spo3 (SPAC607.10)

Gene Standard Namespo3 Characterisation Statuspublished
Systematic IDSPAC607.10 Feature Typeprotein coding
Synonyms Name Description
Productsporulation protein Spo3 Product Size1028aa, 119.41 kDa
Genomic Location Chromosome I, 2052799-2057177 (4379nt); CDS:2053945-2057031 (3087nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
ascospore-type prospore membrane assembly13
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
plasma membrane256
Annotation ExtensionEvidenceWith/FromReference
prospore membrane33
Annotation ExtensionEvidenceWith/FromReference
prospore membrane leading edge3
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
increased cell population growth ratespo3Δ256
normal vegetative cell population growth ratespo3Δ85
viable vegetative cell populationspo3Δ3862

Cell Phenotype

Term NameGenotypesCount
abnormal prospore membrane formationspo3Δ34
abolished sporulationspo3-B356
spo3m (KC51) (T427->opal)
normal meiosisspo3-B3151
normal meiosis Ispo3Δ9
normal meiosis IIspo3Δ8
normal protein localization to prospore membrane6
affecting spo3spo3m (KC51) (T427->opal)
affecting meu14spo3Δ
small sporesspo3Δ9
viable vegetative cell with normal cell morphologyspo3Δ3103

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased sporulation frequencyspo3Δ, psy1+ (wild type)
Target Of
FYPO affected by mutation in mei3 meiosis inducing protein Mei3
FYPO localization affected by mutation in npg1 GTPase regulator Rng2-like (predicted)
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR2052799..2053944PMID:21511999
3' UTR2057032..2057177PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
SignalP-noTM signalp 1 19 207

View domain organization at Pfam

Term IDTerm NameReferenceCount

Protein Properties

Ave. residue weight 116.16 Da
Charge 2.50
Codon Adaptation Index 0.39
Isoelectric point 6.75
Molecular weight 119.41 kDa
Number of residues 1028

Protein Modifications

Term NameResidueCount
O-phosphorylated residueT826,S827,T829 2461
Annotation ExtensionEvidenceResidueReference
IDA T826,S827,T829 PMID:25720772
Gene Expression

Qualitative Gene Expression

protein levelabsent during GO:0072690Western blot evidencePMID:11739793
increased during GO:0007135Western blot evidencePMID:11739793
absent during GO:0007127Western blot evidencePMID:11739793
RNA levelabsent during GO:0072690Northern assay evidencePMID:11739793
absent during GO:0007127Northern assay evidencePMID:11739793
increased during GO:0034293experimental evidencePMID:PMPB:0
increased during GO:0007135Northern assay evidencePMID:11739793

Quantitative Gene Expression

View graphical display of gene expression data for spo3 (SPAC607.10)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.1during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633
0.25during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
Schizosaccharomyces specific225

Orthologs in Compara

Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC607.10 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
rescuespsy1SNARE Psy1 Phenotypic SuppressionPMID:11739793
enhances phenotype ofdma1mitotic spindle checkpoint ubiquitin ligase Dma1 Phenotypic EnhancementPMID:20980623
positive genetic interaction withres1MBF transcription factor complex subunit Res1 Positive GeneticPMID:22681890
phenotype enhanced bynpg1GTPase regulator Rng2-like (predicted) Phenotypic EnhancementPMID:25146394
External References
Database Identifier Description
NBRP SPAC607.10 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC607.10 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC607.10 BioGRID Interaction Datasets
Expression Viewer SPAC607.10 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC607.10 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC607.10 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC607.10 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC607.10 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC607.10 Transcriptome Viewer (Bähler Lab)
GEO SPAC607.10 GEO profiles
PInt SPAC607.10 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC607.10 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC607.10 Fission yeast phenotypic data & analysis
Cyclebase SPAC607.10.1 Cell Cycle Data
SPD / RIKEN29/29H12Orfeome Localization Data
UniProtKB/SwissProtQ9US08Sporulation-specific protein 3
ModBaseQ9US08Database of comparative protein structure models
STRINGQ9US08Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593599sporulation protein Spo3
RefSeq mRNANM_001019030972h- sporulation protein Spo3 (spo3), mRNA
European Nucleotide ArchiveCAB63797.1ENA Protein Mapping
UniParcUPI0000135E5AUniProt Archive

Literature for spo3

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017