ura3 (SPAC57A10.12c)


Gene Standard Nameura3 Characterisation Statuspublished
Systematic IDSPAC57A10.12c Feature Typeprotein coding
Synonyms Name Description
Productdihydroorotate dehydrogenase Ura3 Product Size443aa, 48.30 kDa
Genomic Location Chromosome I, 1387063-1385357 (1707nt); CDS:1386841-1385510 (1332nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
dihydroorotate dehydrogenase activity1
Annotation ExtensionEvidenceWith/FromReference
dihydroorotate oxidase activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
'de novo' UMP biosynthetic process5
Annotation ExtensionEvidenceWith/FromReference
pyrimidine nucleobase metabolic process13
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
integral component of membrane1018
Annotation ExtensionEvidenceWith/FromReference
mitochondrion761
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
growth auxotrophic for uracilura3ΔNull5
resistance to etoposideura3+ (wild type)Overexpression14
viable vegetative cell populationura3ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with abnormal cell shapeura3ΔNull482
viable vegetative cell with normal cell morphologyura3ΔNull3094
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1387063..1385357
mRNA1387063..1385357
5' UTR1387063..1386842AU006700
CDS1386841..1385510
3' UTR1385509..1385357PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01180 Pfam IPR005720 Dihydroorotate dehydrogenase domain 105 430 1
PS00911 Prosite Patterns IPR001295 Dihydroorotate dehydrogenase, conserved site 140 159 1
PS00912 Prosite Patterns IPR001295 Dihydroorotate dehydrogenase, conserved site 382 402 1
PTHR11938 HMMPANTHER 69 441 3
3.20.20.70 Gene3D IPR013785 Aldolase-type TIM barrel 57 441 35
SSF51395 SuperFamily 71 200 10
SSF51395 SuperFamily 228 431 10
TIGR01036 tigrfam IPR005719 Dihydroorotate dehydrogenase, class 2 94 433 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 109.02 Da
Charge 22.50
Isoelectric point 10.23
Molecular weight 48.30 kDa
Number of residues 443
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS5
present during cellular response to thiabendazoleS168
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S5 PMID:21712547
present during cellular response to thiabendazole IDA S168 PMID:18257517
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA leveldecreased during GO:0071470expression microarray evidencePMID:12529438
decreased during GO:0034605expression microarray evidencePMID:12529438
decreased in presence of cadmium sulfate expression microarray evidencePMID:12529438
decreased during GO:0034599expression microarray evidencePMID:12529438
decreased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
49905during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
48560during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
53564during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
48076during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
50489during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
28276.27during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
6506.28during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
29during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
2.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complements S. cerevisiae URA1partial PMID:1409592
functionally complemented by human DHODHfull PMID:22692683
Disease Association
DescriptionQualifierReferenceCount
postaxial acrofacial dysostosis syndrome PMID:226926831
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in bacteria1001
conserved in metazoa3424
conserved in vertebrates3399
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withamk2AMP-activated protein kinase beta subunit Amk2 Positive GeneticPMID:22681890
negative genetic interaction withatg13autophagy associated protein kinase regulatory subunit Atg13 Negative GeneticPMID:22681890
negative genetic interaction withbob1prefoldin subunit 5 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withcdk11serine/threonine protein kinase cdk11 Positive GeneticPMID:22681890
negative genetic interaction withclr1SHREC complex subunit Clr1 Negative GeneticPMID:22681890
negative genetic interaction withclr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:22681890
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
negative genetic interaction withdsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:22681890
negative genetic interaction withdsc4Golgi Dsc E3 ligase complex subunit Dsc4 Negative GeneticPMID:21504829
negative genetic interaction withdsc5UBX domain containing protein required for Sre1 cleavage Negative GeneticPMID:22681890
negative genetic interaction withfkbp39FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withfta6Mis6-Sim4 complex Fta6 Negative GeneticPMID:22681890
negative genetic interaction withgma12alpha-1,2-galactosyltransferase Gma12 Negative GeneticPMID:22681890
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
negative genetic interaction withhrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:22681890
positive genetic interaction withlsk1P-TEFb-associated cyclin-dependent protein kinase Lsk1 Positive GeneticPMID:22681890
positive genetic interaction withmug80cyclin Clg1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withnrm1MBF complex corepressor Nrm1 Negative GeneticPMID:22681890
positive genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
negative genetic interaction withppk14serine/threonine protein kinase Ppk14 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withprp1U4/U6 x U5 tri-snRNP complex subunit Prp1 Positive GeneticPMID:22681890
negative genetic interaction withprp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
negative genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
negative genetic interaction withptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
negative genetic interaction withpyp1tyrosine phosphatase Pyp1 Negative GeneticPMID:22681890
negative genetic interaction withraf2Rik1-associated factor Raf2 Negative GeneticPMID:22681890
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
negative genetic interaction withrik1silencing protein Rik1 Negative GeneticPMID:22681890
negative genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withrsv2transcription factor Rsv2 Positive GeneticPMID:22681890
negative genetic interaction withSPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:22681890
positive genetic interaction withSPBPB10D8.02carylsulfatase (predicted) Positive GeneticPMID:22681890
negative genetic interaction withSPCC1450.09cphospholipase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsty1MAP kinase Sty1 Negative GeneticPMID:22681890
negative genetic interaction withsyj1inositol-polyphosphate 5-phosphatase (synaptojanin homolog 1) Negative GeneticPMID:22681890
negative genetic interaction withtom70mitochondrial TOM complex subunit Tom70 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withtop1DNA topoisomerase I Negative GeneticPMID:22681890
negative genetic interaction withtsc2tuberin Negative GeneticPMID:22681890
rescuesura4orotidine 5'-phosphate decarboxylase Ura4 Phenotypic SuppressionPMID:23555823
synthetically rescuesura4orotidine 5'-phosphate decarboxylase Ura4 Synthetic RescuePMID:23555823
negative genetic interaction withvps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
negative genetic interaction withvps5retromer complex subunit Vps5 Negative GeneticPMID:22681890
negative genetic interaction withypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC57A10.12c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC57A10.12c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC57A10.12c BioGRID Interaction Datasets
Expression Viewer SPAC57A10.12c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC57A10.12c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC57A10.12c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC57A10.12c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC57A10.12c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC57A10.12c Transcriptome Viewer (Bähler Lab)
GEO SPAC57A10.12c GEO profiles
PInt SPAC57A10.12c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC57A10.12c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC57A10.12c Fission yeast phenotypic data & analysis
Cyclebase SPAC57A10.12c.1 Cell Cycle Data
SPD / RIKEN20/20F02Orfeome Localization Data
IntEnz1.3.3.1Integrated relational Enzyme database
Rhea1.3.3.1Annotated reactions database
KEGGMAP00240KEGG Pathway Database
UniProtKB/SwissProtP32747Dihydroorotate dehydrogenase (quinone), mitochondrial
ModBaseP32747Database of comparative protein structure models
STRINGP32747Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593317dihydroorotate dehydrogenase Ura3
RefSeq mRNANM_001018748972h- dihydroorotate dehydrogenase Ura3 (ura3), mRNA
European Nucleotide ArchiveX65114ENA EMBL mapping
European Nucleotide ArchiveCAB08175.1ENA Protein Mapping
MetaCycPWY-5686UMP biosynthesis
KEGG_Enzyme00240+1.3.5.2Pyrimidine metabolism
UniParcUPI0000132CA0UniProt Archive
UniPathwayUPA00070Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3

Literature for ura3

Search: Europe PMC or PubMed

Release Version: PomBase:26_52 - 11 May 2015