spp1 (SPAC6B12.10c)


Gene Standard Namespp1 Characterisation Statuspublished
Systematic IDSPAC6B12.10c Feature Typeprotein coding
Synonymspri1 Name Description
ProductDNA primase catalytic subunit Spp1 Product Size454aa, 52.01 kDa
Genomic Location Chromosome I, 2430742-2428976 (1767nt); CDS:2430617-2429169 (1449nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0003896DNA primase activityIEAIPR002755GO_REF:00000024
GO:0046872metal ion bindingIEAUniProtKB-KW:KW-0479GO_REF:0000037748
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:1902981synthesis of RNA primer involved in mitotic DNA replicationIEAUniProtKB-KW:KW-0639GO_REF:00000378
GO:0000723telomere maintenanceIMPPMID:1269780644
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005658alpha DNA polymerase:primase complexTASPMID:110272576
GO:0000790nuclear chromatinICGO:0005658GO_REF:0000001279
GO:0005634nucleusIDAPMID:168233722740
GO:1990077primosome complexIEAUniProtKB-KW:KW-0639GO_REF:00000372
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopyspp1ΔNullPECO:0000005, PECO:0000137PMID:236978061331
Microscopyspp1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopyspp1ΔNullPECO:0000005, PECO:0000137PMID:23697806638
FYPO:0003165cutMicroscopynot recorded (unrecorded)PECO:0000004PMID:1116082780
FYPO:0001122elongated vegetative cellMicroscopynot recorded (unrecorded)PECO:0000004PMID:11160827657
FYPO:0000314inviable after spore germination with elongated germ tubeMicroscopyspp1ΔNullPECO:0000005, PECO:0000137PMID:23697806163
FYPO:0001042inviable after spore germination, single or double cell divisionMicroscopyspp1ΔNullPECO:0000005, PECO:0000137PMID:2369780695
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
124307422430544
224304592428976

UTRs

Region Coordinates Reference
five_prime_UTR2430742..2430618PMID:21511999
three_prime_UTR2429168..2428976PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01896 Pfam IPR002755 DNA primase, small subunit 130 363 1
PTHR10536 HMMPANTHER IPR014052 DNA primase, small subunit, eukaryotic/archaeal 5 454 1
1v33A01 Gene3D 30 212 1
1v33A01 Gene3D 332 390 1
SSF56747 SuperFamily 33 388 1
TIGR00335 tigrfam IPR014052 DNA primase, small subunit, eukaryotic/archaeal 36 383 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.56 Da
Charge 3.50
Isoelectric point 6.91
Molecular weight 52.01 kDa
Number of residues 454
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
4129during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
3734during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
4074during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
4042during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
3820during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
701.69during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2.2during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
0.63during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3393
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
chk1Chk1 protein kinase Phenotypic EnhancementPMID:11160827
alp14TOG ortholog Alp14 Dosage RescuePMID:15507118
crb2DNA repair protein Rad9 homolog, Rhp9 Synthetic RescuePMID:12589755
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
pol1DNA polymerase alpha catalytic subunit Affinity Capture-WesternPMID:11027257
spb70DNA polymerase alpha B-subunit Affinity Capture-WesternPMID:15314153
External References
Database Identifier Description
NBRP SPAC6B12.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC6B12.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC6B12.10c BioGRID Interaction Datasets
Expression Viewer SPAC6B12.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC6B12.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC6B12.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC6B12.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC6B12.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC6B12.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC6B12.10c Cell Cycle Data
GEO SPAC6B12.10c GEO profiles
PInt SPAC6B12.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC6B12.10c Peptides identified in tandem mass spectrometry proteomics experiments
WikiGene2543323DNA primase catalytic subunit Spp1
EntrezGene2543323DNA primase catalytic subunit Spp1
SPD / RIKEN23/23C12Orfeome Localization Data
UniProtKB/SwissProtO14215DNA primase small subunit
ModBaseO14215Database of comparative protein structure models
STRINGO14215Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593765DNA primase catalytic subunit Spp1
RefSeq mRNANM_001019195972h- DNA primase catalytic subunit Spp1 (spp1), mRNA
European Nucleotide ArchiveCAB11078ENA Protein Mapping
European Nucleotide ArchiveCAB11078.1ENA Protein Mapping
ReactomeREACT_202494
ReactomeREACT_214096
ReactomeREACT_219249
ReactomeREACT_222044
ReactomeREACT_225488
ReactomeREACT_225910
UniParcUPI00001321B1UniProt Archive

Literature for spp1

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014