arp3 (SPAC630.03)


Gene Standard Namearp3 Characterisation Statuspublished
Systematic IDSPAC630.03 Feature Typeprotein coding
Synonymsact2 Name Description
ProductArp2/3 protein complex, actin-like protein subunit Arp3 Product Size427aa, 47.37 kDa
Genomic Location Chromosome I, 350406-352296 (1891nt); CDS:350477-352055 (1579nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
actin binding38
Annotation ExtensionEvidenceWith/FromReference
ATP binding559
Annotation ExtensionEvidenceWith/FromReference
protein binding863
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
actin cortical patch assembly22
Annotation ExtensionEvidenceWith/FromReference
actin cortical patch localization16
Annotation ExtensionEvidenceWith/FromReference
actin cortical patch organization24
Annotation ExtensionEvidenceWith/FromReference
actin filament polymerization19
Annotation ExtensionEvidenceWith/FromReference
Arp2/3 complex-mediated actin nucleation9
Annotation ExtensionEvidenceWith/FromReference
endocytosis76
Annotation ExtensionEvidenceWith/FromReference
mitotic actomyosin contractile ring assembly28
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actin cortical patch47
Annotation ExtensionEvidenceWith/FromReference
Arp2/3 protein complex7
Annotation ExtensionEvidenceWith/FromReference
cell cortex of cell tip44
Annotation ExtensionEvidenceWith/FromReference
cell division site313
Annotation ExtensionEvidenceWith/FromReference
cytoplasm4213
Annotation ExtensionEvidenceWith/FromReference
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
decreased cell population growth at high temperaturearp3-c1 (G334S)173
decreased cell population growth at low temperaturearp3-c1 (G334S)87
inviable vegetative cell populationarp3+1453
arp3Δ
viable vegetative cell populationarp3-c1 (G334S)3837

Cell Phenotype

Term NameGenotypesCount
abnormal actin cortical patch localization during mitosisarp3-c1 (G334S)3
binucleate monoseptate cell with mitotic cell cycle arrest before cell separationarp3-c1 (G334S)8
dispersed actin cortical patch localizationarp3-c1 (G334S)32
increased (1->3)-beta-D-glucan level at cell tiparp3-c1 (G334S)2
inviable after spore germination, without cell division, with abnormal cell morphology and abnormal actin cortical patch localizationarp3Δ1
inviable swollen sporearp3Δ3
loss of punctate cytoplasmic protein localization7
affects localization of arp3arp3-c1 (G334S)
mitotic cell cycle arrest before cell separationarp3-c1 (G334S)13
mitotic metaphase/anaphase transition delayarp3-c1 (G334S)19
normal actomyosin contractile ring assemblyarp3-c1 (G334S)16
normal protein level during vegetative growth78
affecting arp3arp3-c1 (G334S)
slow cell growth during recovery from nitrogen starvationarp3-c1 (G334S)1
vegetative cell lysisarp3+89
arp3Δ
Target Of
OntologyRelationshipGeneProduct
FYPO localization affected by mutation in arp3 Arp2/3 protein complex, actin-like protein subunit Arp3
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons350406..350511, 350575..350598, 350671..351199, 351295..351894, 351960..352296
Intron350512..350574, 350599..350670, 351200..351294, 351895..351959
5' UTR350406..350476PMID:21511999
3' UTR352056..352296PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00022 Pfam IPR004000 Actin family 7 419 11
SM00268 SMART IPR004000 Actin family 5 422 10
PS01132 Prosite Patterns IPR020902 Actin/actin-like conserved site 127 139 3
PTHR11937:SF31 HMMPANTHER 57 424 1
PTHR11937:SF31 HMMPANTHER 7 36 1
PTHR11937 HMMPANTHER IPR004000 Actin family 57 424 11
PTHR11937 HMMPANTHER IPR004000 Actin family 7 36 11
3.30.420.40 Gene3D Carbohydrate kinase, FGGY 7 217 24
3.30.420.40 Gene3D Carbohydrate kinase, FGGY 289 419 24
3.90.640.10 Gene3D Cell shape determining protein MreB/Mrl 218 288 16
SSF53067 SuperFamily 179 419 25
SSF53067 SuperFamily 6 167 25

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.94 Da
Charge -2.50
Codon Adaptation Index 0.48
Isoelectric point 6.07
Molecular weight 47.37 kDa
Number of residues 427
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for arp3 (SPAC630.03)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
106317during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
111538during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
107310during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
112432during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
32684.9during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
66700during GO:0072690PECO:0000012,
PECO:0000005
single cellexperimental evidencePMID:16224022
112579during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
35114.48during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
14during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2.7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4605
conserved in eukaryotes4515
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC630.03 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byarc1ARP2/3 actin-organizing complex subunit Sop2 Affinity Capture-WesternPMID:8978670
affinity captured bymyo1myosin type I Affinity Capture-MSPMID:16087707
affinity captured byrpn50119S proteasome regulatory subunit Rpn501 Affinity Capture-WesternPMID:20668161
affinity captured byarc3ARP2/3 actin-organizing complex subunit Arc21 Affinity Capture-WesternPMID:20668161
affinity captured bygmf1cofilin/tropomyosin family Glia Maturation Factor homolog Gmf1 Affinity Capture-WesternPMID:20517925
affinity captured byact1actin Act1 Affinity Capture-WesternPMID:9765059
co-purifies witharp2ARP2/3 actin-organizing complex subunit Arp2 Co-purificationPMID:18640983
co-purifies witharc1ARP2/3 actin-organizing complex subunit Sop2 Co-purificationPMID:10588653
forms complex witharp2ARP2/3 actin-organizing complex subunit Arp2 Reconstituted ComplexPMID:23148219
forms complex withact1actin Act1 Reconstituted ComplexPMID:23148219
forms complex withwsp1WASp homolog Reconstituted ComplexPMID:23148219
affinity capturesarc3ARP2/3 actin-organizing complex subunit Arc21 Affinity Capture-WesternPMID:21449051
affinity capturesarc1ARP2/3 actin-organizing complex subunit Sop2 Affinity Capture-WesternPMID:8978670
affinity capturesact1actin Act1 Affinity Capture-WesternPMID:9765059
co-fractionates witharc1ARP2/3 actin-organizing complex subunit Sop2 Co-fractionationPMID:8978670
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC630.03 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetically rescued bygmf1cofilin/tropomyosin family Glia Maturation Factor homolog Gmf1 Synthetic RescuePMID:20517925
enhances phenotype ofasp1inositol hexakisphosphate kinase/inositol pyrophosphate synthase (predicted) Phenotypic EnhancementPMID:20624911
rescued bygmf1cofilin/tropomyosin family Glia Maturation Factor homolog Gmf1 Phenotypic SuppressionPMID:20517925
synthetic lethal withact1actin Act1 Synthetic LethalityPMID:8978671
rescued by overexpression ofasp1inositol hexakisphosphate kinase/inositol pyrophosphate synthase (predicted) Dosage RescuePMID:10388810
synthetic growth defect withacp2F-actin capping protein beta subunit Acp2 Synthetic Growth DefectPMID:15743909
growth defect in presence of overexpressedgmf1cofilin/tropomyosin family Glia Maturation Factor homolog Gmf1 Dosage Growth DefectPMID:20517925
synthetically rescuescdc3profilin Synthetic RescuePMID:8978671
External References
Database Identifier Description
NBRP SPAC630.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC630.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC630.03 BioGRID Interaction Datasets
Expression Viewer SPAC630.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC630.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC630.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC630.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC630.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC630.03 Transcriptome Viewer (Bähler Lab)
GEO SPAC630.03 GEO profiles
PInt SPAC630.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC630.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC630.03 Fission yeast phenotypic data & analysis
Cyclebase SPAC630.03.1 Cell Cycle Data
SPD / RIKEN33/33C01Orfeome Localization Data
UniProtKB/SwissProtP32390Actin-related protein 3
ModBaseP32390Database of comparative protein structure models
STRINGP32390Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_592898actin-like protein Arp3
RefSeq mRNANM_001018298972h- actin-like protein Arp3 (arp3), mRNA

Literature for arp3

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016