arp3 (SPAC630.03)


Gene Standard Namearp3 Characterisation Statuspublished
Systematic IDSPAC630.03 Feature Typeprotein coding
Synonymsact2 Name Description
ProductArp2/3 protein complex, actin-like protein subunit Arp3 Product Size427aa, 47.37 kDa
Genomic Location Chromosome I, 350406-352296 (1891nt); CDS:350477-352055 (1579nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037558
GO:0003779actin bindingIEAUniProtKB-KW:KW-0009GO_REF:000003738
GO:0005515protein bindingIPIarc1PMID:8978670831
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0034314Arp2/3 complex-mediated actin nucleationIEAIPR015623GO_REF:00000029
GO:0000147actin cortical patch assemblyIMPPMID:1123157222
GO:0051666actin cortical patch localizationIMPPMID:1123157216
GO:0044396actin cortical patch organizationIMPPMID:89786715
GO:0030041actin filament polymerizationIMPPMID:1123157221
GO:0006897endocytosisICGO:0005885GO_REF:000000176
GO:1903475mitotic actomyosin contractile ring assemblyIMPPMID:1221423627
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005885Arp2/3 protein complexISSUniProtKB:P47117GO_REF:00000247
GO:0030479actin cortical patchIDAPMID:897867147
GO:0051285cell cortex of cell tipIDAPMID:897867142
GO:0032153cell division site301
during cytokinesisIDAPMID:8978671
GO:0005737cytoplasmIDAPMID:168233724205
GO:0005829cytosolIDAPMID:168233722315
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000082decreased cell population growth at high temperature148
expressivity FYPO_EXT:0000001Cell growth assayarp3-c1 (G334S)Not specifiedPMID:10388810
FYPO:0002061inviable vegetative cell populationMicroscopyarp3ΔNullPECO:0000005, PECO:0000137PMID:236978061338
Microscopyarp3ΔNullPMID:20473289
Microscopyarp3ΔNullPECO:0000126PMID:8978671
Microscopyarp3+ (wild type)OverexpressionPECO:0000126PMID:8978671
FYPO:0000080sensitive to coldCell growth assayarp3-c1 (G334S)Not specifiedPMID:897867143
FYPO:0002060viable vegetative cell populationCell growth assayarp3-c1 (G334S)Not specifiedPECO:0000005PMID:89786713759

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002373abnormal actin cortical patch localization during mitosisMicroscopyarp3-c1 (G334S)Not specifiedPMID:89786713
FYPO:0003171binucleate monoseptate cell with mitotic cell cycle arrest before cell separationMicroscopyarp3-c1 (G334S)Not specifiedPECO:0000006PMID:89786715
FYPO:0002021dispersed actin cortical patch localizationMicroscopyarp3-c1 (G334S)Not specifiedPECO:0000006PMID:897867122
FYPO:0002858increased (1->3)-beta-D-glucan level at cell tipMicroscopyarp3-c1 (G334S)Not specifiedPECO:0000006PMID:89786712
FYPO:0000049inviable cellCell growth assayarp3ΔNullPMID:17297221449
FYPO:0002207inviable swollen spore3
penetrance FYPO_EXT:0000001Microscopyarp3ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0002444loss of punctate cytoplasmic protein localization5
affecting arp3Microscopyarp3-c1 (G334S)Not specifiedPECO:0000006PMID:8978671
FYPO:0002888mitotic cell cycle arrest before cell separationMicroscopyarp3-c1 (G334S)Not specifiedPECO:0000006PMID:89786717
FYPO:0000324mitotic metaphase/anaphase transition delayMicroscopyarp3-c1 (G334S)Not specifiedPECO:0000006PMID:89786718
FYPO:0001368normal actomyosin contractile ring assemblyMicroscopyarp3-c1 (G334S)Not specifiedPECO:0000006PMID:241272168
FYPO:0000833normal protein level40
affecting arp3Western blot assayarp3-c1 (G334S)Not specifiedPECO:0000006PMID:8978671
FYPO:0002863slow cell growth during recovery from nitrogen starvationMicroscopyarp3-c1 (G334S)Not specifiedPECO:0000006PMID:89786711
FYPO:0000647vegetative cell lysisMicroscopyarp3ΔNullPECO:0000126PMID:897867180
Microscopyarp3+ (wild type)OverexpressionPECO:0000126PMID:8978671
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in arp3 Arp2/3 protein complex, actin-like protein subunit Arp3 PMID:8978671
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1350406350511
2350575350598
3350671351199
4351295351894
5351960352296

UTRs

Region Coordinates Reference
five_prime_UTR350406..350476PMID:21511999
three_prime_UTR352056..352296PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00022 Pfam IPR004000 Actin-related protein 7 419 11
SM00268 SMART IPR004000 Actin-related protein 5 422 10
PS01132 Prosite Patterns IPR020902 Actin/actin-like conserved site 127 139 3
PTHR11937:SF31 HMMPANTHER IPR015623 Actin-related protein 3 (Arp3) 1 424 1
PTHR11937 HMMPANTHER IPR004000 Actin-related protein 1 424 11
3.30.420.40 Gene3D 7 217 24
3.90.640.10 Gene3D 218 288 16
3.30.420.40 Gene3D 289 419 24
SSF53067 SuperFamily 179 419 25
SSF53067 SuperFamily 6 167 25

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.94 Da
Charge -2.50
Isoelectric point 6.07
Molecular weight 47.37 kDa
Number of residues 427
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
106317during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
111538during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
107310during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
112432during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
66700during GO:0072690PECO:0000005,
PECO:0000012
single_cellexperimental evidencePMID:16224022
32684.9during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
112579during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
35114.48during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
14during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
2.7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
acp2F-actin capping protein beta subunit Acp2 Synthetic Growth DefectPMID:15743909
asp1inositol hexakisphosphate kinase/inositol pyrophosphate synthase (predicted) Dosage RescuePMID:10388810
Phenotypic EnhancementPMID:20624911
cdc3profilin Phenotypic SuppressionPMID:8978671
act1actin Act1 Synthetic LethalityPMID:8978671
gmf1cofilin/tropomyosin family Glia Maturation Factor homolog Gmf1 Dosage Growth DefectPMID:20517925
Phenotypic Suppression
Synthetic Rescue
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
gmf1cofilin/tropomyosin family Glia Maturation Factor homolog Gmf1 Affinity Capture-WesternPMID:20517925
arp2ARP2/3 actin-organizing complex subunit Arp2 Co-purificationPMID:18640983
Reconstituted ComplexPMID:23148219
myo1myosin type I Affinity Capture-MSPMID:16087707
wsp1WASp homolog Reconstituted ComplexPMID:23148219
arc1ARP2/3 actin-organizing complex subunit Sop2 Co-purificationPMID:10588653
Co-fractionationPMID:8978670
Affinity Capture-Western
rpn50119S proteasome regulatory subunit Rpn501 Affinity Capture-WesternPMID:20668161
arc3ARP2/3 actin-organizing complex subunit Arc21 Affinity Capture-WesternPMID:21449051
Affinity Capture-WesternPMID:20668161
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
act1actin Act1 Reconstituted ComplexPMID:23148219
External References
Database Identifier Description
NBRP SPAC630.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC630.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC630.03 BioGRID Interaction Datasets
Expression Viewer SPAC630.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC630.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC630.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC630.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC630.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC630.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC630.03 Cell Cycle Data
GEO SPAC630.03 GEO profiles
PInt SPAC630.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC630.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC630.03 Fission yeast phenotypic data & analysis
SPD / RIKEN33/33C01Orfeome Localization Data
UniProtKB/SwissProtP32390Actin-related protein 3
ModBaseP32390Database of comparative protein structure models
STRINGP32390Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_592898actin-like protein Arp3
RefSeq mRNANM_001018298972h- actin-like protein Arp3 (arp3), mRNA
ePDB3DWLThe European PDB
PDB3DWLPDB
PDBsum3DWLPDBsum
European Nucleotide Archive-ENA Protein Mapping
European Nucleotide ArchiveCAB52725.1ENA Protein Mapping
UniParcUPI000012605DUniProt Archive

Literature for arp3

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014