acp2 (SPAC631.01c)


Gene Standard Nameacp2 Characterisation Statuspublished
Systematic IDSPAC631.01c Feature Typeprotein coding
Synonyms Name Description
ProductF-actin capping protein beta subunit Acp2 Product Size268aa, 29.85 kDa
Genomic Location Chromosome I, 2105484-2104030 (1455nt); CDS:2105249-2104197 (1053nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
actin filament binding6
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
barbed-end actin filament capping2
Annotation ExtensionEvidenceWith/FromReference
mitotic actomyosin contractile ring contraction7
Annotation ExtensionEvidenceWith/FromReference
mitotic cytokinesis106
Annotation ExtensionEvidenceWith/FromReference
negative regulation of actin filament depolymerization3
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actin cortical patch47
Annotation ExtensionEvidenceWith/FromReference
actin filament5
Annotation ExtensionEvidenceWith/FromReference
cell division site313
Annotation ExtensionEvidenceWith/FromReference
cell tip204
Annotation ExtensionEvidenceWith/FromReference
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
F-actin capping protein complex2
Annotation ExtensionEvidenceWith/FromReference
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationacp2Δ3840

Cell Phenotype

Term NameGenotypesCount
elongated telomeresacp2Δ173
viable vegetative cell with normal cell morphologyacp2Δ3100
Target Of
OntologyRelationshipGeneProduct
GO localized by acp1 F-actin capping protein alpha subunit
GO substrate of ubp9 ubiquitin C-terminal hydrolase Ubp9
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2105484..2105244, 2105046..2104650, 2104600..2104030
Intron2105243..2105047, 2104649..2104601
mRNA2105484..2104030
5' UTR2105484..2105250PMID:21511999
CDS2105249..2105244, 2105046..2104650, 2104600..2104197
3' UTR2104196..2104030PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01115 Pfam IPR001698 F-actin-capping protein subunit beta 5 240 1
PS00231 Prosite Patterns IPR019771 F-actin capping protein, beta subunit, conserved site 60 65 1
PTHR10619 HMMPANTHER IPR001698 F-actin-capping protein subunit beta 1 266 1
SSF90096 SuperFamily 4 264 2
PR00192 PRINTS IPR001698 F-actin-capping protein subunit beta 95 122 1
PR00192 PRINTS IPR001698 F-actin-capping protein subunit beta 24 45 1
PR00192 PRINTS IPR001698 F-actin-capping protein subunit beta 55 81 1
PR00192 PRINTS IPR001698 F-actin-capping protein subunit beta 3 23 1
PR00192 PRINTS IPR001698 F-actin-capping protein subunit beta 212 238 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.36 Da
Charge -15.00
Codon Adaptation Index 0.48
Isoelectric point 4.26
Molecular weight 29.85 kDa
Number of residues 268
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phaseS267
S267
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S267 PMID:21712547
experimental evidence S267 PMID:24763107
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for acp2 (SPAC631.01c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
76444during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
86506during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
73343during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
84221during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
85939during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
19200during GO:0072690PECO:0000012,
PECO:0000005
single cellexperimental evidencePMID:16224022
13342during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
22037.39during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8.9during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.6during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2506
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC631.01c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
forms complex withstg1SM22/transgelin-like actin modulating protein Stg1 Reconstituted ComplexPMID:16256112
affinity capturesacp1F-actin capping protein alpha subunit Affinity Capture-WesternPMID:16866873
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC631.01c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
overexpression lethal tocdc12formin Cdc12 Dosage LethalityPMID:15743909
synthetic growth defect withfim1fimbrin Synthetic Growth DefectPMID:16866873
synthetic growth defect withrng2IQGAP Synthetic Growth DefectPMID:15743909
synthetic growth defect withcdc4myosin II light chain Synthetic Growth DefectPMID:15743909
synthetic growth defect withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Synthetic Growth DefectPMID:18931302
synthetic growth defect withmid1medial ring protein Mid1 Synthetic Growth DefectPMID:15743909
synthetic growth defect witharp3Arp2/3 protein complex, actin-like protein subunit Arp3 Synthetic Growth DefectPMID:15743909
synthetic growth defect withcrn1actin binding protein, coronin Crn1 Synthetic Growth DefectPMID:18931302
synthetic growth defect withcdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic Growth DefectPMID:15743909
synthetic growth defect withnda3tubulin beta Nda3 Synthetic Growth DefectPMID:15743909
synthetic growth defect withhob3BAR adaptor protein Hob3 Synthetic Growth DefectPMID:18931302
synthetic growth defect withain1alpha-actinin Synthetic Growth DefectPMID:15743909
negative genetic interaction withecm29proteasome assembly chaperone Ecm29 Negative GeneticPMID:22681890
negative genetic interaction withSPAC12B10.01cHECT-type ubiquitin-protein ligase E3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC144.05ATP-dependent DNA helicase/ ubiquitin-protein ligase E3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhht1histone H3 h3.1 Negative GeneticPMID:22681890
negative genetic interaction withhhf1histone H4 h4.1 Negative GeneticPMID:22681890
negative genetic interaction withrok1ATP-dependent RNA helicase Rok1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withelp2elongator complex subunit Elp2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpar1protein phosphatase PP2A regulatory subunit B-56 Par1 Negative GeneticPMID:22681890
negative genetic interaction withmid1medial ring protein Mid1 Negative GeneticPMID:22681890
negative genetic interaction withrpb9DNA-directed RNA polymerase II complex subunit Rpb9 Negative GeneticPMID:22681890
negative genetic interaction withpom1DYRK family protein kinase Pom1 Negative GeneticPMID:22681890
negative genetic interaction withcrn1actin binding protein, coronin Crn1 Negative GeneticPMID:22681890
negative genetic interaction withdlc1dynein light chain Dlc1 Negative GeneticPMID:22681890
synthetic lethal withcdc3profilin Synthetic LethalityPMID:16866873
synthetic lethal withadf1actin depolymerizing factor, cofilin Synthetic LethalityPMID:16467379
synthetic lethal withaip1actin cortical patch component Aip1 Synthetic LethalityPMID:25143407
synthetic lethal withfim1fimbrin Synthetic LethalityPMID:15743909
synthetic lethal withcdc4myosin II light chain Synthetic LethalityPMID:16866873
synthetic lethal withact1actin Act1 Synthetic LethalityPMID:15743909
phenotype enhanced byain1alpha-actinin Phenotypic EnhancementPMID:16866873
phenotype enhanced byadf1actin depolymerizing factor, cofilin Phenotypic EnhancementPMID:16866873
phenotype enhanced bycdc4myosin II light chain Phenotypic EnhancementPMID:16866873
phenotype enhanced byrng2IQGAP Phenotypic EnhancementPMID:16866873
phenotype enhanced bycdc3profilin Phenotypic EnhancementPMID:16866873
positive genetic interaction withvps17retromer complex subunit Vps17 Positive GeneticPMID:22681890
positive genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Positive GeneticPMID:22681890
positive genetic interaction withsty1MAP kinase Sty1 Positive GeneticPMID:22681890
positive genetic interaction withset1histone lysine methyltransferase Set1 Positive GeneticPMID:22681890
positive genetic interaction withSPAC1A6.03clysophospholipase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withvps5retromer complex subunit Vps5 Positive GeneticPMID:22681890
positive genetic interaction withppm1leucine carboxyl methyltransferase, involved in regulation of autophagy Ppm1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withurk1uridine kinase/uracil phosphoribosyltransferase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withprz1calcineurin responsive transcription factor Prz1 Positive GeneticPMID:22681890
positive genetic interaction withSPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
synthetically rescued bymyo2myosin II heavy chain Synthetic RescuePMID:15743909
synthetically rescued bycdc12formin Cdc12 Synthetic RescuePMID:15743909
enhances phenotype ofpac10prefoldin subunit 3 Pac10 (predicted) Phenotypic EnhancementPMID:19547744
enhances phenotype ofacp1F-actin capping protein alpha subunit Phenotypic EnhancementPMID:25143407
synthetically rescuescdc12formin Cdc12 Synthetic RescuePMID:15743909
External References
Database Identifier Description
NBRP SPAC631.01c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC631.01c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC631.01c BioGRID Interaction Datasets
Expression Viewer SPAC631.01c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC631.01c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC631.01c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC631.01c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC631.01c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC631.01c Transcriptome Viewer (Bähler Lab)
GEO SPAC631.01c GEO profiles
PInt SPAC631.01c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC631.01c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC631.01c Fission yeast phenotypic data & analysis
Cyclebase SPAC631.01c.1 Cell Cycle Data
SPD / RIKEN32/32D06Orfeome Localization Data
UniProtKB/SwissProtQ9HGP5F-actin-capping protein subunit beta
ModBaseQ9HGP5Database of comparative protein structure models
STRINGQ9HGP5Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593619F-actin capping protein beta subunit (predicted)
RefSeq mRNANM_001019050972h- F-actin capping protein beta subunit (predicted) (acp2), mRNA

Literature for acp2

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016