mrc1 (SPAC694.06c)

Gene Standard Namemrc1 ChromosomeI
Systematic IDSPAC694.06c Gene Start4212453
Synonyms Gene End4209203
Productmediator of replication checkpoint 1 Gene Length3251
Feature Typeprotein coding CDS Start4212343
Name DescriptionMediator of Replication Checkpoint CDS End4209284
Characterisation Statuspublished CDS Length3060

Ensembl Gene Location
  1. SPAC694.02
  2. SPNCRNA.971
  3. SPAC694.03
  4. SPAC694.04c
  5. rps2502
  6. SPNCRNA.972
  7. mrc1
  8. spt6
  9. has1
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000007abnormal S-phase DNA damage checkpointmrc1delta (deletion)deletionPMID:19037101
FYPO:0000085sensitive to camptothecincompetitive growth assay evidencemrc1delta (deletion)deletionPMID:20537132
FYPO:0000088sensitive to hydroxyureacompetitive growth assay evidencemrc1delta (deletion)deletionPMID:20537132
FYPO:0000266sensitive to DNA damaging agentscompetitive growth assay evidencemrc1delta (deletion)deletionPMID:20537132
competitive growth assay evidencemrc1delta (deletion)deletionPMID:20537132
FYPO:0001491viable vegetative cellMicroscopymrc1delta (deletion)deletionPMID:20473289
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0000403Y-form DNA bindingIDAPMID:15471884
GO:0003677DNA bindingIDAPMID:15471884
GO:0005515protein bindingIPItel1PMID:14585996
IPIrad3PMID:14585996
gene_product_form_id PR:000027613IPIcds1PMID:14585996
GO:0043539protein serine/threonine kinase activator activityTASPMID:15471884
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0006260DNA replicationIEAUniProtKB:KW-0235GO_REF:0000037
GO:0007165signal transductionNASGO_REF:0000051
GO:0007346regulation of mitotic cell cycleNASGO_REF:0000051
GO:0031573intra-S DNA damage checkpointIDAPMID:19037101
GO:0033314mitotic DNA replication checkpoint
activatesIMPPMID:11715016
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0000790nuclear chromatin
gene_product_form_id PR:000027610IDAPMID:14585996
GO:0005634nucleusIDAPMID:16823372
GO:0043234protein complexNASGO_REF:0000051
GO:0043596nuclear replication forkTASPMID:16263721
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
142124534209203

References

Region Start End Reference
three_prime_UTR42092834209203PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF09444 IPR018564 Pfam DNA replication checkpoint mediator, MRC1 domain 699 837 1
Coil ncoils Rabaptin coiled-coil domain 655 676 974
Coil ncoils Rabaptin coiled-coil domain 142 167 974

View domain organization at Pfam

Protein Modifications (PSI-MOD)

Term IDTerm NameEvidenceResidueReference
MOD:00696phosphorylated residueresidueT645PMID:14585996

Manually Curated Family or Sub-families

Term IDTerm NameReferenceAll Genes
PBO:0000453ankyrin repeat proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000453

Protein Properties

Ave. residue weight 112.19 Da
Charge -64.50
Isoelectric point 4.41
Molecular weight 114.32 kDa
Number of residues 1019
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide591.14PMID:23101633
experimental evidencevegetative growth of a single-celled organismPECO:0000005,
PECO:0000014		population_widePMID:23101633
RNA levelexperimental evidencevegetative growth of a single-celled organismPECO:0000014,
PECO:0000005		population_wide1.9PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide0.56PMID:23101633
Species Distribution
DescriptionQualifierReference
predominantly single copy (one to one)
conserved in fungi
conserved in eukaryotes
conserved in metazoa
conserved in vertebrates
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
csn1COP9/signalosome complex subunit Csn1 Synthetic Growth DefectPMID:18931302
srs2ATP-dependent DNA helicase, UvrD subfamily Synthetic Growth DefectPMID:18931302
ypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:18818364
SPBP8B7.23ubiquitin-protein ligase E3 implicated in transcription (predicted) Negative GeneticPMID:18818364
SPBC26H8.05cserine/threonine protein phosphatase (predicted) Negative GeneticPMID:18818364
clr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:18818364
rad17RFC related checkpoint protein Rad17 Negative GeneticPMID:18818364
erf2palmitoyltransferase Erf2 (predicted) Negative GeneticPMID:22681890
atb2tubulin alpha 2 Negative GeneticPMID:18818364
cdc45DNA replication pre-initiation complex subunit Cdc45 Synthetic RescuePMID:16849602
SPBC2A9.03WD40/YVTN repeat-like Negative GeneticPMID:18818364
dcc1Ctf18 RFC-like complex subunit Dcc1 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
myo51myosin type V Negative GeneticPMID:18818364
hhf2histone H4 h4.2 Synthetic RescuePMID:22095079
rap1telomere binding protein Rap1 Negative GeneticPMID:18818364
hst4Sir2 family histone deacetylase Hst4 Synthetic LethalityPMID:18344406
omh6alpha-1,2-mannosyltransferase Omh6 (predicted) Negative GeneticPMID:22681890
hip1hira protein, histone chaperone Hip1 Negative GeneticPMID:18818364
rpl280160S ribosomal protein L27/L28 Negative GeneticPMID:22681890
mug154conserved fungal protein Negative GeneticPMID:18818364
mcl1DNA polymerase alpha accessory factor Mcl1 Negative GeneticPMID:18818364
clr3histone deacetylase (class II) Clr3 Negative GeneticPMID:18818364
cdc20DNA polymerase epsilon catalytic subunit Pol2 Phenotypic EnhancementPMID:18667534
pim1Ran GDP/GTP exchange factor Synthetic LethalityPMID:22927644
ago1argonaute Negative GeneticPMID:18818364
ssb1DNA replication factor A subunit Ssb1 Negative GeneticPMID:18818364
spc34DASH complex subunit Spc34 Negative GeneticPMID:18818364
fep1iron-sensing transcription factor Fep1 Negative GeneticPMID:22681890
hta1histone H2A alpha Synthetic Growth DefectPMID:15226425
vrp1verprolin Negative GeneticPMID:18818364
san1sir antagonist Negative GeneticPMID:18818364
pof3F-box protein Pof3 Synthetic Growth DefectPMID:18931302
ask1DASH complex subunit Ask1 Negative GeneticPMID:18818364
snf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:18818364
bdf1Swr1 complex bromodomain subunit Brf1 Synthetic RescuePMID:22095079
Synthetic Growth Defect
Phenotypic Suppression
dad2DASH complex subunit Dad2 Negative GeneticPMID:18818364
ubc6ubiquitin conjugating enzyme Ubc6 (predicted) Positive GeneticPMID:18818364
lub1WD repeat protein Lub1 Negative GeneticPMID:18818364
sir2Sir2 family histone deacetylase Sir2 Negative GeneticPMID:18818364
SPBC1703.11optic atrophy 3 family protein Negative GeneticPMID:18818364
syj1inositol-polyphosphate 5-phosphatase (synaptojanin homolog 1) Negative GeneticPMID:18818364
ers1RNA-silencing factor Ers1 Negative GeneticPMID:18818364
chp1chromodomain protein Chp1 Negative GeneticPMID:18818364
SPBC36B7.08cnucleosome assembly protein (predicted) Negative GeneticPMID:18818364
rad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Phenotypic SuppressionPMID:15173168
Synthetic Rescue
pob3FACT complex subunit Pob3 Negative GeneticPMID:18818364
mad2spindle checkpoint protein Mad2 Negative GeneticPMID:18818364
SPBP4H10.17ccarboxyl methyl esterase (predicted) Negative GeneticPMID:18818364
rpl160360S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:18818364
SPBC839.03cneddylation protein Dcn1 (predicted) Negative GeneticPMID:18818364
vid21NuA4 histone acetyltransferase complex subunit Vid21 Synthetic RescuePMID:22095079
ctf18RFC-like complex subunit Ctf18 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
SPBC13E7.08cRNA polymerase II associated Paf1 complex (predicted) Negative GeneticPMID:18818364
rpa12DNA-directed RNA polymerase complex I subunit Rpa12 Negative GeneticPMID:18818364
spo20sec14 cytosolic factor family Sec14 Negative GeneticPMID:22681890
swi3replication fork protection complex subunit Swi3 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
mak10NatC N-acetyltransferase complex subunit Mak10 (predicted) Synthetic Growth DefectPMID:18931302
rid1GTPase binding protein Rid1 (predicted) Synthetic Growth DefectPMID:20799962
tas3RITS complex subunit 3 Negative GeneticPMID:18818364
raf2Rik1-associated factor Raf2 Negative GeneticPMID:18818364
erf4palmitoyltransferase complex subunit Erf4 (predicted) Negative GeneticPMID:22681890
orc1origin recognition complex subunit Orc1 Phenotypic EnhancementPMID:18667534
Synthetic Growth DefectPMID:16849602
pho24-nitrophenylphosphatase Negative GeneticPMID:18818364
hrp3ATP-dependent DNA helicase Hrp3 Negative GeneticPMID:18818364
ptn1phosphatidylinositol-3,4,5-trisphosphate3-phosphatase Ptn1 Negative GeneticPMID:18818364
hop1linear element associated protein Hop1 Negative GeneticPMID:18818364
hus1checkpoint clamp complex protein Hus1 Negative GeneticPMID:18818364
pdp1PWWP domain protein Pdp1 Negative GeneticPMID:18818364
mlh1MutL family protein Mlh1 (predicted) Negative GeneticPMID:18818364
rif1telomere length regulator protein Rif1 Phenotypic EnhancementPMID:22279046
cph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:18818364
fft3fun thirty related protein Fft3 Negative GeneticPMID:18818364
mst1KAT5 family histone acetyltransferase Mst1 Synthetic RescuePMID:22095079
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:18818364
hht3histone H3 h3.3 Negative GeneticPMID:18818364
hrd1ubiquitin-protein ligase Hrd1, synviolin family Negative GeneticPMID:18818364
nto1histone acetyltransferase complex subunit Nto1 (predicted) Negative GeneticPMID:18818364
mcm2MCM complex subunit Mcm2 Synthetic RescuePMID:16849602
set9histone lysine methyltransferase Set9 Synthetic Growth DefectPMID:15550243
iqw1WD repeat protein, Iqw1 Negative GeneticPMID:18818364
dfp1Hsk1-Dfp1 kinase complex regulatory subunit Dfp1 Synthetic RescuePMID:22024164
rsv2transcription factor Rsv2 Negative GeneticPMID:18818364
chk1Chk1 protein kinase Synthetic Growth DefectPMID:11715017
top1DNA topoisomerase I Negative GeneticPMID:18818364
trm10tRNA m(1)G methyltransferase Trm10 (predicted) Negative GeneticPMID:18818364
SPBC31F10.12RNA-binding protein Tma20 (predicted) Negative GeneticPMID:18818364
klp6kinesin-like protein Klp6 Negative GeneticPMID:18818364
SPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:18818364
SPBC11C11.11cATP helicase Irc3 (predicted) Negative GeneticPMID:18818364
nse6Smc5-6 complex non-SMC subunit Nse6 Synthetic Growth DefectPMID:18931302
mcm5MCM complex subunit Mcm5 Synthetic RescuePMID:16849602
raf1Rik1-associated factor Raf1 Negative GeneticPMID:18818364
hta2histone H2A beta Synthetic Growth DefectPMID:15226425
mre11Mre11 nuclease Negative GeneticPMID:18818364
tor1phosphatidylinositol kinase Tor1 Synthetic Growth DefectPMID:19546237
SPBC4B4.04translation initiation factor eIF2A (predicted) Negative GeneticPMID:18818364
nrc1bromodomain protein (predicted) Synthetic Growth DefectPMID:22095079
Synthetic Rescue
Phenotypic Suppression
mcm4MCM complex subunit Mcm4/Cdc21 Phenotypic EnhancementPMID:18667534
SPBP8B7.10cU3 snoRNP-associated protein Utp16 (predicted) Negative GeneticPMID:18818364
orc2origin recognition complex subunit Orc2 Phenotypic EnhancementPMID:18667534
SPBC11C11.10tRNA pseudouridine synthase (predicted) Negative GeneticPMID:18818364
slm9hira protein Slm9 Synthetic Growth DefectPMID:18931302
crb2DNA repair protein Rad9 homolog, Rhp9 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
cds1replication checkpoint kinase Cds1 Dosage RescuePMID:15226425
Synthetic RescuePMID:15173168
Phenotypic Suppression
Dosage RescuePMID:11715017
arp8actin-like protein, Ino80 complex subunit Arp8 Negative GeneticPMID:18818364
ddb1damaged DNA binding protein Ddb1 Synthetic Growth DefectPMID:18931302
rik1silencing protein Rik1 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
dcr1dicer Negative GeneticPMID:18818364
hsk1Dbf4(Dfp1)-dependent protein kinase Hsk1 Phenotypic SuppressionPMID:22024164
Synthetic Rescue
Synthetic RescuePMID:22279046
Synthetic RescuePMID:20176980
yaf9YEATS family histone acetyltransferase subunit Yaf9 Negative GeneticPMID:18818364
swi6HP1 family chromodomain protein Swi6 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
msh6MutS protein homolog Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
nse5Smc5-6 complex non-SMC subunit Nse5 Synthetic Growth DefectPMID:18931302
hip4histone promoter control protein Hip4 Negative GeneticPMID:18818364
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hsk1Dbf4(Dfp1)-dependent protein kinase Hsk1 Biochemical ActivityPMID:21099360
swi1replication fork protection complex subunit Swi1 Reconstituted ComplexPMID:20924116
cds1replication checkpoint kinase Cds1 Reconstituted ComplexPMID:16618806
Two-hybridPMID:14585996
Two-hybridPMID:15173168
Two-hybridPMID:11715017
rad3ATR checkpoint kinase Rad3 Biochemical ActivityPMID:21099360
Biochemical ActivityPMID:14585996
Co-localization
tel1ATM checkpoint kinase Biochemical ActivityPMID:14585996
Co-localization
swi3replication fork protection complex subunit Swi3 Reconstituted ComplexPMID:20924116
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External References
Database Identifier Description
NBRP SPAC694.06c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC694.06c Retrieval of eukaryotic orthologs
BioGrid SPAC694.06c BioGRID Interaction Datasets
Bähler Lab SPAC694.06c Cell Cycle Expression Profile
Bähler Lab SPAC694.06c Meiosis/Sporulation Expression Profies
Bähler Lab SPAC694.06c Pheromone response/mating expression profiles
Bähler Lab SPAC694.06c Environmental stress expression profiles
Bähler Lab SPAC694.06c Bähler Lab Transcriptome Viewer
Cyclebase SPAC694.06c Cell Cycle Data
PInt SPAC694.06c Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPAC694.06c Entrez Gene
SPD / RIKEN47/47G10Orfeome Localization Data
WikiGene2543240mediator of replication checkpoint 1
Uniprot_genenameMRC1
EntrezGene2543240mediator of replication checkpoint 1
UniProtKB/SwissProtQ9P7T4Mediator of replication checkpoint protein 1
ModBaseQ9P7T4Database of comparative protein structure models
Pfam Protein DomainsQ9P7T4Pfam Domain Arrangement
RefSeq PeptideNP_594486mediator of replication checkpoint 1
RefSeq mRNANM_001019915972h- mediator of replication checkpoint 1 (mrc1), mRNA
European Nucleotide ArchiveCAB71844ENA Protein Mapping
SPD / RIKEN47/47G10Orfeome Localization Data

Literature for mrc1
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Search: UK PMC or PubMed

Release Version: PomBase:18.34 - 04 Apr 2013