vma2 (SPAC637.05c)


Gene Standard Namevma2 Characterisation Statuspublished
Systematic IDSPAC637.05c Feature Typeprotein coding
Synonyms Name Description
ProductV-type ATPase V1 subunit B Product Size503aa, 55.84 kDa
Genomic Location Chromosome I, 4548985-4546813 (2173nt); CDS:4548828-4547080 (1749nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAIPR000194GO_REF:0000002559
GO:0046961proton-transporting ATPase activity, rotational mechanismISOSGD:S000000331GO_REF:000002429
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0015991ATP hydrolysis coupled proton transportISOSGD:S000000331GO_REF:000002417
GO:0007035vacuolar acidificationISOSGD:S000000331GO_REF:000002423
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmIDAPMID:168233724198
GO:0000221vacuolar proton-transporting V-type ATPase, V1 domainISOSGD:S000000331GO_REF:00000248
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopyvma2ΔNullPMID:204732891309
Microscopyvma2ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000311inviable after spore germination with normal, unseptated germ tube morphologyMicroscopyvma2ΔNullPECO:0000005, PECO:0000137PMID:23697806237
FYPO:0002151inviable sporeMicroscopyvma2ΔNullPECO:0000005, PECO:0000137PMID:23697806447
penetrance FYPO_EXT:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
145489854548788
245487464548675
345486164548568
445484744547625
545475794546813

UTRs

Region Start End Reference
three_prime_UTR45470794546813PMID:21511999
five_prime_UTR45489854548829PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00306 Pfam IPR000793 ATPase, F1/V1/A1 complex, alpha/beta subunit, C-terminal 395 491 4
PF02874 Pfam IPR004100 ATPase, F1 complex alpha/beta subunit, N-terminal domain 28 93 4
PF00006 Pfam IPR000194 ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain 149 377 4
PS00152 Prosite Patterns IPR020003 ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain 368 377 4
PTHR15184:SF11 HMMPANTHER 4 494 1
PTHR15184 HMMPANTHER 4 494 4
G3DSA:3.40.50.300 Gene3D 96 384 279
SSF52540 SuperFamily IPR027417 97 383 294
TIGR01040 tigrfam IPR005723 ATPase, V1 complex, subunit B 24 487 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.02 Da
Charge -10.50
Isoelectric point 4.98
Molecular weight 55.84 kDa
Number of residues 503
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residuePMID:195477441192
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
45138.16during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
54217.32during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
12during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
deafness3
renal tubular acidosis2
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
slm9hira protein Slm9 Affinity Capture-MSPMID:20976105
External References
Database Identifier Description
NBRP SPAC637.05c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC637.05c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC637.05c BioGRID Interaction Datasets
Expression Viewer SPAC637.05c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC637.05c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC637.05c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC637.05c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC637.05c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC637.05c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC637.05c Cell Cycle Data
GEO SPAC637.05c GEO profiles
PInt SPAC637.05c Protein-Protein Interaction Predictor (Bähler Lab)
SPD / RIKEN33/33D05Orfeome Localization Data
WikiGene2543408V-type ATPase V1 subunit B
EntrezGene2543408V-type ATPase V1 subunit B
IntEnz3.6.3.14Integrated relational Enzyme database
Rhea3.6.3.14Annotated reactions database
UniProtKB/SwissProtP31411V-type proton ATPase subunit B
ModBaseP31411Database of comparative protein structure models
StringP31411Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594623V-type ATPase V1 subunit B
RefSeq mRNANM_001020051972h- V-type ATPase V1 subunit B (vma2), mRNA
European Nucleotide ArchiveCAA22584ENA Protein Mapping
European Nucleotide ArchiveCAA49339ENA Protein Mapping
UniParcUPI0000138168UniProt Archive

Literature for vma2

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014