dam1 (SPAC589.08c)


Gene Standard Namedam1 Characterisation Statuspublished
Systematic IDSPAC589.08c Feature Typeprotein coding
Synonyms Name Description
ProductDASH complex subunit Dam1 Product Size155aa, 17.44 kDa
Genomic Location Chromosome I, 3106934-3106191 (744nt); CDS:3106841-3106374 (468nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
attachment of mitotic spindle microtubules to kinetochore34
Annotation ExtensionEvidenceWith/FromReference
cellular response to osmotic stress35
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle organization38
Annotation ExtensionEvidenceWith/FromReference
sister chromatid biorientation16
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
condensed chromosome, centromeric region75
Annotation ExtensionEvidenceWith/FromReference
condensed nuclear chromosome kinetochore59
Annotation ExtensionEvidenceWith/FromReference
cytosol2315
Annotation ExtensionEvidenceWith/FromReference
DASH complex10
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle257
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle pole body216
Annotation ExtensionEvidenceWith/FromReference
nucleus2740
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000096sensitive to cadmium246
expressivity FYPO_EXT:0000003Cell growth assaydam1ΔNullPECO:0000102, PECO:0000005, PECO:0000137PMID:18684775
FYPO:0000088sensitive to hydroxyurea514
expressivity FYPO_EXT:0000003Cell Growth Assaydam1ΔNullPECO:0000137, PECO:0000102PMID:23697806
FYPO:0002060viable vegetative cell populationMicroscopydam1ΔNullPMID:204732893759
Microscopydam1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopydam1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
GO localized by ask1 DASH complex subunit Ask1 PMID:16079915
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
131069343106191

UTRs

Region Coordinates Reference
five_prime_UTR3106934..3106842PMID:21511999
three_prime_UTR3106373..3106191PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08653 Pfam IPR013962 DASH complex subunit Dam1 31 88 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.54 Da
Charge -6.50
Isoelectric point 4.55
Molecular weight 17.44 kDa
Number of residues 155
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.63during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.18during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only623
conserved in fungi4600
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
duo1DASH complex subunit Duo1 Affinity Capture-MSPMID:16079914
Two-hybridPMID:23695164
dad1DASH complex subunit Dad1 Affinity Capture-MSPMID:16079914
Two-hybridPMID:23695164
dad3DASH complex subunit Dad3 Affinity Capture-MSPMID:16079914
plo1Polo kinase Plo1 Biochemical ActivityPMID:22375062
spc34DASH complex subunit Spc34 Affinity Capture-MSPMID:16079914
Two-hybridPMID:23695164
ask1DASH complex subunit Ask1 Affinity Capture-MSPMID:16079914
Affinity Capture-WesternPMID:22375062
dad2DASH complex subunit Dad2 Affinity Capture-MSPMID:16079914
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
rps110240S ribosomal protein S11 (predicted) Negative GeneticPMID:22681890
mph1dual specificity protein kinase Mph1 Negative GeneticPMID:22681890
klp6kinesin-like protein Klp6 Synthetic LethalityPMID:16079915
SPCC16C4.106-phosphogluconolactonase (predicted) Negative GeneticPMID:22681890
not3CCR4-Not complex subunit Not3/5 (predicted) Negative GeneticPMID:22681890
dbr1RNA lariat debranching enzyme Dbr1 Negative GeneticPMID:22681890
ddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
SPBC1921.04cdubious Negative GeneticPMID:22681890
bob1prefoldin subunit 5 (predicted) Negative GeneticPMID:22681890
ash2Ash2-trithorax family protein Negative GeneticPMID:22681890
rad57RecA family ATPase Rad57/Rhp57 Negative GeneticPMID:22681890
pop2F-box/WD repeat protein Pop2 Negative GeneticPMID:22681890
SPCC70.03cproline dehydrogenase (predicted) Negative GeneticPMID:22681890
dis2serine/threonine protein phosphatase PP1 subfamily, Dis2 Synthetic Growth DefectPMID:22375062
vht1vitamin H transporter Vht1 Negative GeneticPMID:22681890
nse6Smc5-6 complex non-SMC subunit Nse6 Negative GeneticPMID:22681890
SPAC227.17cconserved protein Negative GeneticPMID:22681890
pof3F-box protein Pof3 Negative GeneticPMID:22681890
hhf2histone H4 h4.2 Negative GeneticPMID:22681890
SPAC9G1.08cphospholipase (predicted) Negative GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
mpd2GYF domain protein Negative GeneticPMID:22681890
rpl300160S ribosomal protein L30 (predicted) Negative GeneticPMID:22681890
lub1WD repeat protein Lub1 Negative GeneticPMID:22681890
vps3CORVET complex subunit, GTPase regulator Vps3 (predicted) Negative GeneticPMID:22681890
git5heterotrimeric G protein beta subunit Git5 Positive GeneticPMID:22681890
SPAC1610.02cmitochondrial ribosomal protein subunit L1 (predicted) Negative GeneticPMID:22681890
leo1RNA polymerase II associated Paf1 complex (predicted) Negative GeneticPMID:22681890
mad2mitotic spindle checkpoint protein Mad2 Negative GeneticPMID:22681890
Phenotypic SuppressionPMID:24239120
msc1multi-copy suppressor of Chk1 Negative GeneticPMID:22681890
mis6inner centromere protein, CENP-I ortholog Mis6 Synthetic LethalityPMID:16079915
atd1aldehyde dehydrogenase (predicted) Negative GeneticPMID:22681890
SPCC1840.05cphosphoglucomutase (predicted) Negative GeneticPMID:22681890
SPCC594.06cvacuolar SNARE Vam7 (predicted) Negative GeneticPMID:22681890
SPAC1296.01cphosphoacetylglucosamine mutase (predicted) Negative GeneticPMID:22681890
SPAC227.05prefoldin subunit 4 (predicted) Negative GeneticPMID:22681890
SPCC16C4.20cHMG box protein (predicted) Negative GeneticPMID:22681890
SPAC3A12.13ctranslation initiation factor eIF3j (p35) Negative GeneticPMID:22681890
atp14F1-ATPase subunit H (predicted) Negative GeneticPMID:22681890
gmh5alpha-1,2-galactosyltransferase (predicted) Negative GeneticPMID:22681890
arb1argonaute inhibitor protein 1 Negative GeneticPMID:22681890
dcd1deoxycytidylate deaminase (predicted) Negative GeneticPMID:22681890
tma20RNA-binding protein Tma20 (predicted) Negative GeneticPMID:22681890
rtf2replication termination factor Rtf2 Negative GeneticPMID:22681890
mgr2mitochondrial membrane protein Mgr2 (predicted) Negative GeneticPMID:22681890
pli1SUMO E3 ligase Pli1 Negative GeneticPMID:22681890
asp1inositol hexakisphosphate kinase/inositol pyrophosphate synthase (predicted) Negative GeneticPMID:22681890
cph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
spo20sec14 cytosolic factor family Sec14 Negative GeneticPMID:22681890
sck2serine/threonine protein kinase Sck2 Negative GeneticPMID:24463365
rpl200160S ribosomal protein L20a (predicted) Positive GeneticPMID:22681890
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Negative GeneticPMID:22681890
bdf2BET family double bromodomain protein Bdf2 Negative GeneticPMID:22681890
kap113karyopherin Kap113 Negative GeneticPMID:22681890
msd1mitotic-spindle disanchored Msd1 Negative GeneticPMID:22681890
pht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:22681890
pex13peroxin 13 (predicted) Positive GeneticPMID:22681890
SPAC1F5.03cFAD-dependent oxidoreductase involved in late endosome to Golgi transport (predicted) Negative GeneticPMID:22681890
SPCC550.11karyopherin (predicted) Negative GeneticPMID:22681890
SPAC13G6.09SSU-rRNA maturation protein Tsr4 homolog 2 (predicted) Negative GeneticPMID:22681890
deg1tRNA-pseudouridine synthase (predicted) Positive GeneticPMID:22681890
atp10F1-F0 ATPase assembly protein (predicted) Negative GeneticPMID:22681890
pac10prefoldin subunit 3 Pac10 (predicted) Negative GeneticPMID:22681890
rnc1RNA-binding protein that suppresses calcineurin deletion Rnc1 Negative GeneticPMID:22681890
csi2mitotic chromosome segregation protein Csi2 Negative GeneticPMID:22681890
set1histone lysine methyltransferase Set1 Negative GeneticPMID:22681890
rps250240S ribosomal protein S25 (predicted) Negative GeneticPMID:22681890
rtt109RTT109 family histone lysine acetyltransferase Negative GeneticPMID:22681890
clr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:22681890
mhf2FANCM-MHF complex subunit Mhf2 Negative GeneticPMID:22681890
rpa12DNA-directed RNA polymerase complex I subunit Rpa12 Negative GeneticPMID:22681890
csi1mitotic chromosome segregation protein Csi1 Synthetic LethalityPMID:23166349
sld3DNA replication pre-initiation complex subunit Sld3 Positive GeneticPMID:22681890
clp1Cdc14-related protein phosphatase Clp1/Flp1 Negative GeneticPMID:22681890
pmt3SUMO Negative GeneticPMID:22681890
mad3mitotic spindle checkpoint protein Mad3 Negative GeneticPMID:22681890
pam17TIM23 translocase complex-associated motor subunit Pam17 (predicted) Negative GeneticPMID:22681890
vps13achorein homolog (predicted) Negative GeneticPMID:22681890
ire1serine/threonine protein kinase, sensor for unfolded proteins in the ER Ire1 Negative GeneticPMID:22681890
apq12nuclear membrane organization protein Apq12 (predicted) Negative GeneticPMID:22681890
mal2kinetochore protein, CENP-O ortholog Mal2 Synthetic LethalityPMID:16079915
tls1splicing factor Tls1 Negative GeneticPMID:22681890
fin1serine/threonine protein kinase, NIMA related Fin1 Negative GeneticPMID:22681890
pmc2mediator complex subunit Pmc2/Med1 Negative GeneticPMID:22681890
snt2Lid2 complex subunit Snt2 Negative GeneticPMID:22681890
ptp4phosphatidate cytidylyltransferase Ptp4 (predicted) Negative GeneticPMID:22681890
SPBPB2B2.09c2-dehydropantoate 2-reductase (predicted) Negative GeneticPMID:22681890
erg28Erg28 protein (predicted) Negative GeneticPMID:22681890
rga8Rho-type GTPase activating protein Rga8 Negative GeneticPMID:22681890
SPCC18.17cproteasome assembly chaperone (predicted) Negative GeneticPMID:22681890
tos4FHA domain protein Tos4 (predicted) Negative GeneticPMID:22681890
reb1RNA polymerase I transcription termination factor/ RNA polymerase II transcription factor Reb1 Negative GeneticPMID:22681890
SPAC17H9.08mitochondrial coenzyme A transporter (predicted) Negative GeneticPMID:22681890
naa38NatC N-acetyltransferase non-catalytic Sm-like domain subunit Naa38 (predicted) Negative GeneticPMID:22681890
ulp2SUMO deconjugating cysteine peptidase Ulp2 (predicted) Negative GeneticPMID:22681890
SPBC32H8.01cconserved fungal protein, involved in endocytic trafficking (predicted) Negative GeneticPMID:22681890
mrf1mitochondrial peptide chain release factor (predicted) Negative GeneticPMID:22681890
SPCC736.07cunconventional prefoldin involved in translation initiation (predicted) Negative GeneticPMID:22681890
ubx2UBX domain protein Ubx2 Negative GeneticPMID:22681890
rps2340S ribosomal protein S23 (predicted) Negative GeneticPMID:22681890
rhp18Rad18 homolog ubiquitin protein ligase E3, Rhp18 Negative GeneticPMID:22681890
set3histone lysine methyltransferase Set3 Negative GeneticPMID:22681890
egd2nascent polypeptide-associated complex alpha subunit Egd2 Negative GeneticPMID:22681890
nop52nucleolar protein Nop52 family (predicted) Negative GeneticPMID:22681890
SPCC70.08cmethyltransferase (predicted) Negative GeneticPMID:22681890
SPAC222.08cglutamine aminotransferase subunit (predicted) Negative GeneticPMID:22681890
hat1histone acetyltransferase Hat1 Negative GeneticPMID:22681890
ura1carbamoyl-phosphate synthase (glutamine hydrolyzing), aspartate carbamoyltransferase Ura1 Positive GeneticPMID:22681890
erd1Erd1 homolog (predicted) Negative GeneticPMID:22681890
adn2transcription factor (predicted) Negative GeneticPMID:22681890
nem1Nem1-Spo7 phosphatase complex catalytic subunit Nem1 (predicted) Negative GeneticPMID:22681890
ask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
hrq1RecQ type DNA helicase Hrq1 (predicted) Negative GeneticPMID:22681890
alp16gamma tubulin complex subunit Alp16 Negative GeneticPMID:22681890
SPBC13E7.03cRNA hairpin binding protein (predicted) Negative GeneticPMID:22681890
swr1SNF2 family helicase Swr1 Negative GeneticPMID:22681890
mto1MT organizer Mto1 Negative GeneticPMID:22681890
rps40140S ribosomal protein S4 (predicted) Negative GeneticPMID:22681890
dsk1SR protein-specific kinase Dsk1 Negative GeneticPMID:22681890
ctp1CtIP-related endonuclease Negative GeneticPMID:22681890
SPCC584.01csulfite reductase NADPH flavoprotein subunit (predicted) Negative GeneticPMID:22681890
mms1E3 ubiquitin ligase complex subunit Mms1 (predicted) Negative GeneticPMID:22681890
rpt419S proteasome regulatory subunit Rpt4 (predicted) Negative GeneticPMID:22681890
SPAC24B11.12cP-type ATPase (predicted) Negative GeneticPMID:22681890
arp8actin-like protein, Ino80 complex subunit Arp8 Negative GeneticPMID:22681890
SPCC18.10pyridoxine-pyridoxal-pyridoxamine kinase (predicted) Negative GeneticPMID:22681890
klp5kinesin-like protein Klp5 Synthetic LethalityPMID:16079915
Synthetic Growth DefectPMID:22375062
but2But2 family protein But2 Negative GeneticPMID:22681890
SPBP35G2.14RNA-binding protein Negative GeneticPMID:22681890
ppk26protein kinase like PAN complex subunit Ppk26 Negative GeneticPMID:22681890
ies4Ino80 complex subunit Ies4 Negative GeneticPMID:22681890
bqt4bouquet formation protein Bqt4 Negative GeneticPMID:22681890
spc7kinetochore protein blinkin, Spc7 Synthetic LethalityPMID:22521786
psl1cyclin pho85 family Psl1 (predicted) Negative GeneticPMID:22681890
swc2Swr1 complex complex subunit Swc2 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC589.08c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC589.08c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC589.08c BioGRID Interaction Datasets
Expression Viewer SPAC589.08c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC589.08c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC589.08c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC589.08c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC589.08c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC589.08c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC589.08c Cell Cycle Data
GEO SPAC589.08c GEO profiles
PInt SPAC589.08c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC589.08c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC589.08c Fission yeast phenotypic data & analysis
SPD / RIKEN41/41C05Orfeome Localization Data
UniProtKB/SwissProtQ9HDZ6DASH complex subunit dam1
ModBaseQ9HDZ6Database of comparative protein structure models
STRINGQ9HDZ6Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594056DASH complex subunit Dam1
RefSeq mRNANM_001019480972h- DASH complex subunit Dam1 (dam1), mRNA
European Nucleotide ArchiveCAC19765.1ENA Protein Mapping
UniParcUPI00000699F3UniProt Archive

Literature for dam1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014