arg11 (SPAC4G9.09c)


Gene Standard Namearg11 Characterisation Statuspublished
Systematic IDSPAC4G9.09c Feature Typeprotein coding
Synonyms Name Description
ProductN-acetyl-gamma-glutamyl-phosphate reductase/acetylglutamate kinase Product Size885aa, 97.70 kDa
Genomic Location Chromosome I, 2272911-2269908 (3004nt); CDS:2272676-2270019 (2658nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
acetylglutamate kinase activity1
Annotation ExtensionEvidenceWith/FromReference
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
N-acetyl-gamma-glutamyl-phosphate reductase activity1
Annotation ExtensionEvidenceWith/FromReference
NAD binding23
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
arginine biosynthetic process12
Annotation ExtensionEvidenceWith/FromReference
ornithine biosynthetic process4
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
mitochondrial matrix153
Annotation ExtensionEvidenceWith/FromReference
mitochondrion761
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
abolished cell population growth on glycerol carbon sourcearg11ΔNull67
decreased vegetative cell population growtharg11ΔNull571
sensitive to hydrogen peroxidearg11ΔNull139
viable vegetative cell populationarg11ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologyarg11ΔNull3094
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2272911..2269908
mRNA2272911..2269908
5' UTR2272911..2272677PMID:21511999
CDS2272676..2270019
3' UTR2270018..2269908AU011644
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00696 Pfam IPR001048 Aspartate/glutamate/uridylate kinase 94 311 3
PF01118 Pfam IPR000534 Semialdehyde dehydrogenase, NAD-binding 572 693 2
PF04768 Pfam IPR006855 Domain of unknown function DUF619 327 490 2
SM00859 SMART IPR000534 Semialdehyde dehydrogenase, NAD-binding 572 694 2
PS01224 Prosite Patterns IPR023013 N-acetyl-gamma-glutamyl-phosphate reductase, active site 698 714 1
PTHR23342:SF0 HMMPANTHER 58 867 1
PTHR23342 HMMPANTHER 58 867 2
3.40.1160.10 Gene3D IPR001048 Aspartate/glutamate/uridylate kinase 91 343 3
3.30.360.10 Gene3D 709 857 14
3.40.50.720 Gene3D IPR016040 NAD(P)-binding domain 569 708 100
SSF51735 SuperFamily 570 720 88
SSF53633 SuperFamily IPR001048 Aspartate/glutamate/uridylate kinase 67 344 3
SSF55347 SuperFamily 703 857 12
PIRSF036440 PIRSF IPR011241 NAGK-NAGSA, bifunctional 1 885 1
TIGR00761 tigrfam IPR004662 Acetylglutamate kinase 94 319 1
TIGR01850 tigrfam IPR000706 N-acetyl-gamma-glutamyl-phosphate reductase, type 1 572 883 1

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0100011cleaved_peptide_region295

Protein Properties

Ave. residue weight 110.40 Da
Charge 30.00
Isoelectric point 9.77
Molecular weight 97.70 kDa
Number of residues 885
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS836
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S836 PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
64389during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
70759during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
67388during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
63584during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
26527.97during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
67667during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
33200.01during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8.5during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
3.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Genome Organisation
DescriptionQualifierReferenceCount
divergently orientated to functionally related gene10
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in bacteria1001
conserved in metazoa3424
conserved in vertebrates3399
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withapc10anaphase-promoting complex substrate recognition subunit Apc10 Negative GeneticPMID:22681890
negative genetic interaction witharp8actin-like protein, Ino80 complex subunit Arp8 Negative GeneticPMID:22681890
negative genetic interaction withatp11mitochondrial F1-ATPase chaperone Atp11 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withbun107WD repeat protein, human WDR48 family Bun107 Negative GeneticPMID:22681890
negative genetic interaction withcdc37Hsp90 co-chaperone Cdc37 Negative GeneticPMID:22681890
negative genetic interaction withcdk11serine/threonine protein kinase cdk11 Negative GeneticPMID:22681890
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
negative genetic interaction withdsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
negative genetic interaction withdsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:22681890
negative genetic interaction withdsc4Golgi Dsc E3 ligase complex subunit Dsc4 Negative GeneticPMID:21504829
negative genetic interaction withebs1EST1 family nonsense-mediated mRNA decay (NMD) pathway protein Ebs1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withelp2elongator complex subunit Elp2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withelp3elongator complex subunit Elp3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withfar10SIP/FAR complex FHA domain subunit Far10/Csc1 Negative GeneticPMID:22681890
negative genetic interaction withgcn20AAA family ATPase Gcn20 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withgim6prefoldin subunit 1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withint6eIF3e subunit Int6 Negative GeneticPMID:22681890
positive genetic interaction withlsb5cortical component Lsb5 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withmcs2TFIIH complex cyclin Mcs2 Negative GeneticPMID:22681890
negative genetic interaction withmlo3RNA binding protein Mlo3 Negative GeneticPMID:22681890
negative genetic interaction withmpr1histidine-containing response regulator phosphotransferase Mpr1 Negative GeneticPMID:22681890
negative genetic interaction withpab2poly(A) binding protein Pab2 Negative GeneticPMID:22681890
negative genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Negative GeneticPMID:22681890
negative genetic interaction withphp5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
negative genetic interaction withpmc1mediator complex subunit Pmc1 Negative GeneticPMID:22681890
negative genetic interaction withpof9F-box protein Pof9 Negative GeneticPMID:22681890
positive genetic interaction withppk16serine/threonine protein kinase Ppk16 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withppm1leucine carboxyl methyltransferase, involved in regulation of autophagy Ppm1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withpub3HECT-type ubiquitin-protein ligase E3 Pub3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withreb1RNA polymerase I transcription termination factor/ RNA polymerase II transcription factor Reb1 Negative GeneticPMID:22681890
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
negative genetic interaction withsaf4splicing associated factor Saf4 Negative GeneticPMID:22681890
negative genetic interaction withsdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Negative GeneticPMID:22681890
negative genetic interaction withsfp1transcription factor Sfp1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withski3Ski complex TPR repeat subunit Ski3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC18H10.05WD repeat protein, human WDR44 family Negative GeneticPMID:22681890
positive genetic interaction withSPBC336.05csmall RNA 2'-O-methyltransferase activity (predicted) Positive GeneticPMID:22681890
negative genetic interaction withsre1sterol regulatory element binding protein, transcription factor Sre1 Negative GeneticPMID:21504829
negative genetic interaction withsre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
positive genetic interaction withsty1MAP kinase Sty1 Positive GeneticPMID:22681890
negative genetic interaction withswd2Set1C complex subunit Swd2.1 Negative GeneticPMID:22681890
negative genetic interaction withtif38translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
positive genetic interaction withubp2ubiquitin C-terminal hydrolase Ubp2 Positive GeneticPMID:22681890
negative genetic interaction withubp4ubiquitin C-terminal hydrolase Ubp4 Negative GeneticPMID:22681890
negative genetic interaction withvps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC4G9.09c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC4G9.09c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC4G9.09c BioGRID Interaction Datasets
Expression Viewer SPAC4G9.09c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC4G9.09c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC4G9.09c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC4G9.09c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC4G9.09c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC4G9.09c Transcriptome Viewer (Bähler Lab)
GEO SPAC4G9.09c GEO profiles
PInt SPAC4G9.09c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC4G9.09c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC4G9.09c Fission yeast phenotypic data & analysis
Cyclebase SPAC4G9.09c.1 Cell Cycle Data
IntEnz2.7.2.8Integrated relational Enzyme database
Rhea2.7.2.8Annotated reactions database
IntEnz1.2.1.38Integrated relational Enzyme database
Rhea1.2.1.38Annotated reactions database
SPD / RIKEN47/47A09Orfeome Localization Data
KEGGMAP00220KEGG Pathway Database
UniProtKB/SwissProtP31318Protein arg11, mitochondrial
ModBaseP31318Database of comparative protein structure models
STRINGP31318Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593691N-acetyl-gamma-glutamyl-phosphate reductase/acetylglutamate kinase
RefSeq mRNANM_001019123972h- N-acetyl-gamma-glutamyl-phosphate reductase/acetylglutamate kinase (arg11), mRNA
European Nucleotide ArchiveCAA45132.1ENA Protein Mapping
European Nucleotide ArchiveCAA93559.1ENA Protein Mapping
MetaCycPWY-5154Arginine biosynthesis III
MetaCycPWY-7400Arginine biosynthesis IV (archaebacteria)
KEGG_Enzyme00330+1.2.1.38Arginine and proline metabolism
KEGG_Enzyme00330+1.2.1.38+2.7.2.8Arginine and proline metabolism
KEGG_Enzyme00330+2.3.1.1Arginine and proline metabolism
KEGG_Enzyme00330+2.7.2.8Arginine and proline metabolism
UniParcUPI0000125D42UniProt Archive
UniPathwayUPA00068Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1

Literature for arg11

Search: Europe PMC or PubMed

Release Version: PomBase:26_53 - 27 May 2015