spp1 (SPAC6B12.10c)


Gene Standard Namespp1 Characterisation Statuspublished
Systematic IDSPAC6B12.10c Feature Typeprotein coding
Synonymspri1 Name Description
ProductDNA primase catalytic subunit Spp1 Product Size454aa, 52.01 kDa
Genomic Location Chromosome I, 2430742-2428976 (1767nt); CDS:2430617-2429169 (1449nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
DNA primase activity4
Annotation ExtensionEvidenceWith/FromReference
metal ion binding750
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

DNA replication


GO Cellular Component
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
decreased vegetative cell population growthspp1-14652
spp1-4
inviable vegetative cell populationspp1-211453
spp1Δ
spp1-4
spp1-14
loss of viability at high temperaturespp1-2155
spp1-4
spp1-14
normal vegetative cell population growthspp1-21833

Cell Phenotype

Term NameGenotypesCount
abnormal cell cycle arrest in mitotic S phasespp1-1415
spp1-4
spp1-21
abnormal mitotic cell cyclespp1Δ858
abnormal mitotic cell cycle arrest with replicated DNAspp1Δ43
abnormal nuclear morphology during vegetative growthspp1-2175
spp1-14
spp1-4
abolished protein binding124
affecting pol1 and spp1spp1-21
affecting pol1 and spp1spp1-4
affecting pol1 and spp1spp1-14
cutspp1Δ125
decreased protein binding88
affecting pol1 and spp2spp1-14
affecting pol1 and spp2spp1-4
affecting pol1 and spp2spp1-21
decreased protein level during vegetative growth137
affecting spp1spp1-4
affecting spp1spp1-21
affecting spp1spp1-14
decreased response to mitotic G2 DNA damage checkpoint signalingspp1-42
spp1-21
spp1-14
elongated telomeresspp1-21173
spp1-9
spp1-4
spp1-14
increased protein kinase activity19
affecting cds1spp1-4
affecting cds1spp1-21
affecting cds1spp1-14
inviable after spore germination with elongated germ tubespp1Δ164
inviable after spore germination, multiple cell divisions, elongated cellspp1Δ120
inviable after spore germination, single or double cell divisionspp1Δ106
inviable elongated mononucleate vegetative cellspp1-2149
spp1-14
spp1-4
inviable elongated vegetative cellspp1Δ416
normal chromatin silencing at telomerespp1-912
septation following abnormal mitotic chromosome segregationspp1-21175
spp1-4
spp1-14
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in pol1 DNA polymerase alpha catalytic subunit
FYPO affected by mutation in spp1 DNA primase catalytic subunit Spp1
FYPO affected by mutation in spp2 DNA primase large subunit Spp2
FYPO localization affected by mutation in cdc23 MCM-associated protein Mcm10
FYPO localization affected by mutation in cdc45 DNA replication pre-initiation complex subunit Cdc45
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2430742..2430544, 2430459..2428976
Intron2430543..2430460
5' UTR2430742..2430618PMID:21511999
3' UTR2429168..2428976PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01896 Pfam IPR002755 DNA primase, small subunit 130 363 1
PTHR10536 HMMPANTHER IPR014052 DNA primase, small subunit, eukaryotic/archaeal 1 454 1
1v33A01 Gene3D 332 390 1
1v33A01 Gene3D 30 212 1
SSF56747 SuperFamily 33 388 1
TIGR00335 TIGRFAM IPR014052 DNA primase, small subunit, eukaryotic/archaeal 36 383 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.56 Da
Charge 3.50
Codon Adaptation Index 0.40
Isoelectric point 6.91
Molecular weight 52.01 kDa
Number of residues 454
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelconstant during GO:0000278Western blot evidencePMID:11160827

Quantitative Gene Expression

View graphical display of gene expression data for spp1 (SPAC6B12.10c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4129during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3734during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4074during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4042during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3820during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
701.69during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.2during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.63during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4605
conserved in eukaryotes4515
conserved in bacteria1001
conserved in metazoa3424
conserved in vertebrates3399
conserved in archaea238
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC6B12.10c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bypir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
affinity captured bypol1DNA polymerase alpha catalytic subunit Affinity Capture-WesternPMID:11027257
affinity captured byspb70DNA polymerase alpha B-subunit Affinity Capture-WesternPMID:15314153
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC6B12.10c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
rescued by overexpression ofalp14TOG/XMAP14 family protein Alp14 Dosage RescuePMID:15507118
synthetically rescuescrb2DNA repair protein Rad9 homolog Crb2 Synthetic RescuePMID:12589755
phenotype enhanced bychk1Chk1 protein kinase Phenotypic EnhancementPMID:11160827
synthetic lethal withtrt1telomerase reverse transcriptase 1 protein Trt1 Synthetic LethalityPMID:12697806
External References
Database Identifier Description
NBRP SPAC6B12.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC6B12.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC6B12.10c BioGRID Interaction Datasets
Expression Viewer SPAC6B12.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC6B12.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC6B12.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC6B12.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC6B12.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC6B12.10c Transcriptome Viewer (Bähler Lab)
GEO SPAC6B12.10c GEO profiles
PInt SPAC6B12.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC6B12.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC6B12.10c Fission yeast phenotypic data & analysis
Cyclebase SPAC6B12.10c.1 Cell Cycle Data
SPD / RIKEN23/23C12Orfeome Localization Data
UniProtKB/SwissProtO14215DNA primase small subunit
ModBaseO14215Database of comparative protein structure models
STRINGO14215Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593765DNA primase catalytic subunit Spp1
RefSeq mRNANM_001019195972h- DNA primase catalytic subunit Spp1 (spp1), mRNA

Literature for spp1

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016