spp1 (SPAC6B12.10c)

Gene Standard Namespp1 ChromosomeI
Systematic IDSPAC6B12.10c Gene Start2430742
Synonymspri1 Gene End2428976
ProductDNA primase catalytic subunit Spp1 Gene Length1767
Feature Typeprotein coding CDS Start2430617
Name Description CDS End2429169
Characterisation Statuspublished CDS Length1449

Ensembl Gene Location
  1. SPAC6B12.07c
  2. SPNCRNA.194
  3. SPNCRNA.825
  4. mug185
  5. rsa3
  6. trm10
  7. spp1
  8. drc1
  9. prl43
  10. SPATRNATHR.02
  11. tom70
  12. SPAC6B12.14c
  13. SPAC6B12.13
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000229cutno_name (unrecorded)unrecordedPECO:0000004PMID:11160827
FYPO:0002061inviable vegetative cell populationMicroscopyspp1delta (deletion)deletionPMID:20473289
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0003896DNA primase activityIEAIPR002755, IPR014052GO_REF:0000002
GO:0046872metal ion bindingIEAUniProtKB:KW-0479GO_REF:0000037
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0000723telomere maintenanceIMPPMID:12697806
GO:0006269DNA replication, synthesis of RNA primerIEAUniProtKB:KW-0639GO_REF:0000037
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0000790nuclear chromatinICGO:0005658GO_REF:0000001
GO:0005634nucleusIDAPMID:16823372
GO:0005658alpha DNA polymerase:primase complexTASPMID:11027257
GO:0043596nuclear replication forkICGO:0005658GO_REF:0000001
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
124307422430544
224304592428976

References

Region Start End Reference
five_prime_UTR24307422430618PMID:21511999
three_prime_UTR24291682428976PMID:21511999
three_prime_UTR24291682428976PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF01896 IPR002755 Pfam DNA primase, small subunit 130 363 1
G3DSA:3.90.920.10 Gene3D 30 212 1
G3DSA:3.90.920.10 Gene3D 332 390 1
SSF56747 SuperFamily 33 388 1
PTHR10536 IPR014052 hmmpanther DNA primase, small subunit, eukaryotic/archaeal 14 454 1
TIGR00335 IPR014052 tigrfam DNA primase, small subunit, eukaryotic/archaeal 36 383 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.56 Da
Charge 3.50
Isoelectric point 6.91
Molecular weight 52.01 kDa
Number of residues 454
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127		population_widePMID:23101633
experimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide701.69PMID:23101633
RNA levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide0.63PMID:23101633
experimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide2.2PMID:23101633
Species Distribution
DescriptionQualifierReference
predominantly single copy (one to one)
conserved in fungi
conserved in eukaryotes
conserved in bacteria
conserved in metazoa
conserved in vertebrates
conserved in archaea
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
crb2DNA repair protein Rad9 homolog, Rhp9 Synthetic RescuePMID:12589755
alp14TOG ortholog Alp14 Dosage RescuePMID:15507118
chk1Chk1 protein kinase Phenotypic EnhancementPMID:11160827
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
spb70DNA polymerase alpha B-subunit Affinity Capture-WesternPMID:15314153
pol1DNA polymerase alpha catalytic subunit Affinity Capture-WesternPMID:11027257
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External References
Database Identifier Description
NBRP SPAC6B12.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC6B12.10c Retrieval of eukaryotic orthologs
BioGrid SPAC6B12.10c BioGRID Interaction Datasets
Bähler Lab SPAC6B12.10c Cell Cycle Expression Profile
Bähler Lab SPAC6B12.10c Meiosis/Sporulation Expression Profies
Bähler Lab SPAC6B12.10c Pheromone response/mating expression profiles
Bähler Lab SPAC6B12.10c Environmental stress expression profiles
Bähler Lab SPAC6B12.10c Bähler Lab Transcriptome Viewer
Cyclebase SPAC6B12.10c Cell Cycle Data
PInt SPAC6B12.10c Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPAC6B12.10c Entrez Gene
WikiGene2543323DNA primase catalytic subunit Spp1
Uniprot_genenamePRI1
EntrezGene2543323DNA primase catalytic subunit Spp1
SPD / RIKEN23/23C12Orfeome Localization Data
UniProtKB/SwissProtO14215DNA primase small subunit
ModBaseO14215Database of comparative protein structure models
Pfam Protein DomainsO14215Pfam Domain Arrangement
RefSeq PeptideNP_593765DNA primase catalytic subunit Spp1
RefSeq mRNANM_001019195972h- DNA primase catalytic subunit Spp1 (spp1), mRNA
European Nucleotide ArchiveCAB11078ENA Protein Mapping
UniParcUPI00001321B1UniProt Archive

Literature for spp1
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013