ppt1 (SPAC56F8.04c)


Gene Standard Nameppt1 Characterisation Statuspublished
Systematic IDSPAC56F8.04c Feature Typeprotein coding
Synonymscoq2 Name Description
Productpara-hydroxybenzoate--polyprenyltransferase Ppt1 Product Size360aa, 39.70 kDa
Genomic Location Chromosome I, 1134356-1133226 (1131nt); CDS:1134356-1133226 (1131nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:00020834-hydroxybenzoate decaprenyltransferase activityIEA EC:2.5.1.39GO_REF:00000031
GO:00472934-hydroxybenzoate nonaprenyltransferase activityIEA EC:2.5.1.39GO_REF:00000031
GO:00084124-hydroxybenzoate octaprenyltransferase activityIMPPMID:110928531
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0008299isoprenoid biosynthetic processIEAUniProtKB-KW:KW-0414GO_REF:000003715
GO:0006744ubiquinone biosynthetic processIMPPMID:1109285311
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0031305integral component of mitochondrial inner membraneICGO:0016021, GO:0005743GO_REF:000000114
GO:0005743mitochondrial inner membraneIDAPMID:11092853156
GO:0005739mitochondrionIDAPMID:16823372758
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001934abolished cell population growth on glycerol carbon sourceCell growth assayppt1ΔNullPECO:0000072PMID:1866526862
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assayppt1ΔNullPECO:0000126, PECO:0000102PMID:11092853119
FYPO:0001355decreased vegetative cell population growthCell growth assayppt1ΔNullPECO:0000126PMID:18665268342
FYPO:0000246growth auxotrophic for antioxidantCell growth assayppt1ΔNullPECO:0000183, PECO:0000103, PECO:0000126PMID:110928533
Cell growth assayppt1ΔNullPECO:0000179, PECO:0000126, PECO:0000102PMID:11092853
Cell growth assayppt1ΔNullPECO:0000118, PECO:0000126, PECO:0000102PMID:11092853
Cell growth assayppt1ΔNullPECO:0000005, PECO:0000179, PECO:0000126, PECO:0000102PMID:14695938
Cell growth assayppt1ΔNullPECO:0000005, PECO:0000179, PECO:0000126, PECO:0000102PMID:14695938
FYPO:0002061inviable vegetative cell populationCell growth assayppt1ΔNullPECO:0000005, PECO:0000126, PECO:0000102PMID:146959381337
FYPO:0000073resistance to caffeine20
expressivity FYPO_EXT:0000001Cell growth assayppt1ΔNullPECO:0000137PMID:19672306
FYPO:0001701sensitive to bortezomib256
expressivity FYPO_EXT:0000003Cell growth assayppt1ΔNullPECO:0000005, PECO:0000137PMID:21760946
FYPO:0000096sensitive to cadmium246
expressivity FYPO_EXT:0000001Cell growth assayppt1ΔNullPECO:0000102, PECO:0000005, PECO:0000137PMID:18684775
FYPO:0000103sensitive to copperCell growth assayppt1ΔNullPECO:0000103, PECO:0000137PMID:110928539
FYPO:0003559sensitive to doxorubicin91
expressivity FYPO_EXT:0000002Cell growth assayppt1ΔNullPECO:0000137, PECO:0000142PMID:23365689
FYPO:0000087sensitive to hydrogen peroxide124
expressivity FYPO_EXT:0000003Cell growth assayppt1ΔNullPECO:0000137PMID:19672306
Cell growth assayppt1ΔNullPECO:0000103, PECO:0000137PMID:11092853
expressivity FYPO_EXT:0000003Cell growth assayppt1ΔNullPECO:0000102, PECO:0000005, PECO:0000137PMID:18684775
Cell growth assayppt1ΔNullPECO:0000137PMID:18665268
FYPO:0000271sensitive to salt stress82
expressivity FYPO_EXT:0000003Cell growth assayppt1ΔNullPECO:0000207, PECO:0000137, PECO:0000102, PECO:0000005PMID:18684775
FYPO:0001234slow vegetative cell population growthCell growth assayppt1ΔNullPECO:0000137, PECO:0000103PMID:1109285374
FYPO:0002060viable vegetative cell populationMicroscopyppt1ΔNullPMID:204732893751
Microscopyppt1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001712coenzyme Q10 absent from cellChromatography evidenceppt1ΔNullPECO:0000126PMID:110928534
FYPO:0002723increased cellular pigment accumulation during cellular response to cadmium14
colour PATO:0000324Cell growth assayppt1ΔNullPECO:0000102, PECO:0000005, PECO:0000137PMID:18684775
FYPO:0001413increased cellular sulfide levelSubstance quantificationppt1ΔNullPECO:0000183, PECO:0000126PMID:110928538
Substance quantificationppt1ΔNullPMID:11092853
Substance quantificationppt1ΔNullPMID:11092853
FYPO:0000343increased hydrogen sulfide biosynthesisSubstance quantification evidenceppt1ΔNullPMID:146959384
Cell growth assayppt1ΔNullPMID:11092853
FYPO:0001489inviable vegetative cellCell growth assayppt1ΔNullPECO:0000126, PECO:0000103PMID:110928531195
FYPO:0001491viable vegetative cellCell growth assayppt1ΔNullPECO:0000005, PECO:0000118, PECO:0000126, PECO:0000102PMID:146959383599
Cell growth assayppt1ΔNullPECO:0000005, PECO:0000179, PECO:0000126, PECO:0000102PMID:14695938
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopyppt1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
111343561134332
211342831133226
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01040 Pfam IPR000537 UbiA prenyltransferase family 81 338 2
TMhelix TMHMM 200 222 959
TMhelix TMHMM 229 251 959
TMhelix TMHMM 281 303 959
PS00943 Prosite Patterns IPR000537 UbiA prenyltransferase family 127 149 2
PTHR11048 HMMPANTHER 2 359 2
PTHR11048:SF2 HMMPANTHER 2 359 1
TIGR01474 tigrfam IPR006370 4-hydroxybenzoate polyprenyl transferase 65 352 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.27 Da
Charge 14.50
Isoelectric point 9.70
Molecular weight 39.70 kDa
Number of residues 360
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2698.7during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.7during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.45during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by S. cerevisiae COQ2full PMID:11092853
Disease Association
DescriptionQualifierReferenceCount
coenzyme Q deficiency2
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in bacteria1002
conserved in metazoa3428
conserved in vertebrates3402
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
rad55RecA family ATPase Rad55/Rhp55 Phenotypic EnhancementPMID:23365689
dis2serine/threonine protein phosphatase PP1 subfamily, Dis2 Synthetic Growth DefectPMID:21965289
dad3DASH complex subunit Dad3 Phenotypic EnhancementPMID:23365689
Synthetic Growth Defect
External References
Database Identifier Description
NBRP SPAC56F8.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC56F8.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC56F8.04c BioGRID Interaction Datasets
Expression Viewer SPAC56F8.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC56F8.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC56F8.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC56F8.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC56F8.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC56F8.04c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC56F8.04c Cell Cycle Data
GEO SPAC56F8.04c GEO profiles
PInt SPAC56F8.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC56F8.04c Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN16/16F05Orfeome Localization Data
IntEnz2.5.1.-Integrated relational Enzyme database
Rhea2.5.1.-Annotated reactions database
UniProtKB/SwissProtQ102524-hydroxybenzoate polyprenyltransferase, mitochondrial
ModBaseQ10252Database of comparative protein structure models
STRINGQ10252Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593219para-hydroxybenzoate--polyprenyltransferase Ppt1
RefSeq mRNANM_001018615972h- para-hydroxybenzoate--polyprenyltransferase Ppt1 (ppt1), mRNA
European Nucleotide ArchiveABF50674.1ENA Protein Mapping
European Nucleotide ArchiveBAB20425.1ENA Protein Mapping
European Nucleotide ArchiveCAA93575.2ENA Protein Mapping
UniParcUPI0001B062B3UniProt Archive
UniPathwayUPA00232Cofactor biosynthesis; ubiquinone biosynthesis

Literature for ppt1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014