cdc5 (SPAC644.12)


Gene Standard Namecdc5 Characterisation Statuspublished
Systematic IDSPAC644.12 Feature Typeprotein coding
Synonyms Name DescriptionCell Division Cycle
Productsplicing factor, Prp19 complex subunit Cdc5 Product Size757aa, 86.84 kDa
Genomic Location Chromosome I, 2694717-2697430 (2714nt); CDS:2694758-2697334 (2577nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0003677DNA bindingIDAPMID:8313892385
GO:0005515protein bindingIPIprp5PMID:12088152865
IPIprp19PMID:12088152
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0007049cell cycleIEAUniProtKB-KW:KW-0131GO_REF:0000037789
GO:0045292mRNA cis splicing, via spliceosomeIMPPMID:10409726112
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000974Prp19 complexIDAPMID:2138689716
IDAPMID:10409726
GO:0005829cytosol2319
during meiotic anaphase IIIDAPMID:20970342
GO:0005634nucleusIDAPMID:104097262737
during meiotic prophase IIIDAPMID:20970342
during meiotic metaphase IIIDAPMID:20970342
during meiosis IIDAPMID:20970342
IDAPMID:16823372
during meiotic telophase IIIDAPMID:20970342
GO:0005681spliceosomal complexIDAPMID:11884590107
IDAPMID:12088152
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationCell growth assaycdc5-120Not specifiedPECO:0000126, PECO:0000004PMID:83138921337
Cell growth assaycdc5-120Not specifiedPECO:0000137, PECO:0000004PMID:8313892
Microscopycdc5ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopycdc5ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopycdc5ΔNullPECO:0000005, PECO:0000137PMID:23697806640
FYPO:0000444abnormal mitotic cell cycle arrest with replicated DNAFlow cytometry datacdc5ΔNullPMID:831389226
Flow cytometry datacdc5-120Not specifiedPECO:0000004PMID:8313892
FYPO:0000141abnormal mitotic sister chromatid segregationMicroscopycdc5ΔNullPMID:8313892141
FYPO:0002044abolished premeiotic DNA replicationFlow cytometry datacdc5-120Not specifiedPECO:0000229, PECO:0000004PMID:108713417
FYPO:0000446cell cycle arrest in mitotic G2 phaseOthercdc5-120Not specifiedPECO:0000004PMID:831389225
FYPO:0003029decreased mRNA splicing, via spliceosome16
affecting taf111Gel electrophoresis evidencecdc5+ (wild type)KnockdownPMID:10409726
FYPO:0003041decreased snRNA splicing14
affecting snu6Gel electrophoresis evidencecdc5+ (wild type)KnockdownPMID:10409726
FYPO:0000316inviable after spore germinationMicroscopycdc5ΔNullPMID:8313892774
FYPO:0000314inviable after spore germination with elongated germ tube163
penetrance FYPO_EXT:0000001Microscopycdc5ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0000839inviable elongated mononucleate aseptate cellMicroscopycdc5-120EndogenousPECO:0000004PMID:95820130
Microscopycdc5ΔNullPMID:8313892
FYPO:0002251inviable swollen elongated vegetative cellMicroscopycdc5-120Not specifiedPECO:0000004PMID:831389224
FYPO:0002888mitotic cell cycle arrest before cell separationMicroscopycdc5ΔNullPMID:83138922
FYPO:0000590normal sporulationCell growth assaycdc5-120PMID:193412645
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in hub1 ubiquitin-like protein modifier Hub1 PMID:15620657
FYPO affected by mutation in smn1 SMN family protein Smn1 PMID:20400941
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
126947172694776
226948172694836
326949032694917
426949822695101
526951712695619
626956842697430
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13921 Pfam 9 69 4
PF11831 Pfam IPR021786 Domain of unknown function DUF3351 414 621 1
SM00717 SMART IPR001005 SANT/Myb domain 57 104 15
SM00717 SMART IPR001005 SANT/Myb domain 5 54 15
PS51294 Prosite Profiles IPR017930 Myb domain 1 56 5
PS51294 Prosite Profiles IPR017930 Myb domain 57 106 5
PTHR10641 HMMPANTHER 5 645 3
PTHR10641:SF17 HMMPANTHER 5 645 1
1.10.10.60 Gene3D IPR009057 Homeodomain-like 56 103 24
1.10.10.60 Gene3D IPR009057 Homeodomain-like 4 55 24
SSF46689 SuperFamily IPR009057 Homeodomain-like 5 49 25
SSF46689 SuperFamily IPR009057 Homeodomain-like 36 108 25
Coil ncoils Rabaptin coiled-coil domain 289 310 968
Coil ncoils Rabaptin coiled-coil domain 91 116 968
Coil ncoils Rabaptin coiled-coil domain 658 679 968

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001260Myb familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001260

Protein Properties

Ave. residue weight 114.72 Da
Charge 5.00
Isoelectric point 8.14
Molecular weight 86.84 kDa
Number of residues 757
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residueNASPMID:195477441922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1369during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1086during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1128during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1400during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1275during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2623.94during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
4297.63during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.8during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.95during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
dre4splicing associated factor Dre4 Synthetic LethalityPMID:21386897
prp5WD repeat protein Prp5 Synthetic Growth DefectPMID:10409726
pam17TIM23 translocase complex-associated motor subunit Pam17 (predicted) Negative GeneticPMID:22681890
hub1ubiquitin-like protein modifier Hub1 Synthetic Growth DefectPMID:15620657
SPCC584.01csulfite reductase NADPH flavoprotein subunit (predicted) Negative GeneticPMID:22681890
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Negative GeneticPMID:8313892
trx2mitochondrial thioredoxin Trx2 Negative GeneticPMID:22681890
cwf10U5 snRNP GTPase subunit Cwf10 Synthetic LethalityPMID:24014766
cwf11complexed with Cdc5 protein Cwf11 Synthetic Growth DefectPMID:11884590
SPAC17H9.08mitochondrial coenzyme A transporter (predicted) Negative GeneticPMID:22681890
ulp2SUMO deconjugating cysteine peptidase Ulp2 (predicted) Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
saf2splicing associated factor Saf2 Affinity Capture-MSPMID:21386897
prp43ATP-dependent RNA helicase Prp43 Co-purificationPMID:24014766
cyp1cyclophilin family peptidyl-prolyl cis-trans isomerase Cyp1 Co-purificationPMID:24014766
smd1Sm snRNP core protein Smd1 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
sum3translation initiation RNA helicase Sum3 Co-purificationPMID:24014766
srp2mRNA export factor Srp2 Co-purificationPMID:24014766
prp45splicing factor Prp45 Affinity Capture-WesternPMID:11884590
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
cwf28splicing factor Cwf28 Affinity Capture-MSPMID:15755920
cwf19complexed with Cdc5 protein Cwf19 Affinity Capture-MSPMID:11884590
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
cwf14G10 protein Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
cwf10U5 snRNP GTPase subunit Cwf10 Affinity Capture-WesternPMID:24442611
Affinity Capture-MS
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-WesternPMID:10409726
Affinity Capture-MS
saf4splicing associated factor Saf4 Co-purificationPMID:24014766
cwf16splicing factor Cwf16 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
saf1splicing associated factor Saf1 Affinity Capture-MSPMID:21386897
lea1U2 snRNP-associated protein Lea1 (predicted) Affinity Capture-MSPMID:24442611
Affinity Capture-Western
Co-purificationPMID:24014766
cwf26complexed with Cdc5 protein Cwf26 Co-purificationPMID:24014766
spf38U5 snRNP complex subunit Spf38 Affinity Capture-MSPMID:11884590
Affinity Capture-Western
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
cwf20complexed with Cdc5 protein Cwf20 Affinity Capture-MSPMID:11884590
Affinity Capture-MSPMID:15755920
cwf21complexed with Cdc5 protein Cwf21 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
snu2small nuclear RNA U2 Affinity Capture-RNAPMID:10409726
dre4splicing associated factor Dre4 Affinity Capture-WesternPMID:21386897
Affinity Capture-MS
snu6small nuclear RNA U6 Affinity Capture-RNAPMID:10409726
prp10U2 snRNP-associated protein Sap155 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
slu7splicing factor Slu7 Affinity Capture-MSPMID:15755920
prp16ATP-dependent RNA helicase Prp16 Co-purificationPMID:24014766
syf2splicing factor Syf2 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
lsm8U6 snRNP-associated protein Lsm8 (predicted) Affinity Capture-WesternPMID:11884590
cwf2RNA-binding protein Cwf2 Affinity Capture-MSPMID:21386897
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-WesternPMID:10409726
Affinity Capture-MS
cwf3complexed with Cdc5 protein Cwf3 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-WesternPMID:10409726
Affinity Capture-MS
smd3Sm snRNP core protein Smd3 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
sap145U2 snRNP-associated protein Sap145 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
prp22ATP-dependent RNA helicase Prp22 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
cwf4complexed with Cdc5 protein Cwf4 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-WesternPMID:10409726
Affinity Capture-MS
cwf25complexed with Cdc5 protein Cwf25 Co-purificationPMID:24014766
nab2poly(A) binding protein Nab2 (predicted) Affinity Capture-MSPMID:24081329
cwf29RNA-binding protein Cwf29 Affinity Capture-MSPMID:15755920
dsk1SR protein-specific kinase Dsk1 Co-purificationPMID:24014766
spp42U5 snRNP complex subunit Spp42 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-MSPMID:10409726
cwf5RNA-binding protein Cwf5 Affinity Capture-WesternPMID:11884590
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-MSPMID:10409726
mug161CwfJ family protein, splicing factor (predicted) Co-purificationPMID:24014766
snu5small nuclear RNA U5 Affinity Capture-RNAPMID:10409726
ini1RING finger-like protein Ini1 Co-purificationPMID:24014766
mei4meiotic forkhead transcription factor Mei4 Affinity Capture-WesternPMID:18815595
cwf7splicing factor Cwf7 Affinity Capture-WesternPMID:11884590
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-MSPMID:10409726
cwf15complexed with Cdc5 protein Cwf15 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
cwf11complexed with Cdc5 protein Cwf11 Affinity Capture-WesternPMID:11884590
Affinity Capture-MS
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
sap61U2 snRNP-associated protein sap61 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
cwf22splicing factor Cwf22 Co-purificationPMID:24014766
cwf12complexed with Cdc5 protein Cwf12 Affinity Capture-WesternPMID:11884590
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
cwf18complexed with Cdc5 protein Cwf18 Affinity Capture-MSPMID:11884590
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
cwf24zf-C3HC4 type (RING finger)/GCN5-related N acetyltransferase fusion protein Affinity Capture-MSPMID:15755920
cdc5splicing factor, Prp19 complex subunit Cdc5 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
smd2Sm snRNP core protein Smd2 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-MSPMID:10409726
bpb1zinc finger splicing factor Bpb1 Co-purificationPMID:24014766
smb1Sm snRNP core protein Smb1 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
sap62U2 snRNP Sap62 Affinity Capture-MSPMID:15755920
prp19ubiquitin-protein ligase E4 Affinity Capture-MSPMID:21386897
Affinity Capture-WesternPMID:20462492
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-MSPMID:10409726
prp12U2 snRNP-associated protein Sap130 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
prp5WD repeat protein Prp5 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-MSPMID:10409726
Affinity Capture-Western
usp104U1 snRNP-associated protein Usp104 Co-purificationPMID:24014766
sme1Sm snRNP core protein Sme1 Co-purificationPMID:24014766
SPAC29A4.06csplicing protein, human NSRP1 ortholog Co-purificationPMID:24014766
cdc28ATP-dependent RNA helicase Cdc28 Co-purificationPMID:24014766
saf3splicing associated factor Saf3 Affinity Capture-MSPMID:21386897
rsd1RNA-binding protein Rsd1 (predicted) Co-purificationPMID:24014766
brr2U5 snRNP complex subunit Brr2 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
prp17splicing factor Prp17 Affinity Capture-MSPMID:21386897
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
smg1Sm snRNP core protein Smg1 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
sap114U2 snRNP subunit Sap114 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
cyp7cyclophilin family peptidyl-prolyl cis-trans isomerase Cyp7 (predicted) Affinity Capture-MSPMID:15755920
smf1Sm snRNP core protein Smf1 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
prp2U2AF large subunit (U2AF-59) Co-purificationPMID:24014766
msl1U2 snRNP-associated protein Msl1 (predicted) Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
usp105U1 snRNP-associated protein Usp105 Co-purificationPMID:24014766
External References
Database Identifier Description
NBRP SPAC644.12 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC644.12 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC644.12 BioGRID Interaction Datasets
Expression Viewer SPAC644.12 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC644.12 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC644.12 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC644.12 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC644.12 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC644.12 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC644.12 Cell Cycle Data
GEO SPAC644.12 GEO profiles
PInt SPAC644.12 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC644.12 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN38/38E01Orfeome Localization Data
UniProtKB/SwissProtP39964Pre-mRNA-splicing factor cdc5
ModBaseP39964Database of comparative protein structure models
STRINGP39964Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593880cell division control protein, splicing factor Cdc5
RefSeq mRNANM_001019310972h- cell division control protein, splicing factor Cdc5 (cdc5), mRNA
European Nucleotide ArchiveL19525ENA EMBL mapping
European Nucleotide ArchiveCAB90139.1ENA Protein Mapping
UniParcUPI0000127257UniProt Archive

Literature for cdc5

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014