bundle assembly involved in cytokinetic actomyosin contractile ring assemblyIDAPMID:197139404
GO:0071520actin filament bundle convergence involved in cytokinetic actomyosin contractile ring assemblyIDAPMID:197139402
GO:1903479actin filament organization involved in mitotic actomyosin contractile ring assemblyIMPPMID:88347983
GO:1903475mitotic actomyosin contractile ring assemblyIMPPMID:2137659527
IMPPMID:8834798
GO:1902405mitotic actomyosin contractile ring localizationIMPPMID:213765957
GO:0000281mitotic cytokinesis132
requiredIMPPMID:9635188
GO:0071574protein localization to medial cortex6
localizes rlc1IMPPMID:21376595
localizes cdc15IMPPMID:21376595
localizes cdc4IMPPMID:21376595
GO:0051056regulation of small GTPase mediated signal transductionIEAIPR001936GO_REF:000000287
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005826actomyosin contractile ringIDAPMID:963518830
GO:0005737cytoplasmIDAPMID:168233724205
GO:0071341medial cortical node10
during mitotic M phaseIDAPMID:21376595
GO:0044732mitotic spindle pole bodyIDAPMID:9635188216
GO:0005634nucleusIEAUniProtKB-KW:KW-0539GO_REF:00000372740
GO:0005816spindle pole bodyIDAPMID:9635188225
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopynot recorded (unrecorded)PMID:96351881338
Microscopyrng2ΔNullPMID:20473289
Microscopyrng2ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002425abnormal actin cable organization during mitosisMicroscopyrng2-346Not specifiedPECO:0000004PMID:88347982
FYPO:0000033abnormal contractile ring localizationMicroscopyrng2-M1 (L1092P, H1329L, K1366E)PMID:2137659511
FYPO:0000231abnormal contractile ring myosin filament organizationnot recorded (unrecorded)PECO:0000004PMID:197139402
FYPO:0000059abnormal mitotic cell cycleMicroscopyrng2ΔNullPECO:0000005, PECO:0000137PMID:23697806686
FYPO:0000731abnormal protein localization to medial cortex during vegetative growth17
affecting cdc4Microscopyrng2-M1 (L1092P, H1329L, K1366E)PMID:21376595
affecting cdc15Microscopyrng2-M1 (L1092P, H1329L, K1366E)PMID:21376595
affecting rlc1Microscopyrng2-M1 (L1092P, H1329L, K1366E)PMID:21376595
FYPO:0000117abnormal septationMicroscopyrng2-346Not specifiedPECO:0000004PMID:8834798107
FYPO:0001008contractile ring absentMicroscopyrng2ΔNullPMID:1971394060
FYPO:0000729delayed actomyosin contractile ring assemblyMicroscopyrng2-M1 (L1092P, H1329L, K1366E)PMID:213765951
FYPO:0000252increased spontaneous diploidizationOtherrng2-346Not specifiedPMID:883479823
FYPO:0000314inviable after spore germination with elongated germ tube163
penetrance FYPO_EXT:0000001Microscopyrng2ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0002025inviable elongated multinucleate vegetative cell with abnormal septum morphologyMicroscopyrng2-346Not specifiedPECO:0000004PMID:88347982
FYPO:0000733long mitotic spindleMicroscopyrng2-M1 (L1092P, H1329L, K1366E)PMID:213765956
FYPO:0000339mislocalized septumMicroscopyrng2-D5PMID:2137659530
Microscopyrng2-M1 (L1092P, H1329L, K1366E)PMID:21376595
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in cdc4 myosin II light chain PMID:21376595
GO localized by cdc4 myosin II light chain PMID:21376595
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
126453802650427

UTRs

Region Coordinates Reference
five_prime_UTR2645380..2645516PMID:21511999
three_prime_UTR2649987..2650427PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00307 Pfam IPR001715 Calponin homology domain 45 145 6
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 659 675 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 362 380 3
PF00616 Pfam IPR001936 Ras GTPase-activating protein 875 1076 2
PF03836 Pfam IPR000593 RasGAP protein, C-terminal 1274 1414 2
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 388 410 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 417 439 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 534 556 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 564 586 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 654 676 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 447 469 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 594 616 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 358 380 3
SM00033 SMART IPR001715 Calponin homology domain 43 143 5
SM00323 SMART IPR001936 Ras GTPase-activating protein 840 1187 2
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 418 446 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 565 594 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 535 564 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 359 388 5
PS50018 Prosite Profiles IPR001936 Ras GTPase-activating protein 867 1077 2
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 389 418 5
PS50021 Prosite Profiles IPR001715 Calponin homology domain 41 147 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 658 684 5
PTHR14149 HMMPANTHER 30 1489 3
PTHR14149:SF4 HMMPANTHER 30 1489 1
1.10.418.10 Gene3D IPR001715 Calponin homology domain 34 171 6
1.10.506.10 Gene3D IPR001936 Ras GTPase-activating protein 873 1103 2
1wdcA00 Gene3D IPR027401 Myosin-like IQ motif-containing domain 389 423 3
SSF47576 SuperFamily IPR001715 Calponin homology domain 33 188 6
0050767 SuperFamily 1392 1489 2
SSF48350 SuperFamily IPR008936 Rho GTPase activation protein 973 1176 12
SSF48350 SuperFamily IPR008936 Rho GTPase activation protein 816 946 12
Coil ncoils Rabaptin coiled-coil domain 739 760 968

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001026IQ domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001026
PBO:0001435calponin homology (CH) domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001435

Protein Properties

Ave. residue weight 115.30 Da
Charge 49.00
Isoelectric point 9.66
Molecular weight 171.68 kDa
Number of residues 1489
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS23PMID:217125471670
present during mitotic M phase
MOD:00047O-phospho-L-threonineexperimental evidenceT25PMID:24763107692
present during mitotic M phaseexperimental evidenceT25PMID:21712547
MOD:00696phosphorylated residueNASPMID:195477441922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1664during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1508during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1205during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1337during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1435during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
545.89during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
2700during GO:0072690PECO:0000005,
PECO:0000012
single_cellexperimental evidencePMID:16224022
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.5during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Synthetic Growth DefectPMID:15265986
acp1F-actin capping protein alpha subunit Synthetic Growth DefectPMID:16866873
myo2myosin II heavy chain Synthetic LethalityPMID:9635188
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic LethalityPMID:19139265
Synthetic LethalityPMID:20603077
mob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Phenotypic SuppressionPMID:10769201
acp2F-actin capping protein beta subunit Acp2 Phenotypic EnhancementPMID:16866873
Synthetic Growth Defect
Synthetic Growth DefectPMID:15743909
nod1medial cortical node Gef2-related protein protein Nod1 Phenotypic EnhancementPMID:23966468
gef2RhoGEF Gef2 Phenotypic EnhancementPMID:23966468
blt1ubiquitin domain-like protein Blt1 Synthetic Growth DefectPMID:24790095
for3formin For3 Synthetic LethalityPMID:24127216
imp2contractile ring protein Imp2 Synthetic LethalityPMID:19139265
cdc12formin Cdc12 Synthetic LethalityPMID:24127216
fim1fimbrin Synthetic Growth DefectPMID:11694585
ain1alpha-actinin Synthetic Growth DefectPMID:11294907
pxl1paxillin-like protein Pxl1 Synthetic LethalityPMID:18256290
cdc4myosin II light chain Synthetic LethalityPMID:9635188
Synthetic RescuePMID:23615450
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
myo2myosin II heavy chain Affinity Capture-RNAPMID:19805578
Affinity Capture-WesternPMID:21422229
SPBC691.04mitochondrial ATP-dependent RNA helicase Mss116 (predicted) Affinity Capture-MSPMID:20603077
rad2414-3-3 protein Rad24 Affinity Capture-MSPMID:20603077
myo1myosin type I Affinity Capture-RNAPMID:19805578
SPAC18G6.05ctranslation elongation regulator Gcn1 (predicted) Affinity Capture-MSPMID:20603077
dfr1dihydrofolate reductase/ serine hydrolase family fusion protein Dfr1 Affinity Capture-MSPMID:20603077
cdc22ribonucleoside reductase large subunit Cdc22 Affinity Capture-MSPMID:20603077
mid1medial ring protein Mid1 Affinity Capture-WesternPMID:21422229
Affinity Capture-WesternPMID:22427686
Affinity Capture-WesternPMID:21376595
Reconstituted ComplexPMID:21376600
cdc4myosin II light chain Two-hybridPMID:21422229
Affinity Capture-MSPMID:20603077
myo52myosin type V Affinity Capture-RNAPMID:19805578
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
rps260240S ribosomal protein S26 (predicted) Affinity Capture-MSPMID:20603077
grn1GTPase Grn1 Affinity Capture-MSPMID:20603077
myo51myosin type V Affinity Capture-RNAPMID:19805578
SPAC1751.03translation initiation factor eIF3m Affinity Capture-MSPMID:20603077
rrs1ribosome biogenesis protein Rrs1 (predicted) Affinity Capture-MSPMID:20603077
fim1fimbrin Reconstituted ComplexPMID:19713940
grs1mitochondrial and cytoplasmic glycine-tRNA ligase Grs1 Affinity Capture-MSPMID:20603077
elf1AAA family ATPase Elf1 Affinity Capture-MSPMID:20603077
arf1ADP-ribosylation factor, Arf family Arf1 Affinity Capture-MSPMID:20603077
tom70mitochondrial TOM complex subunit Tom70 (predicted) Affinity Capture-MSPMID:20603077
cam1calmodulin Cam1 Affinity Capture-WesternPMID:9635188
SPAC6F6.03cribosome export GTPase (predicted) Affinity Capture-MSPMID:20603077
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Affinity Capture-WesternPMID:20603077
Affinity Capture-MS
rpt119S proteasome regulatory subunit Rpt1 (predicted) Affinity Capture-MSPMID:20603077
hhf1histone H4 h4.1 Affinity Capture-MSPMID:20603077
sar1ADP-ribosylation factor Sar1 Affinity Capture-MSPMID:20603077
ala1mitochondrial and cytoplasmic alanine-tRNA ligase Ala1 (predicted) Affinity Capture-MSPMID:20603077
myp2myosin II heavy chain Myo3 Affinity Capture-RNAPMID:19805578
pdx1pyruvate dehydrogenase protein x component, Pdx1 (predicted) Affinity Capture-MSPMID:20603077
nrs1cytoplasmic asparagine-tRNA ligase Nrs1 (predicted) Affinity Capture-MSPMID:20603077
nog1GTP binding protein Nog1 (predicted) Affinity Capture-MSPMID:20603077
SPBC1711.05nucleocytoplasmic transport chaperone Srp40 (predicted) Affinity Capture-MSPMID:20603077
External References
Database Identifier Description
NBRP SPAC4F8.13c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC4F8.13c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC4F8.13c BioGRID Interaction Datasets
Expression Viewer SPAC4F8.13c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC4F8.13c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC4F8.13c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC4F8.13c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC4F8.13c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC4F8.13c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC4F8.13c Cell Cycle Data
GEO SPAC4F8.13c GEO profiles
PInt SPAC4F8.13c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC4F8.13c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC4F8.13c Fission yeast phenotypic data & analysis
SPD / RIKEN30/30H04Orfeome Localization Data
UniProtKB/SwissProtO14188Ras GTPase-activating-like protein rng2
ModBaseO14188Database of comparative protein structure models
STRINGO14188Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593860IQ motif containing GTPase activating protein
RefSeq mRNANM_001019289972h- IQ motif containing GTPase activating protein (rng2), mRNA
ePDB1P2XThe European PDB
PDB1P2XPDB
PDBsum1P2XPDBsum
ePDB1P5SThe European PDB
PDB1P5SPDB
PDBsum1P5SPDBsum
European Nucleotide ArchiveBAA87083.1ENA Protein Mapping
European Nucleotide ArchiveCAB11059.1ENA Protein Mapping
UniParcUPI000006CC6FUniProt Archive

Literature for rng2

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014