spp1 (SPAC6B12.10c)


Gene Standard Namespp1 Characterisation Statuspublished
Systematic IDSPAC6B12.10c Feature Typeprotein coding
Synonymspri1 Name Description
ProductDNA primase catalytic subunit Spp1 Product Size454aa, 52.01 kDa
Genomic Location Chromosome I, 2430742-2428976 (1767nt); CDS:2430617-2429169 (1449nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
DNA primase activity4
Annotation ExtensionEvidenceWith/FromReference
metal ion binding746
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
synthesis of RNA primer involved in mitotic DNA replication9
Annotation ExtensionEvidenceWith/FromReference
telomere maintenance46
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
alpha DNA polymerase:primase complex5
Annotation ExtensionEvidenceWith/FromReference
nuclear chromatin307
Annotation ExtensionEvidenceWith/FromReference
nucleus2697
Annotation ExtensionEvidenceWith/FromReference
primosome complex2
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationspp1ΔNull1428

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic cell cyclespp1ΔNull808
cutunnamed (unrecorded)94
elongated vegetative cellunnamed (unrecorded)705
inviable after spore germination with elongated germ tubespp1ΔNull163
inviable after spore germination, single or double cell divisionspp1ΔNull101
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
124307422430544
224304592428976

UTRs

Region Coordinates Reference
five_prime_UTR2430742..2430618PMID:21511999
three_prime_UTR2429168..2428976PMID:21511999
exon2430617..2430544,2430459..2429169
intron2430543..2430460
mRNA2430742..2428976
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01896 Pfam IPR002755 DNA primase, small subunit 130 363 1
PTHR10536 HMMPANTHER IPR014052 DNA primase, small subunit, eukaryotic/archaeal 5 454 1
1v33A01 Gene3D 30 212 1
1v33A01 Gene3D 332 390 1
SSF56747 SuperFamily 33 388 1
TIGR00335 tigrfam IPR014052 DNA primase, small subunit, eukaryotic/archaeal 36 383 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.56 Da
Charge 3.50
Isoelectric point 6.91
Molecular weight 52.01 kDa
Number of residues 454
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4129during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3734during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
4074during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
4042during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3820during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
701.69during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.2during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.63during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in bacteria1000
conserved in metazoa3432
conserved in vertebrates3407
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bypir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
affinity captured bypol1DNA polymerase alpha catalytic subunit Affinity Capture-WesternPMID:11027257
affinity captured byspb70DNA polymerase alpha B-subunit Affinity Capture-WesternPMID:15314153
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
rescued by overexpression ofalp14TOG/XMAP14 family protein Alp14 Dosage RescuePMID:15507118
phenotype enhanced bychk1Chk1 protein kinase Phenotypic EnhancementPMID:11160827
synthetically rescuescrb2DNA repair protein Rad9 homolog, Rhp9 Synthetic RescuePMID:12589755
External References
Database Identifier Description
NBRP SPAC6B12.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC6B12.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC6B12.10c BioGRID Interaction Datasets
Expression Viewer SPAC6B12.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC6B12.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC6B12.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC6B12.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC6B12.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC6B12.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC6B12.10c Cell Cycle Data
GEO SPAC6B12.10c GEO profiles
PInt SPAC6B12.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC6B12.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC6B12.10c Fission yeast phenotypic data & analysis
SPD / RIKEN23/23C12Orfeome Localization Data
UniProtKB/SwissProtO14215DNA primase small subunit
ModBaseO14215Database of comparative protein structure models
STRINGO14215Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593765DNA primase catalytic subunit Spp1
RefSeq mRNANM_001019195972h- DNA primase catalytic subunit Spp1 (spp1), mRNA
European Nucleotide ArchiveCAB11078.1ENA Protein Mapping
UniParcUPI00001321B1UniProt Archive

Literature for spp1

Search: Europe PMC or PubMed

Release Version: PomBase:25_49 - 02 Feb 2015