vma2 (SPAC637.05c)


Gene Standard Namevma2 Characterisation Statuspublished
Systematic IDSPAC637.05c Feature Typeprotein coding
Synonyms Name Description
ProductV-type ATPase V1 subunit B Product Size503aa, 55.84 kDa
Genomic Location Chromosome I, 4548985-4546813 (2173nt); CDS:4548828-4547080 (1749nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding560
Annotation ExtensionEvidenceWith/FromReference
proton-transporting ATPase activity, rotational mechanism29
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
ATP hydrolysis coupled proton transport17
Annotation ExtensionEvidenceWith/FromReference
vacuolar acidification17
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4212
Annotation ExtensionEvidenceWith/FromReference
vacuolar membrane128
Annotation ExtensionEvidenceWith/FromReference
vacuolar proton-transporting V-type ATPase, V1 domain8
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationvma2Δ1452

Cell Phenotype

Term NameGenotypesCount
inviable after spore germination with normal, unseptated germ tube morphologyvma2Δ237
inviable sporevma2Δ476
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4548985..4548788, 4548746..4548675, 4548616..4548568, 4548474..4547625, 4547579..4546813
Intron4548787..4548747, 4548674..4548617, 4548567..4548475, 4547624..4547580
mRNA4548985..4546813
5' UTR4548985..4548829PMID:21511999
CDS4548828..4548788, 4548746..4548675, 4548616..4548568, 4548474..4547625, 4547579..4547080
3' UTR4547079..4546813PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00006 Pfam IPR000194 ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain 150 377 4
PF02874 Pfam IPR004100 ATPase, F1 complex alpha/beta subunit, N-terminal domain 28 93 4
PF00306 Pfam IPR000793 ATPase, F1/V1/A1 complex, alpha/beta subunit, C-terminal 395 491 4
PS00152 Prosite Patterns IPR020003 ATPase, alpha/beta subunit, nucleotide-binding domain, active site 368 377 4
PTHR15184 HMMPANTHER 1 503 4
PTHR15184:SF11 HMMPANTHER 1 503 1
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 96 384 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 97 383 294
PIRSF039114 PIRSF IPR022879 V-type ATP synthase regulatory subunit B/beta 14 492 1
MF_00310 HAMAP IPR022879 V-type ATP synthase regulatory subunit B/beta 21 486 1
TIGR01040 TIGRFAM IPR005723 ATPase, V1 complex, subunit B 24 487 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.02 Da
Charge -10.50
Codon Adaptation Index 0.62
Isoelectric point 4.98
Molecular weight 55.84 kDa
Number of residues 503
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1677
present during mitotic M phaseS502
present during mitotic M phaseS491
present during mitotic M phaseS492
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S502 PMID:21712547
present during mitotic M phase experimental evidence S491 PMID:21712547
present during mitotic M phase experimental evidence S492 PMID:21712547
O-phospho-L-threonine 701
present during mitotic M phaseT489
present during mitotic M phaseT499
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T489 PMID:21712547
present during mitotic M phase experimental evidence T499 PMID:21712547
phosphorylated residue 1932
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
ubiquitinylated lysineK493 512
Annotation ExtensionEvidenceResidueReference
mass spectrometry evidence K493 PMID:26412298
IDA PMID:26412298
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for vma2 (SPAC637.05c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
156304during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
155763during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
136270during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
177935during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
45138.16during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
138592during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
54217.32during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
12during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
deafness3
renal tubular acidosis2
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC637.05c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
affinity captured byslm9hira protein Slm9 Affinity Capture-MSPMID:20976105
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
External References
Database Identifier Description
NBRP SPAC637.05c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC637.05c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC637.05c BioGRID Interaction Datasets
Expression Viewer SPAC637.05c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC637.05c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC637.05c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC637.05c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC637.05c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC637.05c Transcriptome Viewer (Bähler Lab)
GEO SPAC637.05c GEO profiles
PInt SPAC637.05c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC637.05c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC637.05c Fission yeast phenotypic data & analysis
Cyclebase SPAC637.05c.1 Cell Cycle Data
SPD / RIKEN33/33D05Orfeome Localization Data
IntEnz3.6.3.14Integrated relational Enzyme database
Rhea3.6.3.14Annotated reactions database
UniProtKB/SwissProtP31411V-type proton ATPase subunit B
ModBaseP31411Database of comparative protein structure models
STRINGP31411Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594623V-type ATPase V1 subunit B
RefSeq mRNANM_001020051972h- V-type ATPase V1 subunit B (vma2), mRNA
European Nucleotide ArchiveCAA22584.1ENA Protein Mapping
European Nucleotide ArchiveCAA49339.1ENA Protein Mapping
MetaCycPWY-7219Adenosine ribonucleotides de novo biosynthesis
KEGG00190+3.6.3.14Oxidative phosphorylation
UniParcUPI0000138168UniProt Archive

Literature for vma2

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016