SPAC4H3.01


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPAC4H3.01 Feature Typeprotein coding
Synonyms Name Description
ProductDNAJ domain protein Caj1/Djp1 type (predicted) Product Size392aa, 44.77 kDa
Genomic Location Chromosome I, 3821682-3824461 (2780nt); CDS:3822935-3824280 (1346nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030544Hsp70 protein bindingISMPFAM:PF00226GO_REF:000000126
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006457protein foldingNASGO_REF:0000051111
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032153cell division siteIDAPMID:16823372296
GO:0051286cell tipIDAPMID:16823372189
GO:0005829cytosolIDAPMID:168233722316
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001701sensitive to bortezomib256
expressivity FYPO_EXT:0000003Cell growth assaySPAC4H3.01ΔNullPECO:0000005, PECO:0000137PMID:21760946
FYPO:0002060viable vegetative cell populationMicroscopySPAC4H3.01ΔNullPMID:204732893730
MicroscopySPAC4H3.01ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001MicroscopySPAC4H3.01ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
138216823823020
238230593823131
338232163823886
438239323824461

UTRs

Region Coordinates Reference
five_prime_UTR3821682..3822934PMID:21511999
three_prime_UTR3824281..3824461PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF14308 Pfam IPR026894 DNAJ-containing protein, X-domain 172 373 2
PF00226 Pfam IPR001623 DnaJ domain 8 71 24
SM00271 SMART IPR001623 DnaJ domain 7 66 23
PS50076 Prosite Profiles IPR001623 DnaJ domain 8 74 24
PS00636 Prosite Patterns IPR018253 DnaJ domain, conserved site 51 70 14
PTHR24076 HMMPANTHER 1 115 7
1.10.287.110 Gene3D IPR001623 DnaJ domain 6 95 26
SSF46565 SuperFamily IPR001623 DnaJ domain 7 101 25
Coil ncoils Rabaptin coiled-coil domain 163 195 968
PR00625 PRINTS IPR001623 DnaJ domain 66 85 21
PR00625 PRINTS IPR001623 DnaJ domain 10 28 21
PR00625 PRINTS IPR001623 DnaJ domain 28 43 21
PR00625 PRINTS IPR001623 DnaJ domain 46 66 21

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.21 Da
Charge -0.50
Isoelectric point 6.38
Molecular weight 44.77 kDa
Number of residues 392
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS293PMID:217125471669
present during mitotic M phase
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
40094during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
40980during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
37145during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
40711during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
39906during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
6889.4during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
6331.21during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
8.6during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
1.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4569
conserved in eukaryotes4482
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
uch2ubiquitin C-terminal hydrolase Uch2 Negative GeneticPMID:22681890
raf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Positive GeneticPMID:22681890
ddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Positive GeneticPMID:22681890
snf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Positive GeneticPMID:22681890
SPCC417.09ctranscription factor (predicted) Negative GeneticPMID:22681890
atb2tubulin alpha 2 Positive GeneticPMID:22681890
swr1SNF2 family helicase Swr1 Negative GeneticPMID:22681890
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
SPBC11B10.08WW domain containing conserved fungal protein Negative GeneticPMID:22681890
sfp1transcription factor Sfp1 (predicted) Negative GeneticPMID:22681890
sdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Positive GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPAC4H3.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC4H3.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC4H3.01 BioGRID Interaction Datasets
Expression Viewer SPAC4H3.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC4H3.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC4H3.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC4H3.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC4H3.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC4H3.01 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC4H3.01 Cell Cycle Data
GEO SPAC4H3.01 GEO profiles
PInt SPAC4H3.01 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC4H3.01 Peptides identified in tandem mass spectrometry proteomics experiments
WikiGene2543672DNAJ domain protein Caj1/Djp1 type
EntrezGene2543672DNAJ domain protein Caj1/Djp1 type
SPD / RIKEN20/20G10Orfeome Localization Data
UniProtKB/SwissProtQ10209Uncharacterized J domain-containing protein C4H3.01
ModBaseQ10209Database of comparative protein structure models
STRINGQ10209Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594337DNAJ domain protein Caj1/Djp1 type
RefSeq mRNANM_001019758972h- DNAJ domain protein Caj1/Djp1 type (SPAC4H3.01), mRNA
European Nucleotide ArchiveCAA93340ENA Protein Mapping
European Nucleotide ArchiveCAA93340.1ENA Protein Mapping
UniParcUPI000013A191UniProt Archive

Literature for SPAC4H3.01

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014