rng2 (SPAC4F8.13c)


Gene Standard Namerng2 Characterisation Statuspublished
Systematic IDSPAC4F8.13c Feature Typeprotein coding
Synonyms Name Description
ProductIQGAP Product Size1489aa, 171.68 kDa
Genomic Location Chromosome I, 2645380-2650427 (5048nt); CDS:2645517-2649986 (4470nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
actin binding37
Annotation ExtensionEvidenceWith/FromReference
calmodulin binding13
Annotation ExtensionEvidenceWith/FromReference
GTPase activator activity43
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
actin filament bundle convergence involved in mitotic contractile ring assembly2
Annotation ExtensionEvidenceWith/FromReference
mitotic actomyosin contractile ring assembly27
Annotation ExtensionEvidenceWith/FromReference
mitotic actomyosin contractile ring assembly actin filament organization7
Annotation ExtensionEvidenceWith/FromReference
mitotic actomyosin contractile ring localization8
Annotation ExtensionEvidenceWith/FromReference
mitotic contractile ring actin filament bundle assembly3
Annotation ExtensionEvidenceWith/FromReference
mitotic cytokinesis104
Annotation ExtensionEvidenceWith/FromReference
protein localization to medial cortex6
Annotation ExtensionEvidenceWith/FromReference
regulation of GTPase activity46
Annotation ExtensionEvidenceWith/FromReference
signal transduction291
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actomyosin contractile ring39
Annotation ExtensionEvidenceWith/FromReference
cytoplasm4213
Annotation ExtensionEvidenceWith/FromReference
medial cortical node11
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle pole body220
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationrng2+ (wild type)1455
rng2Δ

Cell Phenotype

Term NameGenotypesCount
abnormal actin cable organization during mitosisrng2-3462
abnormal actomyosin contractile ring assemblyrng2-D516
abnormal actomyosin contractile ring localizationrng2-M1 (L1092P, H1329L, K1366E)13
abnormal actomyosin contractile ring myosin filament organization2
abnormal mitotic cell cyclerng2Δ894
abnormal protein localization to medial cortex during vegetative growth31
affects localization of cdc15rng2-M1 (L1092P, H1329L, K1366E)
affects localization of rlc1rng2-M1 (L1092P, H1329L, K1366E)
affects localization of cdc4rng2-M1 (L1092P, H1329L, K1366E)
abnormal septationrng2-346296
actomyosin contractile ring absentrng2Δ9
delayed onset of actomyosin contractile ring assemblyrng2-M1 (L1092P, H1329L, K1366E)2
inviable after spore germination with elongated germ tuberng2Δ164
inviable elongated multinucleate vegetative cell with abnormal septum morphologyrng2-3462
long mitotic spindlerng2-M1 (L1092P, H1329L, K1366E)24
mislocalized septum during vegetative growthrng2-M1 (L1092P, H1329L, K1366E)45
rng2-D5

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
inviable vegetative cell populationrng2-D5, pxl1Δ
cdc12-P31A (P31A), rng2-D5

Cell Phenotype

Term NameGenotypes
inviable branched, elongated, multiseptate vegetative cellrng2-D5, pxl1Δ
Target Of
OntologyRelationshipGeneProduct
FYPO localization affected by mutation in cdc4 myosin II light chain
GO localized by cdc4 myosin II light chain
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2645380..2650427
mRNA2645380..2650427
5' UTR2645380..2645516PMID:21511999
CDS2645517..2649986
3' UTR2649987..2650427PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 361 380 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 659 675 3
PF00616 Pfam IPR001936 Ras GTPase-activating protein 875 1076 2
PF03836 Pfam IPR000593 RasGAP protein, C-terminal 1274 1411 2
PF00307 Pfam IPR001715 Calponin homology domain 44 144 5
SM00323 SMART IPR001936 Ras GTPase-activating protein 840 1187 2
SM00033 SMART IPR001715 Calponin homology domain 43 143 5
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 417 439 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 447 469 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 358 380 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 388 410 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 594 616 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 654 676 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 534 556 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 564 586 3
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 359 388 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 389 418 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 418 446 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 565 594 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 535 564 5
PS50018 Prosite Profiles IPR001936 Ras GTPase-activating protein 867 1077 2
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 658 684 5
PS50021 Prosite Profiles IPR001715 Calponin homology domain 41 147 5
PTHR14149 HMMPANTHER 20 1488 3
PTHR14149:SF14 HMMPANTHER 20 1488 1
1.10.506.10 Gene3D IPR001936 Ras GTPase-activating protein 873 1103 2
1.10.418.10 Gene3D IPR001715 Calponin homology domain 34 171 6
1wdcA00 Gene3D IPR027401 Myosin-like IQ motif-containing domain 389 423 3
SSF48350 SuperFamily IPR008936 Rho GTPase activation protein 816 946 12
SSF48350 SuperFamily IPR008936 Rho GTPase activation protein 973 1176 12
0050767 SuperFamily Tenascin-R 1392 1489 2
SSF47576 SuperFamily IPR001715 Calponin homology domain 33 188 6
Coil ncoils Predicted coiled-coil protein (DUF2205) 739 759 1055

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0001026IQ domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001026
PBO:0001435calponin homology (CH) domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001435

Protein Properties

Ave. residue weight 115.30 Da
Charge 49.00
Codon Adaptation Index 0.38
Isoelectric point 9.66
Molecular weight 171.68 kDa
Number of residues 1489
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during mitotic M phaseS23
S23, S207, S260
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S23 PMID:21712547
IDA S207 PMID:25720772
IDA S23 PMID:25720772
IDA S260 PMID:25720772
O-phospho-L-threonine 1085
present during mitotic M phaseT25
T20, T25, T258
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T25 PMID:21712547
IDA T258 PMID:25720772
IDA T20 PMID:25720772
IDA T25 PMID:25720772
experimental evidence T25 PMID:24763107
phosphorylated residue 2511
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
O-phosphorylated residueS199,S202,S203, S199,S202,S207, S199,S202,T205, S199,S203,S207, S199,S203,T209, S199,T205,S207, S202,S203, S260,S264, T258,S264, T262,S264 2457
Annotation ExtensionEvidenceResidueReference
IDA S199,S203,T209 PMID:25720772
IDA S199,S203,S207 PMID:25720772
IDA S199,T205,S207 PMID:25720772
IDA S260,S264 PMID:25720772
IDA T258,S264 PMID:25720772
IDA S199,S202,T205 PMID:25720772
IDA S199,S202,S203 PMID:25720772
IDA S202,S203 PMID:25720772
IDA T262,S264 PMID:25720772
IDA S199,S202,S207 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for rng2 (SPAC4F8.13c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1664during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1508during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1205during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1337during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2700during GO:0072690PECO:0000012,
PECO:0000005
single cellexperimental evidencePMID:16224022
545.89during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1435during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.5during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC4F8.13c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bycam1calmodulin Cam1 Affinity Capture-WesternPMID:9635188
affinity captured bycdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Affinity Capture-WesternPMID:20603077
affinity captured byclp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
affinity captured bymyo51myosin type V Affinity Capture-RNAPMID:19805578
affinity captured bymyo1myosin type I Affinity Capture-RNAPMID:19805578
affinity captured bymyo2myosin II heavy chain Affinity Capture-RNAPMID:19805578
affinity captured bymyp2myosin II heavy chain Myo3 Affinity Capture-RNAPMID:19805578
affinity captured bymyo52myosin type V Affinity Capture-RNAPMID:19805578
affinity captureself1AAA family ATPase Elf1 Affinity Capture-MSPMID:20603077
affinity capturesnog2ribosome export GTPase Nog2 (predicted) Affinity Capture-MSPMID:20603077
affinity capturescdc22ribonucleoside reductase large subunit Cdc22 Affinity Capture-MSPMID:20603077
affinity capturesmid1medial ring protein Mid1 Affinity Capture-WesternPMID:22427686
affinity capturestif313translation initiation factor eIF3m Affinity Capture-MSPMID:20603077
affinity capturesdfr1dihydrofolate reductase/ serine hydrolase family fusion protein Dfr1 Affinity Capture-MSPMID:20603077
affinity capturescdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Affinity Capture-MSPMID:20603077
affinity capturesala1mitochondrial and cytoplasmic alanine-tRNA ligase Ala1 (predicted) Affinity Capture-MSPMID:20603077
affinity capturesrrs1ribosome biogenesis protein Rrs1 Affinity Capture-MSPMID:20603077
affinity capturesgcn1translation elongation regulator Gcn1 (predicted) Affinity Capture-MSPMID:20603077
affinity capturesmyo2myosin II heavy chain Affinity Capture-WesternPMID:21422229
affinity capturesnog1GTP binding protein Nog1 (predicted) Affinity Capture-MSPMID:20603077
affinity captureshhf1histone H4 h4.1 Affinity Capture-MSPMID:20603077
affinity capturesarf1ADP-ribosylation factor, Arf family Arf1 Affinity Capture-MSPMID:20603077
affinity capturesrad2414-3-3 protein Rad24 Affinity Capture-MSPMID:20603077
affinity capturesgrs1mitochondrial and cytoplasmic glycine-tRNA ligase Grs1 Affinity Capture-MSPMID:20603077
affinity capturesgrn1GTPase Grn1 Affinity Capture-MSPMID:20603077
affinity capturestom70mitochondrial TOM complex subunit Tom70 (predicted) Affinity Capture-MSPMID:20603077
affinity capturespdx1pyruvate dehydrogenase protein x component, Pdx1 (predicted) Affinity Capture-MSPMID:20603077
affinity capturesSPBC1711.05nucleocytoplasmic transport chaperone Srp40 (predicted) Affinity Capture-MSPMID:20603077
affinity capturescam1calmodulin Cam1 Affinity Capture-WesternPMID:9635188
affinity capturesrpt119S proteasome regulatory subunit Rpt1 (predicted) Affinity Capture-MSPMID:20603077
affinity capturescdc4myosin II light chain Affinity Capture-MSPMID:20603077
affinity capturesmss116mitochondrial ATP-dependent RNA helicase Mss116 (predicted) Affinity Capture-MSPMID:20603077
affinity capturesrps260240S ribosomal protein S26 (predicted) Affinity Capture-MSPMID:20603077
affinity capturessar1ADP-ribosylation factor Sar1 Affinity Capture-MSPMID:20603077
affinity capturesnrs1cytoplasmic asparagine-tRNA ligase Nrs1 (predicted) Affinity Capture-MSPMID:20603077
binds activation domain construct withcdc4myosin II light chain Two-hybridPMID:21422229
forms complex withmid1medial ring protein Mid1 Reconstituted ComplexPMID:21376600
forms complex withfim1fimbrin Reconstituted ComplexPMID:19713940
modified bydsk1SR protein-specific kinase Dsk1 Biochemical ActivityPMID:26167880
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC4F8.13c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withcdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic LethalityPMID:19139265
synthetic lethal withpxl1paxillin-like protein Pxl1 Synthetic LethalityPMID:18256290
synthetic lethal withcdc4myosin II light chain Synthetic LethalityPMID:9635188
synthetic lethal withrga7Rho-type GTPase activating protein Rga7 Synthetic LethalityPMID:25977474
synthetic lethal withmyo2myosin II heavy chain Synthetic LethalityPMID:9635188
synthetic lethal withcdc12formin Cdc12 Synthetic LethalityPMID:24127216
synthetic lethal withfor3formin For3 Synthetic LethalityPMID:24127216
synthetic lethal withimp2contractile ring protein Imp2 Synthetic LethalityPMID:19139265
synthetic growth defect withain1alpha-actinin Synthetic Growth DefectPMID:11294907
synthetic growth defect withblt1ubiquitin domain-like protein Blt1 Synthetic Growth DefectPMID:24790095
synthetic growth defect withacp2F-actin capping protein beta subunit Acp2 Synthetic Growth DefectPMID:15743909
synthetic growth defect withfim1fimbrin Synthetic Growth DefectPMID:11694585
synthetic growth defect withclp1Cdc14-related protein phosphatase Clp1/Flp1 Synthetic Growth DefectPMID:15265986
synthetic growth defect withacp1F-actin capping protein alpha subunit Synthetic Growth DefectPMID:16866873
synthetically rescuescdc4myosin II light chain Synthetic RescuePMID:23615450
enhances phenotype ofacp2F-actin capping protein beta subunit Acp2 Phenotypic EnhancementPMID:16866873
rescued bymob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Phenotypic SuppressionPMID:10769201
phenotype enhanced bynod1medial cortical node Gef2-related protein protein Nod1 Phenotypic EnhancementPMID:23966468
phenotype enhanced bygef2RhoGEF Gef2 Phenotypic EnhancementPMID:23966468
External References
Database Identifier Description
NBRP SPAC4F8.13c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC4F8.13c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC4F8.13c BioGRID Interaction Datasets
Expression Viewer SPAC4F8.13c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC4F8.13c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC4F8.13c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC4F8.13c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC4F8.13c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC4F8.13c Transcriptome Viewer (Bähler Lab)
GEO SPAC4F8.13c GEO profiles
PInt SPAC4F8.13c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC4F8.13c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC4F8.13c Fission yeast phenotypic data & analysis
Cyclebase SPAC4F8.13c.1 Cell Cycle Data
SPD / RIKEN30/30H04Orfeome Localization Data
UniProtKB/SwissProtO14188Ras GTPase-activating-like protein rng2
ModBaseO14188Database of comparative protein structure models
STRINGO14188Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593860IQ motif containing GTPase activating protein
RefSeq mRNANM_001019289972h- IQ motif containing GTPase activating protein (rng2), mRNA
ePDB1P2XThe European PDB
PDB1P2XPDB
PDBsum1P2XPDBsum
ePDB1P5SThe European PDB
PDB1P5SPDB
PDBsum1P5SPDBsum
European Nucleotide ArchiveBAA87083.1ENA Protein Mapping
European Nucleotide ArchiveCAB11059.1ENA Protein Mapping
UniParcUPI000006CC6FUniProt Archive

Literature for rng2

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016