cdc5 (SPAC644.12)


Gene Standard Namecdc5 Characterisation Statuspublished
Systematic IDSPAC644.12 Feature Typeprotein coding
Synonyms Name DescriptionCell Division Cycle
Productsplicing factor, Prp19 complex subunit Cdc5 Product Size757aa, 86.84 kDa
Genomic Location Chromosome I, 2694717-2697430 (2714nt); CDS:2694758-2697334 (2577nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
DNA binding381
Annotation ExtensionEvidenceWith/FromReference
protein binding848
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mRNA cis splicing, via spliceosome118
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationcdc5-120Not specified1427
cdc5ΔNull

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic cell cyclecdc5ΔNull794
abnormal mitotic cell cycle arrest with replicated DNAcdc5-120Not specified32
cdc5ΔNull
abnormal mitotic sister chromatid segregationcdc5ΔNull179
abolished premeiotic DNA replicationcdc5-120Not specified14
cell cycle arrest in mitotic G2 phasecdc5-120Not specified26
decreased mRNA splicing, via spliceosome32
affecting taf111cdc5+ (wild type)Knockdown
decreased snRNA splicing14
affecting snu6cdc5+ (wild type)Knockdown
inviable after spore germinationcdc5ΔNull793
inviable after spore germination with elongated germ tube163
penetrance FYPO_EXT:0000001cdc5ΔNull
inviable elongated mononucleate aseptate cellcdc5-120Endogenous37
cdc5ΔNull
inviable swollen elongated vegetative cellcdc5-120Not specified25
mitotic cell cycle arrest before cell separationcdc5ΔNull7
normal sporulationcdc5-12049
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in iss10 Mmi1-driven selective elimination factor Iss10 PMID:24713849
FYPO affected by mutation in mtl1 TRAMP complex ATP-dependent RNA helicase (predicted) PMID:24713849
FYPO affected by mutation in rmn1 RNA-binding protein PMID:24713849
FYPO affected by mutation in smn1 SMN family protein Smn1 PMID:20400941
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
126947172694776
226948172694836
326949032694917
426949822695101
526951712695619
626956842697430
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF11831 Pfam IPR021786 Domain of unknown function DUF3351 414 621 1
PF13921 Pfam 9 69 4
SM00717 SMART IPR001005 SANT/Myb domain 57 104 15
SM00717 SMART IPR001005 SANT/Myb domain 5 54 15
PS51294 Prosite Profiles IPR017930 Myb domain 1 56 5
PS51294 Prosite Profiles IPR017930 Myb domain 57 106 5
PTHR10641 HMMPANTHER 2 638 3
PTHR10641:SF17 HMMPANTHER 2 638 1
1.10.10.60 Gene3D IPR009057 Homeodomain-like 56 103 24
1.10.10.60 Gene3D IPR009057 Homeodomain-like 4 55 24
SSF46689 SuperFamily IPR009057 Homeodomain-like 36 108 25
SSF46689 SuperFamily IPR009057 Homeodomain-like 5 49 25
Coil ncoils Rabaptin coiled-coil domain 289 310 971
Coil ncoils Rabaptin coiled-coil domain 91 116 971
Coil ncoils Rabaptin coiled-coil domain 658 679 971

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001260Myb familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001260

Protein Properties

Ave. residue weight 114.72 Da
Charge 5.00
Isoelectric point 8.14
Molecular weight 86.84 kDa
Number of residues 757
Modifications

Protein Modifications

Term NameResidueCount
phosphorylated residue 1922
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1369during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1086during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1128during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1400during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2623.94during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1275during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
4297.63during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.8during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.95during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
saf2splicing associated factor Saf2 Affinity Capture-MSPMID:21386897
prp43ATP-dependent RNA helicase Prp43 Co-purificationPMID:24014766
cyp1cyclophilin family peptidyl-prolyl cis-trans isomerase Cyp1 Co-purificationPMID:24014766
smd1Sm snRNP core protein Smd1 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
sum3translation initiation RNA helicase Sum3 Co-purificationPMID:24014766
srp2mRNA export factor Srp2 Co-purificationPMID:24014766
prp45splicing factor Prp45 Affinity Capture-WesternPMID:11884590
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
cwf28splicing factor Cwf28 Affinity Capture-MSPMID:15755920
cwf19complexed with Cdc5 protein Cwf19 Affinity Capture-MSPMID:11884590
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
cwf14G10 protein Affinity Capture-MSPMID:24874881
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
cwf10U5 snRNP GTPase subunit Cwf10 Affinity Capture-MSPMID:24442611
Affinity Capture-Western
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-MSPMID:10409726
Affinity Capture-Western
saf4splicing associated factor Saf4 Co-purificationPMID:24014766
cwf16splicing factor Cwf16 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
saf1splicing associated factor Saf1 Affinity Capture-MSPMID:21386897
lea1U2 snRNP-associated protein Lea1 (predicted) Affinity Capture-MSPMID:24442611
Affinity Capture-Western
Co-purificationPMID:24014766
cwf26complexed with Cdc5 protein Cwf26 Co-purificationPMID:24014766
spf38U5 snRNP complex subunit Spf38 Affinity Capture-MSPMID:11884590
Affinity Capture-Western
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
cwf20complexed with Cdc5 protein Cwf20 Affinity Capture-MSPMID:11884590
Affinity Capture-MSPMID:15755920
cwf21complexed with Cdc5 protein Cwf21 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
snu2small nuclear RNA U2 Protein-RNAPMID:25263959
Affinity Capture-RNAPMID:10409726
dre4splicing associated factor Dre4 Affinity Capture-MSPMID:21386897
Affinity Capture-Western
snu6small nuclear RNA U6 Protein-RNAPMID:25263959
Affinity Capture-RNAPMID:10409726
pir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
prp10U2 snRNP-associated protein Sap155 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
slu7splicing factor Slu7 Affinity Capture-MSPMID:15755920
prp16ATP-dependent RNA helicase Prp16 Co-purificationPMID:24014766
syf2splicing factor Syf2 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
lsm8U6 snRNP-associated protein Lsm8 (predicted) Affinity Capture-WesternPMID:11884590
cwf2RNA-binding protein Cwf2 Affinity Capture-MSPMID:21386897
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-WesternPMID:10409726
Affinity Capture-MS
cwf3complexed with Cdc5 protein Cwf3 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-MSPMID:10409726
Affinity Capture-Western
smd3Sm snRNP core protein Smd3 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
sap145U2 snRNP-associated protein Sap145 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
mtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
prp22ATP-dependent RNA helicase Prp22 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
cwf4complexed with Cdc5 protein Cwf4 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-WesternPMID:10409726
Affinity Capture-MS
cwf25complexed with Cdc5 protein Cwf25 Co-purificationPMID:24014766
nab2poly(A) binding protein Nab2 (predicted) Affinity Capture-MSPMID:24081329
cwf29RNA-binding protein Cwf29 Affinity Capture-MSPMID:15755920
dsk1SR protein-specific kinase Dsk1 Co-purificationPMID:24014766
spp42U5 snRNP complex subunit Spp42 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-MSPMID:10409726
cwf5RNA-binding protein Cwf5 Affinity Capture-WesternPMID:11884590
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-MSPMID:10409726
mug161CwfJ family protein, splicing factor (predicted) Co-purificationPMID:24014766
snu5small nuclear RNA U5 Affinity Capture-RNAPMID:10409726
ini1RING finger-like protein Ini1 Co-purificationPMID:24014766
mei4meiotic forkhead transcription factor Mei4 Affinity Capture-WesternPMID:18815595
cwf7splicing factor Cwf7 Affinity Capture-WesternPMID:11884590
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-MSPMID:10409726
cwf15complexed with Cdc5 protein Cwf15 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
cwf11complexed with Cdc5 protein Cwf11 Affinity Capture-WesternPMID:11884590
Affinity Capture-MS
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
sap61U2 snRNP-associated protein sap61 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
cwf22splicing factor Cwf22 Co-purificationPMID:24014766
cwf12complexed with Cdc5 protein Cwf12 Affinity Capture-WesternPMID:11884590
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
cwf18complexed with Cdc5 protein Cwf18 Affinity Capture-MSPMID:11884590
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
cwf24ubiquitin-protein ligase E3/GCN5-related N acetyltransferase fusion protein Affinity Capture-MSPMID:15755920
cdc5splicing factor, Prp19 complex subunit Cdc5 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
red1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
smd2Sm snRNP core protein Smd2 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-MSPMID:10409726
bpb1zinc finger splicing factor Bpb1 Co-purificationPMID:24014766
smb1Sm snRNP core protein Smb1 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
sap62U2 snRNP Sap62 Affinity Capture-MSPMID:15755920
prp19ubiquitin-protein ligase E4 Affinity Capture-MSPMID:21386897
Affinity Capture-WesternPMID:20462492
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-MSPMID:10409726
prp12U2 snRNP-associated protein Sap130 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
prp5WD repeat protein Prp5 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
Affinity Capture-MSPMID:10409726
Affinity Capture-Western
usp104U1 snRNP-associated protein Usp104 Co-purificationPMID:24014766
saf5splicing factor Affinity Capture-MSPMID:25274039
SPAC29A4.06csplicing protein, human NSRP1 ortholog Co-purificationPMID:24014766
cdc28ATP-dependent RNA helicase Cdc28 Co-purificationPMID:24014766
saf3splicing associated factor Saf3 Affinity Capture-MSPMID:21386897
rsd1RNA-binding protein Rsd1 (predicted) Co-purificationPMID:24014766
brr2U5 snRNP complex subunit Brr2 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
prp17splicing factor Prp17 Affinity Capture-MSPMID:21386897
Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
smg1Sm snRNP core protein Smg1 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
sap114U2 snRNP subunit Sap114 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
sde2silencing defective protein Sde2 Affinity Capture-MSPMID:25274039
cyp7cyclophilin family peptidyl-prolyl cis-trans isomerase Cyp7 (predicted) Affinity Capture-MSPMID:15755920
sme1Sm snRNP core protein Sme1 Co-purificationPMID:24014766
smf1Sm snRNP core protein Smf1 Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
prp2U2AF large subunit (U2AF-59) Co-purificationPMID:24014766
msl1U2 snRNP-associated protein Msl1 (predicted) Co-purificationPMID:24014766
Affinity Capture-MSPMID:15755920
usp105U1 snRNP-associated protein Usp105 Co-purificationPMID:24014766
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
dre4splicing associated factor Dre4 Synthetic LethalityPMID:21386897
prp5WD repeat protein Prp5 Synthetic Growth DefectPMID:10409726
prp3U4/U6 x U5 tri-snRNP complex subunit Prp3 Synthetic Growth DefectPMID:25263959
pam17TIM23 translocase complex-associated motor subunit Pam17 (predicted) Negative GeneticPMID:22681890
hub1ubiquitin-like protein modifier Hub1 Synthetic Growth DefectPMID:15620657
SPCC584.01csulfite reductase NADPH flavoprotein subunit (predicted) Negative GeneticPMID:22681890
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Negative GeneticPMID:8313892
trx2mitochondrial thioredoxin Trx2 Negative GeneticPMID:22681890
cwf10U5 snRNP GTPase subunit Cwf10 Synthetic LethalityPMID:24014766
cwf11complexed with Cdc5 protein Cwf11 Synthetic Growth DefectPMID:11884590
SPAC17H9.08mitochondrial coenzyme A transmembrane transporter (predicted) Negative GeneticPMID:22681890
ulp2SUMO deconjugating cysteine peptidase Ulp2 (predicted) Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC644.12 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC644.12 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC644.12 BioGRID Interaction Datasets
Expression Viewer SPAC644.12 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC644.12 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC644.12 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC644.12 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC644.12 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC644.12 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC644.12 Cell Cycle Data
GEO SPAC644.12 GEO profiles
PInt SPAC644.12 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC644.12 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC644.12 Fission yeast phenotypic data & analysis
SPD / RIKEN38/38E01Orfeome Localization Data
UniProtKB/SwissProtP39964Pre-mRNA-splicing factor cdc5
ModBaseP39964Database of comparative protein structure models
STRINGP39964Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593880cell division control protein, splicing factor Cdc5
RefSeq mRNANM_001019310972h- cell division control protein, splicing factor Cdc5 (cdc5), mRNA
European Nucleotide ArchiveL19525ENA EMBL mapping
European Nucleotide ArchiveCAB90139.1ENA Protein Mapping
UniParcUPI0000127257UniProt Archive

Literature for cdc5

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015