ura3 (SPAC57A10.12c)


Gene Standard Nameura3 Characterisation Statuspublished
Systematic IDSPAC57A10.12c Feature Typeprotein coding
Synonyms Name Description
Productdihydroorotate dehydrogenase Ura3 Product Size443aa, 48.30 kDa
Genomic Location Chromosome I, 1387063-1385357 (1707nt); CDS:1386841-1385510 (1332nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004152dihydroorotate dehydrogenase activityIDAPMID:14095921
GO:0004158dihydroorotate oxidase activityIDAPMID:151969331
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0044205'de novo' UMP biosynthetic processIDAPMID:14095925
GO:0006206pyrimidine nucleobase metabolic processNASGO_REF:000005113
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0016021integral component of membraneIEAUniProtKB-KW:KW-0812GO_REF:00000371017
GO:0005739mitochondrionIDAPMID:1409592758
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001012growth auxotrophic for uracilCell growth assayura3ΔNullPMID:235558235
Cell growth assayura3ΔNullPECO:0000102PMID:22692683
FYPO:0002060viable vegetative cell populationMicroscopyura3ΔNullPECO:0000005, PECO:0000137PMID:236978063730
Microscopyura3ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopyura3ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
113870631385357

UTRs

Region Coordinates Reference
three_prime_UTR1385509..1385357PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01180 Pfam IPR012135 Dihydroorotate dehydrogenase, class 1/ 2 105 430 1
PS00911 Prosite Patterns IPR001295 Dihydroorotate dehydrogenase, conserved site 140 159 1
PS00912 Prosite Patterns IPR001295 Dihydroorotate dehydrogenase, conserved site 382 402 1
PTHR11938 HMMPANTHER 69 441 3
3.20.20.70 Gene3D IPR013785 Aldolase-type TIM barrel 57 441 35
SSF51395 SuperFamily 71 200 10
SSF51395 SuperFamily 228 431 10
TIGR01036 tigrfam IPR005719 Dihydroorotate dehydrogenase, class 2 94 433 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 109.02 Da
Charge 22.50
Isoelectric point 10.23
Molecular weight 48.30 kDa
Number of residues 443
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineIDAS168PMID:182575171669
present during cellular response to thiabendazole
present during mitotic M phaseS5PMID:21712547
MOD:00696phosphorylated residueNASPMID:182575171921
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA leveldecreased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
decreased during GO:0071470expression microarray evidencePMID:12529438
decreased during GO:0034605expression microarray evidencePMID:12529438
decreased in presence of cadmium sulfate expression microarray evidencePMID:12529438
decreased during GO:0034599expression microarray evidencePMID:12529438

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
49905during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
48560during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
53564during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
48076during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
50489during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
28276.27during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
6506.28during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
29during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
2.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complements S. cerevisiae URA1partial PMID:1409592
functionally complemented by human DHODHfull PMID:22692683
Disease Association
DescriptionQualifierReferenceCount
postaxial acrofacial dysostosis syndrome PMID:226926831
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3393
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
vps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
ura4orotidine 5'-phosphate decarboxylase Ura4 Synthetic RescuePMID:23555823
Phenotypic Suppression
prp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
rik1silencing protein Rik1 Negative GeneticPMID:22681890
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
clr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:22681890
pyp1tyrosine phosphatase Pyp1 Negative GeneticPMID:22681890
SPCC1450.09cphospholipase (predicted) Negative GeneticPMID:22681890
lsk1P-TEFb-associated cyclin-dependent protein kinase Lsk1 Positive GeneticPMID:22681890
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
fta6Sim4 and Mal2 associated (4 and 2 associated) protein 6 Negative GeneticPMID:22681890
raf2Rik1-associated factor Raf2 Negative GeneticPMID:22681890
ppk14serine/threonine protein kinase Ppk14 (predicted) Negative GeneticPMID:22681890
sty1MAP kinase Sty1 Negative GeneticPMID:22681890
prp1U4/U6 x U5 tri-snRNP complex subunit Prp1 Positive GeneticPMID:22681890
SPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:22681890
dsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
cdk11serine/threonine protein kinase cdk11 Positive GeneticPMID:22681890
mug80cyclin Clg1 (predicted) Positive GeneticPMID:22681890
res1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
clr1cryptic loci regulator Clr1 Negative GeneticPMID:22681890
syj1inositol-polyphosphate 5-phosphatase (synaptojanin homolog 1) Negative GeneticPMID:22681890
rsv2transcription factor Rsv2 Positive GeneticPMID:22681890
tom70mitochondrial TOM complex subunit Tom70 (predicted) Negative GeneticPMID:22681890
ypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
rpl160160S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:22681890
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:22681890
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
nrm1MBF complex corepressor Nrm1 Negative GeneticPMID:22681890
hip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
top1DNA topoisomerase I Negative GeneticPMID:22681890
bob1prefoldin subunit 5 (predicted) Negative GeneticPMID:22681890
amk2AMP-activated protein kinase beta subunit Amk2 Positive GeneticPMID:22681890
atg13autophagy associated protein kinase regulatory subunit Atg13 Negative GeneticPMID:22681890
dsc5UBX domain containing protein required for Sre1 cleavage Negative GeneticPMID:22681890
SPBPB10D8.02carylsulfatase (predicted) Positive GeneticPMID:22681890
fkbp39FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) Negative GeneticPMID:22681890
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
prw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
prz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
vps5retromer complex subunit Vps5 Negative GeneticPMID:22681890
gma12alpha-1,2-galactosyltransferase Gma12 Negative GeneticPMID:22681890
tsc2tuberin Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPAC57A10.12c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC57A10.12c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC57A10.12c BioGRID Interaction Datasets
Expression Viewer SPAC57A10.12c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC57A10.12c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC57A10.12c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC57A10.12c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC57A10.12c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC57A10.12c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC57A10.12c Cell Cycle Data
GEO SPAC57A10.12c GEO profiles
PInt SPAC57A10.12c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC57A10.12c Peptides identified in tandem mass spectrometry proteomics experiments
WikiGene2542781dihydroorotate dehydrogenase Ura3
EntrezGene2542781dihydroorotate dehydrogenase Ura3
IntEnz1.3.3.1Integrated relational Enzyme database
Rhea1.3.3.1Annotated reactions database
KEGGMAP00240KEGG Pathway Database
SPD / RIKEN20/20F02Orfeome Localization Data
UniProtKB/SwissProtP32747Dihydroorotate dehydrogenase (quinone), mitochondrial
ModBaseP32747Database of comparative protein structure models
STRINGP32747Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593317dihydroorotate dehydrogenase Ura3
RefSeq mRNANM_001018748972h- dihydroorotate dehydrogenase Ura3 (ura3), mRNA
European Nucleotide ArchiveX65114ENA EMBL mapping
European Nucleotide ArchiveCAA46230ENA Protein Mapping
European Nucleotide ArchiveCAB08175ENA Protein Mapping
European Nucleotide ArchiveCAB08175.1ENA Protein Mapping
ReactomeREACT_227862
UniParcUPI0000132CA0UniProt Archive

Literature for ura3

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014