ppt1 (SPAC56F8.04c)


Gene Standard Nameppt1 Characterisation Statuspublished
Systematic IDSPAC56F8.04c Feature Typeprotein coding
Synonymscoq2 Name Description
Productpara-hydroxybenzoate--polyprenyltransferase Ppt1 Product Size360aa, 39.70 kDa
Genomic Location Chromosome I, 1134356-1133226 (1131nt); CDS:1134356-1133226 (1131nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
4-hydroxybenzoate decaprenyltransferase activity1
Annotation ExtensionEvidenceWith/FromReference
4-hydroxybenzoate nonaprenyltransferase activity1
Annotation ExtensionEvidenceWith/FromReference
4-hydroxybenzoate octaprenyltransferase activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
isoprenoid biosynthetic process16
Annotation ExtensionEvidenceWith/FromReference
ubiquinone biosynthetic process13
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
integral component of mitochondrial inner membrane18
Annotation ExtensionEvidenceWith/FromReference
mitochondrial inner membrane162
Annotation ExtensionEvidenceWith/FromReference
mitochondrion751
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viability depends on conditions

Population Phenotype

Term NameGenotypesCount
abolished cell population growth on glycerol carbon sourceppt1Δ67
decreased cell population growth on glucose carbon sourceppt1+319
ppt1Δ
decreased vegetative cell population growthppt1Δ657
growth auxotrophic for antioxidantppt1Δ3
inviable vegetative cell populationppt1Δ1451
resistance to caffeineppt1Δ26
sensitive to bortezomibppt1Δ255
sensitive to cadmiumppt1Δ249
sensitive to cisplatinppt1Δ106
sensitive to copperppt1Δ11
sensitive to doxorubicinppt1Δ95
sensitive to hydrogen peroxideppt1Δ154
sensitive to methyl methanesulfonateppt1Δ318
sensitive to salt stressppt1Δ98
slow vegetative cell population growthppt1Δ357
viable vegetative cell populationppt1Δ3840

Cell Phenotype

Term NameGenotypesCount
coenzyme Q10 absent from cellppt1Δ4
increased cellular pigment accumulation during cellular response to cadmium ion14
colour PATO:0000324ppt1Δ
increased cellular sulfide levelppt1Δ8
increased hydrogen sulfide biosynthesisppt1Δ4
inviable vegetative cellppt1Δ1351
viable vegetative cellppt1Δ3651
viable vegetative cell with normal cell morphologyppt1Δ3100

FYPO Multi-allele Phenotypes

Cell Phenotype

Term NameGenotypes
normal cellular coenzyme Q10 levelcoq5+, ppt1+, coq4+, coq6+, coq7+, coq9+, dps1+, dlp1+, abc1+, coq3+
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1134356..1134332, 1134283..1133226
Intron1134331..1134284
mRNA1134356..1133226
CDS1134356..1134332, 1134283..1133226
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01040 Pfam IPR000537 UbiA prenyltransferase family 89 337 2
TMhelix TMHMM 229 251 953
TMhelix TMHMM 281 303 953
TMhelix TMHMM 200 222 953
PS00943 Prosite Patterns IPR030470 UbiA prenyltransferase conserved site 127 149 2
PTHR11048 HMMPANTHER 38 354 2
PTHR11048:SF28 HMMPANTHER 38 354 1
MF_01635 HAMAP IPR031103 4-hydroxybenzoate octaprenyltransferase 60 352 1
TIGR01474 TIGRFAM IPR006370 4-hydroxybenzoate polyprenyltransferase 65 352 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.27 Da
Charge 14.50
Codon Adaptation Index 0.39
Isoelectric point 9.70
Molecular weight 39.70 kDa
Number of residues 360
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for ppt1 (SPAC56F8.04c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2698.7during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.7during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.45during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by S. cerevisiae COQ2full PMID:11092853
Disease Association
DescriptionQualifierReferenceCount
coenzyme Q deficiency3
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in bacteria1001
conserved in metazoa3424
conserved in vertebrates3399
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC56F8.04c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withmsc1Swr1 complex subunit Chk1 Two-hybridPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC56F8.04c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withtop2DNA topoisomerase II Synthetic Growth DefectPMID:25669599
synthetic growth defect withdis2serine/threonine protein phosphatase PP1, Dis2 Synthetic Growth DefectPMID:21965289
synthetic growth defect withdad3DASH complex subunit Dad3 Synthetic Growth DefectPMID:23365689
phenotype enhanced bydad3DASH complex subunit Dad3 Phenotypic EnhancementPMID:23365689
enhances phenotype ofrad55RecA family ATPase Rad55/Rhp55 Phenotypic EnhancementPMID:23365689
External References
Database Identifier Description
NBRP SPAC56F8.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC56F8.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC56F8.04c BioGRID Interaction Datasets
Expression Viewer SPAC56F8.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC56F8.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC56F8.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC56F8.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC56F8.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC56F8.04c Transcriptome Viewer (Bähler Lab)
GEO SPAC56F8.04c GEO profiles
PInt SPAC56F8.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC56F8.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC56F8.04c Fission yeast phenotypic data & analysis
Cyclebase SPAC56F8.04c.1 Cell Cycle Data
SPD / RIKEN16/16F05Orfeome Localization Data
IntEnz2.5.1.-Integrated relational Enzyme database
Rhea2.5.1.-Annotated reactions database
UniProtKB/SwissProtQ102524-hydroxybenzoate polyprenyltransferase, mitochondrial
ModBaseQ10252Database of comparative protein structure models
STRINGQ10252Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593219para-hydroxybenzoate--polyprenyltransferase Ppt1
RefSeq mRNANM_001018615972h- para-hydroxybenzoate--polyprenyltransferase Ppt1 (ppt1), mRNA

Literature for ppt1

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016