mip1 (SPAC57A7.11)


Gene Standard Namemip1 Characterisation Statuspublished
Systematic IDSPAC57A7.11 Feature Typeprotein coding
Synonyms Name DescriptionMei2 Interacting Protein
ProductWD repeat protein, Raptor homolog Mip1 Product Size1313aa, 148.53 kDa
Genomic Location Chromosome I, 1521460-1517223 (4238nt); CDS:1521290-1517349 (3942nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding887
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
positive regulation of conjugation with cellular fusion43
Annotation ExtensionEvidenceWith/FromReference
TOR signaling28
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4213
Annotation ExtensionEvidenceWith/FromReference
TORC1 complex7
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
abnormal mitotic cell cycle arrest with unreplicated DNAmip1-15 (1-173)57
decreased conjugation frequencymip1-15 (1-173)52
decreased spore germination frequencymip1Δ69
decreased sporulation frequencymip1+/mip1- (heterozygous diploid)103
inviable vegetative cell populationmip1-15 (1-173)1455
mip1Δ
slow vegetative cell population growthmip1-15 (1-173)367
viable vegetative cell populationmip1+3850

Cell Phenotype

Term NameGenotypesCount
abnormally arrested meiotic cell cyclemip1+/mip1- (heterozygous diploid)13
mip1-15 (1-173)
decreased RNA level during nitrogen starvation43
affecting mat2-Pcmip1-15 (1-173)
affecting mat2-Pimip1-15 (1-173)
elongated cellmip1-310819
increased protein level during vegetative growth114
affecting pyp2mip1-310
inviable after spore germination, multiple cell divisionsmip1Δ243
inviable after spore germination, single or double cell divisionmip1Δ107
inviable after spore germination, without cell division, with abnormal germ tube morphologymip1Δ153
inviable small vegetative cellmip1-15 (1-173)27
mip1-310

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
viable vegetative cell populationtor1+, mip1+
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1521460..1517223
mRNA1521460..1517223
5' UTR1521460..1521291PMID:21511999
CDS1521290..1517349
3' UTR1517348..1517223PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF14538 Pfam IPR029347 Raptor, N-terminal CASPase-like domain 114 265 1
SM00320 SMART IPR001680 WD40 repeat 1077 1117 112
SM00320 SMART IPR001680 WD40 repeat 1210 1249 112
SM00320 SMART IPR001680 WD40 repeat 1120 1161 112
SM00320 SMART IPR001680 WD40 repeat 1167 1207 112
SM00320 SMART IPR001680 WD40 repeat 1251 1299 112
SM00320 SMART IPR001680 WD40 repeat 1021 1065 112
SM01302 SMART IPR029347 Raptor, N-terminal CASPase-like domain 113 266 1
PS00678 Prosite Patterns IPR019775 WD40 repeat, conserved site 1194 1208 65
PS50294 Prosite Profiles IPR017986 WD40-repeat-containing domain 986 1258 104
PS50082 Prosite Profiles IPR001680 WD40 repeat 1174 1216 100
PS50082 Prosite Profiles IPR001680 WD40 repeat 1217 1249 100
PTHR12848 HMMPANTHER IPR004083 Regulatory associated protein of TOR 61 1311 1
PTHR12848:SF16 HMMPANTHER 61 1311 1
1.25.10.10 Gene3D IPR011989 Armadillo-like helical 529 736 86
2.130.10.10 Gene3D IPR015943 WD40/YVTN repeat-like-containing domain 1060 1305 145
2.130.10.10 Gene3D IPR015943 WD40/YVTN repeat-like-containing domain 983 1059 145
SSF48371 SuperFamily IPR016024 Armadillo-type fold 486 829 130
SSF50978 SuperFamily IPR017986 WD40-repeat-containing domain 994 1307 135
PR01547 PRINTS 466 496 1
PR01547 PRINTS 230 254 1
PR01547 PRINTS 544 560 1
PR01547 PRINTS 520 539 1
PR01547 PRINTS 203 228 1
PR01547 PRINTS 497 518 1
PR01547 PRINTS 156 175 1
PR01547 PRINTS 351 378 1
PR01547 PRINTS 313 333 1
PR01547 PRINTS 1145 1163 1
PR01547 PRINTS 112 133 1
PR01547 PRINTS 386 410 1
PR01547 PRINTS 177 198 1

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000190WD repeat proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000190

Protein Properties

Ave. residue weight 113.13 Da
Charge -3.50
Codon Adaptation Index 0.40
Isoelectric point 6.35
Molecular weight 148.53 kDa
Number of residues 1313
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during mitotic M phaseS847
present during mitotic M phaseS834
present during mitotic M phaseS837
present during mitotic M phaseS857
present during cellular response to thiabendazoleS1309
present during cellular response to thiabendazoleS874
present during mitotic M phaseS863
present during mitotic M phaseS859
S151, S834, S837, S857, S859, S863, S874, S882
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S847 PMID:21712547
present during mitotic M phase experimental evidence S834 PMID:21712547
present during mitotic M phase experimental evidence S837 PMID:21712547
present during mitotic M phase experimental evidence S857 PMID:21712547
present during cellular response to thiabendazole IDA S1309 PMID:18257517
present during cellular response to thiabendazole IDA S874 PMID:18257517
present during mitotic M phase experimental evidence S863 PMID:21712547
present during mitotic M phase experimental evidence S859 PMID:21712547
IDA S859 PMID:25720772
IDA S834 PMID:18076573
IDA S882 PMID:25720772
IDA S874 PMID:25720772
IDA S834 PMID:25720772
IDA S857 PMID:25720772
IDA S863 PMID:25720772
IDA S151 PMID:18076573
IDA S882 PMID:18076573
IDA S837 PMID:25720772
O-phospho-L-threonine 1085
present during mitotic M phaseT861
present during mitotic M phaseT836
present during mitotic M phaseT832
T830, T832
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T861 PMID:21712547
present during mitotic M phase experimental evidence T836 PMID:21712547
present during mitotic M phase experimental evidence T832 PMID:21712547
IDA T830 PMID:25720772
IDA T832 PMID:25720772
phosphorylated residue 2511
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
NAS PMID:18076567
O-phosphorylated residueS834,S837, S834,S837,S847, S834,S847, S834,T836, S863,S874, S870,S874, T830,T832, T832,S834, T832,S837,S847, T832,T836, T836,S837 2457
Annotation ExtensionEvidenceResidueReference
IDA S834,S837,S847 PMID:25720772
IDA S870,S874 PMID:25720772
IDA T830,T832 PMID:25720772
IDA T832,S834 PMID:25720772
IDA S834,T836 PMID:25720772
IDA S834,S847 PMID:25720772
IDA S834,S837 PMID:25720772
IDA S863,S874 PMID:25720772
IDA T832,T836 PMID:25720772
IDA T836,S837 PMID:25720772
IDA T832,S837,S847 PMID:25720772
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0071470expression microarray evidencePMID:12529438

Quantitative Gene Expression

View graphical display of gene expression data for mip1 (SPAC57A7.11)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
513during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
553during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
499during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
273.89during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1118during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.7during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3093
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC57A7.11 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturestor2serine/threonine protein kinase Tor2 Affinity Capture-WesternPMID:17046992
affinity capturesmei2RNA-binding protein involved in meiosis Mei2 Affinity Capture-WesternPMID:10648609
affinity capturesste11transcription factor Ste11 Affinity Capture-WesternPMID:10648609
affinity captured bytor1serine/threonine protein kinase Tor1 Affinity Capture-WesternPMID:19417002
affinity captured bytor2serine/threonine protein kinase Tor2 Affinity Capture-WesternPMID:22645648
affinity captured bygtr1Gtr1/RagA G protein Gtr1 (predicted) Affinity Capture-WesternPMID:22344254
binds DNA-binding domain construct withmei2RNA-binding protein involved in meiosis Mei2 Two-hybridPMID:10648609
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC57A7.11 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetically rescuesmei2RNA-binding protein involved in meiosis Mei2 Synthetic RescuePMID:10648609
synthetically rescuesptr1HECT-type ubiquitin ligase E3 Ptr1 Synthetic RescuePMID:24766403
synthetically rescuespat1serine/threonine protein kinase Ran1/Pat1 Synthetic RescuePMID:10648609
overexpression rescuestor2serine/threonine protein kinase Tor2 Dosage RescuePMID:17261596
rescuesptr1HECT-type ubiquitin ligase E3 Ptr1 Phenotypic SuppressionPMID:24766403
rescuesago1argonaute Phenotypic SuppressionPMID:24766403
External References
Database Identifier Description
NBRP SPAC57A7.11 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC57A7.11 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC57A7.11 BioGRID Interaction Datasets
Expression Viewer SPAC57A7.11 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC57A7.11 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC57A7.11 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC57A7.11 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC57A7.11 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC57A7.11 Transcriptome Viewer (Bähler Lab)
GEO SPAC57A7.11 GEO profiles
PInt SPAC57A7.11 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC57A7.11 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC57A7.11 Fission yeast phenotypic data & analysis
Cyclebase SPAC57A7.11.1 Cell Cycle Data
SPD / RIKEN39/39D06Orfeome Localization Data
UniProtKB/SwissProtP87141WD repeat-containing protein mip1
ModBaseP87141Database of comparative protein structure models
STRINGP87141Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593370WD repeat-containing protein
RefSeq mRNANM_001018802972h- WD repeat-containing protein (mip1), mRNA
European Nucleotide ArchiveBAA84585.1ENA Protein Mapping
European Nucleotide ArchiveCAB08769.1ENA Protein Mapping
UniParcUPI000012F14BUniProt Archive

Literature for mip1

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016