set13 (SPAC688.14)


Gene Standard Nameset13 Characterisation Statuspublished
Systematic IDSPAC688.14 Feature Typeprotein coding
Synonyms Name Description
Productribosome L32 lysine methyltransferase Set13 Product Size468aa, 54.02 kDa
Genomic Location Chromosome I, 3141223-3143183 (1961nt); CDS:3141463-3143034 (1572nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0016279protein-lysine N-methyltransferase activity14
has substrate rpl42IDAPMID:20444689
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0018022peptidyl-lysine methylationIDAPMID:2044468912
GO:0006479protein methylationIDAPMID:2044468930
GO:2000765regulation of cytoplasmic translationNASGO_REF:000000118
GO:0043555regulation of translation in response to stressIMPPMID:204446899
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005634nucleusIDAPMID:168233722737
IDAPMID:20444689
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000088sensitive to hydroxyureaCell growth assayset13ΔNullPECO:0000137, PECO:0000005PMID:23173672513
FYPO:0000089sensitive to methyl methanesulfonateCell growth assayset13ΔNullPECO:0000137, PECO:0000005PMID:23173672214
FYPO:0002060viable vegetative cell populationMicroscopyset13ΔNullPMID:204732893751
Microscopyset13ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopyset13ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
131412233141603
231416523142329
331423963142667
431427193143183
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00856 Pfam IPR001214 SET domain 37 302 12
PS50280 Prosite Profiles IPR001214 SET domain 22 302 13
PTHR13271 HMMPANTHER 1 467 5
2h21C01 Gene3D 4 185 5
2h21C01 Gene3D 255 316 5
SSF82199 SuperFamily 4 187 13
SSF82199 SuperFamily 257 320 13
PIRSF011771 PIRSF IPR011383 RuBisCO-cytochrome methylase, RMS1 1 468 1

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001327SET domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001327

Protein Properties

Ave. residue weight 115.43 Da
Charge -19.50
Isoelectric point 4.68
Molecular weight 54.02 kDa
Number of residues 468
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS200PMID:217125471670
present during mitotic M phase
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4333during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3776during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3142during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3598during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
NDduring GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
3572during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.3during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.35during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999432
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
fin1serine/threonine protein kinase, NIMA related Fin1 Positive GeneticPMID:22681890
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
raf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Positive GeneticPMID:18818364
ppk9serine/threonine protein kinase Ppk9 (predicted) Positive GeneticPMID:22681890
pof4elongin-A, F-box protein Pof4 (predicted) Positive GeneticPMID:18818364
SPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:18818364
SPAC19A8.11crecombination protein Irc6 (predicted) Negative GeneticPMID:22681890
eso1sister chromatid cohesion protein/DNA polymerase eta Eso1 fusion protein Negative GeneticPMID:23050226
rxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
sfp1transcription factor Sfp1 (predicted) Negative GeneticPMID:22681890
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:18818364
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
rpl4260S ribosomal protein L36/L42 Biochemical ActivityPMID:20444689
External References
Database Identifier Description
NBRP SPAC688.14 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC688.14 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC688.14 BioGRID Interaction Datasets
Expression Viewer SPAC688.14 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC688.14 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC688.14 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC688.14 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC688.14 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC688.14 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC688.14 Cell Cycle Data
GEO SPAC688.14 GEO profiles
PInt SPAC688.14 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC688.14 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN25/25B03Orfeome Localization Data
UniProtKB/SwissProtQ9P6L2Ribosomal N-lysine methyltransferase 4
ModBaseQ9P6L2Database of comparative protein structure models
STRINGQ9P6L2Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594072ribosome L32 lysine methyltransferase Set13
RefSeq mRNANM_001019496972h- ribosome L32 lysine methyltransferase Set13 (set13), mRNA
European Nucleotide ArchiveCAB90780.2ENA Protein Mapping
UniParcUPI000228F416UniProt Archive

Literature for set13

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014