arg11 (SPAC4G9.09c)


Gene Standard Namearg11 Characterisation Statuspublished
Systematic IDSPAC4G9.09c Feature Typeprotein coding
Synonyms Name Description
ProductN-acetyl-gamma-glutamyl-phosphate reductase/acetylglutamate kinase Product Size885aa, 97.70 kDa
Genomic Location Chromosome I, 2272911-2269908 (3004nt); CDS:2272676-2270019 (2658nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
acetylglutamate kinase activity1
Annotation ExtensionEvidenceWith/FromReference
ATP binding559
Annotation ExtensionEvidenceWith/FromReference
N-acetyl-gamma-glutamyl-phosphate reductase activity1
Annotation ExtensionEvidenceWith/FromReference
NAD binding23
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
arginine biosynthetic process12
Annotation ExtensionEvidenceWith/FromReference
ornithine biosynthetic process4
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
mitochondrial matrix153
Annotation ExtensionEvidenceWith/FromReference
mitochondrion752
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
abolished cell population growth on glycerol carbon sourcearg11Δ68
decreased vegetative cell population growtharg11Δ634
sensitive to hydrogen peroxidearg11Δ153
viable vegetative cell populationarg11Δ3831

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologyarg11Δ3099
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2272911..2269908
5' UTR2272911..2272677PMID:21511999
3' UTR2270018..2269908AU011644
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00696 Pfam IPR001048 Aspartate/glutamate/uridylate kinase 94 309 3
PF01118 Pfam IPR000534 Semialdehyde dehydrogenase, NAD-binding 572 693 2
PF04768 Pfam IPR006855 Vertebrate-like NAGS Gcn5-related N-acetyltransferase (GNAT) domain 327 490 2
SM00859 SMART IPR000534 Semialdehyde dehydrogenase, NAD-binding 572 694 2
PS01224 Prosite Patterns IPR023013 N-acetyl-gamma-glutamyl-phosphate reductase, active site 698 714 1
PS51731 Prosite Profiles IPR006855 Vertebrate-like NAGS Gcn5-related N-acetyltransferase (GNAT) domain 346 499 2
PTHR23342 HMMPANTHER 32 494 2
PTHR23342 HMMPANTHER 613 657 2
PTHR23342:SF0 HMMPANTHER 32 494 1
PTHR23342:SF0 HMMPANTHER 613 657 1
3.30.360.10 Gene3D Oxidoreductase, C-terminal 709 857 14
3.40.1160.10 Gene3D IPR001048 Aspartate/glutamate/uridylate kinase 91 343 3
3.40.50.720 Gene3D IPR016040 NAD(P)-binding domain 569 708 100
SSF51735 SuperFamily IPR016040 NAD(P)-binding domain 570 720 88
SSF53633 SuperFamily IPR001048 Aspartate/glutamate/uridylate kinase 67 344 3
SSF55347 SuperFamily 703 857 12
PIRSF036440 PIRSF IPR011241 NAGK-NAGSA, bifunctional 1 885 1
TIGR00761 tigrfam IPR004662 Acetylglutamate kinase 94 319 1
TIGR01850 tigrfam IPR000706 N-acetyl-gamma-glutamyl-phosphate reductase, type 1 572 883 1

View domain organization at Pfam

Term IDTerm NameReferenceCount
SO:0100011cleaved_peptide_region296

Protein Properties

Ave. residue weight 110.40 Da
Charge 30.00
Codon Adaptation Index 0.50
Isoelectric point 9.77
Molecular weight 97.70 kDa
Number of residues 885
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1675
present during mitotic M phaseS836
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S836 PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for arg11 (SPAC4G9.09c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
64389during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
70759during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
67388during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
63584during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
26527.97during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
67667during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
33200.01during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8.5during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
3.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
metabolic disorders143
urea cycle disorders7
N-Acetylglutamate Synthetase deficiency2
Genome Organisation
DescriptionQualifierReferenceCount
divergently orientated to functionally related gene10
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3090
conserved in fungi4604
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3422
conserved in vertebrates3397
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load gene that interact physically with SPAC4G9.09c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturestrx2mitochondrial thioredoxin Trx2 Affinity Capture-WesternPMID:18849471
Genetic Interactions

Source: BioGRID

Load gene that interact genetically with SPAC4G9.09c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
overexpression rescuestrx2mitochondrial thioredoxin Trx2 Dosage RescuePMID:18849471
negative genetic interaction withxap5xap-5-like protein Negative GeneticPMID:24957674
negative genetic interaction withrad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Negative GeneticPMID:22681890
negative genetic interaction withsre1sterol regulatory element binding protein, transcription factor Sre1 Negative GeneticPMID:21504829
negative genetic interaction withmlo3RNA binding protein Mlo3 Negative GeneticPMID:22681890
negative genetic interaction witheso1sister chromatid cohesion protein/DNA polymerase eta Eso1 fusion protein Negative GeneticPMID:23050226
negative genetic interaction withsrb11cyclin CycC, Srb mediator subunit Srb11 Negative GeneticPMID:22681890
negative genetic interaction withsfp1transcription factor Sfp1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmcs2TFIIH complex cyclin Mcs2 Negative GeneticPMID:22681890
negative genetic interaction withski3Ski complex TPR repeat subunit Ski3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withvps17retromer complex subunit Vps17 Negative GeneticPMID:22681890
negative genetic interaction withSPBC18H10.05WD repeat protein, human WDR44 family Negative GeneticPMID:22681890
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
negative genetic interaction withgim6prefoldin subunit 1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Negative GeneticPMID:22681890
negative genetic interaction withebs1EST1 family nonsense-mediated mRNA decay (NMD) pathway protein Ebs1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withvps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
negative genetic interaction withdsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:22681890
negative genetic interaction withtif38translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
negative genetic interaction withrad17RFC related checkpoint protein Rad17 Negative GeneticPMID:22681890
negative genetic interaction withprp38U4/U6 x U5 tri-snRNP complex subunit Prp38 Negative GeneticPMID:22681890
negative genetic interaction withbun107WD repeat protein, human WDR48 family Bun107 Negative GeneticPMID:22681890
negative genetic interaction withsre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
negative genetic interaction withrem1meiosis-specific cyclin Rem1 Negative GeneticPMID:22681890
negative genetic interaction withfar10SIP/FAR complex FHA domain subunit Far10/Csc1 Negative GeneticPMID:22681890
negative genetic interaction witharp8actin-like protein, Ino80 complex subunit Arp8 Negative GeneticPMID:22681890
negative genetic interaction withpof9F-box protein Pof9 Negative GeneticPMID:22681890
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
negative genetic interaction withhbs1elongation factor 1 alpha related protein Hbs1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
negative genetic interaction withpub3HECT-type ubiquitin-protein ligase E3 Pub3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpab2poly(A) binding protein Pab2 Negative GeneticPMID:22681890
negative genetic interaction withcdc37Hsp90 co-chaperone Cdc37 Negative GeneticPMID:22681890
negative genetic interaction withsaf4splicing associated factor Saf4 Negative GeneticPMID:22681890
negative genetic interaction withcdk11serine/threonine protein kinase cdk11 Negative GeneticPMID:22681890
negative genetic interaction withmed14mediator complex subunit Med14 Negative GeneticPMID:22681890
negative genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmpr1histidine-containing response regulator phosphotransferase Mpr1 Negative GeneticPMID:22681890
positive genetic interaction withfkbp39FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withsty1MAP kinase Sty1 Positive GeneticPMID:22681890
positive genetic interaction withppk8serine/threonine protein kinase Ppk8 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withxks1xylulose kinase Xks1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withsts5RNB-like protein Positive GeneticPMID:22681890
positive genetic interaction withpar1protein phosphatase PP2A regulatory subunit B-56 Par1 Positive GeneticPMID:22681890
positive genetic interaction withdad1DASH complex subunit Dad1 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC4G9.09c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC4G9.09c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC4G9.09c BioGRID Interaction Datasets
Expression Viewer SPAC4G9.09c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC4G9.09c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC4G9.09c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC4G9.09c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC4G9.09c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC4G9.09c Transcriptome Viewer (Bähler Lab)
GEO SPAC4G9.09c GEO profiles
PInt SPAC4G9.09c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC4G9.09c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC4G9.09c Fission yeast phenotypic data & analysis
Cyclebase SPAC4G9.09c.1 Cell Cycle Data
IntEnz1.2.1.38Integrated relational Enzyme database
Rhea1.2.1.38Annotated reactions database
KEGGMAP00220KEGG Pathway Database
SPD / RIKEN47/47A09Orfeome Localization Data
IntEnz2.7.2.8Integrated relational Enzyme database
Rhea2.7.2.8Annotated reactions database
UniProtKB/SwissProtP31318Protein arg11, mitochondrial N-acetyl-gamma-glutamyl-phosphate reductase Acetylglutamate kinase
ModBaseP31318Database of comparative protein structure models
STRINGP31318Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593691N-acetyl-gamma-glutamyl-phosphate reductase/acetylglutamate kinase
RefSeq mRNANM_001019123972h- N-acetyl-gamma-glutamyl-phosphate reductase/acetylglutamate kinase (arg11), mRNA
European Nucleotide ArchiveCU329670ENA EMBL mapping
European Nucleotide ArchiveX63576ENA EMBL mapping
European Nucleotide ArchiveCAA45132ENA Protein Mapping
European Nucleotide ArchiveCAA93559ENA Protein Mapping
MetaCycPWY-5154L-arginine biosynthesis III (via N-acetyl-L-citrulline)
MetaCycPWY-7400L-arginine biosynthesis IV (archaebacteria)
KEGG00220+1.2.1.38Arginine biosynthesis
KEGG00220+1.2.1.38+2.7.2.8Arginine biosynthesis
KEGG00220+2.3.1.1Arginine biosynthesis
KEGG00220+2.7.2.8Arginine biosynthesis
UniParcUPI0000125D42UniProt Archive
UniPathwayUPA00068Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3

Literature for arg11

Search: Europe PMC or PubMed

Release Version: PomBase:30_57 - 27 Jan 2016