arp3 (SPAC630.03)


Gene Standard Namearp3 Characterisation Statuspublished
Systematic IDSPAC630.03 Feature Typeprotein coding
Synonymsact2 Name Description
ProductArp2/3 protein complex, actin-like protein subunit Arp3 Product Size427aa, 47.37 kDa
Genomic Location Chromosome I, 350406-352296 (1891nt); CDS:350477-352055 (1579nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037559
GO:0003779actin bindingIEAUniProtKB-KW:KW-0009GO_REF:000003738
GO:0005515protein bindingIPIarc1PMID:8978670887
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0034314Arp2/3 complex-mediated actin nucleationIEAIPR015623GO_REF:00000027
GO:0000147actin cortical patch assemblyIMPPMID:1123157222
GO:0051666actin cortical patch localizationIMPPMID:1123157214
GO:0044396actin cortical patch organizationIMPPMID:89786715
GO:0030041actin filament polymerizationIMPPMID:112315725
GO:0000915cytokinesis, actomyosin contractile ring assemblyIMPPMID:1221423627
GO:0006897endocytosisICGO:0005885GO_REF:000000165
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005885Arp2/3 protein complexISSUniProtKB:P47117GO_REF:00000017
GO:0030479actin cortical patchIDAPMID:897867147
GO:0051285cell cortex of cell tipIDAPMID:897867138
GO:0032153cell division site292
during cytokinesisIDAPMID:8978671
GO:0005737cytoplasmIDAPMID:168233724198
GO:0005829cytosolIDAPMID:168233722317
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopyarp3ΔNullPECO:0000126PMID:89786711309
Microscopyarp3ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopyarp3ΔNullPMID:20473289
Microscopyarp3+ (wild type)OverexpressionPECO:0000126PMID:8978671
FYPO:0000080sensitive to coldCell growth assayarp3-c1 (G334S)Not specifiedPMID:897867130
FYPO:0002060viable vegetative cell populationCell growth assayarp3-c1 (G334S)Not specifiedPECO:0000005PMID:89786713755

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002373abnormal actin cortical patch localization during mitosisMicroscopyarp3-c1 (G334S)Not specifiedPMID:89786713
FYPO:0001224binucleate monoseptate cellMicroscopyarp3-c1 (G334S)Not specifiedPECO:0000006PMID:89786716
FYPO:0002021dispersed actin cortical patch localizationMicroscopyarp3-c1 (G334S)Not specifiedPECO:0000006PMID:897867115
FYPO:0002858increased (1->3)-beta-D-glucan level at cell tipMicroscopyarp3-c1 (G334S)Not specifiedPECO:0000006PMID:89786712
FYPO:0000049inviable cellCell growth assayarp3ΔNullPMID:17297221356
FYPO:0002207inviable swollen sporeMicroscopyarp3ΔNullPECO:0000005, PECO:0000137PMID:236978063
penetrance FYPO_EXT:0000001
FYPO:0002444loss of punctate cytoplasmic localizationMicroscopyarp3-c1 (G334S)Not specifiedPECO:0000006PMID:89786714
affecting arp3
FYPO:0002888mitotic cell cycle arrest before cell separationMicroscopyarp3-c1 (G334S)Not specifiedPECO:0000006PMID:89786711
FYPO:0000324mitotic metaphase/anaphase transition delayMicroscopyarp3-c1 (G334S)Not specifiedPECO:0000006PMID:89786713
FYPO:0001368normal actomyosin contractile ring assemblyMicroscopyarp3-c1 (G334S)Not specifiedPECO:0000006PMID:241272165
FYPO:0000833normal protein levelWestern blot assayarp3-c1 (G334S)Not specifiedPECO:0000006PMID:897867125
affecting arp3
FYPO:0002863slow cell growth during recovery from nitrogen starvationMicroscopyarp3-c1 (G334S)Not specifiedPECO:0000006PMID:89786711
FYPO:0000647vegetative cell lysisMicroscopyarp3+ (wild type)OverexpressionPECO:0000126PMID:897867136
Microscopyarp3ΔNullPECO:0000126PMID:8978671
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1350406350511
2350575350598
3350671351199
4351295351894
5351960352296

UTRs

Region Start End Reference
three_prime_UTR352056352296PMID:21511999
five_prime_UTR350406350476PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00022 Pfam IPR004000 Actin-related protein 7 419 11
SM00268 SMART IPR004000 Actin-related protein 5 422 10
PS01132 Prosite Patterns IPR020902 Actin/actin-like conserved site 127 139 3
PTHR11937 HMMPANTHER IPR004000 Actin-related protein 1 424 11
PTHR11937:SF31 HMMPANTHER IPR015623 1 424 1
G3DSA:3.90.640.10 Gene3D 218 288 16
G3DSA:3.30.420.40 Gene3D 7 217 24
G3DSA:3.30.420.40 Gene3D 289 419 24
SSF53067 SuperFamily 179 419 25
SSF53067 SuperFamily 6 167 25
Low complexity (SEG) seg 127 142
Low complexity (SEG) seg 39 53

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.94 Da
Charge -2.50
Isoelectric point 6.07
Molecular weight 47.37 kDa
Number of residues 427
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
32684.9during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
66700during GO:0072690PECO:0000005,
PECO:0000012
single_cellexperimental evidencePMID:16224022
35114.48during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2.7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
14during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
acp2F-actin capping protein beta subunit Acp2 Synthetic Growth DefectPMID:15743909
asp1inositol hexakisphosphate kinase/inositol pyrophosphate synthase (predicted) Dosage RescuePMID:10388810
Phenotypic EnhancementPMID:20624911
cdc3profilin Phenotypic SuppressionPMID:8978671
act1actin Act1 Synthetic LethalityPMID:8978671
gmf1cofilin/tropomyosin family Glia Maturation Factor homolog Gmf1 Synthetic RescuePMID:20517925
Phenotypic Suppression
Dosage Growth Defect
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
gmf1cofilin/tropomyosin family Glia Maturation Factor homolog Gmf1 Affinity Capture-WesternPMID:20517925
arp2ARP2/3 actin-organizing complex subunit Arp2 Co-purificationPMID:18640983
Reconstituted ComplexPMID:23148219
myo1myosin type I Affinity Capture-MSPMID:16087707
wsp1WASp homolog Reconstituted ComplexPMID:23148219
arc1ARP2/3 actin-organizing complex subunit Sop2 Co-purificationPMID:10588653
Affinity Capture-WesternPMID:8978670
Co-fractionation
rpn50119S proteasome regulatory subunit Rpn501 Affinity Capture-WesternPMID:20668161
arc3ARP2/3 actin-organizing complex subunit Arc21 Affinity Capture-WesternPMID:21449051
Affinity Capture-WesternPMID:20668161
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
act1actin Act1 Reconstituted ComplexPMID:23148219
External References
Database Identifier Description
NBRP SPAC630.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC630.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC630.03 BioGRID Interaction Datasets
Expression Viewer SPAC630.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC630.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC630.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC630.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC630.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC630.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC630.03 Cell Cycle Data
GEO SPAC630.03 GEO profiles
PInt SPAC630.03 Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2543233actin-like protein Arp3
EntrezGene2543233actin-like protein Arp3
SPD / RIKEN33/33C01Orfeome Localization Data
UniProtKB/SwissProtP32390Actin-related protein 3
ModBaseP32390Database of comparative protein structure models
StringP32390Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_592898actin-like protein Arp3
RefSeq mRNANM_001018298972h- actin-like protein Arp3 (arp3), mRNA
ePDB3DWLThe European PDB
PDBsum3DWLPDBsum
European Nucleotide ArchiveCAB52725ENA Protein Mapping
UniParcUPI000012605DUniProt Archive

Literature for arp3

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014