arp3 (SPAC630.03)


Gene Standard Namearp3 Characterisation Statuspublished
Systematic IDSPAC630.03 Feature Typeprotein coding
Synonymsact2 Name Description
ProductArp2/3 protein complex, actin-like protein subunit Arp3 Product Size427aa, 47.37 kDa
Genomic Location Chromosome I, 350406-352296 (1891nt); CDS:350477-352055 (1579nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
actin binding38
Annotation ExtensionEvidenceWith/FromReference
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
protein binding856
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
actin cortical patch assembly22
Annotation ExtensionEvidenceWith/FromReference
actin cortical patch localization16
Annotation ExtensionEvidenceWith/FromReference
actin cortical patch organization24
Annotation ExtensionEvidenceWith/FromReference
actin filament polymerization21
Annotation ExtensionEvidenceWith/FromReference
Arp2/3 complex-mediated actin nucleation9
Annotation ExtensionEvidenceWith/FromReference
endocytosis78
Annotation ExtensionEvidenceWith/FromReference
mitotic actomyosin contractile ring assembly28
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actin cortical patch47
Annotation ExtensionEvidenceWith/FromReference
Arp2/3 protein complex7
Annotation ExtensionEvidenceWith/FromReference
cell cortex of cell tip44
Annotation ExtensionEvidenceWith/FromReference
cell division site305
Annotation ExtensionEvidenceWith/FromReference
cytoplasm4207
Annotation ExtensionEvidenceWith/FromReference
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growth at high temperaturearp3-c1 (G334S)Not specified134
inviable vegetative cell populationarp3+ (wild type)Overexpression1435
arp3ΔNull
sensitive to coldarp3-c1 (G334S)Not specified47
viable vegetative cell populationarp3-c1 (G334S)Not specified3795

Cell Phenotype

Term NameAlleleExpressionCount
abnormal actin cortical patch localization during mitosisarp3-c1 (G334S)Not specified3
binucleate monoseptate cell with mitotic cell cycle arrest before cell separationarp3-c1 (G334S)Not specified6
dispersed actin cortical patch localizationarp3-c1 (G334S)Not specified28
increased (1->3)-beta-D-glucan level at cell tiparp3-c1 (G334S)Not specified2
inviable swollen sporearp3ΔNull3
loss of punctate cytoplasmic protein localization5
affecting arp3arp3-c1 (G334S)Not specified
mitotic cell cycle arrest before cell separationarp3-c1 (G334S)Not specified10
mitotic metaphase/anaphase transition delayarp3-c1 (G334S)Not specified11
normal actomyosin contractile ring assemblyarp3-c1 (G334S)Not specified13
normal protein level during vegetative growth49
affecting arp3arp3-c1 (G334S)Not specified
slow cell growth during recovery from nitrogen starvationarp3-c1 (G334S)Not specified1
vegetative cell lysisarp3+ (wild type)Overexpression83
arp3ΔNull
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in arp3 Arp2/3 protein complex, actin-like protein subunit Arp3 PMID:8978671
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons350406..350511, 350575..350598, 350671..351199, 351295..351894, 351960..352296
mRNA350406..352296
5' UTR350406..350476PMID:21511999
CDS350477..350511, 350575..350598, 350671..351199, 351295..351894, 351960..352055
3' UTR352056..352296PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00022 Pfam IPR004000 Actin family 7 419 11
SM00268 SMART IPR004000 Actin family 5 422 10
PS01132 Prosite Patterns IPR020902 Actin/actin-like conserved site 127 139 3
3.30.420.40 Gene3D 7 217 24
3.90.640.10 Gene3D 218 288 16
3.30.420.40 Gene3D 289 419 24
SSF53067 SuperFamily 179 419 25
SSF53067 SuperFamily 6 167 25

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.94 Da
Charge -2.50
Isoelectric point 6.07
Molecular weight 47.37 kDa
Number of residues 427
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
106317during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
111538during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
107310during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
112432during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
112579during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
32684.9during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
66700during GO:0072690PECO:0000005,
PECO:0000012
single cellexperimental evidencePMID:16224022
35114.48during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
14during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
2.7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
forms complex withact1actin Act1 Reconstituted ComplexPMID:23148219
co-purifies witharc1ARP2/3 actin-organizing complex subunit Sop2 Co-purificationPMID:10588653
affinity capturesarc1ARP2/3 actin-organizing complex subunit Sop2 Affinity Capture-WesternPMID:8978670
affinity captured byarc1ARP2/3 actin-organizing complex subunit Sop2 Affinity Capture-WesternPMID:8978670
co-fractionates witharc1ARP2/3 actin-organizing complex subunit Sop2 Co-fractionationPMID:8978670
affinity captured byarc3ARP2/3 actin-organizing complex subunit Arc21 Affinity Capture-WesternPMID:21449051
affinity capturesarc3ARP2/3 actin-organizing complex subunit Arc21 Affinity Capture-WesternPMID:21449051
affinity captured byarc3ARP2/3 actin-organizing complex subunit Arc21 Affinity Capture-WesternPMID:20668161
co-purifies witharp2ARP2/3 actin-organizing complex subunit Arp2 Co-purificationPMID:18640983
forms complex witharp2ARP2/3 actin-organizing complex subunit Arp2 Reconstituted ComplexPMID:23148219
affinity captured bygmf1cofilin/tropomyosin family Glia Maturation Factor homolog Gmf1 Affinity Capture-WesternPMID:20517925
affinity captured bymyo1myosin type I Affinity Capture-MSPMID:16087707
affinity captured byrpn50119S proteasome regulatory subunit Rpn501 Affinity Capture-WesternPMID:20668161
forms complex withwsp1WASp homolog Reconstituted ComplexPMID:23148219
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withacp2F-actin capping protein beta subunit Acp2 Synthetic Growth DefectPMID:15743909
synthetic lethal withact1actin Act1 Synthetic LethalityPMID:8978671
rescued by overexpression ofasp1inositol hexakisphosphate kinase/inositol pyrophosphate synthase (predicted) Dosage RescuePMID:10388810
enhances phenotype ofasp1inositol hexakisphosphate kinase/inositol pyrophosphate synthase (predicted) Phenotypic EnhancementPMID:20624911
rescuescdc3profilin Phenotypic SuppressionPMID:8978671
rescued bygmf1cofilin/tropomyosin family Glia Maturation Factor homolog Gmf1 Phenotypic SuppressionPMID:20517925
synthetically rescued bygmf1cofilin/tropomyosin family Glia Maturation Factor homolog Gmf1 Synthetic RescuePMID:20517925
growth defect in presence of overexpressedgmf1cofilin/tropomyosin family Glia Maturation Factor homolog Gmf1 Dosage Growth DefectPMID:20517925
External References
Database Identifier Description
NBRP SPAC630.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC630.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC630.03 BioGRID Interaction Datasets
Expression Viewer SPAC630.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC630.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC630.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC630.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC630.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC630.03 Transcriptome Viewer (Bähler Lab)
GEO SPAC630.03 GEO profiles
PInt SPAC630.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC630.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC630.03 Fission yeast phenotypic data & analysis
Cyclebase SPAC630.03.1 Cell Cycle Data
SPD / RIKEN33/33C01Orfeome Localization Data
UniProtKB/SwissProtP32390Actin-related protein 3
ModBaseP32390Database of comparative protein structure models
STRINGP32390Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_592898actin-like protein Arp3
RefSeq mRNANM_001018298972h- actin-like protein Arp3 (arp3), mRNA
ePDB3DWLThe European PDB
PDB3DWLPDB
PDBsum3DWLPDBsum
European Nucleotide Archive-ENA Protein Mapping
European Nucleotide ArchiveCAB52725.1ENA Protein Mapping
UniParcUPI000012605DUniProt Archive

Literature for arp3

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015