ppt1 (SPAC56F8.04c)

Gene Standard Nameppt1 Characterisation Statuspublished
Systematic IDSPAC56F8.04c Feature Typeprotein coding
Synonymscoq2 Name Description
Productpara-hydroxybenzoate--polyprenyltransferase Ppt1 Product Size360aa, 39.70 kDa
Genomic Location Chromosome I, 1134356-1133226 (1131nt); CDS:1134356-1133226 (1131nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
4-hydroxybenzoate decaprenyltransferase activity1
Annotation ExtensionEvidenceWith/FromReference
4-hydroxybenzoate nonaprenyltransferase activity1
Annotation ExtensionEvidenceWith/FromReference
4-hydroxybenzoate octaprenyltransferase activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
ubiquinone biosynthetic process14
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
integral component of mitochondrial inner membrane18
Annotation ExtensionEvidenceWith/FromReference
mitochondrial inner membrane160
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viability depends on conditions

Population Phenotype

Term NameGenotypesCount
abolished cell population growth on glycerol carbon sourceppt1Δ68
abolished cell population growth on glycerol/ethanol carbon sourceppt1Δ17
decreased cell population growth on glucose carbon sourceppt1Δ393
decreased vegetative cell population growthppt1Δ745
growth auxotrophic for antioxidantppt1Δ11
inviable vegetative cell populationppt1Δ1468
loss of viability in stationary phaseppt1Δ73
resistance to caffeineppt1Δ35
sensitive to bortezomibppt1Δ255
sensitive to cadmiumppt1Δ321
sensitive to cisplatinppt1Δ111
sensitive to cobaltppt1Δ115
sensitive to copperppt1Δ17
sensitive to doxorubicinppt1Δ97
sensitive to hydrogen peroxideppt1Δ160
sensitive to methyl methanesulfonateppt1Δ333
sensitive to salt stressppt1Δ102
slow vegetative cell population growthppt1Δ369
viable vegetative cell populationppt1Δ3862

Cell Phenotype

Term NameGenotypesCount
coenzyme Q10 absent from cellppt1Δ9
increased cellular pigment accumulation during cellular response to cadmium ion14
colour PATO:0000324ppt1Δ
increased cellular sulfide levelppt1Δ9
increased hydrogen sulfide biosynthesisppt1Δ4
inviable vegetative cellppt1Δ1377
viable vegetative cellppt1Δ3680
viable vegetative cell with normal cell morphologyppt1Δ3103

FYPO Multi-allele Phenotypes

Cell Phenotype

Term NameGenotypes
normal cellular coenzyme Q10 levelcoq6+, abc1+, coq4+, ppt1+, coq9+, dps1+, dlp1+, coq3+, coq5+, coq7+
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1134356..1134332, 1134283..1133226
CDS1134356..1134332, 1134283..1133226
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01040 Pfam IPR000537 UbiA prenyltransferase family 89 336 2
TMhelix TMHMM 229 251 950
TMhelix TMHMM 281 303 950
TMhelix TMHMM 200 222 950
PS00943 Prosite Patterns IPR030470 UbiA prenyltransferase conserved site 127 149 2
PTHR11048 HMMPANTHER 38 354 2
PTHR11048:SF28 HMMPANTHER 38 354 1
MF_01635 HAMAP IPR031103 4-hydroxybenzoate octaprenyltransferase 60 352 1
TIGR01474 TIGRFAM IPR006370 4-hydroxybenzoate polyprenyltransferase 65 352 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.27 Da
Charge 14.50
Codon Adaptation Index 0.39
Isoelectric point 9.70
Molecular weight 39.70 kDa
Number of residues 360
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for ppt1 (SPAC56F8.04c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2698.7during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.7during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633
0.45during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
functionally complemented by S. cerevisiae COQ2full PMID:11092853
functionally complemented by human COQ2 PMID:24911838
Disease Association
coenzyme Q deficiency3
Taxonomic Conservation
predominantly single copy (one to one)3087
conserved in fungi4608
conserved in eukaryotes4516
conserved in bacteria1005
conserved in metazoa3498
conserved in vertebrates3473

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC56F8.04c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withmsc1Swr1 complex subunit Chk1 Two-hybridPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC56F8.04c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withtop2DNA topoisomerase II Synthetic Growth DefectPMID:25669599
synthetic growth defect withdad3DASH complex subunit Dad3 Synthetic Growth DefectPMID:23365689
synthetic growth defect withdis2serine/threonine protein phosphatase PP1, Dis2 Synthetic Growth DefectPMID:21965289
enhances phenotype ofrad55RecA family ATPase Rad55/Rhp55 Phenotypic EnhancementPMID:23365689
phenotype enhanced bydad3DASH complex subunit Dad3 Phenotypic EnhancementPMID:23365689
External References
Database Identifier Description
NBRP SPAC56F8.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC56F8.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC56F8.04c BioGRID Interaction Datasets
Expression Viewer SPAC56F8.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC56F8.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC56F8.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC56F8.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC56F8.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC56F8.04c Transcriptome Viewer (Bähler Lab)
GEO SPAC56F8.04c GEO profiles
PInt SPAC56F8.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC56F8.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC56F8.04c Fission yeast phenotypic data & analysis
Cyclebase SPAC56F8.04c.1 Cell Cycle Data
SPD / RIKEN16/16F05Orfeome Localization Data
IntEnz2.5.1.-Integrated relational Enzyme database
Rhea2.5.1.-Annotated reactions database
UniProtKB/SwissProtQ102524-hydroxybenzoate polyprenyltransferase, mitochondrial
ModBaseQ10252Database of comparative protein structure models
STRINGQ10252Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593219para-hydroxybenzoate--polyprenyltransferase Ppt1
RefSeq mRNANM_001018615972h- para-hydroxybenzoate--polyprenyltransferase Ppt1 (ppt1), mRNA
European Nucleotide ArchiveABF50674.1ENA Protein Mapping
European Nucleotide ArchiveBAB20425.1ENA Protein Mapping
European Nucleotide ArchiveCAA93575.2ENA Protein Mapping
UniParcUPI0001B062B3UniProt Archive

Literature for ppt1

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017