SPAC4H3.01


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPAC4H3.01 Feature Typeprotein coding
Synonyms Name Description
ProductDNAJ domain protein Caj1/Djp1 type (predicted) Product Size392aa, 44.77 kDa
Genomic Location Chromosome I, 3821682-3824461 (2780nt); CDS:3822935-3824280 (1346nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
Hsp70 protein binding26
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

protein folding


Term NameCount
protein folding104
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell division site305
Annotation ExtensionEvidenceWith/FromReference
cell tip194
Annotation ExtensionEvidenceWith/FromReference
cytosol2315
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
sensitive to bortezomibSPAC4H3.01ΔNull256
viable vegetative cell populationSPAC4H3.01ΔNull3792

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologySPAC4H3.01ΔNull3092
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3821682..3823020, 3823059..3823131, 3823216..3823886, 3823932..3824461
Introns3823021..3823058, 3823132..3823215
mRNA3821682..3824461
5' UTR3821682..3822934PMID:21511999
CDS3822935..3823020, 3823059..3823131, 3823216..3823886, 3823932..3824280
3' UTR3824281..3824461PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF14308 Pfam IPR026894 DNAJ-containing protein, X-domain 172 373 2
PF00226 Pfam IPR001623 DnaJ domain 8 71 24
SM00271 SMART IPR001623 DnaJ domain 7 66 23
PS50076 Prosite Profiles IPR001623 DnaJ domain 8 74 24
PS00636 Prosite Patterns IPR018253 DnaJ domain, conserved site 51 70 14
PTHR24076 HMMPANTHER 1 115 7
1.10.287.110 Gene3D IPR001623 DnaJ domain 6 95 26
SSF46565 SuperFamily IPR001623 DnaJ domain 7 101 25
Coil ncoils Rabaptin coiled-coil domain 163 195 968
PR00625 PRINTS IPR001623 DnaJ domain 10 28 21
PR00625 PRINTS IPR001623 DnaJ domain 66 85 21
PR00625 PRINTS IPR001623 DnaJ domain 28 43 21
PR00625 PRINTS IPR001623 DnaJ domain 46 66 21

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.21 Da
Charge -0.50
Isoelectric point 6.38
Molecular weight 44.77 kDa
Number of residues 392
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS293
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S293 PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
40094during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
40980during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
37145during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
40711during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
6889.4during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
39906during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
6331.21during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8.6during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byhhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
affinity captured byhhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withatb2tubulin alpha 2 Positive GeneticPMID:22681890
negative genetic interaction withddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
positive genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Positive GeneticPMID:22681890
negative genetic interaction withgcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
positive genetic interaction withhrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Positive GeneticPMID:22681890
positive genetic interaction withraf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Positive GeneticPMID:22681890
positive genetic interaction withsdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Positive GeneticPMID:22681890
negative genetic interaction withsfp1transcription factor Sfp1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsnf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
negative genetic interaction withSPBC11B10.08WW domain containing conserved fungal protein Negative GeneticPMID:22681890
negative genetic interaction withSPCC417.09ctranscription factor (predicted) Negative GeneticPMID:22681890
negative genetic interaction withswr1SNF2 family ATP-dependent DNA helicase Swr1 Negative GeneticPMID:22681890
negative genetic interaction withuch2ubiquitin C-terminal hydrolase Uch2 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC4H3.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC4H3.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC4H3.01 BioGRID Interaction Datasets
Expression Viewer SPAC4H3.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC4H3.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC4H3.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC4H3.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC4H3.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC4H3.01 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC4H3.01 Cell Cycle Data
GEO SPAC4H3.01 GEO profiles
PInt SPAC4H3.01 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC4H3.01 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC4H3.01 Fission yeast phenotypic data & analysis
SPD / RIKEN20/20G10Orfeome Localization Data
UniProtKB/SwissProtQ10209Uncharacterized J domain-containing protein C4H3.01
ModBaseQ10209Database of comparative protein structure models
STRINGQ10209Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594337DNAJ domain protein Caj1/Djp1 type (predicted)
RefSeq mRNANM_001019758972h- DNAJ domain protein Caj1/Djp1 type (predicted) (SPAC4H3.01), mRNA
RefSeq mRNAXM_004001737972h- hypothetical protein (SPAC4H3.17), mRNA
RefSeq PeptideXP_004001786hypothetical protein SPAC4H3.17
European Nucleotide ArchiveCAA93340.1ENA Protein Mapping
UniParcUPI000013A191UniProt Archive

Literature for SPAC4H3.01

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015